| comparison of the benzoyl-dl-arginine-naphthylamide (bana) test, dna probes, and immunological reagents for ability to detect anaerobic periodontal infections due to porphyromonas gingivalis, treponema denticola, and bacteroides forsythus. | most forms of periodontal disease are associated with the presence or overgrowth of anaerobic species that could include treponema denticola, porphyromonas gingivalis, and bacteroides forsythus among others. these three organisms are among the few cultivable plaque species that can hydrolyze the synthetic trypsin substrate benzoyl-dl-arginine-naphthylamide (bana). in turn, bana hydrolysis by the plaque can be associated with periodontal morbidity and with the presence of these three bana-positiv ... | 1992 | 1311335 |
| a sensitive enzymatic method (sk-013) for detection and quantification of specific periodontopathogens. | porphyromonas gingivalis, bacteroides forsythus, and treponema denticola have been found to predominate in periodontal pockets of patients with adult periodontitis. these microorganisms hydrolyze the synthetic peptide n-benzoyl-dl-arginine-2-naphthylamide (bana). in this study, we developed an enzymatic method, designated sk-013, to detect the existence of these microorganisms in subgingival plaque bacteria. this enzymatic method was based on the observation of the hydrolysis of n-carbobenzoxy-g ... | 1992 | 1312591 |
| a sensitive enzymatic method (sk-013) for detection of treponema denticola, porphyromonas gingivals and bacteroides forsythus in subgingival plaque samples. | an enzymatic method, sk-013, was developed for rapid detection of the peptidase activity in subgingival plaque samples. this method was found to have specificity for porphyromonas gingivalis, treponema denticola, bacteroides forsythus, and some capnocytophaga strains. the purpose of this study was to determine whether sk-013 could indicate the presence of periodontopathic bacteria, including t. denticola, p. gingivalis and b. forsythus, which produce trypsin-like enzymes. subgingival plaque samp ... | 1992 | 1312592 |
| microbial etiology of periodontal diseases. where are we? where are we going? | many studies indicate a strong association of actinobacillus actinomycetemcomitans with localized juvenile periodontitis. species associated with adult periodontitis include bacteroides forsythus, porphyromonas gingivalis, prevotella intermedia, a. actinomycetemcomitans, and wolinella recta. capnocytophaga species may be important in pubertal gingivitis. an unnamed spirochete related to treponema pallidum has been identified in acute necrotizing ulcerative gingivitis lesions. species isolated fr ... | 1992 | 1325846 |
| growth of porphyromonas gingivalis, treponema denticola, t. pectinovorum, t. socranskii, and t. vincentii in a chemically defined medium. | a chemically defined medium, omiz (oral microbiology and immunology, zürich)-w1 was developed. medium omiz-w1 supports the long-term proliferation of a wide range of oral anaerobes, including representative strains of four treponema species and porphyromonas gingivalis. high concentrations of ascorbic acid and ammonium ions proved to be important for the growth of these organisms. t. denticola cd-1 grew in the absence of polyamines and long-chain fatty acids, t. pectinovorum and t. socranskii re ... | 1992 | 1400984 |
| periodontal pathogens in the shallow pockets of immigrants from developing countries. | the aim of this study was to examine the distribution of typical periodontitis-associated microorganisms in refugees arriving from non-industrialized countries, and to relate the presence of these organisms to the periodontal condition of the subjects. thirty males between 35-44 years of age were surveyed. dental plaque, calculus, gingivitis, loss of attachment, and probing depths were recorded for all surfaces. a total of 90 microbiological samples were taken with paper points from mesial sites ... | 1992 | 1494449 |
| subgingival distribution of periodontal pathogenic microorganisms in adult periodontitis. | the association between specific plaque microorganisms and periodontal diseases has been the subject of much recent interest due to its potential importance in the diagnosis and classification of these diseases. in order to optimize microbiological tests in periodontal therapy, it is important to know how many subgingival plaque samples must be assayed from a single patient in order to ascertain infection with a periodontal pathogen. to answer this question the present study assessed the distrib ... | 1992 | 1527685 |
| comparison of various detection methods for periodontopathic bacteria: can culture be considered the primary reference standard? | the development of diagnostic tests for a periodontal infection raises the issue as to what the appropriate reference standard, or "gold standard," should be for the evaluation of a new test. the present research was initiated to compare the ability of several detection methods, i.e., a serial dilution anaerobic culture and/or microscopic procedure, a dna probe procedure, and immunological reagents using both an enzyme-linked immunosorbent assay and an indirect immunofluorescence assay to detect ... | 1992 | 1537912 |
| rapid presumptive identification and further characterization of bacteroides forsythus. | bacteroides forsythus is a fastidious anaerobic gram-negative organism associated with various forms of periodontal disease. it is dependent on n-acetylmuramic acid for growth. a method for rapid presumptive identification of human-derived strains of b. forsythus is presented, based on the following eight criteria: (i) positive activity for alpha-glucosidase, (ii) positive activity for beta-glucosidase, (iii) positive activity for sialidase, (iv) positive activity for trypsinlike enzyme, (v) neg ... | 1992 | 1551981 |
| [polymorphonuclear leukocyte-mediated effects on human oral keratinocytes by periodontopathic bacterial extracts]. | in order to investigate the polymorphonuclear leukocyte-mediated oral keratinocyte injury, the author established a method to culture keratinocytes (scc9 and gingival keratinocytes) and subjected to the cell detachment assay by polymorphonuclear leukocytes and/or bacterial ultrasonic extracts from actinobacillus actinomycetemcomitans y4, bacteroides forsythus omz 408, fusobacterium nucleatum atcc 10953, capnocytophaga sputigena 4 and eikenella corrodens 1073. the bacterial extracts alone caused ... | 1992 | 1607829 |
| analysis of site specific periodontal bacteria sampling schemes. | a great deal of controversy has existed in the periodontal literature as to whether the site or the subject should be the unit of analysis. using the site as the unit of analysis assumes that observations of sites within the same subject are independent and ignores between subject variation. the purpose of this report is to evaluate the influence that the unit of analysis has on estimating the number of necessary site specific bacterial samples from each subject. the number of bacterial samples ... | 1992 | 1625150 |
| use of synthetic oligonucleotide dna probes for identification and direct detection of bacteroides forsythus in plaque samples. | oligonucleotide dna probes complementary to the hypervariable region of the 16s rrna of bacteroides forsythus were tested for their specificity and sensitivity against reference and clinical isolates of 73 different species of bacteria. the probes were used to detect the organism directly from nucleic acid extracts of subgingival plaque samples, and the results were compared with results of detection by culture methods. the data demonstrate that the probes are very specific (100%) and sensitive ... | 1991 | 1719022 |
