| structure of the repeating unit of the o-specific polysaccharide of the lipopolysaccharide of yersinia kristensenii strain 490 (o:12,25). | the o-specific polysaccharide isolated by mild acid degradation of the lipopolysaccharide of y. kristensenii strain 490 (o:12,25) contained d-glucose, 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-galactose, 2-acetamido-2,6-dideoxy-l-galactose, glycerol, and phosphate in the ratios 2:2:1:1:1:1. on the basis of 31p- and 13c-n.m.r. data, methylation analysis, dephosphorylation, solvolysis with anhydrous hydrogen fluoride, and smith degradation, it was concluded that the repeating unit of th ... | 1992 | 1525785 |
| [the structure of a repetitive unit of the glycerolphosphate- containing o-specific polysaccharide chain from yersinia kristensenii strain 103 (0:12,26) lipopolysaccharide]. | mild acid hydrolysis of the lipopolysaccharide from yersinia kristensenii strain 103 (0:12.26) afforded teichoic acid-like polysaccharide. from the results of methylation, dephosphorylation, partial smyth degradation, and 13c and 31p nmr data the structure of the repeating unit of the polysaccharide was deduced as follows: [formula: see text] the structure was confirmed by complete interpretation of polysaccharide 13c nmr spectrum. | 1990 | 1694078 |
| [detection of virulence markers in clinical strains of yersinia]. | the dispersion of plasmid pyv associated virulence markers in 474 yersinia strains isolated from people has been studied. the ability to autoagglutination, calcium dependence of growth and the specific antigens were identified in 157 strains of traditionally pathogenic yersinia enterocolitica serovars 03, 09, yersinia pseudotuberculosis serovar i. they were not found in 223 strains of other 12 serovars of yersinia enterocolitica, in 40 strains of yersinia frederiksenii, yersinia kristensenii, ye ... | 1991 | 1857370 |
| pathogenicity of yersinia kristensenii for mice. | forty-seven strains of yersinia kristensenii from widely differing sources, representing all known o serogroups of this species, were investigated for virulence with a variety of animal and in vitro assays. twenty-four (51%) of the isolates were lethal for mice pretreated with iron dextran. mouse-lethal strains occurred predominantly within o serogroups o:11, o:12,25, and o:16. virulent y. kristensenii strains generally did not express the virulence-associated phenotype (ca2+ dependence and bind ... | 1991 | 1987029 |
| [quantitative and qualitative studies of yersinia species in the waste water of a purification plant]. | during a long term investigation (october 1986 october 1987) yersinia spp. were isolated from sewage in 5 different stages of a sewage treatment plant in kiel (bülk), schleswig-holstein, frg. methods applied were mpn-procedure, cold enrichment in cin-broth and finally incubation on cin-agar (cefsulodin-irgasan-novobiocin). yersinia enterocolitica were specified according to their serotypes (o:3/o:9) and biotypes. results (median/100 ml): sewage influent 90,000 kbe (log 4.95), aerated grit remova ... | 1990 | 2180421 |
| nucleotide sequence of yst, the yersinia enterocolitica gene encoding the heat-stable enterotoxin, and prevalence of the gene among pathogenic and nonpathogenic yersiniae. | the gene encoding the heat-stable enterotoxin (yst) was cloned from the chromosome of yersinia enterocolitica w1024 (serotype o:9), and the nucleotide sequence was determined. the yst gene encodes a 71-amino-acid polypeptide. the c-terminal 30 amino acids of the predicted protein exactly correspond to the amino acid sequence of the toxin extracted from culture supernatants (t. takao, n. tominaga, and y. shimonishi, biochem. biophys. res. commun. 125:845-851, 1984). the n-terminal 18 amino acids ... | 1990 | 2201642 |
| [the occurrence of yersinia enterocolitica in sewage]. | using a modified cold enrichment procedure yersinia spp. were detected in 90.6% out of 32 raw waste water samples obtained within one year from two municipal sewage treatment plants. moreover yersinia were isolated from 50% of 6 effluent samples. altogether 118 yersinia strains were isolated and typed biochemically and serologically. 69 out of these isolates belonged to yersinia enterocolitica, 60 strains to biotype 1, and 9 to biotype 4, serotype 0:3, 8 strains yersinia enterocolitica serotype ... | 1990 | 2220165 |
