| identification of proteases from periodontopathogenic bacteria as activators of latent human neutrophil and fibroblast-type interstitial collagenases. | activation of latent human fibroblast-type and neutrophil interstitial procollagenases as well as degradation of native type i collagen by supra- and subgingival dental plaque extracts, an 80-kda trypsinlike protease from porphyromas gingivalis (atcc 33277), a 95-kda chymotrypsinlike protease from treponema denticola (atcc 29522), and selected bacterial species commonly isolated in periodontitis was studied. the bacteria included were prevotella intermedia (atcc 25261), prevotella buccae (es 57) ... | 1992 | 1398963 |
| [bacteriological study on juvenile periodontitis]. | the predominant cultivable microflora of 23 pockets in 15 juvenile periodontitis (jp) patients was studied for the first time in china using the current anaerobic methodology. samples were taken with sterile paper points and dispersed on a vortex mixer. then the diluted samples were plated on the non-selective blood agar plates and selective mgb medium which favors the growth of actinobacillus actimycetemcomitans (aa) and incubated in anaerobic chamber for 5 days. from each sample 15 or more iso ... | 1991 | 1676335 |
| comparative in vitro activity of cefpodoxime against anaerobes other than bacteroides fragilis. | to assess the in vitro activity of cefpodoxime against anaerobic respiratory tract and oropharyngeal pathogens 77 strains belonging to 18 gram-negative and 7 gram-positive species were studied by means of agar dilution tests. for comparison cefuroxime, amoxicillin, amoxicillin + clavulanic acid and clindamycin were also tested. cefpodoxime was found to be active at concentrations of less than or equal to 0.125 mg/l against prevotella oralis, prevotella buccalis, prevotella bivia, porphyromonas a ... | 1991 | 1800380 |
| identification, and susceptibility to seven antimicrobial agents, of 61 gram-negative anaerobic rods from periodontal pockets. | sixty-one cultures of gram-negative anaerobic rods were isolated from deep periodontal pockets of patients with rapidly progressive periodontitis. isolates were speciated as bacteroides gingivalis (18 isolates), bacteroides intermedius (8), bacteroides oris (1), bacteroides gracilis (17) and fusobacterium nucleatum (17). their susceptibilities, to seven antimicrobial agents, were determined in vitro using a plate dilution technique. amoxycillin and amoxycillin with clavulanic acid were active ag ... | 1991 | 1901873 |
| multivariate analyses of cellular fatty acids in bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp. | the genera bacteroides, wolinella, and campylobacter contain several similar species that require taxonomic revision. fatty acid profiles of whole bacterial cells have proven useful for taxonomy. in this study, cellular fatty acids from bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp. were identified and quantitated by gas chromatography and gas chromatography-mass spectrometry, and the data were subjected to principal component analyses. bacteroides fragilis, the type s ... | 1991 | 1993755 |
| ingestion of bacteroides buccae, bacteroides oris, porphyromonas gingivalis, and fusobacterium nucleatum by human polymorphonuclear leukocytes in vitro. | the phagocytic ingestion of clinical isolates and reference strains of bacteroides buccae, bacteroides oris, porphyromonas gingivalis and fusobacterium nucleatum by polymorphonuclear leukocytes (pmns) was studied. special attention was focused on the hydrophobicity of the strains. b. buccae strains, less or equally hydrophobic than pmns, were poorly ingested without opsonization. hydrophobic, but not hydrophilic, strains of b. oris and both hydrophilic and hydrophobic p. gingivalis and f. nuclea ... | 1990 | 2082243 |
| newly delineated periodontal pathogens with special reference to selenomonas species. | many new species have been isolated from subgingival periodontal pockets, for example wolinella recta and bacteroides forsythus, reflecting better basic microbiological techniques. other species were created from existing species as a result of better characterization methods, i.e. bacteroides buccae and bacteroides oris. we can recognize different types of periodontal disease and can find differences relating to the progressive compared to inactive lesions. data illustrated in this presentation ... | 1989 | 2661440 |
| use of the rapid-ana system to screen for enzyme activities that differ among species of bile-inhibited bacteroides. | the rapid-ana system (innovative diagnostics systems, inc., atlanta, ga.) was used to test 102 strains of 14 species of phenotypically similar bile-inhibited bacteroides from humans. bacteroides oris, bacteroides veroralis, bacteroides buccalis, bacteroides melaninogenicus, bacteroides loescheii, and bacteroides denticola had very similar enzyme activity profiles. clear differentiation of these six species by the rapid-ana system was not possible, but tests for arginine aminopeptidase and beta-g ... | 1986 | 2871042 |