| [first results with a microbiological quick diagnostic procedure in periodontitis]. | we tested a new diagnostic procedure for the characterization of subgingival plaque in 119 subjects suffering from adult periodontitis. the method can be characterized as a hydrolysis of bana (n-benzoyl-dl-arginine-beta-naphthylamide) by peptidases of porphyromonas gingivalis, bacteroides forsythus and treponema denticola. the result of a positive will be a more or less blue coloured test paper representing the amount of these three peridontal pathogens into the subgingival plaque. after the per ... | 1991 | 1818626 |
| cellular fatty acids of bacteroides forsythus. | | 1990 | 2081760 |
| effect of a triclosan/copolymer/fluoride dentifrice on the oral microflora. | eighty-one human subjects completed a double-blind study which examined the effects of a 0.3% triclosan/2% gantrez copolymer/0.243% sodium fluoride dentifrice on the microflora of supragingival dental plaque. subjects were divided into an experimental group which performed normal oral hygiene with the triclosan/copolymer/fluoride dentifrice and a control group which also performed normal oral hygiene with the same dentifrice minus the triclosan/copolymer. at baseline, 10 weeks, and 28 weeks, sup ... | 1990 | 2083042 |
| monoclonal antibodies for the detection of 'periodontopathic' bacteria. | the development of indirect immunofluorescence assays using mabs as specific probes has made it possible to detect a variety of suspected periodontal pathogens in subgingival plaque, with exquisite sensitivity. the studies demonstrate high reproducibility of the results if different mabs are used to assess the same bacterial species. bacteroides forsythus, bact. gingivalis, bact. intermedius and wolinella recta, but not actinobacillus actinomycetemcomitans were found in sites of adult periodonti ... | 1990 | 2088218 |
| [a rapid diagnosis (sk-013) for periodontitis based on the enzymatic activity of periodontopathic bacteria. study on the characterization of the sk-013 and the enzyme specificity]. | we present studies for development of an enzymatic diagnostic method for periodontitis. a rapid and sensitive diagnostic method named sk-013 was provided by sunstar inc. to evaluate the peptidase activities specifically derived from periodontopathic bacteria such as bacteroides gingivalis, bacteroides forsythus and treponema denticola and some strains of capnocytophaga species. the results obtained indicated the specificity of the enzymatic reaction in this system. | 1990 | 2133684 |
| [a rapid diagnosis (sk-013) for periodontitis based on the enzymatic activity of periodontopathic bacteria. the relationship between the enzymatic activity (sk-013) and b. gingivalis, b. forsythus, t. denticola in subgingival microflora]. | sk-013 was developed for rapid detection of the peptidase activity in subgingival plaque samples. the purpose of this study was to determine whether sk-013 could be a marker for the presence of periodontopathic bacteria including treponema denticola, bacteroides gingivalis and bacteroides forsythus which produce trypsin-like enzyme. subgingival plaque samples were taken with 3 paper points from 10 clinically healthy sites and 30 periodontal diseased sites. the sk-013 activity of plaque sample wa ... | 1990 | 2133685 |
| [a rapid diagnosis for periodontitis based on the enzymatic activity of periodontopathic bacteria. the application of the sk-013 to the judgement on the effect of initial preparation]. | the purpose of the present study was to examine the diagnostic value of the sk-013 (sunstar, osaka) in evaluating the effect of initial preparation. the sk-013 is a highly sensitive reagent to measure peptidase activity specifically produced by bacteroides gingivalis. bacteroides forsythus, and treponema denticola. thirty-six sites in 16 periodontitis patients were monitored in this study. the clinical status of each site was assessed using the clinical indices such as gingival index (gi), plaqu ... | 1990 | 2133687 |
| multi-center clinical evaluation of a chairside method for detecting certain periodontopathic bacteria in periodontal disease. | the association of bacteroides gingivalis, bacteroides forsythus, treponema denticola, and actinobacillus actinomycetemcomitans among others with periodontal disease offers the opportunity for the development of diagnostic tests that are based upon the detection and/or quantification of one or more of these organisms or their by-products in the plaque. three of the putative periodontal pathogens namely, t. denticola, b. gingivalis, and b. forsythus, can hydrolyze the synthetic trypsin substrate, ... | 1990 | 2181111 |
| the utility of the bana test for monitoring anaerobic infections due to spirochetes (treponema denticola) in periodontal disease. | treponema denticola, porphyromonas gingivalis, and bacteroides forsythus each possesses an enzyme(s) that hydrolyzes the synthetic substrate benzoyl-dl-arginine-naphthylamide (bana). the presence of these organisms in a subgingival plaque sample can be determined by the ability of the plaque to hydrolyze bana. in the present study, we describe the usefulness of the bana test at various stages of a clinical trial of the efficacy of metronidazole in the treatment of periodontal disease. a bana-pos ... | 1990 | 2212216 |
| development of a diagnostic test for anaerobic periodontal infections based on plaque hydrolysis of benzoyl-dl-arginine-naphthylamide. | treponema denticola, porphyromonas (bacteroides) gingivalis, and bacteroides forsythus are among the anaerobic species frequently associated with adult forms of periodontal disease. these organisms hydrolyze the synthetic peptide benzoyl-dl-arginine-naphthylamide (bana), and such enzyme activity can be detected in the plaque and related to clinical disease and the presence of spirochetes. in this investigation, the liquid bana assay was compared with a commercially developed bana assay which emp ... | 1990 | 2380379 |
| prevalence of bacteroides forsythus and bacteroides gingivalis in subgingival plaque of prosthodontically treated patients on short recall. | the prevalence of bacteroides forsythus and bacteroides gingivalis in subgingival plaque of patients on short recall was analyzed in relation to the probing depth of the test sites. the subjects had excellent oral hygiene and therefore were unlikely to suffer from active periodontal destruction. sixty-four subgingival plaque samples, taken from gingival or periodontal pockets with probing depths ranging from 1 to 8 mm, were quantitatively assessed for the presence of the two species using specie ... | 1989 | 2524576 |
| effects of periodontopathic bacteria on il-1 and il-1 inhibitor production by human polymorphonuclear neutrophils. | the effect of heat-killed periodontopathic bacteria on the production of interleukin-1 (il-1) and an il-1 inhibitor by human polymorphonuclear neutrophils (pmn's) was examined. peripheral blood was obtained from 18 healthy volunteers and the pmn's were separated using dextran sedimentation and ficoll-paque density gradient centrifugation. the pmn's (5 x 10(5) cells/well) were cultured in serum-free media with or without heat-killed periodontopathic bacteria. four gram-negative periodontopathic b ... | 1989 | 2640313 |
