| phylogenetic analyses of virus isolates in the genus hantavirus, family bunyaviridae. | we constructed a phylogenic tree by pairwise comparison of the deduced amino acid sequences encoded in the medium (m) genome segments of 13 hantaviruses. five distinct branches were identified, four of which are represented by hantaan, seoul, puumala, and prospect hill viruses. these groups corresponded to those previously established by serological and genetic means. in addition, we present sequence information for the m segment of thailand virus and demonstrate that it represents a unique, fif ... | 1994 | 8259656 |
| molecular characterization of a distinct begomovirus infecting tobacco in yunnan, china. | virus isolate y3v, obtained from tobacco showing leaf curl symptoms in yunnan, china, had particles with the size and morphology typical of geminiviruses. in reactions with a set of monoclonal antibodies raised against begomoviruses, y3v was readily differentiated from two previously studied chinese begomovirus isolates. the complete nucleotide sequence of a dna-a-like molecule of y3v was determined; it comprises 2744 nucleotides and has a typical begomovirus genome organization. when compared w ... | 2001 | 11676420 |
| molecular characterization of squash leaf curl yunnan virus, a new begomovirus and evidence for recombination. | virus isolate y23v, obtained from squash showing leaf curl symptoms in yunnan, china, was readily differentiated from four studied chinese begomovirus isolates in reactions with a set of monoclonal antibodies raised against begomoviruses. the complete nucleotide sequence (2714 nts) of the dna-a-like molecule of y23v was determined. the dna-a of y23v is most closely related to that of tomato yellow leaf curl thailand virus-[1] (tylcthv-[1]) (84% sequence identity). however, the ac1 and ac4 gene o ... | 2003 | 14551824 |
| molecular characterization of tomato-infecting begomoviruses in yunnan, china. | the importance of diseases of tomato caused by begomoviruses is increasing worldwide. here, we report that several begomoviruses are associated with tomato leaf curl disease in yunnan province, china. 14 tomato samples showing leaf curl symptoms were collected in three districts in yunnan, and they fell into four groups according to their reaction with a panel of 16 monoclonal antibodies in tas-elisa. comparison of partial dna-a sequences amplified with degenerate primers confirmed the existence ... | 2004 | 15593415 |
| molecular characterization of tomato-infecting begomoviruses in thailand. | bipartite geminiviruses infecting tomatoes in thailand were detected by polymerase chain reaction (pcr) using cpa5/cpa2 primers. products derived from pcr-amplified full-length dna-a and dna-b of tylcv collected from chiang mai, nong khai, and sakon nakhon were cloned and sequenced. dna-a from chiang mai was 2747 nts long; nong khai, 2744 nts; and sakon nakhon, 2747 nts, and those of dna-b from chiang mai were 2750 nts long; nong khai, 2749 nts; and sakon nakhon, 2749 nts. the genomes of these v ... | 2004 | 15826907 |
| detection of tomato yellow leaf curl thailand virus by pcr without dna extraction. | we report the simple and rapid method for detection of tomato yellow leaf curl thailand virus (tylcthv) based on the direct capture of virus particles to the surface of a polymerase chain reaction (pcr) tube. this method allowed pcr without the time-consuming procedures of dna extraction from infected plant tissue. a small amount of tomato tissue (approximately 10 mg) was ground in extraction buffer to release viruses from plant tissues. the constituents of the plant extract that might inhibit p ... | 2005 | 16230773 |
| genetic analysis of thailand hantavirus in bandicota indica trapped in thailand. | sixty one tissue samples from several rodent species trapped in five provinces of thailand were examined for the presence of hantaviral markers by enzyme-immunoassay and immunoblotting. four samples, all from the great bandicoot rat bandicota indica, were confirmed positive for the hantaviral n-antigen. two of them were trapped in nakhon pathom province, the other two in nakhon ratchasima province, approximately 250 km from the other trapping site. when analysed by rt-nested pcr, all four rodent ... | 2006 | 16953877 |
| geographical distribution of hantaviruses in thailand and potential human health significance of thailand virus. | phylogenetic investigations, sequence comparisons, and antigenic cross-reactivity studies confirmed the classification of thailand virus (thaiv) as a distinct hantavirus species. the examination of sera from 402 rodents trapped in 19 provinces of thailand revealed that five greater bandicoot rats (bandicota indica) and one lesser bandicoot rat (b. savilei) from four provinces were focus reduction neutralization test (frnt) antibody-positive for thaiv. one of 260 patients from surin province in t ... | 2006 | 17124002 |
| application of phi29 dna polymerase in identification and full-length clone inoculation of tomato yellow leaf curl thailand virus and tobacco leaf curl thailand virus. | tomato plants grown in greenhouses in thailand developed typical symptoms of a tomato yellow leaf curl thailand virus (tylcthv) infection. after confirmation by elisa, a phi29 dna polymerase approach was chosen for further molecular analysis of tylcthv. total dna purified from infected tomato leaves was subjected to rolling-circle amplification (rca) of dna-a and dna-b of tylcvthv. in addition, a new monopartite geminivirus with a putative recombinant background was identified by rca and tentati ... | 2007 | 17226067 |
