| inhibition of growth of n. gonorrhoeae by bacterial interference. | observations made in our laboratory during the past 3 years showed that the growth of some neisseria gonorrhoeae cultures was inhibited in the presence of various bacterial contaminants of urogenital specimens. a total of 647 cultures confirmed as containing n. gonorrhoeae were examined in this study. the first part of the study was carried out on 520 n. gonorrhoeae cultures. of these 520 cultures, 110 (21.1%) showed contamination on purity plates (gc medium, antibiotic-free). when further subcu ... | 1976 | 824037 |
| bacteriolytic action of fluoride ions. | bacillus subtilis, neisseria subflava, and lyt coccus were found to undergo massive lysis after growth in media containing 0.01 to 10 mm naf. when cells of these organisms were transferred from late-exponential-phase cultures to 0.02 m sodium phosphate buffer plus 0.1 m kcl, they underwent spontaneous autolysis. cells grown in media with fluoride were more liable to autolysis, and walls isolated from them also showed enhanced autolytic sensitivity, even though added fluoride did not directly sti ... | 1977 | 907327 |
| [detection of meningococci in the sputum of bronchitis patients (author's transl)]. | within a period of only three months meningococci, sero b, c and also y, were detected among the patients of a hospital. the meningococci were isolated as sole pathogen, or in association with other bronchopathogens in five cases of purulent respiratory tract infection, most of whom were suffering from acute or chronic bronchitis. the pathogens were initially identified falsely as "facultative" neisseria subflava. it is stressed that meningococci must be considered as possible pathogens in such ... | 1977 | 914365 |
| neisseria subflava infections--bacteriological aspects of two cases. | | 1975 | 1224640 |
| production and characterisation of mouse monoclonal antibodies reactive with the lipopolysaccharide core of pseudomonas aeruginosa. | monoclonal antibodies (mabs) to the core antigen region of lipopolysaccharide (lps) of pseudomonas aeruginosa were produced from mice immunised with whole cells of heat-killed rough mutants of pseudomonas aeruginosa expressing partial or complete core lps. mabs were screened in an enzyme-linked immunosorbent assay (elisa) against three different antigen cocktails: s-form lps from three p. aeruginosa strains, r-form lps from six p. aeruginosa strains and, as a negative control, r-form lps from sa ... | 1992 | 1588587 |
| frequent isolation of capnophilic bacteria in aspirate from bartholin's gland abscesses and cysts. | aspirate samples were obtained from 73 patients undergoing surgery for a bartholin's gland abscess or cyst. capnophilic bacteria were isolated from 22% of cases, in contrast to previous reports of sporadic isolation. the three streptococcus pneumoniae isolates were serotype 3 or 4. the eight haemophilus influenzae isolates could not be serotyped; six of them were biotype ii but the electrophoretic band patterns of their outer membrane proteins were heterogeneous, indicating that non-serotypable ... | 1990 | 2108028 |
| the effect of pasteurella haemolytica and the leukotoxin of pasteurella haemolytica on bovine lung explants. | bovine lung explants were used in a study designed to compare the pathogenic effects of pasteurella haemolytica type 1, a nonpathogenic organism neisseria subflava, or the crude leukotoxin of p. haemolytica on alveolar macrophages and lung parenchymal cells. concentrated, purified peripheral blood neutrophil suspensions were added with the bacteria to some explants. duplicate pairs of cultures from each treatment group were fixed at regular intervals up to 24 hours after seeding and morphologica ... | 1990 | 2306666 |
| unusual pathogens in narcotic-associated endocarditis. | we report the cases of three adults with a history of intravenous drug abuse who developed endocarditis caused by corynebacterium xerosis, neisseria subflava, and neisseria flavescens, respectively. no cases of endocarditis caused by c. xerosis or n. flavescens and only one case caused by n. subflava have previously been reported in association with narcotic addiction. the prominent clinical features in all patients included poor response to antibiotic therapy, persistent fever, and major emboli ... | 1990 | 2359905 |
| erythrocyte gangliosides act as receptors for neisseria subflava: identification of the sia-1 adhesin. | neisseria gonorrhoeae was recently shown to bind to a subset of lactose-containing glycolipids (n. strömberg, c. deal, g. nyberg, s. normark, m. so, and k.-a. karlsson, proc. natl. acad. sci. usa 85:4902-4906, 1988). a number of commensal neisseria strains were also shown to be lactose binders. in addition, neisseria subflava bound to immobilized gangliosides, such as hematoside and sialosyl paragloboside, carrying the neuac alpha 2-3gal beta 1-4glc sequence. to a lesser extent, n. gonorrhoeae a ... | 1990 | 2370108 |
