| development and characterization of porphyromonas gingivalis-specific rat t-cell clones. | porphyromonas gingivalis has been implicated as a major pathogen in periodontitis. to determine the role of t cells in the regulation of this disease, a method was developed for the generation and characterization of rat t-cell clones with antigen specificity to p. gingivalis whole cells. the clones studied so far demonstrated a t-helper (th) phenotype w3/13+, w3/25+, ox8- and ox22-. these t-cell clones proliferated in vitro in response to p. gingivalis, but not to other bacteria (prevotella int ... | 1992 | 1281976 |
| antibacterial activity of dental implant metals. | this study was conducted to investigate the specific in vitro antibacterial effect of seven dental implant metals on oral bacteria which have often been identified in failing implants. the metals chosen for evaluation were titanium, chromium, cobalt, aluminum, iron, gold, and vanadium. these metals were selected because they are contained in many commonly used dental implants. the bacteria selected for this study included isolates of porphyromonas endodontalis, porphyromonas gingivalis, prevotel ... | 1992 | 1288798 |
| putative periodontal pathogens on titanium implants and teeth in experimental gingivitis and periodontitis in beagle dogs. | the microflora around titanium implants and teeth in 4 beagle dogs was analyzed in order to follow the longitudinal development from healthy conditions to experimental gingivitis and periodontitis. a 2-month plaque control program was performed to establish healthy conditions on titanium implants and control teeth (baseline, day 0). subgingival bacterial samples and radiographs were obtained. the plaque control was ceased and all measurements were repeated on day 21 (gingivitis). a new period of ... | 1992 | 1290791 |
| phagocytosis of porphyromonas gingivalis and prevotella intermedia by human polymorphonuclear leukocytes in clots formed from human plasma or purified fibrinogen. | we have studied the polymorphonuclear leukocyte (pmn) phagocytosis of porphyromonas gingivalis (bacteroides gingivalis) and prevotella intermedia (bacteroides intermedius) in three-dimensional fibrin meshworks formed by either clotting human plasma or by clotting purified fibrinogen with human serum incorporated. pmn phagocytosis of rhodamine isothiocyanate-labelled p. gingivalis in the fibrinogen clot was 43 +/- 6% and in the plasma clot was 62 +/- 10%; for p. intermedia the values were 15 +/- ... | 1992 | 1312590 |
| gingival crevicular fluid concentration and side effects of minocycline: a comparison of two dose regimens. | the purpose of this study was to conduct a direct comparison of two dose regimens of minocycline to determine 1) whether they achieved crevicular fluid concentrations in a therapeutic range; and 2) the frequency of side effects. in a double-blind design, 30 patients divided into 2 groups were given either 100 mg minocycline or 200 mg minocycline per day for an 8-day period. the concentration of minocycline in the gingival clevicular fluid (gcf) at 8 days was 4.77 micrograms/ml for the 100 mg a d ... | 1992 | 1313100 |
| humoral antibody responses in periodontal disease. | several forms of periodontal disease are considered to be infectious diseases with associated specific bacteria. this study examined the humoral antibody levels as assayed by elisa to actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and prevotella intermedia in adult periodontitis (ap), localized juvenile periodontitis (ljp), rapidly progressive periodontitis (rpp), and in periodontally healthy subjects (hs). sixty-two of the 64 (96.9%) patients had significantly elevated antibody ... | 1992 | 1313106 |
| evaluation of oral bacteria as risk indicators for periodontitis in older adults. | the prevalence of people and sites with attachment loss, pocket depth, actinobacillus actinomycetemcomitans, prevotella intermedia, and porphyromonas gingivalis are described for a random sample of 366 black and 297 white community-dwelling adults, aged 65 or over, residing in five counties in north carolina. in addition, relationships between sites harboring these microorganisms and loss of attachment (la) and pocket depth (pd) are presented in a manner that considers the lack of independence o ... | 1992 | 1313107 |
| microbiology of healthy and diseased periodontal sites in poorly controlled insulin dependent diabetics. | a group of poorly-controlled insulin dependent diabetes mellitus (iddm) patients were examined in a cross-sectional design for total microbial levels, microbial incidence, and the percent levels of selected periodontal microorganisms. these organisms were selected on the basis of prior reports that associated them with either periodontal disease or health. one periodontally-healthy and one periodontally-diseased site were examined in each iddm patient. increased levels of the periodontal pathoge ... | 1992 | 1315389 |
| the occurrence of periodontitis-related microorganisms in relation to titanium implants. | the peri-implant space was examined for the presence of periodontitis-related microorganisms in 19 patients. all patients had been fitted with osseointegrated titanium implants which were clinically sound at the time of examination and all had been prescribed daily 0.2% chlorhexidine mouthrinse. in 17 patients, two contralateral fixtures in patients were selected, the remaining 2 patients had only one fixture, and associated mucosal clinical variables assessed for plaque accumulation, gingival i ... | 1992 | 1317427 |
| gene cloning of porphyromonas gingivalis specific antigens recognized by serum of adult periodontitis patient. | 1. porphyromonas gingivalis is believed an important pathogen of adult periodontitis. a gene library of p. gingivalis 381 was constructed in lambda phage vector l47.1. the library was probed with serum obtained from patients of severe adult periodontitis. two clones, lambda mdbg101 and lambda mdbg103 which were expressed, 200 and 160 kda respectively, were selected and further studied. 2. the expressed antigens in these two clones were also reacted with rabbit antiserum against whole cells, caps ... | 1992 | 1319357 |
| e-test: a new technique for antimicrobial susceptibility testing for periodontal microorganisms. | the purpose of the study was to validate the epsilometer test (e-test) method for antimicrobial susceptibility testing of selected periodontopathic microorganisms using the agar dilution method as a standard. the e-test has been developed to provide a direct quantification of antimicrobial susceptibility of microorganisms. the device consists of a predefined, continuous, and exponential gradient of antibiotic concentrations immobilized along a rectangular plastic test strip. after 48 hours incub ... | 1992 | 1324301 |
| development of a rapid latex agglutination test for periodontal pathogens. | the studies reported here describe the development, characterization, and initial application of latex agglutination assays for periodontal pathogens. latex reagents were prepared by sensitization of latex microspheres with rabbit igg antibodies to either actinobacillus actinomycetemcomitans, porphyromonas gingivalis, or prevotella intermedia. the protein concentration utilized for sensitization and microsphere size were optimized. one reagent was prepared to a. actinomycetemcomitans and a secon ... | 1992 | 1324302 |
| a preliminary study on the effects of the nd:yag laser on root surfaces and subgingival microflora in vivo. | the purpose of this study was to examine the effects of root preparation using the pulsed nd:yag laser, either alone or in combination with manual instrumentation. study specimens consisting of 18 teeth with associated periodontal pockets from 8 different patients were treated as follows: 5 specimens were root planed with curets followed by laser exposure for 3 minutes using energy settings of 3.0 w at 20 pps; 2 specimens were root planed and then laser treated for 3 minutes using settings of 2. ... | 1992 | 1324305 |
