| polyphasic taxonomic study of the emended genus arcobacter with arcobacter butzleri comb. nov. and arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. | the relationships of 77 aerotolerant arcobacter strains that were originally identified as campylobacter cryaerophila (now arcobacter cryaerophilus [p. vandamme, e. falsen, r. rossau, b. hoste, p. segers, r. tytgat, and j. de ley, int. j. syst. bacteriol. 41:88-103, 1991]) and 6 reference strains belonging to the taxa arcobacter nitrofigilis, arcobacter cryaerophilus, and "campylobacter butzleri" were studied by using a polyphasic approach, in which we performed dna-rrna hybridizations, dna-dna ... | 1992 | 1503968 |
| revision of campylobacter, helicobacter, and wolinella taxonomy: emendation of generic descriptions and proposal of arcobacter gen. nov. | hybridization experiments were carried out between dnas from more than 70 strains of campylobacter spp. and related taxa and either 3h-labeled 23s rrnas from reference strains belonging to campylobacter fetus, campylobacter concisus, campylobacter sputorum, campylobacter coli, and campylobacter nitrofigilis, an unnamed campylobacter sp. strain, and a wolinella succinogenes strain or 3h- or 14c-labeled 23s rrnas from 13 gram-negative reference strains. an immunotyping analysis of 130 antigens ver ... | 1991 | 1704793 |
| specific detection and confirmation of campylobacter jejuni by dna hybridization and pcr. | conventional detection and confirmation methods for campylobacter jejuni are lengthy and tedious. a rapid hybridization protocol in which a 1,475-bp chromogen-labelled dna probe (pdt1720) and campylobacter strains filtered and grown on 0.22-micron-pore-size hydrophobic grid membrane filters (hgmfs) are used was developed. among the environmental and clinical isolates of c. jejuni, campylobacter coli, campylobacter jejuni subsp. doylei, campylobacter lari, and arcobacter nitrofigilis and a panel ... | 1997 | 9361442 |
| arcobacter cibarius sp. nov., isolated from broiler carcasses. | twenty gram-negative, rod-shaped, slightly curved, non-spore-forming bacteria that gave a negative result in arcobacter species-specific pcr tests but that yielded an amplicon in an arcobacter genus-specific pcr test were isolated from 13 unrelated broiler carcasses. numerical analysis of the profiles obtained by sds-page of whole-cell proteins clustered all isolates in a single group distinct from the other arcobacter species. dna-dna hybridization among four representative strains exhibited dn ... | 2005 | 15774649 |
| arcobacter halophilus sp. nov., the first obligate halophile in the genus arcobacter. | a gram-negative bacterium, designated la31b(t), was isolated from water collected from a hypersaline lagoon on laysan atoll in the north-western hawaiian islands. single cells of la31b(t) were slightly curved but became helical as their length increased. preliminary characterization based on 16s rrna gene sequence analysis showed that la31b(t) shared 96.0 % identity with an arcobacter sp. isolated from a cyanobacterial mat in hypersaline lake sinai, and 94 % identity with arcobacter nitrofigilis ... | 2005 | 15879267 |
| development of a real-time fluorescence resonance energy transfer pcr to detect arcobacter species. | a real-time pcr targeting the gyrase a subunit gene outside the quinolone resistance-determining region has been developed to detect arcobacter species. the species identification was done by probe hybridization and melting curve analysis, using fluorescence resonance energy transfer technology. discrimination between arcobacter species was straightforward, as the corresponding melting points showed significant differences with the characteristic melting temperatures of 63.5 degrees c, 58.4 degr ... | 2007 | 17652482 |
| arcobacter spp. possess two very short flagellins of which flaa is essential for motility. | like campylobacter and helicobacter spp., arcobacter spp. possess two flagellin genes (flaa and flab) located adjacent to each other. the aim of this study was to characterize the flagellin proteins of arcobacter spp., because these proteins are known virulence factors in the epsilonproteobacteria, to which these three species belong. with the exception of arcobacter nitrofigilis, arcobacter flagellins are almost half the size of those in other epsilonproteobacteria. arcobacter flagellin protein ... | 2008 | 18400014 |
| prevalence of arcobacter in meat and shellfish. | arcobacter is considered an emergent foodborne and waterborne enteropathogen. however, its prevalence in foods of animal origin is only partially known, because most studies have been concentrated on poultry, pork, and beef, and methods applied do not allow identification of all currently accepted arcobacter species. we investigated the prevalence of arcobacter in 203 food samples, 119 samples of seven different types of meats and 84 samples of four types of shellfish. isolates were identified i ... | 2009 | 19517742 |
| arcobacter defluvii sp. nov., isolated from sewage samples. | a polyphasic study was undertaken to clarify the taxonomic position of 12 isolates recovered from sewage samples. these isolates were recognized as a potential new species because a novel and specific pattern was produced with the 16s rdna-rflp arcobacter identification method. the sequences of the 16s rrna gene not only supported the classification of these strains in the genus arcobacter but also showed that they formed a separate phylogenetic line. strain sw28-11t chosen as representative of ... | 2010 | 20889767 |
| taxonomy, epidemiology, and clinical relevance of the genus arcobacter. | the genus arcobacter, defined almost 20 years ago from members of the genus campylobacter, has become increasingly important because its members are being considered emergent enteropathogens and/or potential zoonotic agents. over recent years information that is relevant for microbiologists, especially those working in the medical and veterinary fields and in the food safety sector, has accumulated. recently, the genus has been enlarged with several new species. the complete genomes of arcobacte ... | 2011 | 21233511 |
| complete genome sequence of arcobacter nitrofigilis type strain (ci). | arcobacter nitrofigilis (mcclung et al. 1983) vandamme et al. 1991 is the type species of the genus arcobacter in the family campylobacteraceae within the epsilonproteobacteria. the species was first described in 1983 as campylobacter nitrofigilis [1] after its detection as a free-living, nitrogen-fixing campylobacter species associated with spartina alterniflora loisel roots [2]. it is of phylogenetic interest because of its lifestyle as a symbiotic organism in a marine environment in contrast ... | 2010 | 21304714 |
| the use of two culturing methods in parallel reveals a high prevalence and diversity of arcobacter spp. in a wastewater treatment plant. | the genus arcobacter includes species considered emerging food and waterborne pathogens. despite arcobacter has been linked to the presence of faecal pollution, few studies have investigated its prevalence in wastewater, and the only isolated species were arcobacter butzleri and arcobacter cryaerophilus. this study aimed to establish the prevalence of arcobacter spp. at a wwtp using in parallel two culturing methods (direct plating and culturing after enrichment) and a direct detection by m-pcr. ... | 2016 | 27981053 |
| prevalence and antimicrobial susceptibility of arcobacter species in cow milk, water buffalo milk and fresh village cheese. | in this study, the presence of arcobacter spp. was examined in cow milk (n=50), water buffalo (wb) milk (n=50) and fresh village cheese (n=50) samples. the 16s rdna-rflp method was used for the identification of arcobacter spp. the disc diffusion method was used to investigate the susceptibility of all strains identified to 18 different antimicrobial substances. the most commonly isolated arcobacter species were found to be arcobacter butzleri (38.89%), arcobacter cryaerophilus (22.23%) and arco ... | 2014 | 25064812 |