| clinical, microbiological and immunological studies of post-juvenile periodontitis. | the present study includes the clinical, microbiological and immunological examinations of 2 patients with post-juvenile periodontitis. bacteroides intermedius was the predominant isolate from periodontal pockets with post-localized juvenile periodontitis. bacteroides gingivalis, bacteroides forsythus and actinobacillus actinomycetemcomitans were detected in samples from periodontal pockets with post-generalized juvenile periodontitis. igg antibody levels to b. gingivalis were significantly high ... | 1989 | 2640920 |
| newly delineated periodontal pathogens with special reference to selenomonas species. | many new species have been isolated from subgingival periodontal pockets, for example wolinella recta and bacteroides forsythus, reflecting better basic microbiological techniques. other species were created from existing species as a result of better characterization methods, i.e. bacteroides buccae and bacteroides oris. we can recognize different types of periodontal disease and can find differences relating to the progressive compared to inactive lesions. data illustrated in this presentation ... | 1989 | 2661440 |
| [microbial flora of periodontitis and monitoring of the effect of initial preparation by immunofluorescence microscopy]. | the effect of initial preparation in adult periodontitis was evaluated by changes in clinical parameters and immunofluorescence microscopic counts of periodontal disease associated bacteria. subgingival plaque samples were taken with sterilized paper points from 10 sites of 5 periodontally healthy persons and 44 sites of 23 adult periodontitis patients. twenty-one diseased sites were periodically examined after plaque control and scaling, root planing. the direct immunofluorescence technique was ... | 1989 | 2700361 |
| dependence of proliferation of bacteroides forsythus on exogenous n-acetylmuramic acid. | bacteroides forsythus is the first wild-type or mutant bacterium found to require exogenous n-acetylmuramic acid for proliferation and maintenance of cell shape. this implies so far unknown pathways for peptidoglycan synthesis and a strict dependence of b. forsythus on other bacteria in its oral habitat. addition of n-acetylmuramic acid to conventional bacteriological media allows routine cultivation of this fastidious organism. | 1989 | 2722237 |
| the microbiota of early periodontitis lesions in adults. | the predominant cultivable microbiota of shallow progressive (active) periodontal lesions in 4 adults (29-40 years) was compared to that of shallow inactive sites. periodontal attachment levels were monitored longitudinally, and sites showing 2-3 mm attachment loss in the previous 1-2 months were considered disease active. inactive sites showed no change in attachment level. the microbiota of shallow sites was dominated by gram negative rod species including wolinella recta, bacteroides forsythu ... | 1989 | 2768541 |
| production and characterization of monoclonal antibodies against bacteroides forsythus and wolinella recta. | hybrid cell lines producing monoclonal antibodies against bacteroides forsythus or wolinella recta were generated by fusion of sp2/0 or fo myeloma cells with splenocytes from balb/c mice immunized with formalinized cells of b. forsythus fdc 331 or w. recta d13a-g, respectively. enzyme-linked immunosorbent assays and indirect immunofluorescence tests were used to analyze the distribution of the recognized antigens on a panel of 70 strains representing 35 taxa, most of which are members of the ora ... | 1988 | 3170894 |
| ultrastructure of the cell envelope of bacteroides forsythus strain atcc 43037t. | | 1988 | 3269995 |
| bacteroides forsythus in adult gingivitis and periodontitis. | | 1987 | 3333825 |
| development of a broth medium for bacteroides forsythus. | bacteriodes forsythus has been implicated as a possible pathogen in certain destructive periodontal diseases. this species has been extremely difficult to grow in broth, thus hampering its characterization and identification. a broth medium was developed consisting of 30 g of phytone, 10 g of nacl, and 1 g of filter-sterilized l-cysteine per liter. the medium supported the growth of 23 fresh isolates of b. forsythus from six subjects to optical densities averaging 0.71 +/- 0.20 (standard deviati ... | 1987 | 3584427 |
| the relationships between streptococcal species and periodontopathic bacteria in human dental plaque. | the existence of antagonistic and commensal relationships between microorganisms was investigated. the predominant cultivable flora in 172 plaque samples from active and non-active sites in 32 human subjects with destructive periodontitis was determined. the presence of putative periodontopathic organisms (bacteroides gingivalis, bacteroides intermedius, bacteroides forsythus, wolinella recta, actinobacillus actinomycetemcomitans, and eikenella corrodens) in a site was correlated with the absenc ... | 1985 | 3868968 |
| api zym and api an-ident reactions of fastidious oral gram-negative species. | api zym and api an-ident enzymatic substrate tests were done on six oral species which are difficult to characterize with conventional biochemical tests. "bacteroides forsythus, the "fusiform" bacteroides species (a. c. r. tanner, m. a. listgarten, m. n. strzempko, and j. l. ebersole, manuscript in preparation), is difficult to cultivate in broth media, yet it gave 15 positive tests in these series. the tests were able to separate this new species from species of capnocytophaga and fusobacterium ... | 1985 | 3930558 |
| support of peptide-dependent growth of bacteroides forsythus by synthetic fragments of haemoglobin or fetuin. | the putative periodontal pathogen bacteroides forsythus is a fastidious gram-negative anaerobe with high proteolytic activity. for growth in a chemically defined medium containing insulin it required serum. serum could be replaced by human haemoglobin or bovine asialofetuin, or by proteolytic fragments of these two proteins. four such fragments consisting of from 8 to 18 amino acid residues were isolated and sequenced. only aspartic acid, threonine, and valine were common to all peptides. an und ... | 1993 | 7507661 |
| detection of putative periodontal pathogens in subgingival specimens by 16s ribosomal dna amplification with the polymerase chain reaction. | the utility of the 16s ribosomal rna-based polymerase chain reaction (pcr) for detection of actinobacillus actinomycetemcomitans, bacteroides forsythus, campylobacter rectus, eikenella corrodens, porphyromonas gingivalis, prevotella intermedia, and treponema denticola was examined and compared with that of anaerobic culture. primer pairs consisting of 20-27 nucleotides amplified 404- to 688-bp regions of 16s ribosomal rna genes of these organisms. this method had a lower detection limit of 50 ta ... | 1995 | 7548580 |
| patterns of antibody response in subjects with periodontitis. | periodontal diseases comprise a heterogeneous group of infections that are difficult to distinguish on a clinical basis alone. the purpose of the present investigation was to group periodontitis subjects according to their elevated serum antibody levels to specific subgingival species. a total of 119 subjects (19-70 years) with evidence of prior periodontal destruction were monitored at 2-month intervals (maximum 8 visits), prior to therapy, using clinical parameters measured at 6 sites per toot ... | 1995 | 7567061 |