| inhibition of peptidase and glycosidase activities of porphyromonas gingivalis, bacteroides intermedius and treponema denticola by plant extracts. | aqueous extracts from 5 plants used widely in kenya as chewing sticks (mswaki) for the control of oral hygiene were tested for their ability to inhibit extracellular peptidase and glycosidase enzyme activities produced by the periodontopathic bacteria porphyromonas gingivalis (formerly bacteroides gingivalis), bacteroides intermedius and treponema denticola. the plants studied were rhus natalensis, cupressus hisitanica, sida cordifolia, olea africana and euclea divinorum. protease activities, in ... | 1992 | 1325483 |
| microbial etiology of periodontal diseases. where are we? where are we going? | many studies indicate a strong association of actinobacillus actinomycetemcomitans with localized juvenile periodontitis. species associated with adult periodontitis include bacteroides forsythus, porphyromonas gingivalis, prevotella intermedia, a. actinomycetemcomitans, and wolinella recta. capnocytophaga species may be important in pubertal gingivitis. an unnamed spirochete related to treponema pallidum has been identified in acute necrotizing ulcerative gingivitis lesions. species isolated fr ... | 1992 | 1325846 |
| lipopolysaccharides of bacteroides intermedius (prevotella intermedia) and bacteroides (porphyromonas) gingivalis induce interleukin-8 gene expression in human gingival fibroblast cultures. | lipopolysaccharides (lps) prepared from bacteroides intermedius (prevotella intermedia) and bacteroides (porphyromonas) gingivalis by hot phenol-water extraction induced interleukin-8 (il-8) mrna in normal human gingival fibroblast cultures, as demonstrated by northern (rna) blot analysis. il-8 mrna levels began to increase after a 2-h exposure, reached a maximum after 12 h, and then dropped to the unstimulated level at 48 h. il-8 mrna levels were also enhanced in a dose-dependent manner. by con ... | 1992 | 1328062 |
| occurrence of periopathogens in smoker and non-smoker patients. | the aim of the present study was to elucidate the interrelationship between cigarette smoking and the occurrence of periopathogens, i.e., actinobacillus actinomycetemcomitans, bacteroides gingivalis and bacteroides intermedius. the study was based on 145 patients with clinically severe periodontitis, 52 men (age range 32-73 years) and 93 women (age range 32-74 years). 83 patients were smokers (> or = 15 cigarettes/day) and 62 non-smokers. bacterial samples were collected from one site with a pro ... | 1992 | 1331203 |
| comparative clinical and microbiological effects of topical subgingival application of metronidazole 25% dental gel and scaling in the treatment of adult periodontitis. | the aim of the study was to compare the clinical and microbiological effects of topical application of a metronidazole gel and a single session of subgingival scaling in the treatment of adult periodontitis. an open, randomized controlled clinical study design was employed. each of 24 subjects received the 2 treatments simultaneously each in 2 randomly selected quadrants of the dentition. the metronidazole, 25% gel was applied subgingivally on days 0 and 7. scaling was carried out in one quadran ... | 1992 | 1332988 |
| bacterial concentration fluorescence immunoassay (bcfia) for the detection of periodontopathogens in plaque. | a bacterial concentration fluorescence immunoassay (bcfia) was developed to rapidly detect periodontopathic bacteria in human plaque samples. the bcfia utilized fluorescent-tagged monoclonal antibodies (mabs) directed against the lipopolysaccharide of selected gram-negative bacteria. microorganisms identified in plaque using the bcfia included actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, and fusobacterium nucleatum. the immunoassay procedure involved com ... | 1992 | 1336047 |
| putative periodontopathogens in "diseased" and "non-diseased" persons exhibiting poor oral hygiene. | the aim of the study was to assess the occurrence of some putative periodonto-pathogens in "test" and "control" sites in "diseased" and "non-diseased" persons, respectively, from an adult rural kenyan population exhibiting poor oral hygiene and widespread loss of attachment (la). 14 persons (less than 35 years) were assigned to a "diseased" category on the basis of at least 4 sites with la greater than or equal to 4 mm; at least 5 mm la and a pocket greater than or equal to 4 mm interproximally ... | 1992 | 1346265 |
| heterogeneity of surfaces of subgingival bacteria as detected by zeta potential measurements. | porphyromonas gingivalis, prevotella intermedia, and actinobacillus actinomycetemcomitans (a.a.) are gram-negative bacteria which are implicated in various forms of periodontal disease. the gram-positive peptostreptococcus micros may also play an important role. for investigation of the possible adhesion and colonization mechanisms of these organisms, the charge properties of the outermost layers of bacterial cell surfaces were studied through the measurement of zeta potentials at various ph val ... | 1992 | 1357016 |
| biochemical and chemical studies on strains designated prevotella intermedia and proposal of a new pigmented species, prevotella nigrescens sp. nov. | a total of 31 strains of prevotella intermedia were subjected to dna-dna hybridization and were characterized by performing physiological tests and by performing a multilocus enzyme analysis, using malate dehydrogenase and glutamate dehydrogenase. all of the strains assigned to p. intermedia fermented glucose and sucrose, hydrolyzed starch but not esculin, and produced indole, acetic, isobutyric, isovaleric, and succinic acids as metabolic end products. the results of dna reassociation experimen ... | 1992 | 1390107 |
| identification of proteases from periodontopathogenic bacteria as activators of latent human neutrophil and fibroblast-type interstitial collagenases. | activation of latent human fibroblast-type and neutrophil interstitial procollagenases as well as degradation of native type i collagen by supra- and subgingival dental plaque extracts, an 80-kda trypsinlike protease from porphyromas gingivalis (atcc 33277), a 95-kda chymotrypsinlike protease from treponema denticola (atcc 29522), and selected bacterial species commonly isolated in periodontitis was studied. the bacteria included were prevotella intermedia (atcc 25261), prevotella buccae (es 57) ... | 1992 | 1398963 |
| growth of porphyromonas gingivalis, treponema denticola, t. pectinovorum, t. socranskii, and t. vincentii in a chemically defined medium. | a chemically defined medium, omiz (oral microbiology and immunology, zürich)-w1 was developed. medium omiz-w1 supports the long-term proliferation of a wide range of oral anaerobes, including representative strains of four treponema species and porphyromonas gingivalis. high concentrations of ascorbic acid and ammonium ions proved to be important for the growth of these organisms. t. denticola cd-1 grew in the absence of polyamines and long-chain fatty acids, t. pectinovorum and t. socranskii re ... | 1992 | 1400984 |
| antibodies in normal and diseased pulps reactive with microorganisms isolated from deep caries. | immunoglobulin molecules in the supernatant fluids (sf) from pulpal explant cultures have been observed to react with microorganisms implicated in infections of root canals. in this study, the reactivity of immunoglobulin molecules in the sf from normal and irreversible pulpitis pulps to six strains of predominant microorganisms isolated from the immediate layer of carious lesions above the pulps used for explant cultures was investigated using an enzyme-linked immunosorbent assay. two atcc stra ... | 1992 | 1402561 |
