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development of monoclonal-antibody-elisa, -dot-blot and -dip-stick immunoassays for humicola lanuginosa in rice.monoclonal antibodies (mabs) were raised against humicola lanuginosa, a thermophilic, saprophytic fungus that is commonly isolated from freshly harvested rice grains in indonesia. mice were immunized by direct injection into the peritoneum, without prior concentration, of fresh cell-free surface washings from a solid agar slant culture. hybridoma supernatants were screened by elisa using wells coated with a dilution of the immunogen. from one fusion 403 hybridoma clones were obtained yielding 52 ...19892614384
[isolation of imazalil and prochloraz resistant penicillium variable strains from a commercial citrus warehouse]."in vitro" sensitivity of two strains of penicillium variabile to different concentrations of imazalil and prochloraz has been studied. both strains were isolated from a citrus packing-house in which imazalil was used. these strains were resistant to both compounds showing a ed50 higher than 100 ppm.19873268294
pyrene degradation by yeasts and filamentous fungi.the saprotrophic soil fungi fusarium solani (mart.) sacc., cylindrocarpon didymum (hartig) wollenw, penicillium variabile sopp. and the yeasts rhodotorula glutinis (fresenius) harrison and rhodotorula minuta (saito) harrison were cultured in mineral medium with pyrene. the remaining pyrene concentrations were periodically determined during 20 incubation days, using hplc. to assess the metabolism of pyrene degradation we added 0.1 microci of [4,5,9,10] 14c-pyrene to each fungi culture and measure ...200211843531
isolation, purification, and properties of cellulases from aspergillus terreus and penicillium variabile. 196514282936
a note on the antifungal substance produced by penicillium variabile sopp. 196514294516
the glucose oxidase of penicillium variabile p16: gene cloning, sequencing and expression.isolation and characterization of the glucose oxidase (gox)-encoding gene from a penicillium variabile strain (p16) having a high level of gox activity and comparison of its expression with that of another strain of p. variabile (nrrl 1048) characterized by low gox activity.200414962046
pcr-rflp of its rdna for the rapid identification of penicillium subgenus biverticillium species.rflp of its rdna is proposed as a useful tool for molecular identification of the most common species of biverticillate penicillia. 60 isolates were analysed representing 13 species and 21 unique sequences were produced. the combination of five restriction enzymes was successful in separating 12 species. however, the variety penicillium purpurogenum var. rubrisclerotium remained indistinguishable from penicillium funiculosum. p. funiculosum appeared as the most confused species, being mis-identi ...200617196020
[the fungus penicillium variabile sopp 1912 isolated from permafrost deposits as a producer of rugulovasines].it has been established that relict fungi penicillium variabile sopp can synthesize clavine alkaloids, rugulovasines a and b, which are revealed in this species for the first time. submerged cultivation of the strain-producer revealed several microcycles of conidia formation. the synthesis of alkaloids was also of a cyclic character. the synchronism beween the cycles of rugulovasine biosynthesis and conidia formation was revealed. zinc ions stimulated fungal growth but had a negative effect on t ...200617205797
[growth and biosynthesis of rugulovasines in penicillium variabile sopp 1912].production of clavine alkaloids rugulovasines by p. variabile did not depend on the habitat of the producers. during submerged cultivation on a simple synthetic medium in early growth stages, microcyclic conidiation was observed in the tested fungi; its presence or absence, as well as the activity of the cultures as to biosynthesis of rugulovasines, depended on the composition of the culture medium. on a complex medium supplemented with peptone, conidiation occurred but was considerably suppress ...200818825977
molecular fingerprinting by pcr-denaturing gradient gel electrophoresis reveals differences in the levels of microbial diversity for musty-earthy tainted corks.the microbial community structure of cork with marked musty-earthy aromas was analyzed using denaturing gradient gel electrophoresis of amplified ribosomal dna. cork stoppers and discs were used for dna extraction and were analyzed by using selective primers for bacteria and fungi. stoppers clearly differed from discs harboring a different fungal community. moreover, musty-earthy samples of both types were shown to have a specific microbiota. the fungi penicillium glabrum and neurospora spp. wer ...200919201983
[screening and application of phosphate-dissolving microorganism suitable for corn production].to obtain phosphate-dissolving strains which can be suitable for corn production.201021365915
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