| phylogenetic analysis of the family rhizobiaceae and related bacteria by sequencing of 16s rrna gene using pcr and dna sequencer. | the 16s rrna gene sequences of 19 strains covering 97% of the molecules were determined for the members of the family rhizobiaceae and related bacteria by pcr and dna sequencer. the three biovars of agrobacterium were located separately, whereas agrobacterium rubi clustered with a. tumefaciens. phylogenetic locations for the species of the genera rhizobium, sinorhizobium, agrobacterium, phylobacterium, mycoplana (m. dimorpha), ochrobactrum, brucella and rochalimaea (a rickettsia) were intermingl ... | 1993 | 7682191 |
| proposal for rejection of agrobacterium tumefaciens and revised descriptions for the genus agrobacterium and for agrobacterium radiobacter and agrobacterium rhizogenes. | the 16s rrna sequences of seven representative agrobacterium strains, eight representative rhizobium strains, and the type strains of azorhizobium caulinodans and bradyrhizobium japonicum were determined. these strains included the type strains of agrobacterium tumefaciens, agrobacterium rhizogenes, agrobacterium radiobacter, agrobacterium vitis, agrobacterium rubi, rhizobium fredii, rhizobium galegae, rhizobium huakuii, rhizobium leguminosarum, rhizobium loti, rhizobium meliloti, and rhizobium ... | 1993 | 8240952 |
| unconventional genomic organization in the alpha subgroup of the proteobacteria. | pulsed-field gel electrophoresis was used to analyze the genomic organization of 16 bacteria belonging or related to the family rhizobiaceae of the alpha subgroup of the class proteobacteria. the number and sizes of replicons were determined by separating nondigested dna. hybridization of an rrn gene probe was used to distinguish between chromosomes and plasmids. members of the genus agrobacterium all possess two chromosomes, and each biovar has a specific genome size. as previously demonstrated ... | 1998 | 9573163 |
| novel tellurite-amended media and specific chromosomal and ti plasmid probes for direct analysis of soil populations of agrobacterium biovars 1 and 2. | ecology and biodiversity studies of agrobacterium spp. require tools such as selective media and dna probes. tellurite was tested as a selective agent and a supplement of previously described media for agrobacteria. the known biodiversity within the genus was taken into account when the selectivity of k(2)teo(3) was analyzed and its potential for isolating agrobacterium spp. directly from soil was evaluated. a k(2)teo(3) concentration of 60 ppm was found to favor the growth of agrobacteria and r ... | 2001 | 11133429 |
| mesorhizobium septentrionale sp. nov. and mesorhizobium temperatum sp. nov., isolated from astragalus adsurgens growing in the northern regions of china. | ninety-five rhizobial strains isolated from astragalus adsurgens growing in the northern regions of china were classified into three main groups, candidate species i, ii and iii, based on a polyphasic approach. comparative analysis of full-length 16s rrna gene sequences of representative strains showed that candidate species i and ii were mesorhizobium, while candidate species iii, which consisted of non-nodulating strains, was closely related to agrobacterium tumefaciens. the phylogenetic relat ... | 2004 | 15545425 |
| phylogenetic analysis of the genera bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium on the basis of 16s rrna gene and internally transcribed spacer region sequences. | a total of 128 strains was isolated from more than 23 legume hosts in korea. phylogenetic relationships between these korean isolates and reference strains of the genera bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium were analysed using their 16s rrna gene and internally transcribed spacer (its) region sequences. among the bradyrhizobium strains, dendrograms based on both the 16s rrna gene and its region sequences produced two main groups. the its tree yielded at least two new cluste ... | 2005 | 15653885 |
| rhizobial 16s rrna and dnak genes: mosaicism and the uncertain phylogenetic placement of rhizobium galegae. | the phylogenetic relatedness among 12 agriculturally important species in the order rhizobiales was estimated by comparative 16s rrna and dnak sequence analyses. two groups of related species were identified by neighbor-joining and maximum-parsimony analysis. one group consisted of mesorhizobium loti and mesorhizobium ciceri, and the other group consisted of agrobacterium rhizogenes, rhizobium tropici, rhizobium etli, and rhizobium leguminosarum. although bootstrap support for the placement of t ... | 2005 | 15746335 |