| laminin binding to prevotella intermedia. | the interaction of laminin (lm), a basement membrane protein abundant in the periodontium, with 66 strains of prevotella intermedia isolated from diseased pockets, was tested in a 125i-labeled protein binding assay. the mean binding value was 28% of the total protein added. the binding significantly increased to 35% when the environmental ph decreased from 7 to 6. the lm interaction was characterized in a highly binding (about 65%) strain, omgs105. the binding was rapid and required about 1 min ... | 1992 | 1408358 |
| experimentally induced infection by oral anaerobic microorganisms in a mouse model. | a mouse model was developed to study the abscessogenic potential of pure and mixed cultures of oral anaerobes associated with infections of endodontic origin. maximum subcutaneous lesions were seen on their backs between 3 and 14 d after inoculation. strains of fusobacterium nucleatum, peptostreptococcus anaerobius, and veillonella parvula were pathogenic in pure culture. a mixed culture of f. nucleatum with either porphyromonas gingivalis or prevotella intermedia was significantly more pathogen ... | 1992 | 1408362 |
| the effects of interferon-gamma and bacterial lipopolysaccharide on cd14 expression in human monocytes. | cd14 has been reported to be the lipopolysaccharide (lps)-lps binding protein receptor. the effects of interferon-gamma (ifn-gamma) on cd14 expression have not been clearly established. the purpose of this investigation was to examine the effects of ifn-gamma alone and ifn-gamma followed by bacterial lps on cd14 expression. human peripheral blood monocytes were isolated by counterflow centrifugal elutriation (cce). monocytes were cultured for 48 h with ifn-gamma alone or for 24 h with ifn-gamma ... | 1992 | 1431309 |
| phenolic constituents in erythrina x bidwilli and their activity against oral microbial organisms. | five flavonoid compounds, including two new isoflavanones, were isolated from the root bark of erythrina x bidwilli. their structures were determined to be 2,10-di(gamma,gamma-dimethylallyl)-3,9-dihydroxypterocarpan (erythrabyssin ii), 6,8-di(gamma,gamma-dimethylallyl)-7,2',4'-trihydroxyisoflavanone (bidwillon a), 8-gamma,gamma-dimethylallyl-2',4'-dihydroxy-[6",6"-di-methylpyrano - (2",3":7,6)]isoflavanone (bidwillon b), 8-gamma,gamma-dimethylallyl-7,4'-dihydroxyisoflavone (8-gamma,gamma-dimethy ... | 1992 | 1464104 |
| experimental studies of survival of anaerobic bacteria at 4 degrees c and 22 degrees c in two different transport systems. | the survival of anaerobic bacteria on swabs in two different transport systems at 4 degrees c and 22 degrees c was evaluated. the transport systems were a charcoal-impregnated cotton swab in modified stuart transport medium (mst), and a viscose swab in modified siff transport medium (btm) (bionor). the following eight clinical strains of anaerobic bacteria were tested for quantitative recovery at 24 h, 48 h, 72 h, and 96 h; fusobacterium necrophorum, bacteroides melaninogenicus, bacteroides inte ... | 1992 | 1472363 |
| susceptibility of anaerobic microorganisms to hypothiocyanite produced by lactoperoxidase. | the susceptibility of capnocytophaga ochracea, eikenella corrodens, eubacterium yurii, fusobacterium nucleatum, peptostreptococcus micros, prevotella intermedia, selenomonas sputigena, wolinella recta to hypothiocyanite (oscn-) produced by the lactoperoxidase system was tested. results showed a decrease of bacterial survival rate after oscn- exposure, with an intra- and inter-species variability from 0 to 95% for c. ochracea, 34-100% for e. corrodens, 0-83% for e. yurii, 1-15% for f. nucleatum, ... | 1992 | 1481764 |
| associations between microbial species in dental root canal infections. | the existence of commensal or antagonistic relationships between microorganisms in the root canals of teeth with apical periodontitis was investigated. samples were taken from 65 infected human root canals and were analysed according to species, frequency of occurrence and proportion of the total isolated flora. the most frequent species were fusobacterium nucleatum, prevotella intermedia, peptostreptococcus micros, peptostreptococcus anaerobius, eubacterium alactolyticum, eubacterium lentum and ... | 1992 | 1494447 |
| periodontal pathogens in the shallow pockets of immigrants from developing countries. | the aim of this study was to examine the distribution of typical periodontitis-associated microorganisms in refugees arriving from non-industrialized countries, and to relate the presence of these organisms to the periodontal condition of the subjects. thirty males between 35-44 years of age were surveyed. dental plaque, calculus, gingivitis, loss of attachment, and probing depths were recorded for all surfaces. a total of 90 microbiological samples were taken with paper points from mesial sites ... | 1992 | 1494449 |
| relationship between oral gram-negative anaerobic bacteria in saliva of the mother and the colonization of her edentulous infant. | various gram-negative anaerobic bacterial species can be detected in the oral flora of edentulous infants. however, knowledge of the initial infection source is still scarce. thirty caucasian mothers (mean age 30 years) and their edentulous infants (mean age 3 months) were examined for the possible similarity of the oral gram-negative anaerobic flora. paraffin-stimulated saliva was collected from the mothers. a pooled swab sample from mucosal surfaces and unstimulated saliva were collected from ... | 1992 | 1494450 |
| subgingival distribution of periodontal pathogenic microorganisms in adult periodontitis. | the association between specific plaque microorganisms and periodontal diseases has been the subject of much recent interest due to its potential importance in the diagnosis and classification of these diseases. in order to optimize microbiological tests in periodontal therapy, it is important to know how many subgingival plaque samples must be assayed from a single patient in order to ascertain infection with a periodontal pathogen. to answer this question the present study assessed the distrib ... | 1992 | 1527685 |
| phenotypic characteristics and dna relatedness in prevotella intermedia and similar organisms. | we have recently isolated from the gingival pockets, periapical lesions and saliva some anaerobic gram-negative, black-pigmented rods. many of these isolates exhibited phenotypic characteristics similar to prevotella intermedia (bacteroides intermedius). however, several of these isolates, although resembling p. intermedia in most of the phenotypic expressions, were capable of fermenting lactose, a biochemical characteristic atypical of p. intermedia. these atypical clinical isolates (strains ca ... | 1992 | 1528627 |
| serum igg reactive with oral anaerobic microorganisms associated with infections of endodontic origin. | numerous species of bacteria have been implicated with infections of endodontic origin. the purpose of this study was to compare the levels of serum igg antibodies reactive with a panel of 10 oral anaerobic microorganisms implicated in infections of endodontic origin. serum samples were collected from 4 patient groups that included healthy patients without endodontic or periodontal disease, patients with chronic adult periodontal disease, patients with endodontic disease and patients with combin ... | 1992 | 1528630 |
