| [conditions of dextranase formation by penicillium funiculosum 15]. | the influence of the following factors on the synthesis of extracellular dextranase by pen. funiculosum 15 has been studied: the quantity and age of the inoculum, ph of the cultivation medium, stimulants of the microbial growth, cultivation temperature and time. the optimal amount of dextranase has been found to form under the following conditions: inoculum--3 day mycelium constituting 4%, cultivation time--4 to 7 days, temperature--28 to 29 degrees c, initial ph of the medium--6.0. | 1975 | 236553 |
| [stabilization of dextranase from penicillium funiculosum and fusarium solani during heating and freeze-drying]. | freeze-drying of highly purified dextranse from penicillium funiculosum and fusarium solani was accompanied by 90% losses of enzyme activity and solubility. many carbohydrates were tested as stabilizers, e.g. glucose, maltose, lactose, polyglucine, dextranase hydrolyzate of polyglucine as well as mannitol and ammonium sulfate. polyglucine, its hydrolyzate, and glucose proved most effective stabilizers. the stabilizing effect of polyglucine hydrolyzate of dextranase during its heating and freeze- ... | 1979 | 538015 |
| the influence of water-logging stage upon the paddy soil mycoflora in the ukraine. | the soil fungal flora of paddy soils from the southern districts of the ukraine was investigated. from 287 soil samples 170 species were isolated. they belonged to the following genera: oomycetes 1.2%, zygomycetes 8.8%, ascomycetes 5.8%, deuteromycetes 84.2%. certain fungi were found to be dominant forms, among them mortierella alpina peyron., penicillium funiculosum thom, p. rugulosum thom, p. chrysogenum thom, acremonium strictum w. gams, acr. breve w. gams, alternaria alternata (fr.) riessler ... | 1978 | 726714 |
| distributional patterns of mesophilous and thermophilous microfungi in two bahamian soils. | this study focuses on the characteristics displayed by mesophilous and thermophilous microfungal populations occurring in two tropical monodominant plant communities, a cocos nucifera grove and a casuarina equisetifolia forest, that provide distinctly different edaphic conditions. the mesophilous population sampled at 25 degrees c by the dilution plate method and the thermophilous population that developed on soil plates incubated at 45 degrees c consisted of 1693 isolates representing 60 specie ... | 1975 | 1214847 |
| activity staining of endoglucanases in polyacrylamide gels. | the endoglucanases of penicillium funiculosum were analyzed for the presence of multiple forms using a modified version of the congo red method. postelectrophoretic slab gels were directly incubated in a solution of carboxymethylcellulose for a period as short as 15 min and then the activities were visualized by staining with congo red. ten distinct bands of clearances were obtained indicating the presence of at least as many multiple forms. | 1992 | 1280921 |
| saprophytic and keratinophilic fungi isolated from desert and cultivated soils continuously exposed to cement dust particles in egypt. | forty soil samples collected from desert (uncultivated) and cultivated soils, exposed continuously to cement dust were screened for their content of saprophytic and keratinophilic fungi using the hair baiting technique and the dilution plate method. using the hair baiting technique, 21 genera and 31 species were collected from 20 samples of each of desert (15 genera and 21 species) and cultivated (17 genera and 24 species) soils, on sabouraud's dextrose agar. these included chrysosporium keratin ... | 1992 | 1325092 |
| immunological and molecular genetic analysis of the cellulase component from penicillium funiculosum. | following immunization of rabbits, the antiserum was initially analyzed for antiendoglucanase activity using dot-blot elisa methods. when compared with the preimmune serum, the antiserum showed strong response even at 25 ng concentration. the specificity of the polyclonal antibodies, raised against the partially purified endoglucanase component of penicillium funiculosum, was determined by oüchterlony double diffusion. rocket electrophoresis confirmed the presence of only two types of antigens i ... | 1991 | 1796811 |
| laminarinase from penicillium funiculosum and its role in release of beta-glucosidase. | an extracellular laminarinase (1----3)-beta-glucan glucohydrolase (ec 3.2.1.6) was purified from culture filtrates of penicillium funiculosum. it was homogeneous on polyacrylamide gel electrophoresis in the presence and absence of sodium dodecyl sulfate. it had a mr of 14,000 and isoelectric point of ph 4.2. the apparent km value for lamimarinase was 8.3 mg/ml and vmax was 8 mumol/min/mg. the distribution of beta-glucosidase activity in two different species of penicillium showed that p. funicul ... | 1991 | 1904246 |
| extracellular poly(3-hydroxybutyrate) depolymerase from penicillium funiculosum: general characteristics and active site studies. | an extracellular poly(3-hydroxybutyrate) (phb) depolymerase has been isolated from penicillium funiculosum cultural medium by a single hydrophobic column chromatography. the enzyme is a glycoprotein composed of a single polypeptide chain with a molecular mass of about 37,000 da as analyzed by denatured sodium dodecyl sulfate-polyacrylamide gel electrophoresis and by native gel filtration on sephadex g-100. its optimum activity occurs at ph 6.0. it has an isoelectric point of 5.8 and has a km for ... | 1991 | 1929416 |
| tan-931, a novel nonsteroidal aromatase inhibitor produced by penicillium funiculosum no. 8974. i. taxonomy, fermentation, isolation,characterization and biological activities. | a novel nonsteroidal aromatase inhibitor, tan-931, was isolated from the culture filtrate of a soil isolate fungus, no. 8974. the strain was identified as penicillium funiculosum no. 8974. tan-931 inhibited human placental and rat ovarian aromatase activity, and the ic50 value was 17.2 and 162 microm, respectively. the inhibition of human placental aromatase was uncompetitive with respect to androstenedione conversion with a ki value of 40 microm. when tan-931 was subcutaneously administered at ... | 1991 | 2071487 |
