| rapid presumptive identification of black-pigmented gram-negative anaerobic bacteria by using 4-methylumbelliferone derivatives. | a rapid method for presumptive identification of black-pigmented gram-negative anaerobic rods was developed. using filter paper spot tests for indole production, sialidase, alpha-glucosidase, beta-glucosidase, alpha-fucosidase, and trypsinlike enzyme activities, 100% of porphyromonas gingivalis, prevotella intermedia, and bacteroides levii and 89% of prevotella corporis isolates were correctly identified to the species level. porphyromonas asaccharolytica and porphyromonas endodontalis could not ... | 1991 | 1774320 |
| characterization and physical mapping of plasmids of black-pigmented bacteroides. | eight species of black-pigmented bacteroides (bpb) were examined for plasmid content. three classes of plasmids with molecular sizes of 16.0 kilobase (kb), 5.4 kb and 5.0 kb were found in 7 of 125 bacteroides intermedius strains. one strain each of porphyromonas (former bacteroides) asaccharolyticus and of bacteroides levii were also shown to have plasmids. however, no plasmids were detected in 90 strains of porphyromonas (bacteroides) gingivalis. the plasmids were purified and digested with res ... | 1990 | 2082244 |
| comparative inhibition of bacterial and microsomal 3-ketodihydrosphingosine synthetases by l-cycloserine and other inhibitors. | eleven compounds were examined for their capacity to inhibit the first enzyme of the sphingolipid pathway, 3-ketodihydrosphingosine synthetase. of these, l-cycloserine was the most potent, affecting both bacterial and brain microsomal enzymes to a significant degree at 0.04 mm. d- and l-cycloserine irreversibly inactivated the enzyme, indicating a suicide substrate mode of action. l-cycloserine was a more potent inhibitor of the growth of bacteroides levii than was d-cycloserine, indicating that ... | 1984 | 6486763 |
| l-cycloserine inhibition of sphingolipid synthesis in the anaerobic bacterium bacteroides levii. | l-cycloserine was found to significantly inhibit the activity of the first enzyme of the sphingolipid pathway when added to growing cultures of bacteroides levii. the effect of cycloserine on the synthesis of the sphingolipids showed that ceramidephosphorylethanolamine was inhibited to a greater degree than ceramidephosphorylglycerol, although synthesis of both was significantly inhibited by cycloserine as determined by [32p] incorporation and phosphorus determination. in contrast, synthesis of ... | 1984 | 6712658 |
| update on the taxonomy and the clinical and laboratory characteristics of pigmented anaerobic gram-negative rods. | pigmented anaerobic gram-negative rods are currently categorized as 17 species distributed in three genera: prevotella, porphyromonas, and bacteroides. these organisms are often encountered in clinical specimens but are also found as part of the indigenous flora on various mucosal surfaces. several studies are presently assessing the association of individual species with health and disease. for example, porphyromonas gingivalis and porphyromonas endodontalis are key putative pathogens in adult ... | 1995 | 7548548 |
| bacteroides levii-like organisms isolated from clinical specimens. | | 1995 | 7548554 |
| intrauterine bacterial findings in postpartum cows with retained fetal membranes. | eleven swedish postpartum cows with retained fetal membranes (rfm) were studied to determine the intrauterine bacterial flora. bacteriological examination was performed from twice weekly uterine biopsies. a total of 161 biopsies were collected during the first 8 weeks postpartum of which 82 (50.9%) were found with bacterial growth. seventy-one of the 82 bacteria-positive biopsies (86.6%) showed mixed infections whereas the remaining 11 (13.4%) were pure cultures. generally, a total of 322 isolat ... | 1994 | 7732744 |
| phylogeny of bacteroides, prevotella, and porphyromonas spp. and related bacteria. | the phylogenetic structure of the bacteroides subgroup of the cytophaga-flavobacter-bacteroides (cfb) phylum was examined by 16s rrna sequence comparative analysis. approximately 95% of the 16s rrna sequence was determined for 36 representative strains of species of prevotella, bacteroides, and porphyromonas and related species by a modified sanger sequencing method. a phylogenetic tree was constructed from a corrected distance matrix by the neighbor-joining method, and the reliability of tree b ... | 1994 | 8300528 |
| the normal vaginal flora, h2o2-producing lactobacilli, and bacterial vaginosis in pregnant women. | in this study of the vaginal flora of 171 pregnant women in labor at term, the flora was categorized as normal (lactobacillus predominant), intermediate, or representative of bacterial vaginosis (bv) on the basis of a vaginal smear. bv was diagnosed in 39 women (23%); the vaginal flora was classified as normal in 50% of cases and as intermediate in 27%. h2o2-producing lactobacilli were recovered from 5% of women with bv, 37% of those with an intermediate flora, and 61% of those with a normal flo ... | 1993 | 8324131 |