| pharyngitis-associated m-12 group a streptococcus satellite strain: association with neisseria subflava. | | 1989 | 2594456 |
| [endocarditis caused by neisseria subflava]. | | 1989 | 2623270 |
| analysis of damage to human ciliated nasopharyngeal epithelium by neisseria meningitidis. | we used an in vitro model of human nasopharyngeal tissue in organ culture to evaluate the effects of neisseria meningitidis on human cilia and ciliary function. encapsulated, viable meningococci damaged ciliated epithelium of nasopharyngeal organ cultures, whereas neisseria subflava, a commensal species, did not. meningococcus-induced ciliary damage was due to loss of ciliated cells to which meningococci were not attached. damage was seen with piliated and nonpiliated meningococci and did not ap ... | 1986 | 2867973 |
| nitrite accumulation during anaerobic nitrate reduction by binary suspensions of bacteria isolated from the achlorhydric stomach. | binary suspensions of bacteria isolated from the gastric juice of achlorhydric patients were used to determine conditions which favour nitrite accumulation during nitrate reduction. suspensions of veillonella parvula and haemophilus parainfluenzae accumulated nitrite during nitrate reduction in the absence of nitrite-reducing neisseria subflava or streptococcus sanguis. the maximum concentration of nitrite that transiently accumulated decreased predictably as the ratio of nitrite-removing bacter ... | 1987 | 3117970 |
| evaluation of a ten-minute chromogenic substrate test for identification of pathogenic neisseria species and branhamella catarrhalis. | a ten-minute chromogenic substrate test was evaluated for its ability to rapidly identify pathogenic neisseria spp. and branhamella catarrhalis. identifications obtained with this system were compared to those obtained using conventional procedures. the test correctly identified 98.9% of 90 neisseria gonorrhoeae, 98.3% of 60 neisseria meningitidis, 96.2% of 26 neisseria lactamica, and 100% of 36 branhamella catarrhalis strains. eight neisseria subflava strains that grew on modified thayer-martin ... | 1988 | 3132371 |
| [neonatal meningitis caused by neisseria subflava]. | | 1988 | 3149878 |
| enzymatic modification of aminoglycoside antibiotics by branhamella catarrhalis carrying an r factor. | fifteen out of 89 clinical strains of branhamella catarrhalis isolated from patients at the university hospital of zaragoza were resistant to aminoglycosides and other antimicrobials. in two strains, b. catarrhalis 220 and b. catarrhalis 115, the resistance to aminoglycosides was associated with synthesis of aminoglycoside-modifying enzymes, namely 3"-o-phosphotransferase [aph(3")] and 3'-o-phosphotransferase [aph(3')]. b. catarrhalis 115 was resistant to ampicillin, streptomycin, kanamycin, neo ... | 1987 | 3304917 |
| asplenia in a patient with neisseria subflava endocarditis. | | 1987 | 3578352 |
| efficacy of imipenem/cilastatin in endocarditis. | imipenem, a potent new beta-lactam antibiotic, which is bactericidal against most pathogenic bacteria, and cilastatin, a dehydropeptidase inhibitor combined with imipenem to prevent the metabolism of imipenem in the kidney, were evaluated in the treatment of bacterial endocarditis. seventeen patients, including 14 who used intravenous drugs, were treated with imipenem/cilastatin in a dose of 500 mg each infused over 30 minutes every six hours. the mean duration of treatment was 29 days with a ra ... | 1985 | 3859210 |
| evaluation of a rapid identification method for neisseria spp. | a comparison was made of carbohydrate degradation reactions of neisseria gonorrhoeae, neisseria meningitidis, neisseria lactamica, neisseria mucosa, neisseria sicca, neisseria subflava, and branhamella catarrhalis in a rapid (0.5- to 1-h) identification micromethod (rim-n kit; austin biological laboratories, inc., austin, tex.) and in a serum-free agar slanted medium (72 h). reactions after 1 h in the rim-n system agreed completely with those after 72 h in the conventional system. | 1985 | 3891778 |
| superoxide dismutase and oxygen toxicity defenses in the genus neisseria. | among aerotolerant cells, neisseria gonorrhoeae is very unusual because despite its obligately aerobic lifestyle and frequent isolation from purulent exudates containing polymorphonuclear leukocytes vigorously evolving o2- and h2o2, it contains no superoxide dismutase (sod). strains (14) of n. gonorrhoeae were compared with each other and with strains of neisseria meningitidis, neisseria mucosa, and neisseria subflava under identical growth conditions for their contents of the oxy-protective enz ... | 1986 | 3943903 |
