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fatal pneumonia due to serratia proteamaculans subsp. quinovora.serratia proteamaculans subsp. quinovora was isolated from several samples (blood cultures, tracheal aspirates, pleural effusion) from a patient with pneumonia. this is the first clinical isolate and the first documented human infection caused by this organism.19938432835
plasmid-located pathogenicity determinants of serratia entomophila, the causal agent of amber disease of grass grub, show similarity to the insecticidal toxins of photorhabdus luminescens.serratia entomophila and serratia proteamaculans cause amber disease in the grass grub costelytra zealandica (coleoptera: scarabaeidae), an important pasture pest in new zealand. larval disease symptoms include cessation of feeding, clearance of the gut, amber coloration, and eventual death. a 115-kb plasmid, padap, identified in s. entomophila is required for disease causation and, when introduced into escherichia coli, enables that organism to cause amber disease. a 23-kb fragment of padap tha ...200010960097
characterization of serratia isolates from soil, ecological implications and transfer of serratia proteamaculans subsp. quinovora grimont et al. 1983 to serratia quinivorans corrig., sp. nov.eleven strains of serratia were isolated from different soils and the guts of invertebrates and characterized by their sensitivity to eight indigenous bacteriophages. they were also classified according to bacteriocin production and sensitivity, biolog plate and api 20e strip profiles and 16s rrna sequence information. one strain was thus identified as serratia plymuthica, another as serratia fonticola. the remaining strains were shown to be closely related to serratia proteamaculans subsp. quin ...200212508898
quorum-sensing-directed protein expression in serratia proteamaculans b5a.n-acyl-l-homoserine-lactone-producing serratia species are frequently encountered in spoiling foods of vegetable and protein origin. the role of quorum sensing in the food spoiling properties of these bacteria is currently being investigated. a set of luxr luxi homologous genes encoding a putative quorum sensor was identified in the n-(3-oxo-hexanoyl)-l-homoserine lactone (3-oxo-c6-hsl)-producing serratia proteamaculans strain b5a. the 3-oxo-c6-hsl synthase spri showed 79 % similarity with esai ...200312624209
biotransformation of adenine and cytokinins by the rhizobacterium serratia proteamaculans.approximately 60,000 microorganisms from saskatchewan soil were screened for growth on the cytokinin n6-benzyladenine (ba) as c source. a single isolate, identified as serratia proteamaculans, grew well on ba. the culture filtrates from s. proteamaculans were screened using reversed phase high performance liquid chromatography (rp-hplc) for the presence of secondary metabolites. the analysis revealed a major metabolite and its chemical structure was deduced as 8-hydroxy-n6-benzyladenine (8-ohba) ...200616860349
characterization of the zinc-containing metalloprotease encoded by zpx and development of a species-specific detection method for enterobacter sakazakii.enterobacter sakazakii causes a severe form of neonatal meningitis that occurs as sporadic cases as well as outbreaks. the disease has been epidemiologically associated with consumption of reconstituted, dried infant formulas. very little information is available regarding pathogenicity of the organism and production of virulence factors. clinical and environmental strains were screened for production of factors which have activity against chinese hamster ovary (cho) cells in tissue culture. pol ...200717483271
genome survey and characterization of endophytic bacteria exhibiting a beneficial effect on growth and development of poplar trees.the association of endophytic bacteria with their plant hosts has a beneficial effect for many different plant species. our goal is to identify endophytic bacteria that improve the biomass production and the carbon sequestration potential of poplar trees (populus spp.) when grown in marginal soil and to gain an insight in the mechanisms underlying plant growth promotion. members of the gammaproteobacteria dominated a collection of 78 bacterial endophytes isolated from poplar and willow trees. as ...200919060168
comparative effectiveness of pseudomonas and serratia sp. containing acc-deaminase for improving growth and yield of wheat (triticum aestivum l.) under salt-stressed conditions.ethylene synthesis is accelerated in response to various environmental stresses like salinity. ten rhizobacterial strains isolated from wheat rhizosphere taken from different salt affected areas were screened for growth promotion of wheat under axenic conditions at 1, 5, 10 and 15 ds m(-1). three strains, i.e., pseudomonas putida (n21), pseudomonas aeruginosa (n39) and serratia proteamaculans (m35) showing promising performance under axenic conditions were selected for a pot trial at 1.63 (origi ...200919255743
oligopeptidase b from serratia proteamaculans. i. determination of primary structure, isolation, and purification of wild-type and recombinant enzyme variants.a novel trypsin-like protease (psp) from the psychrotolerant gram-negative microorganism serratia proteamaculans was purified by ion-exchange chromatography on q-sepharose and affinity chromatography on immobilized basic pancreatic trypsin inhibitor (bpti-sepharose). psp formed a tight complex with groel chaperonin. a method for dissociating the groel-psp complex was developed. electrophoretically homogeneous psp had molecular mass of 78 kda; the n-terminal amino acid sequence 1-10 was determine ...200919916930
[antibacterial effects of silver ions: effect on gram-negative bacteria growth and biofilm formation].minimal inhibiting agno3 concentration (mics) in the gram-negative bacteria escherichia coli k12, serratia proteamaculans 94, and serratia liquefaciens mg1 were found to be on the average within the range of 0.075-0.3 microg/ml, and for pseudomonas aeruginosa pao1 and p. chlororaphis 449, 0.15-0.3 microg/ml. biofilm formation in escherichia coli ab1157 and s. proteamaculans 94 was completely inhibited at an agno3 concentration of 0.3 microg/ml, and in pseudomonas aeruginosa pao1, at 0.6 microg/m ...200920017360
production, purification and characterization of serraticin a, a novel cold-active antimicrobial produced by serratia proteamaculans 136.this study focuses on the production, purification and characterization of serraticin a, a novel cold-active antimicrobial produced by serratia proteamaculans 136.201020408913
gene cloning, expression, and characterization of a new carboxylesterase from serratia sp. ses-01: comparison with escherichia coli biohe enzyme.the carboxylesterase-encoding gene (biohs) of a newly isolated strain, serratia sp. ses-01, was cloned from the genomic dna library by detecting formation of transparent halo around the colony on lb-tributyrin agar plates. the amino acid sequence of biohs was highly similar to the members of the bioh enzyme family involved in the biotin biosynthetic pathway; it showed the highest similarity (91%) with that of serratia proteamaculans. to compare biohs with other bioh enzymes, the relatively well- ...200919307763
