Publications

TitleAbstractYear
Filter
PMID
Filter
bacterial interference by oropharynegeal and clinical isolates of anaerobic bacteria.anaerobic isolates were tested for bacterial inhibitory activity. of 144 isolates, 102 were from oropharynegeal washings, and 42 were from clinical specimens. thirteen facultative bacterial species (seven members of the enterobacteriaceae and six species of gram-positive cocci) were used as indicators of inhibition. eleven anaerobic species were isolated from oral secretions. all isolates of bacteroides melaninogenicus, the most commonly recovered species, consistently inhibited several species ...197610336
beta-lactamase activity in strains of bacteroides melaninogenicus and bacteroides oralis.beta-lactamase from strains of bacteroides melaninogenicus and bacteroides oralis hydrolyzed penicillin more rapidly than ampicillin or carbenicillin. cephalothin and a chromogenic cephalosporin (87/312) were also hydrolyzed by the enzyme. activity was found only in beta-lactam-resistant strains, but there was considerable variation in activity among strains having the same minimal inhibitory concentrations of antibiotic. beta-lactamase activity was cell bound and appeared to be tightly associat ...197713705
the syndrome of inappropriate secretion of antidiuretic hormone associated with anaerobic thoracic empyema.the syndrome of inappropriate secretion of antidiuretic hormone has been associated with many pulmonary diseases, including tuberculosis and bacterial and viral pneumonia: however, it has not been reported with anaerobic infections or empyema in the absence of pneumonia. we report a patient with empyema due to bacteroides melaninogenicus, bacteroides oralis, and peptostreptococcus who developed the syndrome. eight hours before the start of therapy, his serum sodium concentration was 127 meq per ...197715491
chemical composition, serological reactivity and endotoxicity of lipopolysaccharides extracted in different ways from bacteroides fragilis, bacteroides melaninogenicus and bacteroides oralis.lipopolysaccharides (lps) extracted from strains of bacteroides fragilis, bacteroides melaninogenicus and bacteroides oralis with phenol-water, trichloroacetic acid, edta or liquid phenol-chloroform-petroleum ether (pcp) and isolated by ultracentrifugation, varied considerably in their quantitative chemical composition. negligible yields of lps were obtained by pcp-extraction. all preparations were more or less serologically active. all methods (except pcp) extracted the same o-antigenic determi ...197719922
human complement activation by lipopolysaccharides from bacteroides oralis, fusobacterium nucleatum, and veillonella parvula.the properties of different lipopolysaccharide (lps) preparations to induce c3 conversion in human serum was studied by means of crossed immunoelectrophoresis. c3 conversion by the alternative pathway was evaluated after calcium depletion, and lipid a-dependent activation was measured by means of inhibition with polymyxin b sulfate. lps from bacteroides oralis converted co mainly via the alternative pathway, whereas lps from fusobacterium nucleatum and veillonella parvula const pronounced lipid ...1979121108
the fetid foot: lower-extremity infections in patients with diabetes mellitus.infections of the lower extremities in patients with diabetes mellitus have been attributed to staphylococcus aureus and other facultatively anaerobic bacteria. however, a review of 30 consecutive diabetics who required surgery for lower-extremity infections revealed that 17 had mixed infections due to both obligate and facultative anaerobes; only six had infections due to s. aureus. mixed infections often had characteristics of anaerobic suppuration and responded poorly to therapy. patients wit ...1979318225
a special report: four-year study of a boy with combined immune deficiency maintained in strict reverse isolation from birth.a 4-year study of a boy with combined immune deficiency is presented, and the impact of this disease on various aspects of his growth and development is examined. there is no evidence of immune deficiency in either parent or in the genetic background on the maternal side. three children of a brother of the mother's father may have had immune deficiencies but two have grown to be teenagers with no problems. another died. at autopsy, however, lymph nodes appeared normal. the deceased older brother ...1977401538
endotoxic activities of lipopolysaccharides of microorganisms isolated from an infected root canal in macaca cynomolgus.lipopolysaccharides (lps) prepared from a strain of bacteroides oralis, a strain of fusobacterium necrophorum, and a strain of f. nucleatum, all isolated from an infected root canal in monkey (macaca cynomolgus), were examined for endotoxic activities using primary skin reactions in rabbits and induction of leukocyte chemotaxis in rats. lps of b. oralis showed considerably lower ability to cause skin inflammation than lps of the fusobacteria. however, the leukotactic effect of the lps preparatio ...1977405737
immunoelectron microscopic localization of lipopolysaccharides in the cell wall of bacteroides oralis and fusobacterium nucleatum.lipopolysaccharides (lps) have been extracted and purified from two anaerobic gram-negative bacteria: bacteroides oralis and tfusobacterium nucleatum. chemical analysis of the preparations showed a great proportion of neutral sugars, mainly glucose, in lps of b. oralis. in rabbits, lps of b. oralis induced both immunoglobulin m and g antibodies in contrast to lps of f. nucleatum, to which only immunoglobulin m antibodies were produced. an immunohistochemical method with horseradish peroxidase-la ...1978624589
[in vitro susceptibility of bacteroidaceae to cefoxitin and cephalothin (author's transl)].the activity of the new cephamycin c derivatives cefoxitin against 102 clinical isolates of bacteroidaceae (95 bacteroides strains and 7 fusobacterium-sphaerophorus cultures) was determined by tube dilution and standardized agar diffusion tests and compared to that of cephalothin. cefoxitin was more active than cephalothin against 56 bacteroides fragilis strains, 19 bacteroides thetaiota-omicron strains and two bacteroides splanchnicus strains. the difference seemed less pronounced with five bac ...1977856727
dextran degrading activity of oral microbial flora.dextran degrading activity of the human oral microflora was detected by culturing in tyd broth (tryptose 1.0%, yeast extract 0.3%, dextran t-150 (pharmacia, mw 150,000) 0.15%). all of the plaue and saliva samples collected from 10 subjects showed a dextran degrading activity, both cultured aerobically and anaerobically, while the anaerobic culture was more active than the aerobic. furthermore, some individual differences were observed in their activity. crude enzyme(s) was extracted from the sup ...19761065508
