purification and partial characterization of the rhizobium leguminosarum biovar viciae ca2+-dependent adhesin, which mediates the first step in attachment of cells of the family rhizobiaceae to plant root hair tips.the ca2+-dependent adhesin which mediates the first step in attachment of bacteria of the family rhizobiaceae to plant root hair tips was isolated from the surface of rhizobium leguminosarum biovar viciae cells; its ability to inhibit attachment of r. leguminosarum to pea root hair tips was used as a bioassay. isolated adhesin was found to be able to inhibit attachment of both carbon-limited and manganese-limited r. leguminosarum cells. a multicolumn purification procedure was developed which re ...19892738027
expression of tfx and sensitivity to the rhizobial peptide antibiotic trifolitoxin in a taxonomically distinct group of alpha-proteobacteria including the animal pathogen brucella abortus.three phylogenetically distinct groups within the alpha-proteobacteria which differ in trifolitoxin sensitivity are described. trifolitoxin sensitivity was found in strains of agrobacterium, brucella, mycoplana, ochrobactrum, phyllobacterium, rhodobacter, rhodopseudomonas, rhodospirillum, and rhizobium. strains of agrobacterium, brucella, phyllobacterium, rhizobium, and rhodospirillum were capable of producing trifolitoxin upon conjugal transfer of tfxabcdefg.19947527627
specificity of six gene sequences for the detection of the genus brucella by dna amplification.dna extracted from all brucella species, reference and vaccine strains were amplified by pcr using primers specific for the genes encoding a 31-kda brucella protein, the heat shock proteins (dnaj, dnak, htra and groel) and 16s rna. no difference was found between brucella species and biovars with all primer pairs used, even after restriction enzyme analysis of the amplified fragments. the specificity of the amplified products was confirmed by hybridization with a digoxigenin 3'-labelled specific ...19968810054
brucellosis: an overview.brucellosis remains a major zoonosis worldwide. although many countries have eradicated brucella abortus from cattle, in some areas brucella melitensis has emerged as a cause of infection in this species as well as in sheep and goats. despite vaccination campaigns with the rev 1 strain, b. melitensis remains the principal cause of human brucellosis. brucella suis is also emerging as an agent of infection in cattle, thus extending its opportunities to infect humans. the recent isolation of distin ...19979204307
bacillary angiomatosis: a role for phyllobacterium? 19979233469
localization and characterization of a specific linear epitope of the brucella dnak protein.we have previously produced and characterized four monoclonal antibodies to the brucella dnak protein which were derived from mice infected with b. melitensis or immunized with the b. melitensis cell wall fraction. by use of a recombinant dna technique, we have localized a linear epitope, recognized by two of these monoclonal antibodies (v78/07b01/g11 and v78/09d04/d08), in the last 21 amino acids of the c-terminal region of the brucella dnak protein. the c-terminal region has been reported to b ...19979297829
characterization of aquamicrobium defluvii gen. nov. sp. nov., a thiophene-2-carboxylate-metabolizing bacterium from activated sludge.a gram-negative bacterium was isolated from activated sewage sludge with thiophene-2-carboxylate as the sole source of carbon and with nitrate as an electron acceptor. the isolate, strain nkk, was a motile, oxidase- and catalase-positive, rod-like bacterium with a g+c content of 61.7 mol%. besides nitrate, oxygen could serve as a terminal electron acceptor. among many carbon sources tested, only a few sugars, fatty acids, and thiophene-2-carboxylate supported growth. other heterocyclic compounds ...19989531630
unconventional genomic organization in the alpha subgroup of the proteobacteria.pulsed-field gel electrophoresis was used to analyze the genomic organization of 16 bacteria belonging or related to the family rhizobiaceae of the alpha subgroup of the class proteobacteria. the number and sizes of replicons were determined by separating nondigested dna. hybridization of an rrn gene probe was used to distinguish between chromosomes and plasmids. members of the genus agrobacterium all possess two chromosomes, and each biovar has a specific genome size. as previously demonstrated ...19989573163
defluvibacter lusatiae gen. nov., sp. nov., a new chlorohenol-degrading member of the alpha-2 subgroup of proteobacteria.the two gram-negative bacterial strains s1 and s4 were isolated from activated sludge of an industrial waste water treatment plant and exhibited a stable capability to degrade 2,4-dichlorophenol, 4-chloro-2-methylphenol, 4-chlorophenol and phenol. the cells were short rods with a polar flagellum, being mesophilic, strictly aerobic, oxidase-positive, and chemoorganotrophic. they utilized a range of amino acids, but only a restricted number of carbohydrates. reassociation experiments with dna from ...199910390870