| transreplication of a tomato yellow leaf curl thailand virus dna-b and replication of a dnabeta component by tomato leaf curl vietnam virus and tomato yellow leaf curl vietnam virus. | the genomes of two tomato-infecting begomoviruses from vietnam were cloned and sequenced. a new variant of tomato leaf curl vietnam virus (tolcvv) consisting of a dna-a component and associated with a dnabeta molecule as well as an additional begomovirus tentatively named tomato yellow leaf curl vietnam virus (tylcvv) consisting also of a dna-a component were identified. to verify if monopartite viruses occurring in vietnam and thailand are able to transreplicate the dna-b component of tomato ye ... | 2008 | 18550192 |
| development of a serotyping elisa system for thailand virus infection. | to distinguish thailand virus infection from infections with other hantaviruses, we established an elisa serotyping system using a truncated nucleocapsid protein of thailand virus lacking 49 amino acids at the n-terminus. in evaluations using patient and rodent sera, thailand virus infection was readily distinguished from hantaan and seoul virus infections. therefore, this elisa system is an effective alternative to neutralization tests. | 2008 | 18551243 |
| a newly recognized hantavirus in the asian house rat (rattus tanezumi) in indonesia. | hantaviral sequences were recovered from the lung tissue of an asian house rat (rattus tanezumi) captured in serang, indonesia. phylogenetic analysis of partial l, m and s segment sequences showed that they belonged to a novel hantavirus provisionally named serang virus (serv). notably, serv is distinct from the hantaviruses associated with rodents of the species rattus: seoul virus associated with rattus norvegicus worldwide and gou virus isolated from rattus rattus in china. instead serv appea ... | 2009 | 19088290 |
| recent discoveries of new hantaviruses widen their range and question their origins. | hantaviruses belong to the bunyaviridae family. while usually hosted by wild mammals, they are potentially pathogenic for humans, and several serologically distinct groups associated with different syndromes have been identified. yet, investigations have mostly been conducted where human infections by hantaviruses constitute a real and well-identified public health problem, i.e., the holarctic and neotropical areas. some hantaviruses have also been described from a suncus murinus in india and a ... | 2008 | 19120180 |
| tomato yellow leaf curl thailand virus-[y72] from yunnan is a monopartite begomovirus associated with dnabeta. | previous studies have shown that isolates of tomato yellow leaf curl thailand virus (tylcthv) originated from thailand are bipartite begomoviruses, while all the seven tylcthv isolates found in china are associated with dnabeta molecules. in this study, infectious clones of tylcthv isolate y72 (tylcthv-[y72]) and its dnabeta were constructed to verify the bipartite or monopartite nature of tylcthv. agroinoculation showed that tylcthv-[y72] alone was able to induce significant symptoms in nicotia ... | 2009 | 19165588 |
| hantavirus species in india: a retrospective study. | hantaviruses cause hemorrhagic fever with renal syndrome in europe and asia. there are about 20 documented hantavirus species and newer species are being described worldwide, especially in non-rodent reservoirs, i.e shrews. focus reduction neutralization test is the classical serotyping technique for hantavirus. however, this study employs a previously established serotyping elisa, to retrospectively analyze known hantavirus igg reactive samples for infecting serotypes. the result suggests prese ... | 2009 | 19736405 |
| molecular characterization of two hantavirus strains from different rattus species in singapore. | hantaviruses cause human disease in endemic regions around the world. outbreaks of hantaviral diseases have been associated with changes in rodent population density and adaptation to human settlements leading to their proliferation in close proximity to human dwellings. in a parallel study initiated to determine the prevalence of pathogens in singapore's wild rodent population, 1206 rodents were trapped and screened. the findings established a hantavirus seroprevalence of 34%. this paper descri ... | 2010 | 20096099 |
| tomato yellow leaf curl viruses: ménage à trois between the virus complex, the plant and the whitefly vector. | tomato yellow leaf curl disease (tylcd) is one of the most devastating viral diseases affecting tomato crops in tropical, subtropical and temperate regions of the world. here, we focus on the interactions through recombination between the different begomovirus species causing tylcd, provide an overview of the interactions with the cellular genes involved in viral replication, and highlight recent progress on the relationships between these viruses and their vector, the whitefly bemisia tabaci. t ... | 2010 | 20618703 |