the endodontic microflora revisited.the microbial flora of 35 dental root canals were examined, taking care to maintain the viability of obligate anaerobes which accounted for 45% of total isolations, while streptococcal species accounted for 24% of the total species isolated. individual root canals yielded a maximum of eight bacterial species. a total of 40 different species was isolated of which the most prevalent were the facultative anaerobe streptococcus sanguis and the obligate anaerobe, peptostreptococcus micros (both in 23 ...19921479923
diagnosis and management of anaerobic infections of the head and neck.anaerobic bacteria are important pathogens in head and neck infections such as chronic otitis media, chronic sinusitis, chronic mastoiditis, head and neck abscesses, cervical adenitis, parotitis, and postoperative infection. bacteroides sp (bacteroides melaninogenicus group, bacteroides oralis, and bacteroides fragilis group), peptostreptococcus sp, and fusobacterium sp predominate. the observed recent increase in the number of beta-lactamase-producing strains of bacteroides sp isolated in head ...19921728904
[bacteriological examination of infections in the field of oral surgery].etiology of bacterial infections in the field of oral surgery was studied. a total of 270 samples collected from patients with encapsulated abscess in their oral cavities was examined and bacteria were isolated from the 244 samples (90.4%). the following results were found; 1) organisms more than one from one sample were frequently isolated from cases with parodontitis, pericoronitis and gnathitis. isolation of anaerobic bacteria was common (54.2%). 2) streptococcus milleri and streptococcus san ...19911791332
comparative in vitro activity of cefpodoxime against anaerobes other than bacteroides fragilis.to assess the in vitro activity of cefpodoxime against anaerobic respiratory tract and oropharyngeal pathogens 77 strains belonging to 18 gram-negative and 7 gram-positive species were studied by means of agar dilution tests. for comparison cefuroxime, amoxicillin, amoxicillin + clavulanic acid and clindamycin were also tested. cefpodoxime was found to be active at concentrations of less than or equal to 0.125 mg/l against prevotella oralis, prevotella buccalis, prevotella bivia, porphyromonas a ...19911800380
[bacteriology of abdominal pus in 43 cases of acute appendicitis and appendiceal abscess at the ivrea-castellamonte hospital, isolation of aerobic and anaerobic bacteria and drug sensitivity].a retrospective study on the microbiology of abdominal pus from acute appendicitis or peritonitis was carried out by the authors. a total of 45 specimens were examined, 38 of them (84.4%) where found to be positive, of which 29 (76.3%) were polymicrobial and 9 (23.7%) were monomicrobial. the most represented species were escherichia coli (28.4%), bacteroides fragilis (7.8%), streptococcus milleri (7.8%), bacteroides oralis (3.9%) and pseudomonas aeruginosa (3.9%). the polymicrobial associations ...19911844922
the microflora of the lower respiratory tract of the horse: an autopsy study.a series of 31 thoroughbreds and 18 non-thoroughbreds was examined post mortem to assess what bacteria might be present in the lower respiratory tract, and to assess their significance by correlating the bacteriological findings with histological changes in the lung. the distal trachea and seven representative lung sites were swabbed in each case. sixty-six per cent of the sites were sterile with remaining sites producing scanty growths of mainly coagulase negative staphylococcus spp., e. coli, ...19911878768
multivariate analyses of cellular fatty acids in bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp.the genera bacteroides, wolinella, and campylobacter contain several similar species that require taxonomic revision. fatty acid profiles of whole bacterial cells have proven useful for taxonomy. in this study, cellular fatty acids from bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp. were identified and quantitated by gas chromatography and gas chromatography-mass spectrometry, and the data were subjected to principal component analyses. bacteroides fragilis, the type s ...19911993755
eikenella corrodens infection of the oral cavity as a cause of bacterial endocarditis.the authors describe the case of a 24-year-old woman with valve disease. after a bout of respiratory tract infection, she was diagnosed to have bacterial endocarditis associated with mitral valve disease. the causative agents isolated included eikenella corrodens, streptococcus intermedius, bacteroides oralis and bacteroides bivius. at the same time, the patient was found to have developed iga immunodeficiency. a complication accompanying the cardiac disease was spontaneous pneumothorax. since a ...19902190761
comparative in vitro activity of cefpirome and cefepime, two new cephalosporins.in in vitro tests the broad-spectrum cephalosporins cefpirome and cefepime were highly active against enterobacteriaceae, although often less so against strains resistant to amoxicillin-clavulanate and ticarcillin-clavulanate, and against most strains of acinetobacter spp. and aeromonas hydrophila. they were also active against pseudomonas aeruginosa, although strains with non-plasmid mediated beta-lactam resistance were sometimes less sensitive. other pseudomonas spp. varied in their sensitivit ...19902226498
quantitative bacteriology of acute dento-alveolar abscesses.a qualitative and quantitative bacteriological study was performed on pus specimens obtained by needle aspiration of 50 acute dento-alveolar abscesses. most samples contained a mixture of species (average 3.3); 20 (40%) of the abscesses contained anaerobes alone, 3 (6%) contained facultative anaerobes only and the remaining 27 (54%) contained mixtures of both types of bacteria, with anaerobes predominating. in total, 166 bacterial strains were isolated, 75% of which were strictly anaerobic; the ...19862869147
use of the rapid-ana system to screen for enzyme activities that differ among species of bile-inhibited bacteroides.the rapid-ana system (innovative diagnostics systems, inc., atlanta, ga.) was used to test 102 strains of 14 species of phenotypically similar bile-inhibited bacteroides from humans. bacteroides oris, bacteroides veroralis, bacteroides buccalis, bacteroides melaninogenicus, bacteroides loescheii, and bacteroides denticola had very similar enzyme activity profiles. clear differentiation of these six species by the rapid-ana system was not possible, but tests for arginine aminopeptidase and beta-g ...19862871042
an electron microscope survey of the surface structures and hydrophobicity of oral and non-oral species of the bacterial genus bacteroides.seventeen strains of bacteroides representing 10 species were examined by negative staining; the majority were from the mouth but a few non-oral strains were included. seven species had peritrichously-arranged, non-flagellar appendages which could be divided by morphology and ultrastructure into two subgroups, fibrils and fimbriae. bacteroides asaccharolyticus strains b536 and b537 and bacteroides gingivalis strains w50, w83, wph15 and wph35 had fimbriae with mean width of 4.4 nm and 0.5 to 6.0 ...19862875705
