| diaphorase activity located in inclusions induced by soil-borne wheat mosaic virus. | | 1976 | 7878 |
| the relationship between soil-borne wheat mosaic virus and tobacco mosaic virus. | | 1976 | 936469 |
| electron microscopy of soil-borne wheat mosaic virus in host cells. | | 1970 | 4099066 |
| purification and properties of soil-borne wheat mosaic virus. | | 1971 | 4107550 |
| comparative electron microscopy of cytoplasmic inclusions induced by 9 isolates of soil-borne wheat mosaic virus. | | 1974 | 4131630 |
| electron microscopy of inclusion development in rye leaf cells infected with soil-borne wheat mosaic virus. | | 1974 | 4131631 |
| correlation of polymyxa graminis with transmission of soil-borne wheat mosaic virus. | | 1966 | 5949390 |
| primary structure and sequence analysis of rna2 of a mechanically transmitted barley mild mosaic virus isolate: an evolutionary relationship between bymo- and furoviruses. | the rna2 nucleotide sequence of a mechanically transmitted isolate of barley mild mosaic virus (bammv) has been determined. a combination of northern blot and sequence analysis indicates that this rna2 lacks approximately 1000 nucleotides of its c-terminal protein (p2) gene, with respect to polymyxa graminis transmitted bammv. this is confirmed by sequence comparison with rna2 of a fungus transmitted bammv isolate, which reveals the presence of a single deletion located in the 3'-terminal part o ... | 1995 | 7710357 |
| an analysis of spontaneous deletion sites in soil-borne wheat mosaic virus rna2. | plants mechanically inoculated with soil-borne wheat mosaic virus (sbwmv, oklahoma isolate) generated several deleted forms of rna2, the smaller genomic rna (3593 nt). four naturally deleted forms were cloned by rt-pcr methods and the sequences around each deletion site were compared. sbwmv rna2 molecules were deleted for 519, 759, 964, or 1030 nt, respectively, each within the coat protein-readthrough domain. no common sequences were found flanking the 5'- and 3'-sites of deletion; however, the ... | 1995 | 7747472 |
| complete nucleotide sequence of peanut clump virus rna 1 and relationships with other fungus-transmitted rod-shaped viruses. | the complete nucleotide sequence of rna 1 of the tentative furovirus peanut clump virus (pcv) has been determined by characterization of cloned cdna and by direct rna sequencing. the sequence is 5897 nucleotides in length and contains three long open reading frames (orfs). the 5'-terminal proximal orf has the potential to encode a polypeptide of m(r) 130942 (p131) containing methyltransferase and rna helicase homologous domains and displaying homology with large nonstructural proteins of alpha-l ... | 1994 | 7964624 |
| the nucleotide sequence of potato mop-top virus rna 2: a novel type of genome organization for a furovirus. | particles of isolate t of potato mop-top furovirus (pmtv) contain three rna species (6.5, 3.0 and 2.5 kb). hybridization tests with cloned cdna probes showed that none of these species was derived from another. rna 2 (2962 nt), which was sequenced, has non-coding regions of 368 nt and 285 nt at the 5' end and 3' end, respectively. near the 5' terminus, nucleotides 46 to 110 are able to form a stem-loop structure, the stem of which has 23 bp with only one mismatch and one unpaired nucleotide. fro ... | 1994 | 7996148 |
| detection and sequence analysis of a spontaneous deletion mutant of soil-borne wheat mosaic virus rna2 associated with increased symptom severity. | repeated passage of soil-borne wheat mosaic virus (sbwmv; wild-type oklahoma field isolate; designated okl-0) by manual inoculation resulted in deletion of part of sbwmv rna2. deletion was apparent in the population of rna2 molecules after only 11 weeks in primary inoculated wheat plants (called okl-1) and after 5 passages (okl-5; 20 weeks) no full-length rna2 remained. the extent of deletion in the oklahoma isolate was compared with that in the previously studied lab 1 (nebraska) isolate. rna2 ... | 1994 | 8030253 |
| complete nucleotide sequence and organization of the bipartite rna genome of soil-borne wheat mosaic virus. | the complete nucleotide sequences of rnas 1 and 2 of soil-borne wheat mosaic virus (sbwmv), type member of the furovirus group, were determined. rna 1 is 7099 nucleotides (nt) and encodes a 150-kda protein from the 5' end region, the uga termination codon of which can be partially read through to produce a 209-kda protein, and a 37-kda protein in the 3' end region. the c-terminal region of the 150-kda protein contains an ntp-binding helicase motif and the readthrough region, an rna polymerase mo ... | 1993 | 8317092 |