| [acute neisseria subflava endocarditis]. | a 50-year-old woman who developed acute endocarditis on a mitral starr valve due to neisseria subflava is reported. this microorganism, which belongs to the normal flora of the upper respiratory tract, is a very rare cause of endocarditis since only 9 cases have been described. the patient developed various complications including right hemiplegia. cure was obtained by medical therapy alone due to the high susceptibility of the bacteria to a third generation cephalosporin (moxalactam). this is t ... | 1986 | 3952472 |
| neisseria subflava bacteremia and meningitis in a child: report of a case and review of the literature. | | 1985 | 4000991 |
| purpura and septicemia due to neisseria subflava. | | 1968 | 5646715 |
| neisseria subflava endocarditis. case report and review of the literature. | neisseria subflava is a rare cause of bacterial endocarditis. only seven cases have been identified in the world literature if strict criteria as to organism classification and of endocarditis are applied to individual case reports. the first reported case in an intravenous drug user is described. in addition, findings on serial two-dimensional and m-mode echocardiography performed during and after the treatment period are presented. this patient's recovery with anti-microbial therapy alone desp ... | 1984 | 6369982 |
| the significance of nongonococcal, nonmeningococcal neisseria isolates from blood cultures. | nongonococcal, nonmeningococcal neisseriae are part of the normal respiratory flora and infrequently cause disease. these organisms include neisseria lactamica, neisseria mucosa, neisseria sicca, neisseria flavescens, neisseria subflava , neisseria perflava , neisseria flava , and branhamella catarrhalis (previously classified as neisseria catarrhalis). blood cultures positive for these bacteria have been associated with serious infections, including endocarditis, septicemia, and meningitis. in ... | 1984 | 6374834 |
| conjugation of plasmids of neisseria gonorrhoeae to other neisseria species: potential reservoirs for the beta-lactamase plasmid. | the discovery that penicillinase production in neisseria gonorrhoeae was plasmid mediated and the spread of the beta-lactamase encoding plasmids in gonococcal isolates since 1976, raise the possibility that a nonpathogenic indigenous bacterium could serve as a reservoir for these plasmids. we initiated studies to define the ability of commensal neisseria species and branhamella catarrhalis strains, as well as strains of the pathogen neisseria meningitidis, to serve as recipients in conjugation w ... | 1984 | 6434640 |
| diagnostic value of interactions between members of the family neisseriaceae and lectins. | the lectin slide agglutination test for neisseria gonorrhoeae has been modified and improved. results show that wheat germ agglutinin and soybean lectin agglutinate 100% (193 of 193 tested) of clinical isolates of n. gonorrhoeae. lectin-reactive meningococci can be readily identified by the hydrolysis of gamma-glutamyl-beta-naphthylamide. branhamella catarrhalis, neisseria lactamica, neisseria sicca, neisseria subflava, neisseria perflava, and meningococcal serogroups a, b, c, x, y, and z do not ... | 1984 | 6546936 |
| neisseria subflava endophthalmitis. | | 1982 | 6982003 |
| neisseria subflava endocarditis. | | 1981 | 7212904 |
| phylogenetic relationships between some members of the genera neisseria, acinetobacter, moraxella, and kingella based on partial 16s ribosomal dna sequence analysis. | we obtained 16s ribosomal dna (rdna) sequence data for strains belonging to 11 species of proteobacteria, including the type strains of kingella kingae, neisseria lactamica, neisseria meningitidis, moraxella lacunata subsp. lacunata, [neisseria] ovis, moraxella catarrhalis, moraxella osloensis, [moraxella] phenylpyruvica, and acinetobacter lwoffii, as well as strains of neisseria subflava and acinetobacter calcoaceticus. the data in a distance matrix constructed by comparing the sequences suppor ... | 1994 | 7520730 |
| neisseria subflava causing septic arthritis of the ankle in a child. | | 1993 | 8370934 |
| asymptomatic neisseria subflava biovar perflava bacteriuria in a child with obstructive uropathy. | a case of asymptomatic urinary tract infection with neisseria subflava biovar perflava in a 10-year-old male patient with congenital structural abnormalities of the urinary bladder is presented. the organism was recovered from three catheter urine specimens collected over a seven-month period. a brief review of the role of saprophytic neisseria species in infectious processes is presented and the likely source of this unusual urinary tract isolate is discussed. | 1993 | 8404915 |
