| specific detection of campylobacter jejuni and campylobacter coli by using polymerase chain reaction. | development of a routine detection assay for campylobacter jejuni and campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction (pcr). an oligonucleotide primer pair from a conserved 5' region of the flaa gene of c. coli vc167 was used to amplify a 450-bp region by pcr. the primer pair specifically detected 4 strains of c. coli and 47 strains of c. jejuni; but it did not detect strains of campylobacter fetus, campylobacter lari, campylobacter upsaliensis, cam ... | 1992 | 1400961 |
| identification of some uncommon monounsaturated fatty acids of bacteria. | location of the double-bond position of monounsaturated fatty acids of various bacteria was accomplished with combined gas chromatography-mass spectrometry analysis of dimethyl disulfide (dmds) derivatives. the monoenoic fatty acids from whole cells were converted to methyl esters and then to dmds adducts and analyzed by capillary gas chromatography-mass spectrometry. the mass spectra of dmds adducts gave an easily recognizable molecular ion and two major diagnostic ions attributable to fragment ... | 1992 | 1401029 |
| polyphasic taxonomic study of the emended genus arcobacter with arcobacter butzleri comb. nov. and arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. | the relationships of 77 aerotolerant arcobacter strains that were originally identified as campylobacter cryaerophila (now arcobacter cryaerophilus [p. vandamme, e. falsen, r. rossau, b. hoste, p. segers, r. tytgat, and j. de ley, int. j. syst. bacteriol. 41:88-103, 1991]) and 6 reference strains belonging to the taxa arcobacter nitrofigilis, arcobacter cryaerophilus, and "campylobacter butzleri" were studied by using a polyphasic approach, in which we performed dna-rrna hybridizations, dna-dna ... | 1992 | 1503968 |
| restriction fragment length polymorphisms in the ribosomal genes for species identification and subtyping of aerotolerant campylobacter species. | whole-cell chromosomal digests of 84 strains of aerotolerant campylobacter (ac) were examined by using pvuii restriction fragment length polymorphisms of rrna genes followed by hybridization with escherichia coli 16s and 23s rrna (ribotyping). the ac strains belonged to campylobacter cryaerophila (n = 13) and a newly defined species, "c. butzleri" (n = 64). strains of c. cryaerophila belonged to two hybridization groups: dna group 1a (including the type strain of c. cryaerophila) and dna group 1 ... | 1991 | 1684797 |
| revision of campylobacter, helicobacter, and wolinella taxonomy: emendation of generic descriptions and proposal of arcobacter gen. nov. | hybridization experiments were carried out between dnas from more than 70 strains of campylobacter spp. and related taxa and either 3h-labeled 23s rrnas from reference strains belonging to campylobacter fetus, campylobacter concisus, campylobacter sputorum, campylobacter coli, and campylobacter nitrofigilis, an unnamed campylobacter sp. strain, and a wolinella succinogenes strain or 3h- or 14c-labeled 23s rrnas from 13 gram-negative reference strains. an immunotyping analysis of 130 antigens ver ... | 1991 | 1704793 |
| biochemical and serological characterization of campylobacter cryaerophila. | sixty-two isolates of campylobacter cryaerophila were recovered from aborted porcine and bovine fetuses, from porcine, bovine, and equine feces, and from different tissues of a dead piglet. phenotypic characterization was carried out on all isolates, and the results were compared with those obtained with the reference strains of c. cryaerophila, c. jejuni, c. coli, c. laridis, and c. hyointestinalis. the ability of c. cryaerophila strains to grow under aerobic conditions at 16 degrees c was foun ... | 1991 | 1993768 |
| campylobacter butzleri sp. nov. isolated from humans and animals with diarrheal illness. | seventy-eight aerotolerant campylobacter isolates were characterized phenotypically and by dna hybridization (hydroxyapatite method at 50 and 65 degrees c). two dna relatedness groups were found. (i) sixty-four strains belonged to aerotolerant campylobacter dna hybridization group 2. these organisms were isolated from humans, primarily with diarrheal illness, and animals on several continents. strains were aerotolerant at 30 and 36 degrees c and catalase negative or weakly catalase positive, gre ... | 1991 | 2007646 |
| isolation of group 2 aerotolerant campylobacter species from thai children with diarrhea. | campylobacter species were isolated from 93 (15%) of 631 thai children with diarrhea using the membrane filter technique on nonselective blood agar incubated at 37 degrees c. campylobacter jejuni was isolated from 62 (10%), campylobacter coli from 14 (2%), and atypical campylobacters from 17 (3%). the 17 atypical strains were first characterized biochemically and by dot blot dna hybridization. catalase-negative strains also were characterized by dna hybridization and ribotype pattern. one strain ... | 1991 | 2019754 |
| isoprenoid quinones of campylobacter cryaerophila, c. cinaedi, c. fennelliae, c. hyointestinalis, c. pylori, and "c. upsaliensis". | the isoprenoid quinone contents of campylobacter cryaerophila, c. cinaedi, c. fennelliae, c. hyointestinalis, c. pylori, and "c. upsaliensis" were determined by reverse-phase thin-layer and high-performance liquid chromatography. all six of these recently named campylobacter species contained menaquinone-6 (mk-6), but only c. hyointestinalis and "c. upsaliensis" contained 2,[5 or 8]-dimethyl-3-farnesyl-farnesyl-1,4-naphthoquinone (*mk-6), a previously described novel menaquinone of the campyloba ... | 1990 | 2312688 |
| location of double bonds in monounsaturated fatty acids of campylobacter cryaerophila with dimethyl disulfide derivatives and combined gas chromatography-mass spectrometry. | location of the double-bond position of monounsaturated fatty acids in campylobacter cryaerophila was accomplished with combined gas chromatography-mass spectrometry analysis of dimethyl disulfide (dmds) derivatives. the monoenoic fatty acids from whole bacterial cells were converted to methyl esters and then to dmds adducts and analyzed by capillary gas chromatography-mass spectrometry. the mass spectra of dmds adducts gave an easily recognizable molecular ion (m+) and two major diagnostic ions ... | 1989 | 2768436 |
| campylobacter cryaerophila isolated from a human. | campylobacter cryaerophila was recovered from a single stool specimen of a 35-year-old homosexual man who presented with intermittent diarrhea for 4 to 6 months. the isolate was identified as c. cryaerophila by using biochemical reactions and confirmed by dna-dna hybridization and gas-liquid chromatograph profiles. the base composition of dna of the isolate was found to be 31.1 +/- 1 mol% (g+c). c. cryaerophila was previously reported to be associated with bovine and porcine abortions in animals ... | 1988 | 3230125 |
| arcobacter-specific and arcobacter butzleri-specific 16s rrna-based dna probes. | the genus arcobacter encompasses gram-negative, aerotolerant, spiral-shaped bacteria formerly designated campylobacter cryaerophila. two genus-specific 16s rrna-based oligonucleotide dna probes (23-mer and 27-mer) were developed. the probes hybridized with strains of arcobacter butzleri (n = 58), arcobacter cryaerophilus (n = 19), and arcobacter skirrowii (n = 17). the probes did not cross-react with any of the reference strains of campylobacter, helicobacter, including "flexispira rappini," or ... | 1995 | 7545177 |