| emergence and persistence of beta-lactamase-producing bacteria in the oropharynx following penicillin treatment. | the emergence and persistence of aerobic and anaerobic beta-lactamase-producing bacteria (blpb) were investigated in 26 children treated with penicillin for otitis media or pharyngitis and in 28 nontreated control children. beta-lactamase-producers were isolated in three (12%) of the treated children before therapy, in 12 (46%) seven to ten days after completion of therapy, in nine (35%) 40 to 45 days after therapy, and in seven (27%) 85 to 90 days after therapy. these organisms were present in ... | 1988 | 3130087 |
| [frequency of isolation and antibiotic sensitivity of bacteroides related to bacteroides bivius and bacteroides oralis]. | 274 strains of the bacteroides oralis-bivius group are studied: 112 are identified to bacteroides bivius or b. disiens, 73 to bacteroides oralis and 49 to bacteroides oris or bacteroides buccae. these strains are isolated from clinical sample: gynecologic suppurations or respiratory tract infections. the susceptibility of 63 strains to 7 antibiotics were determined. tested antibiotics were: cefalotin, cefoxitine, cefotaxime, mezlocillin, clindamycin, metronidazole and colistine. cefalotine has a ... | 1987 | 3302864 |
| bacteriologic and clinical study of bacteroides oris and bacteroides buccae. | we characterized clinical isolates previously identified in our laboratory as bacteroides ruminicola, the human strains of which are now classified as bacteroides oris and bacteroides buccae. a total of 72 isolates (55 b. buccae isolates and 17 b. oris isolates) recovered over a 10-year period were studied. they were differentiated from each other by special-potency antibiotic disks and the rapid-ana system. the two organisms were associated with a variety of infections, the majority being pleur ... | 1987 | 3571453 |
| taxonomy of anaerobes: present state of the art. | results of nucleic acid studies, which indicate relationships among strains and species more clearly than do usual phenotypic tests, have led to new bacteriologic nomenclature. some major changes in bacteroides include the recognition of three species (bacteroides melaninogenicus, bacteroides denticola, and bacteroides loescheii) formerly grouped in b. melaninogenicus subspecies melaninogenicus; two species (bacteroides intermedius with two closely related homology groups and bacteroides corpori ... | 1984 | 6201990 |
| a retrospective review of cases of anaerobic empyema and update of bacteriology. | we conducted a retrospective study to update the bacteriology of 46 cases of anaerobic empyema that were originally studied between 1976 and 1993 at the wadsworth anaerobic bacteriology clinical research laboratory (los angeles). anaerobic bacteriologic studies were completed for all 46 pleural fluid specimens, and aerobic bacteriologic studies were completed for 41 of these specimens. thirty-seven clinical charts were available for review. a total of 161 anaerobic isolates (3.5 per patient) rep ... | 1995 | 7548560 |
| evidence for the absence of hyaluronidase activity in porphyromonas gingivalis. | the aim of the present study was to evaluate the ability of porphyromonas gingivalis to degrade hyaluronic acid. no hyaluronidase activity was detected using a turbidimetric method, whereas a standard plate assay showed a positive reaction for p. gingivalis. we postulated that the high proteolytic activity of p. gingivalis may account for this observation. a modified plate assay was designed to avoid false-positive reactions caused by proteolytic bacteria. the new assay, based on the formation o ... | 1993 | 8394379 |
| bacteriologic findings in tonsillitis and pericoronitis. | bacteriologic samples from 31 young men were cultured quantitatively for aerobes and anaerobes; these samples included 31 specimens of tonsils (16 infected and 15 healthy), 16 specimens from pericoronal pockets of lower third molars (11 infected and 5 symptom-free), and 6 postoperative specimens from lower-third-molar extraction sockets. anaerobes were isolated more often from infected third molars than from infected tonsils (14.5 isolates vs. 8.4 isolates, respectively; p < .001). infected tons ... | 1996 | 8816129 |
| molecular analysis of microflora associated with dentoalveolar abscesses. | the microflora associated with three dentoalveolar abscesses was determined by cultural and molecular methods. 16s rrna genes were randomly amplified by means of conserved eubacterial primers and cloned. restriction fragment length polymorphism analysis of the clones and amplified genes encoding 16s rrna from the cultured bacteria was used to detect putative unculturable bacteria. clones representative of five predominant groups of uncultured organisms were sequenced. two were identified as porp ... | 1996 | 8904410 |