| tan-931, a novel nonsteroidal aromatase inhibitor produced by penicillium funiculosum no. 8974. ii. structure elucidation, chemical modification and biological activity. | the structure of tan-931, a novel nonsteroidal aromatase inhibitor, was determined by chemical reactions and spectral analyses including 2d nmr experiments to be 4-(2,6-dihydroxybenzoyl)-3-formyl-5-hydroxybenzoic acid. several derivatives of tan-931 were prepared, and it was found that the 3-formyl and 2'- and/or 6'-hydroxyl groups play an important role in its inhibitory activity. among the compounds synthesized, 4-(2,6-dihydroxybenzoyl)-3-formyl-5-methoxy-n,n-dimethyl-benzamide was found to be ... | 1991 | 2071488 |
| cloning of the cellulase gene from penicillium funiculosum and its expression in escherichia coli. | a gene of penicillium funiculosum encoding an endoglucanase was cloned and expressed in escherichia coli using the lacz promoter of vector puc 18. the gene product hydrolyzed carboxymethyl cellulose and showed strong cross reactivity with p. funiculosum anticellulases. | 1990 | 2182377 |
| studies on the mycoflora of aswan high dam lake, egypt: monthly variations. | fifty-one species and one variety appertaining to twenty one genera of mesophilic fungi were recovered from the monthly samples of marginal water (44 species, 1 variety and 18 genera) and submerged mud (78 species, 1 variety and 30 genera) of aswan high dam lake during the period from july 1985 to december 1986. the most common species were aspergillus fumigatus, a. flavus, a. terreus, a. niger and penicillium funiculosum. the highest fungal populations were almost detected either in october, in ... | 1990 | 2352135 |
| studies on the mycoflora of aswan high dam lake, egypt: vertical fluctuations. | the fungal population of aswan high dam lake showed marked vertical variations during the period of study which extended from july 1985 to december 1986. high fungal counts were observed at the surface water which were mainly due to the high counts of aspergillus fumigatus and a. terreus. going deeper the fungal population decreased till 30 meters, then gradually increased to reach its maximum at the 70 meter depth (near the bottom of the lake). such increase was basically due to the high popula ... | 1990 | 2370640 |
| keratinophilic fungi associated with rabbit claws in egypt. | using the soil plate technique, thirty one species appertaining to twenty three genera were recovered from 160 samples of rabbit claws which were collected from a rabbit farm at assiut. of the true keratinophilic fungi chrysosporium tropicum was the most prevalent where it colonized 56.25% of the samples. microsporum gypseum was rarely isolated in this study. among the non-keratinophilic fungi penicillium funiculosum. p. jenseni and paccilomyces lilacinus were of low incidence (20%, 19.37% and 1 ... | 1989 | 2600778 |
| a novel acid phosphatase excreted by penicillium funiculosum that hydrolyzes both phosphodiesters and phosphomonoesters with aryl leaving groups. | an acid phosphatase has been purified from the culture broth of penicillium funiculosum by procedures including sp-sephadex column chromatography and sephacryl s-200 gel filtration. the phosphatase appears to be a 76 kda heterodimer composed of 51 and 26 kda subunits on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the enzyme hydrolyzes both phosphodiesters and phosphomonoesters, but only those with aryl leaving groups. at the early phase of degradation of bis-p-nitrophenyl phosphat ... | 1989 | 2753875 |
| [effect of the entomopathogenic fungus penicillium funiculosum on the reproduction of the causative agent of plague in the body of fleas]. | | 1988 | 3173243 |
| induction and catabolite repression of cellulase in penicillium funiculosum. | the regulation of endoglucanase synthesis in penicillium funiculosum is investigated using a method based on the viscosity lowering effect on carboxy methyl cellulose (cmc) by endoglucanase. cellobiose (1 mg/l) causes induction, whereas glucose (5 g/l) does not repress the enzyme formation. lactose (5 g/l) has no effect on the synthesis of cellulase. avicel and cellulose powder (cp) are the best inducers of cellulase and xylanase activity. both endoglucanase and xylanase activity were induced by ... | 1988 | 3228292 |
| mode of action and synergism of cellulases from penicillium funiculosum. | a 1,4-beta-d-glucan cellobiohydrolase (ec 3.2.1.91) and 1,4-beta-d-glucan glucanohydrolase (ec 3.2.1.4) were purified from the culture filtrates of penicillium funiculosum by using preparative isoelectric focusing. both the enzymes were homogeneous on polyacrylamide gel with and without sodium dodecyl sulphate. the mol wt of the cellobiohydrolase and endoglucanase were 14,400 and 25,000 respectively. the purified enzymes were free of beta-glucosidase activity. acting in isolation, the cellobiohy ... | 1988 | 3228293 |
| sq 30,957, a new antibiotic produced by penicillium funiculosum. taxonomy, fermentation, isolation, structure determination, synthesis and antibacterial activity. | a new antibiotic, sq 30,957, 4-diazo-3-methoxy-2,5-cyclohexadien-1-one, has been isolated from fermentation broths of penicillium funiculosum. the structure (1) was deduced from its spectroscopic properties and its degradation reaction. sq 30,957 has excellent activity against anaerobic bacteria such as clostridium and bacteroides and has moderate activity against aerobic bacteria. the compound has an ld50 of less than 17 mg/kg in mice by intraperitoneal administration. | 1986 | 3759656 |
| studies on dextranase. v. activation of dextranase from penicillium funiculosum iam 7013 by co2+. | | 1974 | 4219754 |
| studies on dextranase. iii. action patterns of dextranase from penicillium funiculosum on substrate and inhibition on hydrolysis reaction by substrate analogues. | | 1974 | 4434582 |
| [synthetic medium for the biosynthesis of dextranase by penicillium funiculosum 15]. | | 1974 | 4453549 |
| [comparative antiviral activity of the penicillium funiculosum virus and its rna]. | | 1974 | 4465667 |
| studies on dextranase. iv. immunobilization of dextranase from penicillium funiculosum iam 7013. | | 1974 | 4468115 |
| [experimental studies on a dextranase from penicillium funiculosum]. | | 1973 | 4512708 |
| [viruslike particles in the mycelium of penicillium funiculosum m 2319]. | | 1972 | 4670689 |
| dextranase production from penicillium funiculosum. | | 1973 | 4730510 |