| comparative mitogenicity and polyclonal b cell activation capacity of eight oral or nonoral bacterial lipopolysaccharides in cultures of spleen cells from athymic (nu/nu-balb/c) and thymic (balb/c) mice. | optimal stimulatory doses of purified phenol-water extracted lipopolysaccharides (lps) from 5 selected strains of 3 putative periodontopathogens (fusobacterium nucleatum, prevotella intermedia, and veillonella), 3 strains of 2 nonoral bacterial species (bacteroides fragilis and salmonella enteritidis), and pokeweed mitogen (pwm) induced significantly higher maximum mitogenic responses and polyclonal ig production in cultures of unfractionated spleen cells from nu/nu-balb/c (nude) than from balb/ ... | 1992 | 1528635 |
| [effect of ribonuclease on cell cycle dynamics in tumor cells in vitro and in vivo]. | the effects of rnaase from bacteroides intermedius and pancreatic rnaase on the dynamics of cellular cycle phases were studied in the cells of ascitic lympholeukemia nk/ly in vivo and in vitro, in the cells of the human ovary carcinoma caov and in the cells of ascitic leukemia p388 in vivo. it was shown that both the rnaases induced cell accumulation (blocking) in the g2/m phase of a cellular cycle with simultaneous certain depletion of the proportion of dna-synthesizing cells (s-phase). in in v ... | 1992 | 1530355 |
| serial doxycycline and metronidazole in prevention of recurrent periodontitis in high-risk patients. | the efficacy of metronidazole and doxycycline in preventing recurrent periodontitis was studied in 23 patients. after treatment in the previous 7 months with either bimonthly scaling and 3 weeks of systemic doxycycline (11 subjects) or scaling and placebo (12 subjects), patients were monitored for recurrent periodontitis and were scaled every 2 months. when either a periodontal abscess or greater than 2 mm loss of gingival attachment was observed, metronidazole was administered (250 mg every 8 h ... | 1992 | 1552469 |
| physicochemical and structural investigation of the surfaces of some anaerobic subgingival bacteria. | the surfaces of nine clinical isolates of porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetemcomitans, and peptostreptococcus micros and that of laboratory strain p. gingivalis w83 were studied by using contact angle measurements, x-ray photoelectron spectroscopy, infrared spectroscopy, microelectrophoresis of whole cells, and transmission electron microscopy of whole and sectioned cells. p. intermedia strains were hydrophilic, as judged from their small water contact a ... | 1992 | 1599251 |
| analysis of site specific periodontal bacteria sampling schemes. | a great deal of controversy has existed in the periodontal literature as to whether the site or the subject should be the unit of analysis. using the site as the unit of analysis assumes that observations of sites within the same subject are independent and ignores between subject variation. the purpose of this report is to evaluate the influence that the unit of analysis has on estimating the number of necessary site specific bacterial samples from each subject. the number of bacterial samples ... | 1992 | 1625150 |
| early effects of periodontal therapy on the detection frequency of four putative periodontal pathogens in adults. | this study aimed to examine early posttreatment changes in the periodontal microflora. paper point sampling and conventional bacterial cultivation were used to monitor the effects of surgical and non-surgical periodontal therapy on the detection frequency of porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetemcomitans, and capnocytophaga species in deep periodontal pockets. ten patients, 5 men and 5 women (mean age 44 years), with advanced periodontal disease were select ... | 1992 | 1625154 |
| classification of oral pigmented anaerobic bacilli by pyrolysis mass spectrometry and biochemical tests. | clinical (66) and reference (5) strains of pigmented gram-negative anaerobic bacilli, identified as prevotella intermedia (47), pr. melaninogenica (1), pr. corpora (8), porphyromonas asaccharolyticus (12), p. endodontalis (1) and p. gingivalis (2), were examined by pyrolysis mass spectrometry (pms) and in conventional tests. numerical classification based on conventional test reaction patterns (ctrps) resolved five clusters, four comprising strains identified as pr. intermedia, pr. corpora, pr. ... | 1992 | 1625317 |
| microflora and bacterial dna restriction enzyme analysis in young adults with periodontitis. | as a result of an epidemiological survey on the periodontal status of young adults, a school was found with a relatively high number of subjects showing loss of attachment (18 out of 87). in 10 of these subjects the microflora of one pocket and of the dorsum of the tongue was investigated with special reference to black-pigmented species and actinobacillus actinomycetemcomitans. prevotella intermedia was the most prevalent species, being found in 9 patients. a. actinomycetemcomitans was isolated ... | 1991 | 1645404 |
| variance in recovery of periodontitis-associated bacteria caused by sampling technique and laboratory processing. | the influence of sampling procedure and of laboratory processing on the recovery of bacteroides gingivalis, bacteroides intermedius, and actinobacillus antinomycetemcomitans from periodontitis sites was evaluated. thirty-three adult subjects with severe periodontitis participated in the study. in all, 462 samples from 81 sites were examined. the samples were taken using the paper point technique. the cultivations were performed by use of enriched brucella agar for determination of total colony-f ... | 1991 | 1658714 |
| systemic antibody responses to oral microorganisms in the cynomolgus monkey: development of methodology and longitudinal responses during ligature-induced disease. | systemic antibody responses to oral microorganisms were studied during ligature-induced periodontal disease in a non-human primate (nhp) model. methodology was developed using elisa techniques to assess total igg and igm levels in the serum from the nhp. in addition, an elisa was developed utilizing affinity-purified anti-human isotype reagents to detect nhp serum antibody responses to porphyromonas gingivalis, actinobacillus actinomycetemcomitans, prevotella intermedia and fusobacterium nucleat ... | 1991 | 1665573 |
| inhibitory effect of stannous fluoride and other commonly used antimicrobial agents on oral bacteria. | the purposes of this investigation were to evaluate and compare the antimicrobial effect of stannous fluoride (snf2) gel and other commonly used oral antimicrobial agents/products. the antibacterial inhibitory effect of the various agents was evaluated by their effect against oral plaque bacteria including strains of streptococcus sanguis, streptococcus sobrinus, actinomyces viscosus, actinobacillus actinomycetemcomitans, bacteroides gingivalis and bacteroides intermedius. a lawn of the specific ... | 1991 | 1681003 |
| stimulation of lymphocytes in vitro by bacteroides intermedius and bacteroides (porphyromonas) gingivalis sonicates. | the present study was designed to assess whether the in vitro stimulation of lymphocytes by sonicates of bacteroides intermedius and bacteroides (porphyromonas) gingivalis is antigen specific or non-specific. in addition, the role of t and b lymphocytes in these responses was assessed. peripheral blood lymphocytes obtained from healthy volunteers were cultured in the presence of these bacterial preparations and the proliferative response was measured. in similar experiments the response of umbil ... | 1990 | 1691286 |
| the relationship of serum igg antibody titers to periodontal pathogens to indicators of the host response in crevicular fluid. | in this study; the relationship of indicators of the local host response in gingival crevicular fluid (gcf) to the serum antibody titer to periodontal pathogens was examined. 15 patients with chronic adult periodontitis were studied. gcf was collected and analyzed for the total amount of igg, igm, the lysosomal enzyme b-glucuronidase (bg) and alpha-2-macroglobulin (alpha 2m). at the same examination, serum from these patients was collected, and enzyme-linked immunosorbent assays used to determin ... | 1990 | 1696949 |
