| genome characterization and taxonomy of plantago asiatica mosaic potexvirus. | the complete nucleotide sequence of plantago asiatica mosaic virus (p1amv) genomic rna has been determined. the 6128 nucleotide sequence contains five open reading frames (orfs) coding for proteins of m(r) 156k (orf1), 25k (orf2), 12k (orf3), 13k (orf4) and 22k (orf5). the sequences of these p1amv proteins exhibit strong homology to the proteins of the other potexviruses. phylogenetic trees based on the multiple sequence alignments of three conserved domains in orf1 product and capsid protein re ... | 1994 | 8113746 |
| evidence for two nonoverlapping functional domains in the potato virus x 25k movement protein. | to study subdomain organization of the potato virus x (pvx) movement protein (mp) encoded by the first gene in the triple gene block (tgb), we mutated the 25-kda tgbp1 protein. the n-terminal deletion of the helicase motifs i, ia, and ii resulted in loss of the atpase activity and rna binding. a frameshift mutation truncating the c-terminal motifs v and vi gave rise to increase of the tgbp1 atpase activity and had little effect on rna binding in vitro. fusions of the green fluorescent protein wi ... | 1999 | 10405356 |
| complete nucleotide sequence of tulip virus x (tvx-j): the border between species and strains within the genus potexvirus. | the complete nucleotide sequence of the genomic rna of tulip virus x japanese isolate (tvx-j) has been determined. the sequence is 6056 nucleotides in length, excluding the poly(a) tail at the 3' terminus, and contains five open reading frames (orfs) coding for proteins of mr 153, 25, 12, 10, and 22 kda (orfs 1 through 5, respectively). the genome organization of tvx-j is similar to that of potexviruses, and the encoded proteins share a high degree of homology to the corresponding proteins of ot ... | 2001 | 11811681 |
| complete nucleotide sequence and genome organization of a cactus virus x strain from hylocereus undatus (cactaceae). | the complete nucleotide sequence of a strain of cactus virus x (cvx-hu) isolated from hylocereus undatus (cactaceae) has been determined. excluding the poly(a) tail, the sequence is 6614 nucleotides in length and contains seven open reading frames (orfs). the genome organization of cvx is similar to that of other potexviruses. orf1 encodes the putative viral replicase with conserved methyltransferase, helicase, and polymerase motifs. within orf1, two other orfs were located separately in the +2 ... | 2004 | 15098117 |
| nucleotide sequence analysis of seven japanese isolates of plantago asiatica mosaic virus (plamv): a unique potexvirus with significantly high genomic and biological variability within the species. | the genomic sequences of plantago asiatica mosaic virus (plamv), six lily isolates and one primrose isolate from japan, were determined. the genomic size of all isolates was 6102 nucleotides, containing the five open reading frames typical of members of the genus potexvirus. pairwise comparison analyses confirmed the close relationship between plamv and tulip virus x. however, quite low identities were observed between different plamv isolates, including foreign isolates; nucleotide sequence ide ... | 2008 | 17987351 |
| a simple, sensitive, specific detection of mixed infection of multiple plant viruses using macroarray and microtube hybridization. | a simple, sensitive and specific method using a cdna macroarray to detect multiple viruses was devised. the method is used in plants such as potato and lily, which need a reliable routine diagnosis for mixed infection. the biotinylated crna targets were prepared using an in vitro transcription-based system that was designed especially to eliminate nonspecific hybridizations. the macroarray hybridization was carried out using a convenient, cost-effective "microtube hybridization" (mth) system. by ... | 2008 | 18760308 |
| variability in the level of rna silencing suppression caused by triple gene block protein 1 (tgbp1) from various potexviruses during infection. | rna silencing is an important defence mechanism against virus infection, and many plant viruses encode rna silencing suppressors as a counter defence. in this study, we analysed the rna silencing suppression ability of multiple virus species of the genus potexvirus. nicotiana benthamiana plants exhibiting rna silencing of a green fluorescent protein (gfp) transgene showed reversal of gfp fluorescence when systemically infected with potexviruses. however, the degree of gfp fluorescence varied amo ... | 2009 | 19264652 |
