| fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. | fluorescent-dye-conjugated oligonucleotides were used to classify 14 fibrobacter strains by fluorescence microscopy. on the basis of partial 16s rrna sequences of six fibrobacter strains, four hybridization probes were designed to discriminate between the species fibrobacter succinogenes and fibrobacter intestinalis and to identify f. succinogenes subsp. succinogenes. after in situ hybridization to whole cells of the six sequenced strains, epifluorescence microscopy confirmed probe specificity. ... | 1990 | 1688842 |
| taxon-specific probes for the cellulolytic genus fibrobacter reveal abundant and novel equine-associated populations. | a total of six 16s rrna targeted oligonucleotide probes were used to quantify fibrobacter abundance and diversity in the gastrointestinal contents of a pony. approximately 12% of the total 16s rrna extracted from cecal contents hybridized with a fibrobacter genus-specific probe and a fibrobacter succinogenes species-specific probe. however, no significant hybridization was observed with a probe for the species. fibrobacter intestinalis or with three probes for f. succinogenes subspecies. this su ... | 1995 | 7538274 |
| phosphorylation of glucose by a guanosine-5'-triphosphate (gtp)-dependent glucokinase in fibrobacter succinogenes subsp. succinogenes s85. | cell extracts of fibrobacter succinogenes subsp. succinogenes s85 phosphorylated glucose with a gtp-dependent glucokinase. the enzyme showed little activity with atp (12% of that with gtp). of other phosphate donors tested, only dgtp and itp gave high glucokinase activities. dialyzed extracts required mg+2 and k+ for maximal activity. in potassium phosphate buffer, glucokinase showed maximum activity at ph 7.5 with glucose-6-phosphate dehydrogenase as the coupling enzyme. in this assay, glucokin ... | 1994 | 7979872 |
| in vivo 13c nmr study of glucose and cellobiose metabolism by four cellulolytic strains of the genus fibrobacter. | the metabolism of glucose and cellobiose, products of cellulose hydrolysis, was investigated in four cellulolytic strains of the genus fibrobacter: fibrobacter succinogenes s85, 095, hm2 and fibrobacter intestinalis nr9. in vivo 13c nuclear magnetic resonance was used to quantify the relative contribution of glucose and cellobiose to metabolite production, glycogen storage and cellodextrins synthesis in these four strains. the same features were found in all four strains of the genus fibrobacter ... | 1998 | 10335584 |
| characterisation of cellulose-binding proteins that are involved in the adhesion mechanism of fibrobacter intestinalis dr7. | cellulose-binding proteins (cbp) isolated from cell envelopes of the cellulolytic bacterium fibrobacter intestinalis strain dr7 were studied in order to investigate the adhesion mechanism. the proteins were examined for their reaction with antibodies that specifically block bacterial adhesion, response to glycosylation staining and monosaccharide composition. to this end, the effect of some monosaccharides (cbp components) on blocking of dr7 adhesion to cellulose was determined. previous study h ... | 1999 | 10341432 |
| 13c and 1h nuclear magnetic resonance study of glycogen futile cycling in strains of the genus fibrobacter. | we investigated the carbon metabolism of three strains of fibrobacter succinogenes and one strain of fibrobacter intestinalis. the four strains produced the same amounts of the metabolites succinate, acetate, and formate in approximately the same ratio (3.7/1/0.3). the four strains similarly stored glycogen during all growth phases, and the glycogen-to-protein ratio was close to 0.6 during the exponential growth phase. 13c nuclear magnetic resonance (nmr) analysis of [1-13c]glucose utilization b ... | 1998 | 12033219 |
| novel molecular features of the fibrolytic intestinal bacterium fibrobacter intestinalis not shared with fibrobacter succinogenes as determined by suppressive subtractive hybridization. | suppressive subtractive hybridization was conducted to identify unique genes coding for plant cell wall hydrolytic enzymes and other properties of the gastrointestinal bacterium fibrobacter intestinalis dr7 not shared by fibrobacter succinogenes s85. subtractive clones from f. intestinalis were sequenced and assembled to form 712 nonredundant contigs with an average length of 525 bp. of these, 55 sequences were unique to f. intestinalis. the remaining contigs contained 764 genes with blastx simi ... | 2005 | 15901698 |
| features of fibrobacter intestinalis dr7 mutant which is impaired with its ability to adhere to cellulose. | a spontaneous adhesion-defective mutant (dr7-m) of fibrobacter intestinalis dr7 was isolated which was capable of growing on glucose and cellobiose, but impaired in its capacity to degrade cellulose. levels of enzyme activities were determined in solubilized fractions of dr7 and dr7-m. total endoglucanases and xylanase activity values of parent dr7 fractions were 2.84 and 1.85 folds higher than those of the mutant, and were distributed mainly in the bacterial envelope fractions, with some activi ... | 1998 | 16887622 |
| genomic differences between fibrobacter succinogenes s85 and fibrobacter intestinalis dr7, identified by suppression subtractive hybridization. | fibrobacter is a highly cellulolytic genus commonly found in the rumen of ruminant animals and cecum of monogastric animals. in this study, suppression subtractive hybridization was used to identify the genes present in fibrobacter succinogenes s85 but absent from f. intestinalis dr7. a total of 1,082 subtractive clones were picked, plasmids were purified, and inserts were sequenced, and the clones lacking homology to f. intestinalis were confirmed by southern hybridization. by comparison of the ... | 2008 | 18156324 |
| the fibrobacteres: an important phylum of cellulose-degrading bacteria. | the phylum fibrobacteres currently comprises one formal genus, fibrobacter, and two cultured species, fibrobacter succinogenes and fibrobacter intestinalis, that are recognised as major bacterial degraders of lignocellulosic material in the herbivore gut. historically, members of the genus fibrobacter were thought to only occupy mammalian intestinal tracts. however, recent 16s rrna gene-targeted molecular approaches have demonstrated that novel centres of variation within the genus fibrobacter a ... | 2012 | 22213055 |