| agrobacterium rubi(t) dsm 6772 produces a lipophilic polysaccharide capsule whose degree of acetylation is growth modulated. | the structure of the capsular polysaccharide produced from the type strain of agrobacterium rubi dsm 6772 is demonstrated by means of chemical and spectroscopical methodologies. it is constituted from the quite rare monosaccharide 6-deoxy-l-talose, involved in alternating alpha-(1 --> 2) and alpha-(1 --> 3) linkages. this simple backbone is further complicated from the occurrence of o-acetyl substituents located always at o-2 of the o-3 substituted 6-deoxy-talose. this decoration is not stoichio ... | 2007 | 17284069 |
| rhizobium rubi(t): a gram-negative phytopathogenic bacterium expressing the lewis b epitope on the outer core of its lipooligosaccharide fraction. | the structure of the core oligosaccharide from the phytopathogenic bacterium rhizobium rubi was deduced by combining information from complementary chemical approaches (alkaline and acid hydrolysis), similar to the "overlap peptide" strategy. this structure is new and it contains two main oligosaccharide backbones that differ in the substitution degree of the external kdo unit. the relevant feature shared by both oligosaccharides is the presence of a tetrasaccharide motif that is similar to the ... | 2008 | 18574826 |
| rhizobium pusense sp. nov., isolated from rhizosphere of chickpea (cicer arietinum l). | a bacterial strain nrcpb10t was isolated from rhizosphere soil of chickpea (cicer arietinum l), in pusa, new delhi, india.16s rrna gene sequence of the strain nrcpb10t showed the highest similarity 98.9% with rhizobium radiobacter icmp 5785t followed by rhizobium larrymoorei icmp 15237t (97.7%) and rhizobium rubi lmg 17935t (97.4%). phylogenetic analysis of strain nrcpb10t using housekeeping genes reca and atpd revealed their distinct position differing from other recognized rhizobium species. d ... | 2010 | 21148676 |
| rhizobium skierniewicense sp. nov. isolated from tumors on chrysanthemum and prunus in poland. | three gram-negative, rod-shaped, non-spore-forming bacteria were isolated from galls on chrysanthemum (ch11(t), ch12) and on prunus cerasifera var. divaricata (al 9.3). all three isolates are able to cause crown gall on different plant species. on the basis of 16s rrna gene sequence similarity, the strains belong to the genus rhizobium and their closest relatives are rhizobium rubi (99.6%), rhizobium radiobacter (98.7%) and rhizobium larrymoorei (98.1%). phylogenetic analysis of the novel strain ... | 2011 | 21642489 |
| rapid and specific identification of four agrobacterium species and biovars using multiplex pcr. | on the basis of 23s rrna gene sequences, 1 universal forward and 4 taxon (species/biovar)-specific reverse primers were designed for multiplex pcr to aid in identification and differentiation of agrobacterium rubi, agrobacterium vitis and agrobacterium biovars 1 and 2. in reactions with dna of 119 bacterial strains belonging to: agrobacterium, allorhizobium, mesorhizobium, rhizobium, sinorhizobium and phyllobacterium, as well as phytopathogenic bacteria representing various genera, the primers d ... | 2006 | 16343837 |
| rhizobium daejeonense sp. nov. isolated from a cyanide treatment bioreactor. | a polyphasic study was carried out to determine the taxonomic position of two aerobic, cyanide-degrading bacterial strains, designated l61t and l22, which had been isolated from a bioreactor for the treatment of nickel-complexed cyanide. the two isolates exhibited almost identical taxonomic characteristics. phylogenetic analysis inferred from comparative 16s rrna gene sequences indicated that the isolates fall in a sublineage of the genus rhizobium comprising the type strains of rhizobium giardi ... | 2005 | 16280524 |
| nucleoprotein changes in plant tumor growth. | tumor cell transformation and growth were studied in a plant neoplasm, crown gall of bean, induced by agrobacterium rubi. ribose nucleic acid (rna), deoxyribose nucleic acid (dna), histone, and total protein were estimated by microphotometry of nuclei, nucleoli, and cytoplasm in stained tissue sections. transformation of normal cells to tumor cells was accompanied by marked increases in ribonucleoprotein content of affected tissues, reaching a maximum 2 to 3 days after inoculation with virulent ... | 1959 | 13673042 |
