proposal of burkholderia gen. nov. and transfer of seven species of the genus pseudomonas homology group ii to the new genus, with the type species burkholderia cepacia (palleroni and holmes 1981) comb. nov.based on the 16s rrna sequences, dna-dna homology values, cellular lipid and fatty acid composition, and phenotypic characteristics, a new genus burkholderia is proposed for the rna homology group ii of genus pseudomonas. seven species in this group were transferred to the new genus. thus seven new combinations, burkholderia cepacia (palleroni and holmes 1981), burkholderia mallei (zopf 1885), burkholderia pseudomallei (whitmore 1913), burkholderia caryophylli (burkholder 1942), burkholderia gla ...19921283774
screening test of the biodegradative capability of a new strain of pseudomonas gladioli (bsu 45124) on some xenobiotic organics. 19921392282
somatic antigens of pseudomonads: structure of the o-specific polysaccharide chain of pseudomonas gladioli pv. alliicola 8494 (serogroup x) lipopolysaccharide. 19911720349
[electron microscopic observations on pseudomonas cocovenenans].a strain of food-poisoning bacterium has been isolated by jin jiexiang (1963) in china from the fermented cornflour that has gone bad. these pathogenic microorganism has been identified and named pseudomonas by zhao naixin in 1988, which is the same species as p. cocovenenans. the characteristics of them were conformed to these of the species p. cepacia of section 2 of the genus pseudomonas. in view of the fact that the fine structures of the above mentioned three strains of pseudomonas have not ...19902270688
pneumonia caused by a newly recognized pseudomonad in a child with chronic granulomatous disease.a pseudomonad was isolated from the pleural fluid and pulmonary decortication tissue of a 5-year-old child with chronic granulomatous disease. although the isolate was phenotypically similar to pseudomonas cepacia, its biochemical profile was more similar to that of pseudomonas pickettii biovar 2. its slow growth rate, ability to hydrolyze urea rapidly, and lateral and polar flagellar pattern were suggestive of oligella ureolytica (formerly cdc group ive). the cellular fatty acid composition was ...19902380349
inhibition of bongkrek acid and toxoflavin production in tempe bongkrek containing pseudomonas cocovenenans.tempe bongkrek was prepared from partially defatted coconut by fermentation with the mould rhizopus oligosporus. the addition of 2% sodium chloride decreased but did not inhibit formation of bongkrek acid by pseudomonas cocovenenans strain itb in either coconut culture medium (ccm) or tempe bongkrek, while 2% sodium chloride or acidification of ccm to ph 4.5 with acetic acid inhibited formation of toxoflavin. toxin formation by three strains of ps. cocovenenans in tempe bongkrek was inhibited by ...19902391293
fermented corn flour poisoning in rural areas of china. iii. isolation and identification of main toxin produced by causal microorganisms.flavotoxin a was isolated from pseudomonas cocovenenans subsp. farinofermentans culture in semisolid potato-dextrose-agar medium, which was isolated from fermented corn meal that had caused food poisoning outbreaks in china. the isolation, purification, and chemical structure of this toxin were studied. the nmr spectra, the uv spectra, and molar extinction coefficients, and the mass spectra of flavotoxin a are in good agreement with those reported for bongkrekic acid. therefore, flavotoxin a and ...19892590494
recovery of pseudomonas gladioli from respiratory tract specimens of patients with cystic fibrosis.pseudomonas gladioli was isolated from 11 patients with cystic fibrosis. it resembled pseudomonas cepacia on the selective and differential medium ofpbl, producing yellow colonies after 48 to 72 h of incubation. isolates were characterized biochemically, by dna hybridization, and by cellular fatty acid analysis. a review of the clinical status of selected patients colonized by p. gladioli did not reveal any apparent association of this organism with infectious complications of cystic fibrosis. t ...19892915020
mm 42842, a new member of the monobactam family produced by pseudomonas cocovenenans. i. identification of the producing organism.a bacterial soil isolate designated 326-32b produces a new member of the monobactam series of antibiotics, mm 42842, and the bulgecins. identification studies show isolate 326-32b to be a strain of pseudomonas cocoveneans which is a species previously noted for the production of toxoflavin. a description of p. cocovenenans does not appear to have been previously published and the identify of strain 326-32b was established by means of a direct comparison with the deposited organism p. cocovenenan ...19883346181
on the biosynthesis of toxoflavin, an azapteridine antibiotic produced by pseudomonas cocovenenans. 19665905124
multi-resistance isolates possessing characteristics of both burkholderia (pseudomonas) cepacia and burkholderia gladioli from patients with cystic fibrosis.multi-resistant strains from three uk centres, previously identified as burkholderia (formerly pseudomonas) cepacia, and associated with morbidity, mortality and transmission among patients with cystic fibrosis have been further characterised. biochemical tests and fatty acid analyses indicate these strains to possess some characteristics atypical of b. cepacia but bearing close resemblance to burkholderia gladioli, an organism previously regarded solely as a plant pathogen and a hindrance to th ...19947530242