cold-adapted microorganisms as a source of new antimicrobials.thirty out of 8,000 different colony morphotypes isolated from soil samples of isla de los estados were selected based on their ability to produce antimicrobials. the significant influence of culture media and incubation temperature on antimicrobial production was proved, being lb medium and 8 degrees c the conditions of choice. most of the psychrotolerant isolates were phylogenetically related to serratia proteamaculans (96.4-97.9%) while the psychrophilic isolated 8h1 was closely related to ps ...200919015813
substrate specificity of a recombinant d-lyxose isomerase from serratia proteamaculans that produces d-lyxose and d-mannose.characterization of substrate specificity of a d-lyxose isomerase from serratia proteamaculans and application of the enzyme in the production of d-lyxose and d-mannose.201020695994
[isolation and properties of cysteine protease from serratia proteamaculans 94].a new cysteine protease (spcp) with a molecular mass of about 50 kda and optimal functioning at ph 8.0 was isolated from the culture medium of a serratia proteamaculans 94 psychrotolerant strain using affinity and gel permeation chromatography. the enzyme n terminal amino acid sequence (spveeaegdgivldv-) exhibits a reliable similarity to n terminal sequences of gingipains r, cysteine proteases from polphyromonas gingivalis. unlike gingipains r, spcp displays a double substrate specificity and cl ...200818672676
burkholderia type vi secretion systems have distinct roles in eukaryotic and bacterial cell interactions.bacteria that live in the environment have evolved pathways specialized to defend against eukaryotic organisms or other bacteria. in this manuscript, we systematically examined the role of the five type vi secretion systems (t6sss) of burkholderia thailandensis (b. thai) in eukaryotic and bacterial cell interactions. consistent with phylogenetic analyses comparing the distribution of the b. thai t6sss with well-characterized bacterial and eukaryotic cell-targeting t6sss, we found that t6ss-5 pla ...201020865170
serratia entomophila inoculation causes a defect in exocytosis in costelytra zealandica larvae.rapid elimination of midgut luminal proteinase activity and gut clearance are the two major symptoms of amber disease in costelytra zealandica larvae because of the three-subunit protein toxin complex produced in serratia entomophila and serratia proteamaculans. quantitative pcr analysis of mrna from the major serine proteinase gene families showed that loss of proteinase activity did not result from transcriptional downregulation. unexpectedly, protein levels and rates of protein synthesis incr ...200818651919
characterization of the enterobacteriaceae community that developed during storage of minced beef under aerobic or modified atmosphere packaging conditions.the whole cell protein and macrorestriction analysis of dna of enterobacteriaceae isolates recovered from minced beef stored at 0, 5, 10 and 15 °c aerobically and under modified atmosphere packaging consisting of 40% co(2)-30% o(2)-30% n(2) in the presence (map+) and absence (map-) of oregano essential oil were studied. sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page) profiles obtained from whole cell protein analysis of the enterobacteriaceae isolates revealed seven groups. ...201021216482
growth of salmonella enteritidis and salmonella typhimurium in the presence of quorum sensing signalling compounds produced by spoilage and pathogenic bacteria.the effect of acylated homoserine lactones (ahls) and autoinducer-2 (ai-2) signalling compounds present in the cell-free culture supernatants (cfs), of pseudomonas aeruginosa, yersinia enterocolitica-like gte 112, serratia proteamaculans 00612, y. enterocolitica city650 and y. enterocolitica city844, on the growth of two salmonella enteritidis and two s. typhimurium strains was assessed though monitoring of changes in conductance of the medium. detection times (t(det)), area and slope of conduct ...201121569946
induced expression of the serratia entomophila sep proteins shows activity towards the larvae of the new zealand grass grub costelytra zealandica.serratia entomophila and serratia proteamaculans cause amber disease of the grass grub costelytra zealandica (coleoptera: scarabaeidae). three genes required for virulence, sepabc, are located on a large plasmid, padap. the translated products of the sep genes are members of the toxin complex (tc) family of insecticidal toxins that reside in the genomes of some enterobacteriaceae. each of the sep genes was placed either singly or as various combinations under the control of an inducible arabinos ...200717714480
psychrophilic trypsin-type protease from serratia proteamaculans.a preparative method for purification of a novel protease from the psychrotolerant gram-negative microorganism serratia proteamaculans (psp) was developed using affinity chromatography on bpti-sepharose. it yielded electrophoretically homogeneous psp preparation of 60 kd. the psp properties (temperature and ph stability, high catalytic efficiency) indicate that this enzyme can be defined as a psychrophilic protease. inhibitory analysis together with substrate specificity indicates that the studi ...200616732738
functional characterization and biological significance of yersinia pestis lipopolysaccharide biosynthesis genes.in silico analysis of available bacterial genomes revealed the phylogenetic proximity levels of enzymes responsible for biosynthesis of lipopolysaccharide (lps) of yersinia pestis, the cause of plague, to homologous proteins of closely related yersinia spp. and some other bacteria (serratia proteamaculans, erwinia carotovora, burkholderia dolosa, photorhabdus luminescens and others). isogenic y. pestis mutants with single or double mutations in 14 genes of lps biosynthetic pathways were construc ...201121999543
predominant enterobacteria on modified-atmosphere packaged meat and poultry.enterobacteria on modified-atmosphere (ma) packaged meat (n = 54) and poultry (n = 32) products were enumerated, and 899 isolates were picked and ribotyped. for identification, 16s rrna genes of representative strains were sequenced and analyzed. altogether 54 (60%) of the samples contained enterobacteria >10(4) cfu/g. in 34% of the poultry samples, enterobacteria counts were >10(6) cfu/g suggesting that enterobacteria may contribute to spoilage of ma packaged poultry. the enterobacteria identif ...201223541191
