| pathways of d-fructose and d-glucose catabolism in marine species of alcaligenes, pseudomonas marina, and alteromonas communis. | cell-free extracts of d-fructose grown cells of marine species of alcaligenes as well as pseudomonas marina contained an activity which catalyzed a p-enolpyruvate-dependent phosphorylation of d-fructose in the 1-position as well as activities of the following enzymes: 1-p-fructokinase, fructose-1,6-p2 aldolase, ppi-dependent 6-p-fructokinase, fructokinase, glucokinase, p-hexose isomerase, glucose-6-p dehydrogenase, 6-p-gluconate dehydrase, and 2-keto-3-deoxy-6-p-gluconate aldolase. the presence ... | 1977 | 139858 |
| purification and properties of restriction endonuclease from deleya marina iam 14114, a marine bacterium (dmai). | a restriction endonuclease, designated as dmai, was purified from cell-free extracts of deleya marina iam 14114 by streptomycin treatment, ammonium sulfate fractionation and two steps of chromatographies on heparin-sepharose cl-6b and mono q (hr 5/5, fplc). the purified enzyme was homogeneous on sds-polyacrylamide gel disk electrophoresis and a ligation-recutting test. the relative molecular mass measurements of the purified enzyme gave 28,000 daltons by sds-polyacrylamide gel disk electrophores ... | 1990 | 1369311 |
| comparison of the adhesion properties of deleya marina and the exopolysaccharide-defective mutant strain dmr. | deleya marina 219 (atcc 25374) produces large quantities of an acidic exopolysaccharide and characteristically forms mucoid colonies and large aggregates of cells. the exopolysaccharide of wild-type d. marina cells appears to occur as both film and fibrils in electron micrographs. the organization of exopolymeric material was indicative of structural heterogeneity. a spontaneous rough-colony mutant defective in exopolysaccharide, d. marina dmr, has been isolated. the absence of exopolymer corres ... | 1991 | 1781675 |
| the effect of nickel on a marine bacterium, arthrobacter marinus sp.nov. | | 1970 | 5493594 |
| the effect of nickel on a marine bacterium: fine structure of arthrobacter marinus. | | 1971 | 5571860 |
| assimilatory sulfur metabolism in marine microorganisms: characteristics and regulation of sulfate transport in pseudomonas halodurans and alteromonas luteo-violaceus. | sulfate transport capacity was not regulated by cysteine, methionine, or glutathione in pseudomonas halodurans, but growth on sulfate or thiosulfate suppressed transport. subsequent sulfur starvation of cultures grown on all sulfur sources except glutathione stimulated uptake. only methionine failed to regulate sulfate transport in alteromonas luteo-violaceus, and sulfur starvation of all cultures enhanced transport capacity. during sulfur starvation of sulfate-grown cultures of both bacteria, t ... | 1981 | 7263610 |
| assimilatory sulfur metabolism in marine microorganisms: a novel sulfate transport system in alteromonas luteo-violaceus. | the sulfate transport mechanism of a marine bacterium, alteromonas luteo-violaceus, was unique among microorganisms in its extremely low affinity for the sulfate analog thiosulfate. distinguishing characteristics included weak inhibition of sulfate transport by thiosulfate, inability to transport thiosulfate effectively, poor growth using thiosulfate as the sole source of sulfur, and a mild effect of the sulfhydryl reagent para-hydroxymercuribenzoate. in contrast, sulfate transport by a marine p ... | 1981 | 7263611 |
| development of a gene reporter system in moderately halophilic bacteria by employing the ice nucleation gene of pseudomonas syringae. | the expression of the ice nucleation gene inaz of pseudomonas syringae in several moderate halophiles was investigated to establish its utility as a reporter for promoter activity and gene expression studies in these biotechnologically and environmentally important bacteria. a promoterless version of inaz was introduced in two different restriction sites and at both orientations in a recombinant plasmid able to replicate in moderate halophiles and, in particular, within the sequence of its phe1 ... | 1995 | 8526492 |
| description of two new species of halomonas: halomonas israelensis sp.nov. and halomonas canadensis sp.nov. | six well-known strains of halotolerant bacteria, including two strains previously identified only as nrcc 41227 and ba1, have been compared using 125 phenotypic characters and dna-dna hybridization. although these strains represent some of the most heavily studied salt-tolerant bacteria, they have never been taxonomically compared. the data presented show that these bacteria form a relatively homogeneous group related at the genus level. the taxonomic comparison showed that these six organisms r ... | 1995 | 8542554 |
| attachment of bacteria to model solid surfaces: oligo(ethylene glycol) surfaces inhibit bacterial attachment. | bacterial cell attachment to the surfaces of self-assembled monolayers formed by the adsorption of omega-substituted alkanethiols on transparent gold films has been studied under defined bacterial culture and flow conditions. phase contrast microscopy was used to quantify the attachment of two organisms, one of medical (staphylococcus epidermidis) and one of marine (deleya marina) importance. self-assembled monolayers terminated with hexa(ethylene glycol), methyl, carboxylic acid and fluorocarbo ... | 1996 | 8759791 |