| viral-induced systemic necrosis in plants involves both programmed cell death and the inhibition of viral multiplication, which are regulated by independent pathways. | resistant plants respond rapidly to invading avirulent plant viruses by triggering a hypersensitive response (hr). an hr is accompanied by a restraint of virus multiplication and programmed cell death (pcd), both of which have been observed in systemic necrosis triggered by a successful viral infection. here, we analyzed signaling pathways underlying the hr in resistance genotype plants and those leading to systemic necrosis. we show that systemic necrosis in nicotiana benthamiana, induced by pl ... | 2010 | 20121450 |
| phylogeny, coat protein genetic variability, and transmission via seeds of hosta virus x. | the complete genome of hosta virus x (hvx), which is thought to be a distinct species of potexvirus, was sequenced. nucleotide sequences of hvx were compared with those of other members of the genus potexvirus and phylogenetic tree was constructed. the range of identities of viral replicase open reading frame 1 (orf1) between hvx and other potexviruses were 43.1%-55.1% and 35.9%-46.6% at the nucleotide and amino acid levels, respectively. phylogenetic analysis was performed according to the amin ... | 2012 | 22453807 |
| efficient foreign gene expression in planta using a plantago asiatica mosaic virus-based vector achieved by the strong rna-silencing suppressor activity of tgbp1. | plant virus expression vectors provide a powerful tool for basic research as well as for practical applications. here, we report the construction of an expression vector based on plantago asiatica mosaic virus (plamv), a member of the genus potexvirus. modification of a vector to enhance the expression of a foreign gene, combined with the use of the foot-and-mouth disease virus 2a peptide, allowed efficient expression of the foreign gene in two model plant species, arabidopsis thaliana and nicot ... | 2014 | 24154949 |
| the ire1/bzip60 pathway and bax inhibitor 1 suppress systemic accumulation of potyviruses and potexviruses in arabidopsis and nicotiana benthamiana plants. | the inositol requiring enzyme (ire1) is an endoplasmic reticulum (er) stress sensor. when activated, it splices the bzip60 mrna, producing a truncated transcription factor that upregulates genes involved in the unfolded protein response. bax inhibitor 1 (bi-1) is another er stress sensor that regulates cell death in response to environmental assaults. the potyvirus 6k2 and potexvirus tgb3 proteins are known to reside in the er, serving, respectively, as anchors for the viral replicase and moveme ... | 2016 | 27578623 |
| lectin-mediated resistance impairs plant virus infection at the cellular level. | plants possess a multilayered defense response, known as plant innate immunity, to infection by a wide variety of pathogens. lectins, sugar binding proteins, play essential roles in the innate immunity of animal cells, but the role of lectins in plant defense is not clear. this study analyzed the resistance of certain arabidopsis thaliana ecotypes to a potexvirus, plantago asiatica mosaic virus (plamv). map-based positional cloning revealed that the lectin gene jacalin-type lectin required for p ... | 2012 | 22307853 |
| deficiency of the eif4e isoform ncbp limits the cell-to-cell movement of a plant virus encoding triple-gene-block proteins in arabidopsis thaliana. | one of the important antiviral genetic strategies used in crop breeding is recessive resistance. two eukaryotic translation initiation factor 4e family genes, eif4e and eifiso4e, are the most common recessive resistance genes whose absence inhibits infection by plant viruses in potyviridae, carmovirus, and cucumovirus. here, we show that another eif4e family gene, ncbp, acts as a novel recessive resistance gene in arabidopsis thaliana toward plant viruses in alpha- and betaflexiviridae. we found ... | 2017 | 28059075 |
| exa1, a gyf domain protein, is responsible for loss-of-susceptibility to plantago asiatica mosaic virus in arabidopsis thaliana. | one of the plant host resistance machineries to viruses is attributed to recessive alleles of genes encoding critical host factors for virus infection. this type of resistance, also referred to as recessive resistance, is useful for revealing plant-virus interactions and for breeding antivirus resistance in crop plants. therefore, it is important to identify a novel host factor responsible for robust recessive resistance to plant viruses. here, we identified a mutant from an ethylmethane sulfona ... | 2016 | 27402258 |
