| further determination of dna relatedness between serogroups and serovars in the family leptospiraceae with a proposal for leptospira alexanderi sp. nov. and four new leptospira genomospecies. | dna relatedness was determined among 303 strains of leptospira and leptonema. included in the analysis were reference strains from 228 well-characterized and recognized serovars. the study included 268 serovars from 29 named and one or more unnamed serogroups. the strains clustered into 17 dna hybridization groups, representing 12 previously described species (292 strains) and five new genomospecies (11 strains). the largest groups included leptospira interrogans (91 strains from 82 serovars), l ... | 1999 | 10319510 |
| the genus leptospira noguchi, 1917; problems of classification and a suggested system based on antigenic analysis. | | 1954 | 13173355 |
| development of species-specific pcr primer sets for the detection of leptospira. | spirochetes of the genus leptospira infect animals and humans and are the causative agents for the emerging infectious disease leptospirosis. rapid and simple assays for the identification of individual leptospira species are currently not available. for identification of individual leptospira species, pcr primers that detect the ompl1 gene sequence for the majority of pathogenic leptospires were developed in this study. the primer pairs detect leptospira interrogans, leptospira borgpetersenii, ... | 2006 | 17020546 |
| isolation of leptospira noguchii from sheep. | the main goal of this study was to obtain new isolates of leptospira spp. from sheep. a total of 10 kidney samples and 44 blood samples were collected from sheep slaughtered in pelotas, southern brazil. one isolate was obtained which was identified by 16s rrna gene sequencing and serogrouping to be leptospira noguchii serogroup autumnalis. microscopic agglutination test (mat) evaluation revealed that 4.5% of the sheep sera reacted against the autumnalis serogroup. this is the first report of iso ... | 2007 | 17222993 |
| molecular typing of leptospira spp. based on putative o-antigen polymerase gene (wzy), the benefit over 16s rrna gene sequence. | molecular typing of leptospiral strains based on variation within putative o-antigen polymerase gene (wzy) was determined among reference strains and those isolated from patients. using the pcr primers designed from the flanking gene of wzy derived from leptospira interrogans serovar copenhageni, all l. interrogans serovars as well as human and rodent leptospiral isolates from thailand could be amplified. the size of pcr product ranged from 1 to 1.5 kb. the limitation of these primer pairs was t ... | 2007 | 17403048 |
| leptospira noguchii and human and animal leptospirosis, southern brazil. | | 2009 | 19331754 |
| outbreak of leptospirosis among adventure race participants in florida, 2005. | on 21 november 2005, a 32-year-old male resident of new york was hospitalized with suspected leptospirosis. he had participated in an endurance-length swamp race on 4-5 november 2005 outside of tampa, florida. | 2010 | 20146629 |
| Monoclonal antibodies against the leptospiral immunoglobulin-like proteins A and B conserved regions. | Leptospirosis is an infectious disease caused by pathogenic spirochetes of the genus Leptospira that affects humans and a wide variety of animals. Recently the genomes of Leptospira interrogans, Leptospira borgpetersenii and Leptospira biflexa species were sequenced allowing the identification of new virulence factors involved in survival and pathogenesis of bacteria. LigA and LigB are surface-exposed bacterial adhesins whose expression is correlated with the virulence of Leptospira strains. In ... | 2011 | 21903270 |
| evolution of the rnase p rna structural domain in leptospira spp. | we have employed the rnase p rna (rpr) gene, which is present as single copy in chromosome i of leptospira spp. to investigate the phylogeny of structural domains present in the rna subunit of the trna processing enzyme, rnase p. rpr gene sequences of 150 strains derived from ncbi database along with sequences determined from 8 reference strains were examined to fathom strain specific structural differences present in leptospiral rpr. sequence variations in the rpr gene impacted on the configura ... | 2014 | 25463388 |
| rapid maldi-tof mass spectrometry identification of leptospira organisms. | leptospirosis is a worldwide deadly zoonotic disease. accurate identification of the causative leptospira spp. spirochetes ascertains the pathogenic status of the isolates, identifies potential source of infection and recognises outbreaks. species identification is currently based on technically demanding, time and resources consuming serological and molecular methods. matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof-ms) recently emerged as a first-line met ... | 2012 | 22386673 |
| usage of a selective media (emjh-staff) in primary culturing of pathogenic leptospires from bovine clinical samples. | isolation of local strains is mandatory for the success of control programs. however, clinical samples are typically contaminated by other bacteria, which impair leptospires growth. the purpose of this study was to evaluate the use of a previously reported emjh-staff media in the recovery of pathogenic leptospires from bovine clinical samples, namely urine (n = 123) and vaginal fluid-vf (n = 102). emjh-staff presented less contamination than emjh (<0·005), which was more evident in vf culture tu ... | 2015 | 26408270 |
| molecular serovar characterization of leptospira isolates from animals and water in colombia. | leptospirosis is a bacterial disease transmitted directly or indirectly from animals to humans that may result in severe hemorrhagic, hepatic/renal and pulmonary disease. there are 20 known leptospira species and hundreds of serovars, some of which belong to different species. it is essential to identify pathogenic leptospira serovars and their potential reservoirs to prepare adequate control strategies. | 2013 | 24652261 |
| diversity of leptospira spp. in rats and environment from urban areas of sarawak, malaysia. | various prevalence studies on leptospira in animals and humans, as well as environmental samples, had been conducted worldwide, including malaysia. however, limited studies have been documented on the presence of pathogenic, intermediate, and saprophytic leptospira in selected animals and environments. this study was therefore conducted to detect leptospira spp. in rats, soil, and water from urban areas of sarawak using the polymerase chain reaction (pcr) method. a total of 107 rats, 292 soil sa ... | 2017 | 28348601 |
| draft genome sequence of brazilian leptospira noguchii serogroup panama strain u73, isolated from cattle. | leptospira noguchii is a current zoonotic pathogen in brazil. here, we report the draft genome sequence of the brazilian l. noguchii serogroup panama strain u73, isolated from asymptomatic cattle urine. | 2015 | 26472831 |
| first isolation of leptospira noguchii serogroups panama and autumnalis from cattle. | prevention and control of leptospirosis are based on the knowledge of locally circulating strains. thus, efforts to obtain local isolates are paramount to the epidemiological understanding of leptospirosis. we report and discuss here the first isolation of members of serogroups autumnalis and panama from cattle, both belonging to leptospira noguchii species. urine samples (n = 167) were collected directly by puncture of the bladder from randomly selected cows from a slaughterhouse in rio de jane ... | 2015 | 25185756 |