brucella outer membrane lipoproteins share antigenic determinants with bacteria of the family rhizobiaceae.brucellae have been reported to be phylogenetically related to bacteria of the family rhizobiaceae. in the present study, we used a panel of monoclonal antibodies (mabs) to brucella outer membrane proteins (omps) to determine the presence of common omp epitopes in some representative bacteria of this family, i.e., ochrobactrum anthropi, phyllobacterium rubiacearum, rhizobium leguminosarum, and agrobacterium tumefaciens, and also in bacteria reported to serologically cross-react with brucella, i. ...199910391877
changes in the metabolism of the microalga chlorella vulgaris when coimmobilized in alginate with the nitrogen-fixing phyllobacterium an agroindustrial wastewater pond, a naturally occurring unicellular microalga, chlorella vulgaris, was closely associated with the terrestrial plant-associative n2-fixing bacterium phyllobacterium myrsinacearum. when the two microorganisms were artificially coimmobilized in alginate beads, they shared the same internal bead cavities, and the production of five microalgal pigments increased, but there were no effects on the number of the cells or the biomass of the microalga. the association, ...200010932359
synergism between phyllobacterium sp. (n(2)-fixer) and bacillus licheniformis (p-solubilizer), both from a semiarid mangrove rhizosphere.mangrove seedlings were treated with a mixture of two bacterial species, the slow-growing, n(2)-fixing bacterium phyllobacterium sp. and the fast-growing, phosphate-solubilizing bacterium bacillus licheniformis, both isolated from the rhizosphere from black, white, and red mangroves of a semiarid zone. nitrogen fixation and phosphate solubilization increased when the mixture was used compared to the effects observed when adding individual cultures, notwithstanding that there was no increase in b ...200111295457
microfloral diversity of cultured and wild strains of psoroptes ovis infesting sheep.pcr amplification of 16s rdna was used to determine the diversity of bacteria associated with 3 strains of sheep scab mite, psoroptes ovis. eight species of bacteria were identified by phylogenetic analysis of the pcr product sequences. seven of these species are previously unreported in association with sheep scab mites. five species were matched to serratia marcesens, proponibacteium acnes, phyllobacterium rubiacearum, pantoea agglomerans, curacaobacter baltica, whereas the remaining 3 sequenc ...200111719954
phyllobacterium myrsinacearum (subjective synonym phyllobacterium rubiacearum) emend.the taxonomic relationships between phyllobacterium myrsinacearum lmg 2t2t, phyllobacterium rubiacearum lmg 1t1t and two phyllobacterium strains representing a collection of isolates from sugar-beet roots were investigated by means of fatty acid analysis and dna-dna hybridization. the strains showed very similar fatty acid compositions and more than 96% dna reassociation to each other. on the basis of these results and available data from the literature, it is proposed that p. rubiacearum be cla ...200212361292
ultrastructure of interaction in alginate beads between the microalga chlorella vulgaris with its natural associative bacterium phyllobacterium myrsinacearum and with the plant growth-promoting bacterium azospirillum brasilense.chlorella vulgaris, a microalga often used in wastewater treatment, was coimmobilized and coincubated either with the plant growth-promoting bacterium azospirillum brasilense, or with its natural associative bacterium phyllobacterium myrsinacearum, in alginate beads designed for advanced wastewater treatment. interactions between the microalga and each of the bacterial species were followed using transmission electron microscopy for 10 days. initially, most of the small cavities within the beads ...200115049443
engineering root exudation of lotus toward the production of two novel carbon compounds leads to the selection of distinct microbial populations in the rhizosphere.the culture of opine-producing transgenic lotus plants induces the increase in the rhizosphere of bacterial communities that are able to utilize these molecules as sole carbon source. we used transgenic lotus plants producing two opines, namely mannopine and nopaline, to characterize the microbial communities directly influenced by the modification of root exudation. we showed that opine-utilizers represent a large community in the rhizosphere of opine-producing transgenic lotus. this community ...200415259274
