| phylogeny of fast-growing soybean-nodulating rhizobia support synonymy of sinorhizobium and rhizobium and assignment to rhizobium fredii. | we determined the sequences for a 260-base segment amplified by the polymerase chain reaction (corresponding to positions 44 to 337 in the escherichia coli 16s rrna sequence) from seven strains of fast-growing soybean-nodulating rhizobia (including the type strains of rhizobium fredii chemovar fredii, rhizobium fredii chemovar siensis, sinorhizobium fredii, and sinorhizobium xinjiangensis) and broad-host-range rhizobium sp. strain ngr 234. these sequences were compared with the corresponding pre ... | 1992 | 1371066 |
| identification of isolates from soybean nodules in xinjiang region as sinorhizobium xinjiangense and genetic differentiation of s. xinjiangense from sinorhizobium fredii. | eight fast-growing rhizobial isolates from xinjiang soils were identified as sinorhizobium xinjiangense by analyses of 16s rrna gene sequences, sds-page of proteins, intergenic spacer sequences and dna-dna hybridization. based on all of the results, these isolates and the reference strains for s. xinjiangense were a distinct genomic species, although the 16s rrna genes were closely related to that of sinorhizobium fredii. | 2002 | 11931157 |
| [analysis on 16s rdna sequence of rhizobia isolated from kummerowla sp]. | based on the previous studies on numerical taxonomy, sds-page of whole-cell protein and dna hybridization, the rhizobial strains isolated from kummerowia sp. in semi-arid area of north-west constituted a new subgroup, the 16s rdna sequence of representative strain sh714 were tested. the unrooted phylogenetic tree was produced. in this tree, the strain sh714 with sinorhizobium xinjiangensis, s. fredii, s. meliloti, s. medicae, s. saheli and s. teranga constituted a branch of sinorhizobium. within ... | 2001 | 12549200 |
| the genus name ensifer casida 1982 takes priority over sinorhizobium chen et al. 1988, and sinorhizobium morelense wang et al. 2002 is a later synonym of ensifer adhaerens casida 1982. is the combination "sinorhizobium adhaerens" (casida 1982) willems et al. 2003 legitimate? request for an opinion. | the synonymy of the genera ensifer and sinorhizobium was recently reported, but it was proposed that the later-named genus, sinorhizobium, take priority in nomenclature. there is no justification in the international code of nomenclature of bacteria (prokaryotes) for this step; ensifer is the correct name of the genus, with ensifer adhaerens as the type species. species previously allocated to sinorhizobium are here proposed as the new combinations ensifer arboris, ensifer fredii, ensifer kostie ... | 2003 | 14657154 |
| identification of fast and slow growing rhizobia nodulating soybean (glycine max [l.] merr) by a multiplex pcr reaction. | two dna fragments, a 730-bp and a 900-bp fragment, one homologous to host cultivar specificity genes nolbt of sinorhizobium fredii and the other one homologous to rsalpha, an insertion-like sequence present in bradyrhizobium japonicum, were generated by polymerase chain reaction (pcr) with two pairs of primers. the amount of each fragment generated by the multiplex pcr was proportional to the amount of template dna present. the amplification of the 900-bp rsalpha fragment was more sensitive, sin ... | 2003 | 14680692 |
| advantages of multilocus sequence analysis for taxonomic studies: a case study using 10 housekeeping genes in the genus ensifer (including former sinorhizobium). | there is a need for easy, practical, reliable and robust techniques for the identification and classification of bacterial isolates to the species level as alternatives to 16s rrna gene sequence analysis and dna-dna hybridization. here, we demonstrate that multilocus sequence analysis (mlsa) of housekeeping genes is a valuable alternative technique. an mlsa study of 10 housekeeping genes (atpd, dnak, gap, glna, glta, gyrb, pnp, reca, rpob and thrc) was performed on 34 representatives of the genu ... | 2008 | 18175710 |