| n-acyl amino acid biosynthesis in marine bacterium, deleya marina. | we reported previously that the marine bacterium, deleya marina (atcc 25374), produced n-acyl leucine and isoleucine, in which nonhydroxy fatty acid was linked to alpha-amino group of amino acid. further analysis of bacterium lipids revealed the additional production of n-acyl ornithine. the n-acyl ornithine had a 3-hydroxy fatty acid linked by an amide bond to a-amino group of ornithine and a nonhydroxy fatty acid esterified to the hydroxy group of the 3-hydroxy fatty acid. n-acyl ornithine was ... | 1997 | 9271247 |
| novel diglycosyldiacylglycerol from the gram-negative bacterium deleya marina. | a glycosyldiacylglycerol was isolated from the marine bacterium deleya marina (atcc 25374). the structure was determined, mainly by spectral data, to be 1, 2-diacyl-3-o-[alpha-2-amino-2-deoxy-glucopyranose-(1-->4)-o-alpha-idu ronopyranuronic acid]-glycerol. this is, to our knowledge, the first isolation of diglycosyldiacylglycerol containing both iduronopyranuronic acid and 2-amino-2-deoxy-glucopyranose from gram-negative bacteria. | 1998 | 9714788 |
| surface-grafted, environmentally sensitive polymers for biofilm release. | controlling bacterial biofouling is desirable for almost every human enterprise in which solid surfaces are introduced into nonsterile aqueous environments. one approach that is used to decrease contamination of manufactured devices by microorganisms is using materials that easily slough off accumulated material (i.e., fouling release surfaces). the compounds currently used for this purpose rely on low surface energy to inhibit strong attachment of organisms. in this study, we examined the possi ... | 1999 | 10103257 |
| importance of passive diffusion in the uptake of polychlorinated biphenyls by phagotrophic protozoa. | unicellular protozoan grazers represent a size class of organisms where a transition in the mechanism of chlorobiphenyl (cb) introduction, from diffusion through surface membranes to ingestion of contaminated prey, could occur. this study compares the relative importance of these two processes in the overall uptake of polychlorinated biphenyls by protists. uptake rates and steady-state concentrations were compared in laboratory cultures of grazing and nongrazing protozoa. these experiments were ... | 2000 | 10788371 |
| marine protozoa produce organic matter with a high affinity for pcbs during grazing. | processes influencing organic carbon distribution and composition can control the speciation of organic contaminants such as polychlorinated biphenyls (pcbs) and ultimately determine their residence time in aquatic environments. protozoan grazers are active in the remineralization and recycling of organic material both in the water column and at the sediment-water interface. thus, they influence the quality and quantity of potential pcb binding substrates in the suspended and dissolved phases of ... | 2001 | 11686367 |
| phylogeny of the family halomonadaceae based on 23s and 165 rdna sequence analyses. | in this study, we have evaluated the phylogenetic status of the family halomonadaceae, which consists of the genera halomonas, chromohalobacter and zymobacter, by comparative 23s and 16s rdna analyses. the genus halomonas illustrates very well a situation that occurs often in bacterial taxonomy. the use of phylogenetic tools has permitted the grouping of several genera and species believed to be unrelated according to conventional taxonomic techniques. in addition, the number of species of the g ... | 2002 | 11837309 |
| analysis and expression of algl, which encodes alginate lyase in pseudomonas syringae pv. syringae. | pseudomonas syringae produces alginate, an exopolysaccharide that contributes to the virulence and epiphytic fitness of this phytopathogenic bacterium. p. syringae also produces the algl-encoded alginate lyase, which cleaves the alginate biopolymer via a beta-elimination reaction. the algl gene from p. syringae maps to a 1134 bp region within the alginate biosynthetic operon, and is similar to algl from halomonas marina, p. aeruginosa, azotobacter chroococcum, and a. vinelandii. algl from p. syr ... | 2001 | 11913796 |
| two species of culturable bacteria associated with degradation of brown algae fucus evanescens. | the heterotrophic microbial enrichment community established during degradation of brown algae fucus evanescens was characterized. a two-species bacterial community of marine culturable gamma-proteobacteria consisted of pseudoalteromonas and halomonas. the first member of the community, pseudoalteromonas sp., was highly metabolically active, had bacteriolytic and hemolytic activities, produced proteinases (gelatinase and caseinase), lipases, dnases, and fucoidanhydrolases, laminaranases, alginas ... | 2002 | 12023731 |
| use of pcr-rflp for genotyping 16s rrna and characterizing bacteria cultured from halibut fry. | small subunit ribosomal genes were explored using pcr-rflp to facilitate the characterization of bacteria cultured from reared fry of the atlantic halibut (hippoglossus hippoglossus). concern has been expressed about pathogen invasion in larvae lacking a counteracting normal flora that may aid the immune system in producing robust noninfected individuals. in this study, pure cultured representatives of normal flora that were previously found to be antagonistic towards a pathogenic vibrio sp. wer ... | 2002 | 12109877 |