| mice and moles inhabiting mountainous areas of shimane peninsula as sources of infection with yersinia pseudotuberculosis. | a total of 1,835 yersinia spp. were isolated from 925 (60.5%) of 1,530 wild mice and from 139 (79.9%) of 174 moles living in mountainous areas of eastern shimane prefecture, japan. the yersinia spp. included 1,106 yersinia enterocolitica, 26 y. enterocolitica-like, 176 yersinia mollaretii, 149 yersinia frederiksenii, 70 yersinia intermedia, 231 yersinia kristensenii, 5 yersinia aldovae, and 72 yersinia pseudotuberculosis. human pathogenic y. enterocolitica was not isolated. y. pseudotuberculosis ... | 1990 | 2254420 |
| comparison of enrichment and plating media for isolation of yersinia. | yersinia enterocolitica (serotypes 0:3 or 0:8), yersinia frederiksenii, yersinia kristensenii, or yersinia intermedia along with 10(8) cells of each of three extraneous organisms (escherichia coli, enterobacter aerogenes, pseudomonas fragi), all commonly found on market poultry, were inoculated into five enrichment media followed by streaking onto 11 plating media to determine the most-efficacious combinations for future surveys or assessment studies. for yersinia enterocolitica (0:8), infrequen ... | 1990 | 2356183 |
| relationships between beta-lactamase production and beta-lactam susceptibility of environmental strains of yersinia kristensenii. | strains of yersinia kristensenii display high susceptibility to carbenicillin (mic90 less than 8 micrograms/ml) in comparison with the majority of environmental strains of yersinia closely related to y. enterocolitica which are resistant to this antibiotic (mic90 greater than 256 micrograms/ml). beta-lactamases of 39 strains of y. kristensenii isolated from foods were analysed by isoelectric focusing and gel electrophoresis of ultrasonically disrupted uninduced cultures. beta-lactamase patterns ... | 1989 | 2626595 |
| clonal diversity and relationships among strains of yersinia enterocolitica. | allelic variation in the chromosomal genome of 81 isolates of yersinia enterocolitica and single isolates of yersinia intermedia, yersinia frederiksenii, yersinia mollaretii, and yersinia kristensenii was assessed by analysis of electrophoretically demonstrable polymorphism in 21 genes encoding metabolic enzymes. eighteen distinctive multilocus genotypes (electrophoretic types [ets]) were identified. clustering of the ets from a matrix of pairwise genetic distances, based on the 21 enzyme loci, ... | 1989 | 2687316 |
| occurrence of yersinia spp. in raw beef, pork and chicken. | one hundred and twenty raw ground beef, pork and chicken samples from ten local grocery stores during a one year period were assayed for the presence of yersinia spp., using direct and postenrichment koh treatment. seven thousand and fifty-five different isolates were recovered from 119 (99%) samples of beef and pork and 118 (98%) samples of chicken, including 27 yersinia enterocolitica serotype 03 biotype 3b phage type 2 (4 pork samples), 4 y. enterocolitica serotype 03 biotype 4 phage type 8 ( ... | 1987 | 3113096 |
| yersiniae and iron. a study in host-parasite relationships. | most enterobacteria obtain the iron they require for growth by producing low-molecular-weight high-affinity iron ligands known as siderophores. these substances chelate and solubilize iron making it available to bacteria. the pathogenic yersiniae produce no detectable siderophores; thus, they proliferate poorly or not at all under conditions of iron limitation. most systemic infections with yersinia enterocolitica occur in patients who are overloaded with iron. this may be due to the presence of ... | 1987 | 3311635 |