| identification of bacteroides forsythus in subgingival plaque from patients with advanced periodontitis. | bacteroides forsythus has been associated with destructive adult periodontitis. up to now, detailed analysis by classical means was hampered by the fastidious nature of the organism. there is hope that the application of molecular detection methods such as indirect immunofluorescence or in situ hybridization (ish) will allow for more rapid and accurate identification. here we describe a b. forsythus-specific probe (bfv530), complementary to 16s rrna, which correctly identified all b. forsythus i ... | 1993 | 7681850 |
| fluorescence in situ hybridization for direct visualization of gram-negative anaerobes in subgingival plaque samples. | fluorescent oligonucleotide probes complementary to variable regions of porphyromonas gingivalis and bacteroides forsythus 16s ribosomal rna were used to identify these organisms in smears of formaldehyde-fixed subgingival plaque samples from patients suffering from periodontitis. fluorescence in situ hybridization represents a useful method for assessing the microbial ecology of the periodontal flora. | 1993 | 7686071 |
| predominant microflora of severe, moderate and minimal periodontal lesions in young adults with rapidly progressive periodontitis. | the purpose of this investigation was to study the microflora of severe, moderate and minimal periodontal lesions, in young adults with rapidly progressive periodontitis (rpp). subgingival plaque samples were taken from 142 periodontal lesions in 10 young adults aging 25 to 35 years. the examination of the subgingival microflora indicated that certain species, including porphyromonas gingivalis, bacteroides forsythus, fusobacterium nucleatum, actinobacillus actinomycetemcomitans, and campylobact ... | 1995 | 7722848 |
| detection of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and bacteroides forsythus in an a. actinomycetemcomitans-positive patient population. | a population of 33 subjects were selected on the basis that all had tested positive for a, actinomycetemcomitans at some time during the prior 7 years. most subjects (31/33) belonged to families with a proband with confirmed localized juvenile periodontitis (jp); however, most subjects had no evidence of the typical lesions associated with jp. two additional subjects with rapidly progressive periodontitis, known to be positive for a. actinomycetemcomitans, were also recruited. the patients with ... | 1995 | 7730968 |
| comparative detection frequency of 6 putative periodontal pathogens in sudanese and norwegian adult periodontitis patients. | twenty-five sudanese and 18 norwegian adult periodontitis patients were selected to participate in this study. the purpose was to compare cultivation results of porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetemcomitans, fusobacterium nucleatum, and capnocytophaga species as well as various enteric rods in both populations. in addition, dna probe analysis was used to identify p. gingivalis, p. intermedia, a. actinomycetemcomitans, f. nucleatum, and bacteroides forsythu ... | 1994 | 7853128 |
| microbial associations of 4 putative periodontal pathogens in sudanese adult periodontitis patients determined by dna probe analysis. | the few previous cultivation studies on the in vivo associations between various periodontal microbial species have shown several positive and negative associations. the present investigation utilized dna probe analysis to examine possible in vivo associations between the periodontal pathogens porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, and bacteroides forsythus in subgingival plaque samples obtained from 25 sudanese untreated adult periodontitis patients. the stand ... | 1994 | 7853129 |
| microbiota of rapidly progressive periodontitis lesions in association with clinical parameters. | the microbial population in 73 rapidly progressive periodontitis (rpp) lesions in 10 young adults aged 25 to 35 years (5 males, 5 females) was studied in relation to the clinical parameters probing depth, bleeding on probing, and suppuration, which were recorded at the sampled sites. porphyromonas gingivalis was found to predominate (26.7%) in 73 periodontal lesions with clinical probing depth > 6 mm, followed by bacteroides forsythus (23.6%), and prevotella intermedia (15.7%). the prevalence of ... | 1994 | 7853132 |
| presence of treponema denticola and porphyromonas gingivalis in children correlated with periodontal disease of their parents. | considerable evidence exists suggesting that periodontal disease is due to the overgrowth of a finite number of specific bacteria such as porphyromonas gingivalis, actinobacillus actinomycetemcomitans, treponema denticola, bacteroides forsythus, and prevotella intermedia, among others. three of these organisms-p. gingivalis, t. denticola, and b. forsythus-can be easily detected in plaque samples by the hydrolysis of the synthetic trypsin substrate benzoyl-dl-arginine-naphthylamide (bana). the ai ... | 1994 | 7929977 |
| molecular detection of bacteroides forsythus in human periodontitis. | the usefulness of a digoxigenin-labeled genomic dna probe for the detection of subgingival bacteroides forsythus was examined. in addition, the arbitrarily primed polymerase chain reaction (ap-pcr) was used to delineate the genetic diversity of b. forsythus periodontal isolates. the dna probe detected 10(3) b. forsythus cells and yielded a strong signal at 10(4) cells. it reacted with b. forsythus atcc 43037t and 44 clinical isolates and showed no detectable reactivity with 75 strains of 24 othe ... | 1994 | 7936721 |
| macaca fascicularis as a model in which to assess the safety and efficacy of a vaccine for periodontitis. | we have assessed macaca fascicularis as a potential model in which to test the efficacy and safety of a vaccine for periodontitis. twenty-eight animals were surveyed and 20 studied in more detail. clinical periodontal status was assessed, the subgingival microflora analyzed especially for the presence and proportions of porphyromonas gingivalis and titers and avidities of serum antibodies reactive with p. gingivalis measured. probing depths ranged from 0.90 mm to 3.80 mm, gingival index scores f ... | 1994 | 8008428 |
| assessment of risk of periodontal disease. | the periodontal disease research center at the state university of new york at buffalo has studied two sets of subjects--those with a high prevalence of diabetes (the pima indians) and an urban population in erie county, new york--to determine factors that put a patient at risk for periodontal disease. these and other studies have established that smoking and diabetes are important risk indicators for periodontal disease. local etiologic factors associated with periodontal disease in adults incl ... | 1994 | 8039205 |
| interdental supragingival plaque--a natural habitat of actinobacillus actinomycetemcomitans, bacteroides forsythus, campylobacter rectus, and prevotella nigrescens. | the aim of this study was to test the hypothesis that suspected periodontal pathogens form a minor component of the supragingival plaque of individuals without periodontal diseases. twenty-one dental hygienist trainees with a mean age of 23.5 years were twice sampled for interdental plaque between 1st and 2nd molars in all quadrants. the samples were assessed for actinobacillus actinomycetemcomitans, bacteroides forsythus, campylobacter rectus, porphyromonas gingivalis, and the prevotella interm ... | 1994 | 8083438 |