| human lactoferrin binding to porphyromonas gingivalis, prevotella intermedia and prevotella melaninogenica. | human isolates of porphyromonas gingivalis (n = 16), prevotella intermedia n = 82) and prevotella melaninogenica (n = 18) from diseased periodontal pockets were examined for interaction with human lactoferrin (hlf) in a standardized 125i-labeled protein binding assay. the highest hlf binding was found in p. intermedia strains, followed by p. gingivalis and p. melaninogenica. further characterization of the interaction was performed with 1 representative strain from each species. hlf binding to p ... | 1991 | 1726544 |
| [chronic conjunctivitis caused by oral anaerobic germs]. | a patient with chronic, unilateral conjunctivitis is presented. the predominant bacterial flora consisted mainly of prevotella intermedia (formerly bacteroides intermedius) and peptostreptococcus micros. restriction endonuclease fingerprinting of genomic dna demonstrated that p. intermedia most probably originated from the oral cavity. in the oral cavity this species was recovered from the dorsum of the tongue, the tonsillar area and the periodontal pockets. topical treatment with various antibi ... | 1991 | 1758517 |
| fluorescence immunoassay for detecting periodontal bacterial pathogens in plaque. | a particle concentration fluorescence immunoassay has been modified into a bacterial concentration fluorescence immunoassay (bcfia) to rapidly detect periodontopathic bacteria in human plaque samples. the bcfia utilizes fluorescently tagged monoclonal antibodies (mabs) directed against the lipopolysaccharide of selected gram-negative plaque bacteria. microorganisms closely associated with periodontal disease that can be identified in plaque with the bcfia include porphyromonas gingivalis, bacter ... | 1991 | 1761686 |
| rapid presumptive identification of black-pigmented gram-negative anaerobic bacteria by using 4-methylumbelliferone derivatives. | a rapid method for presumptive identification of black-pigmented gram-negative anaerobic rods was developed. using filter paper spot tests for indole production, sialidase, alpha-glucosidase, beta-glucosidase, alpha-fucosidase, and trypsinlike enzyme activities, 100% of porphyromonas gingivalis, prevotella intermedia, and bacteroides levii and 89% of prevotella corporis isolates were correctly identified to the species level. porphyromonas asaccharolytica and porphyromonas endodontalis could not ... | 1991 | 1774320 |
| biological effects of veillonella parvula and bacteroides intermedius lipopolysaccharides. | a comparative study on the endotoxic effects of lipopolysaccharide (lps) from veillonella parvula atcc 10790 and from bacteroides intermedius bmh was performed using an in vivo approach in the c57bl/6 mouse. phenol-water extracted lps of such anaerobes was purified by ultracentrifugation and dnase/rnase digestion, and characterized by a metachromatic assay for endotoxins and by electrophoresis on sds-polyacrylamide gel and silver staining. mouse ld50 for v. parvula lps was 1.479 mg and for b. in ... | 1991 | 1775087 |
| [subgingival microflora in children of early childhood, school age and circumpuberty. the proportion and frequency of gram-negative bacteria in periodontally healthy and gingivitis groups]. | the present study characterized the microbial profiles of the gingival sulci in children. subgingival samples from 36 gingivitis lesions of 18 patients and 36 sites in 18 healthy persons were examined. the tested individuals were divided into three stages according to physiological maturation i.e. early childhood, school age, circumpuberty. puberty was confirmed through examination of wrist radiographs. using continuous anaerobic techniques, samples were dispersed, diluted and then inoculated on ... | 1991 | 1784868 |
| [bacteriological examination of infections in the field of oral surgery]. | etiology of bacterial infections in the field of oral surgery was studied. a total of 270 samples collected from patients with encapsulated abscess in their oral cavities was examined and bacteria were isolated from the 244 samples (90.4%). the following results were found; 1) organisms more than one from one sample were frequently isolated from cases with parodontitis, pericoronitis and gnathitis. isolation of anaerobic bacteria was common (54.2%). 2) streptococcus milleri and streptococcus san ... | 1991 | 1791332 |
| comparative in vitro activity of cefpodoxime against anaerobes other than bacteroides fragilis. | to assess the in vitro activity of cefpodoxime against anaerobic respiratory tract and oropharyngeal pathogens 77 strains belonging to 18 gram-negative and 7 gram-positive species were studied by means of agar dilution tests. for comparison cefuroxime, amoxicillin, amoxicillin + clavulanic acid and clindamycin were also tested. cefpodoxime was found to be active at concentrations of less than or equal to 0.125 mg/l against prevotella oralis, prevotella buccalis, prevotella bivia, porphyromonas a ... | 1991 | 1800380 |
| adherence to experimental pellicle of rough-type lipopolysaccharides from subgingival plaque bacteria. | the adherence of lipopolysaccharides (lpss) from periodontal disease-associated bacteria to saliva-coated hydroxyapatite (s-ha) and serum-coated ha beads was examined by chromogenic limulus activity (toxicolor test). phenol-water extracted lps preparations from bacteroides intermedius, fusobacterium nucleatum, actinobacillus actinomycetemcomitans, eikenella corrodens, and rough-type lps from escherichia coli adhered to s-ha and serum-coated beads and agglutinated human erythrocytes. the adhered ... | 1991 | 1812466 |
| antimicrobial activity of flurbiprofen and ibuprofen in vitro against six common periodontal pathogens. | nonsteroidal antiinflammatory drugs (nsaids) such as flurbiprofen and ibuprofen, have been shown to inhibit the inflammation and alveolar bone loss associated with chronic destructive periodontal disease. however, the direct effect of nsaids on the gingival crevice microflora has not been studied. the purpose of this investigation was to evaluate the antimicrobial activity of ibuprofen and flurbiprofen in vitro on six commonly isolated periodontal pathogens. the bacterial strains evaluated were ... | 1991 | 1812907 |
| production of trypsin and chymotrypsin inhibitors by oral bacterial isolates. | using an agar-skim milk gel method, over 300 oral bacterial isolates were tested for their ability to produce trypsin or chymotrypsin inhibitor. eleven bacterial strains, including seven isolates identified as bacteroides intermedius, were found to inhibit both proteolytic activities. the inhibitory factors of the 11 bacterial strains could be divided into two categories: i) heat-stable and dialysable, and ii) heat-labile and non-dialysable. the protease inhibitor activity of b. intermedius stra ... | 1991 | 1832450 |
| multicenter evaluation of tetracycline fiber therapy. iii. microbiological response. | in a multicenter study of the effects of tetracycline (tc) fiber therapy, subgingival plaque samples were tested for 6 probable periodontal pathogens by dna probe analysis. levels of actinobacillus actinomycetemcomitans, eikenella corrodens, fusobacterium nucleatum, porphyromonas (bacteroides) gingivalis, prevotella intermedia (bacteroides intermedius), and wolinella recta were quantitatively determined in samples taken at baseline, and immediately after tc fiber removal, control fiber removal, ... | 1991 | 1832457 |
