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polyphasic taxonomic study of the emended genus arcobacter with arcobacter butzleri comb. nov. and arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens.the relationships of 77 aerotolerant arcobacter strains that were originally identified as campylobacter cryaerophila (now arcobacter cryaerophilus [p. vandamme, e. falsen, r. rossau, b. hoste, p. segers, r. tytgat, and j. de ley, int. j. syst. bacteriol. 41:88-103, 1991]) and 6 reference strains belonging to the taxa arcobacter nitrofigilis, arcobacter cryaerophilus, and "campylobacter butzleri" were studied by using a polyphasic approach, in which we performed dna-rrna hybridizations, dna-dna ...19921503968
arcobacter-specific and arcobacter butzleri-specific 16s rrna-based dna probes.the genus arcobacter encompasses gram-negative, aerotolerant, spiral-shaped bacteria formerly designated campylobacter cryaerophila. two genus-specific 16s rrna-based oligonucleotide dna probes (23-mer and 27-mer) were developed. the probes hybridized with strains of arcobacter butzleri (n = 58), arcobacter cryaerophilus (n = 19), and arcobacter skirrowii (n = 17). the probes did not cross-react with any of the reference strains of campylobacter, helicobacter, including "flexispira rappini," or ...19957545177
infection of cesarean-derived colostrum-deprived 1-day-old piglets with arcobacter butzleri, arcobacter cryaerophilus, and arcobacter skirrowii.neonatal piglets have been used as models to study human campylobacteriosis and helicobacteriosis. the purpose of this study was to determine the relative pathogenicities, on the basis of the duration of fecal shedding and colonization of tissues, of three arcobacter species in 1-day-old cesarean-derived colostrum-deprived piglets. two experiments were conducted. in experiment 1, two piglets each were infected per os with either arcobacter butzleri atcc 49616, arcobacter cryaerophilus 1b atcc 43 ...19968675340
identification of arcobacter isolates by pcr.a polymerase chain reaction (pcr) assay was developed for the identification of the three species of arcobacter which have been recovered from clinically ill or healthy humans and/or livestock, namely arcobacter butzleri, arcobacter skirrowii and arcobacter cryaerophilus. the assay utilizes primers targeted to the genes encoding 16s rrna of arcobacter spp. the assay reduces the amount of time required to positively identify strains of arcobacter.19968987697
development of a multiplex pcr assay for the simultaneous detection and identification of arcobacter butzleri, arcobacter cryaerophilus and arcobacter skirrowii.a multiplex pcr assay with five primers targeting the 16s and 23s rrna genes was developed for the simultaneous detection and identification of arcobacter butzleri, arcobacter cryaerophilus and arcobacter skirrowii. the selected primers amplify a 257-bp fragment from a. cryaerophilus, a 401-bp fragment from a. butzleri and a 641-bp fragment from a. skirrowii. no pcr product was generated for closely related bacteria including campylobacter and helicobacter species. the assay was useful to identi ...200011094284
susceptibility of arcobacter butzleri, arcobacter cryaerophilus, and arcobacter skirrowii to antimicrobial agents used in selective media.several antimicrobial agents used in selective media for the isolation of arcobacter were found to be inhibitory to strains belonging to this genus. all three species tested were susceptible to colistin and rifampin at concentrations used in selective media. arcobacter skirrowii was the most susceptible species. 5-fluorouracil, novobiocin, trimethoprim, and teicoplanin or vancomycin were found to be without any inhibitory effect on the strains tested at concentrations described for the isolation ...200111283110
assessment of the genetic diversity among arcobacters isolated from poultry products by using two pcr-based typing methods.in this study, enterobacterial repetitive intergenic consensus pcr (eric-pcr) and randomly amplified polymorphic dna pcr (rapd-pcr) were optimized for characterization of arcobacter butzleri, arcobacter cryaerophilus, and arcobacter skirrowii. in addition, a simple and rapid dna extraction method was tested for use in both typing procedures. both methods had satisfactory typeability and discriminatory power, but the fingerprints generated with eric-pcr were more reproducible and complex than tho ...200211976086
one-step polymerase chain reaction-based typing of arcobacter species.a species-specific pcr assay was developed for the identification of the arcobacter species, arcobacter butzleri, arcobacter cryaerophilus 1a and 1b, and arcobacter skirrowii. the primers, which amplify the most variable areas of the 23s rrna gene, were designed to perform species-specific identification by one-step pcr. the dna sequence of the region from a. cryaerophilus 1b was determined, and the specific pimer for the species was designed. by using one-step pcr containing the mixed primers n ...200312457591
