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[polysaccharide-containing complexes of malleomyces pseudomallei]. 19685733582
the control of experimental infection in mice produced by pseudomonas pseudomallei. 19695787609
melioidosis in malaysia. i. a method for isolation of pseudomonas pseudomallei from soil and surface water. 19695810796
melioidosis in malaysia. ii. distribution of pseudomonas pseudomallei in soil and surface water. 19695810797
melioidosis in malaysia. 3. antibodies to pseudomonas pseudomallei in the human population. 19695817889
osteomyelitis due to pseudomonas pseudomallei. 19665952299
a comparative study of pseudomonas pseudomallei and bacillus mallei. 19665962362
isolation of pseudomonas pseudomallei from an aborted goat foetus. 19666007974
septic arthritis due to pseudomonas pseudomallei. 19676057646
[identification of pseudomonas pseudomallei]. 19836194365
[nature of pseudomonas pseudomallei antigens common to some species of microorganisms].an antigen, common for the infective agents of glanders , tularemia, plague, pseudotuberculosis, cholera and brucellosis, has been obtained from the aqueous saline extract of p. pseudomallei by means of affinity chromatography on antibody sorbent. the isolated antigen has proved to be of a protein-polysaccharide-lipid nature, to have insignificant cathode mobility and to constitute a component of o-antigen. it has also been found to be antigenically related to the tissues of melioidosis-sensitiv ...19846203267
[biological effect of d,l-ureidosuccinic acid dihydrazide on pseudomonas pseudomallei and salmonella typhimurium]. 19826293275
biological effect of d,l-ureidosuccinic acid dihydrazide on pseudomonas pseudomallei and salmonella typhimurium. 19826293407
successful treatment of melioidosis caused by a multiresistant strain in an immunocompromised host with third generation cephalosporins.a 32-yr-old woman with active systemic lupus erythematosus receiving prednisone and azathioprine developed lung abscesses of the right lower lobe caused by pseudomonas pseudomallei, which was resistant concomitantly to chloramphenicol, co-trimoxazole, and tetracycline, but highly sensitive to a new cephalosporin, ceftazidime. melioidosis was treated successfully with lobectomy and parenteral ceftazidime, which was given for 2 months without major side effects. ceftazidime, being bactericidal, ma ...19836342484
[drug sensitivity of pseudomonas pseudomallei]. 19836351503
evaluation of the api 20e and microbact 24e systems for the identification of pseudomonas pseudomallei.one-hundred isolates of pseudomonas pseudomallei were used to evaluate the api 20e and microbact 24e rapid identification systems. the api 20e system identified 50% of the isolates using the revised 1979 manual only, and 63% when referral was made to the computer centre. a higher identification rate (69 and 87%, respectively) was achieved with a longer incubation period of 96 h. the microbact 24e system identified 84% of the isolates as p. pseudomallei using the revised 1983 manual, and 100% whe ...19836362175
biological effect of l-asparagine acid hydrazide on pseudomonas pseudomallei and salmonella typhimurium. 19836370195
[diagnosis of the whitmore bacillus in the laboratory].after realization of a new synthetic medium for whitmore bacillus enrichment and isolation, the authors describe a new specific and immunologic process for pseudomonas pseudomallei isolation from very polluted samples.19836370480
[relative biological effect of l-aspartic acid hydrazide on pseudomonas pseudomallei and salmonella typhimurium]. 19846377831
[the nature of changes in the antigenic spectrum of the causative agent of melioidosis during animal passage].the comparative analysis of the antigenic spectra of pseudomonas pseudomallei museum and subcultured strains, carried out by the method of immunoelectrophoresis, has revealed that, along with an essential increase in the virulence of p. pseudomallei for white mice and changes in the morphology of colonies, a decrease in the amount of detected precipitinogens occurs in the process of subculturing. the immunoelectrophoregrams of the subcultured variants show the absence of antigens 5, 6 and the si ...19836415970
[a study of the possibility of conjugated transmission of the rp4 plasmid from pseudomonas aeruginosa by strains of pseudomonas mallei and pseudomonas pseudomallei]. 19836443857
first report of septicaemic melioidosis in hong kong.septicaemia due to pseudomonas pseudomallei was found in five patients; it complicated pneumonia in three. four patients were old and had diabetes mellitus; the fifth patient, though young, was immunocompromised by steroid therapy. the clinical features were indistinguishable from those of other gram-negative septicaemia. four of the patients died. this was the first report of septicaemic melioidosis in hong kong where the disease might be endemic. soil sampling and serological survey gave furth ...19846485053
