| the use of nanoscale visible light-responsive photocatalyst tio2-pt for the elimination of soil-borne pathogens. | exposure to the soil-borne pathogens burkholderia pseudomallei and burkholderia cenocepacia can lead to severe infections and even mortality. these pathogens exhibit a high resistance to antibiotic treatments. in addition, no licensed vaccine is currently available. a nanoscale platinum-containing titania photocatalyst (tio(2)-pt) has been shown to have a superior visible light-responsive photocatalytic ability to degrade chemical contaminants like nitrogen oxides. the antibacterial activity of ... | 2012 | 22384003 |
| melioidosis from contaminated bore water and successful uv sterilization. | two cases of melioidosis at a residence in rural northern australia were linked to the unchlorinated domestic bore (automated well) water supply, which was found to have a high concentration of burkholderia pseudomallei. using multilocus sequence typing, clinical b. pseudomallei isolates from both cases were identical to an isolate from the bore water supply. a simple uv sterilizer reduced b. pseudomallei from the domestic water supply to undetectable levels. we have shown that uv treatment is h ... | 2013 | 23751401 |
| impaired tlr5 functionality is associated with survival in melioidosis. | melioidosis is infection caused by the flagellated saprophyte burkholderia pseudomallei. tlr5 is a pathogen recognition receptor activated by bacterial flagellin. we studied a genetic variant that encodes a defective tlr5 protein, tlr5(1174c)>t, to elucidate the role of tlr5 in melioidosis. we measured nf-κb activation induced by b. pseudomallei in human embryonic kidney-293 cells transfected with tlr5 and found that b. pseudomallei induced tlr5(1174c)- but not tlr5(1174t)-dependent activation o ... | 2013 | 23447684 |
| nlrc4 and tlr5 each contribute to host defense in respiratory melioidosis. | burkholderia pseudomallei causes the tropical infection melioidosis. pneumonia is a common manifestation of melioidosis and is associated with high mortality. understanding the key elements of host defense is essential to developing new therapeutics for melioidosis. as a flagellated bacterium encoding type iii secretion systems, b. pseudomallei may trigger numerous host pathogen recognition receptors. tlr5 is a flagellin sensor located on the plasma membrane. nlrc4, along with naip proteins, ass ... | 2014 | 25232720 |
| neutrophil elastase causes tissue damage that decreases host tolerance to lung infection with burkholderia species. | two distinct defense strategies can protect the host from infection: resistance is the ability to destroy the infectious agent, and tolerance is the ability to withstand infection by minimizing the negative impact it has on the host's health without directly affecting pathogen burden. burkholderia pseudomallei is a gram-negative bacterium that infects macrophages and causes melioidosis. we have recently shown that inflammasome-triggered pyroptosis and il-18 are equally important for resistance t ... | 2014 | 25166912 |
| type three secretion system-mediated escape of burkholderia pseudomallei into the host cytosol is critical for the activation of nfκb. | burkholderia pseudomallei is the causative agent of melioidosis, a potentially fatal disease endemic in southeast asia and northern australia. this gram-negative pathogen possesses numerous virulence factors including three "injection type" type three secretion systems (t3sss). b. pseudomallei has been shown to activate nfκb in hek293t cells in a toll-like receptor and myd88 independent manner that requires t3ss gene cluster 3 (t3ss3 or t3ssbsa). however, the mechanism of how t3ss3 contributes t ... | 2014 | 24884837 |
| the bacterial aetiology of adult community-acquired pneumonia in asia: a systematic review. | community-acquired pneumonia (cap) is a major cause of adult mortality in asia. appropriate empirical treatment depends on knowledge of the pathogens commonly responsible. however, assessing the aetiological significance of identified organisms is often difficult, particularly with sputum isolates that might represent contamination with oropharyngeal flora. | 2014 | 24781376 |
| development of a prototype lateral flow immunoassay (lfi) for the rapid diagnosis of melioidosis. | burkholderia pseudomallei is a soil-dwelling bacterium and the causative agent of melioidosis. isolation of b. pseudomallei from clinical samples is the "gold standard" for the diagnosis of melioidosis; results can take 3-7 days to produce. alternatively, antibody-based tests have low specificity due to a high percentage of seropositive individuals in endemic areas. there is a clear need to develop a rapid point-of-care antigen detection assay for the diagnosis of melioidosis. previously, we emp ... | 2014 | 24651568 |
| characterization of homologous sphingosine-1-phosphate lyase isoforms in the bacterial pathogen burkholderia pseudomallei. | sphingolipids (sls) are ubiquitous elements in eukaryotic membranes and are also found in some bacterial and viral species. as well as playing an integral structural role, sls also act as potent signaling molecules involved in numerous cellular pathways and have been linked to many human diseases. a central sl signaling molecule is sphingosine-1-phosphate (s1p), whose breakdown is catalyzed by s1p lyase (s1pl), a pyridoxal 5'-phosphate (plp)-dependent enzyme that catalyzes the cleavage of s1p to ... | 2016 | 27784725 |
| physicochemical properties influencing presence of burkholderia pseudomallei in soil from small ruminant farms in peninsular malaysia. | soil is considered to be a major reservoir of burkholderia pseudomallei in the environment. this paper investigates soil physicochemical properties that may influence presence of b. pseudomallei in soil samples from small ruminant farms in peninsular malaysia. soil samples were collected from the farms and cultured for b. pseudomallei. the texture, organic matter and water contents, ph, elemental contents, cation exchange capacities, carbon, sulfur and nitrogen contents were determined. analysis ... | 2016 | 27635652 |
| imported case of melioidosis in oman: case report. | melioidosis is an infectious disease caused by the bacterium burkholderia pseudomallei. it is most commonly described in southeast asia and northern australia and some imported cases in non-endemic areas. we describe the case of a 55-year-old omani man with fulminant sepsis who worked in laos, cambodia. b. pseudomallei was isolated from the patient's blood and was identified by means of microbiological and biochemical tests. we highlight the importance of careful attention to non-fermentative gr ... | 2017 | 28042405 |
| etiologies and resistance profiles of bacterial community-acquired pneumonia in cambodian and neighboring countries' health care settings: a systematic review (1995 to 2012). | community-acquired pneumonia (cap) is one of the most important causes of morbidity and mortality worldwide. etiological data for cambodia is scarce. we aimed to describe the main etiological agents causing cap, and their resistance patterns in cambodia and the greater mekong region. | 2014 | 24626053 |
| characterization of an autotransporter adhesin protein shared by burkholderia mallei and burkholderia pseudomallei. | autotransporters form a large family of outer membrane proteins specifying diverse biological traits of gram-negative bacteria. in this study, we report the identification and characterization of a novel autotransporter gene product of burkholderia mallei (locus tag bma1027 in strain atcc 23344). | 2014 | 24731253 |