| nonfebrile mitral valve endocarditis due to neisseria subflava. | native valve endocarditis normally presents with fever and only later in its course demonstrates dysfunction of the affected valve. we describe a case of endocarditis due to neisseria subflava, a gram-negative diplococcal saprophyte of the oral cavity, which was unsuspected clinically and found unexpectedly during a mitral valve operation performed for symptomatic prolapse with regurgitation. | 1996 | 8549200 |
| neisseria subflava bacteremia in a neutropenic patient. | | 1996 | 8694678 |
| [bacteremia caused by neisseria subflava and neutrocytic ascites in a female patient with hepatic cirrhosis]. | | 1996 | 8744381 |
| effect of oxygen, inoculum composition and flow rate on development of mixed-culture oral biofilms. | the effect of aeration on the development of a defined biofilm consortium of oral bacteria was investigated in a two-stage chemostat system. an inoculum comprising 10 species, including both facultatively anaerobic and obligately anaerobic bacteria, and species associated with oral health and disease, was inoculated into an anaerobic first-stage chemostat vessel. the effluent from this chemostat was linked to an aerated [200 ml co2/air (5:95, v/v) min-1] second-stage vessel, in which removable h ... | 1996 | 8868437 |
| development of a steady-state oral microbial biofilm community using the constant-depth film fermenter. | the complexity of biofilm communities like dental plaque suggests that laboratory model biofilm growth systems may help to understand their structure and function. this study describes the use of a constant-depth film fermenter (cdff) to investigate biofilm formation by a nine-membered community of oral bacteria. the community was grown to steady state in a chemostat incubated anaerobically. the chemostat output was fed into the cdff incubated aerobically. viable counts for each species from the ... | 1996 | 8868438 |
| the prevalence of gentamicin 2'-n-acetyltransferase in the proteeae and its role in the o-acetylation of peptidoglycan. | the prevalence of aac(2')-ia, a gene coding for gentamicin 2'-n-acetyltransferase in providencia stuartii, among species of the proteeae was investigated to determine if it is a common resistance factor and whether the correlation observed in p. stuartii between its expression and the levels of peptidoglycan o-acetylation represents a general feature of bacteria producing this form of modified peptidoglycan. an evaluation of the mics of gentamicin for each of the species of the proteeae did not ... | 1996 | 8961557 |
| stoichiometry of oxygen consumption and sugar, organic acid and amino acid utilization in salivary sediment and pure cultures of oral bacteria. | in each of 23 numerically or metabolically significant oral micro-organisms, and in each of the salivary sediments of 10 humans, oxygen uptake was determined quantitatively with various sugar and organic and amino acid substrates. with relatively few exceptions, the salivary sediments rapidly consumed oxygen with the array of substrates (23) tested. on the other hand, the individual pure cultures oxidized fewer substrates and did so selectively from this menu. the observation that the gram-posit ... | 1996 | 9031704 |
| [infectious endocarditis by neisseria subflava in two hiv drug users]. | | 1997 | 9235111 |
| analysis of ph-driven disruption of oral microbial communities in vitro. | previously, a mixed culture chemostat system was used to demonstrate that the ph generated from carbohydrate metabolism, rather than carbohydrate availability per se, was responsible for the shifts observed in the oral microflora which are associated with high carbohydrate diets and the development of dental caries. the aim of this study was to determine more accurately the microbially generated ph at which such shifts occurred. nine oral bacteria were grown in three independent chemostats, and ... | 1998 | 9745120 |
| evaluation of amplicor neisseria gonorrhoeae pcr using cppb nested pcr and 16s rrna pcr. | certain strains of neisseria subflava and neisseria cinerea are known to produce false-positive results with the amplicor neisseria gonorrhoeae pcr (roche diagnostic systems, branchburg, n.j.). the analytical sensitivity and analytical specificity of three pcr tests were assessed with 3 geographically diverse n. gonorrhoeae strains and 30 non-n. gonorrhoeae neisseria spp. the sensitivities of the in-house nested cppb gene and the 16s rrna pcr methods were greater than that of the amplicor n. gon ... | 1999 | 9889224 |