| sequence analysis of wheat and oat furovirus capsid protein genes suggests that oat golden stripe virus is a strain of soil-borne wheat mosaic virus. | in northern blots, cdna probes prepared to soil-borne wheat mosaic virus (sbwmv) rna-1 and rna-2 hybridized to rna-1 and rna-2, respectively, from a uk isolate of oat golden stripe virus (ogsv), as well as to their homologous rnas. rt-pcr was used to amplify, clone and sequence a region of about 750 nucleotides spanning the capsid protein gene and part of the readthrough protein on rna-2 from ogsv, a french isolate of sbwmv and two stable deletion mutants (lab1 and okl-7) of sbwmv isolates from ... | 1996 | 8738177 |
| non-aug translation initiation in a plant rna virus: a forty-amino-acid extension is added to the n terminus of the soil-borne wheat mosaic virus capsid protein. | rna 2 of soil-borne wheat mosaic virus (sbwmv), the type species of the genus furovirus, encodes a protein previously hypothesized to be initiated at an in-frame non-aug codon upstream of the aug initiation codon (nucleotide positions 334 to 336) for the 19-kda capsid protein. site-directed mutagenesis and in vitro transcription and translation analysis indicated that cug (nucleotides 214 to 216) is the initiation codon for a protein with a calculated molecular mass of 25 kda composed of a 40-am ... | 1998 | 9445077 |
| the nucleotide sequence of rna-1 of indian peanut clump furovius. | the nucleotide sequence of rna-1 of an isolate of the h serotype of indian peanut clump virus (ipcv-h) was shown to comprise 5,841 nucleotides. the rna contains three open reading frames (orf) which are between nucleotides 133 and 3,522, nucleotides 3,526 and 5,103 (assuming expression by suppression of the orf 1 termination codon) and nucleotides 5,168 and 5,539. the encoded polypeptides have m(r), of 129,687 (p130), 60,188 (p60) and 14,281 (p14). orf 2 is thought to be expressed by suppression ... | 1996 | 9526538 |
| transfer rna mimicry in a new group of positive-strand rna plant viruses, the furoviruses: differential aminoacylation between the rna components of one genome. | recent sequencing of the genomes of several furoviruses--fungus-transmitted rod-shaped positive-strand plant viruses--has suggested the presence of trna-like structures (tlss) at the 3' ends of the genomic rnas. we show here that the genomic rnas of soil-borne wheat mosaic virus (sbwmv), beet soil-borne virus (bsbv), potato mop-top virus (pmtv), peanut clump virus (pcv), and indian peanut clump virus (ipcv) all possess functional tlss that are capable of high-efficiency valylation. while the sbw ... | 1998 | 9657004 |
| genome sequences of poa semilatent and lychnis ringspot hordeiviruses. | the nucleotide sequences of the genomic rnas of two hordeiviruses, poa semilatent virus (pslv) and lychnis ringspot virus (lrsv), were determined. the genome organization of both viruses is similar to that of the type hordeivirus, barley stripe mosaic virus (bsmv). comparisons of the amino acid sequences of the hordeivirus replicase components revealed a high level of sequence similarity to those of soil-borne wheat mosaic virus (sbwmv), peanut clump virus (pcv), and indian peanut clump virus. t ... | 1998 | 9722881 |
| evolution and origins of tobamoviruses. | more than a dozen tobamoviruses are known. in nature, each species probably survives by moving between several closely related host species. each infected plant contains a population of variants, but in most host populations the tobamovirus population is stable. the phylogenetic relationships of tobamovirus species broadly correlate with those of their angiosperm hosts. the simplest explanation for this correlation is that they have coevolved with the angiosperms, and hence, like them, are about ... | 1999 | 10212939 |
| complete sequence and genome properties of chinese wheat mosaic virus, a new furovirus from china. | the complete nucleotide sequence of a virus infecting winter wheat in shandong province, china has been determined. this was previously thought to be soil-borne wheat mosaic virus but, while the two viruses are related, they are only 75% (rna1) and 63% (rna2) identical at the nucleotide level, while the amino acid sequences share from 62% (19 kda rna2 product) to 84% (rna1 replicase) identity. the analysis shows that the chinese virus should be considered a new member of the genus furovirus and ... | 1999 | 10355760 |