| inhibition of prevotella and capnocytophaga immunoglobulin a1 proteases by human serum. | oral prevotella and capnocytophaga species, regularly isolated from periodontal pockets and associated with extraoral infections, secret specific immunoglobulin a1 (iga1) proteases cleaving human iga1 in the hinge region into intact fab and fc fragments. to investigate whether these enzymes are subject to inhibition in vivo in humans, we tested 34 sera from periodontally diseased and healthy individuals in an enzyme-linked immunosorbent assay for the presence and titers of inhibition of seven pr ... | 1997 | 9220164 |
| role of human intestinal prevotella oris in hydrolyzing ginseng saponins. | the potential of intestinal bacteria to hydrolyze ginsenoside rb1 to 20-o-beta-d-glucopyranosyl-20(s)-protopanaxadiol (i) was found in 79% of the fecal specimens from 58 human subjects whose age ranged from 1 to 64 years. following a ginsenoside-rb1-hydrolyzing activity assay, prevotella oris strains were then isolated as a major bacterial species possessing the potential. all the intestinal isolates converted ginsenosides rb1 and rd to i, ginsenoside rb2 to 20-o-[alpha-l-arabinopyranosyl(1-->6) ... | 1997 | 9342949 |
| bacteriostatic effects of hyaluronic acid. | this investigation is one of a series of projects seeking to ascertain whether hyaluronic acid (ha) is therapeutically effective in tissue regeneration procedures. the rationale for these investigations is to test the hypothesis that ha can serve as a bioabsorbable carrier for other substrates as well as itself actively promote the regeneration of tissue. | 1999 | 10328647 |
| activities of gemifloxacin (sb 265805, lb20304) compared to those of other oral antimicrobial agents against unusual anaerobes. | the activities of gemifloxacin (sb 265805, lb20304) and comparator agents were determined by an agar dilution method against 419 clinical strains of less-commonly identified species of anaerobes. gemifloxacin was generally more active than trovafloxacin against gram-positive strains by one to two dilutions. peptostreptococci (peptostreptococcus asaccharolyticus, peptostreptococcus magnus, peptostreptococcus micros, and peptostreptococcus prevotii) and porphyromonas spp. (porphyromonas asaccharol ... | 1999 | 10543754 |
| [pathogen spectrum and resistance status of exclusively anaerobic odontogenic infections]. | out of 440 dentogenic pyogenic infections, 171 exclusively caused by anaerobes were investigated to understand the importance of anaerobic bacteria in dental pyogenic processes better. grampositive anaerobic bacteria dominated. the predominant grampositive isolates in monoinfections were peptostreptococci and in the case of mixed infections, strains of the genus eubacterium. strains of prevotella and porphyromonas dominated the gramnegative anaerobic spectra. the resistance to penicillin was ver ... | 2000 | 10900958 |
| antibacterial activity of fifth-generation dentin bonding systems. | past concepts that the pulp does not become infected until an actual carious exposure takes place have been challenged. the antibacterial effects of the dentin bonding systems single bond, prime&bond nt, and excite were evaluated using the bacteria streptococcus mutans atcc 25175, streptococcus intermedius, lactobacillus acidophilus, prevotella oris, prevotella bivia, prevotella denticola, porphyromonas gingivalis, porphyromonas endodontalis, and clostridium ramosum with a disk diffusion method. ... | 2001 | 11771577 |
| [study of bacteriology on local application of tinidazole stilus in treatment for periodontitis]. | the study was designed to evaluate the antiseptic efficacy of tinidazole stilus against periodontal pathogen. | 2001 | 12539636 |
| bacteremia and sepsis due to prevotella oris from dentoalveolar abscesses. | | 2003 | 12664220 |
| antimicrobial properties of self-etching primer-bonding systems. | self-etching primers are now considered the new generation of dentin bonding systems that modify and incorporate the bacteria-containing smear layer into their bonding mechanism. the antibacterial effects of the self-etching primers clearfil se bond, mac bond, imperva fl bond, one-up bondf and prompt l-pop were evaluated using the bacteria streptococcus mutans atcc25175, peptostreptococcus anaerobius, peptostreptococcus prevotii, peptostreptococcus asaccharolyticus, lactobacillus acidophilus, la ... | 2003 | 12670069 |
| cervical spinal epidural abscess and meningitis due to prevotella oris and peptostreptococcus micros after retropharyngeal surgery. | | 2004 | 15034651 |