| secondary metabolites from bacillus amyloliquefaciens isolated from soil can kill burkholderia pseudomallei. | bacillus species are gram-positive bacteria found in abundance in nature and their secondary metabolites were found to possess various potential activities, notably antimicrobial. in this study, bacillus amyloliquefaciens n2-4 and n3-8 were isolated from soil and their metabolites could kill burkholderia pseudomallei, a gram-negative pathogenic bacterium also found in soil in its endemic areas. moreover, the metabolites were able to kill drug resistant isolates of b. pseudomallei and also inhibi ... | 2017 | 28050857 |
| culturable bacterial microbiota of the stomach of helicobacter pylori positive and negative gastric disease patients. | human stomach is the only known natural habitat of helicobacter pylori (hp), a major bacterial pathogen that causes different gastroduodenal diseases. despite this, the impact of hp on the diversity and the composition of the gastric microbiota has been poorly studied. in this study, we have analyzed the culturable gastric microbiota of 215 malaysian patients, including 131 hp positive and 84 hp negative individuals that were affected by different gastric diseases. non-hp bacteria isolated from ... | 2014 | 25105162 |
| genetic analysis of the cdi pathway from burkholderia pseudomallei 1026b. | contact-dependent growth inhibition (cdi) is a mode of inter-bacterial competition mediated by the cdib/cdia family of two-partner secretion systems. cdia binds to receptors on susceptible target bacteria, then delivers a toxin domain derived from its c-terminus. studies with escherichia coli suggest the existence of multiple cdi growth-inhibition pathways, whereby different systems exploit distinct target-cell proteins to deliver and activate toxins. here, we explore the cdi pathway in burkhold ... | 2015 | 25786241 |
| particle-size dependent effects in the balb/c murine model of inhalational melioidosis. | deposition of burkholderia pseudomallei within either the lungs or nasal passages of the balb/c murine model resulted in different infection kinetics. the infection resulting from the inhalation of b. pseudomallei within a 12 μm particle aerosol was prolonged compared to a 1 μm particle aerosol with a mean time-to-death (mtd) of 174.7 ± 14.9 h and 73.8 ± 11.3 h, respectively. inhalation of b. pseudomallei within 1 μm or 12 μm particle aerosols resulted in a median lethal dose (mld) of 4 and 12 c ... | 2012 | 22919690 |
| immunogenic recombinant burkholderia pseudomallei mpra serine protease elicits protective immunity in mice. | burkholderia pseudomallei is resistant to a diverse group of antimicrobials including third generation cephalosporins whilst quinolones and aminoglycosides have no reliable effect. as therapeutic options are limited, development of more effective forms of immunotherapy is vital to avoid a fatal outcome. in an earlier study, we reported on the b. pseudomallei serine mpra protease, which is relatively stable over a wide ph and temperature range and digests physiological proteins. the present study ... | 2012 | 22919676 |
| structural insights into wcbi, a novel polysaccharide-biosynthesis enzyme. | capsular polysaccharides (cpss) are protective structures on the surfaces of many gram-negative bacteria. the principal cps of the human pathogen and tier 1 select agent burkholderia pseudomallei consists of a linear repeat of --3)--2-o-acetyl-6-deoxy-β-d-manno-heptopyranose-(1-. this cps is critical to the virulence of this emerging pathogen and represents a key target for the development of novel therapeutics. wcbi is one of several genes in the cps biosynthetic cluster whose deletion leads to ... | 2013 | 25075317 |
| pathogens penetrating the central nervous system: infection pathways and the cellular and molecular mechanisms of invasion. | the brain is well protected against microbial invasion by cellular barriers, such as the blood-brain barrier (bbb) and the blood-cerebrospinal fluid barrier (bcsfb). in addition, cells within the central nervous system (cns) are capable of producing an immune response against invading pathogens. nonetheless, a range of pathogenic microbes make their way to the cns, and the resulting infections can cause significant morbidity and mortality. bacteria, amoebae, fungi, and viruses are capable of cns ... | 2014 | 25278572 |
| burkholderia pseudomallei capsular polysaccharide conjugates provide protection against acute melioidosis. | burkholderia pseudomallei, the etiologic agent of melioidosis, is a cdc tier 1 select agent that causes severe disease in both humans and animals. diagnosis and treatment of melioidosis can be challenging, and in the absence of optimal chemotherapeutic intervention, acute disease is frequently fatal. melioidosis is an emerging infectious disease for which there are currently no licensed vaccines. due to the potential malicious use of b. pseudomallei as well as its impact on public health in regi ... | 2014 | 24866807 |
| protection against experimental melioidosis following immunisation with a lipopolysaccharide-protein conjugate. | melioidosis is a severe infectious disease caused by burkholderia pseudomallei. it is refractory to antibiotic treatment and there is currently no licensed vaccine. in this report we detail the construction and protective efficacy of a polysaccharide-protein conjugate composed of b. pseudomallei lipopolysaccharide and the hc fragment of tetanus toxin. immunisation of mice with the lipopolysaccharide-conjugate led to significantly reduced bacterial burdens in the spleen 48 hours after challenge a ... | 2014 | 24892035 |
| a burkholderia pseudomallei outer membrane vesicle vaccine provides protection against lethal sepsis. | the environmental gram-negative encapsulated bacillus burkholderia pseudomallei is the causative agent of melioidosis, a disease associated with high morbidity and mortality rates in areas of southeast asia and northern australia in which the disease is endemic. b. pseudomallei is also classified as a tier i select agent due to the high level of lethality of the bacterium and its innate resistance to antibiotics, as well as the lack of an effective vaccine. gram-negative bacteria, including b. p ... | 2014 | 24671550 |
| burkholderia pseudomallei penetrates the brain via destruction of the olfactory and trigeminal nerves: implications for the pathogenesis of neurological melioidosis. | abstract melioidosis is a potentially fatal disease that is endemic to tropical northern australia and southeast asia, with a mortality rate of 14 to 50%. the bacterium burkholderia pseudomallei is the causative agent which infects numerous parts of the human body, including the brain, which results in the neurological manifestation of melioidosis. the olfactory nerve constitutes a direct conduit from the nasal cavity into the brain, and we have previously reported that b. pseudomallei can colon ... | 2014 | 24736221 |
| enhanced surveillance for fatal dengue-like acute febrile illness in puerto rico, 2010-2012. | dengue is a leading cause of morbidity throughout the tropics; however, accurate population-based estimates of mortality rates are not available. | 2016 | 27727271 |
| molecular evidence of burkholderia pseudomallei genotypes based on geographical distribution. | background. central intermediary metabolism (cim) in bacteria is defined as a set of metabolic biochemical reactions within a cell, which is essential for the cell to survive in response to environmental perturbations. the genes associated with cim are commonly found in both pathogenic and non-pathogenic strains. as these genes are involved in vital metabolic processes of bacteria, we explored the efficiency of the genes in genotypic characterization of burkholderia pseudomallei isolates, compar ... | 2016 | 26998408 |
