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environmental fate and microbial degradation of aminopolycarboxylic acids.aminopolycarboxylic acids (apcas) have the ability to form stable, water-soluble complexes with di- and trivalent metal ions. for that reason, synthetic apcas are used in a broad range of domestic products and industrial applications to control solubility and precipitation of metal ions. because most of these applications are water-based, apcas are disposed of in wastewater and reach thus sewage treatment plants and the environment, where they undergo abiotic and/or biotic degradation processes. ...200111152941
microvirga subterranea gen. nov., sp. nov., a moderate thermophile from a deep subsurface australian thermal aquifer.a strictly aerobic bacterium, strain fail4t, was isolated from free-flowing geothermal waters of a bore (bore register no. 3768) tapping the great artesian basin of australia. the non-sporulating, gram-negative cells of strain fail4t produced light-pink colonies, were rod-shaped (1 x 1.5-4 microm) and were motile by a single polar flagellum. strain fail4t grew optimally at 41 degrees c at a ph of 7.0 and had an absolute requirement for yeast extract. the strain grew on casein hydrolysate, trypto ...200312710604
the variable part of the dnak gene as an alternative marker for phylogenetic studies of rhizobia and related alpha proteobacteria.dnak is the 70 kda chaperone that prevents protein aggregation and supports the refolding of damaged proteins. due to sequence conservation and its ubiquity this chaperone has been widely used in phylogenetic studies. in this study, we applied the less conserved part that encodes the so-called alpha-subdomain of the substrate-binding domain of dnak for phylogenetic analysis of rhizobia and related non-symbiotic alpha-proteobacteria. a single 330 bp dna fragment was routinely amplified from dna t ...200314666974
biodegradation of metal-complexing aminopolycarboxylic acids.aminopolycarboxylic acids (apcas) are used in a broad range of household products and industrial applications to control the solubility and precipitation of metal ions. the two most widely used apcas are ethylenediaminetetraacetate (edta) and nitrilotriacetate (nta). because their application is mostly water-based apcas are disposed off in wastewater from where they reach the environment if not sufficiently well eliminated. a number of nta and edta-degrading bacterial strains have been isolated. ...200116233066
sneathiella chinensis gen. nov., sp. nov., a novel marine alphaproteobacterium isolated from coastal sediment in qingdao, china.the taxonomic position of strain lmg 23452(t), which was isolated from coastal sediment from an aquaculture site near qingdao, china, in 2000, was determined. strain lmg 23452(t) comprised gram-negative, non-spore-forming, motile rods and was found to be a halotolerant, aerobic, chemoheterotroph that produces catalase and oxidase. comparative 16s rrna gene sequence analysis revealed that strain lmg 23452(t) shared approximately 89 % sequence similarity with members of the genera devosia, hyphomo ...200717220452
novel alphaproteobacterial root nodule symbiont associated with lupinus texensis.phylogenetic analysis of rrna gene, reca, noda, nifd, and nifh sequences suggested that nitrogen-fixing symbionts from two populations of lupinus texensis acquired the capacity for nodule symbiosis separately from other rhizobia in the alphaproteobacteria. their closest 16s rrna relatives were the nonsymbiotic taxa chelatococcus, bosea, and balneomonas.200717616612
protein oxidation: key to bacterial desiccation resistance?for extremely ionizing radiation-resistant bacteria, survival has been attributed to protection of proteins from oxidative damage during irradiation, with the result that repair systems survive and function with far greater efficiency during recovery than in sensitive bacteria. here we examined the relationship between survival of dry-climate soil bacteria and the level of cellular protein oxidation induced by desiccation. bacteria were isolated from surface soils of the shrub-steppe of the us d ...200818273068
chelatococcus daeguensis sp. nov., isolated from wastewater of a textile dye works, and emended description of the genus chelatococcus.a gram-negative, non-spore-forming, rod-shaped bacterial strain, k106(t), was isolated from wastewater collected from a textile dye works in korea. strain k106(t) grew optimally at ph 7.0-7.5 and 30-37 degrees c in the presence of 0-1.0% (w/v) nacl. a phylogenetic tree based on 16s rrna gene sequences showed that strain k106(t) joined the type strain of chelatococcus asaccharovorans with a bootstrap resampling value of 99.9%. the predominant ubiquinone of strain k106(t) was q-10. the fatty acid ...200818768633