| sequences of european wheat mosaic virus and oat golden stripe virus and genome analysis of the genus furovirus. | the complete nucleotide sequences of both rnas of oat golden stripe virus (ogsv) and a wheat-infecting furovirus isolate from france, previously thought to be soil-borne wheat mosaic virus (sbwmv), have been determined. both viruses had a similar genomic organisation to sbwmv and chinese wheat mosaic virus, the two other furoviruses previously sequenced but had <70% nucleotides identical to them. the french isolate has been named european wheat mosaic virus (ewmv). phylogenetic analyses supporte ... | 1999 | 10497118 |
| complete sequence of rna 1 and the presence of trna-like structures in all rnas of potato mop-top virus, genus pomovirus. | the complete nucleotide sequence (6043 nt) of rna 1 from potato mop-top virus (pmtv-sw), the type member of the genus pomovirus, was determined. the first (5'-terminal) open reading frame (orf 1) encodes a predicted protein of 148 kda. orf 2 extends through the opal stop codon of orf 1 producing a predicted readthrough protein of 206 kda which resembles the rna-dependent rna polymerases (rdrp) of other fungal-transmitted viruses. it includes a methyltransferase, a helicase and a gdd rdrp motif, ... | 1999 | 10573175 |
| similarity and divergence among viruses in the genus furovirus. | nucleotide sequences of rnas 1 and 2 of a japanese strain of soil-borne wheat mosaic virus (sbwmv), the type species of the genus furovirus, and sorghum chlorotic spot virus (scsv) were determined from cloned cdna. the relationship among the japanese and us strains of sbwmv, scsv, oat golden stripe virus (ogsv), and recently proposed chinese wheat mosaic and european wheat mosaic viruses (cwmv and ewmv) were examined at the nucleotide and amino acid levels. pairwise comparisons of genome-encoded ... | 2000 | 10772992 |
| construction of full-length cdna clones to soil-borne wheat mosaic virus rna1 and rna2, from which infectious rnas are transcribed in vitro: virion formation and systemic infection without expression of the n-terminal and c-terminal extensions to the capsid protein. | the 19-kda capsid protein (cp) of soil-borne wheat mosaic furovirus (sbwmv) is encoded in the 5'-terminal region of rna2. in addition to cp, two cp-related proteins are translated from sbwmv rna2: (1) a 24-kda protein (n-cp) with an n-terminal 40-amino-acid extension initiated at an upstream in-frame cug codon; and (2) an 83-kda protein (cp-rt) with an about 580-amino-acid, c-terminal extension by partial translational readthrough at the uga termination codon at the end of the cp gene. we examin ... | 2000 | 11062037 |
| a new york isolate of soil-borne wheat mosaic virus differs considerably from the nebraska type strain in the nucleotide sequences of various coding regions but not in the deduced amino acid sequences. | a wheat-infecting furovirus found in tompkins county, new york, u.s.a. was identified as a strain of soil-borne wheat mosaic virus (sbwmv) by means of sequence analyses of portions of its rna 1 and 2. the nucleotide sequences of several of its genes differed by c. 9 to 12% from those of the corresponding genome regions of the nebraska type strain of sbwmv. the deduced amino acid sequences of the putative translation products, however, suggested much closer relationships. thus, the amino acid seq ... | 2002 | 11958460 |
| reassortment between genetically distinct japanese and us strains of soil-borne wheat mosaic virus: rna1 from a japanese strain and rna2 from a us strain make a pseudorecombinant virus. | soil-borne wheat mosaic virus (sbwmv), the type species of the genus furovirus, has a plus-sense bipartite rna genome. japanese and us strains of sbwmv are genetically distantly related, despite their biologically identical properties. here we report formation of a pseudorecombinant virus consisting of rna1 from a japanese strain and rna2 from a us strain, using infectious in vitro transcripts for both strains. full-length infectious cdna clones for a japanese strain were previously constructed ... | 2002 | 12111425 |