| arcobacter cryaerophilus and thermophilic campylobacters in a sewage treatment plant in italy: two secondary treatments compared. | microaerophilic organisms were monitored in sewage effluent undergoing two secondary treatments: air and oxygen-activated sludge. the mean numbers of arcobacter cryaerophilus and thermophilic campylobacters detected in incoming sewage were 5639/100 ml and 1720/100 ml respectively. secondary treatment in air tanks reduced the population of a. cryaerophilus by 97.1% and of thermophilic campylobacters by 99.08%, whereas treatment in oxygen tanks reduced the bacteria 97.8% and 99.63% respectively, s ... | 1993 | 8519328 |
| toxigenic and invasive capacities: possible pathogenic mechanisms in arcobacter cryaerophilus. | | 1995 | 8569478 |
| infection of cesarean-derived colostrum-deprived 1-day-old piglets with arcobacter butzleri, arcobacter cryaerophilus, and arcobacter skirrowii. | neonatal piglets have been used as models to study human campylobacteriosis and helicobacteriosis. the purpose of this study was to determine the relative pathogenicities, on the basis of the duration of fecal shedding and colonization of tissues, of three arcobacter species in 1-day-old cesarean-derived colostrum-deprived piglets. two experiments were conducted. in experiment 1, two piglets each were infected per os with either arcobacter butzleri atcc 49616, arcobacter cryaerophilus 1b atcc 43 ... | 1996 | 8675340 |
| phenotypic and ribosomal rna characterization of arcobacter species isolated from porcine aborted fetuses. | aerotolerant organisms resembling campylobacter, now designated as arcobacter, have been described from aborted farm animals and from cases of human enteritis worldwide. the goals of this study were 1) to attempt to recover arcobacter spp. from cases of porcine abortion, 2) to characterize these isolates by phenotype and ribotype, and 3) to compare the usefulness of ribotype and phenotype patterns for identifying arcobacter butzleri and the dna hybridization groups 1a and 1b of a. cryaerophilus. ... | 1996 | 8744740 |
| identification of arcobacter isolates by pcr. | a polymerase chain reaction (pcr) assay was developed for the identification of the three species of arcobacter which have been recovered from clinically ill or healthy humans and/or livestock, namely arcobacter butzleri, arcobacter skirrowii and arcobacter cryaerophilus. the assay utilizes primers targeted to the genes encoding 16s rrna of arcobacter spp. the assay reduces the amount of time required to positively identify strains of arcobacter. | 1996 | 8987697 |
| bacteremia caused by arcobacter cryaerophilus 1b. | arcobacter cryaerophilus group 1b, a gram-negative, curved or helical bacillus primarily known as a bovine and porcine pathogen, was isolated from the blood of a uremic patient with hematogenous pneumonia. the patient was treated successfully with ceftizoxime and tobramycin. | 1997 | 9003624 |
| classification of arcobacter species isolated from aborted pig fetuses and sows with reproductive problems in brazil. | seventeen field isolates of arcobacter species were recovered in brazil from aborted porcine fetal livers (n = 3), kidneys (n = 2), and thoracic fluid (n = 1). arcobacter species were also recovered from uterine and oviductal tissues (n = 5) and a placenta from sows with reproductive problems. these isolates were initially presumed to be arcobacter cryaerophilus on the basis of aerobic growth at 30 degree c, indoxyl acetate hydrolysis, catalase and oxidase reactions, growth on macconkey agar, se ... | 1997 | 9444071 |
| comparison of the productivity of cefoperazone amphotericin teicoplanin (cat) agar and modified charcoal cefoperazone deoxycholate (mccd) agar for various strains of campylobacter, arcobacter and helicobacter pullorum. | cefoperazone amphotericin teicoplanin (cat) agar was developed from cefoperazone deoxycholate (mccd) agar by modification of the selective antibiotics in order to permit growth of strains of campylobacter upsaliensis. in this study, 35 strains of campylobacter and arcobacter were tested for their ability to grow on cat and mccd media using the ecometric method. six of these strains were also tested using the modified miles-misra method. overall, nineteen strains out of the 35 tested grew better ... | 1997 | 9506285 |
| prevalence of campylobacter, arcobacter, helicobacter, and sutterella spp. in human fecal samples as estimated by a reevaluation of isolation methods for campylobacters. | the aims of this study were to investigate the prevalence of campylobacteria including campylobacter jejuni subsp. jejuni (c. jejuni) and campylobacter coli in human clinical samples and in samples from healthy individuals and to reevaluate the efficacies of conventional selective methods for isolation of campylobacter spp. two charcoal-based selective media, modified charcoal cefoperazone deoxycholate agar (mccda) and cefoperazone-amphotericin-teicoplanin (cat) agar, were compared with skirrow' ... | 2000 | 10618103 |
| development of a multiplex pcr assay for the simultaneous detection and identification of arcobacter butzleri, arcobacter cryaerophilus and arcobacter skirrowii. | a multiplex pcr assay with five primers targeting the 16s and 23s rrna genes was developed for the simultaneous detection and identification of arcobacter butzleri, arcobacter cryaerophilus and arcobacter skirrowii. the selected primers amplify a 257-bp fragment from a. cryaerophilus, a 401-bp fragment from a. butzleri and a 641-bp fragment from a. skirrowii. no pcr product was generated for closely related bacteria including campylobacter and helicobacter species. the assay was useful to identi ... | 2000 | 11094284 |
| susceptibility of arcobacter butzleri, arcobacter cryaerophilus, and arcobacter skirrowii to antimicrobial agents used in selective media. | several antimicrobial agents used in selective media for the isolation of arcobacter were found to be inhibitory to strains belonging to this genus. all three species tested were susceptible to colistin and rifampin at concentrations used in selective media. arcobacter skirrowii was the most susceptible species. 5-fluorouracil, novobiocin, trimethoprim, and teicoplanin or vancomycin were found to be without any inhibitory effect on the strains tested at concentrations described for the isolation ... | 2001 | 11283110 |
| identification of arcobacter cryaerophilus isolated from a traffic accident victim with bacteremia by 16s ribosomal rna gene sequencing. | traditional ways of identifying slow growing bacteria is slow and often difficult. in this study, a small, gram-negative, facultative anaerobic, slow growing bacillus was isolated from the blood culture of a 7-year old traffic accident victim. the bacterium was non-hemolytic, catalase and oxidase positive. an attempt to use the vitek system (gni+) and the api system (20ne) to identify the strain was unsuccessful as the growth controls showed negative results. 16s ribosomal rna gene sequencing sh ... | 2001 | 11502381 |