cloning, sequencing, expression, and characterization of protealysin, a novel neutral proteinase from serratia proteamaculans representing a new group of thermolysin-like proteases with short n-terminal region of precursor.the gene of serratia proteamaculans proteinase, protealysin, was cloned, sequenced, and expressed in escherichia coli. the gene encoded a precursor of 341 amino acids (aas) with a significant homology to thermolysin-like proteinases (tlps). the molecular weight of the purified mature active recombinant protein was 32 kda, the n-terminal amino acid sequence was aktstggevi. the optimum ph for azocasein hydrolysis by protealysin was seven and it was completely inhibited by o-phenanthroline. the enz ...200616442309
peripheral sequences of the serratia entomophila padap virulence-associated region.some strains of the enterobacteriaceae serratia entomophila and serratia proteamaculans cause amber disease in the grass grub, costelytra zealandica (coleoptera: scarabaeidae), an important pasture pest in new zealand. the genes responsible for this disease reside on a large, 155-kb plasmid designated amber disease-associated plasmid (padap). herein, we report the dna sequencing of approximately 50 kb upstream and 10 kb downstream of the virulence-encoding region. based on similarity with protei ...200314597010
an inducible activator produced by a serratia proteamaculans strain and its soybean growth-promoting activity under greenhouse conditions.serratia proteamaculans 1-102 (1-102) promotes soybean-bradyrhizobia nodulation and growth, but the mechanism is unknown. after adding isoflavonoid inducers to 1-102 culture, an active peak with a retention time of about 105 min in the hplc fractionation was isolated using a bioassay based on the stimulation of soybean seed germination. the plant growth-promoting activity of this material was compared with 1-102 culture (cells) and supernatant under greenhouse conditions. the activator was appli ...200212021297
pathobiology of amber disease, caused by serratia spp., in the new zealand grass grub, costelytra zealandica.amber disease in the new zealand grass grub (costelytra zealandica) is caused by some strains of serratia entomophila or serratia proteamaculans (enterobacteriaceae). when treated with pathogenic isolates, larvae ceased feeding within 48 h, developed an amber coloration after 72 h, and entered a long chronic phase without feeding. an acute dose of 2-4 x 10(4) pathogenic bacteria was sufficient to produce disease in 50% of treated larvae. time to death was directly related to temperature. at 15 d ...200112009805
expression, purification and characterization of an endoglucanase from serratia proteamaculans cdbb-1961, isolated from the gut of dendroctonus adjunctus (coleoptera: scolytinae).serratia proteamaculans cdbb-1961, a gut symbiont from the roundheaded pine beetle dendroctonus adjunctus, displayed strong cellulolytic activity on agar-plates with carboxymethyl cellulose (cmc) as carbon source. automatic genome annotation of s. proteamaculans made possible the identification of a single endoglucanase encoding gene, designated spr cel8a. the predicted protein, named spr cel8a shows high similarity (59-94 %) to endo-1,4-β-d-glucanases (ec 3.2.1.4) from the glycoside hydrolase f ...201627576896
methods for detecting acylated homoserine lactones produced by gram-negative bacteria and their application in studies of ahl-production kinetics.in the process of evaluating the role of acylated homoserine lactones (ahls) in food-spoiling gram-negative bacteria, we have combined a range of bacterial ahl monitor systems to determine the ahl-profile and the kinetics of ahl-production. ahl production from 148 strains of enterobacteriaceae isolated from foods was tested using escherichia coli psb403 (luxr), agrobacterium tumefaciens a136 (trar) and both induction and inhibition of chromobacterium violaceum cv026 (cvir). all strains except on ...200111240047
sequence of a lipase-encoding gene isolated from serratia proteamaculans 8805142.a lipase-encoding gene was isolated from a genomic library of the insect pathogen serratia proteamaculans 8805412. the gene encodes a 255-amino-acid polypeptide which has extensive homology to the extracellular phospholipase a1 from s. liquefaciens.19948163166
characterization of the biosynthetic operon for the antibacterial peptide herbicolin in pantoea vagans biocontrol strain c9-1 and incidence in pantoea species.pantoea vagans c9-1 is a biocontrol strain that produces at least two antibiotics inhibiting the growth of erwinia amylovora, the causal agent of fire blight disease of pear and apple. one antibiotic, herbicolin i, was purified from culture filtrates of p. vagans c9-1 and determined to be 2-amino-3-(oxirane-2,3-dicarboxamido)-propanoyl-valine, also known as n(ß)-epoxysuccinamoyl-dap-valine. a plasposon library was screened for mutants that had lost the ability to produce herbicolin i. it was sho ...201222504810
characterization of rara, a novel arac family multidrug resistance regulator in klebsiella pneumoniae.transcriptional regulators, such as soxs, rama, mara, and rob, which upregulate the acrab efflux pump, have been shown to be associated with multidrug resistance in clinically relevant gram-negative bacteria. in addition to the multidrug resistance phenotype, these regulators have also been shown to play a role in the cellular metabolism and possibly the virulence potential of microbial cells. as such, the increased expression of these proteins is likely to cause pleiotropic phenotypes. klebsiel ...201222644028
chitin binding proteins act synergistically with chitinases in serratia proteamaculans 568.genome sequence of serratia proteamaculans 568 revealed the presence of three family 33 chitin binding proteins (cbps). the three sp cbps (sp cbp21, sp cbp28 and sp cbp50) were heterologously expressed and purified. sp cbp21 and sp cbp50 showed binding preference to β-chitin, while sp cbp28 did not bind to chitin and cellulose substrates. both sp cbp21 and sp cbp50 were synergistic with four chitinases from s. proteamaculans 568 (sp chia, sp chib, sp chic and sp chid) in degradation of α- and β- ...201222590591
the acetylation of 6'-amino group of amikacin by a new enzyme prepared from serratia sp.it was found that serratia marcescens 43, serratia proteamaculans 48 and serratia sp. 45, all of which were clinically isolated, produced a new type of aminoglycoside acetyltransferase which acetylated amikacin at the 6'-amino group. 1-n-[(s)-3-amino-2-hydroxypropionyl]-gentamicin b (hapa-b, sch 21420) and gentamicin c2 were hardly inactivated by the enzymes and had effective antimicrobial activities against these strains both in vitro and in vivo. this kind of aminoglycoside acetyltransferase s ...19846526736
gallaecimonas pentaromativorans gen. nov., sp. nov., a bacterium carrying 16s rrna gene heterogeneity and able to degrade high-molecular-mass polycyclic aromatic hydrocarbons.a gram-negative, rod-shaped, halotolerant bacterium, designated strain cee_131(t), which degraded high-molecular-mass polycyclic aromatic hydrocarbons of four and five rings, was isolated from intertidal sediment of corcubion ria in cee, a coruña, spain. direct sequencing showed ambiguities and suggested heterogeneity. cloned 16s rrna gene sequence pcr products yielded five different sequences varying at five positions. strain cee_131(t) showed rather distant relationships to its phylogeneticall ...201019654338