| studies on dextranase. purification of dextranase from penicillium funiculosum and its enzymatic properties. | | 1973 | 4731965 |
| [some physicochemical properties of the rna of the virus of penicillium funiculosum and its effect on the tobacco mosaic virus]. | | 1973 | 4791362 |
| [effect of preparations of the virus of penicillium funiculosum on the tobacco mosaic virus]. | | 1973 | 4791363 |
| [morphology of penicillium funiculosum thom. affected by virus]. | | 1971 | 5152476 |
| inducers of interferon and host resistance. i. double-stranded rna from extracts of penicillium funiculosum. | | 1967 | 5233474 |
| purification and some properties of dextranase from penicillium funiculosum. | | 1970 | 5275858 |
| the structure of funicone. a new metabolite from penicillium funiculosum thom. | | 1970 | 5448440 |
| isolation of a pure dextranase from penicillium funiculosum. | a dextranase, produced by penicillium funiculosum, was purified 1,000-fold to yield the enzyme which was demonstrated by gel electrophoresis and electrofocusing to be a homogeneous protein. the purification method included acetone partition, ammonium sulfate fractionation, gel filtration, iron defecation and precipitation, and diethylaminoethyl-cellulose chromatography. the pure enzyme was also obtained by preparative gel electrophoresis. gel-permeation chromatography indicates a molecular weigh ... | 1970 | 5485722 |
| toxicity to chicks of aspergillus and penicillium species isolated from moldy pecans. | isolates of aspergillus chevalieri, a. flavus, a. ochraceus, a. repens, and penicillium funiculosum and complexes of p. citrinum-p. implicatum isolated from moldy pecan meats were toxic to chicks. | 1971 | 5564681 |
| an antiviral substance from penicillium funiculosum. iv. inquiry into the mechanism by which helenine exerts its antiviral effect. | 1. helenine injected intraperitoneally 24 hr prior to a regularly fatal dose of semliki forest virus saves most of the mice to which it is administered. 2. mice saved by helenine develop no viral immunity and regularly succumb when rechallenged 2 wk later with the same dose of virus from which they were originally saved. 3. the time during which helenine is optimally effective in protecting mice from death by semliki forest virus covers a period of approximately 36 hr beginning after about 12 hr ... | 1966 | 5905239 |
| an antiviral substance from penicillium funiculosum. vii. an attempt to determine whether the material responsible for the antipassive immunity effect exhibited by mice injected with helenine is an interferon. | helenine, ndv, and statolon, all known inducers of interferon in mice, all exerted a marked antiviral effect against semliki forest virus. this av effect was, so far as can be demonstrated, mediated through, the induced interferon. the same three materials also exerted a marked antipassive immunity effect. all the evidence that can be brought to bear indicates that this api effect like the av effect is mediated through interferon known to be induced by the three materials. if the api effect does ... | 1966 | 5926302 |
| an antiviral substance from penicillium funiculosum. v. induction of interferon by helenine. | helenine, a substance obtained from broth cultures of penicillium funiculosum and known to exert a protective effect in vivo in experimental animals against several unrelated viruses, has been shown to elicit the formation in cell cultures and in intact mice of an inhibitor of viral plaque formation. because the biological and chemical characteristics of the viral inhibitor induced by helenine are similar to those of interferon, it is suggested that the antiviral effect of helenine may be mediat ... | 1966 | 5933468 |
| the presence in penicillium funiculosum of an inhibitor to the antiviral agent helenine. | the present findings indicate that the wide fluctuation observed in the antiviral activity of various crude helenine preparations may be attributable to the presence of varying amounts of inhibitor. antiviral activity could be enhanced by removal of the inhibitor. a meaningful value for helenine titer in a preparation clearly should be determined in the inhibitor-free zone. | 1966 | 5937058 |
| an antiviral substance from penicillium funiculosum. vi. prevention of the establishment of passive immunity to semliki forest virus infection in mice by helenine. | 1. helenine prevents the establishment in mice of passive viral immunity by anti-semliki serum of swine, rabbit, or guinea pig origin. 2. a period of 12 days must elapse, between the antiviral serum administration and challenge with virus, for prevention of the establishment of passive immunity to become apparent. this period is believed to correspond to that in which injected antibody persists in circulation in the injected host. 3. helenine is effective in preventing the establishment of passi ... | 1966 | 5944346 |
| utilization of cheap carbohydrate sources for production of calcium gluconate by penicillium funiculosum mutant mn238. | | 1984 | 6480059 |
| [hydrolysis of streptococcal polysaccharides by micromycete dextranases]. | the effect of dextranase enzyme preparations obtained from penicillium piscarium bim g-102, penicillium funiculosum, aspergillus insuetus g-116 and aspergillus ustus on polysaccharides synthesized by cariesogenic streptococcus sanguis and streptococcus mitis was being studied. according to the data obtained dextranases from p. piscarium, p. funiculosum and asp. ustus can be considered as a promising anticarious agent. | 1984 | 6540443 |
| [effect of the mineral components of czapek's medium on the growth and cellulosolytic activity of penicillium funiculosum thom 28]. | | 1980 | 6776389 |
| [carbohydrate component of penicillium funiculosum dextranase]. | the carbohydrate composition of dextranase from penicillium funiculosum 15, as well as the composition of products of dextran deep hydrolysis by the enzyme were studied. the products are normally used to stabilize the enzyme during its purification. using the methods available, it was possible to identify only part of strongly bound (adsorbed) carbohydrates. it was found that dextranase from pen. funiculosum 15 contained two types of carbohydrates strongly bound with protein: adsorbed and covale ... | 1983 | 6835949 |