| porphyromonas gingivalis, actinobacillus actinomycetemcomitans and treponema denticola detection in oral plaque samples using the polymerase chain reaction. | detection of putative pathogens is critical for delineating the etiology and progression of periodontitis. in the present study, we have used a polymerase chain reaction (pcr) assay utilizing primers specific for the lkt a gene of actinobacillus actinomycetemcomitans, the fimbrial gene of porphyromonas gingivalis, and tdp a gene of treponema denticola in order to determine the presence of these pathogens in subgingival plaque samples from periodontitis sites. these gene specific primers were als ... | 1996 | 8707980 |
| spirochaetes and other bacterial species associated with bovine digital dermatitis. | the 16s rrna genes from spirochaetes associated with digital dermatitis of british cattle were amplified by polymerase chain reaction from digital dermatitis lesion biopsies using one universal and one treponeme-specific primer. two treponemal sequences were identified both of which shared a high degree of homology with the oral pathogen treponema denticola (98%). two further 16s rrna gene sequences were obtained and shared similarity to bacteroides levii (99%) and mycoplasma hyopharyngis (98%). ... | 1997 | 9368358 |
| comparison of ceftiofur sodium and oxytetracycline for treatment of acute interdigital phlegmon (foot rot) in feedlot cattle. | to determine whether ceftiofur sodium would be useful for treatment of acute interdigital phlegmon (foot rot) in cattle. | 1998 | 9448831 |
| bovine polymorphonuclear neutrophil-mediated phagocytosis and an immunoglobulin g2 protease produced by porphyromonas levii. | acute interdigital phlegmon (aip) is a commonly occurring anaerobic bacterial infection in cattle. this study examined in vitro the interaction of bovine polymorphonuclear granulocytic neutrophils (pmn) from blood with bacterial species involved in aip. polymorphonuclear neutrophils were purified from whole bovine blood, exposed to one of the three putative etiologic agents of aip and comparatively assessed for phagocytosis using light microscopy. fusobacterium necrophorum and prevotella interme ... | 1999 | 10369568 |
| detection of tetq and ermf antibiotic resistance genes in prevotella and porphyromonas isolates from clinical specimens and resident microbiota of humans. | gram-negative anaerobes belonging to the genera fusobacterium, prevotella and porphyromonas were investigated for the presence of tetq and ermf, which have been shown to be spread by conjugal elements. one hundred isolates from either sites of infection or various body sites in healthy subjects were studied. pcr was used to detect tetq, and dna-dna hybridization studies on ecori chromosomal digests were undertaken to detect the presence of tetq and ermf. antibiotic sensitivity assays were perfor ... | 2000 | 10797077 |
| in vitro expression of tumor necrosis factor-alpha, interleukin 1beta, and interleukin 8 mrna by bovine macrophages following exposure to porphyromonas levii. | the objective was to evaluate the pro-inflammatory response of bovine macrophages towards porphyromonas levii, an etiologic agent of acute interdigital phlegmon, by evaluating the mrna expression of tumor necrosis factor-alpha (tnf-alpha), interleukin 1beta (il-1beta), and interleukin 8 (il-8). bovine macrophages detect the presence of bacteria, such as p. levii, and respond by upregulating transcription of the genes for pro-inflammatory cytokines tnf-alpha and il-1beta in addition to the neutro ... | 2002 | 11989740 |
| chemotaxis, phagocytosis, and oxidative metabolism in bovine macrophages exposed to a novel interdigital phlegmon (foot rot) lesion isolate, porphyromonas levii. | to examine the host response toward porphyromonas levii, by evaluating chemotaxis, phagocytosis, and oxidative burst of bovine macrophages in vitro. | 2002 | 12013480 |
| bovine necrotic vulvovaginitis associated with porphyromonas levii. | an outbreak of bovine necrotic vulvovaginitis associated with porphyromonas levii, an emerging animal and human pathogen, affected 32 cows on a dairy farm in the northeast of israel. five animals had to be culled. this report appears to be the first that associates p. levii with bovine necrotic vulvovagnitis. | 2004 | 15109423 |
| porphyromonas somerae sp. nov., a pathogen isolated from humans and distinct from porphyromonas levii. | porphyromonas levii is an anaerobic, pigmented gram-negative bacillus originally isolated from bovine rumen. we describe 58 human clinical strains of p. levii-like organisms, isolated from various human clinical specimens that are phenotypically similar to the type strain of p. levii, a rumen isolate (atcc 29147). our biochemical, comparative 16s rrna sequence analyses, and dnalpha-dna relatedness studies indicate that the human p. levii-like organisms are similar to each other but genetically d ... | 2005 | 16145091 |
| evaluation of the effects of short-chain fatty acids and extracellular ph on bovine neutrophil function in vitro. | to investigate the effects of short-chain fatty acids (scfas) and ph on neutrophil oxidative burst, phagocytosis, and morphology after exposure to acetate, propionate, butyrate, or succinate at ph 5.5 and 6.7. | 2006 | 17078753 |
| effects of bovine necrotic vulvovaginitis on productivity in a dairy herd in israel. | bovine necrotic vulvovaginitis (bnvv) is characterized by the development of a necrotic vulvovaginal lesion, almost exclusively in post-parturient first-lactation cows, associated with porphyromonas levii. the scope of this survey was to evaluate the impact of bnvv on herd productivity as a means to rationally evaluate the resources that should be allocated in dealing with the syndrome. during an outbreak of bnvv in a dairy herd, following the introduction of a large number of cows from another ... | 2008 | 17433735 |