salinarimonas rosea gen. nov., sp. nov., a new member of the alpha-2 subgroup of the proteobacteria.a gram-negative, rod-shaped, facultatively anaerobic, halotolerant bacterial strain, designated yim yd3(t), was isolated from a salt mine in yunnan, south-west china. the taxonomy of strain yim yd3(t) was investigated by a polyphasic approach. strain yim yd3(t) was motile, formed pink colonies and was positive for catalase and oxidase activities. q-10 was the predominant respiratory ubiquinone. the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolami ...201019648350
chelatococcus sambhunathii sp. nov., a moderately thermophilic alphaproteobacterium isolated from hot spring sediment.a moderately thermophilic bacterial strain (ht4(t)) isolated from a hot spring sediment was characterized phenotypically and phylogenetically. cells were gram-negative, aerobic, non-sporulating, rod-shaped and motile by means of a single polar flagellum. both oxidase and catalase activities were positive. heterotrophic growth was observed at ph 6.0-8.5 and at 20-50 degrees c; optimum growth occurred at ph 7.5-8.0 and 37-42 degrees c. the major cellular fatty acids were c( 14 : 0) 3-oh, c(18 : 0) ...201019661510
amplified ribosomal dna restriction analysis of free-living bacteria present in the headbox of a canadian paper machine.the headbox water is the main source of bacterial contamination of paper machines. identification of these bacterial contaminants could be an asset in developing specific control methods. an amplified ribosomal dna restriction analysis (ardra) was carried out to characterize the bacterial communities associated with the headbox water of a paper machine in a canadian mill in february and july 2006. eight bacterial genera were identified as the main colonizers present in the headbox water. the gen ...200919767853
camelimonas lactis gen. nov., sp. nov., isolated from the milk of camels.three strains of gram-negative, rod-shaped, non-spore-forming bacteria (m 2040(t), m 1973 and m 1878-sk2), isolated from milk of camels at a camel-milk production farm in the united arab emirates, were investigated for their taxonomic allocation. on the basis of 16s rrna gene sequence similarities, all three strains were shown to belong to the alphaproteobacteria and were most closely related to chelatococcus asaccharovorans and chelatococcus daeguensis (95.1 and 95.2 % sequence similarity to th ...201019933583
isolation and characterization of new poly(3hb)-accumulating star-shaped cell-aggregates-forming thermophilic bacteria.this study aimed at isolating thermophilic bacteria that utilize cheap carbon substrates for the economically feasible production of poly(3-hydroxybutyrate), poly(3hb), at elevated temperatures.201020584099
high-cell-density cyclic fed-batch fermentation of a poly(3-hydroxybutyrate)-accumulating thermophile, chelatococcus sp. strain mw10.different fermentation strategies were employed for the cultivation of a new poly(3-hydroxybutyrate)-accumulating thermophilic bacterium, chelatococcus sp. strain mw10, with the aim of achieving high-cell-density (hcd) growth and high poly(3-hydroxybutyrate) [poly(3hb)] productivity. enhanced cultivation was achieved by a cyclic fed-batch fermentation (cfbf) technique (42-liter scale). maximal poly(3hb) productivity was obtained during the second cycle [16.8 ± 4.2 g poly(3hb)/liter]. at the end ...201020889784
[identification and denitrification characteristics of a thermophilic aerobic denitrifier].a bacterial strain tad1 with high nitrogen removal efficiency was isolated from biofilm of the biotrickling filter of a coal-fired power plant by thermophilic domestication. this bacterium was gram negative, short rod, with the size of (0.67-0.89) microm x (1.03-1.41) microm. it was identified as chelatococcus sp. according to its physiological properties and the analysis of its 16s rdna gene. studied on its function of aerobic denitrification at the temperature of 50 degrees c, the results show ...201121404696
Microbial removal of NO(X) at high temperature by a novel aerobic strain Chelatococcus daeguensis TAD1 in a biotrickling filter.The removal of NO(X) at high temperature by Chelatococcus daeguensis TAD1 in a biotrickling filter was studied. Media components of the recycling liquid were screened using Plackett-Burman design and then were optimized using response surface methodology, which enhanced the efficiency of nitrate removal by TAD1. The optimal medium was used to perform long-term experiments of NO(X) removal in a biotrickling filter under high concentrations of O(2) and NO in simulated flue gas. Results showed that ...201122209326
Evaluation of the coal-degrading ability of Rhizobium and chelatococcus strains isolated from the formation water of an Indian coal bed.The rise in global energy demand has prompted researches on developing strategies for transforming coal into a cleaner fuel. This requires isolation of microbes with the capability to degrade complex coal into simpler substrates to support methanogenesis in the coal beds. In this study, aerobic bacteria were isolated from an Indian coal bed that can solubilize and utilize coal as the sole source of carbon. The six bacterial isolates capable of growing on coal agar medium were identified on the b ...201122127119
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