| functional replacement of the tobacco rattle virus cysteine-rich protein by pathogenicity proteins from unrelated plant viruses. | mutation of the 16k gene encoded by rna1 of tobacco rattle virus (trv) greatly reduced the levels of viral rna that accumulated in both infected protoplasts and plants, showing that the 16k cysteine-rich protein (crp) is required for efficient multiplication of trv. overexpression of the 16k protein, either from an additional copy of the gene carried on trv rna2 or from a pvx vector, led to an increase in the severity of disease symptoms, suggesting that the protein has a role in the pathogenici ... | 2002 | 12127786 |
| the optimal temperature for rna replication in cells infected by soil-borne wheat mosaic virus is 17 degrees c. | systemic infection of wheat plants with soil-borne wheat mosaic virus (sbwmv) requires temperatures below 20 degrees c. here we examine the cause of the temperature sensitivity by inoculating infectious in vitro transcripts of sbwmv rna1 and rna2 to barley mesophyll protoplasts. after rna inoculation, protoplasts were incubated at temperatures between 15 and 25 degrees c for up to 48 h. western blot analysis showed that the capsid protein accumulated most abundantly at 17 degrees c but was not d ... | 2003 | 12655102 |
| evidence that the 37 kda protein of soil-borne wheat mosaic virus is a virus movement protein. | experiments were conducted to determine if the 37 kda protein (37k) of soil-borne wheat mosaic virus (sbwmv) is a virus movement protein. first, evidence was obtained that indicated that 37k has the ability to move from cell to cell, similar to other virus movement proteins (mps). plasmids containing the gfp gene fused to the sbwmv 37k, the coat protein (cp) or the cp readthrough domain (rt) orfs were delivered by biolistic bombardment to wheat and tobacco leaves. in wheat leaves, cell-to-cell m ... | 2003 | 14573821 |
| stable deletions arising in the readthrough region of soil-borne wheat mosaic virus rna2 define the 5' limit of the functional promoter for the p19 subgenomic rna. | the appearance of de novo deletion mutations in the readthrough (rt) region (nucleotide positions 861-2591) downstream of the capsid protein (cp) gene of a japanese strain of soil-borne wheat mosaic virus rna2 was examined using infectious transcripts. mutant rna2s with different deletions predominated in independent serial passage experiments but all best-adapted mutants retained the 3'-terminal portion of the rt gene in frame with the cp gene. the longest best-adapted mutation deleted the 1434 ... | 2005 | 15824885 |
| development of a multiplexed pcr detection method for barley and cereal yellow dwarf viruses, wheat spindle streak virus, wheat streak mosaic virus and soil-borne wheat mosaic virus. | barley and cereal yellow dwarf viruses (b/cydvs), wheat spindle streak mosaic (wssmv), soil-borne wheat mosaic virus (sbwmv) and wheat streak mosaic virus (wsmv) constitute the most economically important group of wheat viruses. in this paper, a multiplex reverse transcription polymerase chain reaction (m-rt-pcr) method was developed for the simultaneous detection and discrimination of eight viruses: five strains of b/cydvs, wssmv, sbwmv and wsmv. the protocol uses specific primer sets for each ... | 2008 | 18063125 |
| cell-free translation of soil-borne wheat mosaic virus rnas. | the genome of soil-borne wheat mosaic (sbwmv) virus appears to be composed of two rnas. three strains have the same large rna, designated 1.0l rna, but differ in the size of the smaller rna, designated 0.5l rna for wild type (wt), 0.4l rna for mutant lab 2, and 0.35l rna for mutant lab 1, where l corresponds to approximately 6700 nucleotide residues. the major translation products of 1.0l rna in rabbit reticulocyte lysates had apparent molecular weights of 180,000 (180k), 152k, 135k, 80k, and 45 ... | 1985 | 18639852 |
| sequence analysis of a soil-borne wheat mosaic virus isolate from italy shows that it is the same virus as european wheat mosaic virus and soil-borne rye mosaic virus. | the complete sequence of the two rnas of a furovirus isolate from durum wheat in italy was determined. sequence comparisons and phylogenetic analysis were done to compare the italian virus with soil-borne wheat mosaic virus (sbwmv) from the usa and with furovirus sequences recently published as european wheat mosaic virus (ewmv), from wheat in france, and soil-borne rye mosaic virus (sbrmv), from rye and wheat in germany. over the entire genome, the italian isolate rna1 and rna2 had respectively ... | 2001 | 18726440 |