a bioinformatics approach to the determination of genes involved in endophytic behavior in burkholderia spp.the vast majority of plants harbor endophytic bacteria that colonize a portion of the plant's interior tissues without harming the plant. like plant pathogens, endophytes gain entry into their plants hosts through various mechanisms. bacterial endophytes display a broad range of symbiotic interactions with their host plants. the molecular bases of these plant-endophyte interactions are currently not fully understood. in the present study, a set of genes possibly responsible for endophytic behavi ...201424513137
phenotypic changes and the fate of digestive enzymes during induction of amber disease in larvae of the new zealand grass grub (costelytra zealandica).amber disease of the new zealand grass grub costelytra zealandica (coleoptera: scarabaeidae) is caused by ingestion of padap plasmid carrying isolates of serratia entomophila or serratia proteamaculans (enterobacteriaceae) and causes infected larvae to cease feeding and clear their midgut to a pale amber colour where midgut serine protease activities are virtually eliminated. using bacterial strains and mutants expressing combinations of the anti-feeding (afp) and gut clearance (sep) gene cluste ...200919465026
processing of protealysin precursor.protealysin, a protease previously described by us in serratia proteamaculans, belongs to the group of thermolysin-like proteases (tlps) that differ from classical tlps by the precursor structural organization. the propeptide of protealysin precursor has no significant structural similarity to the propeptides of most tlps. the functions of protealysin-like precursors and mechanisms of their action remain unclear. we studied the pathway of protealysin precursor processing in vitro using standard ...200919324072
mesophilic and psychrotrophic bacteria from meat and their spoilage potential in vitro and in beef.mesophilic and psychrotrophic populations from refrigerated meat were identified in this study, and the spoilage potential of microbial isolates in packaged beef was evaluated by analyzing the release of volatile organic compounds (voc) by gas chromatography-mass spectrometry (gc/ms). fifty mesophilic and twenty-nine psychrotrophic isolates were analyzed by random amplified polymorphic dna-pcr, and representative strains were identified by 16s rrna gene sequencing. carnobacterium maltaromaticum ...200919201980
serendipitous crystallization and structure determination of cyanase (cyns) from serratia proteamaculans.cyanate hydratase (cyns) catalyzes the decomposition of cyanate and bicarbonate into ammonia and carbon dioxide. here, the serendipitous crystallization of cyns from serratia proteamaculans (spcyns) is reported. spcyns was crystallized as an impurity and its identity was determined using mass-spectrometric analysis. the crystals belonged to space group p1 and diffracted to 2.1 å resolution. the overall structure of spcyns is very similar to a previously determined structure of cyns from escheric ...201525849512
bacterial invasion of eukaryotic cells can be mediated by actin-hydrolysing metalloproteases grimelysin and protealysin.earlier, we have shown that spontaneously isolated non-pathogenic bacteria serratia grimesii and serratia proteamaculans invade eukaryotic cells, provided that they synthesize thermolysin-like metalloproteases ecp32/grimelysin or protealysin characterized by high specificity towards actin. to address the question of whether the proteases are active players in entry of these bacteria into host cells, in this work, human larynx carcinoma hep-2 cells were infected with recombinant escherichia coli ...201120849390
[cloning and characterization of the chloramphenicol resistant gene of serratia marcescens strain kmr-3].chloramphenicol-resistant gene was cloned and analyzed by constructing genomic dna library of serratia marcescens kmr-3. it showed that cloned chloramphenicol-resistant gene encoded a protein product of 397 amino acids. the protein belonged to prk10473 protein, and it showed 92% similarity to drug resistance transporter, bcr/cfla subfamily of serratia proteamaculans 568. regulation elements including promoter, terminator, shine-dalgarno (sd) sequence and transcription start site also were identi ...200818807985
biochemical and genetic characterization of arazyme, an extracellular metalloprotease produced from serratia proteamaculans hy-3.serratia proteamaculans hy-3 isolated from the digestive tract of a spider produces an extracellular protease named arazyme, with an estimated molecular mass of 51.5 kda. the purified enzyme was characterized as having high activities at wide ph and temperature ranges. we further characterized biochemical features of the enzymatic reactions under various reaction conditions. the protease efficiently hydrolyzed a broad range of protein substrates including albumin, keratin, and collagen. the depe ...200718051297
cloning serratia entomophila antifeeding genes--a putative defective prophage active against the grass grub costelytra zealandica.serratia entomophila and serratia proteamaculans (enterobacteriaceae) cause amber disease in the grass grub costelytra zealandica (coleoptera: scarabaeidae), an important pasture pest in new zealand. larval disease symptoms include cessation of feeding, clearance of the gut, amber coloration, and eventual death. a 155-kb plasmid, padap, carries the genes sepa, sepb, and sepc, which are essential for production of amber disease symptoms. transposon insertions in any of the sep genes in padap abol ...200415262948
presence of acylated homoserine lactones (ahls) and ahl-producing bacteria in meat and potential role of ahl in spoilage of meat.quorum-sensing (qs) signals (n-acyl homoserine lactones [ahls]) were extracted and detected from five commercially produced vacuum-packed meat samples. ninety-six ahl-producing bacteria were isolated, and 92 were identified as enterobacteriaceae. hafnia alvei was the most commonly identified ahl-producing bacterium. thin-layer chromatographic profiles of supernatants from six h. alvei isolates and of extracts from spoiling meat revealed that the major ahl species had an r(f) value and shape simi ...200415240313
antibacterial effects of silver nanoparticles on gram-negative bacteria: influence on the growth and biofilms formation, mechanisms of action.antibacterial action of silver nanoparticles (agnp) on gram-negative bacteria (planctonic cells and biofilms) is reported in this study. agnp of 8.3 nm in diameter stabilized by hydrolyzed casein peptides strongly inhibited biofilms formation of escherichia coli ab1157, pseudomonas aeruginosa pao1 and serratia proteamaculans 94 in concentrations of 4-5 μg/ml, 10 μg/ml and 10-20 μg/ml, respectively. the viability of e. coli ab1157 cells in biofilms was considerably reduced by agnp concentrations ...201323006569