anaerobic infections in childhood.bacteroides melaninogenicus and bacteroides oralis are predominant anaerobes in orofacial infections and aspiration pneumonia. fusobacterium species are common pathogens in aspiration pneumonia, brain abscesses and orofacial infections. clostridium perfringens can cause bacteremia and wound infections. clostridium botulinum can produce a paralytic toxin that causes a paralytic syndrome in infants. clostridium difficile can cause diarrhea or antibiotic-associated colitis.19862876620
ischaemic mouse thigh model for evaluation of pathogenicity of non-clostridial anaerobes.a mouse thigh model has been devised in which the growing culture of non-clostridial anaerobe in the ischaemic tissue produces inflammatory swelling and death. the swelling of the right thigh served as an index of pathogenicity of the test strain in comparison to the negatively reacting left thigh which received injection of the control strain of bifidobacterium infantis. actinomyces naeslundii exceptionally caused death in all animals within 24 h. mortality and thigh swelling were pronounced (g ...19892914729
the role of beta-lactamase-producing bacteria in respiratory tract infections.staphylococcus aureus, haemophilus influenzae, bacteroides sp. (bacteroides melaninogenicus, bacteroides oralis, and bacteroides fragilis), peptostreptococci and fusobacterium sp. are important pathogens in respiratory tract infections (rti). these organisms are often recovered mixed with other aerobic, facultative and anaerobic bacteria. a recent increase in numbers of bet-lactamase producing strains of anaerobic gram-negative bacteria in rti has been associated with increased failure rates of ...19883074471
recovery of beta-lactamase producing bacteria in pediatric infections.the presence of beta-lactamase producing bacteria (beta lpb) was investigated in specimens obtained from 1469 children who presented with infections of the skin and soft tissue (648), upper respiratory tract (514), pulmonary sites (137), surgical sites (113), and other (57). of 4989 bacterial isolates recovered, 910 (18%) were beta lpb, 492 (54%) aerobes, and 418 (46%) anaerobes. the beta lpb were recovered in 751 (51%) of the children. the most frequently recovered beta lpb was staphylococcus a ...19873121158
emergence and persistence of beta-lactamase-producing bacteria in the oropharynx following penicillin treatment.the emergence and persistence of aerobic and anaerobic beta-lactamase-producing bacteria (blpb) were investigated in 26 children treated with penicillin for otitis media or pharyngitis and in 28 nontreated control children. beta-lactamase-producers were isolated in three (12%) of the treated children before therapy, in 12 (46%) seven to ten days after completion of therapy, in nine (35%) 40 to 45 days after therapy, and in seven (27%) 85 to 90 days after therapy. these organisms were present in ...19883130087
in vitro activity of flomoxef compared to moxalactam, cefoxitin, cefotaxime, and clindamycin against anaerobes.to assess the in vitro activity of flomoxef (6315-s), moxalactam, cefoxitin, cefotaxime, and clindamycin against anaerobes 197 clinical isolates (27 bacteroides fragilis, 42 b. thetaiotaomicron, 10 b. vulgatus, 7 b. ovatus, 6 b. uniformis, 6 b. distasonis, 7 bacteroides melaninogenicus group, 11 bacteroides oralis group, 21 clostridium difficile, 7 c. perfringens, 3 c. sporogenes, 3 clostridium spp., 33 propionibacterium acnes, 14 peptococcaceae) were studied by means of agar dilution tests. the ...19883214438
the presence of cytochromes in oral anaerobic bacteria, especially in bacteroides oralis. 19883255150
purification and characterization of intracellular dextranase of bacteroides oralis ig4a.multiple forms of dextranase were detected in both intra- and extracellular fractions of bacteroides oralis ig4a. the molecular weights of these enzymes varied from 52,000 to 260,000 by sodium dodecyl sulfate-polyacrylamide-blue dextran gel electrophoresis. the intracellular dextranases were fractionated by chromatography and gel filtration steps, and the dextranases iv and v were obtained. the former was only partially pure. the molecular weights of the dextranases iv and v were estimated to be ...19883270938
anaerobic wound infection: bacteroides mediastinitis after cardiovascular surgery.a case of postoperative anaerobic mediastinitis after coronary artery bypass grafting is reviewed. one of the causative organisms, bacteroides oralis, has never previously been described as a pathogen causing mediastinitis after median sternotomy incision. there was associated bacteroides fragilis bacteremia. only three cases of bacteroides species mediastinitis after open heart surgery have been reported. this anaerobic bacterium remains a rare pathogen in median sternotomy infections despite t ...19883292461
[frequency of isolation and antibiotic sensitivity of bacteroides related to bacteroides bivius and bacteroides oralis].274 strains of the bacteroides oralis-bivius group are studied: 112 are identified to bacteroides bivius or b. disiens, 73 to bacteroides oralis and 49 to bacteroides oris or bacteroides buccae. these strains are isolated from clinical sample: gynecologic suppurations or respiratory tract infections. the susceptibility of 63 strains to 7 antibiotics were determined. tested antibiotics were: cefalotin, cefoxitine, cefotaxime, mezlocillin, clindamycin, metronidazole and colistine. cefalotine has a ...19873302864
direct and indirect pathogenicity of anaerobic bacteria in respiratory tract infections in children.bacteroides sp (bacteroides melaninogenicus, bacteroides oralis, and bacteroides fragilis), peptostreptococci, and fusobacteria are important pathogens in respiratory tract infections (rti). these organisms are often recovered mixed with other aerobic, facultative, and anaerobic bacteria. evidence supporting their virulence is provided by studies showing their synergistic potentials in mixed infections and their increased virulence when encapsulated. a recent increase in numbers of beta-lactamas ...19873318301
[sensitivity of anaerobes to 8 antibiotics].we studied the susceptibility of 282 obligate anaerobes to 8 antibiotics. the strains were isolated from clinical specimens taken during 1983 and 1984. minimal inhibitory concentrations (mics) were determined using a microplate method for 245 strains and agar dilution for 33 bacteroides asaccharolyticus strains. tested strains were as follows: 85 bacteroides fragilis, 71 clostridium, 19 fusobacterium nucleatum, 35 peptostreptococcus, 39 bacteroides oralis and bacteroides bivius and 33 bacteroide ...19863534760
enzymatic and antigenic characterization of immunoglobulin a1 proteases from bacteroides and capnocytophaga spp.bacteroides and capnocytophaga species have been implicated as periodontal pathogens. some of these species possess immunoglobulin a1 (iga1) proteases that are capable of cleaving the human iga1 molecule in the hinge region, leaving intact fc alpha and fab alpha fragments. the purpose of this study was to characterize this activity. in addition to iga1 protease activity in already known species, iga1 protease activity was a feature of bacteroides buccalis, bacteroides oralis, bacteroides veroral ...19873546133