oligonucleotide primers designed to differentiate pathogenic pseudomonads on the basis of the sequencing of genes coding for 16s-23s rrna internal transcribed spacers.universal primers targeting conserved sequences flanking the 3' end of the 16s and the 5' end of the 23s rrna genes (rdnas) were used to amplify the 16s-23s rdna internal transcribed spacers (its) from eight species of pseudomonads which have been associated with human infections. amplicons from reference strains of pseudomonas aeruginosa, pseudomonas cepacia, pseudomonas gladioli, pseudomonas mallei, pseudomonas mendocina, pseudomonas pickettii, pseudomonas pseudomallei, and xanthomonas maltoph ...19957583922
biological activity of burkholderia (pseudomonas) cepacia lipopolysaccharide.burkholderia cepacia has emerged as an important multiresistant pathogen in cystic fibrosis (cf), associated in 20% of colonised patients with a rapid and fatal decline in lung function. although knowledge of b. cepacia epidemiology has improved, the mechanisms involved in pathogenesis remain obscure. in this study, b. cepacia lipopolysaccharide (lps) was assessed for endotoxic potential and the capacity to induce tumour necrosis factor (tnf). lps preparations from clinical and environmental iso ...19957640678
severe burkholderia (pseudomonas) gladioli infection in chronic granulomatous disease: report of two successfully treated cases.chronic granulomatous disease (cgd) is characterized by a defect in phagocytic cells that leads to recurrent superficial and deep pyogenic infections. burkholderia (pseudomonas) gladioli is a gram-negative bacillus in the pseudomallei group of pseudomonads that is known primarily as a plant pathogen. we report two cases of pneumonia, one accompanied by septicemia, caused by b. gladioli in patients with cgd and their successful treatment with antibiotics. we believe these represent the first repo ...19958589158
phylogenetic evidence for the transfer of pseudomonas cocovenenans (van damme et al. 1960) to the genus burkholderia as burkholderia cocovenenans (van damme et al. 1960) comb. nov.pseudomonas cocovenenans, the producer of bongkrekic acid and toxoflavin, has been described previously by us (n.-x. zhao, m.-s. ma, y.-p. zhang, and d.-c. xu, int. j. syst. bacteriol. 40:452-455, 1990) in terms of more than 180 phenotypic traits, g+c content, and dna relatedness. the bacterium conformed to section ii of the genus pseudomonas. here, its partial 16s rrna gene sequence was compared with those of burkholderia spp., and the results verified that p. cocovenenans is a burkholderia spe ...19958590691
empyema and bloodstream infection caused by burkholderia gladioli in a patient with cystic fibrosis after lung transplantation. 19968823866
chronic granulomatous disease presenting as severe sepsis due to burkholderia gladioli. 19968842296
bioassay methods for the detection of antifungal activity by pseudomonas antimicrobica against the grey mould pathogen botrytis cinerea.antagonism against the grey mould pathogen botrytis cinerea by pseudomonas antimicrobica was demonstrated in vitro and in vivo. cell-free filtrates showed activity against b. cinerea growing on potato dextrose agar (pda) in a media-dependent manner with the most distinct antagonism being produced in czapek dox broth (cdb). cell-free filtrates of cdb-grown cultures also significantly reduced conidial germination of b. cinerea. an assay based on the inhibition of conidial germination was compared ...19968939031
isolation from clinical sources of burkholderia cepacia possessing characteristics of burkholderia gladioli.further characterization of 32 clinical isolates originally identified as burkholderia cepacia by biochemical and fatty acid profiling revealed the presence of 12 strains bearing partial resemblance to the closely related species burkholderia gladioli. these strains were highly resistant to a wide range of antibiotics including ticarcillin (with or without clavulanic acid), cefsulodin, imipenem, the aminoglycosides, colistin and fosfomycin. they typically produced a red-brown pigment and possess ...19979069537
structural studies of the o-specific side-chain of lipopolysaccharide from burkholderia gladioli pv. gladioli strain ncppb 1891.a polymeric fraction (the o-antigenic side-chain) has been isolated from the lipopolysaccharide of burkholderia gladioli pv. gladioli strain ncppb 1891 after mild acid hydrolysis. the components of the polymer and their molar proportions were l-rha (1), d-gal (1), d-man (1), and o-acetyl (1). by means of chemical degradations and nmr studies, the repeating unit of the polymer was shown to be a linear trisaccharide of the structure shown. [formula: see text]19979352638
four additional cases of burkholderia gladioli infection with microbiological correlates and review.burkholderia gladioli has only recently been reported to be a human pathogen. four cases of b. gladioli infection (including bacteremia, pneumonia, and cervical adenitis) in two adults and two young children are reported. three of these four patients were severely immunocompromised. commercial systems were frequently unable to identify this bacterium correctly. antimicrobial susceptibility patterns indicated that b. gladioli strains were susceptible to the quinolones, aminoglycosides, and imipen ...19979356798