| assessment of risk for periodontal disease. i. risk indicators for attachment loss. | specific risk indicators associated with either susceptibility or resistance to severe forms of periodontal disease were evaluated in a cross-section of 1,426 subjects, 25 to 74 years of age, mostly metropolitan dwellers, residing in erie county, new york, and surrounding areas. the study sample exhibited a wide range of periodontal disease experience defined by different levels of attachment loss. therefore, it was possible to accurately assess associations between the extent of periodontal dis ... | 1994 | 8164120 |
| the effects of incubation length and temperature on the specificity and sensitivity of the bana (n-benzoyl-dl-arginine-naphthylamide) test. | a previous multi-center study examining patients diagnosed as having at least four periodontally diseased teeth showed that when bana (n-benzoyl-dl-arginine-naphthylamide) hydrolysis by periodontal pathogens such as treponema denticola, porphyromonas gingivalis, and bacteroides forsythus was evaluated versus clinical parameters such as clinical judgment of disease, bleeding on probing, and pocket depth, the sensitivity of the test was 84%, 82%, and 87%, respectively, while the specificity was on ... | 1993 | 8229620 |
| phylogeny of bacteroides, prevotella, and porphyromonas spp. and related bacteria. | the phylogenetic structure of the bacteroides subgroup of the cytophaga-flavobacter-bacteroides (cfb) phylum was examined by 16s rrna sequence comparative analysis. approximately 95% of the 16s rrna sequence was determined for 36 representative strains of species of prevotella, bacteroides, and porphyromonas and related species by a modified sanger sequencing method. a phylogenetic tree was constructed from a corrected distance matrix by the neighbor-joining method, and the reliability of tree b ... | 1994 | 8300528 |
| oral and dental infections with anaerobic bacteria: clinical features, predominant pathogens, and treatment. | microbial populations colonizing the teeth are a major source of pathogens responsible for oral and dental infections, including periodontal diseases, gingivitis, pericoronitis, endodontitis, peri-implantitis, and postextraction infections. each entity has distinct clinical and microbial features. bacterial species associated with oral infections include actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, bacteroides forsythus, campylobacter rectus, eubacterium ... | 1993 | 8324136 |
| effects of antibiotic treatment on clinical conditions and bacterial growth with guided tissue regeneration. | mucogingival flaps were reflected over pairs of mandibular molar teeth with class ii furcation invasions. the dimensions of the furcations were measured. the teeth were debrided and an expanded polytetrafluoroethylene (e-ptfe) membrane was placed and retained over one furcation of each pair (test site) for 4 weeks. the second site served as a control. eight patients (group 1) with 12 e-ptfe sites received no antibiotic. seven patients (group 2) with 12 e-ptfe sites were administered amoxicillin/ ... | 1993 | 8366411 |
| the effect of incubation temperature on the specificity of the bana (n-benzoyl-dl-arginine-naphthylamide) test. | the hydrolysis of bana by subgingival plaque samples is associated with the presence of either treponema denticola, porphyromonas gingivalis, and/or bacteroides forsythus. a protocol in which pure cultures were incubated for 15 min at 55 degrees c detected about 5 x 10(5) cfu of p. gingivalis and 1 x 10(6) cfu of t. denticola. clinical studies indicated that the bana test in this configuration will detect about 10(4) organisms in vivo as compared with the 10(5) to 10(6) organisms found with in v ... | 1993 | 8390044 |
| macaca nemestrina: a non-human primate model for studies of periodontal disease. | the non-human primate macaca nemestrina was evaluated for use as a potential model in periodontal research by study of 16 animals. using one incisor, premolar, and molar per quadrant, we measured supragingival plaque, severity of gingival inflammation, and pocket depth, and analyzed the subgingival flora. serum igg titers and avidities to antigens of porphyromonas gingivalis (pg) and actinobacillus actinomycetemcomitans (aa) were also assessed. ten animals were between 13 and 24 years old, and s ... | 1993 | 8393106 |
| effect of therapy on periodontal infections. | periodontal disease progression requires the simultaneous presence of high numbers of pathogens, low numbers of compatible or beneficial species, a conductive local environment, and a susceptible host. effective therapy acts by altering one or more of these factors. data from an ongoing study were used to examine the biological basis of treatment success or failure. seventeen subjects showing disease progression were treated by widman flap surgery at deep sites, scaling at shallow sites, and 1 o ... | 1993 | 8410615 |
| microbial composition and pattern of antibiotic resistance in subgingival microbial samples from patients with refractory periodontitis. | a series of 993 subgingival microbial samples sent to a diagnostic microbiology laboratory included 196 samples that could be identified as compatible with a clinical diagnosis of refractory or recurrent periodontitis. in descending order of prevalence the associated microbiota included bacteroides forsythus (84%), spirochetes (83%), motile rods (76%), fusobacterium species (68%), porphyromonas gingivalis (63%), campylobacter rectus (47%), capnocytophaga species (38%), prevotella intermedia (23% ... | 1993 | 8463936 |
| aspartame as a source of essential phenylalanine for the growth of oral anaerobes. | phenylalanine and aspartic acid requirements were determined for 13 species of oral bacteria using the chemically defined medium omiz-w1. none of actinobacillus actinomycetemcomitans, bacteroides forsythus, eikenella corrodens, selenomonas sputigena, treponema pectinovorum, t. socranskii, or wolinella recta required either of these amino acid constituents of aspartame (l-aspartyl-l-phenylalanine methylester). phenylalanine was essential for the growth of capnocytophaga gingivalis, eubacterium ti ... | 1993 | 8514113 |
| microbial alterations in supragingival dental plaque in response to a triclosan-containing dentifrice. | a total of 325 subjects were entered into a double-blind, stratified 2-treatment clinical study that examined the effects of a dentifrice containing 0.3% triclosan, 2% gantrez copolymer and 0.243% sodium fluoride on supragingival dental plaque and gingivitis. a subset of 159 subjects including 72 men and 87 women participated in the microbiological component of this study, which was designed to detect shifts in supragingival bacterial species in response to triclosan. subjects were divided into ... | 1995 | 8602338 |
| interbacterial binding among strains of pathogenic and commensal oral bacterial species. | strong interspecies adherence was demonstrated among the periodontal pathogens treponema denticola, bacteroides forsythus and porphyromonas gingivalis, and between these pathogens and the commensal plaque organism streptococcus crista. adherence showed specificity and demonstrated saturation binding kinetics. binding between b. forsythus and p. gingivalis appeared to be a unimodal protein-protein interaction. binding between the other organisms was at least bimodal involving interactions between ... | 1996 | 8604253 |
| bacteriological diagnosis of periodontal disease. | dental plaque bacteria cause virtually all the forms of inflammatory periodontal disease. periodontitis is caused by the specific periodontopathic bacteria, which induce destruction of connective tissue attachment and adjacent alveolar bone. examinations to identify the infections by porphyromonas gingivalis, actinobacillus actinomycetemcomitans, prevotella intermedia, campylobacter rectus, bacteroides forsythus, fusobacterium nucleatum, eikenella corrodens and treponema denticola have recently ... | 1994 | 8620588 |