| rhizosphere fungi of five plants in egypt. | 75 species which belong to 25 genera were collected during this investigation. all of these fungi were previously isolated from egyptian soils and other sources. the total count and the number of genera and species in the rhizosphere soil of the 5 plants were regularly higher than in non-rhizosphere soil. the composition of the rhizosphere fungus flora was considerably influenced by the type and the age of the plant. the most prevalent fungi in the rhizosphere of the test plants after 45, 90, an ... | 1980 | 7424047 |
| the isolation, purification and properties of the cellobiohydrolase component of penicillium funiculosum cellulase. | 1. a cellobiohydrolase component was isolated from a penicillium funiculosum cellulase preparation by chromatography on deae-sephadex, and purified by isoelectric focusing. 2. purified in this way, the enzyme was homogeneous as judged by electrophoresis on sodium dodecyl sulphate/polyacrylamide gels and isoelectric focusing in polyacrylamide gels. 3. acting in isolation, the enzyme had little hydrolytic activity to highly ordered celluloses such as cotton fibre, but, when recombined in the origi ... | 1980 | 7458906 |
| effect of n-nitrosoamines and nitrosationable pesticide tetramethylthiuram disulfide on soil microbiocenosis and potato yield: a model system. | the effect of carcinogenic n-nitrosoamines (na), na-producing tetramethylthiuram disulfide (tmtd) pesticide, and of various doses of nitrogen fertilizers (90, 180, and 270 kg/ha) on the forming and vital functions of microbiocenosis of light gray forest soil was studied. the quantity, nitrifying activity, and gas production of nitrifying bacteria increased under the influence of na. the nitrosodimethylamine (ndma) and the nitrosodibutilamine (ndba) changed the correlation between the number of s ... | 1994 | 7658332 |
| production of hormones by fungi. | twenty species of filamentous fungi were tested for their gibberellin and sterol production, with emphasis on the action of some factors on these products. while all cultures produced both gibberellin and sterol in variable amounts, aspergillus flavus, a. niger, a. ochraceus, cladosporium cladosporioides, gibberella zeae, penicillium funiculosum, p. italicum and p. rubrum were the best producers. the isolates of a. niger isolated from soil and p. italicum isolated from citrus fruit were selected ... | 1994 | 7740983 |
| bioproduction of an extract from penicillium funiculosum thom with activity against ceratitis capitata and tetranychus urticae. | the bioproduction conditions of a crude extract from a strain of penicillium funiculosum thom, which shows activity against ceratitis capitata and tetranychus urticae, have been optimized. the efficacy degree was 95% (c. capitata) and 100% (t. urticae), according to abbot. this is the first report on the pesticide activity of an extract from p. funiculosum against these species. the best yields were obtained when p. funiculosum was grown in raulin-thom medium at ph 6.5 with sucrose candy as the ... | 1993 | 7763929 |
| physiological studies on xylanase production by penicillium funiculosum on some agricultural wastes. | studies were carried out on production of xylanase enzyme from p. funiculosum nrce-629 using a number of alkali-treated lignocellulosic wastes as carbon and energy sources as compared to pure xylan polymer. sugar cane bagasse, rice straw and wheat straw yielded enzyme levels higher than that obtained on pure xylan. physiological studies on enzyme formation using those three agricultural wastes revealed that optimum enzyme yields are obtained in media containing 2% substrate concentration. the en ... | 1993 | 8368028 |
| immobilization of beta-glucosidase from penicillium funiculosum on nylon powder. | beta-glucosidase from penicillium funiculosum was immobilized on nylon powder previously activated with triethyloxonium tetrafluoroborate, 1,2-diaminoethane and glutaraldehyde. the activation of the nylon powder and the immobilization processes were studied and optimized for the enzyme and the matrix. a high activity retention (67%) was obtained using the activation and immobilization conditions finally selected. | 1993 | 8439404 |
| in vitro determination of ruminal protein degradability of alfalfa and prairie hay via a commercial protease in the presence or absence of cellulase or driselase. | ruminal protein degradation of alfalfa (2.62% n, 49.6% ndf, and in vivo undegradable intake protein [uip] = 16.4% of cp) and prairie hay (.88% n, 69.4% ndf, and in vivo uip = 44.5% of cp) was estimated using the streptomyces griseus protease (sgp) in vitro method with or without pretreatment with two carbohydrases: cellulase from penicillium funiculosum or driselase from basidiomycetes. driselase is a broad-spectrum carbohydrase. incubating forage samples for 48 h with cellulase or driselase at ... | 1997 | 9263071 |
| mycological and some physiological studies of keratinophilic and other moulds associated with sheep wool. | wool samples of 50 healthy sheep collected from different localities at ar' ar in saudi arabia were examined for the presence of keratinophilic and other fungi using the hair plate technique. forty-eight species and 2 varieties belonging to 20 genera were isolated on sabouraud's dextrose agar at 25 degrees c. two species of true dermatophytes were isolated: trichophyton terrestre and t. rubrum in low frequency of occurrence. chrysosporium, a well-know keratinophilic genus, was the predominant ge ... | 1997 | 9265769 |
| effect of vapor phase corrosion inhibitor on microbial corrosion of aluminum alloys. | vapor phase corrosion inhibitors were used to investigate the antimicrobial activities and anticorrosion of aluminum alloy. aspergillus flavus, a. niger, a. versicolor, chaetomium globosum and penicillium funiculosum had moderate to abundant growth on the aluminum alloy aa 1100 at aw 0.901, while there was less growth at aw 0.842. high humidity stimulated microbial growth and induced microbial corrosion. dicyclohexylammonium carbonate had a high inhibitory effect on the growth of test fungi and ... | 1996 | 10592784 |
| purification and partial characterization of a novel glucanhydrolase from lipomyces starkeyi ksm 22 and its use for inhibition of insoluble glucan formation. | a novel glucanhydrolase from a mutant of lipomyces starkeyi atcc 74054 was purified. the single protein (100 kda) showed either dextranolytic or amylolytic activity. we referred to the glucanhydrolase as a dxamase. the dxamase was produced in a starch medium and it was 3.75-fold more active for hydrolysis of the purified insoluble-glucan of streptococcus mutans than penicillium funiculosum dextranase. aggregation of s. mutans cells with dextran and adherence to glass were eliminated by incubatin ... | 2000 | 10737173 |