detection of arcobacter spp. in the coastal environment of the mediterranean sea.the occurrence of arcobacter spp. was studied in seawater and plankton samples collected from the straits of messina, italy, during an annual period of observation by using cultural and molecular techniques. a pcr assay with three pairs of primers targeting the 16s and 23s rrna genes was used for detection and identification of arcobacter butzleri, arcobacter cryaerophilus, and arcobacter skirrowii in cultures and environmental samples. only one of the arcobacter species, a. butzleri, was isolat ...200415006743
isolation of arcobacter skirrowii from a patient with chronic diarrhea. 200415071069
a comparison of three methods for the isolation of arcobacter spp. from retail raw poultry in northern ireland.recent evidence suggests that arcobacters, especially arcobacter butzleri, are potential foodborne pathogens, but standardized detection methods have yet to be established. a study was undertaken to determine which of three isolation methods was the most effective for the isolation of arcobacter spp. from fresh raw poultry. methods 1 was microaerobic and involved a membrane filtration step followed by plating onto blood agar. method 2 was also microaerobic and involved enrichment and plating med ...200415083734
arcobacter cibarius sp. nov., isolated from broiler carcasses.twenty gram-negative, rod-shaped, slightly curved, non-spore-forming bacteria that gave a negative result in arcobacter species-specific pcr tests but that yielded an amplicon in an arcobacter genus-specific pcr test were isolated from 13 unrelated broiler carcasses. numerical analysis of the profiles obtained by sds-page of whole-cell proteins clustered all isolates in a single group distinct from the other arcobacter species. dna-dna hybridization among four representative strains exhibited dn ...200515774649
detection and diversity of various arcobacter species in danish poultry.the prevalence and diversity of different arcobacter spp. in various poultry species in denmark were investigated using cultural and multiplex pcr methods. a pool of three fresh droppings obtained at the production site from 70 broiler chicken flocks aged 4-5 weeks was examined. in addition, pools of 10 cloacal swabs taken at the abattoir prior to stunning from each of 15, and 37 duck and turkey flocks, respectively, were analyzed. thirty fresh broiler chicken carcasses and 29 cloacal swabs from ...200616516995
arcobacter, what is known and unknown about a potential foodborne zoonotic agent!since the introduction of the genus arcobacter in 1991, the association of arcobacter butzleri, arcobacter cryaerophilus and arcobacter skirrowii with humans and animals has been clearly established. these bacteria have been detected world wide in products of animal origin and in healthy animals as well as in surface water. a fourth species arcobacter cibarius was recently discovered on chicken carcasses. although evidence was found for the connection of arcobacter spp. with human and animal ill ...200616621345
prevalence of arcobacter spp. in raw milk and retail raw meats in northern ireland.a 1-year study was undertaken to determine the prevalence of arcobacter spp. in raw milk and retail raw meats on sale in northern ireland. retail raw poultry samples (n = 94), pork samples (n = 101), and beef samples (n = 108) were obtained from supermarkets in northern ireland, and raw milk samples (n = 101) were kindly provided by the milk research laboratory, department of agriculture and rural development, belfast, northern ireland. presumptive arcobacters were identified by previously descr ...200616924929
development of a multiplex and real time pcr assay for the specific detection of arcobacter butzleri and arcobacter cryaerophilus.a new multiplex pcr and two specific taqman assays were developed to target the emerging pathogens a. butzleri and a. cryaerophilus. the assays also included an internal control to verify the presence of bacterial target dna and amplification integrity. the multiplex assay used a published primer set (cry1 and cry2) for detecting a. cryaerophilus dna (houf, k., tutenel, a., de zutter, l., van hoof, j. and vandamme, p., 2000. development of a multiplex pcr assay for the simultaneous detection and ...200717055091
direct detection and identification of arcobacter species by multiplex pcr in chicken and wastewater samples from spain.twenty-two chicken livers, 10 chicken carcasses, and 15 wastewater samples were processed and analyzed for arcobacter by pcr and traditional culture methods. samples were enriched for 24 and 48 h, incubated at 30 degrees c under aerobic conditions, and streaked on blood selective media. to determine the best isolation conditions, 20 samples also were processed under microaerophilic conditions at 37 degrees c. simple and multiplex pcr assays were used directly with enrichment broths and isolated ...200717340867