| burkholderia pseudomallei transcriptional adaptation in macrophages. | burkholderia pseudomallei is a facultative intracellular pathogen of phagocytic and non-phagocytic cells. how the bacterium interacts with host macrophage cells is still not well understood and is critical to appreciate the strategies used by this bacterium to survive and how intracellular survival leads to disease manifestation. | 2012 | 22823543 |
| variable virulence factors in burkholderia pseudomallei (melioidosis) associated with human disease. | burkholderia pseudomallei is a gram-negative environmental bacterium that causes melioidosis, a potentially life-threatening infectious disease affecting mammals, including humans. melioidosis symptoms are both protean and diverse, ranging from mild, localized skin infections to more severe and often fatal presentations including pneumonia, septic shock with multiple internal abscesses and occasionally neurological involvement. several ubiquitous virulence determinants in b. pseudomallei have al ... | 2014 | 24618705 |
| systematic mutagenesis of genes encoding predicted autotransported proteins of burkholderia pseudomallei identifies factors mediating virulence in mice, net intracellular replication and a novel protein conferring serum resistance. | burkholderia pseudomallei is the causative agent of the severe tropical disease melioidosis, which commonly presents as sepsis. the b. pseudomallei k96243 genome encodes eleven predicted autotransporters, a diverse family of secreted and outer membrane proteins often associated with virulence. in a systematic study of these autotransporters, we constructed insertion mutants in each gene predicted to encode an autotransporter and assessed them for three pathogenesis-associated phenotypes: virulen ... | 2015 | 25830295 |
| characterization of new virulence factors involved in the intracellular growth and survival of burkholderia pseudomallei. | burkholderia pseudomallei, the causative agent of melioidosis, has complex and poorly understood extracellular and intracellular lifestyles. we used transposon-directed insertion site sequencing (tradis) to retrospectively analyze a transposon library that had previously been screened through a balb/c mouse model to identify genes important for growth and survival in vivo. this allowed us to identify the insertion sites and phenotypes of negatively selected mutants that were previously overlooke ... | 2016 | 26712202 |
| rapid identification of burkholderia mallei and burkholderia pseudomallei by intact cell matrix-assisted laser desorption/ionisation mass spectrometric typing. | burkholderia (b.) pseudomallei and b. mallei are genetically closely related species. b. pseudomallei causes melioidosis in humans and animals, whereas b. mallei is the causative agent of glanders in equines and rarely also in humans. both agents have been classified by the cdc as priority category b biological agents. rapid identification is crucial, because both agents are intrinsically resistant to many antibiotics. matrix-assisted laser desorption/ionisation mass spectrometry (maldi-tof ms) ... | 2012 | 23046611 |
| rapid detection of burkholderia pseudomallei in blood cultures using a monoclonal antibody-based immunofluorescent assay. | melioidosis is a severe bacterial infection caused by burkholderia pseudomallei. rapid antimicrobial therapy is necessary to improve patient outcome, which is aided by direct detection of b. pseudomallei in clinical samples. a drawback for all antigen assays is that the number of b. pseudomallei in blood usually falls below the achievable level of detection. we performed a prospective cohort study of 461 patients with 541 blood cultures to evaluate the utility of a pre-incubation step prior to d ... | 2013 | 24019434 |
| human melioidosis, malawi, 2011. | a case of human melioidosis caused by a novel sequence type of burkholderia pseudomallei occurred in a child in malawi, southern africa. a literature review showed that human cases reported from the continent have been increasing. | 2013 | 23735189 |
| a retrospective analysis of melioidosis in cambodian children, 2009-2013. | melioidiosis, infection by burkholderia pseudomallei, is an important but frequently under-recognised cause of morbidity and mortality in southeast asia and elsewhere in the tropics. data on the epidemiology of paediatric melioidosis in cambodia are extremely limited. | 2016 | 27871233 |
| a quadruplex real-time pcr assay for the rapid detection and differentiation of the most relevant members of the b. pseudomallei complex: b. mallei, b. pseudomallei, and b. thailandensis. | the burkholderia pseudomallei complex classically consisted of b. mallei, b. pseudomallei, and b. thailandensis, but has now expanded to include b. oklahomensis, b. humptydooensis, and three unassigned burkholderia clades. methods for detecting and differentiating the b. pseudomallei complex has been the topic of recent research due to phenotypic and genotypic similarities of these species. b. mallei and b. pseudomallei are recognized as cdc tier 1 select agents, and are the causative agents of ... | 2016 | 27736903 |
| maldi-tof ms contribution to diagnosis of melioidosis in a nonendemic country in three french travellers. | melioidosis is an endemic disease in southeast asia and northern australia. an increasing number of cases are being reported in nonendemic countries, making the diagnosis less obvious. we discuss the identification of burkholderia pseudomallei using matrix-assisted desorption ionization-time of flight mass spectrometry on the occasion of recent cases of imported melioidosis in french travellers. | 2016 | 27222715 |
| production of interleukin-27 by human neutrophils regulates their function during bacterial infection. | septicemia is the most severe form of melioidosis caused by the gram-negative bacterium, burkholderia pseudomallei. here, we show that levels of il-27p28 transcript and protein were both significantly elevated in patients with sepsis, particularly melioidosis and in patients with unfavorable disease outcome. moreover, human monocytes/macrophages and neutrophils were the major source of il-27 during infection. the addition of exogenous il-27 in vitro resulted in significantly increased bacterial ... | 2012 | 22965735 |
| bee venom (apis mellifera) an effective potential alternative to gentamicin for specific bacteria strains: bee venom an effective potential for bacteria. | mellitine, a major component of bee venom (bv, apis mellifera), is more active against gram positive than gram negative bacteria. moreover, bv has been reported to have multiple effects, including antibacterial, antivirus, and anti-inflammation effects, in various types of cells. in addition, wasp venom has been reported to have antibacterial properties. the aim of this study was to evaluate the antibacterial activity of bv against selected gram positive and gram negative bacterial strains of me ... | 2016 | 27695631 |
| structural basis of toxicity and immunity in contact-dependent growth inhibition (cdi) systems. | contact-dependent growth inhibition (cdi) systems encode polymorphic toxin/immunity proteins that mediate competition between neighboring bacterial cells. we present crystal structures of cdi toxin/immunity complexes from escherichia coli ec869 and burkholderia pseudomallei 1026b. despite sharing little sequence identity, the toxin domains are structurally similar and have homology to endonucleases. the ec869 toxin is a zn(2+)-dependent dnase capable of completely degrading the genomes of target ... | 2012 | 23236156 |