| assessment of the genetic diversity among arcobacters isolated from poultry products by using two pcr-based typing methods. | in this study, enterobacterial repetitive intergenic consensus pcr (eric-pcr) and randomly amplified polymorphic dna pcr (rapd-pcr) were optimized for characterization of arcobacter butzleri, arcobacter cryaerophilus, and arcobacter skirrowii. in addition, a simple and rapid dna extraction method was tested for use in both typing procedures. both methods had satisfactory typeability and discriminatory power, but the fingerprints generated with eric-pcr were more reproducible and complex than tho ... | 2002 | 11976086 |
| occurrence and distribution of arcobacter species in poultry processing. | a total of 16 broiler flocks slaughtered in the morning in eight belgian poultry slaughterhouses were examined for the presence of campylobacteraceae. in samples collected before and after chilling, the prevalence of arcobacters was found to be higher than the prevalence of thermophilic campylobacters, with the slaughter procedure used having no clear effect. two slaughterhouses were selected for a detailed investigation of the occurrence and distribution of arcobacters. sampling carried out bef ... | 2002 | 12182473 |
| sensitivity of three methods used in the isolation of arcobacter spp. in raw ground pork. | arcobacter, an aerotolerant campylobacter-like organism, has been designated an emerging pathogen because of its newly recognized ability to cause diarrheal illness in both humans and animals and its presence in the human food supply. because there is no standard isolation method for its detection, the true occurrence of this pathogen is largely unknown. in addition, the lack of a standardized isolation protocol limits the ability of investigators to compare field data. arcobacter has been detec ... | 2002 | 12430704 |
| one-step polymerase chain reaction-based typing of arcobacter species. | a species-specific pcr assay was developed for the identification of the arcobacter species, arcobacter butzleri, arcobacter cryaerophilus 1a and 1b, and arcobacter skirrowii. the primers, which amplify the most variable areas of the 23s rrna gene, were designed to perform species-specific identification by one-step pcr. the dna sequence of the region from a. cryaerophilus 1b was determined, and the specific pimer for the species was designed. by using one-step pcr containing the mixed primers n ... | 2003 | 12457591 |
| molecular characterization of arcobacter isolates collected in a poultry slaughterhouse. | in a poultry slaughterhouse, arcobacter contamination was examined over a period of 1 week to establish possible routes of contamination. samples were collected from the slaughter equipment and from processing water before the onset of slaughter and from the first broiler flock slaughtered on each sampling day. characterization of 1,079 isolates by enterobacterial repetitive intergenic consensus-polymerase chain reaction and a random amplified polymorphic dna assay resulted in the delineation of ... | 2003 | 12636286 |
| in vitro susceptibility of arcobacter butzleri and arcobacter cryaerophilus to different antimicrobial agents. | seventeen strains of arcobacter butzleri and thirteen of arcobacter cryaerophilus, were tested for their antimicrobial susceptibility to 26 antimicrobial agents. among beta-lactams agents in this study, imipenem was the most active agent against both a. butzleri and a. cryaerophilus isolates with mic(90) values of 2 and 4 mg/l, respectively. the most active cephalosporin tested was cefepime, although it was more active against a. butzleri (mic(90) 8 mg/l) than a. cryaerophilus (mic(90) 64 mg/l). ... | 2003 | 12727085 |
| usefulness of the microseq 500 16s ribosomal dna-based bacterial identification system for identification of clinically significant bacterial isolates with ambiguous biochemical profiles. | due to the inadequate automation in the amplification and sequencing procedures, the use of 16s rrna gene sequence-based methods in clinical microbiology laboratories is largely limited to identification of strains that are difficult to identify by phenotypic methods. in this study, using conventional full-sequence 16s rrna gene sequencing as the "gold standard," we evaluated the usefulness of the microseq 500 16s ribosomal dna (rdna)-based bacterial identification system, which involves amplifi ... | 2003 | 12734240 |
| arcobacter cryaerophilus and arcobacter butzleri isolated from preputial fluid of boars and fattening pigs in brazil. | | 1999 | 12968762 |
| detection of arcobacter spp. in the coastal environment of the mediterranean sea. | the occurrence of arcobacter spp. was studied in seawater and plankton samples collected from the straits of messina, italy, during an annual period of observation by using cultural and molecular techniques. a pcr assay with three pairs of primers targeting the 16s and 23s rrna genes was used for detection and identification of arcobacter butzleri, arcobacter cryaerophilus, and arcobacter skirrowii in cultures and environmental samples. only one of the arcobacter species, a. butzleri, was isolat ... | 2004 | 15006743 |
| a comparison of three methods for the isolation of arcobacter spp. from retail raw poultry in northern ireland. | recent evidence suggests that arcobacters, especially arcobacter butzleri, are potential foodborne pathogens, but standardized detection methods have yet to be established. a study was undertaken to determine which of three isolation methods was the most effective for the isolation of arcobacter spp. from fresh raw poultry. methods 1 was microaerobic and involved a membrane filtration step followed by plating onto blood agar. method 2 was also microaerobic and involved enrichment and plating med ... | 2004 | 15083734 |
| antimicrobial susceptibility patterns of arcobacter butzleri and arcobacter cryaerophilus strains isolated from humans and broilers. | the mics of five antimicrobial agents were determined by the agar dilution method for 98 arcobacter butzleri and 28 arcobacter cryaerophilus strains from humans, and poultry. with gentamicin, a mic of 16 microg/ml was recorded for one a. butzleri strain isolated from poultry, whereas for the other strains mics ranged from 0.25 to 4 microg/ml. with ciprofloxacin, a bimodal distribution of susceptibility levels was seen for human a. butzleri isolates (0.015-0.03 versus 0.12-0.25), whereas mics for ... | 2004 | 15383169 |
| prevalence, enumeration and strain variation of arcobacter species in the faeces of healthy cattle in belgium. | arcobacter species were isolated from faeces of healthy cattle on three unrelated belgian farms, using a quantitative isolation protocol. isolates were identified by m-pcr and characterized by modified eric-pcr. the arcobacter prevalence on the three farms ranged from 7.5 to 15%. the prevalence in dairy cattle ranged from 5.9 to 11% and for young cattle and calves, the prevalence was determined as 18.9 and 27.3%, respectively. of the 276 animals examined, eight had a bacterial load of more than ... | 2004 | 15627527 |
| effect of ph, nacl content, and temperature on growth and survival of arcobacter spp. | growth and survival of six human isolates of the pathogenic arcobacter spp. in the presence of selected environmental factors were studied. four strains of arcobacter butzleri and two strains of arcobacter cryaerophilus were exposed to ph levels of 3.5 to 8.0. most strains grew between ph 5.5 and 8.0, with optimal growth of most a. butzleri and a. cryaerophilus strains at ph 6.0 to 7.0 and 7.0 to 7.5, respectively. the 24-h optimal growth range in the presence of nacl was 0.5 to 1.0% for a. crya ... | 2005 | 15690799 |
