| virion heat-sensitivity of adenovirus type 5 temperature-sensitive mutants and interferon induction. | kinetics of interferon induction with wild type adenovirus 5, and three temperature-sensitive mutants, ts 1, ts 18 and ts 19 were examined in chick embryo cells (cec) at permissive (31 degrees c) and non-permissive (38 degrees c) temperatures. interferon levels reached a maximum four days after infection with the wild type and all ts mutants at 31 degrees c. however, at 38 degrees c, ts 18 and ts 19 failed to induce interferon, while wild type and ts 1 induced normal levels which reached a maxim ... | 1976 | 9819 |
| complex formation between the adenovirus type 5 dna-binding protein and single-stranded dna. | | 1978 | 26566 |
| infectivity titers of adenovirus type 5 suspensions after exposure to cigarette smoke. | suspension of adenovirus type 5 in 2.0 ml of cell culture fluid at 37 degrees c were subjected to smoke from four cigarettes over a 4-h period. the cigarettes were smoked in a normal manner, and the inhaled smoke was exhaled through glass tubing into the virus-containing fluid. the virus suspensions were then titrated, using monolayer cultures of hep-2 cells. smoke from filter-tipped or regular cigarettes caused a 2- to 3-log drop in titer of tissue culture infectious doses of adenovirus type 5 ... | 1978 | 31840 |
| structural elements in adenovirus cores. evidence for a "core shell" and linear structures in "relaxed" cores. | "freeze-fracture negative staining" of adenovirus type 5 revealed the virus cores as internal bodies with a fine granular surface which at high magnification shows reticular and ring-like patterns. this indicates that the virus protein v forms a thin surface layer for which a name "core shell" is proposed. virus cores prepared by heating virus particles in sodium deoxycholate (doc) relaxed into curved filaments or several rods by means of egta and high salt, respectively. high ph treatment had s ... | 1979 | 44463 |
| a negative staining-carbon film technique for studying viruses in the electron microscope. ii. application to adenovirus type 5. | | 1975 | 47916 |
| fine structure of adenovirus type 5. i. virus capsid. | | 1975 | 48307 |
| radioimmunoassay of human serum antibody specific for adenovirus type 5-purified fiber. | a radioimmunoassay (ria), utilizing a second antibody to separate immune complexes, was developed to provide a sensitive and specific measure of serum antibody to adenovirus type 5 (ad 5) fiber. purity of fiber antigen was ascertained by sodium dodecyl sulfate urea-polyacrylamide gel electrophoresis and isoelectric focusing in ampholyte ph gradients. after labeling with 125i to high specific activity, the iodinated fiber did not exhibit loss of antigenic reactivity and remained stable for 3 week ... | 1975 | 50343 |
| a study of possible biohazards in the fluorescent antibody test using adenovirus, coxsackievirus, herpesvirus, and respiratory syncytial virus as antigens. | infectious adenovirus type 5 and coxsackievirus type b5, both nonlipid-containing viruses, were isolated from cells fixed in acetone at 22 degrees c for 15 min, from acetone used for fixation, from the solution used for washing slides during the fluorescent antibody procedure, and after complete processing of antigen preparations with serial twofold dilutions of human antisera and fluorescein-labeled goat anti-human immunoglobulin g. lipid-containing herpes simplex virus type 1 and respiratory s ... | 1976 | 61971 |
| measurement of human type-specific antibody to adenovirus type 5 hexon and fiber using a fab'-blocked antigen probe. | | 1979 | 87446 |
| detection of adenovirus by enzyme-linked immunosorbent assay. | a solid-phase direct enzyme-linked immunosorbent assay (elisa) was developed for the detection of adenovirus antigen in extracts of infected cells by using antihexon serum. results with simulated clinical specimens consisting of normal nasal wash specimens seeded with varying concentrations of adenovirus type 5 showed that antigen could be detected in extracts of hep-2 cell cultures inoculated with 10(2.5) 50% tissue culture infective doses (tcid50) and 10(1.5) tcid50 after 2 and 4 days of incub ... | 1979 | 88457 |
| [decrease in stimulation and allogeneic response following experimental infection of mice with human adenovirus 5]. | 15 days after experimental infection with human adenovirus 5, c57 bl/6 murine spleen cells were found to have altered in vitro properties. proliferative response to phytohemagglutinine was slightly increased while the allogeneic response was decreased and the capability to stimulate allogeneic cells was significantly diminished. the degree of expression of surface antigens responsible for allogeneic stimulation is influenced by many regulatory factors, still poorly defined; alteration of some fa ... | 1978 | 103640 |
| differential cell membrane labilizing effect of adenovirus type 5 and 12 observed during productive and abortive infection. | culture fluid of human epitheloid (hep-2) cells was examined for extracellular lactate dehydrogenase activity as an indicator of cell damage during a 48 h period in which virus replication and changes in cell morphology occurred. uninfected and adenovirus type 5-infected cells had the same levels of extracellular enzyme activity both before and after the appearance of morphological changes in cells due to virus infection, whereas adenovirus type 12-infected cells showed increased extracellular e ... | 1979 | 119136 |
| initiation of adenovirus dna replication does not occur via a hairpin mechanism. | models have been proposed suggesting that initiation of adenovirus dna replication might occur via a hairpin mechanism. a consequence of the models is a covalent linkage of progeny and parental dna in newly completed molecules. analysis of mature molecules from kb cells infected with adenovirus type 5 indicates that, if a hairpin mechanism is involved, the length of the hairpin must be shorter than 50 basepairs long. recent nucleotide sequence analysis of the termini of adenovirus type 5 dna (p. ... | 1978 | 148639 |