| mapping the aetiology of non-malarial febrile illness in southeast asia through a systematic review--terra incognita impairing treatment policies. | an increasing use of point of care diagnostic tests that exclude malaria, coupled with a declining malaria burden in many endemic countries, is highlighting the lack of ability of many health systems to manage other causes of febrile disease. a lack of knowledge of distribution of these pathogens, and a lack of screening and point-of-care diagnostics to identify them, prevents effective management of these generally treatable contributors to disease burden. while prospective data collection is v ... | 2012 | 22970193 |
| phylogenomic analysis reveals an asian origin for african burkholderia pseudomallei and further supports melioidosis endemicity in africa. | burkholderia pseudomallei, an environmental bacterium that causes the deadly disease melioidosis, is endemic in northern australia and southeast asia. an increasing number of melioidosis cases are being reported in other tropical regions, including africa and the indian ocean islands. b. pseudomallei first emerged in australia, with subsequent rare dissemination event(s) to southeast asia; however, its dispersal to other regions is not yet well understood. we used large-scale comparative genomic ... | 2016 | 27303718 |
| redefining the pf06864 pfam family based on burkholderia pseudomallei pilo2(bp) s-sad crystal structure. | type iv pili are surface-exposed filaments and bacterial virulence factors, represented by the tfpa and tfpb types, which assemble via specific machineries. the tfpb group is further divided into seven variants, linked to heterogeneity in the assembly machineries. here we focus on pilo2(bp), a protein component of the tfpb r64 thin pilus variant assembly machinery from the pathogen burkholderia pseudomallei. pilo2(bp) belongs to the pf06864 pfam family, for which an improved definition is presen ... | 2014 | 24728008 |
| the expanding role of type ii secretion in bacterial pathogenesis and beyond. | type ii secretion (t2s) is one means by which gram-negative pathogens secrete proteins into the extracellular milieu and/or host organisms. based upon recent genome sequencing, it is clear that t2s is largely restricted to the proteobacteria, occurring in many, but not all genera in the α-, β-, γ-, and δ- classes. prominent human and/or animal pathogens that express a t2s system(s) include acinetobacter baumannii, burkholderia pseudomallei, chlamydia trachomatis, escherichia coli, klebsiella pne ... | 2017 | 28264910 |
| a case-control study of community-acquired acinetobacter baumannii pneumonia and melioidosis pneumonia in northeast thailand: an emerging fatal disease with unique clinical features. | acinetobacter baumannii is the emerging cause of severe and often fatal gram-negative, community-acquired pneumonia (cap-ab) in thailand. due to its rarity, its specific clinical features are ill defined. in this retrospective study, we compared the demographic data, risk factors, clinical characteristics, radiographic pattern, and microbiological data between cap-ab and burkholderia pseudomallei cap (cap-bp) to identify the clinical features and risk factors of cap-ab. cap-ab was associated wit ... | 2017 | 27789057 |
| antibiotic resistance in burkholderia species. | the genus burkholderia comprises metabolically diverse and adaptable gram-negative bacteria, which thrive in often adversarial environments. a few members of the genus are prominent opportunistic pathogens. these include burkholderia mallei and burkholderia pseudomallei of the b. pseudomallei complex, which cause glanders and melioidosis, respectively. burkholderia cenocepacia, burkholderia multivorans, and burkholderia vietnamiensis belong to the burkholderia cepacia complex and affect mostly c ... | 2016 | 27620956 |
| gipsy: genomic island prediction software. | bacteria are highly diverse organisms that are able to adapt to a broad range of environments and hosts due to their high genomic plasticity. horizontal gene transfer plays a pivotal role in this genome plasticity and in evolution by leaps through the incorporation of large blocks of genome sequences, ordinarily known as genomic islands (geis). geis may harbor genes encoding virulence, metabolism, antibiotic resistance and symbiosis-related functions, namely pathogenicity islands (pais), metabol ... | 2016 | 26376473 |
| tn5/7-lux: a versatile tool for the identification and capture of promoters in gram-negative bacteria. | the combination of imaging technologies and luciferase-based bioluminescent bacterial reporter strains provide a sensitive and simple non-invasive detection method (photonic bioimaging) for the study of diverse biological processes, as well as efficacy of therapeutic interventions, in live animal models of disease. the engineering of bioluminescent bacteria required for photonic bioimaging is frequently hampered by lack of promoters suitable for strong, yet stable luciferase gene expression. | 2015 | 25648327 |
| plant-based hydrocarbon esters from tragia involucrata possess antimicrobial and anti-inflammatory activities. | antimicrobial and anti-inflammatory activities of hydrocarbon esters obtained from tragia involucrata were evaluated by disk-diffusion (250 µg/ml), and broth-dilution (500-7.8 µg/ml), methods against bacteria. among the compounds, shellsol showed the most potent activity against burkholderia pseudomallei (khw), aeromonas hydrophila, staphylococcus aureus, bacillus subtilis, streptococcus pyogenes, klebsiella pneumoniae, proteus mirabilis, and streptococcus pneumoniae. interestingly, vinyl hexyle ... | 2013 | 23713670 |
| rapid antimicrobial susceptibility testing of bacillus anthracis, yersinia pestis, and burkholderia pseudomallei by use of laser light scattering technology. | rapid methods to determine antimicrobial susceptibility would assist in the timely distribution of effective treatment or postexposure prophylaxis in the aftermath of the release of bacterial biothreat agents such as bacillus anthracis, yersinia pestis, or burkholderia pseudomallei conventional susceptibility tests require 16 to 48 h of incubation, depending on the bacterial species. we evaluated a method that is based on laser light scattering technology that measures cell density in real time. ... | 2016 | 26984973 |
| inactivation of bacterial and viral biothreat agents on metallic copper surfaces. | in recent years several studies in laboratory settings and in hospital environments have demonstrated that surfaces of massive metallic copper have intrinsic antibacterial and antiviral properties. microbes are rapidly inactivated by a quick, sharp shock known as contact killing. the underlying mechanism is not yet fully understood; however, in this process the cytoplasmic membrane is severely damaged. pathogenic bacterial and viral high-consequence species able to evade the host immune system a ... | 2014 | 25100640 |
| construction and characterization of stable, constitutively expressed, chromosomal green and red fluorescent transcriptional fusions in the select agents, bacillus anthracis, yersinia pestis, burkholderia mallei, and burkholderia pseudomallei. | here, we constructed stable, chromosomal, constitutively expressed, green and red fluorescent protein (gfp and rfp) as reporters in the select agents, bacillus anthracis, yersinia pestis, burkholderia mallei, and burkholderia pseudomallei. using bioinformatic approaches and other experimental analyses, we identified p0253 and p1 as potent promoters that drive the optimal expression of fluorescent reporters in single copy in b. anthracis and burkholderia spp. as well as their surrogate strains, r ... | 2014 | 25044501 |