in vitro activity of various cephalosporins against pseudomonas pseudomallei. 19846491381
pleuropulmonary melioidosis in a cambodian refugee.a 47-year-old cambodian refugee presented with an acute respiratory illness that featured consolidation of the lower lobe of the left lung and progressive involvement of the adjacent pleura caused by pseudomonas pseudomallei. initial difficulty in identifying the organism resulted in an inadequate duration of therapy. chronic pleural disease followed, and the organism became resistant to many antibiotics during therapy. a diagnosis of pleuropulmonary melioidosis should be entertained and the mic ...19846498689
melioidosis and bilateral third-nerve palsies.central nervous system involvement in melioidosis is unusual. we describe a 34-year-old man who developed a pseudomonas pseudomallei meningitis, manifested as bilateral third-nerve palsies, 13 years after having been in southeast asia. diagnosis was established by a fourfold rise in the serum antibody titer for the bacterium. recovery occurred after treatment with rifampin, isoniazid hydrochloride, ethambutol, and trimethoprim-sulfamethoxazole. since a long latent period from exposure to overt i ...19846537830
[use of transformation for the chromosome mapping of pseudomonas pseudomallei].the mapping of the chromosome sections in p. pseudomallei, responsible for the synthesis of histidine, has been carried out with the use of two- and three-factor crossing. two new groups of marker linkage have been found: his 522 with ilv 658 and his 37 with asp 37 glu 37. the linkage of some of the existing histidine mutations with locus his 37 has been revealed and the relative position of the markers in this linkage group has been shown.19836573084
[r- and s-forms of pseudomonas pseudomallei. vi. ultrastructural characteristics]. 19836650229
granulomatous orchitis and periorchitis caused by pseudomonas pseudomallei in a goat. 19846710827
melioidosis in far north queensland. a clinical and epidemiological review of twenty cases.during the last 4 years, 20 cases of clinical melioidosis were diagnosed in the geographical area between tully and thursday island. sixteen were diagnosed by culture of pseudomonas pseudomallei, and four by positive serology with appropriate clinical features. most cases occurred during or after a heavy wet season. all patients were adult, and males predominated. farmers and stockmen represented predisposed populations due to their prolonged soil contact. ten patients were white australians, si ...19846731679
the prevalence of human melioidosis in northern queensland.sera from 9,047 individuals from northern queensland were examined for the presence of hemagglutinating antibodies to pseudomonas pseudomallei, the causative agent of melioidosis, and 512 (5.7%) were found to have titers of 1:40 or greater. the distribution of positive reactors in various groups was uneven, and significantly higher prevalences of positive antibody titers were found in the sera from aborigines (7.9-10.6%), torres strait islanders (7.8%), vietnamese refugees (29%) and from persons ...19846731680
[studies of r and s forms of pseudomonas pseudomallei. i. morphology of colonies and virulence for albino mice and guinea pigs]. 19826763458
melioidosis: recrudescence with a strain resistant to multiple antimicrobials.we describe a recent case of melioidosis presenting classically in a young vietnam veteran. the case demonstrates extensive antibiotic resistance in this isolate and failure of ceftriaxone, despite its in vitro efficacy. other multiple antimicrobial regimens also failed. melioidosis remains a concern in patients who have previously lived in endemic areas. a high level of diagnostic suspicion is required, especially in patients with a febrile illness or tuberculosis-like presentations. early dete ...19836870060
epididymo-orchitis due to pseudomonas pseudomallei.we report the first case of epididymo-orchitis caused by pseudomonas pseudomallei. the diagnosis was made by isolation of the organism from testicle pus and urine. treatment was successful with chloramphenicol and co-trimoxazole.19836887385
acute septicemic melioidosis occurring in a child with acute lymphoblastic leukemia.a 13-year old boy with acute lymphoblastic leukemia on chemotherapy developed neutropenia and acute cellulitis progressing to fulminating septicemia due to pseudomonas pseudomallei. septicemic melioidosis should be considered in the differential diagnosis of a febrile illness in children who are susceptible to infections.19806931670