| lesions and pathogenesis of disease in young calves experimentally induced by a bovine adenovirus type 5 isolated from a calf with weak calf syndrome. | lesions induced in each of 9 young colostrum-deprived calves closely resembled lesions seen in naturally occurring "weak calf syndrome" of eastern idaho and southwestern montana. the disease was experimentally induced by intravenous injection of bovine adenovirus type 5 that had been isolated from a calf with weak calf syndrome... | 1975 | 168796 |
| properties and behavior of hamster embryo cells transformed by human adenovirus type 5. | | 1975 | 169093 |
| viral dna sequences in cells transformed by simian virus 40, adenovirus type 2 and adenovirus type 5. | | 1975 | 169095 |
| acute posterior multifocal placoid pigment epitheliopathy associated with an adenovirus type 5 infection. | a 19-year-old black man with acute posterior multifocal placoid pigment epitheliopathy had concurrent positive viral cultures and rising antibodies to adenovirus type 5. this finding, considered together with earlier reports of viral-like syndromes accompanying the disease, supports the implication of a respiratory virus as a possible etiologic factor. | 1975 | 173190 |
| studies of the uv-sensitivity of virus-specific rna synthesis in cells infected or transformed by adenovirus 5 and sv 40. | the effect of uv-irradiation on host and virus-specific rna synthesis in cells infected or transformed by tumor viruses (ad 5 and sv 40) was studied. it was found that the synthesis of host and ad 5 rna in infected kb cells was almost euqally inhibited upon uv-irradiation; the transcription of the ecori produced fragements--fragment b was inhibited to a greater extent than fragment a, suggesting that the transcription of the whole ad 5 genome starts from the left side. the transcription of viral ... | 1977 | 192999 |
| purified dnas are transcribed after microinjection into xenopus oocytes. | the possibility of using dna-injected xenopus laevis oocytes and eggs for studying the control of transcription in eukaryotes has been investigated. when purified dna of simian virus 40 (sv40) is injected into xenopus laevis oocytes, tritiated rna precursors are incorporated into dnase-i-resistant, rnase-a- and alkali-sensitive material that hybridizes specifically to sv40 dna. this viral transcription continues for at least 5 days and occurs only when the injected dna is directed to the nucleus ... | 1977 | 193103 |
| virions associated with acute gastroenteritis in vancouver, 1976. | virions were identified by electron microscopic observation of unconcentrated extracts of feces obtained from 55 (33%) of 167 children aged 3 weeks to 5 years who were hospitalized with acute gastroenteritis at children's hospital, vancouver between january and december 1976. morphologic types included 30 rotavirus, 15 adenovirus, 5 astrovirus and 5 picornavirus-like particles. | 1977 | 199339 |
| ribosomal proteins in normal simian cells, sv40-transformed simian cells, and simian cells infected with sv40, adenovirus 5, and vesicular stomatitis virus. | the protein patterns of monosomes and polysomes isolated from the t-22 line of sv40-transformed gmk cells and from uninfected cv-1 cells and cv-1 cells infected with sv40, adenovirus 5, or vesicular stomatitis virus were analyzed by two-dimensional page. all gel patterns were similar except for the presence of one additional protein associated with t-22 monosomes. | 1978 | 202574 |
| some adenovirus dna is associated with the dna of permissive cells during productive or restricted growth. | we have investigated the association of viral dna with cell dna in chicken embryo kidney (cek) cells productively infected with chicken embryo lethal orphan (celo) virus and in human (hek) cells infected with mutants ts36 and ts125 of human adenovirus type 5 under permissive and restrictive conditions. cell and viral dna molecules were separated after celo virus infection of cek cells by alkaline sucrose gradient centrifugation, network formation, and cscl density gradient centrifugation, method ... | 1978 | 202728 |
| possible role of the 72,000 dalton dna-binding protein in regulation of adenovirus type 5 early gene expression. | relative abundances of early virus rna species in the cytoplasm of cells infected with wild-type adenovirus type 5 (wt ad5) and a temperature-sensitive "early" mutant, h5ts125 (ts125), were compared by hybridization kinetics using separated strands of hindiii restriction endonuclease fragments of ad5 dna. 1-beta-d-arabinofuranosylcytosine (ara-c) was used to limit transcription to early virus genes in cells infected by wt virus. at 40.5 degrees c, a restrictive temperature for ts125, three to se ... | 1978 | 203722 |
| enhanced infectivity of adenovirus type 2 dna and a dna-protein complex. | the infectivity of adenovirus type 2 dna and a dna-protein complex was studied in 293 cells, a human embryonic kidney cell line transformed by sheared adenovirus type 5 dna, and in human kb cells. adenovirus type 2 dna was more infectious (up to about 40-fold) in 293 cells than in kb cells, whereas a dna-protein complex (prepared by a rapid procedure) had about the same infectivity in both cell lines. these data may mean that a factor present in 293 cells (perhaps a viral-coded protein) enhances ... | 1978 | 206726 |
| adenovirus-induced inhibition of cellular dnase. | during the productive infection of kb cells by adenovirus type 5 (ad5), there is a progressive decrease in the level of cellular dnase activity towards single-stranded dna, in contrast to dna polymerase which remains relatively constant throughout the infection. this decrease is prevented by the inhibition of protein synthesis by cycloheximide. the inhibition of dnase activity does not occur after infection by ad5 ts125, a dna-negative mutant which fails to induce the adenovirus-specific dna bin ... | 1978 | 207901 |