| proposal of cobetia marina gen. nov., comb. nov., within the family halomonadaceae, to include the species halomonas marina. | at present the family halomonadaceae consists of four genera and the taxonomic status of all species included in these genera is not equally supported by phylogenetic evidence. whereas zymobacter and carnimonas contain one single species each and form the deeper branch of the family, the genus halomonas contains currently 22 species, with a maximum 16s/23s rrna sequence divergence greater than it could be expected for species of the same genus. in addition, we have witnessed during last years an ... | 2002 | 12353874 |
| bacterial influences on atlantic halibut hippoglossus hippoglossus yolk-sac larval survival and start-feed response. | a bacteria-free halibut larval rearing system was used to test 20 bacterial isolates, from british halibut hatcheries, for their toxicity towards halibut yolk-sac larvae under microbially controlled conditions. the isolates tested spanned a range of genera and species (pseudoalteromonas, halomonas marina, vibrio salmonicida-like, photobacterium phosphoreum and v. splendidus species). a pathogen of turbot, scophthalmus maximus, v. anguillarum 91079, and 2 isolates from adult halibut were also inc ... | 2003 | 14598986 |
| temperature and nutrient availability control growth rate and fatty acid composition of facultatively psychrophilic cobetia marina strain l-2. | a facultative psychrophilic bacterium, strain l-2, that grows at 0 and 5 degrees c as minimum growth temperatures in complex and defined media, respectively, was isolated. on the basis of taxonomic studies, strain l-2 was identified as cobetia marina. the adaptability of strain l-2 to cold temperature was higher than that of the type strain and of other reported strains of the same species. when the bacterium was grown at 5-15 degrees c in a defined medium, it produced a high amount of trans-uns ... | 2004 | 15067498 |
| effect of substratum surface chemistry and surface energy on attachment of marine bacteria and algal spores. | two series of self-assembled monolayers (sams) of omega-substituted alkanethiolates on gold were used to systematically examine the effects of varying substratum surface chemistry and energy on the attachment of two model organisms of interest to the study of marine biofouling, the bacterium cobetia marina (formerly halomonas marina) and zoospores of the alga ulva linza (formerly enteromorpha linza). sams were formed on gold-coated glass slides from solutions containing mixtures of methyl- and c ... | 2004 | 15240295 |
| a highly active alkaline phosphatase from the marine bacterium cobetia. | an alkaline phosphatase with unusually high specific activity has been found to be produced by the marine bacterium cobetia marina (strain kmm mc-296) isolated from coelomic liquid of the mussel crenomytilus grayanus. the properties of enzyme, such as a very high specific activity (15000 de u/1 mg of protein), no activation with divalent cations, resistance to high concentrations of inorganic phosphorus, as well as substrate specificity toward 5' nucleotides suggest that the enzyme falls in an i ... | 2005 | 15906116 |
| halomonas taeanensis sp. nov., a novel moderately halophilic bacterium isolated from a solar saltern in korea. | a moderately halophilic, gram-negative bacterium, strain bh539(t), which was isolated from a solar saltern at taean in korea, was considered to be a member of the genus halomonas. strain bh539(t) grew at salinities of 1-25% (w/v) and at temperatures of 10-45 degrees c. cells were short rods that were motile by means of several flagella. their major fatty acids were c(18:1)omega7c, c(16:0) and c(19:0) cyclo omega8c. the genomic dna g+c content was about 65 mol% and the predominant ubiquinone was ... | 2005 | 16166705 |
| reversible protein adsorption and bioadhesion on monolayers terminated with mixtures of oligo(ethylene glycol) and methyl groups. | surface-grafted, environmentally responsive polymers have shown great promise for controlling adsorption and desorption of macromolecules and cells on solid surfaces. in the paper, we demonstrate that certain mixed self-assembled monolayers (sams) of oligo(ethylene glycol) (oeg) and methyl-terminated alkanethiolates on gold form surfaces with switchable hydrophobicity and tendency for protein adsorption and cellular attachment. at temperatures above 32 degrees c, sams with a surface density of a ... | 2005 | 16231888 |
| assimilatory sulfur metabolism in marine microorganisms: sulfur metabolism, protein synthesis, and growth of alteromonas luteo-violaceus and pseudomonas halodurans during perturbed batch growth. | the antibiotic protein synthesis inhibitor chloramphenicol specifically blocked the incorporation of [s]sulfate into the residue protein of two marine bacteria, pseudomonas halodurans and alteromonas luteo-violaceus. simultaneous inhibition of total protein synthesis occurred, but incorporation of s into low-molecular-weight organic compounds continued. a. luteo-violaceus rapidly autolyzed, with similar reduction in cell counts, total culture protein and cellular sulfur, whereas p. halodurans re ... | 1982 | 16345918 |