| tetracycline fiber therapy monitored by dna probe and cultural methods. | oligonucleotide dna probe and selective cultural methods were compared in their ability to monitor 6 putative periodontal pathogens in a study evaluating local tetracycline fiber therapy. subgingival plaque was sampled from 4 sites in each of 20 subjects. samples were taken before and after therapy from sites assigned to the following test groups: tetracycline (tc) fiber, scaling and root planing, control fiber, and untreated. each sample was analyzed by both dna probe and cultural methods. tota ... | 1991 | 1832458 |
| cd4 to cd8 ratio and in vitro lymphoproliferative responses during experimental gingivitis in pregnancy and post-partum. | the absolute numbers and percentages of peripheral t, b, and nk cells were assessed in 7 women, both during the second trimester of pregnancy and 6 months post-partum. furthermore, the in vitro responses of peripheral blood lymphocytes (pbl) to several mitogens and a preparation of prevotella intermedia were compared in a period of experimentally-induced gingivitis during pregnancy and post-partum. clinically, the periodontal pocket bleeding index (ppbi) was found to be higher during pregnancy t ... | 1991 | 1836497 |
| incidence of periodontitis recurrence in treated patients with and without cultivable actinobacillus actinomycetemcomitans, prevotella intermedia, and porphyromonas gingivalis: a prospective study. | a total of 98 adults previously treated for moderate to advanced periodontitis and on a trimonthly recall schedule were screened for the presence of critical levels of actinobacillus actinomycetemcomitans, prevotella (bacteroides) intermedia, and porphyromonas (bacteroides) gingivalis. patients with at least 2 positive sites were placed in a positive group and patients without or with low levels of these bacteria in a negative group. during the 30-month study the incidence of disease recurrence ... | 1991 | 1870068 |
| the stability of outer-membrane protein and antigen profiles of a strain of bacteroides intermedius grown in continuous culture at different ph and growth rates. | the stability of the outer-membrane proteins and antigens of a strain of bacteroides intermedius (vpi 8944 group genotype ii) grown in continuous culture at varying ph and growth rates (d = 0.025-0.2 h-1, ph 6.0-7.3) has been measured. the membranes showed nine major proteins (greater than 67-19.55 kilodaltons) and six major antigens (65-28 kilodaltons). membrane proteins and antigens were stable under the conditions tested; the major proteins were detected in all membranes, and the antigen prof ... | 1991 | 1878815 |
| chronic conjunctivitis caused by oral anaerobes and effectively treated with systemic metronidazole plus amoxicillin. | in this study, we report on a case of refractory, unilateral anaerobic conjunctivitis. the predominant anaerobic flora consisted of prevotella intermedia (formerly bacteroides intermedius) and peptostreptococcus micros. by using the technique of restriction endonuclease fingerprinting of genomic dna, it was shown that the p. intermedia likely originated from the oral cavity. topically applied antibiotics had failed to suppress the infection in the past. successful treatment was achieved after sy ... | 1991 | 1890173 |
| the use of genomic dna fingerprinting in studies of the epidemiology of bacteria in periodontitis. | recent studies of microbial epidemiology emphasizing the genetic organization and distribution of organisms associated with orofacial infections have led to new insights into the possible origins of pathogenicity. studies into genetic heterogeneity, acquisition and transmission of these organisms have been markedly advanced by the utilization of the powerful technique of genomic dna fingerprinting. characteristic fingerprints for each bacterial isolate can be produced by cleavage of high molecul ... | 1991 | 1890219 |
| microbiology in the management of destructive periodontal disease. | this paper summarizes the rationale for the application of microbiology in the management of destructive periodontal diseases. the subgingival microbiota in patients with severe periodontitis is complex and contains high numbers of obligate anaerobic bacteria as well as facultative micro-organisms. it has become clear that major differences exist in the composition of the subgingival microflora. these differences are not only quantitative but also qualitative. difference in plaque composition is ... | 1991 | 1890220 |
| dental bacterial plaques. nature and role in periodontal disease. | antony van leeuwenhoek first described oral bacteria. however, not until almost 200 years later was the famous koch postulate introduced. since then, research has extensively been performed regarding the development and microbiology of dental plaques. in spite of the complexity of the developing flora of supragingival plaque, culture studies have shown a remarkably orderly succession of organisms. lately, the concept of microbial specificity in the etiology of periodontal diseases has been widel ... | 1991 | 1890226 |
| effects of immunization with porphyromonas gingivalis and prevotella intermedia on progression of ligature-induced periodontitis in the nonhuman primate macaca fascicularis. | the nonhuman primate (nhp) has proven to be a useful model of human periodontitis. this study describes the immunological characteristics of this model and the ability of active immunization to interfere with ecological changes in the microbiota and its associated disease symptoms. nhps were parenterally immunized with whole-cell antigens of porphyromonas gingivalis and prevotella intermedia. the immunization elicited an approximate 2-log increase in serum immunoglobulin g (igg), igm, and iga is ... | 1991 | 1894349 |
| prostaglandin e release from human monocytes treated with lipopolysaccharides isolated from bacteroides intermedius and salmonella typhimurium: potentiation by gamma interferon. | the purpose of this investigation was to examine gamma interferon potentiation of lipopolysaccharide (lps) responses in human monocytes by using phenol-water-extracted (unfractionated) and highly purified lps preparations isolated from bacteroides intermedius and salmonella typhimurium. phenol-water-extracted lps preparations from these bacteria were further purified by chromatography over sepharose-cl-4b. lps enrichment in pooled column fractions was assessed by sodium dodecyl sulfate-polyacryl ... | 1991 | 1898900 |
| identification, and susceptibility to seven antimicrobial agents, of 61 gram-negative anaerobic rods from periodontal pockets. | sixty-one cultures of gram-negative anaerobic rods were isolated from deep periodontal pockets of patients with rapidly progressive periodontitis. isolates were speciated as bacteroides gingivalis (18 isolates), bacteroides intermedius (8), bacteroides oris (1), bacteroides gracilis (17) and fusobacterium nucleatum (17). their susceptibilities, to seven antimicrobial agents, were determined in vitro using a plate dilution technique. amoxycillin and amoxycillin with clavulanic acid were active ag ... | 1991 | 1901873 |
| microbial aspects of failing osseointegrated dental implants in humans. | the subgingival microflora of 18 failing implants were examined for pathogenic periodontal microorganisms. peptostreptococcus micros was recovered from 6 failing implants, wolinella recta from 6, fusobacterium species from 5, candida albicans from 5, and bacteroides intermedius from 4. enteric rods or pseudomonads constituted a significant part of the microflora in 5 failing implants. actinobacillus actinomycetemcomitans, nonpigmented bacteroides species, capnocytophage species, and staphylococc ... | 1991 | 1906537 |