| [effect of conditions of culturing penicillium funiculosum g-15 on production of extracellular glucose oxidase]. | effects of conditions of penicillium funiculosum g-15 cultivation on the production of extracellular glucose oxidase were studied. the data showed that surface and submerged methods of cultivation can be used for obtaining a glucose oxidizing enzyme. the optimum conditions for submerged cultivation (25 degrees c, initial ph 5.0, and aeration of 3 l/l per min) and surface cultivation (temperature 25 degrees c and initial ph 4.0) providing the maximum levels of glucose oxidase synthesis were deter ... | 2000 | 10780009 |
| [preparation and characteristics of mutant strains of penicillium funiculosum--producers of glucose oxidase]. | after the mutagenesis of penicillium funiculosum with uv light and n-nitroso-n-methylurea, 83 of 2237 grown colonies were surrounded with increased zones of glucose oxidase diffusion. analysis of the glucose oxidase activity of selected mutant strains grown in submerged cultures allowed 18 mutant strains to be obtained whose glucose oxidase activity was 5-153% higher (in a medium with glucose) and 4-83% higher (in a medium with sucrose) than that of the parent strain. two of these mutant strains ... | 2000 | 11008685 |
| a modular esterase from penicillium funiculosum which releases ferulic acid from plant cell walls and binds crystalline cellulose contains a carbohydrate binding module. | an esterase was isolated from cultures of the filamentous fungus penicillium funiculosum grown on sugar beet pulp as the sole carbon source. the enzyme (ferulic acid esterase b, faeb) was shown to be a cinnamoyl esterase (ce), efficiently releasing hydroxycinnamic acids from synthetic ester substrates and plant cell walls, and bound strongly to microcrystalline cellulose. a gene fragment was obtained by pcr using partial amino-acid sequences obtained from the pure enzyme and used to a probe a p. ... | 2000 | 11082184 |
| factors affecting the production of (-)-mitorubrinic acid by penicillium funiculosum. | the optimum conditions for the production of (-)-mitorubrinic acid by mutant strain of penicillium funiculosum ccm f-8080 were examined in shaken flask cultures. the highest production of this metabolite was achieved in a medium with glucose as the sole carbon source and (nh4)2hpo4 as the nitrogen source. the most suitable concentrations of the given sources for production of (-)-mitorubrinic acid are 80 g/l glucose and 2 g/l (nh4)2hpo4 (maximal production, 814.9 mg/l). we also observed the infl ... | 2000 | 11199497 |
| [the use of micromycetes for cleaning parts of aircraft]. | the mycelial fungi penicillium funiculosum, p. citrinum, p. expansum, p. chrysogenum, aspergillus ochraceus, a. alliaceus, a. luchaensis, a. flavus, and a. niger were isolated from enrichment cultures. these fungi actively destruct carbon deposits formed during exploitation of aircraft. a biotechnological method for removing fouling from parts of aircraft engines (pae) was developed. this method is less laborious, more rapid and ecologically clean than contemporary chemical methods. scanning mic ... | 2001 | 11234409 |
| or-1--a mixture of esters of glyceric acid produced by penicillium funiculosum and its antitrypsin activity. | a mixture of related metabolites (denoted or-1) was isolated from the fermentation broth of penicillium funiculosum together with mitorubrinic acid. structurally or-1 is glyceric acid esterified with c14-c18 fatty acids. steady-state studies revealed that or-1 behaved like an uncompetitive trypsin inhibitor with ki 17.6 mumol/l. | 2001 | 11501469 |
| cleavage of the xylosyl serine linkage between a core peptide and a glycosaminoglycan chain by cellulases. | we previously found that endo-beta-xylosidase from patinopecten is an endo-type glycosidase that cleaves the xylosyl serine linkage between a glycosaminoglycan chain and its core protein (takagaki, k., kon, a., kawasaki, h., nakamura, t., tamura, s., and endo, m. (1990) j. biol. chem. 265, 854-860). screening for endo-beta-xylosidase activity in several cellulases detected this activity in the enzymes from aspergillus niger, penicillium funiculosum, trichoderma reesei, trichoderma viride, and ir ... | 2002 | 11877400 |
| degradation of pyrene by indigenous fungi from a former gasworks site. | indigenous fungi isolated from soil of a former gasworks site were investigated in submerged cultures with pyrene as the sole carbon source. five fungal strains capable of degrading pyrene included one strain of trichoderma harzianum and four strains with characteristics of the genus penicillium. these are identified as penicillium simplicissimum, penicillium janthinellum, penicillium funiculosum and penicillium terrestre. a maximum of 75% of 50 mg l(-1) and 67% of 100 mg l(-1) of pyrene was rem ... | 2002 | 12044679 |
| [selection of penicillium funiculosum strains with high glucose oxidase activity]. | metabolic inhibitors, riboflavin, and end products of glucose oxidation were shown to hold much promise for the selection of penicillium funiculosum mutant strains with a high glucose oxidase activity. the incidence of positive mutations was highest in clones resistant to sodium azide, riboflavin, and beta-d-glucono-delta-lactone. enzyme activity in penicillium funiculosum mutants was studied under conditions of submerged cultivation. the intensity of glucose oxidase synthesis in seven cultures ... | 2002 | 12068579 |
| a family 11 xylanase from penicillium funiculosum is strongly inhibited by three wheat xylanase inhibitors. | steady-state kinetic approaches were used to investigate the binding of a novel penicillium funiculosum xylanase, xync, with three known xylanase inhibitor proteins from wheat (triticum aestivum). the xylanase gene (xync) was cloned from a p. funiculosum genomic library and the deduced amino acid sequence of xync exhibited high sequence similarity with fungal family 11 xylanases. xync was overexpressed in p. funiculosum and the product (xync: m(r)=23.6 kda; pi=3.7) purified and shown to efficien ... | 2002 | 12147340 |