| assimilatory sulfur metabolism in marine microorganisms: considerations for the application of sulfate incorporation into protein as a measurement of natural population protein synthesis. | the sulfur content of residue protein was determined for pure cultures of nitrosococcus oceanus, desulfovibrio salexigens, 4 mixed populations of fermentative bacteria, 22 samples from mixed natural population enrichments, and 11 nutritionally and morphologically distinct isolates from enrichments of sargasso sea water. the average 1.09 +/- 0.14% (by weight) s in protein for 13 pure cultures agrees with the 1.1% calculated from average protein composition. an operational value encompassing all m ... | 1982 | 16345919 |
| interactions between shewanella colwelliana, oyster larvae, and hydrophobic organophosphate pesticides. | shewanella colwelliana (strain d) is a periphytic estuarine bacterium that forms biofilms beneficial to oyster set. our study examined whether these and other films concentrated two hydrophobic, organophosphate pesticides, abate and malathion, that are detected in chesapeake bay oyster waters. both biofilms and purified exopolysaccharide of s. colwelliana did not adsorb more of the abate or malathion than could be accounted for by adsorption to control surfaces. similar results were obtained by ... | 1990 | 16348382 |
| the role of vanadium in the chemical defense of the solitary tunicate, phallusia nigra. | ascidians (sea squirts) may defend themselves from predators, biofouling competitors, and bacterial infection by producing secondary metabolites or sequestering acid, but many species also accumulate heavy metals, most notably vanadium. the defensive functions of heavy metals in ascidians remain unclear, and to this end, the solitary caribbean tunicate, phallusia nigra, was studied to localize vanadium in its tissues and to assess the defensive properties of vanadium-containing compounds. as det ... | 2007 | 17265174 |
| chemical defence in mussels: antifouling effect of crude extracts of the periostracum of the blue mussel mytilus edulis. | shells of the blue mussel mytilus edulis remain free of fouling organisms as long as they possess an intact periostracum, and a multiple antifouling defence that comprises a ripple-like microtopography and the production of chemical antifouling compounds has been suggested previously. this study investigates the chemical defence strategy of blue mussels for the first time. six crude extracts of the periostracum of intact shells were made using solvents of increasing polarity. these extracts were ... | 2006 | 17290869 |
| combinatorial materials research applied to the development of new surface coatings iii. utilisation of a high-throughput multiwell plate screening method to rapidly assess bacterial biofilm retention on antifouling surfaces. | the authors recently reported on the development of a novel multiwell plate screening method for the high-throughput assessment of bacterial biofilm retention on surfaces. two series of biocide containing coatings were prepared to assess the ability of the developed assay to adequately discern differences in antifouling performance: i) a commercially available poly(methyl methacrylate) (pmma) and silicone elastomer (dc) physically blended with an organic antifouling biocide sea-nine 211 (sn211) ... | 2007 | 17453727 |
| evaluation of the activity of the sponge metabolites avarol and avarone and their synthetic derivatives against fouling micro- and macroorganisms. | the sesquiterpene hydroquinone avarol (1) was isolated from the marine sponge dysidea avara, whereas the corresponding quinone, avarone (2), was obtained by oxidation of avarol, and the significantly more lipophilic compounds [3'-(p-chloro-phenyl)avarone (3), 3',4'-ethylenedithioavarone (4), 4'-isopropylthioavarone (5), 4'-tert-butylthioavarone (6), 4'-propylthioavarone (7), 4'-octylthioavarone (8)] were obtained by nucleophilic addition of thiols or p-chloroaniline to avarone. all these compoun ... | 2007 | 17873837 |
| antifouling activity of sessile bacilli derived from marine surfaces. | marine biofilms are a virtually untapped source of bioactive molecules that may find application as novel antifoulants in the marine paint industry. this study aimed at determining the potential of marine biofilm bacteria to produce novel biomolecules with potential application as natural antifoulants. nine representative strains were isolated from a range of surfaces and were grown in yeb medium and harvested during the late exponential growth phase. bacterial biomass and spent culture medium w ... | 2008 | 17909869 |
| light-induced biocidal action of conjugated polyelectrolytes supported on colloids. | a series of water soluble, cationic conjugated polyelectrolytes (cpes) with backbones based on a poly(phenylene ethynylene) repeat unit structure and tetraakylammonium side groups exhibit a profound light-induced biocidal effect. the present study examines the biocidal activity of the cpes, correlating this activity with the photophysical properties of the polymers. the photophysical properties of the cpes are studied in solution, and the results demonstrate that direct excitation produces a tri ... | 2008 | 18729335 |
| poly(ethylene glycol)-containing hydrogel surfaces for antifouling applications in marine and freshwater environments. | this work describes the fabrication, characterization, and biological evaluation of a thin protein-resistant poly(ethylene glycol) (peg)-based hydrogel coating for antifouling applications. the coating was fabricated by free-radical polymerization on silanized glass and silicon and on polystyrene-covered silicon and gold. the physicochemical properties of the coating were characterized by infrared spectroscopy, ellipsometry, and contact angle measurements. in particular, the chemical stability o ... | 2008 | 18759475 |