| in vitro susceptibility of yersinia kristensenii strains to beta-lactam antibiotics. | the beta-lactam antibiotic susceptibility and beta-lactamase activity of 22 yersinia kristensenii strains were determined in order to compare these properties with those reported for y. enterocolitica. carbenicillin, cephaloridine, cefoxitin and cefotaxime were the most active antimicrobial agents tested against y. kristensenii. all strains were resistant to penicillin g, cephalothin, cephapirin and cephalexin. on the other hand, ampicillin and cefazolin activity could be considerated as interme ... | 1986 | 3501272 |
| occurrence of yersinia enterocolitica in wild-living birds and japanese serows. | yersinia spp. were isolated from 34 of 500 birds representing nine species. the highest isolation rate, 5 of 21 (23.8%), was found in blue magpies (cyanopia cyanus), followed by pheasants (phasianus colchicus tohkaidi), 5 of 33 (15.2%); gray starlings (sturnus cineraceus), 6 of 57 (10.5%); tree sparrows (passer montanus), 1 of 14 (7.1%); bulbuls (hypsipetes amaurotis), 4 of 57 (7.0%); crows (corvus levailantii or corvus corone), 7 of 117 (6.0%); eastern turtledoves (streptopelia orientalis), 4 o ... | 1985 | 3977310 |
| haemagglutinating, adhesive and physico-chemical surface properties of different yersinia enterocolitica and yersinia enterocolitica-like bacteria. | fourteen strains of yersinia enterocolitica, two strains of yersinia kristensenii and one strain of yersinia frederiksenii were investigated for haemagglutination, association with cultured cells, motility and physico-chemical surface properties. three of the y. enterocolitica strains, both y. kristensenii and the y. frederiksenii strain caused haemagglutination. y. enterocolitica o-serotype 3 had the greatest tendency to associate with cultured cells. no correlation was seen between association ... | 1983 | 6191517 |
| yersinia spp. in surface water in matsue, japan. | yersinia spp. (741 strains) were recovered from 81% of 48 surface water samples collected over a 12-month period from four rivers in matsue, japan. the precipitation methods with fecl3 or kaolin and the cold enrichment method with peptone-mannitol-phosphate buffer solution were used for recovery. isolates belonged to yersinia enterocolitica (ye) (133 strains), yersinia intermedia (511 strains), yersinia frederiksenii (57 strains), yersinia kristensenii (10 strains) and x2-like organisms (30 stra ... | 1984 | 6475378 |
| isolation of yersinia spp. from bovine feces. | yersinia spp. were sought in 618 fecal samples from cows. four strains of yersinia enterocolitica, serotype o:12,26 (one), o:13,7 (two), and o:18 (one); seven strains of yersinia kristensenii, serotype o:11,24 (five) and o:12,26 (two); and one strain of yersinia pseudotuberculosis serotype iib, were isolated. this is the first time that y. pseudotuberculosis has been isolated from cows in japan, and the isolation of serotype iib of this organism from cows seems to be the first in the world. | 1983 | 6630475 |
| proposed revision of the wauters et al. antigenic scheme for serotyping of yersinia enterocolitica. | the antigenic scheme of wauters and co-workers was applied to serotype a total of 585 strains of yersinia enterocolitica, 43 strains of yersinia kristensenii, 40 strains of yersinia frederiksenii, and 25 strains of yersinia intermedia. the o antigens 11,23; 11,24; 12,25; 12,26; and 28 were exclusively associated with y. kristensenii. o antigen 16 was associated with y. kristensenii and y. frederiksenii. the serogroups o:4,33 and o:17 were only found in y. intermedia, whereas the remaining serogr ... | 1984 | 6746891 |
| new bacteriophage typing system for yersinia enterocolitica, yersinia kristensenii, yersinia frederiksenii, and yersinia intermedia: correlation with serotyping, biotyping, and antibiotic susceptibility. | yersinia enterocolitica is listed as a single species in bergey's manual of determinative bacteriology, but has recently been split into "true" y. enterocolitica, y. kristensenii, y. intermedia, and y. frederiksenii. from 48 bacteriophages isolated from raw sewage, 24 were chosen as being the most useful for differentiating strains within the four yersinia species. the composite set of 24 phages typed 92% of 236 y. enterocolitica strains, 100% of 16 y. kristensenii strains, 97% of 29 y. frederik ... | 1982 | 7076822 |