nitrogen-fixing nodules from rose wood legume trees (dalbergia spp.) endemic to madagascar host seven different genera belonging to alpha- and beta-proteobacteria.although legume biodiversity is concentrated in tropical regions, the majority of studies on legume nodulating bacteria (lnb) are focused on cultivated leguminous plants from temperate regions. however, recent works on tropical regions tend to indicate that the actual diversity of lnb is largely underestimated. in this study, we report the isolation and characterization of 68 nitrogen-fixing root nodule bacteria collected from eight endemic tree species of dalbergia in madagascar. the isolates w ...200516262864
identification and plant interaction of a phyllobacterium sp., a predominant rhizobacterium of young sugar beet plants.the second most abundant bacterium on the root surface of young sugar beet plants was identified as a phyllobacterium sp. (rhizobiaceae) based on a comparison of the results of 39 conventional identification tests, 167 api tests, 30 antibiotic susceptibility tests, and sodium dodecyl sulfate-polyacrylamide gel electrophoretic fingerprints of total cellular proteins with type strains of phyllobacterium myrsinacearum and phyllobacterium rubiacearum. it was found on 198 of 1,100 investigated plants ...199016348158
fast-growing, aerobic, heterotrophic bacteria from the rhizosphere of young sugar beet, aerobic, heterotrophic bacteria from the root surface of young sugar beet plants were inventoried. isolation of the most abundant bacteria from the root surface of each of 1,100 plants between the second and tenth leaf stage yielded 5,600 isolates. these plants originated from different fields in belgium and spain. all isolates were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of total cellular proteins. comparison of protein fingerprints allowed us to ...199016348342
diverse bacteria associated with root nodules of spontaneous legumes in tunisia and first report for nifh-like gene within the genera microbacterium and starkeya.we characterized 34 endophytic bacterial isolates associated to root nodules collected from spontaneous legumes in the arid zone of tunisia by 16s rdna polymerase chain reaction (pcr)-restriction fragment length polymorphism, whole cell protein sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), 16s rdna and 16s-23s rdna internal transcribed spacer sequencing. phylogenetically, these isolates belong to the branches containing the genera inquilinus, bosea, rhodopseudomonas, para ...200616598639
utilization of hexamethylenetetramine (urotropine) by bacteria and yeasts.a slow growing bacterial population able to utilize hexamethylelenetetramine (urotropine) as sole source of carbon, nitrogen and energy was isolated from soil. from this crude enrichment culture two bacteria were isolated and identified as brevundimonas diminuta and a phyllobacterium sp. by sequencing of 16s ribosomal dna. these bacteria also grew on urotropine but at a lower rate than the enrichment culture. addition of glucose to the latter resulted in growth of some yeasts that overgrew the b ...200717043911
characterization of symbiotic and endophytic bacteria isolated from root nodules of herbaceous legumes grown in qinghai-tibet plateau and in other zones of china.qinghai-tibet plateau is the highest place in the world and the environment in that plateau is hard for animals and plants, with low temperature, low concentration of oxygen and high solar radiation. in this study, 61 root nodule isolates from vicia, oxytropis, medicago, melilotus and onobrychis species grown in qinghai-tibet plateau and in loess plateau were comparatively characterized. based upon the results of numerical taxonomy, ardra, aflp, dna-dna hybridization and 16s rdna sequencing, the ...200717541555
[bacteria closely related to phyllobacterium trifolii according to their 16s rrna gene are discovered in the nodules of hungarian sainfoin].the population genetic diversity and phylogeny of the bacteria entering the symbiosis with sainfoin that grows on the chesnokovskaya mountain, ufa region, republic of bashkortostan, have been studied. rapd analysis of dna polymorphism of the microbial strains grown from the nodules of 20 plants using several random primers detected a high degree of genetic homogeneity in their population as compared with the populations of rhizobia of other leguminous plants growing at the same site. sequencing ...200717633565
phenotypic and genotypic diversity of genista saharae microsymbionts from the infra-arid region of tunisia.aims: genista saharae, indigenous of sahara, is a spontaneous shrub that plays an important ecological role for the preservation and fertility of poor and eroded soils. this legume has not been examined for its root nodule bacteria. the taxonomic diversity of bacteria from root nodules of g. saharae growing in the infra-arid region of tunisia was investigated. methods and results: a total of 28 bacterial strains isolated from root nodules of g. saharae grown in tunisian soil were characterized u ...200717916130
