| serological and biological relationships among viruses in the bean common mosaic virus subgroup. | bean common mosaic virus (bcmv), blackeye cowpea mosaic virus (b1cmv), cowpea aphid-borne mosaic virus (cabmv), azuki bean mosaic virus (azmv), and peanut stripe virus (pstv) are five species of the genus potyvirus, family potyviridae which are seed-transmitted in beans or cowpeas. eighteen isolates of bcmv, five isolates of b1cmv, four isolates of cabmv, and one isolate each of azmv, and pstv were compared serologically using a panel of 13 monoclonal antibodies (mabs) raised against bcmv, b1cmv ... | 1992 | 1450766 |
| strains of bean common mosaic virus consist of at least two distinct potyviruses. | bean common mosaic virus (bcmv) consists of a large number of pathotypes and strains which have largely been identified by their characteristic interactions with a selected number of differential bean cultivars. the relationships among these strains and other potyviruses that infect legumes are complex, with indications that bcmv, blackeye cowpea mosaic virus (blcmv) and azuki bean mosaic virus (azmv) may be strains of the one virus. using high performance liquid chromatographic peptide profiles ... | 1992 | 1450767 |
| coat protein properties suggest that azuki bean mosaic virus, blackeye cowpea mosaic virus, peanut stripe virus, and three isolates from soybean are all strains of the same potyvirus. | the interrelationship of a number of potyviruses infecting legumes has been investigated by comparing molecular properties of their coat proteins. comparison of the coat proteins by the techniques of amino acid analysis and page was inadequate to distinguish strains from distinct potyviruses. however, high-performance liquid chromatographic peptide profiles of tryptic digests of coat proteins of these legume-infecting potyviruses enabled such assignments to be made. these data indicate that amin ... | 1992 | 1500273 |
| coat protein of potyviruses. 7. amino acid sequence of peanut stripe virus. | the amino acid sequence of the 287-residue coat protein of peanut stripe virus (pstv) was determined from the sequences of overlapping peptide fragments. results indicated that the amino terminus was blocked by an acetyl group, as has previously been found for the coat protein of johnsongrass mosaic potyvirus. comparison of the pstv sequence with coat proteins of 20 distinct potyviruses gave sequence identities of 47-57%, except for zucchini yellow mosaic virus (zymv), passionfruit woodiness vir ... | 1991 | 1863222 |
| cloning of the capsid protein gene from a blotch isolate of peanut stripe virus. | the 3' terminal 1,367 nucleotides (nts) of a blotch isolate of the potyvirus, peanut stripe virus (pstv), were cloned and sequenced. this region included the 861 nts (287 amino acids) of the pstv capsid protein (cp). the amino acid sequence of the predicted proteinase cleavage site was identified. this region shared limited homology with other potyvirus proteinase cleavage sites. the viral cp gene sequence was followed by 254 nucleotides of 3' nontranslated sequence and a poly-a tail. based on c ... | 1993 | 7916587 |
| cloning and sequence analysis of the coat protein genes of an australian strain of peanut mottle and an indonesian 'blotch' strain of peanut stripe potyviruses. | we have analysed the coat protein gene sequences of two potyviruses infecting peanut. the 3' terminal 1247 nucleotides (nt) of an australian strain of peanut mottle virus (pemov-au) and the 3' terminal 1388 nt of an indonesian 'blotch' strain of peanut stripe virus (pstv-ib) were cloned and sequenced. those regions included the 861 and 864 nt encoding the respective putative coat proteins as well as the 285 and 253 nt, respectively of 3' non-coding sequences. comparison of the nucleotide sequenc ... | 1994 | 7999143 |
| cowpea aphid borne mosaic virus-morocco and south african passiflora virus are strains of the same potyvirus. | high performance liquid chromatography (hplc) profiles of tryptic peptides and partial amino acid sequence analysis have been employed to establish the taxonomic status of the moroccan isolate of cowpea aphid-borne mosaic virus (cabmv). some previous reports have suggested cabmv to be very closely related to blackeye cowpea mosaic virus (b1cmv) while other reports have concluded that this relationship is distant. in this report a tryptic digest of the coat protein of cabmv-morocco was compared w ... | 1994 | 8002788 |
| nucleotide sequence and genome organization of peanut stripe potyvirus. | a blotch isolate of peanut stripe virus (pstv) was cloned, sequenced and compared with other full-length potyvirus sequences. the viral genome was 10059 nucleotides (nt) in length excluding the poly(a) tail. two potential aug start codons were identified in the 5' non-translated region. analysis of in vitro translation products from transcripts containing the first 600 nt of the pstv genome indicated that the first aug (nt 134 to 136) was preferred over the second aug (nt 146 to 148) for initiat ... | 1994 | 8077957 |