| investigation of bovine venereal campyloacteriosis in beef cow herds in new zealand. | to determine regional prevalences of beef cow herds in new zealand positive for campylobacter fetus subsp venerealis antibodies in samples of vaginal mucus tested using an immunoglobulin (ig) a enzyme-linked immunosorbent assay (elisa), and to examine the suitability of the iga elisa for detecting infection with c. fetus subsp venerealis under field conditions in new zealand. | 2005 | 15731834 |
| arcobacter cibarius sp. nov., isolated from broiler carcasses. | twenty gram-negative, rod-shaped, slightly curved, non-spore-forming bacteria that gave a negative result in arcobacter species-specific pcr tests but that yielded an amplicon in an arcobacter genus-specific pcr test were isolated from 13 unrelated broiler carcasses. numerical analysis of the profiles obtained by sds-page of whole-cell proteins clustered all isolates in a single group distinct from the other arcobacter species. dna-dna hybridization among four representative strains exhibited dn ... | 2005 | 15774649 |
| detection and diversity of various arcobacter species in danish poultry. | the prevalence and diversity of different arcobacter spp. in various poultry species in denmark were investigated using cultural and multiplex pcr methods. a pool of three fresh droppings obtained at the production site from 70 broiler chicken flocks aged 4-5 weeks was examined. in addition, pools of 10 cloacal swabs taken at the abattoir prior to stunning from each of 15, and 37 duck and turkey flocks, respectively, were analyzed. thirty fresh broiler chicken carcasses and 29 cloacal swabs from ... | 2006 | 16516995 |
| antimicrobial susceptibility of clinical isolates of non-jejuni/coli campylobacters and arcobacters from belgium. | to determine the susceptibility of non-jejuni/coli campylobacters and arcobacters isolated from diarrhoeal stool specimens in belgium. | 2006 | 16533825 |
| arcobacter, what is known and unknown about a potential foodborne zoonotic agent! | since the introduction of the genus arcobacter in 1991, the association of arcobacter butzleri, arcobacter cryaerophilus and arcobacter skirrowii with humans and animals has been clearly established. these bacteria have been detected world wide in products of animal origin and in healthy animals as well as in surface water. a fourth species arcobacter cibarius was recently discovered on chicken carcasses. although evidence was found for the connection of arcobacter spp. with human and animal ill ... | 2006 | 16621345 |
| genetic diversity of arcobacter and campylobacter on broiler carcasses during processing. | broiler carcasses (n=325) were sampled at three sites along the processing line (prescalding, prechilling, and postchilling) in a commercial poultry processing plant during five plant visits from august to october 2004. pulsed-field gel electrophoresis (pfge) was used to determine the genomic fingerprints of camospylobacter coli (n=27), campylobacter jejuni (n=188), arcobacter butzleri (n=138), arcobacter cryaerophilus 1a (n=4), and a. cryaerophilus 1b (n=31) with the restriction enzymes smai an ... | 2006 | 16715800 |
| pathological effects of arcobacter cryaerophilus infection in rainbow trout (oncorhynchus mykiss walbaum). | arcobacter cryaerophilus was isolated from naturally infected rainbow trout (oncorhynchus mykiss walbaum), and its pathogenicity was tested by intramuscular injection into 40 healthy 1-year-old rainbow trout at 16 degrees c. the lethal dosage of 50% end point (ld50) for a. cryaerophilus was calculated 2.25 x 10(4) viable cells. experimental infection caused deaths with gross clinical abnormalities such as degenerated opercula and gills, liver damage, haemorrhagic kidney and serous fluid in swoll ... | 2006 | 16841757 |
| prevalence of arcobacter spp. in raw milk and retail raw meats in northern ireland. | a 1-year study was undertaken to determine the prevalence of arcobacter spp. in raw milk and retail raw meats on sale in northern ireland. retail raw poultry samples (n = 94), pork samples (n = 101), and beef samples (n = 108) were obtained from supermarkets in northern ireland, and raw milk samples (n = 101) were kindly provided by the milk research laboratory, department of agriculture and rural development, belfast, northern ireland. presumptive arcobacters were identified by previously descr ... | 2006 | 16924929 |
| differential regulation of porcine beta-defensins 1 and 2 upon salmonella infection in the intestinal epithelial cell line ipi-2i. | intestinal epithelial cells represent the first line of defence against pathogenic bacteria in the lumen of the gut. besides acting as a physical barrier, epithelial cells orchestrate the immune response through the production of several innate immune mediator molecules including beta-defensins. here, we establish the porcine intestinal cell line ipi-2i as a new model system to test the regulation of porcine beta-defensins 1 and 2. gene expression of both defensins was highly upregulated by foet ... | 2006 | 16938353 |
| development of a multiplex and real time pcr assay for the specific detection of arcobacter butzleri and arcobacter cryaerophilus. | a new multiplex pcr and two specific taqman assays were developed to target the emerging pathogens a. butzleri and a. cryaerophilus. the assays also included an internal control to verify the presence of bacterial target dna and amplification integrity. the multiplex assay used a published primer set (cry1 and cry2) for detecting a. cryaerophilus dna (houf, k., tutenel, a., de zutter, l., van hoof, j. and vandamme, p., 2000. development of a multiplex pcr assay for the simultaneous detection and ... | 2007 | 17055091 |
| isolation and characterization of the emerging foodborn pathogen arcobacter from human stool. | at present, isolation of arcobacters from human specimens is performed by slightly of not modified campylobacter, yersinia or leptospira isolation techniques, and knowledge if arcobacters are part of the human commensal flora is lacking. therefore, an arcobacter selective isolation procedure was validated for the examination of human fecal specimens, and the presence and characteristics of arcobacter in feces of asymptomatic humans was examined in order to assess the clinical relevance of arcoba ... | 2007 | 17097175 |
| direct detection and identification of arcobacter species by multiplex pcr in chicken and wastewater samples from spain. | twenty-two chicken livers, 10 chicken carcasses, and 15 wastewater samples were processed and analyzed for arcobacter by pcr and traditional culture methods. samples were enriched for 24 and 48 h, incubated at 30 degrees c under aerobic conditions, and streaked on blood selective media. to determine the best isolation conditions, 20 samples also were processed under microaerophilic conditions at 37 degrees c. simple and multiplex pcr assays were used directly with enrichment broths and isolated ... | 2007 | 17340867 |
| nucleotide sequence of the gyra gene of arcobacter species and characterization of human ciprofloxacin-resistant clinical isolates. | the nucleotide sequence of the gyra gene of arcobacter butzleri, arcobacter cryaerophilus, arcobacter cibarius, and arcobacter skirrowii was determined. the deduced gyra proteins are closely related to those of wolinella succinogenes and helicobacter pullorum, whereas those of campylobacter species showed less sequence identity. the phylogenetic analysis of gyra sequences provides a result similar to 16s rrna gene sequence phylogenetic analysis and allows the discrimination among a. butzleri spe ... | 2007 | 17378897 |