influence of d,l-ureidosuccinic acid dihydrazide on some biological properties of pseudomonas pseudomallei and of salmonella typhimurium.the changes induced in strains of pseudomonas pseudomallei and salmonella typhimurium under the action of d.l-ureidosuccinic acid dihydrazide were characterized. a decrease of the speed of multiplication in vitro and of the oxygen consumption was established. the virulence of the strains was reduced and their susceptibility to phagocytosis in vitro was increased.19807001807
primary gram negative pneumonia in adults in papua new guinea.a retrospective review of gram-negative bacteraemia in papua new guinean adults revealed four cases of primary gram negative pneumonia over a two-year period. patients with primary gram negative pneumonia constituted less than 1% of all pneumonias admitted to the medical ward. pseudomonas pseudomallei, haemophilus influenzae and klebsiella pneumoniae were the aetiologic agents. three of four patients died following a fulminant course. it is concluded that primary gram-negative pneumonia in papua ...19807008428
relationship and significance of specific immunoglobulin m antibody response in clinical and subclinical melioidosis.thirty-six hospitalized patients with circulating antibody to pseudomonas pseudomallei were classified as having either clinical or subclinical melioidosis after full clinical examinations and supplementary radiological and culture investigations. qualitative estimations of immunoglobulin m (igm) antibody response were determined by complement fixation, indirect hemagglutination, and immunofluorescence, and the estimations were correlated with the clinical findings in each patient. an attempt wa ...19817026605
demonstration of human antibodies to pseudomonas pseudomallei by indirect fluorescent antibody staining.a rapid, reliable immunofluorescent assay for human antibodies to pseudomonas pseudomallei was devised. the method was found to be sensitive and specific by inhibition tests and by tests for cross reactivity with antisera and cells of closely related pseudomonads other than pseudomonas mallei. preliminary studies suggest that the technique may be useful as a screening test for the detection of melioidosis and that demonstration of specific igm by immunofluorescence may be of value in differentia ...19817029435
fatal bronchopneumonia in a young orang utan caused by pseudomonas pseudomallei. 19827080412
microbiological and immunological studies in a case of human melioidosis diagnosed in denmark.the first case of melioidosis reported in denmark was in a 64-year-old man 2 weeks after his return from a 3-week visit to kenya. the clinical course was characterized by septicaemia and pseudomonas pseudomallei was isolated from repeated blood cultures, urine and sputum. transient impairment of cellular immunity was observed. during the convalescence a cell-mediated immune response against the pathogen was detected by the lymphocyte blast transformation test. two precipitins against p. pseudoma ...19827163779
[studies of r and s forms of pseudomonas pseudomallei. ii. in vitro susceptibility to phagocytosis by human, guinea pig and chicken peripheral blood leukocytes]. 19827171037
[studies of r and s forms of pseudomonas pseudomallei. sensitivity to bactericidal effect of normal human, guinea pig and chicken sera]. 19827171038
pathological changes in goats experimentally inoculated with pseudomonas pseudomallei.sixteen goats were inoculated subcutaneously or intraperitoneally with cultured pseudomonas pseudomallei isolated from a goat. they showed either acute or chronic phase of disease. when inoculated intraperitoneally, animals suffered from a septicemic illness of short duration accompanied with micro-abscesses scattered widely throughout the body. in animals subcutaneously inoculated no clinical signs were exhibited, but the disease was of rather long duration, and lesions were large and localized ...19827186613
melioidosis in the northern territory of australia.melioidosis is being diagnosed with increasing frequency in the northern part of the northern territory, but the mortality rate remains high in the acute septicaemic form of the disease largely because of associated chronic debilitating illnesses. this paper reviews epidemiological and clinical features of human melioidosis in 37 cases seen between 1960 and 1979. infection with pseudomonas pseudomallei is most often contracted during the wet season by persons who have regular contact with soil o ...19817231282