| binding of n-trifluoroacetyl-derivatized natural glycosphingolipids by uropathogenic escherichia coli and neisseria subflava. | several neutral glycosphingolipids were hydrogenated and subjected to trifluoroacetylation in trifluoroacetic acid/trifluoroacetic acid anhydride under conditions leading to complete exchange of the n-acetyl groups of galnac for n-trifluoroacetyl. the derivatized glycosphingolipids were analyzed for binding by p-fimbriated uropathogenic escherichia coli, recognizing the globo series of glycolipids (carrying galalpha1-4gal). using e. coli it was shown that a galnco-cf3 next to the minimum binding ... | 1998 | 10211707 |
| neisseria subflava biovar perflava peritonitis in a continuous cyclic peritoneal dialysis patient. | | 1999 | 10383043 |
| meningitis due to neisseria subflava: case report and review. | | 2000 | 10722463 |
| the formation of mixed culture biofilms of oral species along a gradient of shear stress. | a chemostat mixed culture system was used to produce two distinct ecological states, state-1 (caries-like microcosm) and state-2 (periodontal-like microcosm). eleven bacterial species (streptococcus gordonii, strep. mitis i, strep. mutans, strep. oralis, actinomyces naeslundii, lactobacillus casei, neisseria subflava, fusobacterium nucleatum, porphyromonas gingivalis, prevotella nigrescens, veillonella dispar) were used to inoculate the planktonic system. a flow cell, designed to produce converg ... | 2000 | 11054158 |
| degradation of heme in gram-negative bacteria: the product of the hemo gene of neisseriae is a heme oxygenase. | a full-length heme oxygenase gene from the gram-negative pathogen neisseria meningitidis was cloned and expressed in escherichia coli. expression of the enzyme yielded soluble catalytically active protein and caused accumulation of biliverdin within the e. coli cells. the purified hemo forms a 1:1 complex with heme and has a heme protein spectrum similar to that previously reported for the purified heme oxygenase (hmuo) from the gram-positive pathogen corynebacterium diphtheriae and for eukaryot ... | 2000 | 11073924 |
| structural and immunochemical characterization of the lipooligosaccharides expressed by neisseria subflava 44. | neisserial lipooligosaccharides (loss) are a family of complex cell surface glycolipids. we used mass spectrometry techniques (electrospray ionization, collision-induced dissociation, and multiple step), combined with fluorophore-assisted carbohydrate electrophoresis monosaccharide composition analysis, to determine the structure of the two low-molecular-mass los molecules (losi and losii) expressed by neisseria subflava 44. we determined that losi contains one glucose on both the alpha and beta ... | 2001 | 11208793 |
| haemophilus segnis polymicrobial and monomicrobial bacteraemia identified by 16s ribosomal rna gene sequencing. | this paper reports a case of haemophilus segnis polymicrobial bacteraemia and a case of h. segnis monomicrobial bacteraemia identified by 16s ribosomal rna gene sequencing. in the first case, a gram-negative aerobic coccobacillus was isolated with streptococcus intermedius and s. sanguis from the blood culture of a 32-year-old intravenous drug addict with left thoracic empyema. in the second case, a gram-negative aerobic coccobacillus was isolated from the blood culture of an 82-year-old woman w ... | 2002 | 12171293 |
| biofilm dispersal of neisseria subflava and other phylogenetically diverse oral bacteria. | polystyrene petri dishes containing liquid medium were inoculated with single-cell suspensions of a fresh clinical isolate of neisseria subflava and were incubated under conditions of low vibration. n. subflava colonies grew firmly attached to the surface of the dish, while the broth remained clear. growing colonies released cells into the medium, resulting in the appearance of 10(2) to 10(4) small satellite colonies attached to the surface of the dish in an area adjacent to each mature colony a ... | 2002 | 12324342 |
| neisseria subflava as a cause of meningitis and septicemia in children. report of five cases. | in five cases of meningitis or septicemia in children, the causative organism was neisseria subflava. the organism is similar to n meningitidis. only one previous case of meningitis caused by n subflava in a child has been found in the literature, but this organism may be more commonly pathogenic than has been thought. the clinical findings and course are not unlike those of septicemia and meningitis due to n meningitidis. | 1966 | 12608164 |