evaluation of natural antimicrobials on typical meat spoilage bacteria in vitro and in vacuum-packed pork meat.the aim of this study was to investigate the inhibitory effect of natural antimicrobials on the growth of typical spoilage bacteria from marinated pork. minimum inhibitory concentrations (mic) of thymol, cinnamaldehyde, allyl isothiocyanate, citric acid, ascorbic acid, a rosemary extract, and a grapefruit seed extract against lactobacillus algidus, leuconostoc mesenteroides, leuconostoc carnosum, carnobacterium maltaromaticum, carnobacterium divergens, brochothrix thermosphacta, and serratia pro ...201020492248
bacterial chitin binding proteins show differential substrate binding and synergy with chitinases.glycosyl hydrolase (gh) family 18 chitinases (chi) and family 33 chitin binding proteins (cbps) from bacillus thuringiensis serovar kurstaki (btchi and btcbp), b. licheniformis dsm13 (blichi and blicbp) and serratia proteamaculans 568 (spchib and spcbp21) were used to study the efficiency and synergistic action of btchi, blichi and spchib individually with btcbp, blicbp or spcbp21. chitinase assay revealed that only btchi and spchib showed synergism in hydrolysis of chitin, while there was no in ...201323480960
probing for actinase activity of protealysin.the ability of protealysin, a thermolysin-like metallopeptidase from serratia proteamaculans 94, to cleave actin and matrix metalloprotease mmp2 is reported. in globular actin, protealysin and s. proteamaculans 94 cell extracts are shown to hydrolyze the gly42-val43 peptide bond within the dnase-binding loop and the gly63-ile64 and thr66-ile67 peptide bonds within the nucleotide cleft of the molecule. at enzyme/substrate mass ratio of 1 : 50 and below, a 36 kda-fragment produced by the cleavage ...200919645670
serratia aquatilis sp. nov., isolated from drinking water systems.a cream-white-pigmented, oxidase-negative bacterium (strain 2015-2462-01t), isolated from a drinking water system, was investigated in detail to determine its taxonomic position. cells of the isolate were rod-shaped and stained gram-negative. a comparison of the 16s rrna gene sequence of strain 2015-2462-01t with sequences of the type strains of closely related species of the genus serratia revealed highest similarity to serratia fonticola (98.4 %), serratia proteamaculans (97.8 %), serratia liq ...201626537514
atp modulates the growth of specific microbial strains.the regulatory function of extracellular atp (exatp) in bacteria is unknown, but recent studies have demonstrated exatp induced enhanced secondary metabolite production and morphological differentiation in streptomyces coelicolor. the growth of streptomyces coelicolor, however, was unaffected by exatp, although changes in growth are common phenotypes. to identify bacteria whose growth is altered by exatp, we measured exatp-induced population changes in fast-growing microbes and actinomycetes in ...201120512647
the role of the chi1 gene from the endophytic bacteria serratia proteamaculans 336x in the biological control of wheat take-all.take-all, a disease caused by the fungus gaeumannomyces graminis var. tritici, is the most important root disease of wheat and causes severe yield losses worldwide. using microorganisms as biological agents to control the disease is important because no resistant cultivars or effective chemical fungicides are available. in this study, we tested the biological control capability of a chitinase produced by the endophytic bacterium serratia proteamaculans 336x against wheat take-all. the chitinase ...201425093749
discovery of a bifunctional acyltransferase responsible for ornithine lipid synthesis in serratia proteamaculans.ornithine lipids (ols) are phosphorus-free membrane lipids that can be formed by many bacteria but that are absent from archaea and eukaryotes. a function for ols in stress conditions and in host-bacteria interactions has been shown in some bacteria. some bacterial species have been described that can form ols, but lack the known genes (olsba) involved in its biosynthesis, which implied the existence of a second pathway. here we describe the bifunctional protein olsf from serratia proteamaculans ...201525040623
nucleotide sequence of the serratia entomophila plasmid padap and the serratia proteamaculans pu143 plasmid virulence associated region.some strains of serratia entomophila and s. proteamaculans cause amber disease of the new zealand grass grub costelytra zealandica (coleoptera: scarabaeidae), an important pasture pest in new zealand. the disease determinants of s. entomophila, are encoded on a 153,404-bp plasmid, termed padap for amber disease associated plasmid. the s. proteamaculans strain 143 (sp143) exhibits an unusual pathotype, where only 60-70% of c. zealandica larvae infected with the bacterium succumb to disease. dna s ...201020950642
inhibitory and toxic effects of volatiles emitted by strains of pseudomonas and serratia on growth and survival of selected microorganisms, caenorhabditis elegans, and drosophila melanogaster.in previous research, volatile organic compounds (vocs) emitted by various bacteria into the chemosphere were suggested to play a significant role in the antagonistic interactions between microorganisms occupying the same ecological niche and between bacteria and target eukaryotes. moreover, a number of volatiles released by bacteria were reported to suppress quorum-sensing cell-to-cell communication in bacteria, and to stimulate plant growth. here, volatiles produced by pseudomonas and serratia ...201425006575
transcriptomic changes in the plant pathogenic fungus rhizoctonia solani ag-3 in response to the antagonistic bacteria serratia proteamaculans and serratia plymuthica.improved understanding of bacterial-fungal interactions in the rhizosphere should assist in the successful application of bacteria as biological control agents against fungal pathogens of plants, providing alternatives to chemicals in sustainable agriculture. rhizoctonia solani is an important soil-associated fungal pathogen and its chemical treatment is not feasible or economic. the genomes of the plant-associated bacteria serratia proteamaculans s4 and serratia plymuthica as13 have been sequen ...201526296338
reducing salt in raw pork sausages increases spoilage and correlates with reduced bacterial diversity.raw sausages are perishable foodstuffs; reducing their salt content raises questions about a possible increased spoilage of these products. in this study, we evaluated the influence of salt reduction (from 2.0% to 1.5% [wt/wt]), in combination with two types of packaging (modified atmosphere [50% mix of co2-n2] and vacuum packaging), on the onset of spoilage and on the diversity of spoilage-associated bacteria. after 21 days of storage at 8°c, spoilage was easily observed, characterized by notic ...201627107120