| prevalence and distribution of arcobacter species in various sources in turkey and molecular analysis of isolated strains by eric-pcr. | to determine the prevalence of arcobacter in various food, animal and water sources in turkey and to subtype the isolated strains using enterobacterial repetitive intergenic consensus (eric)-pcr. | 2007 | 17584450 |
| detection of arcobacter spp. in piggery effluent and effluent-irrigated soils in southeast queensland. | to investigate the occurrence and levels of arcobacter spp. in pig effluent ponds and effluent-treated soil. | 2007 | 17650202 |
| development of a real-time fluorescence resonance energy transfer pcr to detect arcobacter species. | a real-time pcr targeting the gyrase a subunit gene outside the quinolone resistance-determining region has been developed to detect arcobacter species. the species identification was done by probe hybridization and melting curve analysis, using fluorescence resonance energy transfer technology. discrimination between arcobacter species was straightforward, as the corresponding melting points showed significant differences with the characteristic melting temperatures of 63.5 degrees c, 58.4 degr ... | 2007 | 17652482 |
| evaluation of a cytolethal distending toxin (cdt) gene-based species-specific multiplex pcr assay for the identification of campylobacter strains isolated from poultry in thailand. | we have recently developed a cytolethal distending toxin (cdt) gene-based species-specific multiplex pcr assay for identifying campylobacter jejuni, c. coli and c. fetus. in the present study, the applicability of this assay was evaluated with 34 campylobacter-like organisms isolated from poultry in thailand for species identification and was compared with other assays including api campy, 16s rrna gene sequence, and hippuricase (hipo) gene detection. of the 34 strains analyzed, 20, 10 and 1 wer ... | 2007 | 17895609 |
| a new 16s rdna-rflp method for the discrimination of the accepted species of arcobacter. | the genus arcobacter that includes some species of clinical interest has been recently enlarged with the addition of several new species. the available molecular techniques for the characterization of arcobacter spp. are unable to identify all the species included in this genus. we have developed a 16s rdna-rflp method able to separate the currently accepted 6 species of arcobacter, including the 2 hybridization groups of arcobacter cryaerophilus. the method based on the use of a pair of primers ... | 2008 | 18060724 |
| presence of arcobacter spp. in environmental waters correlates with high levels of fecal pollution. | we investigated the presence of arcobacter spp. in 205 water samples of freshwater, seawater and sewage in spain. these bacteria were present in 55.1% of the samples (113/205) and were significantly associated for the first time with bacterial indicators of fecal pollution. the dominant species in the positive samples was arcobacter butzleri (94%) followed by arcobacter cryaerophilus (30%) and arcobacter skirrowii (1.8%). | 2008 | 18215159 |
| inhibition of arcobacter butzleri, arcobacter cryaerophilus, and arcobacter skirrowii by plant oil aromatics. | the inhibitory effect of some plant oil aromatics against three strains of arcobacter butzleri, two strains of arcobacter cryaerophilus, and one strain of arcobacter skirrowii was evaluated. when mics were determined using the broth macrodilution method, cinnamaldehyde was most inhibitory followed by thymol, carvacrol, caffeic acid, tannic acid, and eugenol (p < 0.001). sublethal concentrations of the three most potent plant oil aromatics also were examined. overall, cinnamaldehyde was the most ... | 2008 | 18236678 |
| the presence of arcobacter species in breeding hens and eggs from these hens. | the presence of arcobacter spp. in 2 breeding hen flocks was determined by examination of the intestinal tract, oviduct magnum mucosa, and ovarian follicles of slaughtered chicken. the bacteria were detected by pcr and cultural isolation in 34 out of 40 intestinal tracts from one flock (a) and 6 out of 30 from the other (b). the strains were arcobacter butzleri, arcobacter cryaerophilus, and arcobacter skirrowii. from flock a, arcobacters were recovered from 6 out of 40 oviduct magnum mucosa sam ... | 2008 | 18931194 |
| pet cats as carriers of arcobacter spp. in southern italy. | to evaluate the presence of arcobacter spp. in different biological samples from domestic cats in southern italy by using a species-specific pcr assay and thus to elucidate their potential significance as sources of human infection. | 2009 | 19226387 |
| prevalence of arcobacter in meat and shellfish. | arcobacter is considered an emergent foodborne and waterborne enteropathogen. however, its prevalence in foods of animal origin is only partially known, because most studies have been concentrated on poultry, pork, and beef, and methods applied do not allow identification of all currently accepted arcobacter species. we investigated the prevalence of arcobacter in 203 food samples, 119 samples of seven different types of meats and 84 samples of four types of shellfish. isolates were identified i ... | 2009 | 19517742 |
| gross pathology, blood chemistry, lipid and peroxide contents in rainbow trout ( oncorhynchus mykiss walbaum) affected by experimental arcobacter cryaerophilus infection at low water temperature. | arcobacter cryaerophilus was isolated from naturally infected rainbow trout ( oncorhynchus mykiss walbaum), and its pathogenicity was tested by intramuscular injection using healthy 1-year-old rainbow trout under cold-water conditions (at 5 degrees c). the lethal dosage of 50% end point (ld 50 ) for a. cryaerophilus was calculated as 7.79 x 10 5 viable cells. experimental infection caused gross clinical abnormalities such as fallen scales, exophthalmia, oedema in injection region and at the base ... | 2009 | 19584043 |
| arcobacter thereius sp. nov., isolated from pigs and ducks. | during a danish study on the prevalence of campylobacteria in pig abortions and food of animal origin, eight gram-negative, slightly curved, rod-shaped, non-spore-forming bacteria were clustered by using amplified fragment length polymorphism analysis in a distinct phenon within the genus arcobacter. in the present study, numerical analysis of whole-cell protein profiles also showed that all isolates clustered in a single group distinct from other recognized arcobacter species. dna-dna hybridiza ... | 2009 | 19622651 |
| specific pcr detection of arcobacter butzleri, arcobacter cryaerophilus, arcobacter skirrowii, and arcobacter cibarius in chicken meat. | an enrichment pcr assay using species-specific primers was developed for the detection of arcobacter butzleri, arcobacter cryaerophilus, arcobacter skirrowii, and arcobacter cibarius in chicken meat. primers for a. cryaerophilus, a. skirrowii, and a. cibarius were designed based on the gyra gene to amplify nucleic acid fragments of 212, 257, and 145 bp, respectively. the a. butzleri-specific primers were designed flanking a 203-bp dna fragment in the 16s rrna gene. the specificity of the four pr ... | 2009 | 19681276 |