melioidosis with acute meningoencephalomyelitis in a horse.a case of acute meningoencephalomyelitis caused by infection with pseudomonas pseudomallei is described. clinically there was inability to stand, opisthotonus, facial paralysis and nystagmus, rapidly progressing to violent struggling. gross examination revealed malacia and haemorrhage in the medulla oblongata and adjacent spinal cord. microscopically there were disseminated focal neutrophilic accumulations in affected areas, perivascular cuffing with mononuclear cells and lymphocytes and marked ...19817236143
[antigenic structure of pseudomonas pseudomallei].the antigenic scheme has been proposed on the basis of the immunoelectrophoretic study of aqueous-saline extracts from acetone-dried microbial cells belonging to 29p. pseudomallei strains. the proposed scheme consists of 19 precipitinogens; of these, 13 have been shown to be common to all strains under study, the remaining precipitinogens being variable. all antigens are characterized by their charge and the degree of mobility in the electric field, their molecular weight, thermal resistance and ...19817269904
laboratory-acquired infection with pseudomonas pseudomallei (melioidosis). 19817290119
[ecological aspects of pseudomonas pseudomallei interactions with ixodid ticks]. 19817340413
persistence of pseudomonas pseudomallei in soil. 19817342941
cross-reaction to legionella pneumophila antigen in sera with elevated titers to pseudomonas pseudomallei.a significantly greater incidence (p less than 0.005) of legionella pneumophila microagglutination titers greater than or equal to 32 was found in sera with elevated titers to pseudomonas pseudomallei as compared with sera with negative titers for p. pseudomallei antibodies. the greater incidence of l. pneumophila titers in these sera suggests that l. pneumophila and p. pseudomallei share an antigen. the incidence of l. pneumophila microagglutination titers of greater than or equal to 32 in sera ...19807354127
meliodosis.during the five-month wet season of 1977-1978 in northern queensland, six patients with bacteriologically proven melioidosis were successfully treated at the townsville general hospital, the clinical course and management of each case and laboratory findings are described. factors which predisposed them to infection with pseudomonas pseudomallei were diabetes mellitus, cancer, alcoholism, malnutrition, trauma, and pregnancy. successful treatment of melioidosis relied on prompt laboratory diagnos ...19807393058
pseudomonas pseudomallei in an anopthalmic orbit.a 21-year old man sustained multiple facial fractures and skin lacerations during an automobile accident, with resultant necrosis of the left upper eyelid and exposure of the cornea. bacterial and secondary fungal corneal ulceration and perforation occurred, leading to enucleation. cultures from the noninfected anophthalmic orbit approximately eight weeks after enucleation yielded pseudomonas pseudomallei. this is the second isolate of this domallei. this is the second isolate of this organism i ...19807396773
[postinfection immunity in melioidosis].experiments on the reinfection of laboratory animals with melioidosis revealed that guinea-pigs and white mice developed pronounced resistance to the disease, accompanied by the objective [corrected] signs of immunologic transformation (allergization, the presence of specific humoral antibodies, and increase in the phagocytic activity of the cells of the macrophage system). in golden hamsters, highly sensitive to infection with pseudomonas pseudomallei culture, the process of the "all or nothing ...19807405455
septic arthritis of the sternomanubrial joint due to pseudomonas pseudomallei. 19807417354
genito-urinary infections with pseudomonas pseudomallei in australian aboriginals. 19807434413
melioidosis in a galah (cacatua roseicapilla).a second case of a natural infection of melioidosis in a native bird is described. pseudomonas pseudomallei was isolated in pure culture from the liver, spleen and intestinal contents of a galah (cacatua roseicapilla). the case was characterised by focal granuloma formation, often associated with necrosis, in the brain, lungs, liver, spleen and kidneys.19807436921
glucan-induced enhancement of host resistance to selected infectious diseases.we conducted studies with mice, rats, and monkeys which demonstrated the ability of glucan to induce either nonspecific or specific enhancement of host resistance to infectious diseases. intravenous pretreatment of mice with glucan significantly enhanced the survival of mice challenged with either venezuelan equine encephalomyelitis (vee) virus or rift valley fever virus. pretreatment was beneficial when initiated 3 days before challenge with vee virus and 7 days before challenge with rift valle ...19807439978