| infection of mouse liver by human adenovirus type 5. | cba mice, inoculated intravenously with large doses of adenovirus type 5, showed raised levels of serum aspartate aminotransferase (saat; ec 2.6.i.i) and died within a few days from histologically demonstrable hepatic necrosis. after inoculation of i ld50, virus was rapidly taken up by the tissues where infectivity then declined greatly. organ titres then increased about 100-fold by 48 h p.i. but, in the liver, which showed intranuclear inclusion bodies, and by electron microscopy, scattered int ... | 1978 | 211182 |
| herpesvirus infection modifies adenovirus rna metabolism in adenovirus type 5-transformed cells. | the effect of herpes simplex virus (hsv) infection of mrna metabolism was examined in a system where the fate of specific rna sequence can be assayed. adenovirus type 5-transformed rat embryo cell line 107 synthesizes adenovirus-specific rna (ad-rna), which functions in the cytoplasm as mrna. we have utilized ad-rna as a model for mrna metabolism, and in a preliminiary study we characterized ad-rna in the nucleus and cytoplasm by hybridization to filter-bound adenovirus dna. the results indicate ... | 1978 | 211246 |
| interactions of shope papilloma virus with some other dna viruses. | the interactions of shope papilloma virus (spv) with primate and rabbit cells in tissue culture have been investigated. the rabbit cell cultures were derived from normal epidermis, from spv-infected epidermis, from spv-induced papillomas, and from an spv-associated carcinoma. none of these cell cultures, whether infected in vitro with spv or derived from tissues infected in vivo, ever produced infectious spv or even detectable viral antigens. some other dna viruses behaved differently in cells w ... | 1978 | 212147 |
| autoregulation of adenovirus type 5 early gene expression ii. effect of temperature-sensitive early mutations on virus rna accumulation. | the kinetics of accumulation of early virus rna in the cytoplasm of kb cells infected at 40.5 degrees c by wild-type (wt) adenovirus type 5 and a temperature-sensitive "early" mutant, h5ts125 (ts125), were compared by hybridization of unlabeled rna in solution to the (3)h-labeled l strand of ad5 dna hindiii restriction endonuclease fragment a. in the presence of 1-beta-d-arabinofuranosylcytosine, a(l) rna accumulated in wt-infected cells for 9 h and then decreased in concentration to 6% of the 9 ... | 1978 | 214573 |
| the effect of mixed infections induced by adenovirus type 5 and vaccinia or smallpox virus, on the ultrastructure of kb cells ii. observations of changes in the ultrastructure of kb cells during 6--11 days infection with adenovirus type 5 and vaccinia virus. | | 1978 | 215254 |
| the effect of mixed infections induced by adenovirus type 5 and vaccinia or smallpox virus, on the ultrastructure of kb cells. iii. changes in the ultrastructure of kb cells within 6--11 days infection with adenovirus type 5 and smallpox virus. | | 1978 | 215255 |
| adeno-associated virus dna replication complexes in herpes simplex virus or adenovirus-infected cells. | a complex which is active in in vitro synthesis of adeno-associated virus (aav) dna was solubilized from vero cells that were co-infected with aav and either adenovirus (ad5) or a herpes simplex virus type 1 (hsv-1) as the helper virus. the complexes from the ad5 and hsv-1-infected cells sedimented at 23 s and 28 s, respectively. the optimal conditions for in vitro dna synthesis for the two types of complex using the endogenous aav template and the endogenous dna polymerase, differed with respec ... | 1979 | 221504 |
| sequence of inverted terminal repetitions from different adenoviruses: demonstration of conserved sequences and homology between sa7 termini and sv40 dna. | we have established the nucleotide sequence for the inverted terminal repetition of human adenovirus type 3, a subgroup b adenovirus. the repetition, which is 136 bp long, shows a high degree of homology with the known sequence for the inverted repetition of adenovirus type 5 (steenbergh et al., 1977) a subgroup c adenovirus. partial sequence information convering 120 bp of the inverted terminal repetitions of human serotype 12, a subgroup a member, and of simian adenovirus type 7 has also been ... | 1979 | 225041 |
| effects of viral exposure of the two-cell mouse embryo on cleavage and blastocyst formation in vitro. | the effect of viral exposure of two-cell mouse embryos on their capacity to undergo subsequent cleavage and blastocyst formation in vitro was determined. exposure to coxsackie viruses b-4 and b-6, reovirus type 2, influenza virus type a, mouse cytomegalovirus, adenovirus type 5, and mouse adenovirus resulted in statistically significant inhibition of blastocyst formation. development in vitro was unaffected by exposure to echo virus type 11, attenuated poliomyelitis virus type 2, parainfluenza v ... | 1979 | 225719 |
| interaction of human lymphocytes and viruses in vitro. | interaction between phytohaemagglutinin (pha) stimulated human lymphocytes and two dna viruses (adenovirus type 5 and herpes simplex virus type 1) considered to be closely connected with lymphoid tissues has been studied. the fate of the same viruses was investigated also in non-stimulated separated lymphocytes for comparative purposes. to elicit this interaction infectivity titrations, immunofluorescent technique and electron microscopy were used. the production of viral antigens was investigat ... | 1979 | 225930 |
| further studies of antibody levels to herpes simplex virus, cytomegalovirus, measles virus, and adenovirus type 5 in burkitt's lymphoma patients. | sera collected from burkitt's lymphoma (bl) patients before and after tumor manifestation were tested for antibodies to herpes simplex virus (hsv), cytomegalovirus (cmv), measles virus (mv) and adenovirus type 5, using the immunofluorescent (if) techniques in all instances except for mv where complement fixation (cf) was used. it was found that none of these viruses showed higher antibody levels in bl patients than in controls, either before or after the appearance of the tumor. the patients cam ... | 1979 | 226855 |