| production of poly-3-hydroxyalkanoic acids by a moderately halophilic bacterium, halomonas marina hma 103 isolated from solar saltern of orissa, india. | halomonas marina hma 103 (mtcc 8968), the moderately halophilic bacterium isolated and characterized from the solar saltern of orissa, india, grows optimally at 10% (w/v) nacl in culture medium and is able to synthesize poly(3-hydroxybutyrate) [p(3hb)] during growth. this study is an attempt to optimize the cultural conditions for efficient production of p(3hb) by h. marina in batch cultivation. growth of the organism under shake-flask culture using 2% (w/v) glucose resulted in p(3hb) accumulati ... | 2009 | 19621765 |
| cobetia crustatorum sp. nov., a novel slightly halophilic bacterium isolated from traditional fermented seafood in korea. | a slightly halophilic, gram-stain-negative, straight-rod-shaped aerobe, strain jo1(t), was isolated from jeotgal, a traditional korean fermented seafood. cells were observed singly or in pairs and had 2-5 peritrichous flagella. optimal growth occurred at 25 degrees c, in 6.5 % (w/v) salts and at ph 5.0-6.0. strain jo1(t) was oxidase-negative and catalase-positive. cells did not reduce fumarate, nitrate or nitrite on respiration. acid was produced from several carbohydrates and the strain utilize ... | 2010 | 19654339 |
| marine bacterial organisation around point-like sources of amino acids. | abstract to better understand the trigger for and use of motility in marine bacterial chemotaxis, specific (amino acids) chemical stimuli were used to test the effect on bacterial speed and reorientation. an assay system was developed to analyse bacterial behavioural responses to point-like nutrient sources (beads 10-40 mum). the marine bacteria pseudoalteromonas haloplanktis, shewanella putrefaciens, deleya marina, the enteric bacterium escherichia coli and an enriched assemblage of marine bact ... | 2003 | 19719700 |
| bacterial assay for the rapid assessment of antifouling and fouling release properties of coatings and materials. | an assay has been developed to accurately quantify the growth and release behaviour of bacterial biofilms on several test reference materials and coatings, using the marine bacterium cobetia marina as a model organism. the assay can be used to investigate the inhibition of bacterial growth and release properties of many surfaces when compared to a reference. the method is based upon the staining of attached bacterial cells with the nucleic acid-binding, green fluorescent syto 13 stain. a strong ... | 2010 | 20039190 |
| [hydroxyectoine synthesis and release under osmotic shock in cobetia marina cicc10367]. | to obtain hydroxyectoine-producing strain with tolerance to osmotic shock and improve hydroxyectoine productivity by adopting "bacteria milking" process. | 2009 | 20222443 |
| conjugated polyelectrolyte capsules: light-activated antimicrobial micro "roach motels". | microcapsules consisting of alternating layers of oppositely charged poly(phenylene ethynylene)-type conjugated polyelectrolytes (cpes) were prepared via layer-by-layer deposition onto mnco3 template particles followed by dissolution of the template particles using an ethylenediaminetetraacetate solution. the resulting microcapsules exhibit bright-green fluorescence emission characteristics of the cpes. strong antimicrobial activity was observed upon mixing of polyelectrolyte capsules with cobet ... | 2009 | 20355752 |
| poly(ethylene glycol)-based thiol-ene hydrogel coatings-curing chemistry, aqueous stability, and potential marine antifouling applications. | photocured thiol-ene hydrogel coatings based on poly(ethylene glycol) (peg) were investigated for marine antifouling purposes. by varying the peg length, vinylic end-group, and thiol cross-linker, a library of hydrogel coatings with different structural composition was efficiently accomplished, with or without ester linkages. the thiol-methacrylate and thiol-allyl systems were evaluated with respect to curing, degradation, as well as antifouling properties. methacrylate-based systems exhibited h ... | 2010 | 20356297 |
| engineered antifouling microtopographies: the role of reynolds number in a model that predicts attachment of zoospores of ulva and cells of cobetia marina. | a correlation between the attachment density of cells from two phylogenetic groups (prokaryotic bacteria and eukaryotic plantae), with surface roughness is reported for the first time. the results represent a paradigm shift in the understanding of cell attachment, which is a critical step in the biofouling process. the model predicts that the attachment densities of zoospores of the green alga, ulva, and cells of the marine bacterium, cobetia marina, scale inversely with surface roughness. the s ... | 2010 | 20706891 |
| the cq ratio of surface energy components influences adhesion and removal of fouling bacteria. | the interaction energy between bacteria and substrata with different surface energies was modelled by the extended dlvo-á(derjaguin, landau, verwey and overbeek) theory. the modeling results revealed that the interaction energy has a strong correlation with the cq (chen and qi) ratio, which is defined as the ratio of the lifshitz-van der waals (lw) apolar to the electron donor surface energy components of substrata. both modeling and experimental results with different bacteria including p. fluo ... | 2011 | 21390912 |