imaging patterns in melioidosis.melioidosis is an infectious disease caused by pseudomonas pseudomallei. it is seldom diagnosed promptly and, if untreated, can lead to an 80-100% mortality rate. twenty-eight patients with melioidosis were identified over a 6 year period, and their imaging patterns were analysed. respiratory infections were the commonest form of presentation, frequently shown as diffuse airspace consolidation, and accounted for the highest mortality. visceral and musculoskeletal infections were associated with ...19957487762
pilot study of exposure to pseudomonas pseudomallei in northern vietnam. 19937504340
virulence and susceptibility to phagocytosis of pseudomonas pseudomallei r- and s-forms for ground squirrels (citellus citellus l.).virulence and susceptibility to phagocytosis of pseudomonas pseudomallei r- and s-forms for ground squirrels were studied. the alveolar macrophages and blood leucocytes (polymorphonuclear and mononuclear) was used. s-forms survived and multiplied intracellularly more actively in alveolar macrophages than r-forms and their virulence was considerably higher.19937506861
burkholderia pseudomallei and melioidosis: be aware in temperate area. 19937507550
susceptibility of pseudomonas pseudomallei to some newer beta-lactam antibiotics and antibiotic combinations using time-kill studies. 19947512547
the 1990-1991 outbreak of melioidosis in the northern territory of australia: epidemiology and environmental studies.from november 1990 to june 1991 33 acute cases of melioidosis occurred in the northern territory, australia; 25 cases were reported in the capital city, darwin. we carried out an epidemiological investigation to exclude a common source outbreak, describe the risk factors for disease, and develop and institute appropriate control measures. we compared population based attack rates among various risk groups using logistic regression, and the demographic, medical and behavioral risk factors for mel ...19937512752
isolation and characterization of pseudomonas pseudomallei flagellin proteins.flagellin proteins from several different strains of pseudomonas pseudomallei have been isolated and purified to homogeneity by mechanical shearing and differential centrifugation techniques. analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis yielded flagellin monomer protein bands with an estimated m(r) of 43,400. no lipopolysaccharide contamination of the purified protein preparations was detectable by silver staining of flagellin displayed on polyacrylamide gels and by west ...19947513308
neutralizing monoclonal antibodies to an extracellular pseudomonas cepacia protease.pseudomonas cepacia produces at least two extracellular proteases with apparent molecular masses of 36,000 and 40,000 da. the 36-kda protease has high proteolytic activity and the 40-kda protease has low proteolytic activity with hide powder azure as a substrate. monoclonal antibodies (mabs) were raised against the purified 36- and 40-kda proteases. several mabs directed against the 36-kda protease were found to recognize the 40-kda protease by western immunoblot analysis. similarly, a mab direc ...19947516312
isolation and characterization of the outer-membrane proteins of burkholderia (pseudomonas) pseudomallei.membranes obtained from whole-cell lysates of burkholderia (pseudomonas) pseudomallei (strain 319a) were separated into four fractions by sucrose density gradient centrifugation. membranes were characterized by enzymic and chemical analyses, and by sds-page. cytoplasmic membranes and two forms of outer membranes (om-1, om-2) were detected. the major outer-membrane proteins had m(r) values of 70,000, 38,000, 31,000, 24,000 and 17,000. to determine which outer-membrane proteins were common to b. p ...19947516793
construction of a specific dna probe for diagnosis of melioidosis and use as an epidemiological marker of pseudomonas pseudomallei.pseudomonas pseudomallei is a causative agent of melioidosis. the disease manifestations range from fulminant sepsis to asymptomatic seroconversion. in septicemic cases, a mortality rate of 80-90% is reported. rapid and specific diagnosis has become important to the clinical microbiology laboratory. we have developed a p. pseudomallei-specific dna probe. the cloned fragment, herein designated pkku-s23l, contained 1.5 kb of p. pseudomallei chromosomal dna. a radioactively labelled pkku-s23l inser ...19947518036
melioidosis survey in kenya. 19947518617