role of anaerobic beta-lactamase-producing bacteria in upper respiratory tract infections.bacteroides sp. (bacteroides melaninogenicus, bacteroides oralis and bacteroides fragilis), peptostreptococci and fusobacterium sp. are important pathogens in upper respiratory tract infections. a recent increase in numbers of beta-lactamase-producing strains of anaerobic gram-negative bacteria in upper respiratory tract infections has been associated with increased failure rates of penicillins in eradication of these infections. these infections include chronic otitis media, chronic sinusitis a ...19873554127
anaerobic osteomyelitis in children.twenty-six pediatric patients with osteomyelitis caused by anaerobic bacteria are presented. the etiologic factors were chronic mastoiditis (7 patients), decubitus ulcers (5), chronic sinusitis (4), periodontal abscesses (3), bites (3), paronychia (2), trauma (1) and scalp infection after fetal monitoring (1). seventy-four organisms (2.8 isolates/specimen), including 63 anaerobes (2.4/specimen), and 11 facultative and aerobic bacteria (0.4/specimen) were recovered. the predominant organisms were ...19863763419
microbiology of retropharyngeal abscesses in children.aspiration of retropharyngeal abscesses was performed in 14 children. cultures were taken from aspirates for aerobic and anaerobic bacteria, and all yielded bacterial growth. anaerobes were isolated in all patients; they were the only organisms isolated in two patients (14%) and were mixed with aerobes in 12 patients (86%). there were 78 anaerobic isolates (5.6 per specimen). the predominant anaerobes were bacteroides species, peptostreptococcus species, and fusobacterium species. there were 26 ...19873812388
prevalence of beta-lactamase-producing bacteria in chronic suppurative otitis media.aspiration of the exudate through open perforation was performed in 48 children with chronic suppurative otitis media. eighty-two aerobic and 93 anaerobic isolates were recovered. aerobic bacteria only were involved in 22 patients (46%), and anaerobic organisms only were involved in five patients (10%). mixed aerobic and anaerobic isolates were recovered from 21 patients (44%). the most common bacteria isolated were bacteroides melaninogenicus group (40% of patients), pseudomonas aeruginosa (29% ...19853872063
dental pulp response to bacterial cell wall material.lipopolysaccharides (lps) from bacteroides oralis and veillonella parvula and cell wall material from lactobacillus casei were studied for their capacity to induce leukocyte migration in the dental pulp and in an implanted wound chamber. three adult monkeys were challenged using lyophilized material sealed into buccal class v cavities prepared in dentin. pulp tissue responses were observed histologically eight and 72 hours after initiation of the experiment. subjacent to cut dentinal tubules, ba ...19853926844
subcellular localization of d-glucanases in bacteroides oralis ig4a.three d-glucan-hydrolysing enzymes from bacteroides oralis ig4a have been isolated. two of them are dextranases which hydrolyse (1----6) but not (1----3) linked alpha-d-glucans; one (ec 3.2.1.11, 1,6-alpha-d-glucan 6-glucanohydrolase) is localized in the periplasm, and the other, which is an exo-enzyme (ec 3.2.1.70, 1,6-alpha-d-glucan glucohydrolase), in the cytoplasm. the third is a mutanase (ec 3.2.1.59, 1,3-(1,3;1,4)-alpha-d-glucan 3-glucanohydrolase) that hydrolyses (1----3) but not (1----6) ...19854020343
isolation, characterization and ecological distribution of recently recognized propionic acid-negative bacteroides species from human sources.140 strains of anaerobic, gram-negative, propionate-negative, non-pigmented rods (bacteroides oralis group) were isolated from normal flora and from clinical specimens. they were characterized by physiological tests, gas chromatography of the acid end products, and determination of the g + c content of their dna. in addition, their distribution in the various biotopes of the human body (oropharyngeal tract, genitourinary tract, and gut) and in clinical specimens was investigated. the strains cou ...19854050192
anaerobic bacteria in 21 horses with pleuropneumonia.anaerobic bacteria are important and overlooked bacterial pathogens of the lower respiratory tract in horses. twenty-one of 46 horses with pleuropneumonia had anaerobic bacteria isolated from pleural fluid or from tracheobronchial aspirate. bacteroides oralis and b melaninogenicus were the anaerobes most frequently isolated. survival was significantly less for horses from which anaerobes were isolated than for horses from which anaerobes were not isolated. putrid odor was associated with the ple ...19854055490
effect of bacterial contamination on cecal size and cecal contents of gnotobiotic rodents.in the present investigation the effect of various bacterial contaminations of gnotobiotic mice and rats on cecal size is presented. of the species tested, bacteroides oralis and fusobacterium nucleatum did not establish in germ-free mice. streptococcus mutans, clostridium difficile, a neisseria strain and two recent cecal isolates established, but failed to exert an effect upon the cecum of mice. a group k streptococcus and b. fragilis increased the cecal size apparently by increasing the level ...19695808078
beta-lactamase producing bacteria in the human oral cavity.twenty-six individuals who had no history of long-term antibiotic therapy were examined for the prevalence of beta-lactamase producing bacteria in the oral cavity. samples from a total of 159 normal and diseased periodontal sites, 44 cheek mucosae, 22 tongue dorsa and 22 salivas were studied. penicillin resistant organisms were recovered from trypticase soy blood agar plates containing 1.3 microgram/ml or 2 microgram/ml benzylpenicillin. beta-lactamase formation by these isolates was determined ...19826121020
complement-dependent histamine release from rat peritoneal mast cells, induced by lipopolysaccharides from bacteroides oralis, fusobacterium nucleatum and veillonella parvula.the capacity of phenol/water extracted lipopolysaccharides (lps) from bacteroides, fusobacterium and veillonella to induce histamine release from rat peritoneal mast cells has been evaluated by means of a bioassay using atropinized guinea pig ileum and a fluorescence assay. histamine release was found to be completely complement dependent. it was also found that lps of veillonella have the greatest capacity to induce histamine release and bacteroides have the weakest.19816168752
role of anaerobic bacteria in bite-wound infections.the etiologic agents usually involved in wound infections due to human or animal bites are the aerobic skin flora of the victim, e.g., staphylococcus aureus, and/or the aerobic oral flora of the biter, e.g., pasteurella multocida. while anaerobic bacteria are predominant in the normal oral flora of humans and animals, their importance in the pathogenesis of bite-wound infections has not been stressed. most investigators in this field have either not cultured these wounds for anaerobic bacteria o ...19846372026