| rapid and accurate detection of arcobacter contamination in commercial chicken products and wastewater samples by real-time polymerase chain reaction. | an sybr green real-time polymerase chain reaction (pcr) assay was developed for arcobacter detection in food and wastewater samples. the assay was applied to 36 chicken and 33 wastewater samples, and the results were compared with those obtained for conventional pcr, multiplex pcr, and culture isolation. isolates were identified by multiplex pcr and restriction fragment length polymorphism analysis of pcr-amplified dna fragment, and typed by randomly amplified polymorphic dna. arcobacter sp. was ... | 2010 | 19899959 |
| specific detection of arcobacter spp. in estuarine waters of southern italy by pcr and fluorescent in situ hybridization. | to evaluate the reliability of culture-independent methods in comparison with culture-dependent ones for the detection of arcobacter spp. in estuarine waters of southern italy. | 2010 | 19929906 |
| reassessment of the taxonomy of arcobacter cryaerophilus. | arcobacter cryaerophilus is a heterogeneous species in which two distinct subgroups have been reported. in the present study, the taxonomic status of these subgroups was reassessed using amplified fragment length polymorphism and heat shock protein 60 gene sequence analysis. the results demonstrated that a. cryaerophilus has a complex taxonomic structure, which consists of multiple cores of strains that share intermediate levels of dna-dna hybridisation and exhibit low levels of dna-dna hybridis ... | 2010 | 19945242 |
| arcobacter trophiarum sp. nov., isolated from fattening pigs. | in the course of a longitudinal study elucidating the dynamics of arcobacter populations in pigs, 16 isolates of gram-reaction-negative, rod-shaped, slightly curved, non-spore-forming bacteria were grouped by amplified fragment length polymorphism analysis into a distinct phenon within the genus arcobacter. fragments were generated for all isolates in a genus-specific pcr assay, but no amplicon was obtained in a species-specific multiplex-pcr test. numerical analysis of the whole-cell protein pr ... | 2011 | 20305065 |
| occurrence and diversity of arcobacter spp. along the llobregat river catchment, at sewage effluents and in a drinking water treatment plant. | the presence of arcobacter species in faecally contaminated environmental waters has previously been studied. however, the ability to eliminate arcobacter during the water treatment processes that produce drinking water has been little studied. we have investigated the prevalence and diversity of arcobacter spp. throughout the year at 12 sampling points in the llobregat river catchment (catalonia, spain) including 3 sites at a drinking water treatment plant. positive samples for arcobacter spp., ... | 2010 | 20427071 |
| prevalence of arcobacter species isolated from retail meats in korea. | this study was conducted to determine the prevalence of arcobacter species identified or isolated from retail meats in korea. multiplex pcr assays for the detection of arcobacter species were performed for 360 chicken, 100 pork, and 106 beef samples. arcobacter butzleri and arcobacter cryaerophilus were detected in 18.9 and 3.3% of chicken samples, respectively. however, arcobacter species were not found in any of the pork and beef samples. biochemical testing of isolates selected after enrichme ... | 2010 | 20615344 |
| inhibitory effect of organic acids on arcobacters in culture and their use for control of arcobacter butzleri on chicken skin. | the inhibitory effects of 17 organic acids (c₂-c₁₆ fatty acids, sorbic, benzoic, phenylacetic, fumaric, succinic, lactic, malic and citric) on arcobacter butzleri, arcobacter cryaerophilus and arcobacter skirrowii were investigated by determining their ic₅₀ values, defined as the concentration of acid at which the target dna sequence was expressed at 50% of the positive control level in cultures incubated at 30°c for 24 h. dna was analysed by real-time pcr. the arcobacter strains tested were inh ... | 2010 | 21084127 |
| occurrence of putative virulence genes in arcobacter species isolated from humans and animals. | interest for arcobacters in veterinary and human public health has increased since the first report of the isolation of arcobacters from food of animal origin. since then, studies worldwide have reported the occurrence of arcobacters on food and in food-production animals and have highlighted the possible transmission of especially a. butzleri to the human population. in humans, arcobacters are associated with enteritis and septicemia. to assess their clinical relevance for humans and animals, e ... | 2011 | 22170914 |
| a severe case of persistent diarrhoea associated with arcobacter cryaerophilus but attributed to campylobacter sp. and a review of the clinical incidence of arcobacter spp. | although rarely, arcobacter spp. have been associated with diarrhoea and bacteraemia. we report a persistent case in a healthy 26-year-old spanish male of bloody diarrhoea, which was attributed to campylobacter but in fact was caused by arcobacter cryaerophilus, as determined by sequencing of the rpob gene. the isolate was re-identified by matrix-assisted laser desorption ionization time of flight (maldi-tof) and genotyped for five putative virulence genes and for seven genes included in the arc ... | 2014 | 25356338 |
| comparison of conventional pcr, multiplex pcr, and loop-mediated isothermal amplification assays for rapid detection of arcobacter species. | this study aimed to develop a loop-mediated isothermal amplification (lamp) method for the rapid detection of arcobacter species. specific primers targeting the 23s ribosomal rna gene were used to detect arcobacter butzleri, arcobacter cryaerophilus, and arcobacter skirrowii. the specificity of the lamp primer set was assessed using dna samples from a panel of arcobacter and campylobacter species, and the sensitivity was determined using serial dilutions of arcobacter species cultures. lamp show ... | 2014 | 24478488 |
| presence and analysis of plasmids in human and animal associated arcobacter species. | in this study, we report the screening of four arcobacter species for the presence of small and large plasmids. plasmids were present in 9.9% of the 273 examined strains. one arcobacter cryaerophilus and four arcobacter butzleri plasmids were selected for further sequencing. the size of three small plasmids isolated from a. butzleri and the one from a. cryaerophilus strains ranged between 4.8 and 5.1 kb, and the size of the large plasmid, isolated from a. butzleri, was 27.4 kbp. the g+c content ... | 2014 | 24465575 |
| arcobacter butzleri: first isolation report from chicken carcasses in costa rica. | arcobacter butzleri isolation from chicken carcasses in costa rica is reported for the first time. the isolated strains (p and r) were presumptively identified by their phenotypic characteristics. definitive identification was made using a multiplex pcr assay for the simultaneous detection and identification of arcobacter butzleri, arcobacter cryaerophilus and arcobacter skirrowii. these first isolations indicate the necessity of further investigation about the prevalence, distribution, ecology ... | 2011 | 24031682 |