| prevotella shahii sp. nov. and prevotella salivae sp. nov., isolated from the human oral cavity. | two bacterial strains, ehs11(t) and epsa11(t), which were isolated from the human oral cavity, were characterized in terms of phenotypic and biochemical characteristics, cellular fatty acid profiles and phylogenetic position based on 16s rrna gene sequence analysis. 16s rrna gene sequence analysis showed that each of the isolates belonged to a novel species of the genus prevotella. strain ehs11(t) was related to prevotella loescheii (about 95 % similarity), whereas strain epsa11(t) was related t ... | 2004 | 15143039 |
| prevotella massiliensis sp. nov. isolated from human blood. | we report a bacterial isolate (marseille isolate) recovered from the blood of a patient hospitalized in an intensive care unit, presenting with severe trauma, fever and mechanical ventilation. colonies appeared at 37 degrees c on blood agar after 72 h incubation. this isolate was a strictly anaerobic, gram-negative rod phenotypically related to other prevotella species described to date: non-motile, catalase-negative, oxidase-positive, non-glucose fermenting, resistant to vancomycin and suscepti ... | 2005 | 16085394 |
| bilophila wadsworthiain brain abscess: case report. | a case of a patient with a 20-year history of chronic otitis media complicated by cholesteatoma and brain abscess is described. a ct scan with contrast material showed three abscess cavities in the right cerebellar hemisphere. a culture from a specimen of the cholesteatoma yielded a significant amount of growth of bilophila wadsworthia, bacteroides fragilis and prevotella oris and a moderate growth of alpha-streptococci and staphylococcus simulans. from the pus of the brain abscess we also isola ... | 1997 | 16887572 |
| identification of bacteria associated with spreading odontogenic infections by 16s rrna gene sequencing. | to determine the bacterial species associated with spreading odontogenic infections (sois). | 2007 | 17141534 |
| prevotella timonensis sp. nov., isolated from a human breast abscess. | gram-negative anaerobic rods were isolated from a human breast abscess. based on genotypic and phenotypic characteristics, the novel strain belonged to the genus prevotella. phylogenetic analysis based on 16s rrna gene sequence comparisons showed that it was closely related to prevotella buccalis (94 % 16s rrna gene sequence similarity), prevotella salivae (90 %) and prevotella oris (89.1 %). the major cellular fatty acid was c(14 : 0) (19.5 %). the new isolate represents a novel species in the ... | 2007 | 17392225 |
| use of multiple-displacement amplification and checkerboard dna-dna hybridization to examine the microbiota of endodontic infections. | multiple-displacement amplification (mda) has been used to uniformly amplify bacterial genomes present in small samples, providing abundant targets for molecular analysis. the purpose of this investigation was to combine mda and checkerboard dna-dna hybridization to examine the microbiota of endodontic infections. sixty-six samples were collected from teeth with endodontic infections. nonamplified and amplified samples were analyzed by checkerboard dna-dna hybridization for levels and proportion ... | 2007 | 17634304 |
| development of a novel pcr assay for detection of prevotella oris in clinical specimens. | prevotella oris is a nonpigmented, gram-negative, anaerobic bacterium that has been associated with several serious oral and systemic infections. prevotella oris has been identified in clinical specimens by bacterial culture and biochemical tests, which are generally unreliable. the aim of this study was to develop a pcr assay for the direct detection of p. oris in clinical specimens. pcr primers specific for p. oris were identified by alignment of bacterial 16s rrna genes from closely related s ... | 2007 | 17937671 |
| prevotella maculosa sp. nov., isolated from the human oral cavity. | three strains of anaerobic gram-negative bacilli isolated from human oral sites were subjected to a comprehensive range of phenotypic and genotypic tests and were found to comprise a homogeneous group. 16s rrna gene sequence analysis revealed the strains to constitute a novel group within the genus prevotella, most closely related to prevotella oris and prevotella salivae. a novel species, prevotella maculosa sp. nov., is proposed to accommodate these strains. prevotella maculosa is saccharolyti ... | 2007 | 18048753 |
| trends in antibiotic resistance in prevotella species from patients of the university hospital of maxillofacial surgery, sofia, bulgaria, in 2003-2009. | head-and-neck infections often involve anaerobes such as prevotella species. aim of the present study was to assess the evolution and the factors associated with resistance in prevotella species to penicillin, clindamycin, metronidazole, tetracycline and β-lactams/β-lactamase inhibitors (bl/blis). in total, 192 prevotella strains, isolated from patients with oral and head-and-neck infections, were evaluated. common isolates were prevotella intermedia and prevotella melaninogenica within the pigm ... | 2010 | 20670687 |