| rapid isolation of yersinia spp. from feces. | direct plating or cold enrichment or both have been used to isolate yersinia spp. from feces. freeze-shock double enrichment and koh treatment have been recommended for recovery of yersinia enterocolitica from surface waters and food, respectively. these techniques were evaluated as alternatives for rapid recovery of yersinia spp. from feces. stool samples were homogenized in buffered saline and autoclaved. escherichia coli. klebsiella pneumoniae, and pseudomonas aeruginosa were each added to th ... | 1982 | 7076823 |
| structure of the o-specific polysaccharide of the lipopolysaccharide from yersinia kristensenii o:25.35. | mild hydrolysis of the lipopolysaccharide (lps) from yersinia kristensenii serovar o:25.35 with acid afforded the o-specific polysaccharide (ps) which contained d-glucose, d-galactose, 2-acetamido-2,6-dideoxy-l-galactose, 2-acetamido-2-deoxy-d-glucose, glycerol, and phosphate in the ratios 3:1:1:1:1. on the basis of 31p and 13c nmr spectroscopy, hydrolysis, methylation studies, smith degradation, and dephosphorylation, the repeating unit of ps was shown to have the following structure. [formula: ... | 1993 | 7682474 |
| partial purification and characterization of bacteriocin from yersinia kristensenii. | a raw milk bacterial isolate, identified as yersinia kristensenii was found to produce a bacteriocin which was inhibitory to yersinia enterocolitica but not to other selected species of yersinia or gram-negative bacteria. maximum production of bacteriocin was obtained when the organism was grown in shake culture at 28 degrees c. mitomycin c at a concentration of 0.5 micrograms ml-1 induced bacteriocin production. the bacteriocin was partially purified and characterized by ammonium sulphate fract ... | 1995 | 7730201 |
| application of yersinia kristensenii bacteriocin as a specific marker for the rapid identification of suspected isolates of yersinia enterocolitica. | out of 143 suspected foods and clinical isolates of the genus yersinia, 77 isolates were bacteriocin-sensitive and 66 bacteriocin non-sensitive. of the 77 bacteriocin-sensitive isolates, 72 (93.5%) were identified as y. enterocolitica and 5 (6.49%) as y. frederiksenii. out of 66 bacteriocin non-sensitive suspected isolates, 22 (33.3%) were identified as yersinia spp. other than y. enterocolitica and 43 (65.1%) were other gram-negative bacteria with the exception of one (1.5%) isolate identified ... | 1995 | 7766076 |
| evaluation of the pathogenicity and virulence of yersinia species isolated in edo and delta states of nigeria. | sixteen isolates of four species of yersinia comprising five of yersinia enterocolitica and yersinia fredricksenii, four of yersinia intermedia and two of yersinia kristensenii isolated from domestic and wild animals in villages in edo and delta states, nigeria, were evaluated for their pathogenicity using laboratory animal models and virulence characteristics tests which included autoagglutinability, calcium dependency for growth, heat-stable enterotoxin production and conjunctivities in guinea ... | 1993 | 8026357 |
| comparison of siderophore production and utilization in pathogenic and environmental isolates of yersinia enterocolitica. | yersinia enterocolitica strains of serotypes lethal to mice have been reported previously to produce an endogenous siderophore. in this study, an ethyl acetate-extractable siderophore was characterized and given the name yersiniophore. yersiniophore was produced by 16 of 16 human isolates of serotypes o:4, o:4,32, o:8, o:21, and one nonhuman isolate of serotype o:21. it was not produced by isolates of serotype o:3, o:5, or o:9. one strain of yersinia pseudotuberculosis produced yersiniophore, bu ... | 1994 | 8126201 |