oligopeptidase b from serratia proteamaculans. ii. enzymatic characteristics: substrate analysis, influence of calcium ions, ph and temperature dependences.enzymatic properties of a novel oligopeptidase b from psychrotolerant gram-negative microorganism serratia proteamaculans (psp) were studied. the substrate specificity of psp was analyzed using p-nitroanilide substrates, and the influence of calcium ions on the enzyme activity was studied. hydrolysis of oligopeptides by psp was studied using melittin as the substrate. optimal conditions for the psp activity (ph and temperature) have been established. it was found that psp shares some properties ...201121585325
cloning, sequencing, expression, and characterization of thermostability of oligopeptidase b from serratia proteamaculans, a novel psychrophilic protease.protease from serratia proteamaculans (psp) is the first known psychrophilic oligopeptidase b. the gene of s. proteamaculans 94 oligopeptidase b was cloned, sequenced and expressed in escherichia coli. the unfolding of psp molecule following heat treatment at 37°c by measuring fluorescence spectra was examined in parallel with the residual activity determination. the effect of psp thermostabilization by glycerol at 37-50 °с was revealed. calcium ions and buffer solution of low molarity cause the ...201424184401
filamentous actin is a substrate for protealysin, a metalloprotease of invasive serratia proteamaculans.homologous bacterial metalloproteases ecp32/grimelysin from serratia grimesii and protealysin from serratia proteamaculans are involved in the invasion of the nonpathogenic bacteria in eukaryotic cells and are suggested to translocate into the cytoplasm [bozhokina es et al. (2011) cell biol int35, 111-118]. the proteases have been characterized as actin-hydrolyzing enzymes with a narrow specificity toward intact cell proteins. however, cleavage of filamentous actin (f-actin) (i.e. the main actin ...201122077798
the metabolically active bacterial microbiome of tonsils and mandibular lymph nodes of slaughter pigs.the exploration of microbiomes in lymphatic organs is relevant for basic and applied research into explaining microbial translocation processes and understanding cross-contamination during slaughter. this study aimed to investigate whether metabolically active bacteria (mab) could be detected within tonsils and mandibular lymph nodes (mlns) of pigs. the hypervariable v1-v2 region of the bacterial 16s rrna genes was amplified from cdna from tonsils and mlns of eight clinically healthy slaughter p ...201526696976
non-contiguous finished genome sequence of plant-growth promoting serratia proteamaculans s4.serratia proteamaculans s4 (previously serratia sp. s4), isolated from the rhizosphere of wild equisetum sp., has the ability to stimulate plant growth and to suppress the growth of several soil-borne fungal pathogens of economically important crops. here we present the non-contiguous, finished genome sequence of s. proteamaculans s4, which consists of a 5,324,944 bp circular chromosome and a 129,797 bp circular plasmid. the chromosome contains 5,008 predicted genes while the plasmid comprises 1 ...201324501629
genes essential for amber disease in grass grubs are located on the large plasmid found in serratia entomophila and serratia proteamaculans.the bacteria serratia entomophila and s. proteamaculans cause amber disease in the grass grub, costelytra zealandica (coleoptera: scarabaeidae), an important pasture pest in new zealand. disease symptoms include rapid cessation of feeding and amber coloration of larvae. a 105-kb plasmid (designated padap) has consistently been found only in pathogenic isolates of both species. investigations into the involvement of padap in amber disease have been hindered by the lack of both a selectable marker ...199516535045
kinetics of biofilm formation and desiccation survival of listeria monocytogenes in single and dual species biofilms with pseudomonas fluorescens, serratia proteamaculans or shewanella baltica on food-grade stainless steel surfaces.this study investigated the dynamics of static biofilm formation (100% rh, 15 °c, 48-72 h) and desiccation survival (43% rh, 15 °c, 21 days) of listeria monocytogenes, in dual species biofilms with the common spoilage bacteria, pseudomonas fluorescens, serratia proteamaculans and shewanella baltica, on the surface of food grade stainless steel. the gram-negative bacteria reduced the maximum biofilm population of l. monocytogenes in dual species biofilms and increased its inactivation during desi ...201324102145
truncated variants of serratia proteamaculans oligopeptidase b having different activities.treatment of native psychrophilic oligopeptidase b from serratia proteamaculans (psp, 78 kda) with chymotrypsin (soluble or immobilized on modified porous glass mpg-pa) in the presence of 50% glycerol leads to production of a truncated enzyme form (psp-chtr, ~66 kda), which retains activity toward the low molecular weight substrate of psp, bapna, but in contrast to psp, is active toward the protein substrate azocasein. it has been shown by maldi-tof mass-spectrometry that psp-chtr lacks the n-te ...201526567578
selection of bioprotective cultures for preventing cold-smoked salmon spoilage.biopreservation is a natural technology of food preservation, which consists of inoculating food with microorganisms selected for their antibacterial properties. the objective of this study was to select lactic acid bacteria (lab) to improve the quality of cold-smoked salmon (css). in this work, different strains representative of the 4 dominant species, identified in a previous study by pyrosequencing the 16s rrna gene, were isolated and their spoiling potential in css blocks, sterilized by ion ...201526044337
carnobacterium divergens - a dominating bacterium of pork meat juice.nonspoiled food that nevertheless contains bacterial pathogens constitutes a much more serious health problem than spoiled food, as the consumer is not warned beforehand. however, data on the diversity of bacterial species in meat juice are rare. to study the bacterial load of fresh pork from ten different distributors, we applied a combination of the conventional culture-based and molecular methods for detecting and quantifying the microbial spectrum of fresh pork meat juice samples. altogether ...201222537055
inverse relationship between chitobiase and transglycosylation activities of chitinase-d from serratia proteamaculans revealed by mutational and biophysical analyses.serratia proteamaculans chitinase-d (spchid) has a unique combination of hydrolytic and transglycosylation (tg) activities. the tg activity of spchid can be used for large-scale production of chito-oligosaccharides (chos). the multiple activities (hydrolytic and/or chitobiase activities and tg) of spchid appear to be strongly influenced by the substrate-binding cleft. here, we report the unique property of spchid substrate-binding cleft, wherein, the residues tyr28, val35 and thr36 control chito ...201526493546