| presence of protein at the termini of intracellular adenovirus type 5 dna. | adenovirus type 5 contains linear double-stranded dna with protein covalently attached to the ends of the molecules. the presence of protein at the termini of intracellular viral dna in adenovirus type 5-infected cells was investigated at different stages during the replication process. the intracellular viral dna was isolated from the nuclei by lysis in 4 m guanidine hydrochloride. electrophoresis on agarose gels of hsui restriction enzyme fragments and sucrose gradient centrifugation were used ... | 1979 | 227463 |
| characterization of virus dna synthesized in kb cells infected with two temperature-sensitive mutants of adenovirus type 5. | kb cells were infected with h5ts36 or h5ts125, two adenovirus type 5 (ad5) mutants with a temperature-sensitive synthesis of virus dna. infection was started at the nonpermissive temperature and at 16 h p.i. the temperature was shifted down to the permissive temperature. shortly after the shift-down h5ts125-infected cells showed an accumulation of purely single-stranded dna of virus origin, which was not observed in h5ts36-infected cells. this single-stranded dna has been characterized by hybrid ... | 1979 | 227996 |
| modulation of herpes simplex virus replication in adenovirus transformed cells. | the ability of herpes simplex virus 1 to replicate in cells transformed by adenovirus type 5 is strongly dependent on the origin of the cells. studies show that adenovirus transformed rat cells lose their permissiveness while cells of hamster or human origin retain their ability to replicate hsv although at a reduced level when compared to the untransformed parent cells. one line of adenovirus transformed rat cells, 107, demonstrates thermosensitive events, allowing hsv to replicate at 34 degree ... | 1979 | 230284 |
| immunoprecipitation of protein kinase activity from adenovirus 5-infected cells using antiserum directed against tumour antigens. | | 1979 | 233116 |
| human adenovirus antibody in sera of normal and tumour-bearing dogs. | serum specimens from 42 normal dogs and 42 with untreated malignant tumours were assayed for the presence of antibodies to human adenovirus types 5, 21 and 31 and to infectious canine hepatitis (ich) virus. radioimmunoassays using human adenovirus antigens showed that 71 per cent (30/42) of all dogs with tumours, but only 19 per cent (8/42) of all normal dogs, were positive for human adenovirus antibody. canine sera reactive with antigens of one human adenovirus type in radioimmunoassays were al ... | 1979 | 233277 |
| the nucleotide sequence of the right-hand terminus of adenovirus type 5 dna: implications for the mechanism of dna replication. | the nucleotide sequence of the right-hand terminal 3% of adenovirus type 5 (ad5) dna has been determined, using the chemical degradation technique developed by maxam and gilbert (1977). this region of the genome comprises the 1003 basepair long hindiii-i fragment and the first 75 nucleotides of the adjacent hindiii-f fragment, extending from the right-hand terminus to the sequences from which the main body of the mrna of early region 4 is transcribed. one of the origins of adenovirus dna replica ... | 1979 | 264242 |
| interactions between adenovirus, a tumor promoter, and chemical carcinogens in transformation of rat embryo cell cultures. | the tumor-promoting agent 12-o-tetradecanoyl-phorbol-13-acetate (tpa) caused a 2- to 3-fold enhancement of transformation of secondary rat embryo cells that had been injected with a temperature-sensitive mutant of adenovirus type 5(h5ts 125). in addition, transformed foci appeared earlier and were larger in cultures grown in the presence of tpa. the addition of tpa could be delayed until up to 7 days after viral injection and still enhancement was observed. exposure of the cells to 7,12-dimethyl ... | 1978 | 276873 |
| adenovirus dna replication in vitro. | a soluble extract from the nuclei of hela cells infected with adenovirus 5 (ad5) carries out the semiconservative replication of exogenously added ad5 dna in vitro. maximal dna synthesis is observed when dna-protein complex, isolated from ad5 virions, is added as template. dna-protein complex from virions of the closely related virus, adenovirus 2, is also active. in contrast, very little in vitro dna synthesis is observed when deproteinized ad5 dna or dna from a heterologous source (bacteriopha ... | 1979 | 284391 |
| an adenovirus type 5 early gene function regulates expression of other early viral genes. | we have identified an adenovirus type 5(ad5) early gene function located in early region 1 which is required for the production of early cytoplasmic mrnas corresponding to early regions 2, 3, and 4. mutant dl312 (lacks the segment between 1.5 and 4.5 map units) grows as well as wild-type virus in 293 cells (ad5-transformed human embryonic kidney cells), but its growth is severely restricted in hela cells. we detect no viral rnas in the cytoplasm of dl312-infected hela cells. viral rna sequences ... | 1979 | 291030 |
| adenovirus dna-binding protein in cells infected with wild-type 5 adenovirus and two dna-minus, temperature-sensitive mutants, h5ts125 and h5ts149. | studies have been done to characterize further h5ts125, an adenovirus type 5 conditionally lethal, temperature-sensitive (ts) mutant defective in initiation of dna synthesis and to investigate whether the single-strand-specific dna-binding (72,000 molecular weight) protein is coded by the mutated viral gene. when h5ts125-infected cells were labeled with [35s]methionine at 32 degrees c and then incubated without isotope at 39.5 degrees c, the mutant's nonpermissive temperature, the 72,000 molecul ... | 1977 | 328925 |
| in vivo and in vitro phosphorylation of the adenovirus type 5 single strand-specific dna-binding protein. | | 1977 | 405795 |
| autoregulation of adenovirus type 5 early gene expression. iii. transcription studies in isolated nuclei. | the rate of adenovirus rna synthesis was compared in nuclei isolated from cells infected at 40.5 degrees c in the presence of 1-beta-d-arabinofuranosylcytosine with adenovirus 5 or an early temperature-sensitive mutant of adenovirus type 5, h5ts125 (ts125). in nuclei isolated at various times after infection, the maximum amount of virus rna synthesis occurred at 6 h after infection, after which time virus rna synthesis declined in nuclei from wild-type infections but remained high in nuclei from ... | 1979 | 430604 |