| effects of a chlorhexidine gluconate-containing mouthwash on the vitality and antimicrobial susceptibility of in vitro oral bacterial ecosystems. | oral bacterial microcosms, established using saliva inocula from three individuals, were maintained under a feast-famine regime within constant-depth film fermenters. steady-state communities were exposed four times daily, postfeeding, to a chlorhexidine (chx) gluconate-containing mouthwash (chxm) diluted to 0.06% (wt/vol) antimicrobial content. the microcosms were characterized by heterotrophic plate counts and pcr-denaturing gradient gel electrophoresis (dgge). chxm caused significant decrease ... | 2003 | 12902270 |
| post traumatic polymicrobial endophthalmitis, including neisseria subflava. | to report the second known case of post-traumatic endophthalmitis caused by neisseria subflava. | 2003 | 12967818 |
| effects of triclosan-containing rinse on the dynamics and antimicrobial susceptibility of in vitro plaque ecosystems. | dental plaque microcosms were established under a feast-famine regimen within constant-depth film fermentors and exposed four times daily postfeeding to a triclosan (tr)-containing rinse (dentifrice) (trd). this was diluted so that the antimicrobial content was 0.6 mg/ml. microcosms were characterized by heterotrophic plate counts and pcr-denaturing gradient gel electrophoresis (dgge) with primers specific for the v2-v3 region of the eubacterial 16s rrna gene (rdna). dominant isolates and pcr am ... | 2003 | 14576113 |
| selection for high-level resistance by chronic triclosan exposure is not universal. | to investigate the effect of triclosan exposure on the antimicrobial susceptibilities of numerically important dental bacteria. | 2004 | 15117935 |
| [bacteremia due to neisseria subflava biovar flava]. | | 2004 | 15274890 |
| functional diversity of three different dsba proteins from neisseria meningitidis. | the genome of neisseria meningitidis serogroup b strain mc58 contains three genes - nmb0278, nmb0294 and nmb0407 - encoding putative homologues of dsba, a periplasmic thiol disulphide oxidoreductase protein-folding catalyst of the dsb protein family. dsba assists the folding of periplasmic and membrane proteins in diverse organisms. while all three cloned genes complemented the dtt sensitivity of dsba-null escherichia coli, they showed different activities in folding specific target proteins in ... | 2004 | 15347757 |
| vasculitic purpura with antineutrophil cytoplasmic antibody-positive acute renal failure in a patient with streptococcus bovis case and neisseria subflava bacteremia and subacute endocarditis. | subacute bacterial endocarditis is frequently associated with extracardiac manifestations and renal failure. clinical variety of endocarditis manifestation is wide and has the potential to mimic vasculitis. whereas streptococcus bovis is often isolated and associated with colonic tumors, neisseriaceae are rarely found. an association of subacute bacterial endocarditis and antineutrophil cytoplasmic antibodies has been described. we report on a 62-year-old man who was admitted to our hospital wit ... | 2004 | 15356972 |
| [neisseria subflava subsp. perfiava bacteremia: a case study and literature review]. | | 2004 | 15679240 |
| analysis of mutations within multiple genes associated with resistance in a clinical isolate of neisseria gonorrhoeae with reduced ceftriaxone susceptibility that shows a multidrug-resistant phenotype. | a neisseria gonorrhoeae strain with a reduced susceptibility to ceftriaxone (minimum inhibitory concentration (mic) = 0.5 microg/ml) was isolated among 398 clinical isolates obtained from 2000-2001 in fukuoka city, japan. the n. gonorrhoeae strain was negative for penicillinase production but it showed multidrug resistance against penicillin (mic = 8 microg/ml), tetracycline (mic = 4 microg/ml), azithromycin (mic = 0.5 microg/ml) and ciprofloxacin (mic = 16 microg/ml). the molecular mechanisms o ... | 2006 | 16318912 |
| variations in gene organization and dna uptake signal sequence in the folp region between commensal and pathogenic neisseria species. | horizontal gene transfer is an important source of genetic variation among neisseria species and has contributed to the spread of resistance to penicillin and sulfonamide drugs in the pathogen neisseria meningitidis. sulfonamide resistance in neisseria meningitidis is mediated by altered chromosomal folp genes. at least some folp alleles conferring resistance have been horizontally acquired from other species, presumably from commensal neisseriae. in this work, the dna sequence surrounding folp ... | 2006 | 16503987 |
| multiparameter assessments to determine the effects of sugars and antimicrobials on a polymicrobial oral biofilm. | clinical studies indicate relationships between dental plaque, a naturally formed biofilm, and oral diseases. the crucial role of nonmicrobial biofilm constituents in maintaining biofilm structure and biofilm-specific attributes, such as resistance to shear and viscoelasticity, is increasingly recognized. concurrent analyses of the diverse nonmicrobial biofilm components for multiparameter assessments formed the focus of this investigation. comparable numbers of actinomyces viscosus, streptococc ... | 2006 | 17021225 |