anaerobic infections in childhood.anaerobic bacteria are part of the normal flora of mucous membranes and outnumber aerobic bacteria in the oral cavity and gastrointestinal tract. anaerobes can be isolated from pediatric patients with various infections when appropriate techniques for transportation and cultivation of samples are employed. frequently anaerobes are isolated in combination with other facultative or aerobic bacteria. the genera or groups of anaerobes most frequently isolated from pyogenic infections in children are ...19846372028
the role of beta-lactamase-producing bacteria in the persistence of streptococcal tonsillar infection.the failure of penicillin to eradicate group a beta-hemolytic streptococci (gabhs) is a growing problem. this review summarizes current data concerning the role of aerobic and anaerobic beta-lactamase-producing bacteria in the persistence of tonsillar infection caused by gabhs. clinical studies have demonstrated the recovery of beta-lactamase-producing bacteria and detectable levels of the enzyme beta-lactamase in cultures of core tonsillar specimens in greater than 80% of the patients. the pred ...19846390637
the presence of beta-lactamase-producing bacteria as a guideline in the management of children with recurrent tonsillitis.thirty-eight children who had recurrent tonsillitis and who were chronic carriers of group a beta-hemolytic streptococci (gabhs) were treated with oral clindamycin. surface tonsillar cultures, obtained prior to therapy and two weeks after the termination of therapy, were processed for aerobic and anaerobic microorganisms. mixed aerobic and anaerobic flora were obtained from all cultures. prior to therapy, the average yield was 9.3 isolates (5.2 aerobes and 4.1 anaerobes) per specimen; after the ...19846400716
[in vitro activity of the oxa-beta-lactam ly 127935 against bacteroides fragilis and other anaerobic gram-negative rods (author's transl)].the susceptibility of 115 bacteroidaceae strains to ly 127935 was determined by broth dilution, agar dilution and agar diffusion tests. simultaneously, the strains were tested for degradation of the oxa-beta-lactam. only five strains (3 bacteroides fragilis and 3 bacteroides oralis/bacteroides bivius) partially degraded ly 127935. no major discrepancies between the results of the agar dilution and broth dilution tests were observed. according to the results obtained by the agar dilution method, ...19806447673
beta-lactamase-producing bacteria recovered after clinical failures with various penicillin therapy.the presence of beta-lactamase-producing bacteria in clinical specimens was investigated in 185 children with orofacial or respiratory tract infections. all of these patients failed to respond to antimicrobial therapy, including penicillins, that was administered to 148 (80%) of them. beta-lactamase-producing aerobic and anaerobic bacteria were detected in 75 (40.5%) of the 185 children. the beta-lactamase-producing strains included all 11 strains of the bacteroides fragilis group, 30 (45.4%) of ...19846608346
infectivity of organisms recovered from polymicrobial abscesses.the ability to cause subcutaneous abscesses in mice was used to identify the pathogens among the bacteria recovered from 13 clinical abscesses. a total of 35 isolates (30 anaerobes and 5 aerobes), 16 of which were encapsulated, were recovered from these abscesses. encapsulated organisms included eight bacteroides spp. (three bacteroides asaccharolyticus and one strain each of bacteroides oralis, bacteroides intermedius, bacteroides biacutus, bacteroides vulgatus, and bacteroides ruminicola subsp ...19836642674
apical periodontitis induced by selected bacterial strains in root canals of immunized and nonimmunized monkeys.experimental infections in root canals with streptococcus faecalis ss. liquefaciens (strep-mc4). actinomyces bovis (act-mc6) and bacteroides oralis (bact-mc3) were studied in four immunized and five nonimmunized monkeys. the immunization was performed using antigens of the homologous strains. the antibody level was checked by gel diffusion. in 56 root canals of the immunized animals and 60 root canals of the nonimmunized animals, viable cells of the three bacterial strains were enclosed in equal ...19826810447
lipid a in anaerobic bacteria.the ability of lipid a preparations from strains of bacteroides, fusobacterium, and veillonella to inhibit the lipid a-anti-lipid a reaction in an enzyme-linked immunosorbent assay was tested. anti-lipid a serum was prepared with lipid a from salmonella minnesota r595, and lipid a from escherichia coli eh100 was used as control antigen. preparations from three of four different species of bacteroides were unable to inhibit the anti-lipid a activity, whereas lipid a preparations from fusobacteriu ...19836822431
immune response in rats against lipopolysaccharides of fusobacterium nucleatum and bacteroides oralis administered in the root canal.lipopolysaccharides (lps) prepared from one strain of fusobacterium nucleatum and one strain of bacteroides oralis were examined for immunological responses in rats. the lps were applied to the pulp chamber of the two mandibular incisors. using the plaque forming cell (pfc) method both antigens showed a rapid igm response in the spleen, and a slower one in the submandibular lymph nodes. in comparison with the igm response, the igg response was somewhat slower and weaker for lps of bacteroides, w ...19806929559
insoluble glucan hydrolyzing enzymes from oral bacteroides.crude enzymes (en(c)) which hydrolyze insoluble glucan produced by streptococcus mutans fa-1 were extracted from bacteroides oralis obtained from human dental plaque. extracellular insoluble glucan of s. mutans (isg) and the one which was partially modified by smith degradation (m-isg) were used as substrates. commercial dextran (m.w. 2,000,000) was used as control. composition of the types of glucosidic linkages of the glucans was determined by methylation analysis. the ratio of the alpha-(1--6 ...19806930330
bacteriologic evaluation of the efficacy of mechanical root canal instrumentation in endodontic therapy.the presence of bacteria in 17 single-rooted teeth, with periapical lesions, was studied throughout a whole period of treatment. the root canals were irrigated with physiologic saline solution during instrumentation. no antibacterial solutions or dressings were used. bacteria were found in all initial specimens from the teeth (median number of bacterial cells 4 x 10(5), range 10(2) - 10(7)) and the number of strains in the specimens ranged from 1 to 10.88% of the strains were anaerobic. the most ...19816947391
circulating antibodies after experimental chronic infection in the root canal of teeth in monkeys.root canals of 49 teeth with necrotic pulp tissue in five monkeys were infected with streptococcus faecalis ss liquefaciens, actinomyces bovis, fusobacterium nucleatum, peptostreptococcus anaerobius, and bacteroides oralis in various combinations. after 6 months the root canals and the periapical tissues were subjected to radiographic, bacteriologic, and histologic examinations. signs of periapical inflammation were radiographically registered in 41 teeth, most frequently in teeth inoculated wit ...19826960463