| reverse line blot macroarray for simultaneous detection and characterization of four biological warfare agents. | the need for a rapid detection and characterization of biowarfare (bw) agents cannot be over emphasized. with diverse array of potential bw pathogen available presently, rapid identification of the pathogen is crucial, so that specific therapy and control measures can be initiated. we have developed a multiplex polymerase chain reaction based reverse line blot macroarray to simultaneously detect four pathogens of bw importance viz. bacillus anthracis, yersinia pestis, brucella melitensis and bur ... | 2013 | 24426077 |
| a sensitive & specific multiplex pcr assay for simultaneous detection of bacillus anthracis, yersinia pestis, burkholderia pseudomallei & brucella species. | bacillus anthracis, yersinia pestis, burkholderia pseudomallei and brucella species are potential biowarfare agents. classical bacteriological methods for their identification are cumbersome, time consuming and of potential risk to the handler. | 2013 | 24056564 |
| the role of short-chain dehydrogenase/oxidoreductase, induced by salt stress, on host interaction of b. pseudomallei. | burkholderia pseudomallei is the causative agent of melioidosis, a frequently occurring disease in northeastern thailand, where soil and water high in salt content are common. using microarray analysis, we previously showed that b. pseudomallei up-regulated a short-chain dehydrogenase/oxidoreductase (sdo) under salt stress. however, the importance of sdo in b. pseudomallei infection is unknown. this study aimed to explore the function of b. pseudomallei sdo, and to investigate its role in intera ... | 2014 | 24382268 |
| galleria mellonella as an infection model for select agents. | the use of animal models is a key step to better understand bacterial virulence factors and their roles in host/pathogen interactions. to avoid the ethical and cost problems of mammalian models in bacterial virulence research, several insect models have been developed. one of these models, the larvae of the greater wax moth galleria mellonella, has been shown to be relevant for several fungal and bacterial mammalian pathogens. here, we describe the use g. mellonella to study virulence of the hig ... | 2014 | 25172272 |
| accurate identification of members of the burkholderia cepacia complex in cystic fibrosis sputum. | the burkholderia cepacia complex (bcc) is a group of closely related species which includes opportunistic pathogens causing chronic respiratory infections in immunocompromised patients, or individuals affected by cystic fibrosis (cf). other burkholderia species causing infection in the cf population are burkholderia gladioli and burkholderia pseudomallei. traditional phenotypic analyses have been demonstrated to be inadequate for reliable identifications of isolates of bcc and b. gladioli. a pan ... | 2016 | 26671758 |
| cd4+ t cell epitopes of flic conserved between strains of burkholderia: implications for vaccines against melioidosis and cepacia complex in cystic fibrosis. | burkholderia pseudomallei is the causative agent of melioidosis characterized by pneumonia and fatal septicemia and prevalent in southeast asia. related burkholderia species are strong risk factors of mortality in cystic fibrosis (cf). the b. pseudomallei flagellar protein flic is strongly seroreactive and vaccination protects challenged mice. we assessed b. pseudomallei flic peptide binding affinity to multiple hla class ii alleles and then assessed cd4 t cell immunity in hla class ii transgeni ... | 2014 | 25392525 |
| the in vivo extracellular life of facultative intracellular bacterial parasites: role in pathogenesis. | classically labeled facultative intracellular pathogens are characterized by the ability to have an intracellular phase in the host, which is required for pathogenicity, while capable of extracellular growth in vitro. the ability of these bacteria to replicate in cell-free conditions is usually assessed by culture in artificial bacteriological media. however, the extracellular growth ability of these pathogens may also be expressed by a phase of extracellular infection in the natural setting of ... | 2013 | 22795971 |
| comparison of mast burkholderia cepacia, ashdown + gentamicin, and burkholderia pseudomallei selective agar for the selective growth of burkholderia spp. | reliable identification of pathogenic burkholderia spp. like burkholderia mallei and burkholderia pseudomallei in clinical samples is desirable. three different selective media were assessed for reliability and selectivity with various burkholderia spp. and nontarget organisms. mast burkholderia cepacia agar, ashdown + gentamicin agar, and b. pseudomallei selective agar were compared. a panel of 116 reference strains and well-characterized clinical isolates, comprising 30 b. pseudomallei, 20 b. ... | 2017 | 28386468 |
| the art of persistence-the secrets to burkholderia chronic infections. | the gram-negative proteobacteria genus burkholderia encompasses multiple bacterial species that are pathogenic to humans and other vertebrates. two pathogenic species of interest within this genus are burkholderia pseudomallei (bpm) and the b. cepacia complex (bcc); the former is the causative agent of melioidosis in humans and other mammals, and the latter is associated with pneumonia in immunocompromised patients. one understudied and shared characteristic of these two pathogenic groups is the ... | 2016 | 27440810 |
| choline catabolism in burkholderia thailandensis is regulated by multiple glutamine amidotransferase 1-containing arac family transcriptional regulators. | burkholderia thailandensis is a soil-dwelling bacterium that shares many metabolic pathways with the ecologically similar, but evolutionarily distant, pseudomonas aeruginosa among the diverse nutrients it can utilize is choline, metabolizable to the osmoprotectant glycine betaine and subsequently catabolized as a source of carbon and nitrogen, similar to p. aeruginosa orthologs of genes in the choline catabolic pathway in these two bacteria showed distinct differences in gene arrangement as well ... | 2016 | 27381916 |
| identification of an ompw homologue in burkholderia pseudomallei, a protective vaccine antigen against melioidosis. | burkholderia pseudomallei is the causative agent of melioidosis, which is associated with a range of clinical manifestations, including sepsis and fatal pneumonia and is endemic in southeast asia and northern australia. treatment can be challenging and control of infection involves prolonged antibiotic therapy, yet there are no approved vaccines available to prevent infection. our aim was to develop and assess the potential of a prophylactic vaccine candidate targeted against melioidosis. the id ... | 2016 | 27091689 |
| burkholderia stagnalis sp. nov. and burkholderia territorii sp. nov., two novel burkholderia cepacia complex species from environmental and human sources. | nine burkholderia cepacia complex (bcc) bacteria were isolated during environmental surveys for the ecological niche of burkholderia pseudomallei, the aetiological agent of melioidosis, in the northern territory of australia. they represented two multi-locus sequence analysis-based clusters, referred to as bcc b and bcc l. three additional environmental and clinical bcc b isolates were identified upon deposition of the sequences in the pubmlst database. analysis of the concatenated nucleotide se ... | 2015 | 25872960 |