| comparison of randomly cloned and whole genomic dna probes for the detection of porphyromonas gingivalis and bacteroides forsythus. | whole genomic and randomly-cloned dna probes for two fastidious periodontal pathogens, porphyromonas gingivalis and bacteroides forsythus were labeled with digoxigenin and detected by a colorimetric method. the specificity and sensitivity of the whole genomic and cloned probes were compared. the cloned probes were highly specific compared to the whole genomic probes. a significant degree of cross-reactivity with bacteroides species, capnocytophaga sp. and prevotella sp. was observed with the who ... | 1996 | 8636873 |
| effect of initial therapy on dynamics of immunogloblin g levels to some periodontopathic bacteria in serum and gingival crevicular fluid. | using enzyme-linked immunosorbent assay, the igg antibody levels to specific gram-negative periodontopathic bacteria in serum of 21 adult periodontitis patients and the gingival crevicular fluid (gcf) from 42 sites with destructive lesions and 21 healthy sites were evaluated before and after initial preparation. porphyromonas gingivalis, bacteroides forsythus, prevotella intermedia, prevotella nigrescens, campylobacter rectus, treponema denticola and actinobacillus actinomycetemcomitans in subgi ... | 1994 | 8689734 |
| microbial flora in the acute phase of periodontitis and the effect of local administration of minocycline. | periodontitis, similar to other infectious diseases, is known to progress as chronic inflammation with recurrent acute phases. the purpose of this study was to clarify the microbiological composition of the acute phase and to compare the bacterial flora with that of comparable chronic periodontal pockets. we also evaluated the effect of application of minocycline gel locally on the change in the microflora in the acute pockets. microbial flora from the subgingival pockets of 28 patients in the a ... | 1996 | 8708969 |
| long-term stability of class ii furcation defects treated with barrier membranes. | the present longitudinal study was designed to explore the long-term efficacy of guided tissue regeneration (gtr) in class ii furcation defects and establish the factors that might be responsible for modifying this response. subjects with two or more mandibular molars, one of which had class ii furcation defects, received the hygienic phase of therapy followed by baseline clinical measurements and subgingival plaque sampling. gtr procedure was performed in furcation defect sites using expanded p ... | 1996 | 8724711 |
| polyphasic analysis of strains of the genus capnocytophaga and centers for disease control group df-3. | a polyphasic approach was used to determine the relationships between well-characterized reference strains representing all seven capnocytophaga species. one centers for disease control (cdc) group df-3 strain, a presumed relative of the genus capnocytophaga, and 15 field isolates were included as well. fourteen isolates were assigned to named capnocytophaga species, all of which could be differentiated by means of whole-organism protein electrophoresis. a separate position was occupied by the c ... | 1996 | 8782690 |
| rapid characterization of periodontal bacterial isolates by using fluorogenic substrate tests. | eighty-nine species of subgingival bacteria, represented by 121 reference strains and 892 patient isolates, including gram-negative, gram-positive, aerobic, facultatively anaerobic, microaerophilic, and anaerobic species, were characterized with a panel of fluorogenic, 4-methylumbelliferyl-linked substrate tests. identifications of all patient isolates were confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of whole-cell proteins relative to reference strains. char ... | 1996 | 8789019 |
| prevalence of 6 putative periodontal pathogens in subgingival plaque samples from romanian adult periodontitis patients. | the aim of the present study was to determine by standard cultivation procedures the detection frequencies of porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, actinobacillus actinomycetemcomitans, bacteroides forsythus, and capnocytophaga species as well as various enteric rods in subgingival plaque samples form romanian adult periodontitis patients. dna probe analysis (affirm dp microbial identification test) was also used, parallel to cultivation, to identify p. gingiv ... | 1996 | 8849850 |
| clinical/biological outcomes of treatment for pericoronitis. | this prospective clinical study was designed to determine the clinical and biologic outcomes of treatment for minor signs and symptoms of pericoronitis. | 1996 | 8859232 |
| short-term responses to periodontal therapy in insulin-dependent diabetic patients. | this investigation studied relative changes in periodontal conditions of 18 insulin-dependent diabetic patients. measures of gingival inflammation, crevicular fluid aspartate aminotransferase (ast) levels, probing depth and attachment levels, the presence of three periodontal pathogens (porphyromonas gingivalis, bacteroides forsythus, and actinobacillus actinomycetemcomitans) and serum antibody titers to these bacteria, and blood sugar levels (glycosylated hemoglobin, hbalc) were studied before ... | 1996 | 8866319 |
| cigarette smoking increases the risk for subgingival infection with periodontal pathogens. | cigarette smoking has been found to increase the risk for periodontitis. the present study examined the association between cigarette smoking and subgingival infection with periodontal pathogens to determine if smokers are more likely to be infected with certain periodontal pathogens than non-smokers. self-reported data on 1,426 subjects, aged 25 to 74, from the erie county study were obtained including data on 798 subjects who were current or former smokers. mean clinical attachment loss was us ... | 1996 | 8910822 |
| exploratory case-control analysis of psychosocial factors and adult periodontitis. | we explored the association between social factors and adult periodontitis by comparing self-reported information for daily strains and symptoms of depression in 71 cases and 77 controls. cases and controls were selected from among 1,426 participants in the erie county risk factor study. we found differences among those who scored higher than their peers on measures of social strain. the odds ratio (or) and 95% confidence interval (95% ci) for the association between case status and role strain ... | 1996 | 8910824 |
| [evaluation of non radioactive dna probe (affirm dp) for detecting periodontopathic bacteria]. | the purpose of this investigation was to evaluate the potential of application of non-radioactive dna probe method (affirm dp) in clinical diagnosis and evaluation of treatment efficacy. sensitivity and specificity of two different cut-off levels (10(4), 5 x 10(5)) of the method with bacterial culture in detection of bacteroides forsythus, porphyromonas gingivalis, and actinobacillus actinomycetemcomitans were compared. those bacteria were obtained from bacterial cultures and clinical samples fr ... | 1996 | 8921689 |
| selling biotechnology in the dental medicine marketplace: the omnigene diagnostics dna probe tests for periodontal pathogens. | omnigene diagnostics, inc. has applied the principles of genetic engineering to develop species-specific dna probe tests for eight periodontal pathogens (porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetem-comitans, fusobacterium nucleatum, eikenella corrodens, campylobacter rectus, bacteroides forsythus, and treponema denticola). the test requires minimal effort on the part of the clinician: subgingival plaque samples are collected from the patient and sent through the ... | 1996 | 8931245 |