| isolation and purification of bacteriocin from prevotella intermedia (bacteroides intermedius). | thirty-three out of 46 strains of prevotella intermedia (bacteroides intermedius) isolated from gingival deposits of patients with adult periodontitis possessed the ability to inhibit growth of p. intermedia strain atcc 25611, and 6 of the 33 showed strong inhibitory activity. thirteen isolates did not have the ability to inhibit the growth of p. intermedia strain atcc 25611. an inhibition factor from one of the isolates, p. intermedia th14 which had strong inhibition, was purified and character ... | 1991 | 1920011 |
| immunosuppressive effects of prevotella intermedia on in vitro human lymphocyte activation. | in this study, we have assessed four strains of prevotella intermedia, isolated from periodontally involved lesions, for their ability to inhibit lymphocyte functions. all four strains were found to cause a dose-dependent inhibition of b- and t-cell proliferation in response to mitogens and antigens. this was reflected in altered dna, rna, and protein syntheses. furthermore, p. intermedia appeared to affect the early stages of cell activation. this was ascertained by kinetic analysis in which it ... | 1991 | 1937818 |
| detection and management of the high risk periodontal patient. | periodontal diseases and patients at risk for progression of disease can be diagnosed on the basis of age, previous episodes of periodontal disease and loss of attachment, as detected by periodontal probing and radiographic evaluation. for early onset periodontitis, family history may be important. microbiological evaluations are excellent determinants of disease and also appear to be probable predictors of 'at risk' patients. reliable tests, including chairside tests, are available for the thre ... | 1991 | 1937850 |
| survival of prevotella intermedia (bacteroides intermedius) in transport media. | five transport media were selected for testing, in vitro, the survival of a pure strain of prevotella intermedia (bacteroides intermedius) for 6, 24, and 72 hours. two were non-nutrient transport media (rtf and vmg iv). the three others (tg, py, pyg) were nutrient media. an increase in the transport time, and manipulations in an aerobic atmosphere compromised the survival of the bacteria. py ensured good survival of the bacteria. rtf seems to be the best medium for the transport of p. intermedia ... | 1991 | 1939047 |
| reactivity of igg from explant cultures of periapical lesions with implicated microorganisms. | the presence of igg in periapical inflammatory lesions suggests that immune responses participate in the disease process. the purpose of this investigation was to study the reactivity of igg from the supernatant fluids of explant cultures of periapical lesions with microorganisms implicated in infections of endodontic origin. ninety periapical lesions that had been contiguous with the apex of a root were removed and maintained in explant cultures. a dot-enzyme-linked immunosorbent assay (dot-eli ... | 1991 | 1940739 |
| dna probe detection of periodontal pathogens in hiv-associated periodontal lesions. | recent studies have shown that an atypical gingivitis and a rapidly progressive periodontal disease may be early-occurring opportunistic infections associated with human immunodeficiency virus (hiv) infection. this study examined the prevalence of selected periodontal pathogens associated with these hiv-related periodontal lesions. subgingival plaque samples were obtained from both hiv-seronegative and hiv-seropositive homosexual men and from presumably uninfected heterosexual men. dna probes we ... | 1991 | 1945481 |
| rapid antimicrobial resistance screening method for bacteroides intermedius. | the purpose of the study was to validate a rapid resistance screening (rrs) method for antimicrobial susceptibility testing of a selected periodontopathic microorganism using the standard broth dilution method as a control. twenty-five subgingival plaque samples from gingivitis or periodontitis sites were plated on trypticase soy agar supplemented with 5% rabbit blood with antibiotic discs (rrs method) and without (control). the antibiotics tested were: augmentin, clindamycin, erythromycin, metr ... | 1991 | 1945485 |
| dna probe detection of eikenella corrodens, wolinella recta and fusobacterium nucleatum in subgingival plaque. | this cross-sectional study used species-specific dna probes to examine subgingival plaque specimens for the presence of eikenella corrodens, wolinella recta, and fusobacterium nucleatum in adults with untreated periodontitis or gingivitis and in healthy controls. w. recta and f. nucleatum were more prevalent in diseased sites from the periodontitis group when compared with the controls (81% vs 22% and 83% vs 20% respectively). e. corrodens was detected in 62% of the control sites and 81% of the ... | 1991 | 1945492 |
| effects of propicillin on mixed continuous cultures of periodontal bacteria. | experiments were designed to test the antibiotic (1-phenoxypropyl)penicillin (propicillin) against a complex microflora of periodontal bacteria. this was accomplished by using a continuously growing mixed culture that was obtained by enrichment of periodontal plaque in human serum. peptostreptococcus species, prevotella intermedia, lactobacillus, catenaforme, and streptococcus species were dominant members of the enrichment culture. none of the strains isolated from the enrichment culture exhibi ... | 1991 | 1952836 |
| [effect of chlorhexidine on bacterial fibrinolytic and collagenolytic activity]. | destructive periodontal diseases are characterized by tissue breakdown partly mediated by various enzymes originating from bacteria and/or tissue. collagen and fibronectin are two of the main substrates available for enzyme activity. since chlorhexidine is largely used during and after periodontal therapy, we have investigated the effects of chlorhexidine upon some bacterial proteases. our data indicate that chlorhexidine inhibits, on a dose-dependent manner, both fibronectin and collagen hydrol ... | 1991 | 1960664 |
| [distribution of enzymatically pathogenic bacteria from periodontal pocket in advancing periodontitis]. | production of 9 enzymatic activities of 527 strains freshly isolated from periodontal pockets in advancing periodontitis were investigated. of these isolates, two strains showed lecithinase activity on egg yolk agar plate. collagenase, plasmin and lipase were produced by 28 strains, 26 strains and 22 strains, respectively. two lecithinase-producing strains were identified as bacteroides intermedius. nineteen strains of b. intermedius and 1 strain of fusobacterium species produced lipase on egg y ... | 1990 | 1966847 |
| microbiological study of hiv-related periodontitis. | the subgingival microbiota in 14 persons with hiv-periodontitis was examined. subgingival plaque samples were collected with paper points, transported in vmga iii, and plated on anaerobic enriched brucella blood agar and various selective media. hiv-periodontitis sites revealed actinobacillus actinomycetemcomitans, wolinella recta, peptostreptococcus micros, and bacteroides intermedius, each averaging 7% to 16% of the cultivable subgingival flora in positive patients. high levels of spirochetes ... | 1991 | 2002434 |
| effects of chlorhexidine on proteolytic and glycosidic enzyme activities of dental plaque bacteria. | chlorhexidine was tested for its ability to inhibit a wide range of glycosidic and proteolytic enzyme activities produced by treponema denticola, porphyromonas gingivalis, bacteroides intermedius, actinobacillus actinomycemcomitans, capnocytophaga sputigena, capnocytophaga gingivalis, capnocytophaga orchracea, capnocytophaga sp., actinomyces viscosus, streptococcus mitior, streptococcus mutans, streptococcus sobrinus, streptococcus mitis, streptococcus anginosus, streptococcus oralis and strepto ... | 1991 | 2005230 |
