| complete oxidation of toluene under strictly anoxic conditions by a new sulfate-reducing bacterium. | a toluene-degrading sulfate-reducing bacterium, strain tol2, was isolated from marine sediment under strictly anoxic conditions. toluene was toxic if applied directly to the medium at concentrations higher than 0.5 mm. to provide toluene continuously at a nontoxic concentration, it was supplied in an inert hydrophobic carrier phase. the isolate had oval, sometimes motile cells (1.2 to 1.4 by 1.2 to 2.0 microns). the doubling time was 27 h. toluene was completely oxidized to co2, as demonstrated ... | 1993 | 7686000 |
| conversion studies with substrate analogues of toluene in a sulfate-reducing bacterium, strain tol2. | anaerobic toluene oxidation by the sulfate-reducing bacterium, strain tol2 (proposed name desulfobacula toluolica) was specifically inhibited by benzyl alcohol when added at concentrations around 500 microm. benzyl alcohol added at lower, non-inhibitory concentrations (around 5 microm) was not oxidized by active cells pregrown on toluene, indicating that the alcohol is not a free intermediate of toluene metabolism in the sulfate reducer. conversion of p-xylene in toluene-metabolizing cells to p- ... | 1995 | 8588748 |
| demethylation of dimethylsulfoniopropionate to 3-s-methylmercaptopropionate by marine sulfate-reducing bacteria. | the initial step in the anaerobic degradation of the algal osmolyte dimethylsulfoniopropionate (dmsp) in anoxic marine sediments involves either a cleavage to dimethylsulfide and acrylate or a demethylation to 3-s-methylmercaptopropionate. thus far, only one anaerobic bacterial strain has been shown to carry out the demethylation, namely, desulfobacterium sp. strain pm4. the aims of the present work were to study how common this property is among certain groups of anaerobic bacteria and to obtai ... | 1996 | 8899985 |
| anaerobic degradation and transformation of p-toluidine by the sulfate-reducing bacterium desulfobacula toluolica. | the ability of the strictly anaerobic sulfate-reducing bacterium desulfobacula toluolica (strain tol2) to cometabolically degrade p-toluidine (p-methylaniline) while using toluene as the primary source of carbon and energy has been studied. this organism has been shown to modify and degrade toluidine in dense cell suspensions when no other source of carbon and energy is added. the metabolism led to the formation of a variety of metabolites. from these metabolites a biphenyl-like compound as well ... | 1998 | 9688816 |
| reclassification of desulfobacterium phenolicum as desulfobacula phenolica comb. nov. and description of strain saxt as desulfotignum balticum gen. nov., sp. nov. | a mesophilic, sulfate-reducing bacterium (strain saxt) was isolated from marine coastal sediment in the baltic sea and originally described as a 'desulfoarculus' sp. it used a large variety of substrates, ranging from simple organic compounds and fatty acids to aromatic compounds as electron donors. autotrophic growth was possible with h2, co2 and formate in the presence of sulfate. sulfate, thiosulfate and sulfite were used as electron acceptors. sulfur and nitrate were not reduced. fermentativ ... | 2001 | 11211256 |
| multiple lateral transfers of dissimilatory sulfite reductase genes between major lineages of sulfate-reducing prokaryotes. | a large fragment of the dissimilatory sulfite reductase genes (dsrab) was pcr amplified and fully sequenced from 30 reference strains representing all recognized lineages of sulfate-reducing bacteria. in addition, the sequence of the dsrab gene homologs of the sulfite reducer desulfitobacterium dehalogenans was determined. in contrast to previous reports, comparative analysis of all available dsrab sequences produced a tree topology partially inconsistent with the corresponding 16s rrna phylogen ... | 2001 | 11567003 |
| diversity and abundance of bacteria in an underground oil-storage cavity. | microorganisms inhabiting subterranean oil fields have recently attracted much attention. since intact groundwater can easily be obtained from the bottom of underground oil-storage cavities without contamination by surface water, studies on such oil-storage cavities are expected to provide valuable information to understand microbial ecology of subterranean oil fields. | 2002 | 12197947 |
| lateral gene transfer of dissimilatory (bi)sulfite reductase revisited. | in contrast to previous findings, we demonstrate that the dissimilatory (bi)sulfite reductase genes (dsrab) of desulfobacula toluolica were vertically inherited. furthermore, desulfobacterium anilini and strain mxys1 were identified, by dsrab sequencing of 17 reference strains, as members of the donor lineage for those gram-positive desulfotomaculum species which laterally acquired dsrab. | 2005 | 15743970 |
| identity and abundance of active sulfate-reducing bacteria in deep tidal flat sediments determined by directed cultivation and card-fish analysis. | the identity and abundance of potentially active sulfate-reducing bacteria (srb) in several metre deep sediments of a tidal sand flat in the german wadden sea were assessed by directed cultivation and cultivation-independent card-fish analysis (catalysed reporter deposition fluorescence in situ hybridization). presumably abundant srb from different sediment layers between 0.5 and 4 m depth were selectively enriched in up to million-fold diluted cultures supplemented with lactate, acetate or hydr ... | 2008 | 18627412 |
| sulfur and oxygen isotope fractionation during benzene, toluene, ethyl benzene, and xylene degradation by sulfate-reducing bacteria. | we examined the oxygen and sulfur isotope fractionation of sulfate during anaerobic degradation of toluene by sulfate-reducing bacteria in culture experiments with desulfobacula toluolica as a type strain and with an enrichment culture zz5-7 obtained from a benzene, toluene, ethylbenzene, and xylene (btex)-contaminated aquifer. sulfur isotope fractionation can show considerable variation upon sulfate reduction and may react extremely sensitively to changes in environmental conditions. in contras ... | 2006 | 16830556 |
| population profiles of a stable, commensalistic bacterial culture grown with toluene under sulphate-reducing conditions. | most bacteria present in nature are not culturable in pure culture by means of classic cultivation methods (pace nr, 1997, science 276:734-740; amann ri et al., 1995, microbiol rev 59:143-169.). however, it was recently shown that most aerobic heterotrophic bacteria could grow only on artificial media when other micro-organisms are present (kaeberlein t et al., 2002, science 296:1127-1129). because the sulphate reducer desulfobacula toluolica dsm 7467 and a bacterium (strain mv1) identified as c ... | 2005 | 16003722 |
| molecular characterization of sulfate-reducing bacteria in the guaymas basin. | the guaymas basin (gulf of california) is a hydrothermal vent site where thermal alteration of deposited planktonic and terrestrial organic matter forms petroliferous material which supports diverse sulfate-reducing bacteria. we explored the phylogenetic and functional diversity of the sulfate-reducing bacteria by characterizing pcr-amplified dissimilatory sulfite reductase (dsrab) and 16s rrna genes from the upper 4 cm of the guaymas sediment. the dsrab sequences revealed that there was a major ... | 2003 | 12732547 |