| a revision of rhizobium frank 1889, with an emended description of the genus, and the inclusion of all species of agrobacterium conn 1942 and allorhizobium undicola de lajudie et al. 1998 as new combinations: rhizobium radiobacter, r. rhizogenes, r. rubi, r. undicola and r. vitis. | rhizobium, agrobacterium and allorhizobium are genera within the bacterial family rhizobiaceae, together with sinorhizobium. the species of agrobacterium, agrobacterium tumefaciens (syn. agrobacterium radiobacter), agrobacterium rhizogenes, agrobacterium rubi and agrobacterium vitis, together with allorhizobium undicola, form a monophyletic group with all rhizobium species, based on comparative 16s rdna analyses. agrobacterium is an artificial genus comprising plant-pathogenic species. the monop ... | 2001 | 11211278 |
| identification of agrobacterium strains by pcr-rflp analysis of pti and chromosomal regions. | chromosomes and ti plasmids of 41 agrobacterium strains, belonging to biovars 1, 2, 3, and agrobacterium rubi species were characterized by the restriction fragment length polymorphism of pcr-amplified dnas. profiles that were obtained by the analysis of the amplified 16s rdna confirmed the grouping of the strains according to their species. higher polymorphism was detected in the intergenic spacer between the 16s rdna and 23s rdna genes, allowing efficient discrimination of strains. identificat ... | 1994 | 7911654 |
| a new agrobacterium strain isolated from aerial tumors on ficus benjamina l. | crown gall tumors, collected from branches of 1-year-old weeping fig (ficus benjamina l.) trees, yielded both tumorigenic and nonpathogenic agrobacteria. on the basis of classical diagnostic tests, the nonpathogenic strains were identified as agrobacterium tumefaciens, whereas the tumorigenic strains could not be assigned to any of the known terrestrial agrobacterium spp. the tumorigenic strains also differed from other members of the genus by producing more acid from mannitol. according to clus ... | 1995 | 7887626 |
| tracking composition of microbial communities for simultaneous nitrification and denitrification in polyurethane foam. | the process of simultaneous nitrification and denitrification (snd) of immobilized microorganisms in polyurethane form is discussed. the effect of different positions within the polyurethane carrier on microbial community response for the snd process is investigated by a combination of denaturing gradient gel electrophoresis profiles of the 16s rrna gene v3 region and scanning electron microscopy. results show that polyurethane, which consists of a unique porous structure, is an ideal platform f ... | 2014 | 24804651 |
| [agrobacterium rubi strains from blueberry plants are highly diverse]. | the diversity of a collection of agrobacterium rubi strains isolated from blueberries from different regions of argentina was studied by conventional microbiological tests and molecular techniques. results from biochemical and physiological reactions, as well as from rep-pcr and rflp analysis of pcr-amplified 23s rdna showed high phenotypic and genotypic intraspecific variation. | 2016 | 25444133 |
| rapid and efficient identification of agrobacterium species by reca allele analysis: agrobacterium reca diversity. | the analysis of housekeeping reca gene sequences from 138 strains from 13 species or genomic species of agrobacterium, nine being biovar 1 genomospecies, and the others agrobacterium larrymoorei, agrobacterium rubi, agrobacterium sp. ncppb 1650, and agrobacterium vitis and one "former" agrobacterium species, rhizobium rhizogenes, led to the identification of 50 different reca alleles and to a clear delineation of the 14 species or genomospecies entirely consistent with that obtained by amplified ... | 2010 | 20521039 |
| a novel capsular polysaccharide from rhizobium rubi strain dsm 30149. | rhizobium rubi, strain dsm 30149, is a gram negative phytopathogenic bacterium which produces a linear polysaccharide with the following repeating unit: this new structure was determined by spectroscopical and chemical methods. it presents similar lipophilic features reported for another strain of r. rubi. these contrast with features already known for capsular polysaccharide species from symbiontic members of the rhizobiaceae family, namely highly anionic polymers. | 2008 | 18468587 |