simple disk technique for carbohydrate fermentation and esculin hydrolysis testing of anaerobic gram-negative bacilli.carbohydrate fermentation by anaerobic gram-negative bacilli was easily detected using taxo-disks placed on supplemented columbia agar. after incubation, two drops of a 0.2% solution of bromothymol blue were added to each disk: yellow coloring was indicative of a positive reaction. in the esculin hydrolysis test paper disks impregnated with 2 mg of esculin were placed on the same medium. the results were read after addition of two drops of a 10% solution of ferric chloride. when testing slow-gro ...19807005098
the treatment of the carrier state of group a beta-hemolytic streptococci with clindamycin.20 children who were chronic carriers of group a beta-hemolytic streptococci (gabhs) were treated with oral clindamycin. surface tonsillar cultures were obtained prior to therapy and 2 weeks after termination of therapy. they were processed for aerobic and anaerobic microorganisms. mixed aerobic and anaerobic flora were obtained from all cultures. prior to therapy, the average yield was 9 isolates (5 aerobes and 4.1 anaerobes) per specimen; after completion of therapy, the average yield was 5.7 ...19817021079
influence of combinations of oral bacteria on periapical tissues of monkeys.the present study deals with the ability of 11 bacterial strains, in various combinations, to induce periapical reactions. the indigenous oral bacteria were originally isolated from an experimental apical periodontitis in monkey. eight of the strains were complete collection isolated from one root canal. these strains were inoculated together, in equal proportions, into 12 root canals. in addition, 63 canals were inoculated with other combination or separate strains. at the end of the experiment ...19827051261
endocarditis and brain abscess due to bacteroides oralis. 19827086201
isolation and properties of dextranases from bacteroides oralis ig4a.extracellular dextranases were extracted from a dextran-degrading microorganism, bacteroides oralis ig4a, which had been isolated from human dental plaque, and purified. crude enzyme preparations obtained from a broth culture supernatant by salting out with ammonium sulfate were subjected to column chromatography on deae-cellulose and subsequent bio-gel p-100, followed by isoelectric focusing. two kinds of enzyme preparations, enzymes i and ii, with the ability to degrade soluble dextran were ob ...19827121315
neuraminidases of bacteroidaceae die neuraminidasen von bacteroidaceen.neuraminidases can be detected in members of the anaerobic gram-negative non-sporing rods (bacteroidaceae), especially in the genus bacteroides. b. fragilis, the most virulent species, has the highest neuraminidase activity, while the other intestinal species exhibit markedly lower activities or the enzyme is completely absent. members of the bacteroides oralis group, so far investigated, degrade only substrates of lower molecular weight.19817223133
[the role of anaerobic bacteria in the post-partum period: clinical significance and treatment (author's transl)].anaerobic bacteria in the lochia are frequently isolated healthy post-partum patients and from those with post-partum infections. the true pathogenic significance of these anaerobic bacteria is uncertain. investigations appeared to show that the incidence of anaerobic bacteria is by and large the same as in the vaginal and intestinal flora. in this investigation we showed: 1) anaerobic bacteria are rare in the vaginal flora of pregnant patients in the third trimester, 2) a definite difference ex ...19807409423
occurrence of bacteroidaceae in vaginal secretions of healthy pregnant women and patients with colpitis.the frequency of gram-negative non-sporing anaerobes with possible clinical significance was studied in healthy pregnant women and in patients with non-candida vaginitis. only 4% of the healthy pregnant women studied yielded anaerobes of the family bacteroidaceae. however, the incidence of bacteroidaceae in 58 patients with colpitis was 53.5%. the predominant anaerobic species was bacteroides oralis.19807450856
diagnosis of pure and mixed anaerobic infections in obstetrics and gynecology.less than one third of women patients with post-partum endometritis, abscess formation and similar pathologic conditions were found to have pure anaerobic infection. the majority of the infections were caused by facultative organisms and anaerobes. bacteroides oralis, baceroides fragilis and peptococcaceae were the predominant isolates. treatment with an anti-microbial agent active against most anerobes led to clinical cure even in cases of mixed infections. this may possibly indicate that anaer ...19807450867
meningioma associated with intratumoral abscess formation--case report.a rare case of meningioma associated with intratumoral abscess formation occurred in a 64-year-old female presenting with septic meningitis and a right frontal mass lesion after a gynecological operation under spinal anesthesia. the mass lesion was totally removed and revealed as an incidental meningioma with an intratumoral abscess. hematogenous infection of bacteroides oralis was thought to be the cause of the intratumoral abscess formation.19947526233
degradation of immunoglobulin g by periodontal bacteria.several subgingival microorganisms were tested for their ability to utilize human immunoglobulin g (igg) as a substrate for growth. this was done using a protein-free chemically defined medium, supplemented with igg. stimulation of growth was observed for capnocytophaga ochracea, porphyromonas asaccharolytica, porphyromonas endodontalis, porphyromonas gingivalis, prevotella intermedia, prevotella oralis, lactobacillus catenaforme and streptococcus intermedius. immunoelectrophoresis, sodium dodec ...19947870469
microflora in postirradiated cancer cervix.cases of cancer cervix receiving radiation therapy revealed more anaerobes (50%) in comparison to normal controls (44.6%). the aerobic organisms were suppressed in the former cases (63.6%) in contrast to the latter group (90.7%). of the anaerobes, the gram-negative bacilli were more notable (18.1%) in cancer cervix than the normal controls (4.6%). the anaerobes like peptococcus prevotii (18.1%) and bacteroides oralis (9%) and the aerobes, staph aureus (13.6%) and klebsiella (13.6%) were prevalen ...19947963600
cytotoxicity versus antibacterial activity of some antiseptics in vitro.through the development of new techniques for bacterial growth and isolation, obligate anaerobes have been shown to be more prevalent than previously thought in the pathogenesis of periapical and pulpal diseases. a follow-up study was conducted to examine the germicidal effect of 0.05% naocl, 10 mg/ml metronidazole and supernatant of ca (oh)2 (0.025 ml) on four anaerobic microorganisms commonly found in root canals, and to compare the toxicity of these substances on cell cultures. in vitro testi ...19938100851