production of bioactive chitosan oligosaccharides using the hypertransglycosylating chitinase-d from serratia proteamaculans.the biological activities of chitosan and its oligosaccharides are greatly influenced by properties such as the degree of polymerization (dp), degree of acetylation (da) and pattern of acetylation (pa). here, structurally diverse chitosan oligosaccharides from chitosan polymers (da=35% or 61%) were generated using serratia proteamaculans wild-type chitinase d (spchid) and the w114a mutant which lacks transglycosylase activity. the crude oligosaccharide mixtures and purified fractions with specif ...201526432054
catalytic efficiency of chitinase-d on insoluble chitinous substrates was improved by fusing auxiliary domains.chitin is an abundant renewable polysaccharide, next only to cellulose. chitinases are important for effective utilization of this biopolymer. chitinase d from serratia proteamaculans (spchid) is a single domain chitinase with both hydrolytic and transglycosylation (tg) activities. spchid had less of hydrolytic activity on insoluble polymeric chitin substrates due to the absence of auxiliary binding domains. we improved catalytic efficiency of spchid in degradation of insoluble chitin substrates ...201525615694
mutagenesis and molecular dynamics simulations revealed the chitooligosaccharide entry and exit points for chitinase d from serratia proteamaculans.transglycosylation (tg) activity is a property of glycosyl hydrolases (ghs) with which new glycosidic bonds are introduced between donor and acceptor sugar molecules. this special property of the ghs has potential to generate longer chain chitooligosaccharides (chos) that show elicitor activity in plants. we hypothesize that tg activity could be improved by retaining the substrate for a longer duration in the catalytic site.201424972166
decarboxylase gene expression and cadaverine and putrescine production by serratia proteamaculans in vitro and in beef.studies of the molecular basis of microbial metabolic activities that are important for the changes in food quality are valuable in order to help in understanding the behavior of spoiling bacteria in food. the growth of a psychrotrophic serratia proteamaculans strain was monitored in vitro and in artificially inoculated raw beef. two growth temperatures (25°c and 4°c) were tested in vitro, while growth at 15°c and 4°c was monitored in beef. during growth, the expression of inducible lysine and o ...201323811038
transglycosylation by chitinase d from serratia proteamaculans improved through altered substrate interactions.we describe the improvement of transglycosylation (tg) by chitinase d from serratia proteamaculans (spchid). the spchid produced a smaller quantity of tg products for up to 90 min with 2 mm chitotetraose as the substrate and subsequently produced only hydrolytic products. of the five residues targeted at the catalytic center, e159d resulted in substantial loss of both hydrolytic and tg activities. y160a resulted in a product profile similar to spchid and a rapid turnover of substrate with slight ...201223115231
oligopeptidase b from serratia proteamaculans. iii. inhibition analysis. specific interactions with metalloproteinase inhibitors.inhibition of the novel oligopeptidase b from serratia proteamaculans (psp) by basic pancreatic trypsin inhibitor, zn2+ ions, and o- and m-phenanthroline was investigated. a pronounced effect of calcium ions on the interaction of psp with inhibitors was demonstrated. inversion voltamperometry and atomic absorption spectrometry revealed no zinc ions in the psp molecule. hydrophobic nature of the enzyme inhibition by o- and m-phenanthroline was established.201222803948
comparison of four feed proteases for improvement of nutritive value of poultry feather meal.feed industries are seeking new ways to cope with increased raw material costs, and one approach is to apply enzymatic treatment in the production of feed ingredients from animal by-products. keratinases, a group of proteases, are capable of hydrolyzing keratin-rich material and have been applied in the production of cost-effective feather by-products for use as feed and fertilizers. the current study examined 4 commercial feed proteases from bacillus subtilis, bacillus licheniformis pwd-1, aspe ...201223365376
synthesis of long-chain chitooligosaccharides by a hypertransglycosylating processive endochitinase of serratia proteamaculans 568.we describe the heterologous expression and characterization of a 407-residue single-domain glycosyl hydrolase family 18 chitinase (spchid) from gram-negative serratia proteamaculans 568 that has unprecedented catalytic properties. spchid was optimally active at ph 6.0 and 40 °c, where it showed a k(m) of 83 mg ml(-1), a k(cat) of 3.9 × 10(2) h(-1), and a k(cat)/k(m) of 4.7 h mg(-1) ml(-1) on colloidal chitin. on chitobiose, the k(m), k(cat), and k(cat)/k(m) were 203 μm, 1.3 × 10(2) h(-1), and 0 ...201222685288
identification of oral bacterial dna in synovial fluid of patients with arthritis with native and failed prosthetic joints.we examined the presence of bacterial dna in synovial fluids of native or clinically aseptically failed prosthetic joints from patients having periodontal disease and arthritis to determine whether there is bacterial spread from the oral cavity to the joints.201222426587
structure of chitinase d from serratia proteamaculans reveals the structural basis of its dual action of hydrolysis and transglycosylation.chitinases are known to hydrolyze chitin polymers into smaller chitooligosaccharides. chitinase from bacterium serratia proteamaculans (spchid) is found to exhibit both hydrolysis and transglycosylation activities. spchid belongs to family 18 of glycosyl hydrolases (gh-18). the recombinant spchid was crystallized and its three-dimensional structure was determined at 1.49 å resolution. the structure was refined to an r-factor of 16.2%. spchid consists of 406 amino acid residues. the polypeptide c ...201324380021
hexavalent chromium removal by a novel serratia proteamaculans isolated from the bank of sebou river (morocco).the novel serratia proteamaculans isolated from a chromium-contaminated site was tolerant to a concentration of 500 mg cr(vi)/l. the optimum ph and temperature for reduction of cr(vi) by s. proteamaculans were found to be 7.0 and 30 °c, respectively. the cr(vi) reduction rate decreased with the increase in cr(vi) concentration from 100 to 400 mg/l, suggesting the enzymatic chromium reduction. resting and permeabilised cell assays provided the better evidence that chromate reduction in s. proteam ...201424194414
identification and characterization of an unusual metallo-β-lactamase from serratia proteamaculans.metallo-β-lactamases (mbls) are a family of metalloenzymes that are capable of hydrolyzing β-lactam antibiotics and are an important means by which bacterial pathogens use to inactivate antibiotics. a database search of the available amino acid sequences from serratia proteamaculans indicates the presence of an unusual mbl. a full length amino acid sequence alignment indicates overall homology to b3-type mbls, but also suggests considerable variations in the active site, notably among residues t ...201323982345