| human brain tumour cell strains with deficient host-cell reactivation of n-methyl-n'-nitro-n-nitrosoguanidine-damaged adenovirus 5. | | 1979 | 450131 |
| adenovirus 5 dna sequences present and rna sequences transcribed in transformed human embryo kidney cells (hek-ad-5 or 293). | | 1979 | 452423 |
| differential translation in normal and adenovirus type 5-infected human cells and cell-free systems. | when uninfected or adenovirus 5-infected kb cells are exposed to hypertonic medium, the incorporation of radioactive amino acids into protein decreases in both, but more severely in the uninfected cells. although the effect of hypertonic medium on the synthesis of specific polypeptides varies, the translation of viral polypeptides as a class is less inhibited. the same patterns of proteins are synthesized regardless of the solute used in the hypertonic medium. the mechanism by which hypertonic c ... | 1979 | 469992 |
| isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells. | a series of adenovirus type 5 (ad5) deletion, insertion and substitution mutants, some of which are defective for transformation of rat cells, have been isolated. the mutants were selected as variants which lack the xba i endonuclease cleavage site at 4 map units on the viral chromosome. the deletions range in size from 150-2300 bp and are located between 1.5 and 10.5 map units. the mutants can be propagated in 293 cells (ad5-transformed human embryonic kidney cells), but are defective for growt ... | 1979 | 476833 |
| the nucleotide sequence of the transforming hindiii-g fragment of adenovirus type 5 dna. the region between map positions 4.5 (hpai site) and 8.0 (hindiii site). | the nucleotide sequence of the region between map positions 4.5 (hpai-site) and 8.0 (hindiii-site) of adenovirus type 5 (ad5) dna has been determined. this stretch of dna is part of the transforming hindiii-g fragment, which is 2809 nucleotides long. the sequenced segment was found to have a long open reading frame for protein biosynthesis, starting 23 nucleotides from the hpai site and extending all the way to the hindiii-g site, which could code for a protein of at least 44 000 daltons. the po ... | 1979 | 478299 |
| characterization of two temperature-sensitive mutants of adenovirus type 5. | the properties of two temperature-sensitive mutants ts 18 and ts 19 of adenovirus type 5 were studied. it was demonstrated that they had a defect such that they failed to assemble virus and showed defective processing of infected cell polypeptides at the restrictive temperature. analysis, after protease digestion, of the virions produced at the permissive temperature by sds page, and of the substrate availability of the mutants to the virus protein kinase suggested that polypeptide vi was defect ... | 1979 | 479848 |
| pre-early adenovirus 5 gene product regulates synthesis of early viral messenger rnas. | | 1979 | 487437 |
| messenger ribonucleoprotein complexes in human kb cells infected with adenovirus type 5 contain tightly bound viral-coded '100k' proteins. | late after infection of kb cells with adenovirus 5 an extra protein becomes associated with messenger ribonucleoprotein particles present in the polysomes. this protein has a molecular weight of 100000 and is identical to the virus coded '100k' protein found previously. the extra protein is firmly attached to the messenger ribonucleoprotein complexes. its binding resists exposure to high salt concentrations as used in puromycin/high-salt dissociation and equilibrium centrifugation in cs2so4 grad ... | 1979 | 488096 |
| liposomal enhancement of the immunogenicity of adenovirus type 5 hexon and fiber vaccines. | immunogenicity of adenovirus capsid proteins carried in liposomes was comparable to that with equivalent doses administered in freund adjuvant, and both forms were more potent than aqueous vaccines. | 1979 | 489132 |
| the existence of dna sequences homologous to adenovirus 5 dna in the genome of normal rat cells. | three discrete bands specifically hybridizing to adenovirus 5 dna were found in the rat liver dna restricted by bam hi endonuclease and fractionated electrophoretically. the hybridization with different regions of the viral genome takes place. similar bands are present in the dna from different lines of adenovirus 5 transformed cells, but in these cases high molecular weight dna fragments containing the integrated viral genomes can also be found. | 1979 | 503857 |
| host-cell reactivation of uv-irradiated adenovirus in cockayne syndrome. | measurements of the host-cell reactivation (hcr) of mutagen-treated virus provides a very sensitive tool for detecting abnormal dna repair. the best example of the utility of hcr studies in the examination of the dna-repair capacity of human cells has come from studies of cells from the uv-sensitive repair-deficient xeroderma pigmentosum (xp) patients. we have examined the hcr of uv-treated adenovirus type 5 (ad5) and type 2 (ad2) in cells from patients with cocayne syndrome (cs), another sun-se ... | 1979 | 514294 |
| characterization of tumor antigens in cells transformed by fragments of adenovirus type 5 dna. | | 1979 | 516452 |
| spontaneous, mutagen-induced and adenovirus-induced anchorage independent tumorigenic variants of mouse cells. | normal c57 black mouse embryo cells did not form colonies in agarose, but rare variant (ar+) cells able to grow in agarose were detected. fluctuation analysis showed that ar+ variants arose by spontaneous mutation in the cultured cells. the frequency of ar+ variants was increased by treating cells with n-methyl-n'nitro-n-nitrosoguanidine or ethyl methane sulphonate, or by abortive infection by human adenovirus type 5. induced ar+ cells were fibroblastic; most grew slowly and had slightly reduced ... | 1979 | 541352 |