[beta-proteorhizobia and nonrhizobial species--a review].the terms alpha- and beta-rhizobia were proposed to distinguish the rhizobial alpha- and beta-proteorhizobia respectively. in this review we present recently development of rhizobial taxonomy about beta-rhizobia in the genus burkholeria, cupriavidus isolated from mimosa and alpha-proteorhizobia belonging to methylobacterium, devosia, ochrobactrum, phyllobacterium and blastobacter lineage. furthermore, we also introduced the evolution of symbiosis related genes such as noda and nifh, and posed so ...200819160827
[endophytic bacterial diversity in glycyrrhiza inflata bat. from xinjiang by culture-independent method].we investigated endophytic bacterial diversity in glycyrrhiza inflata bat. from xinjiang.200919673406
ensifer, phyllobacterium and rhizobium species occupy nodules of medicago sativa (alfalfa) and melilotus alba (sweet clover) grown at a canadian site without a history of cultivation.phage-resistant and -susceptible bacteria from nodules of alfalfa and sweet clover, grown at a site without a known history of cultivation, were identified as diverse genotypes of ensifer, rhizobium and phyllobacterium species based on sequence analysis of ribosomal (16s and 23s rrna) and protein-encoding (atpd and reca) genes, southern hybridization/rflp and a range of phenotypic characteristics. among phage-resistant bacteria, one genotype of rhizobium sp. predominated on alfalfa (frequency ap ...201019875436
[isolation, identification and insecticidal activity of endophyte from achnatherum inebrians].to study endophyte species of achnatherum inebrians and to screen strains with insecticidal activity against cotton insect.201020560358
phaseolus vulgaris seed-borne endophytic community with novel bacterial species such as rhizobium endophyticum sp. nov.the bacterial endophytic community present in different phaseolus vulgaris (bean) cultivars was analyzed by 16s ribosomal rna gene sequences of cultured isolates derived from surface disinfected roots and immature seeds. isolated endophytes from tissue-macerates belonged to over 50 species in 24 different genera and some isolates from acinetobacter, bacillus, enterococcus, nocardioides, paracoccus, phyllobacterium, and sphingomonas seem to correspond to new lineages. phytate solubilizing bacteri ...201020822874
bacterial communities constructed in artificial consortia of bacteria and chlorella vulgaris.we established artificial consortia of bacteria inoculated from soil and a green alga, chlorella vulgaris nies-227. the bacteria and the alga were mixed in a tube or partitioned in a dialysing culture vessel, and the bacterial composition was examined after cultivation. the community of bacteria formed in the consortia included those phylogenetically closely related to the genera phenylobacterium, brevundimonas, phyllobacterium, afipia, sphingomonas, sandaracinobacter, ramlibacter, ralstonia, ce ...201021576850
genetic characterization of root-nodule bacteria associated with acacia salicina and a. stenophylla (mimosaceae) across south-eastern australia.symbiotic relationships between legumes and nitrogen-fixing soil micro-organisms are of ecological importance in plant communities worldwide. for example, nutrient-poor australian soils are often dominated by shrubby legumes (e.g. species of acacia). however, relatively few studies have quantified patterns of diversity, host-specificity and effectiveness of these ecologically important plant-microbe interactions. in this study, 16s rrna gene sequence and pcr-rflp analyses were used to examine ba ...201120228207
diversity of rhizobial bacteria isolated from nodules of the gypsophyte ononis tridentata l. growing in spanish soils.the aim of this work is to describe the diversity and phylogeny of rhizobial bacteria associated to nodules of ononis tridentata l. in different geographical regions of spain. twenty-two bacterial isolates were characterized using several molecular techniques (16s amplified ribosomal deoxyribonucleic acid restriction analysis, fingerprinting, and sequencing) and phylogenies were inferred from their 16s and nodc gene sequences. phylogenetically, the isolates grouped with the genera rhizobium, mes ...200818060448
emended description of the genus phyllobacterium and description of four novel species associated with plant roots: phyllobacterium bourgognense sp. nov., phyllobacterium ifriqiyense sp. nov., phyllobacterium leguminum sp. nov. and phyllobacterium brassicacearum sp. nov.gram-negative bacteria were isolated from the rhizoplane of brassica napus in france and from root nodules of argyrolobium uniflorum, astragalus algerianus and lathyrus numidicus growing in the infra-arid zone of southern tunisia. based on phylogenetic analysis of the 16s rrna gene sequences, the seven isolates belong to the alphaproteobacteria and are related to phyllobacterium myrsinacearum strains. the isolates formed three clusters; clusters a and c consist of tunisian strains, whereas clust ...200616585703