| antifouling performance of cross-linked hydrogels: refinement of an attachment model. | poly(ethylene glycol) dimethacrylate (pegdma), pegdma-co-glycidyl methacrylate (pegdma-co-gma), and pegdma-co-hydroxyethyl methacrylate (pegdma-co-hema) hydrogels were polymerized using ammonium persulfate and ascorbic acid as radical initiators. surface energies of the hydrogels and a standard, poly(dimethylsiloxane) elastomer (pdmse), were characterized using captive bubble and sessile drop measurements, respectively (? = 52 mn/m, ?(0) = 19 mn/m). the chemical composition of the hydrogels was ... | 2011 | 21401017 |
| x-ray holographic microscopy with zone plates applied to biological samples in the water window using 3rd harmonic radiation from the free-electron laser flash. | the imaging of hydrated biological samples - especially in the energy window of 284-540 ev, where water does not obscure the signal of soft organic matter and biologically relevant elements - is of tremendous interest for life sciences. free-electron lasers can provide highly intense and coherent pulses, which allow single pulse imaging to overcome resolution limits set by radiation damage. one current challenge is to match both the desired energy and the intensity of the light source. we presen ... | 2011 | 21716334 |
| resistance of galactoside-terminated alkanethiol self-assembled monolayers to marine fouling organisms. | self-assembled monolayers (sams) of galactoside-terminated alkanethiols have protein-resistance properties which can be tuned via the degree of methylation [langmuir 2005, 21, 2971-2980]. specifically, a partially methylated compound was more resistant to nonspecific protein adsorption than the hydroxylated or fully methylated counterparts. we investigate whether this also holds true for resistance to the attachment and adhesion of a range of marine species, in order to clarify to what extent re ... | 2011 | 21916438 |
| cloning and expression of a highly active recombinant alkaline phosphatase from psychrotrophic cobetia marina. | alkaline phosphatase catalyzes the hydrolysis of phosphomonoesters and is widely used in molecular biology techniques and clinical diagnostics. we expressed a recombinant alkaline phosphatase of the marine bacterium, cobetia marina, in escherichia coli bl21 (de3). the recombinant protein was purified with a specific activity of 12,700 u/mg protein, which is the highest activity reported of any bacterial alkaline phosphatase studied to date. the molecular mass of the recombinant protein was 55-60 ... | 2011 | 22009571 |
| hybrid hairy janus particles as building blocks for antibiofouling surfaces. | herein, we report a new strategy for the design of antifouling surfaces by using hybrid hairy janus particles. the amphiphilic janus particles possess either a spherical or a plateletlike shape and have core-shell structures with an inorganic core and hydrophilic/hydrophobic polymeric shells. subsequently, these bifunctional janus particles enable the fabrication of surfaces with modularity in chemical composition and final surface topography, which possess antifouling properties. the antifoulin ... | 2016 | 27933847 |
| data supporting functional diversity of the marine bacterium cobetia amphilecti kmm 296. | data is presented in support of functionality of hyper-diverse protein families encoded by the cobetia amphilecti kmm 296 (formerly cobetia marina kmm 296) genome ("the genome of the marine bacterium cobetia marina kmm 296 isolated from the mussel crenomytilus grayanus (dunker, 1853)" [1]) providing its nutritional versatility, adaptability and biocontrol that could be the basis of the marine bacterium evolutionary and application potential. presented data include the information of growth and b ... | 2016 | 27508225 |
| charged hydrophilic polymer brushes and their relevance for understanding marine biofouling. | the resistance of charged polymers to biofouling was investigated by subjecting cationic (pdmaema), anionic (pspma), neutral (phema-co-peg10ma), and zwitterionic (psbma) brushes to assays testing protein adsorption; attachment of the marine bacterium cobetia marina; settlement and adhesion strength of zoospores of the green alga ulva linza; settlement of barnacle (balanus amphitrite and b. improvisus) cypris larvae; and field immersion tests. several results go beyond the expected dependence on ... | 2016 | 27125564 |
| mevalonosomes: specific vacuoles containing the mevalonate pathway in plocamium brasiliense cortical cells (rhodophyta). | this paper has identified, for the first time in a member of the rhodophyta, a vacuolar organelle containing enzymes that are involved in the mevalonate pathway-an important step in red algal isoprenoid biosynthesis. these organelles were named mevalonosomes (mev) and were found in the cortical cells (cc) of plocamium brasiliense, a marine macroalgae that synthesizes several halogenated monoterpenes. p. brasiliense specimens were submitted to a cytochemical analysis of the activity of the 3-hydr ... | 2015 | 26986518 |
| effective harvesting of the microalgae chlorella vulgaris via flocculation-flotation with bioflocculant. | in this study, bioflocculant from cobetia marina l03 could be used for effective harvesting of the microalgae chlorella vulgaris via flocculation-flotation. a flotation efficiency of 92.7% was observed when 20 mg l(-1) bioflocculant was tested for flocculating the microalgal cells with 5mm cacl2. the bioflocculant was stable at wide ranges of ph and temperature, which is advantageous for its application under various conditions. chemical analysis of the bioflocculant indicated that it is compose ... | 2015 | 26391967 |