nucleotide sequence of the gyra gene of arcobacter species and characterization of human ciprofloxacin-resistant clinical isolates.the nucleotide sequence of the gyra gene of arcobacter butzleri, arcobacter cryaerophilus, arcobacter cibarius, and arcobacter skirrowii was determined. the deduced gyra proteins are closely related to those of wolinella succinogenes and helicobacter pullorum, whereas those of campylobacter species showed less sequence identity. the phylogenetic analysis of gyra sequences provides a result similar to 16s rrna gene sequence phylogenetic analysis and allows the discrimination among a. butzleri spe ...200717378897
prevalence and distribution of arcobacter species in various sources in turkey and molecular analysis of isolated strains by eric-pcr.to determine the prevalence of arcobacter in various food, animal and water sources in turkey and to subtype the isolated strains using enterobacterial repetitive intergenic consensus (eric)-pcr.200717584450
presence of arcobacter spp. in environmental waters correlates with high levels of fecal pollution.we investigated the presence of arcobacter spp. in 205 water samples of freshwater, seawater and sewage in spain. these bacteria were present in 55.1% of the samples (113/205) and were significantly associated for the first time with bacterial indicators of fecal pollution. the dominant species in the positive samples was arcobacter butzleri (94%) followed by arcobacter cryaerophilus (30%) and arcobacter skirrowii (1.8%).200818215159
inhibition of arcobacter butzleri, arcobacter cryaerophilus, and arcobacter skirrowii by plant oil aromatics.the inhibitory effect of some plant oil aromatics against three strains of arcobacter butzleri, two strains of arcobacter cryaerophilus, and one strain of arcobacter skirrowii was evaluated. when mics were determined using the broth macrodilution method, cinnamaldehyde was most inhibitory followed by thymol, carvacrol, caffeic acid, tannic acid, and eugenol (p < 0.001). sublethal concentrations of the three most potent plant oil aromatics also were examined. overall, cinnamaldehyde was the most ...200818236678
development of a strain-specific molecular method for quantitating individual campylobacter strains in mixed populations.the identification of sites resulting in cross-contamination of poultry flocks in the abattoir and determination of the survival and persistence of campylobacters at these sites are essential for the development of intervention strategies aimed at reducing the microbial burden on poultry at retail. a novel molecule-based method, using strain- and genus-specific oligonucleotide probes, was developed to detect and enumerate specific campylobacter strains in mixed populations. strain-specific oligo ...200818281428
the presence of arcobacter species in breeding hens and eggs from these hens.the presence of arcobacter spp. in 2 breeding hen flocks was determined by examination of the intestinal tract, oviduct magnum mucosa, and ovarian follicles of slaughtered chicken. the bacteria were detected by pcr and cultural isolation in 34 out of 40 intestinal tracts from one flock (a) and 6 out of 30 from the other (b). the strains were arcobacter butzleri, arcobacter cryaerophilus, and arcobacter skirrowii. from flock a, arcobacters were recovered from 6 out of 40 oviduct magnum mucosa sam ...200818931194
pet cats as carriers of arcobacter spp. in southern italy.to evaluate the presence of arcobacter spp. in different biological samples from domestic cats in southern italy by using a species-specific pcr assay and thus to elucidate their potential significance as sources of human infection.200919226387
prevalence of arcobacter in meat and shellfish.arcobacter is considered an emergent foodborne and waterborne enteropathogen. however, its prevalence in foods of animal origin is only partially known, because most studies have been concentrated on poultry, pork, and beef, and methods applied do not allow identification of all currently accepted arcobacter species. we investigated the prevalence of arcobacter in 203 food samples, 119 samples of seven different types of meats and 84 samples of four types of shellfish. isolates were identified i ...200919517742
arcobacter thereius sp. nov., isolated from pigs and ducks.during a danish study on the prevalence of campylobacteria in pig abortions and food of animal origin, eight gram-negative, slightly curved, rod-shaped, non-spore-forming bacteria were clustered by using amplified fragment length polymorphism analysis in a distinct phenon within the genus arcobacter. in the present study, numerical analysis of whole-cell protein profiles also showed that all isolates clustered in a single group distinct from other recognized arcobacter species. dna-dna hybridiza ...200919622651