rapid and specific identification of four agrobacterium species and biovars using multiplex pcr.on the basis of 23s rrna gene sequences, 1 universal forward and 4 taxon (species/biovar)-specific reverse primers were designed for multiplex pcr to aid in identification and differentiation of agrobacterium rubi, agrobacterium vitis and agrobacterium biovars 1 and 2. in reactions with dna of 119 bacterial strains belonging to: agrobacterium, allorhizobium, mesorhizobium, rhizobium, sinorhizobium and phyllobacterium, as well as phytopathogenic bacteria representing various genera, the primers d ...200616343837
phyllobacterium trifolii sp. nov., nodulating trifolium and lupinus in spanish soils.bacterial strain petp02(t) was isolated from nodules of trifolium pratense growing in a spanish soil. phylogenetic analysis of the 16s rrna gene sequence showed that this strain represents a member of the genus phyllobacterium. however, divergence found with the 16s rrna gene sequence of the single recognized species of this genus, phyllobacterium myrsinacearum, indicated that strain petp02(t) belongs to a different species. the results of dna-dna hybridization, phenotypic tests and fatty acid a ...200516166699
nitrate-dependent control of root architecture and n nutrition are altered by a plant growth-promoting phyllobacterium sp.both root architecture and plant n nutrition are altered by inoculation with the plant growth-promoting rhizobacteria (pgpr) phyllobacterium strain stm196. it is known that no3- and n metabolites can act as regulatory signals on root development and n transporters. in this study, we investigate the possible interrelated effects on root development and n transport. we show that the inhibition of arabidopsis lateral root growth by high external no3- is overridden by phyllobacterium inoculation. ho ...200616160849
phyllobacterium catacumbae sp. nov., a member of the order 'rhizobiales' isolated from roman catacombs.two strains were isolated from tuff, a volcanic rock that forms the walls of the roman catacombs of saint callixtus in rome, italy. a polyphasic approach using nutritional and physiological tests, reactions to antibiotics, fatty acid profiles, dna base ratios, dna-dna reassociation and 16s rrna gene sequence comparisons showed that the two isolates belong to a novel species within the genus phyllobacterium. the species phyllobacterium catacumbae sp. nov. is proposed. the type strain is csc19(t) ...200516014469
a highly selectable and highly transferable ti plasmid to study conjugal host range and ti plasmid dissemination in complex ecosystems.a conjugal donor system, st2, was constructed to study the conjugal dissemination of a ti plasmid to wild-type recipient bacteria in vitro and in situ. the system consisted of a polyauxotrophic derivative of c58 harboring a hyperconjugative and highly selectable ti plasmid, pstiegk, which was constructed by inserting a multiple antibiotic resistance cassette in the tram- mcpa region of ptic58delta accr. st2 transfers pstiegk constitutively at frequencies up to 10(-1) to plasmidless agrobacterium ...200415164241
phylogenies of atpd and reca support the small subunit rrna-based classification of rhizobia.the current classification of the rhizobia (root-nodule symbionts) assigns them to six genera. it is strongly influenced by the small subunit (16s, ssu) rrna molecular phylogeny, but such single-gene phylogenies may not reflect the evolution of the genome as a whole. to test this, parts of the atpd and reca genes have been sequenced for 25 type strains within the alpha-proteobacteria, representing species in rhizobium, sinorhizobium, mesorhizobium, bradyrhizobium, azorhizobium, agrobacterium, ph ...200111760945
identity and potential functions of heterotrophic bacterial isolates from a continuous-upflow fixed-bed reactor for denitrification of drinking water with bacterial polyester as source of carbon and electron donor.a collection of 186 heterotrophic bacteria, isolated directly from a continuous-upflow fixed-bed reactor for the denitrification of drinking water, in which poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (phbv) granules acted as biofilm carrier, carbon source and electron donor, was studied with regard to taxonomic affiliation and degradation and denitrification characteristics. two granule samples were taken from a fully operating reactor for enumeration and isolation of heterotrophic bacteria. o ...200111518336