| degradation of host protease inhibitors and activation of plasminogen by proteolytic enzymes from porphyromonas gingivalis and treponema denticola. | bacterial proteases may participate in the pathogenesis of periodontal diseases through their action on host proteins. in the present study, the ability of selected periodontopathogens, as well as two proteases isolated from porphyromonas gingivalis and treponema denticola, to degrade host protease inhibitors was evaluated. the activation of human plasminogen by the two bacterial proteases was also investigated. proteolytic breakdown of host protease inhibitors (alpha-1-antitrypsin, antichymotry ... | 1996 | 8936322 |
| degradation of lactoferrin by periodontitis-associated bacteria. | the degradation of human lactoferrin by putative periodontopathogenic bacteria was examined. fragments of lactoferrin were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and measured by densitometry. the degradation of lactoferrin was more extensive by porphyromonas gingivalis and capnocytophaga sputigena, slow by capnocytophaga ochracea, actinobacillus actinomycetemcomitans and prevotella intermedia, and very slow or absent by prevotella nigrescens, campylobacter rectus ... | 1996 | 8961558 |
| shared antigens of porphyromonas gingivalis and bacteroides forsythus. | periodontitis in humans is caused by a group of predominantly gram-negative, anaerobic bacteria among which porphyromonas gingivalis and bacteroides forsythus are prominent. a similar group is present and presumably plays a similar role in experimental periodontitis in the primate macaca fascicularis. nevertheless, immunization using a vaccine containing only killed p. gingivalis suppresses the progress of experimental periodontitis in m. fascicularis. we investigated the hypothesis that gram-ne ... | 1996 | 9002874 |
| polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions. | a 16s rrna-based polymerase chain reaction (pcr) detection method was used to determine the prevalence of actinobacillus actinomycetemcomitans, bacteroides forsythus, campylobacter rectus, eikenella corrodens, porphyromonas gingivalis, prevotella intermedia. prevotella nigrescens and treponema denticola in subgingival specimens of 50 advanced periodontitis, 50 adult gingivitis and 50 pediatric gingivitis subjects. the optimal pcr conditions were determined for each study species. agarose gel ele ... | 1996 | 9002880 |
| a pcr-dna probe assay specific for bacteroides forsythus. | bacteroides forsythus is a fastidious anaerobic gram-negative organism associated with active periodontal disease. the ability of random amplified polymorphic dna (rapd) fingerprinting to generate species-specific markers was exploited towards the construction of a polymerase chain reaction (pcr)-dna probe assay specific for b. forsythus. the strategy included the four following steps: (1) construction of a first generation dna probe based on a 507-bp rapd species-specific marker; (2) cloning an ... | 1996 | 9025078 |
| occurrence of porphyromonas gingivalis, bacteroides forsythus, and treponema denticola in periodontally healthy and diseased subjects as determined by an elisa technique. | the aim of this study was to assess by means of an elisa technique, the occurrence of 3 putative periodontopathogens, porphyromonas gingivalis, bacteroides forsythus, and treponema denticola, in 3 clinically-defined adult periodontal conditions. thirty systemically-healthy subjects were selected and grouped into 3 categories according to their periodontal health: 1) 10 periodontitis subjects (ps), having moderate adult chronic periodontitis; 2) 10 untreated gingivitis subjects (ugs), exhibiting ... | 1997 | 9029447 |
| experimental peri-implant tissue breakdown around hydroxyapatite-coated implants. | this study monitored experimental peri-implant tissue breakdown around hydroxyapatite (ha)-coated titanium dental implants. thirty-two ha-coated cylindrical implants, in groups of two, were bilaterally inserted in the posterior maxilla and mandible in 4 macaca mulatta monkeys. two months after healing-abutment connection, a 2-month plaque control program was initiated. clinical and radiographic recordings and peri-implant submucosal microbial samples were then obtained (baseline). cotton ligatur ... | 1997 | 9029453 |
| rapid diagnosis of periodontal infections: findings in aids patients. | a small number of bacterial pathogens in the human oral cavity cause the different forms of periodontal disease. of the approximately two hundred different oral bacterial species, about a dozen have been associated with these diseases including localized juvenile periodontitis, rapidly progressing periodontitis, and adult periodontitis. these species include actinobacillus actinomycetemcomitans, bacteroides forsythus, campylobacter rectus, porphyromonas gingivalis, and prevotella intermedia. sev ... | 1997 | 9037612 |
| detection of peptidase activity from treponema denticola, porphyromonas forsythus, and bacteroides forsythus as a means of periodontal therapy evaluation. | we have previously reported that the periocheck test offers a rapid, simple periodontal diagnostic tool by detecting peptidase activity specifically attributed to treponema denticola, porphyromonas gingivalis, and bacteroides forsythus. relationships between the peptidase activity detected by the periocheck test and clinical measures were investigated. fifty-seven periocheck-positive sites selected from 27 patients with adult periodontitis were subjected to scaling and root planing. two weeks la ... | 1995 | 9055698 |
| longitudinal study of prognostic factors in established periodontitis patients. | numerous indicators for disease progression have been described in the last decade. the purpose of this study was to examine, longitudinally, a large battery of clinical, microbiological, and immunological indicators, to try to determine whether the presence of one or a combination of these parameters at baseline, would correlate positively with increased attachment and or bone loss (true prognostic factors). following initial screening, 79 patients with established periodontitis were monitored ... | 1997 | 9062856 |
| periodontal bacteria colonizing oral mucous membranes in edentulous patients wearing dental implants. | the purpose of this study was to investigate the prevalence of the microbiota on the oral mucosal surfaces and in the peri-implant pocket in edentulous subjects with a past history of periodontitis. twenty edentulous subjects wearing dental implants for at least one year and with a history of periodontitis participated in this study. clinical parameters were assessed (plaque, redness, swelling, peri-implant probing depth [pipd], and bleeding on probing) and microbiological samples were taken (or ... | 1997 | 9100195 |
| relationship between peptidase activity from treponema denticola, porphyromonas gingivalis, and bacteroides forsythus and attachment loss. | as a diagnostic test, "periocheck" can detect the n-carbobenzoxyglycyl-glycy-arginyl peptidase that is produced by treponema denticola, porphyromonas gingivalis, and bacteroides forsythus. the aim of this study was to clarify the relationship between peptidase activity and attachment loss. after phase 1 and surgical therapy, a total of 111 sites from 47 adult periodontitis patients were divided into four groups according to peptidase activity (trypsin unit, tu): a, < 0.1 tu; b, 0.1-0.2 tu; c and ... | 1996 | 9116469 |