| soil-borne viruses affecting cereals: known for long but still a threat. | a number of furo- and bymoviruses, which are naturally transmitted by the ubiquitous soil-borne protozoon polymyxa graminis are of great economic importance for autumn-sown wheat, barley, rye, triticale, and oats. the two barley-affecting bymoviruses, barley yellow mosaic virus and barley mild mosaic virus, are widespread in europe and east asia. production of wheat, rye, and triticale is threatened by three related furoviruses and two bymoviruses. soil-borne wheat mosaic virus mainly occurs in ... | 2009 | 19159654 |
| estimation of the size of genetic bottlenecks in cell-to-cell movement of soil-borne wheat mosaic virus and the possible role of the bottlenecks in speeding up selection of variations in trans-acting genes or elements. | genetic bottlenecks facilitate the fixation and extinction of variants in populations, and viral populations are no exception to this theory. to examine the existence of genetic bottlenecks in cell-to-cell movement of plant rna viruses, we prepared constructs for soil-borne wheat mosaic virus rna2 vectors carrying two different fluorescent proteins, yellow fluorescent protein (yfp) and cyan fluorescent protein (cfp). coinoculation of host plant leaves with the two rna2 vectors and the wild-type ... | 2010 | 19955302 |
| application of sequence-independent amplification (sia) for the identification of rna viruses in bioenergy crops. | miscanthus x giganteus, energycane, and panicum virgatum (switchgrass) are three potential biomass crops being evaluated for commercial cellulosic ethanol production. viral diseases are potentially significant threats to these crops. therefore, identification of viruses infecting these bioenergy crops is important for quarantine purposes, virus resistance breeding, and production of virus-free planting materials. the application is described of sequence-independent amplification, for the identif ... | 2010 | 20638415 |
| differential detection of wheat yellow mosaic virus, japanese soil-borne wheat mosaic virus and chinese wheat mosaic virus by reverse transcription loop-mediated isothermal amplification reaction. | a differential detection method for three wheat viruses: wheat yellow mosaic virus (wymv), japanese soil-borne mosaic virus (jsbwmv) and chinese wheat mosaic virus (cwmv) using reverse transcription loop-mediated isothermal amplification (rt-lamp) reaction was developed. all three primer sets, which were designed from the genome sequences of wymv, jsbwmv and cwmv respectively, worked most efficiently at 65 °c and could detect each virus rna within 10 min by fluorescence monitoring using an isoth ... | 2013 | 23523736 |
| soil-borne wheat mosaic virus infectious clone and manipulation for gene-carrying capacity. | a full-length infectious cdna clone of soil-borne wheat mosaic virus (sbwmv; genus furovirus; family virgaviridae) was developed for agrobacterium delivery. the cloned virus can be agroinfiltrated to nicotiana benthamiana for subsequent infection of wheat (triticum aestivum, l.). the utility of the virus as a vector for gene silencing and expression was assessed through sequence insertions in multiple sites of rna2. virus-induced photobleaching was observed in n. benthamiana but not in wheat, de ... | 2016 | 27236459 |
| detection and discrimination of european isolates of soil-borne wheat mosaic virus using immunocapture real-time reverse transcription-polymerase chain reaction. | soil-borne wheat mosaic virus (sbwmv) is a viral pathogen of cereal crops. although first detected in the united states, it has since been reported in germany and poland. to date, two sbwmv strains (nebraska-like and new-york-like types) have been detected. while the nucleotide sequences of the coat protein (cp) genes from the two strains show only 89% similarity, the deduced amino acid sequences of this region are identical. therefore, the isolates cannot be differentiated serologically using t ... | 2015 | 26384757 |
| genome-wide association analysis identified snps closely linked to a gene resistant to soil-borne wheat mosaic virus. | using association and linkage mapping, two snp markers closely linked to the sbwmv resistance gene on chromosome 5d were identified and can be used to select the gene in breeding. soil-borne wheat mosaic virus (sbwmv) disease is a serious viral disease of winter wheat growing areas worldwide. sbwmv infection can significantly reduce grain yield up to 80 %. developing resistant wheat cultivars is the only feasible strategy to reduce the losses. in this study, wheat infinium iselect beadchips with ... | 2014 | 24522724 |