specific pcr detection of arcobacter butzleri, arcobacter cryaerophilus, arcobacter skirrowii, and arcobacter cibarius in chicken meat.an enrichment pcr assay using species-specific primers was developed for the detection of arcobacter butzleri, arcobacter cryaerophilus, arcobacter skirrowii, and arcobacter cibarius in chicken meat. primers for a. cryaerophilus, a. skirrowii, and a. cibarius were designed based on the gyra gene to amplify nucleic acid fragments of 212, 257, and 145 bp, respectively. the a. butzleri-specific primers were designed flanking a 203-bp dna fragment in the 16s rrna gene. the specificity of the four pr ...200919681276
rapid and accurate detection of arcobacter contamination in commercial chicken products and wastewater samples by real-time polymerase chain reaction.an sybr green real-time polymerase chain reaction (pcr) assay was developed for arcobacter detection in food and wastewater samples. the assay was applied to 36 chicken and 33 wastewater samples, and the results were compared with those obtained for conventional pcr, multiplex pcr, and culture isolation. isolates were identified by multiplex pcr and restriction fragment length polymorphism analysis of pcr-amplified dna fragment, and typed by randomly amplified polymorphic dna. arcobacter sp. was ...201019899959
occurrence and diversity of arcobacter spp. along the llobregat river catchment, at sewage effluents and in a drinking water treatment plant.the presence of arcobacter species in faecally contaminated environmental waters has previously been studied. however, the ability to eliminate arcobacter during the water treatment processes that produce drinking water has been little studied. we have investigated the prevalence and diversity of arcobacter spp. throughout the year at 12 sampling points in the llobregat river catchment (catalonia, spain) including 3 sites at a drinking water treatment plant. positive samples for arcobacter spp., ...201020427071
inhibitory effect of organic acids on arcobacters in culture and their use for control of arcobacter butzleri on chicken skin.the inhibitory effects of 17 organic acids (c₂-c₁₆ fatty acids, sorbic, benzoic, phenylacetic, fumaric, succinic, lactic, malic and citric) on arcobacter butzleri, arcobacter cryaerophilus and arcobacter skirrowii were investigated by determining their ic₅₀ values, defined as the concentration of acid at which the target dna sequence was expressed at 50% of the positive control level in cultures incubated at 30°c for 24 h. dna was analysed by real-time pcr. the arcobacter strains tested were inh ...201021084127
inhibitory effects of some plant essential oils against arcobacter butzleri and potential for rosemary oil as a natural food preservative.we investigated the inhibitory activity of commercially marketed essential oils of mint, rosemary, orange, sage, cinnamon, bay, clove, and cumin against arcobacter butzleri and arcobacter skirrowii and the effects of the essential oil of rosemary against a. butzleri in a cooked minced beef system. using the disc diffusion method to determine the inhibitory activities of these plant essential oils against strains of arcobacter, we found that those of rosemary, bay, cinnamon, and clove had strong ...201021142947
prevalence of arcobacter species in drinking water, spring water, and raw milk as determined by multiplex pcr.the aim of this study was to investigate the incidence of arcobacter species in water sources and raw milk from healthy animals in kayseri, turkey. a total of 175 samples of drinking water (n = 100), spring water (n = 25), and raw milk (n = 50) were examined. arcobacter species were isolated using the membrane filtration technique. overall, 7 (4%) of the 175 samples yielded arcobacter spp.: 3 (3%) drinking water samples, 1 (4%) spring water sample, and 3 (6%) raw milk samples. two species of arc ...201021219725
occurrence of putative virulence genes in arcobacter species isolated from humans and animals.interest for arcobacters in veterinary and human public health has increased since the first report of the isolation of arcobacters from food of animal origin. since then, studies worldwide have reported the occurrence of arcobacters on food and in food-production animals and have highlighted the possible transmission of especially a. butzleri to the human population. in humans, arcobacters are associated with enteritis and septicemia. to assess their clinical relevance for humans and animals, e ...201122170914
recovery of arcobacter spp. from nonlivestock species.the genus arcobacter encompasses campylobacter-like organisms that grow in air at 25 degrees c. arcobacter has been detected or isolated from clinically healthy livestock as well as aborted fetuses and has been presumptively identified as either campylobacter or leptospira, based on its growth in selective semisolid media. because reports from nonlivestock species are limited, this study examined nine presumptive isolates of arcobacter spp. from an alpaca (vicugna pacos), black rhinoceros (dicer ...201122950328