| the marine bacteria cobetia marina dsmz 4741 synthesizes an unexpected k-antigen-like exopolysaccharide. | we have studied the exopolysaccharide produced by cobetia marina dsmz 4741, a marine bacterium isolated from coastal seawater. this strain is able to produce a polysaccharide in presence of carbon sources as glucose, mannitol and alginate. the maximum production occurs in aerobic condition, during the end of the exponential phase. the polymer is a non-viscous, acidic heteropolysaccharide of 270kda constituted of a repeating unit of: this kind of chemical structure is generally related to k-antig ... | 2015 | 25839829 |
| a novel bifunctional hybrid with marine bacterium alkaline phosphatase and far eastern holothurian mannan-binding lectin activities. | a fusion between the genes encoding the marine bacterium cobetia marina alkaline phosphatase (cmap) and far eastern holothurian apostichopus japonicus mannan-binding c-type lectin (mbl-aj) was performed. expression of the fusion gene in e. coli cells resulted in yield of soluble recombinant chimeric protein cmap/mbl-aj with the high alkaline phosphatase activity and specificity of the lectin mbl-aj. the bifunctional hybrid cmap/mbl-aj was produced as a dimer with the molecular mass of 200 kda. t ... | 2014 | 25397876 |
| recombinant production and characterization of a highly active alkaline phosphatase from marine bacterium cobetia marina. | the psychrophilic marine bacterium, cobetia marina, recovered from the mantle tissue of the marine mussel, crenomytilus grayanus, which contained a gene encoding alkaline phosphatase (ap) with apparent biotechnology advantages. the enzyme was found to be more efficient than its counterparts and showed k cat value 10- to 100-fold higher than those of all known commercial aps. the enzyme did not require the presence of exogenous divalent cations and dimeric state of its molecule for activity. the ... | 2015 | 25260971 |
| hydration and chain entanglement determines the optimum thickness of poly(hema-co-peg₁₀ma) brushes for effective resistance to settlement and adhesion of marine fouling organisms. | understanding how surface physicochemical properties influence the settlement and adhesion of marine fouling organisms is important for the development of effective and environmentally benign marine antifouling coatings. we demonstrate that the thickness of random poly(hema-co-peg10ma) copolymer brushes affect antifouling behavior. films of thicknesses ranging from 50 to 1000 å were prepared via surface-initiated atom-transfer radical polymerization and characterized using infrared spectroscopy, ... | 2014 | 24945705 |
| surface anchored metal-organic frameworks as stimulus responsive antifouling coatings. | surface-anchored, crystalline and oriented metal organic frameworks (surmofs) have huge potential for biological applications due to their well-defined and highly-porous structure. in this work we describe a mof-based, fully autonomous system, which combines sensing, a specific response, and the release of an antimicrobial agent. the cu-containing surmof, cu-surmof 2, is stable in artificial seawater and shows stimulus-responsive anti-fouling properties against marine bacteria. when cobetia mari ... | 2013 | 24706148 |
| thermodynamic analysis of marine bacterial attachment to oligo(ethylene glycol)-terminated self-assembled monolayers. | colloidal models are frequently used to model the thermodynamics of bacterial attachment to surfaces. the most commonly used of such models is that proposed by van oss, chaudhury and good, which includes both non-polar and polar (including hydrogen bonding) interactions between the attaching bacterium, the attachment substratum and the aqueous environment. we use this model to calculate the free energy of adhesion, ∆gadh, for attachment of the marine bacterium cobetia marina to well defined atta ... | 2013 | 24706137 |
| [microbial diversity of pyropia haitanensis phycosphere during cultivation]. | pyropia haitanensis is of great commercial importance and wildly cultivated in zhejiang and fujian provinces. to observe the characteristics and changes of phycosphere microbial communities during cultivation can help us monitor the potential pathogens and microbial factors affecting the health of cultivated seaweeds. | 2013 | 24409765 |
| anti-biofilm performance of three natural products against initial bacterial attachment. | marine bacteria contribute significantly towards the fouling consortium, both directly (modern foul release coatings fail to prevent "slime" attachment) and indirectly (biofilms often excrete chemical cues that attract macrofouling settlement). this study assessed the natural product anti-biofilm performance of an extract of the seaweed, chondrus crispus, and two isolated compounds from terrestrial sources, (+)-usnic acid and juglone, against two marine biofilm forming bacteria, cobetia marina a ... | 2013 | 24192819 |