| gas chromatography-mass spectrometry method for rapid identification and differentiation of burkholderia pseudomallei and burkholderia mallei from each other, burkholderia thailandensis and several members of the burkholderia cepacia complex. | to develop a simple gas chromatography-mass spectrometry (gc-ms) method for the detection and differentiation of burkholderia pseudomallei and burkholderia mallei from each other, burkholderia thailandensis and several members of the burkholderia cepacia complex. | 2013 | 23879736 |
| misidentification of burkholderia pseudomallei as burkholderia cepacia by the vitek 2 system. | a previously healthy chinese male returned from working in the malaysian jungle with a fever. a blood culture grew gram-negative bacilli that were initially identified as burkholderia cepacia by the vitek 2 system but were subsequently found to be burkholderia pseudomallei by partial sequencing of the 16s rrna gene. the identification of b. pseudomallei using commercially available automated systems is problematic and clinicians in non-endemic areas should be aware of the possibility of melioido ... | 2012 | 22820689 |
| development of a multiplex pcr assay for rapid identification of burkholderia pseudomallei, burkholderia thailandensis, burkholderia mallei and burkholderia cepacia complex. | we have developed a multiplex pcr assay for rapid identification and differentiation of cultures for burkholderia pseudomallei, burkholderia thailandensis, burkholderia mallei and burkholderia cepacia complex. the assay is valuable for use in clinical and veterinary laboratories, and in a deployable laboratory during outbreaks. | 2012 | 22705921 |
| burkholderia pseudomallei and burkholderia mallei vaccines: are we close to clinical trials? | b. pseudomallei is the cause of melioidosis, a serious an often fatal disease of humans and animals. the closely related bacterium b. mallei, which cases glanders, is considered to be a clonal derivative of b. pseudomallei. both b. pseudomallei and b. mallei were evaluated by the united states and the former ussr as potential bioweapons. much of the effort to devise biodefence vaccines in the past decade has been directed towards the identification and formulation of sub-unit vaccines which coul ... | 2017 | 28336210 |
| development of immunoassays for burkholderia pseudomallei typical and atypical lipopolysaccharide strain typing. | burkholderia pseudomallei is the causative agent of melioidosis, a severe infection endemic to many tropical regions. lipopolysaccharide (lps) is recognized as an important virulence factor used by b. pseudomallei isolates of b. pseudomallei have been shown to express one of four different types of lps (typical lps, atypical lps types b and b2, and rough lps) and in vitro studies have demonstrated that lps types may impact disease severity. the association between lps types and clinical manifest ... | 2017 | 27994103 |
| characterization of in vitro phenotypes of burkholderia pseudomallei and burkholderia mallei strains potentially associated with persistent infection in mice. | burkholderia pseudomallei (bp) and burkholderia mallei (bm), the agents of melioidosis and glanders, respectively, are tier 1 biothreats. they infect humans and animals, causing disease ranging from acute and fatal to protracted and chronic. chronic infections are especially challenging to treat, and the identification of in vitro phenotypic markers which signal progression from acute to persistent infection would be extremely valuable. first, a phenotyping strategy was developed employing colon ... | 2017 | 27738703 |
| dbsecsys 2.0: a database of burkholderia mallei and burkholderia pseudomallei secretion systems. | burkholderia mallei and b. pseudomallei are the causative agents of glanders and melioidosis, respectively, diseases with high morbidity and mortality rates. b. mallei and b. pseudomallei are closely related genetically; b. mallei evolved from an ancestral strain of b. pseudomallei by genome reduction and adaptation to an obligate intracellular lifestyle. although these two bacteria cause different diseases, they share multiple virulence factors, including bacterial secretion systems, which repr ... | 2016 | 27650316 |
| developing peptide mimotopes of capsular polysaccharides and lipopolysaccharides protective antigens of pathogenic burkholderia bacteria. | burkholderia pseudomallei (bp) and burkholderia mallei (bm) are two species of pathogenic burkholderia bacteria. our laboratory previously identified four monoclonal antibodies (mabs) that reacted against burkholderia capsular polysaccharides (ps) and lipopolysaccharides (lps) and effectively protected against a lethal dose of bp/bm infections in mice. in this study, we used phage display panning against three different phage peptide libraries to select phage clones specifically recognized by ea ... | 2016 | 27328059 |
| glanders and the risk for its introduction through the international movement of horses. | glanders is the contagious zoonotic disease caused by infection with burkholderia mallei. it affects primarily horses, donkeys and mules. the disease was eradicated from large areas of the western world in the early 20th century, but, over the last 10-20 years, has emerged and re-emerged in areas in which it was previously unknown or had been eradicated. although glanders was previously thought to manifest in only acute or chronic presentations, it now appears that b. mallei can produce latent i ... | 2016 | 27288893 |
| melioidosis and glanders modulation of the innate immune system: barriers to current and future vaccine approaches. | burkholderia pseudomallei and burkholderia mallei are pathogenic bacteria causing fatal infections in animals and humans. both organisms are classified as tier 1 select agents owing to their highly fatal nature, potential/prior use as bioweapons, severity of disease via respiratory exposure, intrinsic resistance to antibiotics, and lack of a current vaccine. disease manifestations range from acute septicemia to chronic infection, wherein the facultative intracellular lifestyle of these organisms ... | 2016 | 27010618 |
| characterization of the murine macrophage response to infection with virulent and avirulent burkholderia species. | burkholderia pseudomallei (bp) and burkholderia mallei (bm) are gram-negative facultative intracellular pathogens, which are the causative agents of melioidosis and glanders, respectively. depending on the route of exposure, aerosol or transcutaneous, infection by bp or bm can result in an extensive range of disease - from acute to chronic, relapsing illness to fatal septicemia. both diseases are associated with difficult diagnosis and high fatality rates. about ninety five percent of patients s ... | 2015 | 26545875 |
| a reverse-phase protein microarray-based screen identifies host signaling dynamics upon burkholderia spp. infection. | burkholderia is a diverse genus of gram-negative bacteria that causes high mortality rate in humans, equines and cattle. the lack of effective therapeutic treatments poses serious public health threats. developing insights toward host-burkholderia spp. interaction is critical for understanding the pathogenesis of infection as well as identifying therapeutic targets for drug development. reverse-phase protein microarray technology was previously proven to identify and characterize novel biomarker ... | 2015 | 26284031 |