infections in patients with cystic fibrosis following lung transplantation.there is controversy over whether colonization with drug-resistant organisms is a contraindication to lung transplantation.19979377954
bacteremia due to burkholderia gladioli: case report. 19979402407
burkholderia pseudomallei infection in a puerto rican patient with chronic granulomatous disease: case report and review of occurrences in the americas.burkholderia species, notably burkholderia cepacia and burkholderia gladioli, are important pathogens in patients with chronic granulomatous disease (cgd). burkholderia pseudomallei, the causative agent of melioidosis, is endemic in southeast asia and northern australia but is a rare pathogen in other parts of the world. we describe the occurrence of b. pseudomallei infection in a puerto rican patient with cgd. this is one of only a small number of documented cases of melioidosis autochthonous t ...19989564470
development and characterization of a murine monoclonal antibody reactive with a 64 kda somatic antigen of burkholderia cepacia.monoclonal antibodies (mabs) to burkholderia cepacia were produced from mice immunized with inactivated whole-cell antigen. for screening of resulting mabs an enzyme-linked immunosorbent assay (elisa) was used. a stable hybridoma cell line (bc-2) producing specific antibodies to a 64 kda somatic antigen from b. cepacia was established. in elisa and immunoblotting analysis the mab bc-2 recognized all tested strains of b. cepacia whereas no cross-reaction with 32 pseudomonas aeruginosa strains was ...19989627054
detection of a new enzyme for stereoselective hydrolysis of linalyl acetate using simple plate assays for the characterization of cloned esterases from burkholderia gladioli.plate assays were developed for the identification of specific hydrolytic activities of esterases from burkholderia gladioli, cloned and expressed in escherichia coli. clones showing different substrate specificities were identified by fluorescence or azo-dye formation caused by the released alcohol moiety of the hydrolyzed substrates, or by colour change of ph indicators mediated by decreased ph. the use of 1-hydroxypyrene-3,6,8-trisulfonic acid (hpts-) esters and linalyl acetate for these assa ...19989684341
discrimination of burkholderia gladioli from other burkholderia species detectable in cystic fibrosis patients by pcr.a procedure for molecular identification of burkholderia gladioli is described. specific 16s and 23s rrna gene signature sequences were defined as primers for pcr. the method allows rapid and specific discrimination of b. gladioli from related species (b. cepacia, b. multivorans, b. vietnamiensis, b. mallei, b. pseudomallei, ralstonia pickettii, and r. eutropha) and should contribute to the clarification of its role as a human pathogen, e.g., in cystic fibrosis.19989705429
significance of airway colonization by burkholderia gladioli in lung transplant candidates. 19989726771
ratios of carbon isotopes in microbial lipids as an indicator of substrate usage.the occurrence and abundance of microbial fatty acids have been used for the identification of microorganisms in microbial communities. however, these fatty acids can also be used as indicators of substrate usage. for this, a systematic investigation of the discrimination of the stable carbon isotopes by different microorganisms is necessary. we grew 11 strains representing major bacterial and fungal species with four different isotopically defined carbon sources and determined the isotope ratio ...19989797266
nosocomial acquisition of burkholderia gladioli in patients with cystic fibrosis. 199910390428
the effect of lipids on bongkrekic (bongkrek) acid toxin production by burkholderia cocovenenans in coconut media.tempe bongkrek is an indonesian food made by fermentation of coconut presscake or coconut milk residue rhizopus oligosporus. consumption of tempe bongkrek is associated with a food-borne human intoxication and significant numbers of deaths annually. the bacterium burkholderia cocovenenans, which is the causative organism, produces two toxins, toxoflavin and bongkrekic acid (also commonly referred to as bongkrek acid). the reasons why these poisonings occur only in a very limited number of foods ...199910435074
evaluation of three oligonucleotide primer sets in pcr for the identification of burkholderia cepacia and their differentiation from burkholderia evaluate three oligonucleotide primer pairs--two specific for 16s and 23s rrna sequences of burkholderia cepacia, and the third specific for internal transcribed spacer region of 16s-23s sequences of b gladioli--for the identification and differentiation of reference and clinical strains of these and other species.199910450174
a simplified subtractive hybridization protocol used to isolate dna sequences specific to xylella fastidiosa.a simplified protocol of subtractive hybridization based on the technique of l. m. kunkel, a. p. monaco, w. middlesworth, h. d. ochs & s. a. latt (1985, proc natl acad sci usa, 82, 4778-4782) was used to obtain dna sequences specific to xylella fastidiosa isolated from diseased citrus plants. as a driver, dna extracted from bacteria showing different degrees of relatedness was used: xy. fastidiosa 788 isolated from another host (plum), xanthomonas campestris pv. campestris and burkholderia gladi ...199910463163