sternoclavicular joint infection due to bacteroides oralis.a 60-year-old man in good health with no history of intravenous drug abuse was hospitalized for sternoclavicular joint pain and swelling. his complicated course required extensive debridement of the sternoclavicular joint. an insidious course, negative blood culture and requirement for surgery is common in infections of this joint.19938230037
[clinical efficacy of cefmetazole in intraabdominal infection].a number of different organisms can be isolated from intraabdominal infection. the most common are aerobic gram-negative bacilli. anaerobes are not unusual. from june 1989 to january 1990, cefmetazole was administered to 23 patients with intraabdominal infection at veterans general hospital-taipei. there were six patients with spontaneous bacterial peritonitis, five biliary tract infection, five liver abscesses, five with pelvic inflammatory disease, one acute ruptured appendicitis and another i ...19938281491
non-sporing anaerobes in certain surgical group of patients.the magnitude of non-sparing anaerobic (nsa) infections has been defined in the postoperative wounds on colorectum in children (57.1%), general surgery (0%), abdominoperineal and uterocervical operations (11-45%) in gynaecologic and obstetrical cases and perforative peritonitis (25.8-32.3%). children below the 6 months age group bear less risk of acquiring nsa infection. under certain situations, metronidazole combats nsa infections in a better way than other antibacterials. the bacteriology of ...19958648152
characterization of saccharolytic bacteroides and prevotella isolates from infected dog and cat bite wounds in humans.saccharolytic, nonpigmented, anaerobic gram-negative rods isolated from infected dog and cat bite wounds in humans have been poorly characterized, and most are not included in the databases of kits used for anaerobic identification; thus, they are problematic for clinical laboratories to identify. fifty strains isolated from such wounds were characterized with commercial kits for preformed-enzyme detection, carbohydrate fermentation, and other biochemical tests. pcr fingerprinting was performed ...19979003606
microbial findings of deep peri-implant bone defects.the objective of this study was to examine inflammatory tissue in deep peri-implant bone pockets (> 5 mm) for anaerobic bacteria colonization. the peri-implant inflammatory tissue of bone defects from 12 edentulous patients with 18 unsuccessful implants (imz type) was removed after surgical opening of the defects. after grinding the tissue with glass beads in nutrient solution, an aliquot of the suspension was plated and incubated on appropriate culture media. the quantitative and qualitative di ...19979048462
associations of endodontic symptoms and signs with particular combinations of specific bacteria.significant associations have been reported between (a) specific bacterial species isolated from root canals and (b) between individual bacterial species and endodontic symptoms and signs. the prime objective of this study was to determine whether particular combinations of specific bacteria are associated with individual endodontic symptoms and signs. seventy root canals were investigated microbiologically taking care to maintain the viability of obligate anaerobes, which accounted for 64% of t ...19969206427
detection of dextranase-producing gram-negative oral bacteria.thirty-one strains of 23 gram-negative oral bacterial species were examined for dextran-degrading activity on agar plates containing blue dextran. one strain each of capnocytophaga ochracea, capnocytophaga sputigena, prevotella loescheii, prevotella melaninogenica and prevotella oralis had detectable dextranase activity. the culture supernatants of p. melaninogenica and p. oralis cells contained dextranases of multiple sizes, but those of the other three species had a single size of enzyme. a 56 ...19989872116
prevotella oralis homograft-valve endocarditis complicated by aortic-root abscess, intracardiac fistula, and complete heart block. 199910194101
antibiotic susceptibility of putative periodontal pathogens in advanced periodontitis patients.in the present study, the antibiotic susceptibility of most prevalent micro-organisms in advanced periodontitis patients was evaluated. in 56 patients, pooled subgingival plaque samples were taken from the deepest site of each quadrant and were cultivated anaerobically. from each patient, the 4 most frequently encountered types of bacterial colonies were subcultured and identified (rapid id 32 a). from all bacterial species identified in the 224 subcultures, the 4 most prevalent were used for su ...199910382573
oral malodor in children and volatile sulfur compound-producing bacteria in saliva: preliminary microbiological investigation.this study examined and compared levels of salivary bacteria which produced volatile sulfur compounds (vsc) in young children with and without oral malodor.199910509331
[the dynamics of the colonization by the microbial flora of the mouth of the different materials used for dental prosthetics].colonization of materials used for making dentures with oral microflora was studied in 12 patients with chronic generalized medium severe periodontitis during remission. time course of dentures colonization with representatives of oral microflora (s. sanguis, peptostreptococcus sp., prevotella oralis) and with bacteria pathogenic for the periodontium (prevotella melaninogenica, a. naeslundii, fusobacterium sp.) depends on the material from which the dentures are made. minimal colonization of bac ...200010693351
[pathogen spectrum and resistance status of exclusively anaerobic odontogenic infections].out of 440 dentogenic pyogenic infections, 171 exclusively caused by anaerobes were investigated to understand the importance of anaerobic bacteria in dental pyogenic processes better. grampositive anaerobic bacteria dominated. the predominant grampositive isolates in monoinfections were peptostreptococci and in the case of mixed infections, strains of the genus eubacterium. strains of prevotella and porphyromonas dominated the gramnegative anaerobic spectra. the resistance to penicillin was ver ...200010900958
characterization of bacterial orofacial infections using a new murine model.we devised a new murine orofacial infection model using bacteria from odontogenic infection origins and characterized the experimental infections. in this model, bacteria were injected into the submandible of mice. streptococcus constellatus and peptostreptococcus micros produced a single abscess at the injection site and their abscess-forming and lethal abilities were low: the median abscess-forming dose (af(50)) of s. constellatus and p. micros were 10(8.5-10.7)and 10(10.2-10.6)cfu/mouse, and ...200010906266