| replication and interaction of virus dna and cellular dna in mouse cells infected by a human adenovirus. | c57 black mouse cells infected with human adenovirus type 5 (ad5) produced large amounts of early virus proteins, small amounts of late virus proteins and less than 0.2 infectious units (i.u.)/cell of infectious virus. many cells died but the cultures recovered. virus dna and cellular dna were synthesized. some ad5 dna sedimented with cell dna in alkaline sucrose, but virus dna was rapidly lost from the culture after recovery and none of 28 unselected cloned survivors contained detectable amount ... | 1979 | 541665 |
| the influence of arginine starvation on the synthesis of virus high molecular weight dna in hela cells productively infected by adenovirus type 5. | in culture cells productively infected by adenovirus a high mol. wt. form of dna is synthesized which is known to represent, at least in part, virus dna integrated into cellular dna. we found that the synthesis of this high mol. wt. dna and the other dna size classes can strongly and differentially be influenced by altering the metabolic state of the cells. the effects of different rates of cell growth were tested in this respect as well as arginine deprivation as opposed to application of compl ... | 1979 | 541674 |
| a host range mutant of human adenovirus type 5 defective for growth in hamster cells. | a host-range mutant of adenovirus type 5, which grows in human cells but not in hamster cells, has been isolated. this mutant is complemented in mixed infection in hamster cells at 38.5 degrees c by temperature-sensitive mutants of type 5 belonging to seventeen complementation groups, and may constitute a new group. in mixed infection of human cells at 32 degrees c with the host-range and temperature-sensitive mutants, recombination takes place and by a series of two factor crosses the host-rang ... | 1978 | 567673 |
| [production and characteristics of seven rat embryo cell lines transformed by adenovirus type 5 and its dna]. | seven cell lines transformed by adenovirus type 5 and its dna were obtained. it was shown that different cell lines contain the fragments of viral dna which differ in length and number of copies per dna of diploid cells. they contain from the left end 6% of the viral dna to complete or almost complete viral genome. all studied cell lines were sensitive to reinfection with adenovirus type 5. they produced no virus being cocultivated with cell sensitive to the virus. no cell line was able to induc ... | 1979 | 571524 |
| [transcription of the genome of adenovirus type 5 in a line of transformed cells]. | | 1977 | 590087 |
| the nucleotide sequence at the termini of adenovirus type 5 dna. | the sequences of the first 194 base pairs at both termini of adenovirus type 5 (ad5) dna have been determined, using the chemical degradation technique developed by maxam and gilbert (proc. nat. acad. sci. usa 74 (1977), pp. 560-564). the nucleotide sequences 1-75 were confirmed by analysis of labeled rna transcribed from the terminal hhai fragments in vitro. the sequence data show that ad5 dna has a perfect inverted terminal repetition of 103 base pairs long. | 1977 | 600799 |
| transformation with specific fragments of adenovirus dnas. ii. analysis of the viral dna sequences present in cells transformed with a 7% fragment of adenovirus 5 dna. | five clones of rat kidney cells transformed by a small restriction endonuclease fragment of adenovirus 5 (ad5) dna (fragment hsui g, which represents the left terminal 7% of the adenovirus genome) were analyzed with respect to the viral dna sequences present in the cellular dnas. in these analyses, the kinetics of renaturation of 32p-labeled specific fragments of ad5 dna was measured in the presence of a large amount of dna extracted either from each of the transformed cell lines or from untrans ... | 1977 | 608591 |
| glucose uptake and lactic acid production of adenovirus type 5-infected hep-2 cells cultured under exponential growth and stationary phase conditions. | exponentially growing hep-2 cells have a higher rate of glucose uptake and lactic acid production than stationary phase cells. infection of cells with adenovirus type 5 stimulates glycolysis irrespective of the original rate of the host cells. therefore, infected cells cultured under exponential growth conditions have a higher rate of glycolysis than infected stationary phase cells. the rate of host cell glycolysis does not influence the time required for virus replication, the yield of infectio ... | 1977 | 617828 |
| isolation of deletion and substitution mutants of adenovirus type 5. | | 1978 | 620421 |
| characterization of adenovirus-associated virus-induced polypeptides in kb cells. | electrophoretic analysis of kb cells coinfected with adenovirus-associated virus (aav) type 2, a defective parvovirus, and adenovirus type 5 (as helper) have revealed the synthesis in vivo of at least five aav-specific polypeptides. the three largest polypeptides, with molecular weights of 90,700, 71,600, and 60,000 comigrated in polyacrylamide gels with the three aav structural polypeptides. the remaining two polypeptides had molecular weights of 24,900 and 15,800. the concentrations of the aav ... | 1978 | 621779 |
| assembly intermediates among adenovirus type 5 temperature-sensitive mutants. | temperature-sensitive mutants from three different complementation groups, ts5, ts19, and 6s58, have been shown to accumulate assembly intermediates at the restrictive temperature. the polypeptide composition of these intermediates is similar to that of the wild type, including the precursor polypeptides pvi, pvii, and pviii. ts5 and ts19 also contained cleaved precursors, indicating assembly into defective virions. the increase of infectious virus after temperature shift-down of ts19 and ts58 w ... | 1978 | 625088 |
| studies of the transcription of viral genome in adenovirus 5 transformed cells. | transcription of the human adenovirus 5 genome in transformed rat embryo cells (dfk3) was investigated using two different approaches. preferential digestion of transcribed viral sequences by dnase i was analysed using kinetics of renaturation of 32p-labeled ad5 hpai restriction fragments in the presence of material which was stable after nuclease treatment. the second approach was the hybridization of 32p-labeled nuclear rna from transformed cells with ad5 restriction fragments which were attac ... | 1978 | 643600 |
