| characterization of bacteriophage bfk20 from brevibacterium flavum. | bacteriophage bfk20 was isolated from a brevibacterium flavum strain that had become contaminated during industrial fermentation. bfk20 has a polyhedral head 50 nm wide and a non-contractile tail 200 nm long and 10 nm in diameter. the genome of this bacteriophage consists of a linear double stranded dna molecule of 44-45 kb with cohesive ends. the capsid of phage bfk20 contains nine polypeptides with molecular masses from 22.0-108.0 kda. bfk20 dna was used as a donor for fragments carrying promo ... | 1992 | 1512569 |
| complete nucleotide sequence and genome analysis of bacteriophage bfk20--a lytic phage of the industrial producer brevibacterium flavum. | the entire double-stranded dna genome of bacteriophage bfk20, a lytic phage of the brevibacterium flavum ccm 251--industrial producer of l-lysine--was sequenced and analyzed. it consists of 42,968 base pairs with an overall molar g + c content of 56.2%. fifty-five potential open reading frames were identified and annotated using various bioinformatics tools. clusters of functionally related putative genes were defined (structural, lytic, replication and regulatory). to verify the annotation of s ... | 2006 | 16457869 |
| transcriptional profiling of bacteriophage bfk20: coexpression interrogated by "guilt-by-association" algorithm. | global gene expression profiling of bacteriophage bfk20 infecting the industrial l-lysine producer brevibacterium flavum ccm 251 was performed using dna microarray. the relative gene expressions were measured in fourteen time samples collected during phage development. phage genes were classified as early, middle, late or unassigned based on complex expression patterns during infection. temporal classification of bfk20 genes was in concordance with previous predictions. however, proposed late re ... | 2007 | 17052739 |
| endolysin of bacteriophage bfk20: evidence of a catalytic and a cell wall binding domain. | a gene product of orf24' was identified on the genome of corynephage bfk20 as a putative phage endolysin. the protein of endolysin bfk20 (gp24') has a modular structure consisting of an n-terminal amidase_2 domain (gp24cd) and a c-terminal cell wall binding domain (gp24bd). the c-terminal domain is unrelated to any of the known cell wall binding domains of phage endolysins. the whole endolysin gene and the sequences of its n-terminal and c-terminal domains were cloned; proteins were expressed in ... | 2011 | 21592196 |