| serological evidence of thailand virus-related hantavirus infection among suspected leptospirosis patients in kandy, sri lanka. | a cross-sectional study was undertaken to determine the current prevalence of leptospirosis and hantaviral infections, and the socio-demographic characteristics and risk factors of infected patients, in kandy, sri lanka. this report discusses the serological evidence of hantavirus infections among 105 suspected leptospirosis patients, 8 of whom had hantavirus antibodies. serotyping elisa showed that these 8 patients had high optical density values for thailand virus. most of the sera showed that ... | 2011 | 21266762 |
| an optimized mrfp-based bimolecular fluorescence complementation system for the detection of protein-protein interactions in planta. | an existing bimolecular fluorescence complementation (bifc) system, based on a monomeric red fluorescent protein (mrfp), has been optimized for the investigation of protein-protein interactions in planta. the expression plasmids, encoding the n-terminal amino acids (aa) 1-168 and the c-terminal aa 169-225 of the mrfp, allow n- or c-terminal fusion of a split mrfp, with the genes of interest. two major improvements over the original vectors have been made. firstly, the coding sequence of a gggsgg ... | 2011 | 21473882 |
| Rodent-Borne Hantaviruses in Cambodia, Lao PDR, and Thailand. | In order to evaluate the circulation of hantaviruses present in southeast Asia, a large scale survey of small mammal species was carried out at seven main sites in the region (Cambodia, Lao People's Democratic Republic, and Thailand). Small scale opportunistic trapping was also performed at an eighth site (Cambodia). Using a standard IFA test, IgG antibodies reacting to Hantaan virus antigens were detected at six sites. Antibody prevalence at each site varied from 0 to 5.6% with antibodies detec ... | 2011 | 22124701 |
| application of truncated nucleocapsid protein (n) for serotyping elisa of murinae-associated hantavirus infection in rats. | truncated recombinant nucleocapsid proteins (trns) that lack n-terminally located cross-reactive epitopes of four murinae rodent-associated hantaviruses, seoul virus (seov), thailand virus, hantaan virus (htnv) and dobrava-berglade virus, were produced by using a baculovirus expression system. elisa with the trns as antigens enabled serotyping of immune sera from rats experimentally inoculated with the corresponding hantaviruses as cut-off od values of 60% to those to whole n of htnv. the trn ba ... | 2011 | 21937858 |
| hantavirus infection among wild small mammals in vellore, south india. | wild indigenous small mammals including 83 rodents (bandicoot and black rats, and house mice) and a shrew captured from multiple sites in vellore, south india, were tested for serological and molecular evidence of hantavirus infection. indirect immunofluorescence assay (ifa) using hantaan virus (htnv) antigen indicated hantavirus-reactive antibodies in 16 (19.3%) of 83 rodents (bandicoot and black rats). western blot (wb) using thailand virus (thaiv) antigen confirmed hantavirus-reactive antibod ... | 2013 | 22856552 |
| discovery of hantavirus circulating among rattus rattus in french mayotte island, indian ocean. | hantaviruses are emerging zoonotic viruses that cause human diseases. in this study, sera from 642 mammals from la réunion and mayotte islands (indian ocean) were screened for the presence of hantaviruses by molecular analysis. none of the mammals from la réunion island was positive, but hantavirus genomic rna was discovered in 29/160 (18 %) rattus rattus from mayotte island. the nucleoprotein coding region was sequenced from the liver and spleen of all positive individuals allowing epidemiologi ... | 2016 | 26932442 |
| anjozorobe hantavirus, a new genetic variant of thailand virus detected in rodents from madagascar. | until now, there was only serological evidence that hantaviruses were circulating in rodents and infecting humans from madagascar. to assess the presence of a hantavirus on the island, between october, 2008, and march, 2010, we sampled 585 rodents belonging to seven species in the anjozorobe-angavo forest corridor, 70 km north from the capital city antananarivo. a hantavirus was detected from organs of the ubiquist roof rat (rattus rattus) and of the endemic major's tufted-tailed rat (eliurus ma ... | 2014 | 24575755 |
| emergence and diversity of begomoviruses infecting solanaceous crops in east and southeast asia. | over the past three decades diseases caused by whitefly-transmitted geminiviruses (begomoviruses) have emerged to be important constraints to the production of solanaceous crops, particularly tomato (solanum lycopersicum) and peppers (capsicum spp.), in many tropical and subtropical regions of the world. the most studied of these is tomato yellow leaf curl virus (tylcv), which has spread to many other areas from its likely origin in the mediterranean basin region. the virus is usually associated ... | 2014 | 24440320 |
| development and application of triple antibody sandwich enzyme-linked immunosorbent assays for begomovirus detection using monoclonal antibodies against tomato yellow leaf curl thailand virus. | tomato yellow leaf curl thailand virus, tylcthv, is a begomovirus that causes severe losses of tomato crops in thailand as well as several countries in southeast and east asia. the development of monoclonal antibodies (mabs) and serological methods for detecting tylcthv is essential for epidemiological studies and screening for virus-resistant cultivars. | 2017 | 28558726 |