multiple chitinases of an endophytic serratia proteamaculans 568 generate chitin oligomers.serratia proteamaculans 568 genome revealed the presence of four family 18 chitinases (sp chia, sp chib, sp chic, and sp chid). heterologous expression and characterization of sp chia, sp chib, and sp chic showed that these enzymes were optimally active at ph 6.0-7.0, and 40°c. the three sp chitinases displayed highest activity/binding to β-chitin and showed broad range of substrate specificities, and released dimer as major end product from oligomeric and polymeric substrates. longer incubation ...201222406064
psychrophile spoilers dominate the bacterial microbiome in musculature samples of slaughter pigs.the aim of this study was to disentangle the microbial diversity on porcine musculature. the hypervariable v1-v2 region of the 16s rrna gene was amplified from dna samples of clinically healthy slaughter pigs (n=8). pyrosequencing yielded 37,000 quality-controlled reads and a diverse microbiome with 54-159 otus per sample was detected. interestingly, 6 out of 8 samples were strongly dominated by 1-2 highly abundant otus (best hits of highly abundant otus: serratia proteamaculans, pseudomonas syr ...201626943946
influence of volatile organic compounds emitted by pseudomonas and serratia strains on agrobacterium tumefaciens biofilms.the ability to form biofilms plays an important role in bacteria-host interactions, including plant pathogenicity. in this work, we investigated the action of volatile organic compounds (vocs) produced by rhizospheric strains of pseudomonas chlororaphis 449, pseudomonas fluorescens b-4117, serratia plymuthica ic1270, as well as serratia proteamaculans strain 94, isolated from spoiled meat, on biofilms formation by three strains of agrobacterium tumefaciens which are causative agents of crown-gal ...201627214244
the spoilage characteristics of brochothrix thermosphacta and two psychrotolerant enterobacteriacae in vacuum packed lamb and the comparison between high and low ph cuts.the spoilage potential of brochothrix thermosphacta, serratia proteamaculans and rahnella aquatilis was investigated in vacuum packaged high (5.9 to 6.4) and low (5.4 to 5.8) ph lamb. vacuum packaged fore shank (m. extensor carpi radialis) and striploins (m. longissimus dorsi) (n=306) inoculated with ~100 cfu of individual bacteria were stored for twelve weeks at temperatures -1.5, 0, 2 and 7°c. spoilage characteristics and bacterial numbers were recorded and analysed in comparison to un-inocula ...201424548927
virulence factors contributing to invasive activities of serratia grimesii and serratia proteamaculans.previously, we have shown that facultative pathogens serratia grimesii and serratia proteamaculans are capable to invade eukaryotic cells provided that they synthesize intracellular metalloprotease grimelysin or protealysin, respectively (bozhokina et al. in cell biol int 35(2):111-118, 2011). noninvasive escherichia coli transformed with grimelysin or protealysin gene became invasive, indicating that the protease is a virulence factor. here we elucidated involvement of other virulence factors i ...201525577256
bacterial communities of fresh goat meat packaged in modified atmosphere.the objective of this work was to study the growth and development of fortuitous flora and food pathogens in fresh goat meat packaged under modified atmospheres containing two different concentrations of co2. meat samples were stored at 10 °c under two different modified-atmosphere packing (map) conditions: treatment a had 45% co2 + 20% o2 + 35% n2 and treatment b had 20% co2 + 55% o2 + 25% n2. during 14 days of storage, counts of each bacterial group and dominant species identification by 16s r ...201728400020
andrimid production at low temperature by a psychrotolerant serratia proteamaculans strain.andrimid, a known non-ribosomal pseudo-peptide antibiotic, was isolated from a psychrotolerant serratia proteamaculans strain. the antibiotic peptide was produced at low temperature (8 °c) in a 7.5 l bioflo 101 bioreactor under batch culture mode. andrimid activity from s. proteamaculans culture was only detected at 25 °c and below and potent antibacterial activity was revealed against both, pathogenic and non-pathogenic bacteria. minimal inhibitory concentration values determined by microdiluti ...201323558460
influence of food preservation parameters and associated microbiota on production rate, profile and stability of acylated homoserine lactones from food-derived enterobacteriaceae.quorum-dependent regulation is mediated by n-acyl-l-homoserine lactones (ahls) in several gram-negative bacteria. the production of ahls has typically been studied using pure bacteria cultures grown in nutrient-rich media at optimal temperature. ahls are produced in several chill-stored foods by gram-negative bacteria participating in spoilage. as part of our investigation of the role of ahls in food quality, we studied the ahl production in two enterobacteriaceae isolated from cold-smoked salmo ...200312781938
draft genome sequence of serratia proteamaculans mfpa44a14-05, a model organism for the study of meat and seafood spoilage.in this study, we present a draft genome sequence of serratia proteamaculans mfpa44a14-05. this strain was isolated from a spoiled organic modified-atmosphere-packed beef carpaccio. the draft genome sequence will contribute to the understanding of the role of the s. proteamaculans species in meat and seafood spoilage.201728596408
enzymatic production of n-acetyl-d-glucosamine from crayfish shell wastes pretreated via high pressure homogenization.this study presents an efficient pretreatment of crayfish shell using high pressure homogenization that enables n-acetyl-d-glucosamine (glcnac) production by chitinase. firstly, the chitinase from serratia proteamaculans nj303 was screened for its ability to degrade crayfish shell and produce glcnac as the sole product. secondly, high pressure homogenization, which caused the crayfish shell to adopt a fluffy netted structure that was characterized by scanning electron microscope (sem), fourier t ...201728578959
activity modulation of the oligopeptidase b from serratia proteamaculans by site-directed mutagenesis of amino acid residues surrounding catalytic triad histidine.oligopeptidase b (opdb; ec 3.4.21.83) is a trypsin-like peptidase belonging to the family of serine prolyl oligopeptidases; two-domain structure of the enzyme includes c-terminal peptidase catalytic domain and n-terminal seven-bladed β-propeller domain. importance of the interface between these domains and particularly of the 5 salt bridges for enzyme activity was established for protozoan opdbs. however, these salt bridges are not conserved in γ -proteobacterial opdbs including the peptidase fr ...201728554571
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