detection of pseudomonas pseudomallei by pcr and hybridization.a molecular method for the detection of pseudomonas pseudomallei was developed on the basis of the differences in the 23s rrna sequences of related species of the genus pseudomonas. an 18-base oligonucleotide probe, designed following partial sequencing of 23s ribosomal dna (rdna), was used for the identification and detection of p. pseudomallei either by hybridization or by direct pcr. optimal detection was obtained by hybridization of the probe with pcr-amplified rdna rather than with total ge ...19947519629
application of indirect immunofluorescence microscopy to colony identification of pseudomonas pseudomallei.indirect immunofluorescence microscopy was used as a colony identification method of pseudomonas pseudomallei isolates. the antisera against lipopolysaccharide and protein fractions of p. pseudomallei were prepared in guinea pigs and rabbits. with these antisera and fluorescence-labelled anti-guinea pig igg and anti-rabbit igg prepared in sheep (goat), indirect immunofluorescence microscopy was conducted on the colonies of p. pseudomallei and other species of bacteria. the overall results indica ...19937521644
melioidosis in a diabetic sailor.melioidosis was diagnosed in a diabetic sailor who presented with a history and chest radiograph that suggested tuberculosis. melioidosis is a tropical disease with protean manifestations: from asymptomatic infection to chronic cavitary lung disease to overwhelming sepsis. the diagnosis is easily made, even in nonendemic areas when duly considered by the clinicians and microbiology laboratory. ceftazidime has dramatically improved outcomes in hospitalized patients with severe melioidosis.19947521816
pseudomonas pseudomallei isolates collected over 25 years from a non-tropical endemic focus show clonality on the basis of ribotyping.between 1966 and 1991, melioidosis, a disease caused by pseudomonas pseudomallei that is mostly confined to tropical regions, occurred in farm animals and a farmer in temperate south-west western australia. using an escherichia coli probe containing a ribosomal rna operon, p. pseudomallei dna from isolates from 8 animals, a soil sample and the human case showed an identical ribotype on southern blotting. the ribotype was different from the 3 commonest ribotypes seen in tropical australia. this m ...19947523158
[the immunodiagnosis of melioidosis by a solid-phase variant of radioimmunological analysis (ria)].a variant of the solid-phase radioimmunoassay (ria) has been developed for the detection of specific immunoglobulins in laboratory animals infected with pseudomonas pseudomallei. the proposed method has advantages over the indirect hemagglutination test and the enzyme immunoassay in its sensitivity and specificity. the newly developed ria variant, based on group-specific antigens 6 + d, makes it possible to classify the strain causing the disease with the asian or australian serovar of p. pseudo ...19947524250
melioidosis. another etiology of granulomatous osteomyelitis. report of 2 cases.two cases of granulomatous osteomyelitis caused by melioidosis are reported. histologically, the granulomatous lesions were indistinguishable from tuberculosis and required confirmation with microbiological culture. it is important that melioidosis be considered in the differential diagnosis of granulomatous osteomyelitis since the treatment is quite different from tuberculosis.19947525133
interaction of pseudomonas pseudomallei with macrophages. 19947525379
fatal melioidosis in a tourist returning from thailand.severe infections with pseudomonas pseudomallei, the causative agent of melioidosis, is a common cause of community acquired septicaemia in south east asia and parts of northern australia, but infection in travellers returning to the united kingdom is extremely rare. we describe the case of a tourist who acquired melioidosis in thailand. despite intensive intravenous therapy with antibiotics to which the organism was sensitive, the patient's infection proved fatal. ps. pseudomallei was isolated ...19947525733
recent advances in biomedical sciences of burkholderia pseudomallei (basonym: pseudomonas pseudomallei). 19947526019
molecular typing of pseudomonas pseudomallei from imported primates in britain. 19947526531
outbreak of plague-like illness caused by pseudomonas pseudomallei in maharashtra, india. 19947527114
detection of pseudomonas pseudomallei antigen in urine for the diagnosis of melioidosis.an enzyme-linked immunosorbent assay using a fluorescein isothiocyanate (fitc)-anti-fitc amplification system, has been developed to detect pseudomonas pseudomallei antigen in urine. the assay was evaluated in 135 patients with acute melioidosis, 194 hospitalized patients with other disorders, and 40 healthy controls. antigen was detected in the urine of 123 (91%) patients with melioidosis. urinary antigen was found in 85 (96%) of 89 patients with septicemic melioidosis, all six patients with p. ...19947527187