| use of a histone h4 promoter to drive the expression of homologous and heterologous proteins by penicillium funiculosum. | two genes encoding histone h4 (h4.1 and h4.2) from penicillium funiculosum have been cloned and characterised. structurally, the histone h4.1 gene is divergently linked to the histone h3 gene and the two genes are separated by approximately 800 bp. the transcription of the histone h4.1 and h4.2 genes in p. funiculosum appears to be distinctively regulated. histone h4.1 mrna showed a high steady-state level during the early stages of batch culture that decreased as growth reached the stationary p ... | 2002 | 12466887 |
| physicochemical characterization and antimicrobial properties of rhamnolipids produced by pseudomonas aeruginosa 47t2 ncbim 40044. | pseudomonas aeruginosa 47t2, grown in submerged culture with waste frying oil as a carbon source, produced a mixture of rhamnolipids with surface activity. up to 11 rhamnolipid homologs (rha-rha-c(8)-c(10); rha-c(10)-c(8)/rha-c(8)-c(10);rha-rha-c(8)-c(12:1); rha-rha-c(10)-c(10); rha-rha-c(10)-c(12:1); rha-c(10)-c(10); rha-rha-c(10)-c(12)/rha-rha-c(12)-c(10); rha-c(10)-c(12:1)/rha-c(12:1)-c(10); rha-rha-c(12:1)-c(12); rha-rha-c(10)-c(14:1); rha-c(10)-c(12)/rha-c(12)-c(10)) were isolated from cult ... | 2003 | 12474254 |
| comparison of modular and non-modular xylanases as carrier proteins for the efficient secretion of heterologous proteins from penicillium funiculosum. | genes encoding three enzymes with xylanase activity from the filamentous fungus penicillium funiculosum are described. two of the encoded xylanases are predicted to be modular in structure with catalytic and substrate-binding domains separated by a serine and threonine-rich linker region; the other had none of these properties and was non-modular. in order to develop p. funiculosum as a host for the secreted production of heterologous proteins, each of the xylanases was assessed for use as a car ... | 2003 | 12664153 |
| an antiviral substance from penicillium funiculosum. i. effect upon infection in mice with swine influenza virus and columbia sk encephalomyelitis virus. | a culture of p. funiculosum isolated on guam proved capable of elaborating a substance which exerted a favorable therapeutic effect against swine influenza virus infections in white mice. the culture was extremely variable and irregular in its production of the antiviral substance, and during maintenance in the laboratory for several years gradually lost this property. efforts to restore it were unsuccessful. subsequently it was found that the mold elaborated a substance, now designated helenine ... | 1953 | 13052822 |
| an antiviral substance from penicillium funiculosum. ii. effect of helenine upon infection in mice with semliki forest virus. | helenine exerts a therapeutic effect against semliki forest virus infections of mice. cures, that is to say the survival of treated animals, were more frequently observed in semliki forest virus infections than they were in sk virus infections. it is believed that this difference in end-result probably represented only a quantitative difference in the therapeutic effect of helenine against these two viruses and not a qualitative difference in its mechanism of therapeutic action. the findings rep ... | 1953 | 13052823 |
| an antiviral substance from penicillium funiculosum. iii. general properties and characteristics of helenine. | helenine is moderately stable in solution at refrigerator temperature and can be kept for long periods of time without evident loss of activity if stored frozen at the temperature of solid co(2). it is filterable through a seitz pad but not dialyzable. crude sps preparations of helenine do not lose activity when dried from the frozen state. some conditions are described, however, which influence the preservation or inactivation of acetone-precipitated helenine when freeze-dried. helenine is part ... | 1953 | 13052824 |
| studies on the effect of beers of penicillium funiculosum against columbia sk virus infections in mice. | | 1957 | 13521920 |
| enzymatic browning and biochemical alterations in black spots of pineapple [ananas comosus (l.) merr.]. | penicillium funiculosum thom. was consistently isolated from pineapple-infected fruitlet (black spots). polyphenol oxidase, peroxidase, and laccase activities were determined in extracts from contiguous and infected fruitlets. healthy fruitlets showed a rather high level of polyphenol oxidase (optimum ph 7.0), and this activity was tremendously increased (x 10) in contiguous infected fruitlets. furthermore, infected fruitlets also exhibited laccase activity (optimum ph 4.0), while peroxidase was ... | 2003 | 14506857 |
| [intracellular glucose oxidase from penicillium funiculosum 46.1]. | a method for isolating extracellular glucose oxidase from the fungus penicillium funiculosum 46.1, using ultrafiltration membranes, was developed. two samples of the enzyme with a specific activity of 914-956 iu were obtained. the enzyme exhibited a high catalytic activity at ph above 6.0. the effective rate constant of glucose oxidase inactivation at ph 2.6 and 16 degrees c was 2.74 x 10(-6) s-1. this constant decreased significantly as ph of the medium increased (4.0-10.0). the temperature opt ... | 2003 | 14520960 |
| [isolation and properties of glucose oxidase from penicillum funiculosum 433]. | a method for isolation of extracellular glucose oxidase from penicillium funiculosum 433 and its purification is proposed. the enzymatic preparation was produced with a yield of 56% and a specific activity of 3730 au per 1 mg protein. the enzyme studied displayed a high thermostability, resistance to metal ions, and performance in a wide ph range and was equal in its properties to foreign analogues. | 2004 | 15029694 |
| [quartz sand as an adsorbent for purification of extracellular glucose oxidase from penicillium funiculosum 46.1]. | the procedure of purification of extracellular glucose oxidase (go, ec 1.1.3.4) from culture-liquid filtrate (clf) of the fungus penicillum funiculosum 46.1 using alluvial quartz sand as an adsorbent has been developed. the modification of sand by changing the charge and polarity did not lead to a significant increase in its adsorption capacity towards go. the effectiveness of sand and aluminum oxide, used as sorbents for isolation of go from clf, was compared. glucose oxidase, isolated from clf ... | 2004 | 15125195 |