development of rrna-based pcr assays for identification of burkholderia cepacia complex isolates recovered from cystic fibrosis patients.pcr assays targeting rrna genes were developed to identify species (genomovars) within the burkholderia cepacia complex. each assay was tested with 177 bacterial isolates that also underwent taxonomic analysis by whole-cell protein profile. these isolates were from clinical and environmental sources and included 107 b. cepacia complex strains, 23 burkholderia gladioli strains, 20 ralstonia pickettii strains, 10 pseudomonas aeruginosa strains, 8 stenotrophomonas maltophilia strains, and 9 isolate ...199910488171
species-specific pcr as a tool for the identification of burkholderia gladioli.burkholderia gladioli colonizes the respiratory tracts of patients with cystic fibrosis and chronic granulomatous disease. however, due to the high degree of phenotypic similarity between this species and closely related species in the burkholderia cepacia complex, accurate identification is difficult. incorrect identification of these species may have serious repercussions for the management of patients with cystic fibrosis. to develop an accurate procedure for the identification of b. gladioli ...200010618102
[relationship between the toxigenicity and ribotype distribution of pseudomonas cocovenenans subsp. farinofermentans].by rdna fingerprinting technique, ribotype discrimination analysis was conducted for exploring the relationship between the toxigenicity and ribotype distribution of 42 strains of pseudomonas cocovenenans subsp. farinofermentans. the results showed that 73.8 percent of the strains were toxigenic, which were distributed within the most ribotype and isolated from either food poisoning samples or normal food samples. the other strains were not toxigenic; which were isolated from normal food samples ...199810682622
distinguishing species of the burkholderia cepacia complex and burkholderia gladioli by automated ribotyping.several species belonging to the genus burkholderia are clinically relevant, opportunistic pathogens that inhabit major environmental reservoirs. consequently, the availability of means for adequate identification and epidemiological characterization of individual environmental or clinical isolates is mandatory. in the present communication we describe the use of the riboprinter microbial characterization system (qualicon, warwick, united kingdom) for automated ribotyping of 104 strains of burkh ...200010790116
utility of commercial systems for identification of burkholderia cepacia complex from cystic fibrosis sputum culture.performances of several commercial test systems were reviewed to determine their relative levels of accuracy in identifying burkholderia cepacia complex isolates recovered from cystic fibrosis sputum culture. positive predictive values ranged from 71 to 98%; negative predictive values ranged from 50 to 82%. all systems misidentified b. cepacia complex. the species most frequently misidentified as b. cepacia was burkholderia gladioli. these data support the results of previous studies that recomm ...200010921992
molecular cloning and characterization of a chitosanase from the chitosanolytic bacterium burkholderia gladioli strain chb101.a chitosanase was purified from the culture fluid of the chitino- and chitosanolytic bacterium burkholderia gladioli strain chb101. the purified enzyme (chitosanase a) had a molecular mass of 28 kda, and catalyzed the endo-type cleavage of chitosans having a low degree of acetylation (0-30%). the enzyme hydrolyzed glucosamine oligomers larger than a pentamer, but did not exhibit any activity toward n-acetylglucosamine oligomers and colloidal chitin. the gene coding for chitosanase a (csna) was i ...200011030572
cloning and characterization of estc from burkholderia gladioli, a novel-type esterase related to plant screening a genomic library of burkholderia gladioli (formerly pseudomonas marginata) for clones exhibiting esterolytic activity, the gene for a novel-type esterase (estc) showing significant homology to plant enzymes could be isolated. high homology was found to two hydroxynitrile lyases originating from hevea brasiliensis (tropical rubber tree) and manihot esculenta (cassava), and to two proteins from oryza sativa (rice) that are specifically induced upon infection by pseudomonas syringae p ...200011152069
pseudomonas antimicrobica attafuah and bradbury 1990 is a junior synonym of burkholderia gladioli (severini 1913) yabuuchi et al. 1993.comparison of the 16s rdna sequence of pseudomonas antimicrobica lmg 18920t with published 16s rdna sequences from other pseudomonads indicated that pseudomonas antimicrobica belongs to the genus burkholderia, with burkholderia gladioli, burkholderia glumae and burkholderia plantarii as its closest neighbours. dna-dna hybridizations confirmed that pseudomonas antimicrobica and burkholderia gladioli represent the same species. strain lmg 18920t and other burkholderia gladioli strains were also in ...200011155989
burkholderia gladioli: recurrent abscesses in a patient with cystic fibrosis.we describe a case of an adult patient with cystic fibrosis who developed chronic pulmonary infection and multiple episodes of soft tissue abscesses with burkholderia gladioli; this organism should be added to the list of potential pathogens for individuals with cystic fibrosis.200111243758