| sample-based assessment of the microbial etiology of bovine necrotic vulvovaginitis. | a semiquantitative evaluation of potential bacterial pathogens was correlated to the severity of lesions during an outbreak of bovine necrotic vulvovaginitis (bnvv) on an israeli dairy herd. bacteriologic examination of 287 vaginal swabs from 104 post-calving heifers showed a highly significant correlation between porphyromonas levii colony forming unit numbers and the clinical scores of the lesions, when assessed by an ordinal regression statistical model. no such correlation was found for the ... | 2007 | 17553557 |
| isolation of porphyromonas levii from vaginal samples from cows in herds negative for bovine necrotic vulvovaginitis. | | 2008 | 19103617 |
| identification of porphyromonas levii isolated from clinical cases of bovine interdigital necrobacillosis by 16s rrna sequencing. | laboratories use pigmentation, antibiotic susceptibility, and biochemical tests to identify anaerobic organisms that play a role in bovine interdigital necrobacillosis (bovine foot rot). in this study, 16s rrna gene sequencing was used to identify strains to the species level that were originally classified as prevotella or porphyromonas spp by conventional phenotype assessment methods. of 264 qualified strains from ceftiofur clinical trials, 241 isolates were definitively identified by 16s rrna ... | 2009 | 20425726 |
| detection of antibodies against fusobacterium necrophorum and porphyromonas levii-like species in dairy cattle with papillomatous digital dermatitis. | bovine digital epidermitis involves different pathologies, including pdd, interdigital dermatitis, and foot rot. bacteriological and molecular biological studies suggest that these are multimicrobial infections. during our study on the isolation of treponemes from biopsies of pdd, colonies producing black pigment were isolated frequently from the primary cultures, suggesting that porphyromonas species were present. moreover, 16s rrna genes of fusobacterium necrophorum and porphyromonas levii-lik ... | 2010 | 20536732 |
| the effects of bovine necrotic vulvo-vaginitis on reproductive and production performance of israeli 1st calf heifers. | bovine necrotic vulvovaginitis (bnvv) is a syndrome unique to israel characterized by necrotic lesion in the caudal vagina mainly in first calf heifers after calving, associated with porphyromonas levii. the objectives of this study were to analyze the impact of bnvv on reproductive performance, milk production and survival in the heard of first calf dairy heifers in affected farms, and to verify if the effects of bnvv are severity-dependent. for assessment of the severity level a scale of 4 deg ... | 2012 | 22225696 |
| pyogranulomatous pneumonia in goats caused by an undescribed porphyromonas species, "porphyromonas katsikii". | a yet-undescribed bacterial species, tentatively named "porphyromonas katsikii," was isolated from individuals of a small goat herd with pyogranulomatous pneumonia during an outbreak of acute respiratory disease. the isolated bacteria grew in the form of black-pigmented colonies after 14 days of incubation under anaerobic conditions at 37°c on a tryptic soy blood agar medium. the bacteria were identified as a yet-undescribed porphyromonas species by determination of the nucleotide sequence of th ... | 2014 | 25540395 |
| presence and localization of bacteria in the bovine endometrium postpartum using fluorescence in situ hybridization. | the aim of this study was to investigate bacterial invasiveness of the bovine endometrium during the postpartum period. fluorescence in situ hybridization was applied to endometrial biopsies using probes for fusobacterium necrophorum, porphyromonas levii, trueperella pyogenes, escherichia coli and a probe for bacteria in general (the overall domain bacteria) to determine their tissue localization. holstein cows were sampled at three time points postpartum (t1: 4-12 days postpartum, t2: 24-32 day ... | 2017 | 28237333 |
| bacterial invasion of the uterus and oviducts in bovine pyometra. | pyometra is a common disease of cattle that causes infertility and thereby financial losses to the cattle industry. bacteria involved in the development and progression of pyometra have been investigated by microbial culture but their tissue invading abilities, which is an important aspect of bacterial pathogenicity and development of lesions, have not been investigated. bacterial invasion of the uterus and oviducts was studied in 21 cows diagnosed with pyometra at the time of slaughter by apply ... | 2017 | 28257873 |
| potential bacterial core species associated with digital dermatitis in cattle herds identified by molecular profiling of interdigital skin samples. | although treponemes are consistently identified in tissue from bovine digital dermatitis (dd) lesions, the definitive etiology of this debilitating polymicrobial disease is still unresolved. to study the microbiomes of 27 dd-infected and 10 healthy interdigital skin samples, we used a combination of different molecular methods. deep sequencing of the 16s rrna gene variable regions v1-v2 showed that treponema, mycoplasma, fusobacterium and porphyromonas were the genera best differentiating the dd ... | 2016 | 27016768 |