an alternative interpretation of nanobacteria-induced biomineralization.the reported isolation of nanobacteria from human kidney stones raises the intriguing possibility that these microorganisms are etiological agents of pathological extraskeletal calcification [kajander, e. o. & ciftçioglu, n. (1998) proc. natl. acad. sci. usa 95, 8274-8279]. nanobacteria were previously isolated from fbs after prolonged incubation in dmem. these bacteria initiated biomineralization of the culture medium and were identified in calcified particles and biofilms by nucleic acid stain ...200011027350
Biogeography of symbiotic and other endophytic bacteria isolated from medicinal Glycyrrhiza species in China.A total of 159 endophytic bacteria were isolated from surface-sterilized root nodules of wild perennial Glycyrrhiza legumes growing on 40 sites in central and northwestern China. Amplified fragment length polymorphism (AFLP) genomic fingerprinting and sequencing of partial 16S rRNA genes revealed that the collection mainly consisted of Mesorhizobium, Rhizobium, Sinorhizobium, Agrobacterium and Paenibacillus species. Based on symbiotic properties with the legume hosts Glycyrrhiza uralensis and Gl ...201122066910
lutibaculum baratangense gen. nov., sp. nov., a novel proteobacterium isolated from a mud volcano, andamans, india.a novel gram-negative, oval to rod shaped, motile bacterium, strain amv1t, was isolated from a soil sample collected from a mud volcano, baratang island, andamans, india. the fatty acids were dominated by unsaturated fatty acids (82.6 %), with a high presence of c18:1 ω7c (78.6 %). strain amv1t contained ubiquinone (q9) and menaquinone (mk-4 and mk-8) as the respiratory quinones. the polar lipids consisted of four unknown aminolipids, two unknown glycoaminolipids and two unknown phospholipids. t ...201021148673
diverse rhizobia associated with sophora alopecuroides grown in different regions of loess plateau in china.a total of seventy-five symbiotic bacterial strains isolated from root nodules of wild sophora alopecuroides grown in different regions of china's loess plateau were characterized. based on the combined rflp patterns, thirty-five genotypes were defined among the rhizobia and they were classified into nine genomic species, including mesorhizobium alhagi and m. gobiense as the main groups, as well as agrobacterium tumefaciens, m. amorphae, phyllobacterium trifolii, rhizobium giardinii, r. indigofe ...201020965680
the auxin-signaling pathway is required for the lateral root response of arabidopsis to the rhizobacterium phyllobacterium brassicacearum.plant root development is highly responsive both to changes in nitrate availability and beneficial microorganisms in the rhizosphere. we previously showed that phyllobacterium brassicacearum stm196, a plant growth-promoting rhizobacteria strain isolated from rapeseed roots, alleviates the inhibition exerted by high nitrate supply on lateral root growth. since soil-borne bacteria can produce iaa and since this plant hormone may be implicated in the high nitrate-dependent control of lateral root d ...201020844890
diverse bacteria isolated from root nodules of wild vicia species grown in temperate region of the present study, a total of 154 bacterial strains isolated from nodules of eighteen vicia species mainly grown in the temperate chinese provinces were characterized by ardra, its pcr-rflp, box-pcr, sequencing of 16s rdna, nodc, nifh, atpd and glnii, and nodulation tests. the results demonstrated that most of the r. leguminosarum strains were effective microsymbionts of the wild vicia species, while genomic species related to rhizobium gallicum, mesorhizobium huakuii, ensifer meliloti and br ...200818704366
Bacterial communities in urban aerosols collected with wetted-wall cyclonic samplers and seasonal fluctuations of live and culturable airborne bacteria.Airborne transmission of bacterial pathogens from point sources (e.g., ranches, dairy waste treatment facilities) to areas of food production (farms) has been suspected. Determining the incidence, transport and viability of extremely low levels of pathogens require collection of high volumes of air and characterization of live bacteria from aerosols. We monitored the numbers of culturable bacteria in urban aerosols on 21 separate days during a 9 month period using high volume cyclonic samplers a ...201122193549
characterization of heterotrophic prokaryote subgroups in the sfax coastal solar salterns by combining flow cytometry cell sorting and phylogenetic, we combined flow cytometry (fcm) and phylogenetic analyses after cell sorting to characterize the dominant groups of the prokaryotic assemblages inhabiting two ponds of increasing salinity: a crystallizer pond (ts) with a salinity of 390 g/l, and the non-crystallizer pond (m1) with a salinity of 200 g/l retrieved from the solar saltern of sfax in tunisia. as expected, fcm analysis enabled the resolution of high nucleic acid content (hna) and low nucleic acid content (lna) prokaryotes. next ...201121424516
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