| the dual nature of the wheat xylanase protein inhibitor xip-i: structural basis for the inhibition of family 10 and family 11 xylanases. | the xylanase inhibitor protein i (xip-i) from wheat triticum aestivum is the prototype of a novel class of cereal protein inhibitors that inhibit fungal xylanases belonging to glycoside hydrolase families 10 (gh10) and 11 (gh11). the crystal structures of xip-i in complex with aspergillus nidulans (gh10) and penicillium funiculosum (gh11) xylanases have been solved at 1.7 and 2.5 a resolution, respectively. the inhibition strategy is novel because xip-i possesses two independent enzyme-binding s ... | 2004 | 15181003 |
| [growth characteristics and glucose oxidase production by mutant penicillium funiculosum strains]. | the main parameters of growth and glucose oxidase production by the mutant penicillium funiculosum strains bim f-15.3, nmm95.132, and 46.1 were studied. the synthesis of extracellular glucose oxidase by these strains was constitutive and occurred following the phase of exponential growth. the mutant strains also synthesized extracellular invertase and cell-associated catalase and glucose oxidase. the syntheses of invertase, the cell-associated enzymes, and extracellular glucose oxidase were foun ... | 2004 | 15315227 |
| the inhibition specificity of recombinant penicillium funiculosum xylanase b towards wheat proteinaceous inhibitors. | the filamentous fungus penicillium funiculosum produces a mixture of modular and non-modular xylanases belonging to different glycoside hydrolase (gh) families. in the present study, we heterologously expressed the cdna encoding gh11 xylanase b (xynb) and studied the enzymatic properties of the recombinant enzyme. expression in escherichia coli led to the partial purification of a glutathione fusion protein from the soluble fraction whereas the recombinant protein produced in pichia pastoris was ... | 2004 | 15450181 |
| in vitro inhibition of helicobacter pylori by micromycetes. | the anti-helicobacter pylori effect of 22 micromycetes were studied against one standard strain and 11 clinical isolates of h. pylori. penicillium ochlochloron and penicillium funiculosum have been proven the most active fungi against this microorganism. further bio-guided chemical analysis of p. funiculosum afforded an active component identified as (-) 2,3,4-trihydroxybutanamide. | 2005 | 15985225 |
| effect of inoculation techniques and relative humidity on the growth of molds on the surfaces of yellow layer cakes. | four inoculation techniques were compared for initiation of growth on cake surfaces: spot, air cabinet, spray (atomizer), and talc addition methods. molds were isolated from commercial cakes and were identified as aspergillus sydowii, aspergillus ochraceus, penicillium funiculosum, and eurotium herbariorum. cake surfaces were inoculated with mold spores and incubated under three equilibrium relative humidity (erh) levels: 97, 85, and 75%. random contamination by spores in a ventilated air cabine ... | 1998 | 16349479 |
| utilization of methylthio-s-triazine for growth of soil fungi. | aspergillus niger van tieghem, aspergillus tamarii kita, and aspergillus flavus link ex fries utilized the methylthio moiety of 2,4-bis(isopropylamino) -6-methyl-mercapto-s-triazine (prometryne) as a sulfur nutrient source. other soil fungal isolates not affected by prometryne concentrations to 1 mg/ml culture included: aspergillus oryzae (ahlburg) cohn, curvularia lunata (wakker) boedijn, trichoderma viride persoon ex fries, alternaria tenuis nees ex corda, penicillium funiculosum thom, and pae ... | 1970 | 16349873 |
| the crystal structure of polyhydroxybutyrate depolymerase from penicillium funiculosum provides insights into the recognition and degradation of biopolyesters. | polyhydroxybutyrate is a microbial polyester that can be produced from renewable resources, and is degraded by the enzyme polyhydroxybutyrate depolymerase. the crystal structures of polyhydroxybutyrate depolymerase from penicillium funiculosum and its s39 a mutant complexed with the methyl ester of a trimer substrate of (r)-3-hydroxybutyrate have been determined at resolutions of 1.71 a and 1.66 a, respectively. the enzyme is comprised of a single domain, which represents a circularly permuted v ... | 2006 | 16405909 |
| micromycetes, producers of toxins, detected on stored vegetables. | in 2003-2004, investigations of mycological contamination of stored and newly harvested vegetables were carried out. the aim of the study was to detect fungal species able to synthesize toxic metabolites, which are spread on vegetables under various conditions. for mycological investigations, samples of carrots, onions and cabbage were taken from storehouses with different storage periods and conditions. penicillium expansum, p. nalgiovense, mucor silvaticus and penicillium verrucosum were more ... | 2005 | 16457482 |
| purification and properties of an extracellular endo-1,4-beta-xylanase from penicillium citrinum and characterization of the encoding gene. | an extracellular endo-1,4-beta-xylanase was purified from the culture filtrate of a filamentous fungus penicillium citrinum ferm p-15944 grown on birch-wood xylan. the purified enzyme showed a single band on sds-page with an apparent m(r) of 20,000 and had an isoelectric point below 3.5. xylanase activity was optimal at ph 5.0 and 55 degrees c. the genomic dna and cdnas encoding this protein were cloned and sequenced. southern blot analysis indicated that the xylanase gene (xyna) was present as ... | 2005 | 16473771 |
| [immobilizalion of extracellular glucose oxidase from penicillium funiculosum 46.1 on gels of aluminum or zinc hydroxides]. | different methods of immobilization of extracellular glucose oxidase (go) from penicillium funiculosum 46.1 on gels of aluminum or zinc hydroxides have been compared. go from the culture liquid filtrate (clf) associated with zn(oh)2 but not al(oh)3 gels. preparation of samples of immobilized go does not require isolation of the enzyme (clf may be used). go immobilized on zn(oh)2 gels from clf was 1.6 times more efficient in catalyzing d-glucose oxidation than the enzyme contained in the original ... | 2006 | 16761567 |