| arcobacter in lake erie beach waters: an emerging gastrointestinal pathogen linked with human-associated fecal contamination. | the genus arcobacter has been associated with human illness and fecal contamination by humans and animals. to better characterize the health risk posed by this emerging waterborne pathogen, we investigated the occurrence of arcobacter spp. in lake erie beach waters. during the summer of 2010, water samples were collected 35 times from the euclid, villa angela, and headlands (east and west) beaches, located along ohio's lake erie coast. after sample concentration, arcobacter was quantified by rea ... | 2012 | 22660704 |
| genetic diversity and incidence of virulence-associated genes of arcobacter butzleri and arcobacter cryaerophilus isolates from pork, beef, and chicken meat in poland. | incidence of 9 virulence-associated genes and genetic diversity was determined in 79 a. butzleri and 6 a. cryaerophilus isolates from pork, beef, and chicken meat. all a. butzleri isolates harboured the tlya gene, and most of them carried ciab, mvin, plda, cadf, and cj1349 genes. ciab was found to occur with higher frequency in poultry if compared with pork (p = 0.0007), while irga was more frequent in poultry than in beef (p = 0.007). all 6 a. cryaerophilus isolates harboured the ciab gene, whi ... | 2015 | 26539546 |
| arcobacter spp. in fecal samples from brazilian farmed caimans (caiman yacare, daudin 1802). | the aim of this study was to perform the identification and molecular characterization of arcobacter cryaerophilus and arcobacter butzleri isolated from caiman (caiman yacare), kept at a production farm, in brazil. forty fecal samples were analyzed. after isolation and identification, 21/40 strains of a. butzleri and 19/40 strains of a. cryaerophilus were subjected to pcr for potential virulence gene detection. the results of the pcr showed 38/40 strains positive for the cadf, cj1349, ciab, and ... | 2017 | 28321791 |
| detection, identification, and antimicrobial susceptibility of arcobacter spp. isolated from shellfish in spain. | this work aimed to determine the presence of arcobacter spp. in shellfish and to determine its susceptibility to quinolones. one hundred samples (41 mussels, 37 clams, and 22 cockles) were purchased from different local retail shops in valencia, spain, from september 2013 to june 2015. all samples were analyzed simultaneously by culture, after an enrichment step, and by polymerase chain reaction (pcr), directly and after enrichment. the susceptibility to levofloxacin and ciprofloxacin of the iso ... | 2017 | 28121468 |
| the use of two culturing methods in parallel reveals a high prevalence and diversity of arcobacter spp. in a wastewater treatment plant. | the genus arcobacter includes species considered emerging food and waterborne pathogens. despite arcobacter has been linked to the presence of faecal pollution, few studies have investigated its prevalence in wastewater, and the only isolated species were arcobacter butzleri and arcobacter cryaerophilus. this study aimed to establish the prevalence of arcobacter spp. at a wwtp using in parallel two culturing methods (direct plating and culturing after enrichment) and a direct detection by m-pcr. ... | 2016 | 27981053 |
| genetic diversity of arcobacter isolated from bivalves of adriatic and their interactions with mytilus galloprovincialis hemocytes. | the human food-borne pathogens arcobacter butzleri and a. cryaerophilus have been frequently isolated from the intestinal tracts and fecal samples of different farm animals and, after excretion, these microorganisms can contaminate the environment, including the aquatic one. in this regard, a. butzleri and a. cryaerophilus have been detected in seawater and bivalves of coastal areas which are affected by fecal contamination. the capability of bivalve hemocytes to interact with bacteria has been ... | 2017 | 27650799 |
| occurrence of emerging food-borne pathogenic arcobacter spp. isolated from pre-cut (ready-to-eat) vegetables. | given that changes in consumer food behaviours have led to an increase in the demand for pre-cut ready-to-eat (rte) vegetables, and that few data are currently available on the occurrence of arcobacter spp. in such foods, the aim of the present study was to assess the occurrence of arcobacter spp. that carry virulence-associated genes on pre-cut rte vegetables, using cultural and molecular methods. arcobacter was detected using biomolecular identification methods in 44/160 (27.5%) of the samples ... | 2016 | 27442848 |
| occurrence of potentially pathogenic arcobacters in shellfish. | considering that several recent cases of human gastroenteritis have been associated with species from the arcobacter genus, and that few data are currently available about the occurrence of this genus in italian shellfish, the aim of the present study was to evaluate the occurrence of arcobacter spp. and the presence of virulence-associated genes. the approach consisted of cultural and biomolecular (multiplex-pcr and 16s-rflp) methods identifying isolates, followed by pcr assays aimed at the cad ... | 2016 | 27052698 |
| antimicrobial susceptibility testing of arcobacter butzleri and arcobacter cryaerophilus strains isolated from belgian patients. | to evaluate the feasibility of different methods for susceptibility testing of human arcobacter isolates, to assess susceptibility to antibiotics commonly used to treat diarrhoeal illness and to obtain mic distribution data. | 2016 | 26851610 |
| development and evaluation of multiplex pcr assays for rapid detection of virulence-associated genes in arcobacter species. | as the pathogenicity of arcobacter species might be associated with various virulence factors, this study was aimed to develop and optimize three single-tube multiplex pcr (mpcr) assays that can efficiently detect multiple virulence-associated genes (vags) in arcobacter spp. including the arcobacter butzleri, arcobacter cryaerophilus and arcobacter skirrowii, respectively. the recognized target virulence factors used in the study were fibronectin binding protein (cj1349), filamentous hemagglutin ... | 2016 | 26769558 |
| [detection of biofilm formation by selected pathogens relevant to the food industry]. | detection of biofilm formation by microbial pathogens relevant to the food industry and comparison of biofilm formation under different conditions of culture. | 2015 | 26448305 |
| prevalence of ten putative virulence genes in the emerging foodborne pathogen arcobacter isolated from food products. | arcobacter spp. are considered to be emerging food- and waterborne pathogens for both humans and animals. however, their virulence mechanisms are still poorly understood. in this study the presence of ten virulence genes (cadf, ciab, cj1349, heca, hecb, mvin, plda, irga, tlya and iroe) was assessed in a set of 47 strains of arcobacter butzleri, 10 of arcobacter cryaerophilus and 1 arcobacter skirrowii strain recovered from different food products (pork, chicken, beef, milk, clams and mussels). o ... | 2015 | 26338128 |
| short communication: arcobacter butzleri and arcobacter cryaerophilus survival and growth in artisanal and industrial ricotta cheese. | ricotta cheese is a ready-to-eat product with properties (ph >6.0, aw >0.98-0.99) and moisture content (75-80%) that may pose a risk to public health due to postprocess contamination by several bacterial pathogens, including arcobacters. the objective of the study was to evaluate the behavior of arcobacter butzleri and arcobacter cryaerophilus in ricotta cheese during its shelf life assuming postprocessing contamination. two types of ricotta cheese, artisanal water buffalo (wb) and industrial co ... | 2015 | 26233450 |