comparison of conventional pcr, multiplex pcr, and loop-mediated isothermal amplification assays for rapid detection of arcobacter species.this study aimed to develop a loop-mediated isothermal amplification (lamp) method for the rapid detection of arcobacter species. specific primers targeting the 23s ribosomal rna gene were used to detect arcobacter butzleri, arcobacter cryaerophilus, and arcobacter skirrowii. the specificity of the lamp primer set was assessed using dna samples from a panel of arcobacter and campylobacter species, and the sensitivity was determined using serial dilutions of arcobacter species cultures. lamp show ...201424478488
occurrence, diversity, and host association of intestinal campylobacter, arcobacter, and helicobacter in reptiles.campylobacter, arcobacter, and helicobacter species have been isolated from many vertebrate hosts, including birds, mammals, and reptiles. multiple studies have focused on the prevalence of these epsilonproteobacteria genera in avian and mammalian species. however, little focus has been given to the presence within reptiles, and their potential zoonotic and pathogenic roles. in this study, occurrence, diversity, and host association of intestinal epsilonproteobacteria were determined for a large ...201424988130
arcobacter butzleri: first isolation report from chicken carcasses in costa rica.arcobacter butzleri isolation from chicken carcasses in costa rica is reported for the first time. the isolated strains (p and r) were presumptively identified by their phenotypic characteristics. definitive identification was made using a multiplex pcr assay for the simultaneous detection and identification of arcobacter butzleri, arcobacter cryaerophilus and arcobacter skirrowii. these first isolations indicate the necessity of further investigation about the prevalence, distribution, ecology ...201124031682
the use of two culturing methods in parallel reveals a high prevalence and diversity of arcobacter spp. in a wastewater treatment plant.the genus arcobacter includes species considered emerging food and waterborne pathogens. despite arcobacter has been linked to the presence of faecal pollution, few studies have investigated its prevalence in wastewater, and the only isolated species were arcobacter butzleri and arcobacter cryaerophilus. this study aimed to establish the prevalence of arcobacter spp. at a wwtp using in parallel two culturing methods (direct plating and culturing after enrichment) and a direct detection by m-pcr. ...201627981053
development and evaluation of multiplex pcr assays for rapid detection of virulence-associated genes in arcobacter species.as the pathogenicity of arcobacter species might be associated with various virulence factors, this study was aimed to develop and optimize three single-tube multiplex pcr (mpcr) assays that can efficiently detect multiple virulence-associated genes (vags) in arcobacter spp. including the arcobacter butzleri, arcobacter cryaerophilus and arcobacter skirrowii, respectively. the recognized target virulence factors used in the study were fibronectin binding protein (cj1349), filamentous hemagglutin ...201626769558
prevalence of ten putative virulence genes in the emerging foodborne pathogen arcobacter isolated from food products.arcobacter spp. are considered to be emerging food- and waterborne pathogens for both humans and animals. however, their virulence mechanisms are still poorly understood. in this study the presence of ten virulence genes (cadf, ciab, cj1349, heca, hecb, mvin, plda, irga, tlya and iroe) was assessed in a set of 47 strains of arcobacter butzleri, 10 of arcobacter cryaerophilus and 1 arcobacter skirrowii strain recovered from different food products (pork, chicken, beef, milk, clams and mussels). o ...201526338128
arcobacter butzleri, arcobacter cryaerophilus, and arcobacter skirrowii circulation in a dairy farm and sources of milk contamination.even though dairy cows are known carriers of arcobacter species and raw or minimally processed foods are recognized as the main sources of human arcobacter infections in industrialized countries, data on arcobacter excretion patterns in cows and in milk are scant. this study aimed to identify potentially pathogenic arcobacter species in a dairy herd and to investigate the routes of arcobacter transmission among animals and the potential sources of cattle infection and milk contamination. a strat ...201526002896
modified isolation method of arcobacter spp. from different environmental and food samples.this study provides information on the occurrence of arcobacter in several types of water and food products of animal origin in the czech republic. we processed 190 samples using the modified method, and the occurrence of arcobacter spp. was confirmed in 36.8 % of these. this total incidence consisted of arcobacter butzleri (27.3 %), arcobacter cryaerophilus (8.4 %) and arcobacter skirrowii (1.1 %). we newly described the common presence of arcobacter spp. in sewage water in the czech republic t ...201525912846
