| chloramphenicol-induced stabilization of cat messenger rna in bacillus subtilis. | the expression of the chloramphenicol-inducible chloramphenicol-acetyltransferase gene (cat), encoded on staphylococcus aureus plasmid pub112, is regulated via a translational attenuation mechanism. ribosomes, which are arrested by chloramphenicol during synthesis of a short leader peptide, activate catmrna translation by opening a 5'-located stem-loop structure, thus setting free the cat ribosome-binding site. we have determined the 5' and 3' ends of catmrna and analysed its stability in bacill ... | 1991 | 1809841 |
| chloramphenicol-induced translational activation of cat messenger rna in vitro. | the expression of the chloramphenicol-inducible chloramphenicol acetyltransferase gene (cat) of the staphylococcal plasmid pub112 is regulated at the post-transcriptional level. previous in vivo analyses suggested that the antibiotic stalls ribosomes that are translating a regulatory leader peptide, and that a stalled ribosome activates the ribosome binding site of the acetyltransferase encoding sequence by opening an attenuating leader mrna hairpin structure. to test this model, we used a bacil ... | 1990 | 2109801 |
| positioning ribosomes on leader mrna for translational activation of the message of an inducible staphylococcus aureus cat gene. | the expression of the chloramphenicol (cm) - inducible cm acetyltransferase gene (cat) of the staphylococcal plasmid pub112 is regulated at the translational level. the leader mrna preceding the cat coding sequence can form a stable hairpin structure, in which the cat shine-dalgarno sequence is masked. previous work showed that translation of a short leader peptide terminating within the stem of the inhibitory secondary structure is required for basal cm acetyltransferase (cat) synthesis and its ... | 1988 | 2465483 |
| dependence of expression of an inducible staphylococcus aureus cat gene on the translation of its leader sequence. | the gene for chloramphenicol (cm) acetyltransferase (cat) carried by the staphylococcal plasmid pub112, whose expression can be stimulated by cm, is preceded by a regulatory region containing two control elements. one of these consists of a shine-dalgarno (sd) sequence followed by an open reading frame coding for a leader peptide of nine amino acids. previous work has shown that the sd sequence is essential for inducibility of cm resistance by the antibiotic (brückner and matzura 1985). here we ... | 1987 | 3112518 |
| regulation of the inducible chloramphenicol acetyltransferase gene of the staphylococcus aureus plasmid pub112. | analyses of deletion mutants of the gene for chloramphenicol (cm) acetyltransferase (cat) carried by the staphylococcal plasmid pub112 revealed a regulatory region, which is indispensable for cm-inducible cat gene expression, located 70 bp in front of the cat-coding sequence. this region consists of a possible ribosome binding site followed by an open reading frame coding for a peptide of nine amino acids and overlaps partially with an inverted repeat capable of forming a stem-loop structure. de ... | 1985 | 3865770 |
| expression of a chloramphenicol-resistance determinant carried on hybrid plasmids in gram-positive and gram-negative bacteria. | to analyse the control of chloramphenicol (cm) resistance conferred by the staphylococcus aureus plasmid pub112, a detailed restriction map of this plasmid has been constructed, and the position and orientation of the cat gene have been determined. an mboi restriction fragment carrying the entire cat gene of pub112 was then cloned in another s. aureus plasmid, the kanamycin (km) resistance vector pub110. depending on the orientation of the incorporated cat fragment, the level of cm resistance va ... | 1984 | 6442250 |