bacterial susceptibility to amoxicillin and potassium clavulanate in advanced periodontitis patients not responding to mechanical therapy.between 4 and 8% of periodontitis patients are reported to respond poorly to conventional therapy. in these cases, adjunctive use of systemic antibiotics might be a reasonable therapeutic approach. the purpose of this study was to evaluate the effects of systemic amoxicillin/clavulanate as adjunct to periodontal surgery on the predominant subgingival microorganisms in patients not responding to mechanical therapy. furthermore, the bacterial susceptibility to amoxicillin/clavulanate was analyzed ...200011073328
the effect of statherin and its shortened analogues on anaerobic bacteria isolated from the oral cavity.the susceptibility (mic) of 44 strains of anaerobic bacteria isolated from the oral cavity and 3 standard strains to statherin and its c-terminal fragments with sequences qyqqytf, yqqytf, qqytf, qytf and ytf was determined by means of plate dilution technique in brucella agar with 5% content of defibrinated sheep's blood, menadione and hemin. the culture was anaerobic. as shown, at concentrations from 12.5 to 100 microg/ml statherin and its c-terminal fragments inhibited the growth of anaerobic ...200011293657
association of early onset periodontitis microbiota with aspartate aminotransferase activity in gingival crevicular fluid.the objective of this study was to determine the relationship between the activity of the enzyme aspartate aminotransferase (ast) in gingival crevicular fluid (gcf) using the colorimetric periogard (ptm) test and the subgingival microflora in early onset periodontitis lesions.200111737506
evaluation of the rapid id 32a system for identification of anaerobic gram-negative bacilli, excluding the bacteroides fragilis group.objective: to evaluate the rapid id 32a system (biomérieux, marcy-l'etoile, france) for the identification of anaerobic gram-negative bacilli, excluding the bacteroides fragilis group. methods: five hundred and twenty-eight identified clinical isolates of non-b. fragilis group anaerobic gram-negative bacilli were tested in the rapid id 32a system, and identifications were compared with those obtained with conventional biochemical tests and gas-liquid chromatography. results: the rapid id 32a sys ...199911856276
[study of bacteriology on local application of tinidazole stilus in treatment for periodontitis].the study was designed to evaluate the antiseptic efficacy of tinidazole stilus against periodontal pathogen.200112539636
[lung abscess caused by prevotella oralis and prevotella ruminicola in a hiv-positive patient]. 200312756910
bacteroides oralis, proposed new species isolated from the oral cavity of man.loesche, w. j. (forsyth dental center and harvard school of dental medicine, boston, mass.), s. s. socransky, and r. j. gibbons. bacteroides oralis, proposed new species isolated from the oral cavity of man. j. bacteriol. 88:1329-1337. 1964.-twenty-seven strains of oral bacteroides were characterized and compared with a national collection of type cultures strain of bacteroides fragilis as well as strains of intestinal bacteroides. a combination of acid end products, gas production, pentose ferm ...196414234789
apical periodontitis development and bacterial response to endodontic treatment. experimental root canal infections in monkeys with selected bacterial strains.in six monkeys, 160 root canals were inoculated with a combination of four bacterial strains belonging to species streptococcus milleri, peptostreptococcus anaerobius, prevotella oralis, and fusobacterium nucleatum. in two other monkeys, 24 root canals were inoculated with a five-strain combination consisting of these strains and a strain of enterococcus faecalis. all strains were previously isolated from an infected monkey root canal. after 8-12 months, survival of the strains was recorded bact ...200415154917
meningitis in a dog caused by prevotella oralis.prevotella oralis, an obligate gram-negative anaerobe, was shown to be the cause of meningitis in a two-year-old crossbreed dog. the dog had a six-week history of lethargy and neck pain. on presentation, the animal was reluctant to walk, had a stiff gait and neck pain on flexion. analysis of cerebrospinal fluid documented a neutrophilic pleocytosis (7488 cells/microl) and the presence of occasional intracellular bacterial coccobacilli. the aetiological diagnosis was confirmed by routine aerobic ...200415352414
proteinase activity of prevotella species associated with oral purulent infection.prevotella intermedia and prevotella nigrescens are often regarded as principal causes of acute dentoalveolar infection; however, other species within the genus are also known to be associated with such infection. the aim of this study was to determine the in vitro proteolytic activity of these different prevotella species that have been implicated with dentoalveolar infection. a total of 234 strains were obtained from pus specimens from dentoalveolar infection and from the plaque of healthy vol ...200616586023
comparative in vitro susceptibilities of 396 unusual anaerobic strains to tigecycline and eight other antimicrobial agents.tigecycline was tested against 396 strains of lesser-known anaerobic species encountered in human infections. it was active against all gram-positive strains and 228 of 232 gram-negative anaerobes at < or =1 microg/ml. one strain of prevotella oralis was nonsusceptible at 8 microg/ml.200616940056
coaggregation interactions between oral and endodontic enterococcus faecalis and bacterial species isolated from persistent apical periodontitis.interactions between enterococcus faecalis and other species found in root canal infections might be important for the development and persistence of periapical disease. the aim of this study was to investigate the coaggregation interactions between e. faecalis clinical isolates and species previously shown to survive and induce apical periodontitis in monkeys: peptostreptococcus anaerobius, prevotella oralis, fusobacterium nucleatum, and streptococcus anginosus. intergeneric coaggregation assay ...200616982270
in vitro antimicrobial activity of propolis samples from different geographical origins against certain oral pathogens.propolis is an agent having antimicrobial properties, however, its composition can vary depending on the area where it is collected. in the present study, the antimicrobial activity of five propolis samples, collected from four different regions in turkey and from brazil, against nine anaerobic strains was evaluated. ethanol extracts of propolis (eep) were prepared from propolis samples and we determined minimum inhibitory concentrations (mic) and minimum bactericidal concentrations (mbc) of eep ...200717475517
moxifloxacin resistance is prevalent among bacteroides and prevotella species in greece.moxifloxacin is recommended in the empirical treatment of infections involving gram-negative anaerobes. however, current european data regarding its activity against anaerobic pathogens are limited. in order to evaluate its potency, we comparatively studied the activity of moxifloxacin against recently isolated gram-negative anaerobes.200818385145
Displaying items 1 - 100 of 123