| [cooxidation of phenol and 4-aminoantipyrin, catalyzed by polymers and copolymers of horseradish root peroxidase and penicillium funiculosum 46.1 glucose oxidase]. | polymers and copolymers of horseradish root peroxidase (hrp) and penicillium funiculosum 46.1 glucose oxidase (go) have been synthesized and their catalytic properties have been characterized (free and immobilized forms of each enzyme were studied). the cooxidation reaction of phenol and 4-aminoantipyrin (4-aap), performed in an aqueous medium in the presence of equimolar amounts of go and hrp, was characterized by effective k(m) and k(cat) of 0.58 mm and 20.9 s(-1) (for phenol), and 14.6 mm and ... | 2006 | 17022456 |
| pcr-rflp of its rdna for the rapid identification of penicillium subgenus biverticillium species. | rflp of its rdna is proposed as a useful tool for molecular identification of the most common species of biverticillate penicillia. 60 isolates were analysed representing 13 species and 21 unique sequences were produced. the combination of five restriction enzymes was successful in separating 12 species. however, the variety penicillium purpurogenum var. rubrisclerotium remained indistinguishable from penicillium funiculosum. p. funiculosum appeared as the most confused species, being mis-identi ... | 2006 | 17196020 |
| substrate and product hydrolysis specificity in family 11 glycoside hydrolases: an analysis of penicillium funiculosum and penicillium griseofulvum xylanases. | two genes encoding family 11 endo-(1,4)-beta-xylanases from penicillium griseofulvum (pgxyna) and penicillium funiculosum (pfxync) were heterologously expressed in escherichia coli as glutathione s-transferase fusion proteins, and the recombinant enzymes were purified after affinity chromatography and proteolysis. pgxyna and pfxync were identical to their native counterparts in terms of molecular mass, pi, n-terminal sequence, optimum ph, and enzymatic activity towards arabinoxylan. further inve ... | 2007 | 17216454 |
| adsorption and hydrolysis reactions of poly(hydroxybutyric acid) depolymerases secreted from ralstonia pickettii t1 and penicillium funiculosum onto poly[(r)-3-hydroxybutyric acid]. | reaction processes of poly[(r)-3-hydroxybutyric acid] (p(3hb)) with two types of poly(hydroxybutyric acid) (phb) depolymerases secreted from ralstonia pickettii t1 and penicillium funiculosum were characterized by means of atomic force microscopy (afm) and quartz crystal microbalance (qcm). the phb depolymerase from r. pickettii t1 consists of catalytic, linker, and substrate-binding domains, whereas the one from p. funiculosum lacks a substrate-binding domain. we succeeded in observing the adso ... | 2007 | 17547455 |
| molecular cloning, and characterization of a modular acetyl xylan esterase from the edible straw mushroom volvariella volvacea. | a new volvariella volvacea gene encoding an acetyl xylan esterase (designated as vvaxe1) was cloned and expressed in pichia pastoris. the cdna contained an orf of 1047 bp encoding 349 amino acids with a calculated mass of 39 990 da. vvaxe1 is a modular enzyme consisting of an n-terminal signal peptide, a catalytic domain, and a cellulose-binding domain. the amino acid sequence of the enzyme exhibited a high degree of similarity to cinnamoyl esterase b from penicillium funiculosum, and acetyl xyl ... | 2007 | 17623028 |
| fungal growth inhibitory properties of new phytosphingolipid analogues. | to study the growth inhibitory properties of a series of phytosphingosine (phs) and phytoceramide (phc) analogues. | 2008 | 18005346 |
| characterization of selected cellulolytic activities of multi-enzymatic complex system from penicillium funiculosum. | the presence of endo-1,4-beta-d-glucanase, cellobiohydrolase, and beta-glucosidase activities in a multi-enzymatic complex system from penicillium funiculosum was investigated. the interesting feature of these enzymes is their synergistic action for the hydrolysis of the native cellulose into glucose units. both endo-1,4-beta-d-glucanase and cellobiohydrolase showed broader ph activity profiles, with ph optima of 4.0 and 4.0-5.0, respectively. however, beta-glucosidase activity showed a narrow p ... | 2008 | 18177005 |
| structure-based mutagenesis of penicillium griseofulvum xylanase using computational design. | penicillium griseofulvum xylanase (pgxyna) belongs to family 11 glycoside hydrolase. it exhibits unique amino acid features but its three-dimensional structure is not known. based upon the x-ray structure of penicillium funiculosum xylanase (pfxync), we generated a three-dimensional model of pgxyna by homology modeling. the native structure of pgxyna displayed the overall beta-jelly roll folding common to family 11 xylanases with two large beta-pleated sheets and a single alpha-helix that form a ... | 2008 | 18384043 |
| heterologous expression of two ferulic acid esterases from penicillium funiculosum. | two recombinant ferulic acid esterases from penicillium funiculosum produced in aspergillus awamori were evaluated for their ability to improve the digestibility of pretreated corn stover. the genes, faea and faeb, were cloned from p. funiculosum and expressed in a. awamori using their native signal sequences. both enzymes contain a catalytic domain connected to a family 1 carbohydrate-binding module by a threonine-rich linker peptide. interestingly, the carbohydrate binding-module is n-terminal ... | 2008 | 18421589 |
| simultaneous saccharification and fermentation of cellulose to ethanol using penicillium funiculosum cellulase and free or immobilized saccharomyces uvarum cells. | no. abstract. | 1983 | 18551453 |
| effect of pretreatment on the hydrolysis of cellulose by penicillium funiculosum cellulase and recovery of enzyme. | penicillium funiculosum produces a complete cellulase which brings about 97% hydrolysis of cotton and has high beta-glucosidase, xylanase, laminarinase, and lichenase activities. this article deals with the effect of different pretreatments on the hydrolysis of sugarcane bagasse by p. funiculosum enzymes and the recovery of enzyme from the insoluble residues. enzymic saccharification of bagasse pretreated with hot 1n naoh followed by neutralization with hci and steam treated under pressure (7 kg ... | 1983 | 18551487 |
| hydrolysis of lignocelluloses by penicillium funiculosum cellulase. | | 1984 | 18553304 |