| the cdna sequence and infectious transcripts of peanut stripe virus. | a full-length cdna clone of the blotch isolate of the peanut stripe potyvirus (pstv) rna genome was constructed downstream from the bacteriophage sp6 rna polymerase promoter. the full-length pstv cdna clone (pstvsf9) was sequenced and compared to the previously published sequence of pstv-b. in vitro-synthesized pstv transcripts capped with m7gpppg were infectious in nicotiana benthamiana plants, and the progeny virus was aphid transmissible. to confirm the origin of infection, a mutant pstv (pst ... | 1996 | 8666291 |
| sequences of the coat protein gene of five peanut stripe virus (pstv) strains from thailand and their evolutionary relationship with other bean common mosaic virus sequences. | the coat protein gene and part of the 3' non-coding region of five strains of peanut stripe virus (pstv) from thailand have been cloned and sequenced. phylogenetic comparisons of these strains, known as t1, t3, t5, t6 and t7, and related sequences showed that these strains are indeed strains of pstv. further, pstv strains appear to be related to each other according to their geographic origin. that is, the thai strains are more closely related to each other than they are to strains from the usa ... | 1998 | 9787652 |
| potyvirus aphid transmission requires helper component and homologous coat protein for maximal efficiency. | aphid transmission of potyviruses depends on the presence of specific sequence domains in two virus encoded proteins, the coat protein (cp) and helper component-proteinase (hc-pro). aphid transmissable peanut stripe virus (pstv), like most potyviruses, has an asp-ala-gly (dag) motif in the amino-terminal part of the cp. peanut mottle virus (pemov) was determined to be highly aphid transmissible but has a unique asp-ala-ala-ala (daaa) motif. to determine if the daaa motif could functionally repla ... | 1998 | 9856099 |
| a mosaic disease of senna hirsuta induced by a potyvirus in nigeria. | a virus inducing mosaic and severe leaf malformation, isolated from senna hirsuta in nigeria, was studied. the virus had a rather narrow host range, infecting a few species in caesalpinaceae, chenopodiaceae and fabaceae families. the virus was widespread in southern nigeria with prevalence ranging from 74% to 86.4% in some locations. it was transmitted mechanically and in a non-persistent manner by myzuspersicae, aphis craccivora and a. spiraecola. there was no evidence of transmission by seeds. ... | 2001 | 11719985 |
| bean common mosaic virus isolates causing different symptoms in asparagus bean in china differ greatly in the 5'-parts of their genomes. | potyvirus isolates from asparagus bean ( vigna sesquipedalis) plants in zhejiang province, china, caused either rugose and vein banding mosaic symptoms (isolate r) or severe yellowing (isolate y) in this host, but were otherwise similar in host range. both isolates were completely sequenced and shown to be isolates of bean common mosaic virus (bcmv). the complete sequences were 9992 (r) or 10062 (y) nucleotides long and shared 91.7% identical nucleotides (93.2% identical amino acids) in their ge ... | 2002 | 12111434 |
| peanut stripe potyvirus resistance in peanut (arachis hypogaea l.) plants carrying viral coat protein gene sequences. | peanut (arachis hypogaea l.) lines exhibiting high levels of resistance to peanut stripe virus (pstv) were obtained following microprojectile bombardment of embryogenic callus derived from mature seeds. fertile plants of the commercial cultivars gajah and nc7 were regenerated following co-bombardment with the hygromycin resistance gene and one of two forms of the pstv coat protein (cp) gene, an untranslatable, full length sequence (cp2) or a translatable gene encoding a cp with an n-terminal tru ... | 2004 | 15070076 |
| the complete nucleotide sequence of a pakistani isolate of watermelon mosaic virus provides further insights into the taxonomic status in the bean common mosaic virus subgroup. | watermelon mosaic virus (wmv) is a potyvirus with a worldwide distribution, but is mostly found in temperate and mediterranean regions. the complete nucleotide (nt) sequence of a pakistani isolate of wmv (wmv-pk) was determined and compared with french isolate (wmv-fr) and other closely related potyviruses. wmv-pk showed overall identities of 94.4% (nt) and 96% (amino acid; aa) with the wmv-fr. however, variability was observed in the 5' utr and p1 region. although sequence identities over most ... | 2006 | 16732483 |
| molecular analysis of the coat protein gene of peanut stripe virus from china. | peanut stripe virus (pstv) is one of the most common viruses infecting peanut that causes great economic losses every year. the 3อด-terminal 1082 bp of 74 pstv isolates collected from 12 districts of shandong province, china were sequenced. their coat protein (cp) genes were 864 bp in length and shared identities of 98.0%~100% and 98.3% ~100% at nt and aa levels. the identities between the cp genes of these isolates and other 36 isolates from the genbank were 93.5%~100% and 92.0%~100% at nt and a ... | 2011 | 21692560 |