burkholderia cepacia genomovar vi, a new member of the burkholderia cepacia complex isolated from cystic fibrosis patients.a polyphasic taxonomic study was performed on 23 strains isolated from cystic fibrosis (cf) patients in the usa. these strains were tentatively identified as burkholderia cepacia, burkholderia vietnamiensis and burkholderia or ralstonia sp. using biochemical tests and 16s rdna-based pcr assays. visual comparison of protein profiles indicated that they belonged to a single new group ('group 13'). the polyphasic taxonomic data showed that 18 of these strains represent a new member of the b. cepaci ...200111321071
the potential biocontrol agent pseudomonas antimicrobica inhibits germination of conidia and outgrowth of botrytis cinerea.antifungal metabolites of pseudomonas antimicrobica have previously been shown to inhibit conidial germination of the grey mould pathogen botrytis cinerea. in this study, metabolites of the bacterium have been tested at different stages of botrytis germination to determine their effects on germ tube production and extension.200111328503
[burkholderia cepacia: dangers of a phytopathogen organism for patients with cystic fibrosis].burkholderia cepacia is an environmental bacterium, capable of colonising vegetal and animal tissues, involved in human opportunist nosocomial infections, and above all, in pulmonary colonisations in patients with cystic fibrosis. in these patients, infection may be followed by a severe deterioration with bacteraemia, leading to death. moreover, owing to the epidemic spread of some clones within cystic fibrosis communities, strict preventive guidelines have to be instituted. early detection of b ...200111397673
pcr-based detection and identification of burkholderia cepacia complex pathogens in sputum from cystic fibrosis patients.pcr amplification of the reca gene followed by restriction fragment length polymorphism (rflp) analysis was investigated for the rapid detection and identification of burkholderia cepacia complex genomovars directly from sputum. successful amplification of the b. cepacia complex reca gene from cystic fibrosis (cf) patient sputum samples containing b. cepacia genomovar i, burkholderia multivorans, b. cepacia genomovar iii, burkholderia stabilis, and burkholderia vietnamiensis was demonstrated. in ...200111724828
identification of pandoraea species by 16s ribosomal dna-based pcr assays.the recently described genus pandoraea contains five named species (pandoraea apista, pandoraea pulmonicola, pandoraea pnomenusa, pandoraea sputorum, and pandoraea norimbergensis) and four unnamed genomospecies. pandoraea spp. have mainly been recovered from the respiratory tracts of cystic fibrosis (cf) patients. accurate genus- and species-level identification by routine clinical microbiology methods is difficult, and differentiation from burkholderia cepacia complex organisms may be especiall ...200111724860
burkholderia gladioli keratitis associated with consecutive recurrent report a case of burkholderia gladioli keratitis with consecutive endophthalmitis.200212131039
use of 16s rrna gene sequencing for identification of nonfermenting gram-negative bacilli recovered from patients attending a single cystic fibrosis center.during 1999, we used partial 16s rrna gene sequencing for the prospective identification of atypical nonfermenting gram-negative bacilli isolated from patients attending our cystic fibrosis center. of 1,093 isolates of nonfermenting gram-negative bacilli recovered from 148 patients, 46 (4.2%) gave problematic results with conventional phenotypic tests. these 46 isolates were genotypically identified as pseudomonas aeruginosa (19 isolates, 12 patients), achromobacter xylosoxidans (10 isolates, 8 ...200212354883
[determination of dna-dna homology among pseudomonas cocovenenans subsp. farinofermentans with microdilution plate hybridization method].the dna-dna homologies among pseudomonas cocovenenans subsp. farinofermentans and 11 species in genus burkholderia were determined with microdilution plate hybridization method. the results showed: the homologies among p. cocovenenans subsp. farinofermentans, b. gladioli and b. cocovenenans were all over 75%. it was advised that these three species were synonym, and should be renamed as burkholderia gladioli.200112549192
lysogeny and bacteriophage host range within the burkholderia cepacia complex.the burkholderia cepacia complex comprises a group of nine closely related species that have emerged as life-threatening pulmonary pathogens in immunocompromised patients, particularly individuals with cystic fibrosis or chronic granulomatous disease. attempts to explain the genomic plasticity, adaptability and virulence of the complex have paid little attention to bacteriophages, particularly the potential contribution of lysogenic conversion and transduction. in this study, lysogeny was observ ...200312748267