| two initiation sites for adenovirus 5.5s rna. | adenovirus specific 5.5s rna is heterogeneous at its 5' terminus. complete pancreatic rnase digests of the rna reveal a 5' terminal oligonucleotide (pp)papgpcp in addition to the major 5' terminal (pp)pgpgpgpcp (1). both 5' termini are detected early as well as late after adenovirus infection. in isolated nuclei, alpha-amanitin inhibits all 5.5s rna transcription at a concentration of 200 microgram/ml, indicating that both initiation sites are recognized by rna polymerase iii. | 1978 | 643608 |
| a second site-specific restriction endonuclease from staphylococcus aureus. | a site-specific restriction endonuclease has been isolated from staphylococcus aureus ps 96. this enzyme, sau96 i, recognizes the dna sequence 5'--g-g-n-c-c--3' and cleaves as indicated by the arrows. the enzyme 3'--c-c-n-g-g--5' cleaves adenovirus type 5 and lambda dna many times, sv40 dna 10 times and 0x174 rf dna 2 times. evidence is presented that the enzyme is involved in biological restriction-modification. | 1978 | 652518 |
| defective transforming capacity of adenovirus type 5 host-range mutants. | | 1978 | 664220 |
| synthesis of dna, late polypeptides, and infectious virus by host-range mutants of adenovirus 5 in nonpermissive cells. | | 1978 | 664263 |
| excision repair by human fibroblasts of dna damaged by r-7, t-8-dihyroxy-t-9,10-oxy-7,8,9,10- tetrahydrobenzo(a)pyrene. | benzo(a)pyrene diol-epoxide i (r-7,t-8,dihydroxy-t-9,10 oxy-7,8,9,10 tetrahydrobenzo(a)pyrene) was used to treat either human adenovirus 5 or cultures of human fibroblasts. the survival of diol-epoxide i treated adenovirus was greater when infecting fibroblasts from normal persons than when infecting fibroblasts from patients with xeroderma pigmentosum (xp). one diol-epoxide i molecule bound per viral genome correlated with one lethal hit as measured using xp fibroblasts. normal fibroblasts bloc ... | 1978 | 672921 |
| structural elements in adenovirus cores. studies by means of freeze-fracturing and ultrathin sectioning. | rod-like elements have been observed in adenovirions after freeze-fracturing of purified, semi-purified and intra-cellular adenovirus type 5. their rod-like shape was clearly demonstrated by means of stereomicrographs. ultrathin sections prepared in parallel with the freeze-fracture replicas revealed linear structures such as ribbons, rings or rods in up to 30 per cent of the semi-purified or intracellular virus particles. these observations indicate that the main 'structural element' in the ade ... | 1978 | 687118 |
| intranuclear crystalline zebra structures induced in human tumour cells by adenovirus type 5. | intranuclear crystalline inclusions with leaf-like striated appearance, i.e. zebra structures, were observed by electron microscopy in adenovirus type 5-infected human cervical and bladder carcinoma cells at 4 to 6 days after infection. | 1978 | 690614 |
| adenovirus core protein synthesis in the absence of viral dna synthesis late in infection. | the acid extraction of the adenovirus type 5 core proteins v, vii, and pvii (the precursor to vii) from infected cells and the subsequent electrophoresis on a 15% acrylamide-2.5 m urea-0.9 n acetic acid (ph 2.7) gel, revealed that peptide vii has a similar electrophoretic mobility to that of histone h1. the core proteins, which are coded by late adenovirus mrna, continued to be synthesized late in infection when viral dna synthesis was inhibited either by cytosine arabinoside in wild-type infect ... | 1978 | 691113 |
| the effect of mixed infections induced by adenovirus type 5 and vaccinia virus, on the ultrastructure of kb cells. i. ultrastructure of kb cells infected with adenovirus type 5 and vaccinia virus (within a period of 24--72 hours). | | 1978 | 708935 |
| transcription of viral sequences in cells transformed by adenovirus type 5. | | 1978 | 716215 |
| mapping of restriction sites in the transforming hpai-e fragment of adenovirus type 5 dna. | adenovirus type 5 (ad5) dna was degraded with endo r . hpai; the left-terminal fragment, hpai-e has recently been shown to be the smallest segment of ad5 dna, that can transform non-permissive cells. this fragment was labelled at its termini by limited exonuclease iii digestion followed by repair synthesis with dna polymerase and alpha-32p-labelled deoxynucleoside triphosphates. it was then further digested with each of the restriction endonucleases hpaii, haeiii, alui, hinfi and taqi; the cleav ... | 1978 | 718911 |
| mapping temperature-sensitive and host-range mutations of adenovirus type 5 by marker rescue. | | 1978 | 726266 |
| transcription of the transforming region of adenovirus type 5 in a rat cell line transformed by a small restriction fragment. | the fraction of the adenovirus type 5 genome expressed as mrna in a rat cell line, 5rk, transformed by the hsui-g restriction fragment was analyzed by saturation hybridization using separated strands of 32p-labeled hindiii-g fragment. it was found that 45 to 50% of g fragment (about 8 x 10(5) daltons) is expressed as mrna. the size of polyadenylic acid-terminated virus-specific cytoplasmic and nuclear rna was determined by electrophoresis on polyacrylamide gels containing 98% formamide, followed ... | 1978 | 731790 |
| genetic analysis of adenovirus type 2. viii. physical locations of temperature-sensitive mutations. | temperature-sensitive mutations of human adenoviruses can be physically located on the viral genome by determining the dna structures of recombinants formed in genetic crosses between different members of the same subgroup. we have analyzed the dna structures of many interserotypic recombinants formed in crosses between temperature-sensitive (ts) mutants of adenovirus type 2 and adenovirus type 5 with the restriction endonucleases bamhi, ecori, hindiii, and and sma i. in this way, we have mapped ... | 1978 | 731791 |