[identification of the bacterium pseudomonas mallei using pseudomonas pseudomallei bacteriophages].phage production by pseudomonas pseudomallei and pseudomonas mallei strains has been studied. 32 p. pseudomallei bacteriophages have been isolated. their spectrum of lytic action against p. pseudomallei, p. mallei and other pseudomonas sp. has been defined. it has been shown that p. pseudomallei bacteriophages pp19, pp23, pp33 may be used for identification p. mallei among related pseudomonas.19947527479
purification and characterization of a protease from pseudomonas pseudomallei.pseudomonas pseudomallei is the causative agent of melioidosis, a glanders-like disease of humans and animals. the pathogenesis of melioidosis is not well understood, and the role of various extracellular enzymes produced by p. pseudomallei in the development of this disease is not known. the present studies were designed to purify and characterize an extracellular protease produced by p. pseudomallei isolates and to test the hypothesis that this protease may play a role in melioidosis. the prot ...19947528633
plague in india. 19957529862
effects of tunicamycin on the ph-activity pattern of acid phosphatase in pseudomonas pseudomallei.the liquid culture of pseudomonas pseudomallei shows a complex feature in in the ph-activity pattern of acid phosphatase, not a single peak curve. there was an evident tendency that the higher activity shifted to the higher ph range with the growth of culture. the culture in the presence of tunicamycin (20 micrograms/ml) showed a decreased activity selectively in the higher ph range, while the activity in the lower ph was more heat-labile. the bacterial cells grown on agar plates containing tuni ...19947529943
[pulmonary melioidosis. report of 4 cases and literature reviewed].in this report, we present 4 patients with pulmonary melioidosis. the literatures on epidemiology, clinical manifestation, roentogenology, diagnosis and therapeutics of melioidosis are reviewed.19947530604
[epidemiology of melioidosis in china].from 1975 to 1989, a total of 73 strains of p. pseudomallei was isolated from the water samples and the pathological samples of human and domestic animals in 13 counties and cities located different latitude from four provinces qiong, yue, gui and xiang in china. serological investigation demonstrated that the geographical distribution of the organism had a significant correlation with the positive rate of antibodies against p. pseudomallei and the native foci of the organisms distributed over t ...19947532109
monoclonal antibodies to pseudomonas pseudomallei and their potential for diagnosis of melioidosis.monoclonal antibodies (mabs) specific for pseudomonas pseudomallei antigens were produced by immunizing balb/c mice with a crude whole cell extract. hybrids secreting mabs specific for p. pseudomallei antigens were identified by an indirect enzyme-linked immunosorbent assay (elisa) against a panel of crude whole cell extracts from p. pseudomallei, p. cepacia, p. aeruginosa, p. putida, p. alcaligenes, xanthomonas maltophilia, klebsiella pneumoniae, escherichia coli, enterobacter cloacae, salmonel ...19957535014
latex agglutination for rapid detection of pseudomonas pseudomallei antigen in urine of patients with melioidosis.a latex agglutination test for the detection of pseudomonas pseudomallei antigen in urine was evaluated for the rapid diagnosis of melioidosis. with unconcentrated urine, antigen was detected in only 18% of patients with melioidosis overall. however, when urine was concentrated 100-fold, antigen was detected in 47% overall and in 67% of patients with septicaemia or disseminated infection, in whom a rapid diagnosis is most important. the specificity of the test was 100%. these results compared fa ...19957538150
selective broths for the isolation of pseudomonas pseudomallei from clinical samples. 19957538232
isolation of pseudomonas pseudomallei from soil in north-eastern thailand.in order to optimize the recovery from soil of pseudomonas pseudomallei, the cause of melioidosis, 3 selective broths were compared. a basal salt solution containing l-threonine (tbss) performed significantly better than trypticase soy broth containing crystal violet and colistin 50 mg/l (cvc50), both in isolation rate and suppression of overgrowth of other organisms, but the addition of colistin to tbss gave the best results overall. in a survey in north-eastern thailand, p. pseudomallei was re ...19957538233