[phenotypic and genotypic characteristics of non fermenting atypical strains recovered from cystic fibrosis patients].we used partial 16s rrna gene (16s dna) sequencing for the prospective identification of nonfermenting gram-negative bacilli recovered from patients attending our cystic fibrosis center (hôpital necker-enfants malades), which gave problematic results with conventional phenotypic tests. during 1999, we recovered 1093 isolates of nonfermenting gram-negative bacilli from 702 sputum sampled from 148 patients. forty-six of these isolates (27 patients) were not identified satisfactorily in routine lab ...200312948761
burkholderia cepacia complex in cystic fibrosis: frequency of strain replacement during chronic infection.persons with cystic fibrosis (cf) are susceptible to chronic pulmonary infection due to certain burkholderia species, but it is not clear whether this typically involves persistent infection with the same strain or sequential infection with distinct strains. we analyzed 1095 burkholderia isolates recovered from serial sputum cultures from 379 patients with cf receiving care in 112 cf treatment centers in the united states. genotyping was performed by random amplified polymorphic dna typing or pu ...200312955638
disturbance of oxidative phosphorylation by an antibioticum produced by pseudomonas cocovenenans. 196013843874
use of 16s rrna gene sequencing for rapid identification and differentiation of burkholderia pseudomallei and b. mallei.burkholderia pseudomallei and b. mallei, the causative agents of melioidosis and glanders, respectively, are designated category b biothreat agents. current methods for identifying these organisms rely on their phenotypic characteristics and an extensive set of biochemical reactions. we evaluated the use of 16s rrna gene sequencing to rapidly identify these two species and differentiate them from each other as well as from closely related species and genera such as pandoraea spp., ralstonia spp. ...200314532197
need to differentiate lethal toxin-producing strains of burkholderia gladioli, which cause severe food poisoning: description of b. gladioli pathovar cocovenenans and an emended description of b. gladioli.burkholderia cocovenenans produces a lethal toxin (bongkrekic acid) that leads to high fatality in food poisoning cases. however, b. cocovenenans was combined in burkholderia gladioli in 1999. b. gladioli was originally described as a phytopathogenic bacteria that sometimes causes pneumonia in humans and that acts as an opportunistic pathogen. we thought that it was clinically dangerous to describe these two species without considering their pathogenicities. from our data of 16s rrna sequence an ...200314695441
outbreak of otitis media caused by burkholderia gladioli infection in immunocompromised athymic nude mouse with severe head tilt due to otitis media was identified. within weeks of identification of this first case, immune-deficient mice of various genotypes from the same facility were similarly affected, and cases from other facilities were found within two months. culture of ear exudate specimens from affected mice yielded bacteria that were initially identified as burkholderia cepacia, a plant pathogen considered an important opportunistic pathogen in persons with cystic fibr ...200415027624
burkholderia gladioli osteomyelitis in association with chronic granulomatous disease: case report and review.we describe a case of insidious small bone osteomyelitis and soft tissue abscess with burkholderia gladioli in a 6-year-old caucasian boy with chronic granulomatous disease. dna sequencing of the 16s ribosomal rna gene confirmed the bacterial identification. clinical cure was achieved with a combination of antimicrobial therapy and surgical debridement. a review of infections caused by burkholderia spp., other than burkholderia cepacia complex, in pediatric patients with chronic granulomatous di ...200516148855
[determination and analysis of cellular fatty acids composition of burkholderia gladioli].to determine and analyse the composition of cellular fatty acids of burkholderia gladioli.200516329611
burkholderia respiratory tract infections in italian patients with cystic fibrosis: molecular characterize by molecular techniques burkholderia strains responsible for respiratory tract infections in cystic fibrosis (cf) patients (children and adults), to assign the burkholderia cepacia complex (bcc) isolates to a genomovar and to assess the presence of cbla and esmr genes in bacteria. unique or sequential burkholderia isolates (n=48) that had been collected from eight cf children and 17 adults over several (4-6) years were investigated; moreover 11 reference strains were analyzed.200616375971
growth in stewart's medium is a simple, rapid and inexpensive screening tool for the identification of burkholderia cepacia complex.ninety-one percent of burkholderia cepacia complex reference strains (66 out of 72) displayed a yellow slope-green butt colour reaction after growth in stewart's medium indicating the oxidation of glucose and the absence of an arginine dihydrolase system. this same colour reaction was observed for burkholderia gladioli and several ralstonia species, but not for pseudomonas aeruginosa, stenotrophomonas, achromobacter, pandoraea and several other gram-negative non-fermenting bacilli. we therefore ...200616386966