| engineered antifouling microtopographies: an energetic model that predicts cell attachment. | we have developed a model for the prediction of cell attachment to engineered microtopographies based on two previous models: the attachment point theory and the engineered roughness index (eri) model. the new surface energetic attachment (sea) model is based on both the properties of the cell-material interface and the size and configuration of the topography relative to the organism. we have used monte carlo simulation to examine the sea model's ability to predict relative attachment of the gr ... | 2013 | 24044383 |
| adhesion of marine fouling organisms on hydrophilic and amphiphilic polysaccharides. | polysaccharides are a promising material for nonfouling surfaces because their chemical composition makes them highly hydrophilic and able to form water-storing hydrogels. here we investigated the nonfouling properties of hyaluronic acid (ha) and chondroitin sulfate (cs) against marine fouling organisms. additionally, the free carboxyl groups of ha and cs were postmodified with the hydrophobic trifluoroethylamine (tfea) to block free carboxyl groups and render the surfaces amphiphilic. all coati ... | 2013 | 23425225 |
| identification and characterization of an extracellular alkaline phosphatase in the marine diatom phaeodactylum tricornutum. | in phosphorus-deficient conditions, phaeodactylum tricornutum releases an alkaline phosphatase (ptapase) to the medium that is readily detectable by activity staining. nucleic acid and amino acid sequence of this alkaline phosphatase (apase) was identified by performing proteomic analysis and database searches. sequence alignment suggests that ptapase belongs to the phoa family, and it possesses key residues at the escherichia coli phoa active site. quantitative pcr results indicate that the ind ... | 2013 | 23358911 |
| effect of bacterial biofilms formed on fouling-release coatings from natural seawater and cobetia marina, on the adhesion of two marine algae. | previous studies have shown that bacterial biofilms formed from natural seawater (nsw) enhance the settlement of spores of the green alga ulva linza, while single-species biofilms may enhance or reduce settlement, or have no effect at all. however, the effect of biofilms on the adhesion strength of algae, and how that may be influenced by coating/surface properties, is not known. in this study, the effect of biofilms formed from natural seawater and the marine bacterium cobetia marina, on the se ... | 2012 | 23004017 |
| quantifying the effect of covalently immobilized enzymes on biofilm formation by atomic force microscopy-based single-cell force spectroscopy. | a novel atomic force microscopy-based single-cell force spectroscopy assay to quantify the adhesion of bacterial cells to surfaces was developed. the assay was applied to quantify the effect of two biofilm-degrading enzymes, the protease subtilisin a and glycoside hydrolase cellulase, on the attachment of the biofilm-forming bacterial strain cobetia marina. insights on the mechanism of the initial adhesion and on the nature of the adhesion-mediating molecules were gained. the assay can be easily ... | 2012 | 22829309 |
| microfluidic assay to quantify the adhesion of marine bacteria. | for both, environmental and medical applications, the quantification of bacterial adhesion is of major importance to understand and support the development of new materials. for marine applications, the demand is driven by the quest for improved fouling-release coatings. to determine the attachment strength of bacteria to coatings, a microfluidic adhesion assay has been developed which allows probing at which critical wall shear stress bacteria are removed from the surface. besides the experimen ... | 2012 | 22589069 |
| description of cobetia amphilecti sp. nov., cobetia litoralis sp. nov. and cobetia pacifica sp. nov., classification of halomonas halodurans as a later heterotypic synonym of cobetia marina and emended descriptions of the genus cobetia and cobetia marina. | a group of five gram-negative, aerobic, halotolerant, non-pigmented bacteria isolated from shallow sediment samples and invertebrate specimens collected from the gulf of alaska and the sea of japan was subjected to taxonomic study. on the basis of 16s rrna gene sequence analysis, the novel isolates were affiliated to the genus cobetia, sharing the highest sequence similarity of 99.3-99.9 % with cobetia marina dsm 4741(t). dna-dna hybridization experiments between and among the novel strains and ... | 2013 | 22427450 |
| antibiofilm activity of cobetia marina filtrate upon staphylococcus epidermidis catheter-related isolates. | we report the antibiofilm activity by the sponge-associated bacterium cobetia marina upon staphylococcus epidermidis clinical isolates obtained from central venous catheters. antibiofilm activity/antimicrobial susceptibility correlation might predict the action of the metabolite(s) upon staphylococcus epidermidis in the clinic, making it a possible adjuvant in therapies against biofilm-associated infections. | 2011 | 24031760 |
| inhibition of larval barnacle attachment to bacterial films: an investigation of physical properties. | the effects of films of two strains of a marine bacterium, deleya marina (atcc 25374 and 27129) on the attachment response of cypris larvae of the balanomorph barnacle, balanus amphitrite, were examined in the laboratory. tests showed that the cell-surface hydrophobicities of the two bacteria in suspension were different. in contrast, films derived from these cells were both highly wettable (i.e., displayed high surface free energy). assays (22 hours) compared permanent attachment of larval barn ... | 1992 | 24192832 |