| epidural abscess, discitis and vertebral osteomyelitis caused by neiserria subflava. | neisseria subflava is generally regarded as commensal flora of the oropharyngeal tract and usually non-pathogenic. there are, however, reports of invasive disease such as bacteremia, endocarditis, sepsis, meningitis, septic arthritis and discitis caused by this organism. this paper presents the first reported case of an epidural abscess caused by neiserria subflava. | 2007 | 17727199 |
| antimicrobial resistance in clinical isolates of neisseria subflava from the oral cavities of a japanese population. | a recent study indicated that neisseria subflava, one of the commensal neisseria species, may play an important role in the emergence of neisseria gonorrhoeae strains with chromosomally mediated resistance to penicillin or cephalosporin by the horizontal genetic exchange of pena genes encoding the target site for penicillin or cephalosporin. the present investigation examined the antimicrobial susceptibility of 45 isolates of n. subflava from the oral cavities of japanese men and women to variou ... | 2007 | 17982718 |
| biochemical and genomic analysis of the denitrification pathway within the genus neisseria. | since neisseria gonorrhoeae and neisseria meningitidis are obligate human pathogens, a comparison with commensal species of the same genus could reveal differences important in pathogenesis. the recent completion of commensal neisseria genome draft assemblies allowed us to perform a comparison of the genes involved in the catalysis, assembly and regulation of the denitrification pathway, which has been implicated in the virulence of several bacteria. all species contained a highly conserved nitr ... | 2009 | 19762442 |
| neisseria gonorrhoeae : detection and typing by probe hybridization, lcr, and pcr. | neisseria gonorrhoeae, first described by neisser in 1879, is a gram-negative, nonmotile, nonspore-forming diplococcus, belonging to the family neisseriaceae. it is the etiologic agent of gonorrhea. the other pathogenic species is neisseria meningitidis, to which n. gonorrhoeae is genetically closely related. although n. meningitidis is not usually considered to be a sexually transmitted disease, it may infect the mucous membranes of the anogenital area of homosexual men (1). the other members o ... | 1999 | 21390726 |
| a case of meningitis due to neisseria subflava after ventriculostomy. | surgical site infection (ssi) is an emerging problem in all fields of surgery, as well as in neurosurgery, with numerous strategies against ssi initiated in many countries. in this report, we describe a case of ssi caused by neisseria subflava a month after ventriculostomy placement for acute hydrocephalus. a 53-year old man was referred to our hospital, and was diagnosed with a thalamic hemorrhage with ventricular rupture. bilateral ventriculostomies were performed on the day of presentation. a ... | 2011 | 21894453 |
| enhanced gonococcal antimicrobial surveillance in the era of ceftriaxone resistance: a real-time pcr assay for direct detection of the neisseria gonorrhoeae h041 strain. | objectives: recent emergence of the extensively drug-resistant neisseria gonorrhoeae h041 strain in japan raises concerns that gonorrhoea may soon become untreatable and emphasizes the need for enhanced surveillance. in this study we developed a real-time pcr assay for direct detection of the h041 strain. methods: two real-time pcr assays for detection of the pena gene of the h041 strain, h041-pcr1 and h041-pcr2, were developed and evaluated in parallel. assay performance was assessed using a pa ... | 2011 | 22207596 |
| oral microbiota shift after 12-week supplementation with lactobacillus reuteri dsm 17938 and pta 5289; a randomized control trial. | lactobacillus spp. potentially contribute to health by modulating bacterial biofilm formation, but their effects on the overall oral microbiota remain unclear. | 2015 | 25946126 |
| effects of high hydrostatic pressure on bacterial growth on human ossicles explanted from cholesteatoma patients. | high hydrostatic pressure (hhp) treatment can eliminate cholesteatoma cells from explanted human ossicles prior to re-insertion. we analyzed the effects of hhp treatment on the microbial flora on ossicles and on the planktonic and biofilm states of selected isolates. | 2012 | 22291908 |