| in planta recognition of a double-stranded rna synthesis protein complex by a potexviral rna silencing suppressor. | rna silencing plays an important antiviral role in plants and invertebrates. to counteract antiviral rna silencing, most plant viruses have evolved viral suppressors of rna silencing (vsrs). triple gene block protein1 (tgbp1) of potexviruses is a well-characterized vsr, but the detailed mechanism by which it suppresses rna silencing remains unclear. we demonstrate that transgenic expression of tgbp1 of plantago asiatica mosaic virus (plamv) induced developmental abnormalities in arabidopsis thal ... | 2014 | 24879427 |
| molecular detection and characterization of a divergent isolate of plantago asiatica mosaic virus in plantago asiatica. | four samples of plantago asiatica showing mottle or mottled yellowing symptoms were collected and three of them were confirmed to be infected with plantago asiatica mosaic virus (plamv) by illumina hiseq 2500 paired-end rna sequencing. consequently, the complete genomic sequence of a korean isolate of plamv (plamv isolate gunwi) from p. asiatica was determined. the nucleotide sequence of plamv-gunwi was most closely related to a russian isolate of plamv (plamv-ru) collected from p. asiatica but ... | 2016 | 28466044 |
| antiviral defense involves ago4 in an arabidopsis-potexvirus interaction. | in plants, rna silencing regulates gene expression through the action of dicer-like (dcl) and argonaute (ago) proteins via micro rnas and rna-dependent dna methylation (rddm). in addition, rna silencing functions as an antiviral defense mechanism by targeting virus-derived double-stranded rna. plants encode multiple ago proteins with specialized functions, including ago4-like proteins that affect rddm and ago2, ago5, and ago1, which have antiviral activities. here, we show that ago4 is also requ ... | 2016 | 27762650 |
| complete genome sequences of two highly divergent japanese isolates of plantago asiatica mosaic virus. | plantago asiatica mosaic virus (plamv) is a member of the genus potexvirus and has an exceptionally wide host range. it causes severe damage to lilies. here we report on the complete nucleotide sequences of two new japanese plamv isolates, one from the eudicot weed viola grypoceras (plamv-vi), and the other from the eudicot shrub nandina domestica thunb. (plamv-nj). their genomes contain five open reading frames (orfs), which is characteristic of potexviruses. surprisingly, the isolates showed o ... | 2017 | 27743255 |
| a necrosis-inducing elicitor domain encoded by both symptomatic and asymptomatic plantago asiatica mosaic virus isolates, whose expression is modulated by virus replication. | systemic necrosis is the most destructive symptom induced by plant pathogens. we previously identified amino acid 1154, in the polymerase domain (pol) of rna-dependent rna polymerase (rdrp) of plantago asiatica mosaic virus (plamv), which affects plamv-induced systemic necrosis in nicotiana benthamiana. by point-mutation analysis, we show that amino acid 1,154 alone is not sufficient for induction of necrotic symptoms. however, plamv replicons that can express only rdrp, derived from a necrosis- ... | 2011 | 21190438 |
| the n-terminal region of the plantago asiatica mosaic virus coat protein is required for cell-to-cell movement but is dispensable for virion assembly. | potexvirus cell-to-cell movement requires coat protein (cp) and movement proteins. in this study, mutations in two conserved in-frame aug codons in the 5' region of the cp open reading frame of plantago asiatica mosaic virus (plamv) were introduced, and virus accumulation of these mutants was analyzed in inoculated and upper noninoculated leaves. when cp was translated only from the second aug codon, virus accumulation in inoculated leaves was lower than that of wild-type plamv, and the viral sp ... | 2009 | 19445592 |
| a single amino acid in the rna-dependent rna polymerase of plantago asiatica mosaic virus contributes to systemic necrosis. | from a lily isolate of plantago asiatica mosaic virus (plamv-li), two sub-isolates (li1 and li6) were obtained. although the nucleotide sequences of li1 and li6 were highly conserved, they showed different pathogenicity in nicotiana benthamiana. li1 caused necrosis, whereas li6 infected the plant asymptomatically. inoculation tests with chimeric and point-mutated viruses revealed that amino acid 1154 of the rna-dependent rna polymerase (rdrp) contributes to the necrotic symptoms. the accumulatio ... | 2006 | 16633739 |
| [primary structure of the triple block rna genes of the plantago asiatica mosaic virus]. | | 1993 | 8477237 |