| inclusion bodies and tubular structures in chenopodium amaranticolor plants infected with strawberry latent ringspot virus. | | 1970 | 4192487 |
| two protein and two rna species in particles of strawberry latent ringspot virus. | | 1974 | 4843745 |
| single-step multiplex rt-pcr for simultaneous and colourimetric detection of six rna viruses in olive trees. | a single-step multiplex rt-pcr was developed for the simultaneous and colourimetric detection of six rna viruses (cucumber mosaic virus, cherry leaf roll virus, strawberry latent ringspot virus, arabis mosaic virus, olive latent-1 virus and olive latent-2 virus) which infect olive trees. six compatible primer set for one-step rt-pcr amplification in a single closed-tube and 3' digoxigenin labelled probes were designed in optimal, specific and conserved regions. the method has been assessed with ... | 2001 | 11516487 |
| occurrence of nepoviruses in rubus species in the czech republic. | the occurrence of arabis mosaic virus (amv), raspberry ringspot virus (rrv), tomato black ring virus (tbrv), strawberry latent ringspot virus (slrv) and cherry leaf roll virus (clrv) in cultivated and wild plants of raspberry and blackberry has been studied in the czech republic in 1993-1996. five hundred and seventy samples were collected at 51 localities and assayed by double antibody sandwich enzyme-linked immunosorbent assay (das-elisa). the results represent the first evidence on the occurr ... | 1997 | 9385408 |
| nucleotide sequence of the coat protein genes of citrus mosaic virus. | the sequence of the 3'-terminal 2,201 nucleotides of rna2 of citrus mosaic virus (cimv) was determined. the sequence contains a long open reading frame (orf) of 1989 nucleotides in the virus-sense strand and at 3' untranslated region of 212 nucleotides upstream of a poly(a) tail. the n-terminal amino acid sequence of the two coat proteins was determined by edman degradation and the corresponding coding regions were identified in the polyprotein. the larger coat protein with mr 48,122 is encoded ... | 1996 | 8806186 |
| sequence analysis and location of capsid proteins within rna 2 of strawberry latent ringspot virus. | the nucleotide sequence of the rna 2 of a strawberry isolate (h) of strawberry latent ringspot virus (slrsv) comprised 3824 nucleotides and contained one long open reading frame with a theoretical coding capacity of 890 amino acids equivalent to a protein of 98.8k. the n-terminal amino acid sequences of virion-derived proteins were determined by edman degradation allowing the capsid coding regions to be located and serine/glycine cleavage sites to be identified within the polyprotein. the amino ... | 1994 | 8077958 |
| nucleotide sequence of the coat protein genes of strawberry latent ringspot virus: lack of homology to the nepoviruses and comoviruses. | the sequence of the 3'-terminal 2424 nucleotides of rna-2 of the flowering cherry strain of strawberry latent ringspot virus (slrv) was determined from cdna clones. the sequence contains a reading frame in the virus-sense strand of 2070 nucleotides, a 3' untranslated region of 552 nucleotides and a 3'-terminal poly(a) tract. the positions of the two coat proteins of slrv within the reading frame were determined from sequence data obtained by n-terminal sequencing using edman degradation. the lar ... | 1994 | 8021614 |
| the nucleotide sequence of a satellite rna associated with strawberry latent ringspot virus. | the nucleotide sequence of a satellite rna associated with a strawberry isolate (h) of strawberry latent ringspot nepovirus (slrsv) was determined from cdna copies and the 5' end sequence was deduced from directly sequenced virion rna. at the 3' end a poly(a) sequence was identified. a long open reading frame encoding a polypeptide of 331 amino acids (m(r) 36488) was determined. sequence comparisons showed that slrsv satellite rna has no extensive homology with other sequences in the genembl and ... | 1993 | 7685374 |
| synthesis and proteolytic processing of arabis mosaic nepovirus, cherry leaf roll nepovirus, and strawberry latent ringspot nepovirus proteins in reticulocyte lysate. | the genomic rna components of three nepoviruses, arabis mosaic (armv), cherry leaf roll (clrv), and strawberry latent ringspot (slrv), were translated in rabbit reticulocyte lysate. each component (except the rna-2 of clrv) directed the synthesis of proteins that corresponded in size to their theoretical coding capacity. the rna-1 components of all three viruses were translated to yield polyproteins of mr 250k, which were autocatalytically processed to yield up to five cleavage products. the pri ... | 1991 | 1929877 |