| stimulants of toll-like receptors 2 and 4 are elevated in saliva of periodontitis patients compared with healthy subjects. | because the absorption of stimulants of toll-like receptor (tlr)2 and tlr4 from the gastrointestinal tract into the circulation has been proposed to promote the development of atherosclerosis and insulin resistance, we aimed to quantify the abundance of stimulants of tlr2 and tlr4 in human saliva. | 2011 | 21284689 |
| exploring bacterial diversity of endodontic microbiota by cloning and sequencing 16s rrna. | the characterization of microbial communities infecting the endodontic system in each clinical condition may help on the establishment of a correct prognosis and distinct strategies of treatment. the purpose of this study was to determine the bacterial diversity in primary endodontic infections by 16s ribosomal-rna (rrna) sequence analysis. | 2011 | 21689545 |
| correlation network analysis applied to complex biofilm communities. | the complexity of the human microbiome makes it difficult to reveal organizational principles of the community and even more challenging to generate testable hypotheses. it has been suggested that in the gut microbiome species such as bacteroides thetaiotaomicron are keystone in maintaining the stability and functional adaptability of the microbial community. in this study, we investigate the interspecies associations in a complex microbial biofilm applying systems biology principles. using corr ... | 2011 | 22163302 |
| robust species taxonomy assignment algorithm for 16s rrna ngs reads: application to oral carcinoma samples. | usefulness of next-generation sequencing (ngs) in assessing bacteria associated with oral squamous cell carcinoma (oscc) has been undermined by inability to classify reads to the species level. | 2015 | 26426306 |
| microbial ecosystem analysis in root canal infections refractory to endodontic treatment. | the purpose of this study was to combine multiple displacement amplification and checkerboard dna-dna hybridization to qualitatively and quantitatively evaluate the microbiota present in infections refractory to endodontic treatment. | 2016 | 27377440 |
| molecular methods for diagnosis of odontogenic infections. | historically, the identification of microorganisms has been limited to species that could be cultured in the microbiology laboratory. the purpose of the present study was to apply molecular techniques to identify microorganisms in orofacial odontogenic infections (ois). | 2012 | 22326175 |
| age-dependent changes in porphyromonas gingivalis and prevotella species/phylotypes in healthy gingiva and inflamed/diseased sub-gingival sites. | early colonisation of oral surfaces by periodontal pathogens presents a significant risk factor for subsequent development of destructive disease affecting tissues that support the dentition. the aims of the present study were to establish the age-dependent relationship between sub-gingival profiles of 22 prevotella species/phylotypes in children, adolescents and adults from an isolated aboriginal community and, further, to use this information to identify prevotella species that could serve as ... | 2015 | 25106846 |
| coaggregation between prevotella oris and porphyromonas gingivalis. | the coaggregation of bacteria has been defined as one of the most important processes in the oral infection such as periodontitis. prevotella oris and porphyromonas gingivalis, which are two of the periodontopathogens, are frequently detected in severe forms of periodontal diseases. however, the interaction between p. oris and p. gingivalis is still unknown. in this study, the coaggregation of p. oris with nine oral bacterial species including p. gingivalis was examined. | 2014 | 23245806 |
| the release of glyceraldehyde-3-phosphate dehydrogenase (gapdh) from human erythrocyte membranes lysed by hemolysin of prevotella oris. | we found that a 38-kda protein was released from erythrocyte membranes lysed by hemolysin of prevotella oris, although hypotonic hemolysis did not show such a phenomenon. the 38-kda protein was identified as glyceraldehyde-3-phosphate dehydrogenase (gapdh) by n-terminal amino acid sequencing. this study discusses the relationship between gapdh and hemolysis. | 2012 | 22967794 |
| slowly growing, ulcerating nodule on the posterior ankle . | a 34-year-old haitian man presented with a 1-year history of a gradually en- larging, ulcerating nodule on the right posterior ankle that bled after trauma. the patient denied any history of prior trauma at the site of the lesion and foreign travel.there were no hiv risk factors or personal or family history of skin cancer.the patient was otherwise healthy with no additional complaints. physical examination revealed a 1.4 cm firm, hyperpigmented nodule with a yellow crateriform center ... | 2011 | 25881109 |