[transport medium for pathogenic pseudomonads].a transporting medium was designed for the transportation and preservation for 5 to 7 days of material infected with agents of malleus and melioidosis. foreign contaminating microorganisms gradually die in this medium, whereas solitary cells of malleus and melioidosis agents (100 bacterial cells per 0.1 ml) survive.19957538858
[differentiation between glanders and melioidosis]. 19957538860
effect of sorbitan monolaurate polyoxyalkylene (tween 20) on the ultrastructure of some bacteria.the ultrastructure of listeria monocytogenes, salmonella typhimurium, pseudomonas pseudomallei and pseudomonas aeruginosa treated with 0.5 and 1% tween 20 was studied by means of transmission and scanning electron microscopy. specific changes in the cell wall and cytoplasmic membrane were observed.19947539732
separation of antigenic glycoprotein fractions from cell-free homogenate of pseudomonas pseudomallei and characterization as tyrosine phosphatase.cell-free extracts were prepared from pseudomonas pseudomallei cells by freezing-thawing, sonication, and differential ultracentrifugation. the extracts were subjected to column chromatography with deae-sepharose to obtain glycoprotein fractions. the fractions showed acid phosphatase activity to p-nitrophenyl phosphate, tyrosine phosphate, serine phosphate, but not to threonine phosphate. they were highly antigenic when tested by immunofluorescence assay with the sera of melioidosis patients.19947539939
cellular lipid and fatty acid compositions of burkholderia pseudomallei strains isolated from human and environment in viet nam.viet nam is known as an endemic area of melioidosis but its etiologic agent originated in viet nam was not extensively studied. for the first time, we analyzed the cellular lipid and fatty acid compositions of 15 vietnamese isolates of burkholderia pseudomallei, 10 from humans and 5 from the environment. cellular lipid compositions were analyzed by two-dimensional thin-layer chromatography on silica gel g plates. cellular fatty acid methyl esters were analyzed by gas chromatography (gc) and gas ...19957540251
[pseudomonas mallei and pseudomonas pseudomallei: introduction and maintenance of natural and recombinant plasmid replicons].comparative analysis of recipient activity of pseudomonas mallei, pseudomonas pseudomallei, and pseudomonas cepacia strains towards naturally occurring and recombinant plasmid replicons was carried out. autonomic broad host range vector plasmids based on rsf1010(incq) and psa(incw) replicons as well as integrative vectors based on psup202(co1e1) replicon have been constructed. the study has shown that naturally occurring plasmids rsf1010(incq), psa(incw), r15(incn), and rp4(incp) are being effic ...19957541512
[laboratory control of effectiveness of chemotherapy in melioidosis].an undoubtable role of the melioidosis germ persistence in the form of l-variants with the development of dth in the relapses of the disease was stated. serological reactions such as cft and phar were shown to be of the diagnostic value but it was by far lower than the allergotest. no correlation between the antibody titers in the tested sera and the isolation of the bacterial or l-variants of the melioidosis germ was observed. the criteria of the recovery from melioidosis were: stable normaliza ...19957541620
rapd analysis of isolates of burkholderia pseudomallei from patients with recurrent melioidosis.twenty-seven isolates of burholderia pseudomallei (formerly pseudomonas pseudomallei) from ten patients with recurrent melioidosis were analysed by rapd. in two cases rapd patterns in recurrent isolates differed from the original isolates; one was considered a likely reinfection while the other may represent relapse from one of two strains initially infecting the patient. in two cases where a change in antibiotic resistance had occurred between original and relapse isolates, slight changes in ra ...19957543859
structural characterization of the lipopolysaccharide o antigens of burkholderia pseudomallei.a serologically typical strain of burkholderia pseudomallei (strain 304b) was found to produce two s-type lipopolysaccharides (lps) differing in the chemical structures of their o-polysaccharide (o-ps) components. structural analysis revealed that one o-antigenic polysaccharide (o-ps i) is an unbranched high-molecular-weight polymer of 1,3-linked 2-o-acetyl-6-deoxy-beta-d-manno-heptopyranose residues. the other lps o antigen (o-ps ii) is an unbranched polymer of repeating disaccharide units havi ...19957543882
pseudomonas pseudomallei-insulin interaction. 19957543888
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