| the nucleotide sequence of the transforming hpai-e fragment of adenovirus type 5 dna. | the primary structure of the hpai-e fragment of adenovirus type 5 (ad5) dna has been determined, mainly by the method of maxam and gilbert (1977). this fragment comprises the leftmost 4.5% of the ad5 genome, and has been shown to be the shortest dna fragment capable of transforming cells. the identification of potential initiation and termination codons in the determined sequence indicates that two small polypeptides consisting of 186, and 81 amino acids, respectively, could be synthesized. taki ... | 1978 | 744489 |
| mapping of adenovirus type 5 temperature-sensitive mutations by marker rescue in enhanced double dna infections. | adenovirus type 5 dna has low infectivity (graham & van der eb, 1973) which can be increased by various techniques, one of which is the dimethyl sulphoxide (dmso) boost (stow & wilkie, 1976). in this report, it is shown that dmso treatment of adenovirus 5 dna-infected hela cells results in a 10-fold increase in plaque formation, and that this can be used to facilitate marker rescue experiments. double dna infections were performed by the calcium phosphate method, co-precipitating intact temperat ... | 1978 | 745009 |
| [extraction of adenovirus type 5 dna fragments using specific e. coli endonuclease]. | | 1976 | 785234 |
| localization and organization of microfilaments and related proteins in normal and virus-transformed cells. | the localization and organization of actin-like microfilaments in normal, sv-40 and adenovirus transformed cells are determined by the coordinated use of light optical, electron optical and biochemical techniques. in adenovirus-type 5 transformed hamster embryo cells, microfilament meshworks appear to be the predominant organizational form of cellular action, while in normal hamster cells, microfilament bundles are prevalent. differences between 3t3 and sv-40 transformed 3t3 cells are less appar ... | 1976 | 794589 |
| [immunofluorescence study of the migration of adenovirus 5 in hela cell cytoplasm during the penetration phase]. | using the indirect immunofluorescence technique in presence of anti-adenovirus-5 antibody, fluorescent spots were observed two hours after adsorption at 4 degrees c onto hela cells on the surface of the cells. the spots subsequently became intracytoplasmic and persisted about 7 to 8 h. intense fluorescence was further observed in the whole cytoplasm and in the nucleus 12 h following the injection. | 1975 | 807382 |
| electron microscopy of adenovirus cores. | adenovirus type 5 'cores' prepared by heating in the presence of deoxycholate and partially purified on a glycerol density gradient could be visualized as roughly isometrical particles with a condensed centre from which twisted filaments or loops of dna emanated. this compact structure was readily dispersed by spreading on distilled water or by treatment with edta, nonidet, dnase or trypsin. spreading with nonidet was particularly effective in unfolding the cores and revealing long filaments abo ... | 1975 | 808587 |
| an adenovirus type 5 gene function required for initiation of viral dna replication. | | 1975 | 810963 |
| polypeptide phosphorylation in adenovirus-infected cells. | cells infected with human adenovirus type 5 have been labelled with 32p-orthophosphate under various conditions and extracts examined, after denaturation in sodium dodecyl sulphate (sds), by polyacrylamide gel electrophoresis (page) followed by autoradiography. a number of polypeptides appear to be phosphorylated specifically as a result of infection. early in infection, phosphorylation of a polypeptide of apparent mol. wt. 26 k associated with ribosomes can be detected. two other phosphorylated ... | 1977 | 833577 |
| physical properties of nucleoprotein cores from adenovirus type 5. | analytical ultracentrifugation, thermal denaturation, and electron microscopy have been used to study nucleoprotein core particles, obtained from disrupted type 5 adenovirus and partially purified on glycerol density gradients. electron microscopy at low salt concentrations has shown that the cores are homogeneous particles with characteristic structures, which vary with conditions of observation from a fairly loose network of fibers to a highly condensed, compact particle. sedimentation measure ... | 1977 | 840648 |
| host-range mutants of adenovirus type 5 defective for growth in hela cells. | | 1977 | 841862 |
| crystallization and reaggregation of adenovirus type 5 structural components from infected cell extracts. | treatment of adenovirus type 5-infected cells at late times after infection (48 to 72 h p.i.) by hypotonic buffer and subsequent manipulation of the crude extract has resulted in the crystallization and reaggregation of the adenovirus type 5 fibre and hexon components. | 1977 | 853292 |
| deoxyribonuclease activity associated with adenovirus 5 and 7. | a dnase activity was found associated with isolated components of adenovirus types 5 and 7. the enzymatic activity was associated with the purified virus and viral components extracted from the soluble material of infected cells. the dnase activity of adenovirus type 5 was maximum at ph 5.5 and 7.0 whereas adenovirus 7 was active only at ph 7.0. both dnases were shown to be located in the penton fraction. | 1977 | 856583 |
| dna polymerases in adenovirus type 5-infected and uninfected kb cells. induction of an alpha-type dna polymerase in adenovirus type 5-infected and in fast growing cells. | dna polymerase activities in uninfected kb cells or kb cells infected with adenovirus type 5 (ad5) were compared by chromatography on dna-cellulose and deae-cellulose and by isoelectric focusing. on dna cellulose three components were found both in infected and in uninfected cells. the major component eluted at 0.15 m nac1 and contained dna polymerase alpha. two minor components were found, one which did not bind to dna-cellulose and one which bound strongly. this latter component contained dna ... | 1977 | 861228 |