| the reduction of nitrous oxide to dinitrogen by escherichia coli. | escherichia coli k12 reduces nitrous oxide stoichiometrically to molecular nitrogen with rates of 1.9 mumol/h x mg protein. the activity is induced by anaerobiosis and nitrate. n2-formation from n2o is inhibited by c2h2 (ki approximately 0.03 mm in the medium) and nitrite (ki = 0.3 mm) but not by azide. a mutant defective in fnr synthesis is unable to reduce n2o to n2. the reaction in the wild type could routinely be followed by gas chromatography and alternatively by mass spectrometry measuring ... | 1993 | 8297209 |
| population genetics of human, animal, and environmental yersinia strains. | multilocus enzyme electrophoresis was used to analyze 244 strains of nine yersinia species isolated from the environment, animals, and humans at 18 genes encoding metabolic enzymes. all 18 enzymes were polymorphic. among the 137 electrophoretic types (ets) distinguished, the mean allelic diversity per locus was 0.531. yersinia frederiksenii ets were divided into three major clusters that were separated by a large genetic distance, and one et was more closely related to yersinia enterocolitica. t ... | 1993 | 8434911 |
| development of a highly specific assay for rapid identification of pathogenic strains of yersinia enterocolitica based on pcr amplification of the yersinia heat-stable enterotoxin gene (yst). | the chromosomal gene yst, which encodes a heat-stable enterotoxin of yersinia enterocolitica, is a useful diagnostic marker because it occurs only in invasive strains of this species. a homologous gene also occurs in some strains of yersinia kristensenii. sequence analysis of the yst genes from two different strains of y. enterocolitica and from y. kristensenii revealed a substantial number of mismatches at the 3' ends of the yst genes of the so-called american and european biotypes of y. entero ... | 1997 | 9163505 |
| the novel heat-stable enterotoxin subtype gene (ystb) of yersinia enterocolitica: nucleotide sequence and distribution of the yst genes. | the gene (ystb) encoding the novel subtype of the heat-stable enterotoxin (y-stb) was cloned from the chromosome of a clinical isolate of yersinia enterocolitica 84-50 (serotype o:5, biotype 1a) and the nucleotide sequence was determined. the ystb contained 216 base pairs that encoded a protein of 71 amino acid residues. the c-terminal 30 residues of the precursor protein exactly corresponded to the amino acid sequence of the y-stb toxin, purified from the culture supernatant of the wild strain. ... | 1997 | 9344780 |
| [new serological variants of yersinia enterocolitica and yersinia kristensenii]. | seven yersinia strains, selected from a number of cultures, not agglutinated by 32 typing sera, were found to have original antigenic features. these strains were given over to the tarasevich state research institute for standardization and control of medical biological preparations and registered under o-factor formulae and numbers: y. kristensenii 165-036, y. enterocolitica 170-037, y. enterocolitica 168-038, y.enterocolitica 167-039, y. enterocolitica 166-040, y. enterocolitica 169-041, y. kr ... | 1997 | 9432835 |
| the role of thiamine in yersinia kristensenii resistance to antibiotics and heavy metals. | the resistance to divalent metal ions, antibiotics and h2o2 was investigated in yersinia kristensenii strains 13, 15, 18 by performing subcultivations with cdso4 (20 and 100 mg/l) in nutrient agar (na) and m9 medium with thiamine. metal resistance of all three strains in na was the same and decreased in the following sequence: ni > zn = co > cd. the chloramphenicol (cmp) resistance ranged between 32 and 256 mg/l and the h2o2 sensitivity was very low or even zero. in the presence of thiamine the ... | 1997 | 9438358 |
| isolation of yersinia from raw meat (pork and chicken) and precooked meat (porcine tongues and sausages) collected from commercial establishments in mexico city. | a total of 160 meat product samples were collected from commercial outlets in mexico city to investigate the presence of different species of yersinia by the 4 degrees c enrichment method after 1, 3, 5, and 7 days of incubation using alkaline treatment and isolating in cefsulodin-irgasan-novobiocin and macconkey agars with tween 80. overall, yersinia spp. were isolated from 27% of the samples analyzed, whereas 40% of the raw and only 13% of the precooked samples were contaminated. although 2,970 ... | 2000 | 10772223 |
