| identification of an amsacta spheroidin-like protein within the occlusion bodies of choristoneura entomopoxviruses. | like baculoviruses, a characteristic feature of entomopoxviruses is the amalgamation of virions within environmentally stable occlusion bodies. it is this occluded form of the virus that is primarily responsible for dissemination to other insects. while the major protein (polyhedrin) of baculovirus occlusions is quite similar between viruses, it has been reported that the major occlusion body protein (spheroidin) of two group b entomopoxviruses, amsacta moorei (amepv) and choristoneura biennis ( ... | 1993 | 8517016 |
| cloning, sequencing and transcriptional analysis of the choristoneura fumiferana entomopoxvirus spheroidin gene. | the choristoneura fumiferana entomopoxvirus (cfepv) spheroidin gene was identified and localized on three xbai restriction fragments (total size 4.73 kb). the fragments were cloned and sequenced. the spheroidin gene had an open reading frame of 2997 nucleotides encoding a putative protein with a predicted size of 115 kda. sequence analysis indicated that the putative protein contained 14 potential n-glycosylation sites (asn-x-thr; asn-x-ser), that are probably not used since the protein migrates ... | 1997 | 9085546 |
| characterization of the nucleoside triphosphate phosphohydrolase i gene from the choristoneura fumiferana entomopoxvirus. | poxviruses carry the enzyme, nucleoside triphosphate phosphohydrolase i (nph i), required for early viral transcription in the cytoplasm of infected cells. the gene (nph i) encoding this enzyme from choristoneura fumiferana entomopoxvirus (cfepv) has been located in the viral genome, cloned and characterized. it has an open reading frame of 1941 nucleotides, potentially encoding a protein with a predicted molecular mass of 76.04 kda and a pi of 8.83. it has a taaatg motif where the trinucleotide ... | 1998 | 9784068 |
| choristoneura fumiferana entomopoxvirus prevents metamorphosis and modulates juvenile hormone and ecdysteroid titers. | larvae of the spruce budworm, choristoneura fumiferana, infected with c. fumiferana entomopoxvirus (cfepv) continue to feed and grow without undergoing metamorphosis and die as moribund larvae. the lethal dose (ld(50)) and lethal time (lt(50)) values for fourth instar larvae are 2.4 spheroids and 25.2 days, respectively. one hundred percent of the control fourth instar larvae, which were fed water instead of virus, pupated by 18 days post feeding (pf). only 30% of the larvae that were fed the ld ... | 2000 | 10876132 |