| a randomized, placebo-controlled trial of doxycycline: effect on the microflora of recurrent periodontitis lesions in high risk patients. | twenty-seven patients with a recent history of periodontal abscesses and/or loss of gingival attachment level (gal) despite active periodontal therapy were enrolled in a double-blind, randomized, placebo-controlled trial. clinical measurements and subgingival scaling were performed every 2 months. when a site exhibited greater than or equal to 2 mm loss of gal or a periodontal abscess, patients were administered either doxycycline at a dosage of 200 mg to start and 100 mg per day for 3 weeks, or ... | 1991 | 2027071 |
| immune modulation of prevotella intermedia colonization in squirrel monkeys. | colonization of the gingival crevice by black-pigmented porphyromonas or prevotella spp. (bp/p), including porphyromonas gingivalis (formerly bacteroides gingivalis) and prevotella intermedia (formerly bacteroides intermedius), is thought to be an important ecological event which may result in the destruction of connective tissues supporting the teeth. theoretically, periodontal diseases could be prevented if these or other periodontal pathogenic microorganisms did not colonize the subgingival a ... | 1991 | 2037354 |
| evaluation of acrylic strips containing amoxycillin with clavulanic acid for local drug delivery. | the in vitro release of amoxycillin with clavulanic acid from acrylic strips at initial concentrations of 30, 40 and 50 per cent w/w was monitored using a double-beam ultraviolet spectrophotometer and compared with release of tetracycline hydrochloride. highest levels of the antibacterial agents were released during the first 24 h period. therapeutic levels of the drugs continued to be released during the subsequent 9 day period and were shown to be biologically active. furthermore, for amoxycil ... | 1991 | 2050896 |
| amoxycillin with clavulanic acid and tetracycline in periodontal therapy. | the effects of tetracycline and amoxycillin with clavulanic acid on the clinical parameters and subgingival flora of eight patients with rapidly progressive periodontitis was assessed. subjects received either tetracycline 250 mg four times daily or amoxycillin 250 mg with clavulanic acid 125 mg three times daily for a period of 2 weeks together with subgingival scaling and root planning. both treatment regimens produced significant reductions in bleeding on probing and probing pocket depths whi ... | 1991 | 2050897 |
| development of a rapid qualitative assay for determining elevated antibody levels to periodontopathic organisms. | to allow more widespread use of systemic antibody analysis in clinical settings, a rapid test for determining elevated antibody to periodontitis-associated bacteria was developed. the technique utilizes dot-immunoblotting (dib) on nitrocellulose paper with whole formalinized actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and prevotella intermedia. an elisa was used to compare igg antibody levels to these organisms in venous serum and peripheral capillary blood from 44 subjects. ... | 1991 | 2072241 |
| the formation of hydrogen sulfide and methyl mercaptan by oral bacteria. | the capacity to form volatile sulfur compounds was tested in bacteria isolated from subgingival microbiotas and in a representative number of reference strains. a majority of the 75 tested oral bacterial species and 7 unnamed bacterial taxa formed significant amounts of hydrogen sulfide from l-cysteine. the most active bacteria were found in the genera peptostreptococcus, eubacterium, selenomonas, centipeda, bacteroides and fusobacterium. methyl mercaptan from l-methionine was formed by some mem ... | 1990 | 2082242 |
| characterization and physical mapping of plasmids of black-pigmented bacteroides. | eight species of black-pigmented bacteroides (bpb) were examined for plasmid content. three classes of plasmids with molecular sizes of 16.0 kilobase (kb), 5.4 kb and 5.0 kb were found in 7 of 125 bacteroides intermedius strains. one strain each of porphyromonas (former bacteroides) asaccharolyticus and of bacteroides levii were also shown to have plasmids. however, no plasmids were detected in 90 strains of porphyromonas (bacteroides) gingivalis. the plasmids were purified and digested with res ... | 1990 | 2082244 |
| effect of a triclosan/copolymer/fluoride dentifrice on the oral microflora. | eighty-one human subjects completed a double-blind study which examined the effects of a 0.3% triclosan/2% gantrez copolymer/0.243% sodium fluoride dentifrice on the microflora of supragingival dental plaque. subjects were divided into an experimental group which performed normal oral hygiene with the triclosan/copolymer/fluoride dentifrice and a control group which also performed normal oral hygiene with the same dentifrice minus the triclosan/copolymer. at baseline, 10 weeks, and 28 weeks, sup ... | 1990 | 2083042 |
| an in vitro method to study the adherence of bacteria to saliva-treated tooth enamel sections. | an in vitro bacterial adherence assay which employed human tooth enamel sections precoated with saliva and an epifluorescent staining technique with acridine orange was developed. the assay was used to study the adherence properties of fresh and type strains of the following oral bacterial species: bacteroides gingivalis, bacteroides intermedius, capnocytophaga species, haemophilus aphrophilus, actinobacillus actinomycetemcomitans, peptostreptococcus species, veillonella species, actinomyces isr ... | 1990 | 2087341 |
| the incidence of periodontopathic microorganisms in young children. | thirty-six children in 4 cohorts of 0-1 week, 1-6 months, 1-2 years and 2-2 1/2 years of age were examined for the presence of black-pigmented bacteroides species and some other bacteria suspected of being involved in the subsequent development of periodontal destruction. none of the sought-after bacteria were detected in the first week of life. bacteroides intermedius and bacteroides melaninogenicus were detected as early as 1 month after birth. both of these bacteria were detected in 16-37% of ... | 1990 | 2087345 |
| role of cryptic receptors (cryptitopes) in bacterial adhesion to oral surfaces. | progress in characterizing the receptors that promote bacterial attachment to teeth and oral epithelial cells has suggested that hidden molecular segments may frequently be involved. such cryptic receptors, referred to as 'cryptitopes', may become exposed by several mechanisms. hidden segments of salivary acidic proline-rich proteins evidently become exposed when the molecules undergo a conformational change as they adsorb to apatitic mineral. adhesins of actinomyces viscosus and certain other p ... | 1990 | 2088213 |
| bactericidal effects of human neutrophils and sera on selected endodontic pathogenic bacteria in an anaerobic environment. | phagocytosis by normal human neutrophils and the bactericidal activity of normal human pooled serum were measured under anaerobic conditions on six endodontic pathogenic bacterial species from the genera actinomyces, bacteroides, fusobacterium, peptostreptococcus and streptococcus. the results revealed that actinomyces viscosus was not killed either by human neutrophils or by 20 per cent serum; peptostreptococcus anaerobius was not killed by either 20 per cent or 95 per cent serum; and bacteroid ... | 1990 | 2098335 |
| microbiological features of stable osseointegrated implants used as abutments for overdentures. | the microflora associated with osseointegrated implants used as abutments for overdentures was investigated in 18 edentulous patients, 2 years after implantation. 52.8% of the organisms cultured were facultatively anaerobic cocci and 17.4% were facultatively anaerobic rods, while gram-negative anaerobic rods accounted for only 7.3%. fusobacterium sp. and bacteroides intermedius were both found in 8.8% of the samples. b. gingivalis and spirochetes were not found. repeated microbiological and clin ... | 1990 | 2099207 |