| clinical features of patients with novel yersinia species. | our purpose was to describe the clinical features of patients with novel yersinia species. between 1985 and 1999; 194 patients had yersinia species isolated from stool specimens, 38 (20%) had non-yersinia enterocolitica species; 12 (32%) had yersinia intermedia, 7 (18%) yersinia fredericksenii, 3 (8%) yersinia kristensenii, and the remaining 16 (42%) were unclassified non-yersinia enterocolitica species. the most common presenting symptom was diarrhea alone in 10 (26.3%) patients. symptoms persi ... | 2002 | 12498305 |
| yersinia aleksiciae sp. nov. | yersinia kristensenii consists of phenotypically heterogeneous strains. this is reflected by the existence of strains with various multilocus enzyme electrophoresis and 16s rrna gene sequence types. strains originally phenotyped as members of y. kristensenii were studied using 16s rrna gene sequencing, dna-dna hybridization, determination of the dna base composition and various phenotypic tests. the results were compared to those of yersinia type strains. based on levels of dna-dna relatedness, ... | 2005 | 15774670 |
| use of a culture-independent molecular method to study the ecology of yersinia spp. in food. | a culture-independent method for the direct detection in food of yersinia spp. was developed in this study. it is based on the amplification of a 359 bp pcr product from the rna polymerase beta-subunit gene (rpob) and subsequent analysis by denaturing gradient gel electrophoresis (dgge). direct detection of yersinia spp. by pcr-dgge was carried out in ready-to-eat vegetables and the results compared with the results of the traditional, culture-dependent method. the dgge profiles were determined ... | 2005 | 16085330 |
| the yersinia kristensenii o11 o-antigen gene cluster was acquired by lateral gene transfer and incorporated at a novel chromosomal locus. | we have sequenced the o-antigen gene clusters for the escherichia coli o98 and yersinia kristensenii o11 o antigens. the basic structures of these o antigens are identical, and the sequence data indicate that y. kristensenii o11 gained its o-antigen gene cluster by lateral gene transfer (lgt). escherichia coli o98 has a typical o-antigen gene cluster between galf and gnd as is usual in e. coli. however, the o-antigen gene cluster of y. kristensenii o11 is not located at the traditional yersinia ... | 2007 | 17400574 |
| prevalence of gastrointestinal bacterial pathogens in a population of zoo animals. | faecal prevalence of gastrointestinal bacterial pathogens, including campylobacter, escherichia coli o157:h7, salmonella, shigella, yersinia, as well as arcobacter, were examined in 317 faecal specimens from 44 animal species in belfast zoological gardens, during july-september 2006. thermophilic campylobacters including campylobacter jejuni, campylobacter coli and campylobacter lari, were the most frequently isolated pathogens, where members of this genus were isolated from 11 animal species (1 ... | 2008 | 18331520 |
| scaffolding and validation of bacterial genome assemblies using optical restriction maps. | new, high-throughput sequencing technologies have made it feasible to cheaply generate vast amounts of sequence information from a genome of interest. the computational reconstruction of the complete sequence of a genome is complicated by specific features of these new sequencing technologies, such as the short length of the sequencing reads and absence of mate-pair information. in this article we propose methods to overcome such limitations by incorporating information from optical restriction ... | 2008 | 18356192 |