| serum biomarkers of burkholderia mallei infection elucidated by proteomic imaging of skin and lung abscesses. | the bacterium burkholderia mallei is the etiological agent of glanders, a highly contagious, often fatal zoonotic infectious disease that is also a biodefense concern. clinical laboratory assays that analyze blood or other biological fluids are the highest priority because these specimens can be collected with minimal risk to the patient. however, progress in developing sensitive assays for monitoring b. mallei infection is hampered by a shortage of useful biomarkers. | 2015 | 26034464 |
| recent advances in burkholderia mallei and b. pseudomallei research. | burkholderia mallei and burkholderia pseudomallei are gram-negative organisms, which are etiological agents of glanders and melioidosis, respectively. although only b. pseudomallei is responsible for a significant number of human cases, both organisms are classified as tier 1 select agents and their diseases lack effective diagnosis and treatment. despite a recent resurgence in research pertaining to these organisms, there are still a number of knowledge gaps. this article summarizes the latest ... | 2015 | 25932379 |
| plasticity of the malleobactin pathway and its impact on siderophore action in human pathogenic bacteria. | the human pathogenic bacteria burkholderia mallei, burkholderia pseudomallei, and burkholderia thailandensis harbor a highly conserved gene cluster coding for the biosynthesis of the long sought-after malleobactins. four new, unexpected congeners of the malleobactin family that were isolated and fully characterized in this study feature unusual deviations from the parent, ornibactin-like architecture. thus, the malleobactin non-ribosomal peptide synthetase (nrps) has a rare flexibility that yiel ... | 2015 | 25873483 |
| intracellular bacteria find the right motion. | benanti et al. report that burkholderia pseudomallei and burkholderia mallei bacteria express proteins that mimic ena/vasp family proteins to polymerize actin, thereby inducing actin-based motility. thus, bacteria can use the various cellular actin polymerization mechanisms for intra- and inter-cellular dissemination. | 2015 | 25860603 |
| use of the phytopathogenic effect for studies of burkholderia virulence. | the phytopathogenic effect of the pseudomallei group burkholderia is demonstrated on the peireskia aculeata model. a method for evaluation of the effect is suggested. the effect correlates with the levels of burkholderia pseudomallei, burkholderia mallei, and burkholderia thailandensis virulence for laboratory animals. p. aculeata can be used as a model for preliminary studies of the virulence of the above species. | 2015 | 25705037 |
| oropharyngeal aspiration of burkholderia mallei and burkholderia pseudomallei in balb/c mice. | burkholderia mallei and burkholderia pseudomallei are potentially lethal pathogens categorized as biothreat agents due, in part, to their ability to be disseminated via aerosol. there are no protective vaccines against these pathogens and treatment options are limited and cumbersome. since disease severity is greatest when these agents are inhaled, efforts to develop pre- or post-exposure prophylaxis focus largely on inhalation models of infection. here, we demonstrate a non-invasive and technic ... | 2014 | 25503969 |
| characterization of cellular immune response and innate immune signaling in human and nonhuman primate primary mononuclear cells exposed to burkholderia mallei. | burkholderia pseudomallei infection causes melioidosis and is often characterized by severe sepsis. although rare in humans, burkholderia mallei has caused infections in laboratory workers, and the early innate cellular response to b. mallei in human and nonhuman primates has not been characterized. in this study, we examined the primary cellular immune response to b. mallei in pbmc cultures of non-human primates (nhps), chlorocebus aethiops (african green monkeys), macaca fascicularis (cynomolg ... | 2015 | 25450887 |
| cross-species comparison of the burkholderia pseudomallei, burkholderia thailandensis, and burkholderia mallei quorum-sensing regulons. | burkholderia pseudomallei, burkholderia thailandensis, and burkholderia mallei (the bptm group) are close relatives with very different lifestyles: b. pseudomallei is an opportunistic pathogen, b. thailandensis is a nonpathogenic saprophyte, and b. mallei is a host-restricted pathogen. the acyl-homoserine lactone quorum-sensing (qs) systems of these three species show a high level of conservation. we used transcriptome sequencing (rna-seq) to define the quorum-sensing regulon in each species, an ... | 2014 | 25182491 |
| raman spectroscopic detection and identification of burkholderia mallei and burkholderia pseudomallei in feedstuff. | burkholderia mallei (the etiologic agent of glanders in equines and rarely humans) and burkholderia pseudomallei, causing melioidosis in humans and animals, are designated category b biothreat agents. the intrinsically high resistance of both agents to many antibiotics, their potential use as bioweapons, and their low infectious dose, necessitate the need for rapid and accurate detection methods. current methods to identify these organisms may require up to 1 week, as they rely on phenotypic cha ... | 2015 | 24880875 |
| intramolecular aglycon delivery enables the synthesis of 6-deoxy-β-d-manno-heptosides as fragments of burkholderia pseudomallei and burkholderia mallei capsular polysaccharide. | burkholderia pseudomallei and burkholderia mallei are potential bioterrorism agents. they express the same capsular polysaccharide (cps), a homopolymer featuring an unusual [→3)-2-o-acetyl-6-deoxy-β-d-manno-heptopyranosyl-(1→] as the repeating unit. this cps is known to be one of the main targets of the adaptive immune response in humans and therefore represents a crucial subunit candidate for vaccine development. herein, the stereoselective synthesis of mono- and disaccharidic fragments of the ... | 2014 | 24786555 |
| evaluation of a latex agglutination assay for the identification of burkholderia pseudomallei and burkholderia mallei. | cases of melioidosis and glanders are rare in the united states, but the etiologic agents of each disease (burkholderia pseudomallei and burkholderia mallei, respectively) are classified as tier 1 select agents because of concerns about their potential use as bioterrorism agents. a rapid, highly sensitive, and portable assay for clinical laboratories and field use is required. our laboratory has further evaluated a latex agglutination assay for its ability to identify b. pseudomallei and b. mall ... | 2014 | 24710616 |
| population-sequencing as a biomarker of burkholderia mallei and burkholderia pseudomallei evolution through microbial forensic analysis. | large-scale genomics projects are identifying biomarkers to detect human disease. b. pseudomallei and b. mallei are two closely related select agents that cause melioidosis and glanders. accurate characterization of metagenomic samples is dependent on accurate measurements of genetic variation between isolates with resolution down to strain level. often single biomarker sensitivity is augmented by use of multiple or panels of biomarkers. in parallel with single biomarker validation, advances in ... | 2013 | 24455204 |
| burkholderia mallei and burkholderia pseudomallei cluster 1 type vi secretion system gene expression is negatively regulated by iron and zinc. | burkholderia mallei is a facultative intracellular pathogen that causes glanders in humans and animals. previous studies have demonstrated that the cluster 1 type vi secretion system (t6ss-1) expressed by this organism is essential for virulence in hamsters and is positively regulated by the virag two-component system. recently, we have shown that t6ss-1 gene expression is up-regulated following internalization of this pathogen into phagocytic cells and that this system promotes multinucleated g ... | 2013 | 24146925 |