| effects of smoking and smoking cessation on healing after mechanical periodontal therapy. | this study investigated the effect of cigarette smoking on 143 patients' clinical and microbiological responses to mechanical therapy. treatment included four to six sessions of subgingival scaling and root planing and instruction in oral hygiene. results indicate that current smokers have less healing and reduction in subgingival bacteroides forsythus and porphyromonas gingivalis after treatment compared to former and nonsmokers, suggesting that smoking impairs periodontal healing. as the heali ... | 1997 | 9150643 |
| characterization of bacteroides forsythus isolates. | fifteen bacteroides forsythus strains freshly isolated from patients with periodontitis were used together with three collection strains and one type strain for characterization of growth on various media; determination of enzymatic profiles, antibiotic susceptibility profiles, 16s rrna ribotypes, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) outer membrane protein profiles, and pathogenicity; and gas chromatography analysis by using a wound chamber model in rabbits. all s ... | 1997 | 9163447 |
| periodontal findings in cohen syndrome with chronic neutropenia. | radiographic periodontal status and microbiological findings of periodontal pockets in subjects with cohen syndrome are presented in this report. this hereditary disorder causes mental retardation, and neutropenia is one feature of the syndrome. fifteen patients with cohen syndrome and 15 controls matched for age and sex and, as far as possible, according to the degree of mental retardation were examined. alveolar bone loss was evaluated from the panoramic radiographs. two subgingival samples we ... | 1997 | 9182743 |
| identification of bacteroides forsythus in subgingival dental plaque with the aid of a rapid pcr method. | bacteroides forysthus has been shown to be prevalent among patients with periodontitis. conventional microbiological methods used to identify this bacterium, however, are laborious and time-consuming and are therefore not well-suited for screening purposes. we have developed a polymerase chain-reaction (pcr) method which is rapid, specific, and simple to perform and does not require other sample pre-treatment except a brief centrifugation. this method was applied to the detection of b. forsythus ... | 1997 | 9207770 |
| occurrence of invading bacteria in radicular dentin of periodontally diseased teeth: microbiological findings. | bacterial invasion in roots of periodontally diseased teeth, which has been recently documented using cultural and microscopic techniques, may be important in the pathogenesis of periodontal disease. the purpose of this investigation was to determine the occurrence and the species of invading bacteria in radicular dentin of periodontally diseased teeth. samples were taken from the middle layer of radicular dentin of 26 periodontally diseased teeth. 14 healthy teeth were used as controls. dentin ... | 1997 | 9226388 |
| subgingival microbiota in adult chinese: prevalence and relation to periodontal disease progression. | the "checkerboard" dna-dna hybridization technology was used to study the epidemiology of 18 microbial species associated with various states of periodontal health and disease, in a sample of 148 chinese subjects never exposed to systematic dental therapeutic intervention, aged 30 to 39 and 50 to 59 years. our aims were to: 1) describe the prevalence of these microorganisms; 2) correlate the microbiological and clinical profiles of the subjects; and 3) examine the association between the microbi ... | 1997 | 9249637 |
| beta-lactamase producing bacteria in adult periodontitis. | in 23 untreated adult periodontitis patients, the occurrence of beta-lactamase producing periodontal bacteria was determined. in addition to non-selective isolation media, selective isolation and growth of beta-lactamase positive subgingival bacterial species was carried out on blood agar plates supplemented with amoxicillin and plates with amoxicillin+clavulanic acid. porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetemcomitans, peptostreptococcus micros, fusobacterium ... | 1997 | 9266340 |
| effects of metronidazole in patients with "refractory" periodontitis associated with bacteroides forsythus. | the aim of the present study was to monitor the microbiological and clinical effects of renewed supra- and subgingival debridement in conjunction with systemic metronidazole therapy (500 mg tid for 7 days) in 27 "refractory" periodontitis patients, culture positive for bacteroides forsythus and negative for actinobacillus actinomycetemcomitans. clinical evaluation included assessment of plaque, bleeding upon probing, probing pocket depth and clinical attachment loss at the deepest, bleeding site ... | 1997 | 9266345 |
| assessment of pretreatment orthodontic patients using the bana test. n-benzoyl-dl-arginine-naphthylamide. | ninety-two subjects were tested before orthodontic mechanotherapy for the presence of three putative periodontopathogens-porphyromonas gingivalis, treponema denticola, and bacteroides forsythus-with the bana test (perioscan oral-b). the sample ranged from 9 to 16 years, with a mean age of 13.1 years, and consisted of 51% girls and 49% boys; 48% african american, 46% white, 4% asian, and 2% hispanic. unlike other bana studies that examined children and adolescents with a 15-minute incubation time ... | 1997 | 9267226 |
| the survival rate of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and bacteroides forsythus following 4 randomized treatment modalities. | the overall goal of this clinical study was to determine the short-term anti-infective effects of four randomized treatment modalities on actinobacillus actinomycetemcomitans (aa), porphyromonas gingivalis (pg), and bacteroides forsythus (bf) and determine the effects of bacterial survival on treatment outcomes in patients with adult periodontitis. twelve adult patients requiring therapy for moderate periodontitis were selected for this study. all patients had at least one tooth in each quadrant ... | 1997 | 9287061 |
| a comparison of igg antibody reactive with bacteroides forsythus and porphyromonas gingivalis in adult and early-onset periodontitis. | recent work has indicated that bacteroides forsythus and porphyromonas gingivalis are significant local risk factors for periodontitis. several reports find that both organisms are frequently associated with periodontitis lesions and often are present together. we have previously shown that early-onset periodontitis patients seropositive for p. gingivalis have less attachment loss than seronegative patients. in this study, we determined serum igg antibody concentrations reactive with b. forsythu ... | 1997 | 9287063 |
| dental implant infections. | dental implants provide a restorative tool to support crowns, bridge abutments, and removable dentures. osseointegrated implants are titanium posts that are surgically implanted in alveolar bone. a tight immobile bond (osseointegration) forms between bone and titanium, and prosthetic and restorative fixtures are attached to the implants. titanium implants differ from natural teeth, which may make them more susceptible to mechanical stress. a small proportion of implants are not successful and ma ... | 1997 | 9310682 |