insights in the pathogenesis and resistance of arcobacter: a review.arcobacter genus currently comprises 18 recognized species, among which arcobacter butzleri, arcobacter cryaerophilus and arcobacter skirrowii have been associated with human and animal disease. although these organisms, with special emphasis a. butzleri, are emerging as clinical pathogens, several aspects of their epidemiology and virulence are only starting to be clarified. in vitro human and animal cell culture assays have been used to show that several arcobacter species can adhere to and in ...201625806423
multilocus sequence typing reveals genetic diversity of foodborne arcobacter butzleri isolates in the north of spain.the emerging pathogen arcobacter butzleri is being increasingly isolated from different animal food products but the routes of its transmission to human are not well established yet. typing methods would be useful in gaining such knowledge. here we report the great genetic diversity observed among a. butzleri isolates from different food products. forty-five isolates were analyzed by multilocus sequence typing (mlst). a total of 157 alleles were identified across all seven loci, ranging from 16 ...201425261830
prevalence and antimicrobial susceptibility of arcobacter species in cow milk, water buffalo milk and fresh village cheese.in this study, the presence of arcobacter spp. was examined in cow milk (n=50), water buffalo (wb) milk (n=50) and fresh village cheese (n=50) samples. the 16s rdna-rflp method was used for the identification of arcobacter spp. the disc diffusion method was used to investigate the susceptibility of all strains identified to 18 different antimicrobial substances. the most commonly isolated arcobacter species were found to be arcobacter butzleri (38.89%), arcobacter cryaerophilus (22.23%) and arco ...201425064812
occurrence of six virulence-associated genes in arcobacter species isolated from various sources in shiraz, southern iran.in humans, arcobacters are associated with watery diarrhea and septicemia. although, recently, more cases of diarrhea have been caused by arcobacter species, very little is known about its pathogenesis. therefore, the aim of this study was to evaluate the presence of six putative arcobacter virulence genes (cadf, ciab, cj1349, mvin, plda, and tlya), in a set of 113 arcobacter butzleri, 40 arcobacter cryaerophilus, and 15 arcobacter skirrowii isolates that were recovered from various origins. the ...201424201143
prevalence and genetic diversity of arcobacter in food products in the north of spain.the bacterial contamination of food products can cause serious public health problems. interest in arcobacter contamination has increased due to the relationship between these bacteria and human enteritis. we studied the prevalence and genetic diversity of arcobacter species at the retail level in the province of alava in basque country, spain. the results showed a high genetic diversity and indicated the regular presence of the main arcobacter spp. associated with human enteric illness in food ...201323905804
isolation and identification of zoonotic species of genus arcobacter from chicken viscera obtained from retail distributors of the metropolitan area of san josé, costa rica.arcobacter is a genus of growing importance worldwide; some of its species are considered emerging enteropathogens and potential zoonotic agents. in costa rica, as well as in other countries, its isolation has been reported, so the objective of this project was to evaluate and identify the presence of arcobacter in chicken viscera sold in the metropolitan area of san josé, costa rica, as well as to determine the antimicrobial resistance patterns associated with it. one hundred fifty samples of c ...201323643133
occurrence and characteristics of fastidious campylobacteraceae species in porcine samples.this study investigated the prevalence and characteristics of campylobacteraceae including a range of fastidious species in porcine samples. over a thirteen month period caecal contents (n=402) and pork carcass swabs (n=401) were collected from three pork abattoirs and pork products (n=399) were purchased at point of sale in the republic of ireland. campylobacteraceae isolates were recovered by enrichment, membrane filtration and incubation in antibiotic free media under a modified atmosphere (3 ...201323474652
prevalence and distribution of arcobacter spp. in raw milk and retail raw beef.a total of 106 beef samples which consisted of local (n = 59) and imported (n = 47) beef and 180 milk samples from cows (n = 86) and goats (n = 94) were collected from selangor, malaysia. overall, 30.2% (32 of 106) of beef samples were found positive for arcobacter species. imported beef was significantly more contaminated (46.80%) than local beef (16.9%). arcobacter butzleri was the species isolated most frequently from imported (81.8%) and local (60%) beef, followed by arcobacter cryaerophilus ...201222856572
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