| production of biogenic amines by lactic acid bacteria and enterobacteria isolated from fresh pork sausages packaged in different atmospheres and kept under refrigeration. | the occurrence of in vitro amino acid activity in bacterial strains associated with fresh pork sausages packaged in different atmospheres and kept in refrigeration was studied. the presence of biogenic amines in decarboxylase broth was confirmed by ion-exchange chromatography and by the presence of the corresponding decarboxylase genes by pcr. from the 93 lactic acid bacteria and 100 enterobacteria strains analysed, the decarboxylase medium underestimates the number of biogenic amine-producer st ... | 2011 | 21316866 |
| n-glycosylation improves the pepsin resistance of histidine acid phosphatase phytases by enhancing their stability at acidic phs and reducing pepsin's accessibility to its cleavage sites. | n-glycosylation can modulate enzyme structure and function. in this study, we identified two pepsin-resistant histidine acid phosphatase (hap) phytases from yersinia kristensenii (ykappa) and yersinia rohdei (yrappa), each having an n-glycosylation motif, and one pepsin-sensitive hap phytase from yersinia enterocolitica (yeappa) that lacked an n-glycosylation site. site-directed mutagenesis was employed to construct mutants by altering the n-glycosylation status of each enzyme, and the mutant an ... | 2016 | 26637601 |
| novel colicin fy of yersinia frederiksenii inhibits pathogenic yersinia strains via yiur-mediated reception, tonb import, and cell membrane pore formation. | a novel colicin type, designated colicin fy, was found to be encoded and produced by the strain yersinia frederiksenii y27601. colicin fy was active against both pathogenic and nonpathogenic strains of the genus yersinia. plasmid yf27601 (5,574 bp) of y. frederiksenii y27601 was completely sequenced. the colicin fy activity gene (cfya) and the colicin fy immunity gene (cfyi) were identified. the deduced amino acid sequence of colicin fy was very similar in its c-terminal pore-forming domain to c ... | 2012 | 22343298 |
| degradation of phytate by the 6-phytase from hafnia alvei: a combined structural and solution study. | phytases hydrolyse phytate (myo-inositol hexakisphosphate), the principal form of phosphate stored in plant seeds to produce phosphate and lower phosphorylated myo-inositols. they are used extensively in the feed industry, and have been characterised biochemically and structurally with a number of structures in the pdb. they are divided into four distinct families: histidine acid phosphatases (hap), β-propeller phytases, cysteine phosphatases and purple acid phosphatases and also split into thre ... | 2013 | 23741456 |
| adhesion to brown trout skin mucus, antagonism against cyst adhesion and pathogenicity to rainbow trout of some inhibitory bacteria against saprolegnia parasitica . | biological control of saprolegniosis with bacteria might be an alternative to the use of chemical compounds. among criteria for the selection of such bacteria are their absence of pathogenicity to fish and their ability to prevent adhesion of the pathogen to the skin mucus. the pathogenicity to rainbow trout of 21 bacterial isolates with in vitro inhibitory activity against saprolegnia parasitica was studied. fifteen of the isolates, identified as aeromonas sobria, pantoea agglomerans, pseudomon ... | 2013 | 23670078 |
| engineering the residual side chains of hap phytases to improve their pepsin resistance and catalytic efficiency. | strong resistance to proteolytic attack is important for feed enzymes. here, we selected three predicted pepsin cleavage sites, l99, l162, and e230 (numbering from the initiator m of premature proteins), in pepsin-sensitive hap phytases ykappa from yersinia kristensenii and yeappa from y. enterocolitica, which corresponded to l99, v162, and d230 in pepsin-resistant yrappa from y. rohdei. we constructed mutants with different side chain structures at these sites using site-directed mutagenesis an ... | 2017 | 28186144 |