the general secretory pathway of burkholderia gladioli pv. agaricicola bg164r is necessary for cavity disease in white button mushrooms.cavity disease in white button mushrooms is caused by burkholderia gladioli pv. agaricicola. we describe the isolation and characterization of six mutants of the strain bg164r that no longer cause this disease on mushrooms. the mutations were mapped to genes of the general secretory pathway (gsp). this is the first report of the association of the type ii secretion pathway with a disease in mushrooms. phenotypes of the six avirulent mutants were the following: an inability to degrade mushroom ti ...200616672503
[melioidosis-like disease: infection with burkholderia cocovenenans as rare differential diagnosis for lymphadenitis colli].neoplasms, autimmune disorders and infectious diseases as differential diagnoses of cervical lymphadenopathies also require the consideration of rare causes.200716755460
evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ...200716822636
cell surface expression of bacterial esterase a by saccharomyces cerevisiae and its enhancement by constitutive activation of the cellular unfolded protein response.yeast cell surface display is a powerful tool for expression and immobilization of biocatalytically active proteins on a unicellular eukaryote. here bacterial carboxylesterase esta from burkholderia gladioli was covalently anchored into the cell wall of saccharomyces cerevisiae by in-frame fusion to the endogenous yeast proteins kre1p, cwp2p, and flo1p. when p-nitrophenyl acetate was used as a substrate, the esterase specific activities of yeast expressing the protein fusions were 103 mu mg(-1) ...200616980424
burkholderia gladioli: five year experience in a cystic fibrosis and lung transplantation center.the impact of infection with burkholderia gladioli in cystic fibrosis, other chronic airway diseases and immunosuppressed patients is unknown.200717137846
phylogenetic study and multiplex pcr-based detection of burkholderia plantarii, burkholderia glumae and burkholderia gladioli using gyrb and rpod order to develop a detection method for the rice pathogens burkholderia plantarii, burkholderia glumae and burkholderia gladioli, the phylogeny of six plant-pathogenic burkholderia species was analysed using the combined nucleotide sequences of gyrb and rpod. b. plantarii, b. glumae and b. gladioli formed tight monophyletic branches supported by high bootstrap probabilities. the high sequence similarity revealed a close phylogenetic relationship between b. glumae and b. plantarii. b. plantari ...200616627650
comparison of alad activities of citrobacter and pseudomonas strains and their usage as biomarker for pb dehydratase (alad) activity has been used in prokaryotes and eukaryotes as a biomarker for environmental lead (pb) exposure and toxicity. microorganisms are sensitive indicators of toxicity at the fundamental level of ecological organization, but bacterial biomarker studies are focused on the pseudomonas strains in group i and e coli. the objectives of the present work were to determine if burkholderia gladioli belonging to group ii, due to its 16srrna similarity, can be us ...200817516138
isolation and characterization of dibenzofuran-degrading serratia marcescens from alkalophilic bacterial consortium of the chemostat.alkalophilic bacterial consortium developed by continuous enrichment in the chemostat in presence of 4-chlorosalicylic acid as sole source of carbon and energy contained six bacterial strains, micrococcus luteus (csa101), deinococcus radiothilus (csa102), csa103 (burkholderia gladioli), alloiococcus otilis (csa104), micrococcus diversus (csa105), micrococcus luteus (csa106), identified by the biolog test method. the strains were tested for utilization of organic compounds in which one of the str ...200717710482
the structure of the o-specific polysaccharide of the lipopolysaccharide from burkholderia gladioli pv. agaricicola.a neutral o-specific polysaccharide containing d-mannose, d-rhamnose and d-galactose was obtained by mild acid hydrolysis of the lipopolysaccharide of the plant pathogenic bacterium burkholderia gladioli pv. agaricicola. by means of compositional analyses and nmr spectroscopy, the chemical repeating unit of the polymer was identified as a linear trisaccharide of the structure shown below, in which the mannose residue was quantitatively acetylated at c-2. [carbohydrate structure: see text]200616516174
the structure of a putative exopolysaccharide of burkholderia gladioli pv. agaricicola.a putative capsular polysaccharide containing d-rhamnose was isolated from the phytopathogenic bacterium burkholderia gladioli pv. agaricicola by phenol/water extraction followed by ultracentrifugation of the separated water phase and gel-permeation chromatography of the thus obtained supernatant. by means of chemical analyses and nmr spectroscopy, the repeating unit of the polymer was shown to be a linear tetrasaccharide with the structure. [carbohydrates: see text].200616330003
burkholderia gladioli associated abscess in a type 1 diabetic patient.burkholderia gladioli, a bacterial pathogen, causes a variety of infectious ailments in immunocompromised individuals. we present a case report of foreign body associated burkholderia gladioli related abscess in an immunocompetent type 1 diabetic patient. relevant medical literature is reviewed to understand this unusual mode of presentation of this bacterium in our patient.200818626540