| diffusible signal factor (dsf) quorum sensing signal and structurally related molecules enhance the antimicrobial efficacy of antibiotics against some bacterial pathogens. | extensive use of antibiotics has fostered the emergence of superbugs that are resistant to multidrugs, which becomes a great healthcare and public concern. previous studies showed that quorum sensing signal dsf (diffusible signal factor) not only modulates bacterial antibiotic resistance through intraspecies signaling, but also affects bacterial antibiotic tolerance through interspecies communication. these findings motivate us to exploit the possibility of using dsf and its structurally related ... | 2014 | 24575808 |
| analytical evaluation of genexpert ct/ng, the first genetic point-of-care assay for simultaneous detection of neisseria gonorrhoeae and chlamydia trachomatis. | genexpert ct/ng was evaluated with 372 characterized bacterial strains. sensitivity of 10 genome copies/reaction was obtained for both agents. four neisseria mucosa and two neisseria subflava isolates were positive for one of two gonococcal targets; however, the assay flagged all as negative. the assay was analytically highly sensitive and specific. | 2013 | 23554203 |
| a comparative structure/function analysis of two type iv pilin dna receptors defines a novel mode of dna binding. | dna transformation is a widespread process allowing bacteria to capture free dna by using filamentous nano-machines composed of type iv pilins. these proteins can act as dna receptors as demonstrated by the finding that neisseria meningitidis comp minor pilin has intrinsic dna-binding ability. comp binds dna better when it contains the dna-uptake sequence (dus) motif abundant in this species genome, playing a role in its trademark ability to selectively take up its own dna. here, we report high- ... | 2016 | 27161979 |
| genome sequence analyses show that neisseria oralis is the same species as 'neisseria mucosa var. heidelbergensis'. | phylogenies generated from whole genome sequence (wgs) data provide definitive means of bacterial isolate characterization for typing and taxonomy. the species status of strains recently defined with conventional taxonomic approaches as representing neisseria oralis was examined by the analysis of sequences derived from wgs data, specifically: (i) 53 neisseria ribosomal protein subunit (rps) genes (ribosomal multi-locus sequence typing, rmlst); and (ii) 246 neisseria core genes (core genome mlst ... | 2013 | 24097834 |
| identification of an antibacterial protein by functional screening of a human oral metagenomic library. | screening of a bacterial artificial chromosome (bac) library containing metagenomic dna from human plaque and saliva allowed the isolation of four clones producing antimicrobial activity. three of these were pigmented and encoded homologues of glutamyl-trna reductase (glutr), an enzyme involved in the c5 pathway leading to tetrapyrole synthesis, and one clone had antibacterial activity with no pigmentation. the latter contained a bac with an insert of 15.6 kb. initial attempts to localize the ge ... | 2015 | 26347298 |
| chitosan-thioglycolic acid as a versatile antimicrobial agent. | as functionalized chitosans hold great potential for the development of effective and broad-spectrum antibiotics, representative chitosan derivatives were tested for antimicrobial activity in neutral media: trimethyl chitosan (tmc), carboxy-methyl chitosan (cmc), and chitosan-thioglycolic acid (tga; medium molecular weight: mmw-tga; low molecular weight: lmw-tga). colony forming assays indicated that lmw-tga displayed superior antimicrobial activity over the other derivatives tested: a 30 min in ... | 2013 | 23470196 |
| development of a novel engineered antibody targeting neisseria species. | a neissaria bacterial pilus sugar, bacillosamine, was synthesized and, for the first time, used as a probe to screen a single-chain variable fragment (scfv). | 2017 | 27888365 |
| changes in microbiota during experimental human rhinovirus infection. | human rhinovirus (hrv) is responsible for the majority of common colds and is frequently accompanied by secondary bacterial infections through poorly understood mechanisms. we investigated the effects of experimental human hrv serotype 16 infection on the upper respiratory tract microbiota. | 2015 | 26271750 |
| the first case report of acute cholangitis and bacteremia due to neisseria subflava. | we herein report a case of acute cholangitis and bacteremia caused by a commensal neisseria species, neisseria subflava, in an 82-year-old man with cholangiocarcinoma. emergency endoscopic nasobiliary drainage and cefoperazone/sulbactam therapy were effective. gram negative coccobacilli were isolated from both blood and bile cultures on 5% sheep blood agar. the isolate was identified as n.subflava biovar perflava by mass spectrometry, a sequence analysis of the 16s rrna, and biochemical testing. ... | 2017 | 28090057 |