| analysis of microbiota on abalone (haliotis discus hannai) in south korea for improved product management. | abalone is a popular seafood in south korea; however, because it contains various microorganisms, its ingestion can cause food poisoning. therefore, analysis of the microbiota on abalone can improve understanding of outbreaks and causes of food poisoning and help to better manage seafood products. in this study, we collected a total of 40 abalones from four different regions in march and july, which are known as the maximum abalone production areas in korea. the microbiota were analyzed using hi ... | 2016 | 27371902 |
| improved model systems for bacterial membranes from differing species: the importance of varying composition in pe/pg/cardiolipin ternary mixtures. | steady-state fluorescence anisotropy and dynamic light scattering (dls) were used to determine the thermotropic properties of lipid systems that act as models for bacterial membranes of yersinia kristensenii and proteus mirabilis. lipid proportions of pe:pg:cl of 0.60:0.20:0.20 and 0.80:0.15:0.05, were used in order to mimic these two membranes respectively. we observed that the introduction of cardiolipin (cl) as a third lipid component of any pe:pg mixture, changes the system's properties cons ... | 2012 | 22830986 |
| yersinia spp. in wild rodents and shrews in finland. | yersinia enterocolitica and yersinia pseudotuberculosis are important zoonotic bacteria causing human enteric yersiniosis commonly reported in europe. all y. pseudotuberculosis strains are considered pathogenic, while y. enterocolitica include both pathogenic and nonpathogenic strains which can be divided into six biotypes (1a, 1b, and 2-5) and about 30 serotypes. the most common types causing yersiniosis in europe are y. enterocolitica bioserotypes 4/o:3 and 2/o:9. strains belonging to biotype ... | 2017 | 28332937 |
| yersinia spp. identification using copy diversity in the chromosomal 16s rrna gene sequence. | api 20e strip test, the standard for enterobacteriaceae identification, is not sufficient to discriminate some yersinia species for some unstable biochemical reactions and the same biochemical profile presented in some species, e.g. yersinia ferderiksenii and yersinia intermedia, which need a variety of molecular biology methods as auxiliaries for identification. the 16s rrna gene is considered a valuable tool for assigning bacterial strains to species. however, the resolution of the 16s rrna ge ... | 2016 | 26808495 |
| prevalence, characterization, and antimicrobial resistance of yersinia species and yersinia enterocolitica isolated from raw milk in farm bulk tanks. | the aims of this study were to investigate the prevalence and to characterize and determine the antibiotic resistance of yersinia spp. isolates from raw milk. from september 2008 to august 2010, 446 raw milk samples were obtained from farm bulk milk tanks in varamin, iran. yersinia spp. were detected in 29 (6.5%) samples, out of which 23 (79.3%), 5 (17.2%), and 1 (3.4%) were isolated from cow, sheep, and goat raw milk, respectively. the most common species isolated was yersinia enterocolitica (6 ... | 2015 | 25497824 |
| draft genome sequences of yersinia frederiksenii, yersinia intermedia, and yersinia kristensenii strains from brazil. | yersinia enterocolitica-like strains are usually understudied. in this work, we reported the draft genome sequences of two yersinia frederiksenii, two yersinia intermedia, and two yersinia kristensenii strains isolated from humans, animals, food, and the environment in brazil. these draft genomes will provide better molecular characterizations of these species. | 2017 | 28798182 |
| the cdi toxin of yersinia kristensenii is a novel bacterial member of the rnase a superfamily. | contact-dependent growth inhibition (cdi) is an important mechanism of inter-bacterial competition found in many gram-negative pathogens. cdi+ cells express cell-surface cdia proteins that bind neighboring bacteria and deliver c-terminal toxin domains (cdia-ct) to inhibit target-cell growth. cdi+ bacteria also produce cdii immunity proteins, which specifically neutralize cognate cdia-ct toxins to prevent self-inhibition. here, we present the crystal structure of the cdia-ct/cdiiykris complex fro ... | 2017 | 28398546 |