| arcobacter butzleri, arcobacter cryaerophilus, and arcobacter skirrowii circulation in a dairy farm and sources of milk contamination. | even though dairy cows are known carriers of arcobacter species and raw or minimally processed foods are recognized as the main sources of human arcobacter infections in industrialized countries, data on arcobacter excretion patterns in cows and in milk are scant. this study aimed to identify potentially pathogenic arcobacter species in a dairy herd and to investigate the routes of arcobacter transmission among animals and the potential sources of cattle infection and milk contamination. a strat ... | 2015 | 26002896 |
| prevalence and antimicrobial resistance of arcobacter butzleri and arcobacter cryaerophilus isolates from retail meat in lower silesia region, poland. | arcobacter butzleri and a. cryaerophilus are considered potential foodborne pathogens. consumption of arcobacter-contaminated food is regarded the most likely source of human poisoning. we investigated the prevalence and antimicrobial resistance of arcobacter isolates in 210 retail meat samples. seventy-nine a. butzleri and 6 a. cryaerophilus were isolated from pork, beef and chicken meat. incidence ofa. butzleri was found to be the highest in chicken meat (83%). less of a. butzleri was isolated ... | 2015 | 25928911 |
| modified isolation method of arcobacter spp. from different environmental and food samples. | this study provides information on the occurrence of arcobacter in several types of water and food products of animal origin in the czech republic. we processed 190 samples using the modified method, and the occurrence of arcobacter spp. was confirmed in 36.8 % of these. this total incidence consisted of arcobacter butzleri (27.3 %), arcobacter cryaerophilus (8.4 %) and arcobacter skirrowii (1.1 %). we newly described the common presence of arcobacter spp. in sewage water in the czech republic t ... | 2015 | 25912846 |
| insights in the pathogenesis and resistance of arcobacter: a review. | arcobacter genus currently comprises 18 recognized species, among which arcobacter butzleri, arcobacter cryaerophilus and arcobacter skirrowii have been associated with human and animal disease. although these organisms, with special emphasis a. butzleri, are emerging as clinical pathogens, several aspects of their epidemiology and virulence are only starting to be clarified. in vitro human and animal cell culture assays have been used to show that several arcobacter species can adhere to and in ... | 2016 | 25806423 |
| genotyping and genetic diversity of arcobacter butzleri and arcobacter cryaerophilus isolated from different sources by using eric-pcr from india. | arcobacters are important zoonotic pathogens and are transmitted through food and water. they are implicated in causing enteritis in animals and humans. among the arcobacter species, a wide genetic diversity has been documented, which reflects continuous evolving nature of these pathogens. | 2014 | 25333916 |
| prevalence and antimicrobial susceptibility of arcobacter species in cow milk, water buffalo milk and fresh village cheese. | in this study, the presence of arcobacter spp. was examined in cow milk (n=50), water buffalo (wb) milk (n=50) and fresh village cheese (n=50) samples. the 16s rdna-rflp method was used for the identification of arcobacter spp. the disc diffusion method was used to investigate the susceptibility of all strains identified to 18 different antimicrobial substances. the most commonly isolated arcobacter species were found to be arcobacter butzleri (38.89%), arcobacter cryaerophilus (22.23%) and arco ... | 2014 | 25064812 |
| arcobacter: comparison of isolation methods, diversity, and potential pathogenic factors in commercially retailed chicken breast meat from costa rica. | arcobacter species have been recognized as potential food- and waterborne pathogens. the lack of standardized isolation methods and the relatively scarce knowledge about their prevalence and distribution as emerging pathogens are due to the limitations in their detection and identification. this study aimed to determine the presence and the identification of arcobacter in chicken breast samples commercially retailed in san josé, costa rica, as well as to describe the adherence and invasive poten ... | 2014 | 24853508 |
| resveratrol against arcobacter butzleri and arcobacter cryaerophilus: activity and effect on cellular functions. | the frequent isolation of arcobacter butzleri and arcobacter cryaerophilus from food samples makes it imperative to search for potential compounds able to inhibit the development of these bacteria. taking this into consideration, this study focuses on the antimicrobial activity of resveratrol and its mechanism of action against a. butzleri and a. cryaerophilus. the activity of resveratrol was assessed by a microdilution method and time-kill curves. resveratrol effect on cellular functions was as ... | 2014 | 24786554 |
| arcobacter spp. isolated from untreated domestic effluent. | arcobacter butzleri and arcobacter cryaerophilus were isolated from samples of raw untreated domestic sewage influent from nine separate wastewater treatment facilities in cheshire, uk. this is the first report of arcobacter spp. from sewage in the uk and suggests that arcobacter spp. may be present in the human community. | 2014 | 24666283 |
| short communication: occurrence of arcobacter species in industrial dairy plants. | the present study investigated the presence of arcobacter spp. in industrial dairy plants. between february and september 2013, pasteurized milk used for cheesemaking, processing and cleaning water, cheese, and environmental samples from different plant sites, including surfaces in contact or not in contact with food, were sampled. a total of 126 samples were analyzed by the cultural method and isolates were identified by multiplex pcr. arcobacter spp. were isolated from 22 of 75 environmental s ... | 2014 | 24534515 |
| molecular diagnosis of arcobacter and campylobacter in diarrhoeal samples among portuguese patients. | the present study was conducted to investigate the prevalence and diversity of arcobacter and campylobacter spp. in 298 stool samples of patients with diarrhoea, collected from 22 portuguese hospitals, between september and november 2012. detection of arcobacter and campylobacter spp. was performed using molecular-based detection techniques, such as real-time fluorescence resonance energy transfer pcr, species-specific pcr, and sequencing of amplified pcr products. overall, 1.3% of the samples w ... | 2014 | 24361090 |
| occurrence of six virulence-associated genes in arcobacter species isolated from various sources in shiraz, southern iran. | in humans, arcobacters are associated with watery diarrhea and septicemia. although, recently, more cases of diarrhea have been caused by arcobacter species, very little is known about its pathogenesis. therefore, the aim of this study was to evaluate the presence of six putative arcobacter virulence genes (cadf, ciab, cj1349, mvin, plda, and tlya), in a set of 113 arcobacter butzleri, 40 arcobacter cryaerophilus, and 15 arcobacter skirrowii isolates that were recovered from various origins. the ... | 2014 | 24201143 |