| use of a safe, reproducible, and rapid aerosol delivery method to study infection by burkholderia pseudomallei and burkholderia mallei in mice. | burkholderia pseudomallei, the etiologic agent of melioidosis, is a saprophytic bacterium readily isolated from wet soils of countries bordering the equator. burkholderia mallei is a host-adapted clone of b. pseudomallei that does not persist outside of its equine reservoir and causes the zoonosis glanders, which is endemic in asia, africa, the middle east and south america. infection by these organisms typically occurs via percutaneous inoculation or inhalation of aerosols, and the most common ... | 2013 | 24098563 |
| revised structures for the predominant o-polysaccharides expressed by burkholderia pseudomallei and burkholderia mallei. | o-polysaccharides (ops) were isolated from purified burkholderia pseudomallei and burkholderia mallei lipopolysaccharides by mild-acid hydrolysis and gel-permeation chromatography. 1-d and 2-d (1)h and (13)c nmr spectroscopy experiments revealed that the ops antigens were unbranched heteropolymers with the following structures: collectively, our results demonstrate that the predominant ops antigens expressed by b. pseudomallei and b. mallei isolates are structurally more complex than previously ... | 2013 | 24056008 |
| animal models for francisella tularensis and burkholderia species: scientific and regulatory gaps toward approval of antibiotics under the fda animal rule. | the development and regulatory approval of medical countermeasures (mcms) for the treatment and prevention of bacterial threat agent infections will require the evaluation of products in animal models. to obtain regulatory approval, these models must accurately recapitulate aspects of human disease, including, but not necessarily limited to, route of exposure, time to disease onset, pathology, immune response, and mortality. this article focuses on the state of animal model development for 3 age ... | 2013 | 23628693 |
| trimethoprim/sulfamethoxazole (co-trimoxazole) prophylaxis is effective against acute murine inhalational melioidosis and glanders. | burkholderia pseudomallei is the causative agent of the disease melioidosis, which is prevalent in tropical countries and is intractable to a number of antibiotics. in this study, the antibiotic co-trimoxazole (trimethoprim/sulfamethoxazole) was assessed for the post-exposure prophylaxis of experimental infection in mice with b. pseudomallei and its close phylogenetic relative burkholderia mallei, the causative agent of glanders. co-trimoxazole was effective against an inhalational infection wit ... | 2013 | 23517714 |
| distinct human antibody response to the biological warfare agent burkholderia mallei. | the genetic similarity between burkholderia mallei (glanders) and burkholderia pseudomallei (melioidosis) had led to the general assumption that pathogenesis of each bacterium would be similar. in 2000, the first human case of glanders in north america since 1945 was reported in a microbiology laboratory worker. leveraging the availability of pre-exposure sera for this individual and employing the same well-characterized protein array platform that has been previously used to study a large cohor ... | 2012 | 23076276 |
| development of capsular polysaccharide-based glycoconjugates for immunization against melioidosis and glanders. | burkholderia pseudomallei and burkholderia mallei, the etiologic agents of melioidosis and glanders, respectively, cause severe disease in humans and animals and are considered potential agents of biological warfare and terrorism. diagnosis and treatment of infections caused by these pathogens can be challenging and, in the absence of chemotherapeutic intervention, acute disease is frequently fatal. at present, there are no human or veterinary vaccines available for immunization against these em ... | 2012 | 22912938 |
| the madagascar hissing cockroach as a novel surrogate host for burkholderia pseudomallei, b. mallei and b. thailandensis. | burkholderia pseudomallei and burkholderia mallei are gram-negative pathogens responsible for the diseases melioidosis and glanders, respectively. both species cause disease in humans and animals and have been designated as category b select agents by the centers for disease control and prevention (cdc). burkholderia thailandensis is a closely related bacterium that is generally considered avirulent for humans. while it can cause disease in rodents, the b. thailandensis 50% lethal dose (ld50) is ... | 2012 | 22892068 |
| microbial rhamnolipid production: a critical re-evaluation of published data and suggested future publication criteria. | high production cost and potential pathogenicity of pseudomonas aeruginosa, commonly used for rhamnolipid synthesis, have led to extensive research for safer producing strains and cost-effective production methods. this has resulted in numerous research publications claiming new non-pathogenic producing strains and novel production techniques many of which are unfortunately without proper characterisation of product and/or producing strain/s. genes responsible for rhamnolipid production have onl ... | 2017 | 28386631 |
| whole-genome sequences of burkholderia pseudomallei isolates exhibiting decreased meropenem susceptibility. | we report here paired isogenic burkholderia pseudomallei genomes obtained from three patients receiving intravenous meropenem for melioidosis treatment, with post-meropenem isolates developing decreased susceptibility. two genomes were finished, and four were drafted to improved high-quality standard. these genomes will be used to identify meropenem resistance mechanisms in b. pseudomallei. | 2017 | 28385830 |
| matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the identification of burkholderia pseudomallei from asia and australia and differentiation between burkholderia species. | matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) is increasingly used for rapid bacterial identification. studies of burkholderia pseudomallei identification have involved small isolate numbers drawn from a restricted geographic region. there is a need to expand the reference database and evaluate b. pseudomallei from a wider geographic distribution that more fully captures the extensive genetic diversity of this species. here, we describe the evaluatio ... | 2017 | 28384252 |
| biosynthesis of di-rhamnolipids and variations of congeners composition in genetically-engineered escherichia coli. | to engineer escherichia coli for the heterologous production of di-rhamnolipids, which are important biosurfactants but mainly produced by opportunistic pathogen pseudomonas aeruginosa. | 2017 | 28374071 |
| comparison of o-polysaccharide and hemolysin co-regulated protein as target antigens for serodiagnosis of melioidosis. | melioidosis is a severe disease caused by burkholderia pseudomallei. clinical manifestations are diverse and acute infections require immediate treatment with effective antibiotics. while culture is the current diagnostic standard, it is time-consuming and has low sensitivity. in endemic areas, inaccessibility to biosafety level 3 facilities and a lack of good serodiagnostic tools can impede diagnosis and disease surveillance. recent studies have suggested that o-polysaccharide (ops) and hemolys ... | 2017 | 28358816 |