| the complete nucleotide sequence and genome organization of lychnis mottle virus. | the complete genomic sequence of lychnis mottle virus (lycmov) from a lychnis cognata plant was determined. lycmov has a bipartite genome consisting of rna1 (7,428 nt) and rna2 (3,734 nt). species in the family secoviridae are demarcated based on their amino acid similarities in the protease-polymerase and coat protein. in lycmov, these proteins share 90% and 63% sequence similarity, respectively, with the most closely related virus, strawberry latent ringspot virus, which is a member of the fam ... | 2015 | 26259831 |
| the complete genome sequences of two isolates of cnidium vein yellowing virus, a tentative new member of the family secoviridae. | we determined the complete genome sequences of two isolates of cnidium vein yellowing virus (cnvyv-1 and -2) that co-infected all field samples collected from cnidium officinale in korea. unlike cnvyv-2, however, cnvyv-1 was sap-transmissible to nicotiana benthamiana. cnvyv-1 and -2 have bipartite genomes of 7,263 and 3,110 nucleotides and 7,278 and 3,112 nucleotides, respectively, excluding the poly(a) tails. phylogenetic analysis of the cnvyv-1 and -2 sequences indicated close relationships to ... | 2015 | 26282235 |
| multiplex nested reverse transcription-polymerase chain reaction in a single tube for sensitive and simultaneous detection of four rna viruses and pseudomonas savastanoi pv. savastanoi in olive trees. | abstract a multiplex nested reverse transcription-polymerase chain reaction (rt-pcr) in a single closed tube was developed for the simultaneous detection of four rna viruses: cucumber mosaic virus, cherry leaf roll virus, strawberry latent ringspot virus, and arabis mosaic virus, and the bacterium pseudomonas savastanoi pv. savastanoi. the method enabled, for the first time, the sensitive and simultaneous detection of rna and dna targets from plant viruses and a bacterium, saving time, decreasin ... | 2003 | 18944338 |
| novel approaches to mitigate primer interaction and eliminate inhibitors in multiplex pcr, demonstrated using an assay for detection of three strawberry viruses. | multiplex pcr is an important technique for detecting a variety of pathogens simultaneously in a single assay. previous research has focused on optimising the factors affecting reliable multiplex pcr, including primer design, pcr components and conditions, and inhibitors in samples. in this study, the interaction of primers to form complex secondary structures including visible dimers and invisible "primer clusters", a novel form of primer secondary structure found during this research, were sho ... | 2008 | 18453003 |
| a virus between families: nucleotide sequence and evolution of strawberry latent ringspot virus. | several clones of golden ginger mint (mentha x gracilis, 'variegata') were found infected with strawberry latent ringspot virus (slrsv). the virus was purified and cloned and the complete nucleotide sequence of a mint isolate was obtained. rna 1 consists of 7,496 nucleotides excluding the poly-a tail and encodes a polyprotein with signature enzymatic motifs found in other picorna-like plant viruses. rna 2 consists of 3,842 nucleotides excluding the poly-a tail, encoding a polyprotein that is pro ... | 2006 | 16837095 |
| plant parasitic and vector nematodes associated with asiatic and oriental hybrid lilies. | a survey for distribution and abundance of plant parasitic nematodes in fields grown to lilium in himachal pradesh, india at four study sites viz. nagrota (at 810 m a.s.l.), palampur (at 1270 m a.s.l.), sunder nagar (at 1400 m a.s.l.) and chail (at 2250 m a.s.l.) was carried out. moderate (101-500/200 ml soil) to high (501-1000/200 ml soil) populations of phytonematodes including the vectors for plant viruses (aphelenchoides avenae, criconemoides spp., hoplolaimus spp., longidorus spp., paratyle ... | 2006 | 15896955 |
| simultaneous and co-operational amplification (co-pcr): a new concept for detection of plant viruses. | a new and highly sensitive method for the amplification of viral rna targets from plant material has been developed and patented. this technique called co-operational amplification (co-pcr) can be carried out easily in a simple tetraprimer reaction based on the simultaneous action of four primers. the reaction process consists of the simultaneous reverse transcription of two different fragments from the same target, one containing the other; the production of four amplicons by the combination of ... | 2002 | 12367729 |