random tag insertions by transposon integration mediated mutagenesis (tim).transposon integration mediated mutagenesis (tim) is a broadly applicable tool for protein engineering. this method combines random integration of modified bacteriophage mu transposons with their subsequent defined excision employing type iis restriction endonuclease aari. tim enables deletion or insertion of an arbitrary number of bases at random positions, insertion of functional sequence tags at random positions, replacing randomly selected triplets by a specific codon (e.g. scanning) and sit ...200818627780
matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of nonfermenting gram-negative bacilli isolated from cystic fibrosis patients.the identification of nonfermenting gram-negative bacilli isolated from cystic fibrosis (cf) patients is usually achieved by using phenotype-based techniques and eventually molecular tools. these techniques remain time-consuming, expensive, and technically demanding. we used a method based on matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof-ms) for the identification of these bacteria. a set of reference strains belonging to 58 species of clinically relevan ...200818685005
the bacterium burkholderia gladioli strain chb101 produces two different kinds of chitinases belonging to families 18 and 19 of the glycosyl hydrolases.two genes (chia and chib) coding for chitanases a and b (chia and chib) were isolated from the chitinolytic bacterium, burkholderia gladioli strain chb101. chia contains an open reading frame that encodes a protein of 343 amino acids, whereas chib encodes a protein of 307 amino acids. the deduced amino acid sequence of chia showed a high similarity to those of microbial chitinases belonging to family 18 of the glycosyl hydrolases, while chib showed significant sequence similarity to plant chitin ...200116232958
mixtures of plant growth-promoting rhizobacteria enhance biological control of multiple cucumber pathogens.abstract plant growth-promoting rhizobacteria (pgpr) strains inr7 (bacillus pumilus), gb03 (bacillus subtilis), and me1 (curtobacterium flaccumfaciens) were tested singly and in combinations for biological control against multiple cucumber pathogens. investigations under greenhouse conditions were conducted with three cucumber pathogens-colletotrichum orbiculare (causing anthracnose), pseudomonas syringae pv. lachrymans (causing angular leaf spot), and erwinia tracheiphila(causing cucurbit wilt ...199818944848
microbiological and epidemiological features of clinical respiratory isolates of burkholderia gladioli.burkholderia gladioli, primarily known as a plant pathogen, is involved in human infections, especially in patients with cystic fibrosis (cf). in the present study, the first respiratory isolates recovered from 14 french patients with cf and 4 french patients without cf, identified by 16s rrna gene analysis, were tested for growth on b. cepacia selective media, for identification by commercial systems, and for their antimicrobial susceptibilities, and were compared by pulsed-field gel electropho ...200919297595
dna microarray-based detection and identification of burkholderia mallei, burkholderia pseudomallei and burkholderia spp.we developed a rapid oligonucleotide microarray assay based on genetic markers for the accurate identification and differentiation of burkholderia (b.) mallei and burkholderia pseudomallei, the agents of glanders and melioidosis, respectively. these two agents were clearly identified using at least 4 independent genetic markers including 16s rrna gene, flic, motb and also by novel species-specific target genes, identified by in silico sequence analysis. specific hybridization signal profiles all ...200919366627
naturally occurring anti-ifn-gamma autoantibody and severe infections with mycobacterium cheloneae and burkholderia cocovenenans.recently various genetic defects in immunity mediated by interferon gamma (ifn-gamma) have been described, including mutations in the ifn-gamma receptor 1 (ifn-gammar1) and receptor 2 (ifn-gammar2), signal transducer and activator of transcription 1 (stat 1), and interleukin 12 receptor beta 1 (il-12rbeta1), and il-12 p40 genes. these mutations are associated with the occurrence of severe infections with intracellular pathogens especially nontuberculous mycobacteria and vaccine-associated bacill ...200412947000
rapid identification of burkholderia cepacia complex species including strains of the novel taxon k, recovered from cystic fibrosis patients by intact cell maldi-tof mass spectrometry.two approaches based on intact cell matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (ic-maldi-tof ms) have been evaluated in order to discriminate and identify nine former burkholderia cepacia complex (bcc) species, burkholderia contaminans belonging to the novel taxon k, burkholderia gladioli, and the most relevant non-fermentative (nf) gram-negative rods recovered from cystic fibrosis (cf) sputum cultures. in total, 146 clinical isolates and 26 reference strains we ...200919475140
phenotypic and genetic diversity of rice seed-associated bacteria and their role in pathogenicity and biological study the phenotypic and genetic diversity of culturable bacteria associated with rice seed and to asses the antagonistic and pathogenic potential of the isolated bacteria.200919486410
bioactive and structural metabolites of pseudomonas and burkholderia species causal agents of cultivated mushrooms diseases.pseudomonas tolaasii, p. reactans and burkholderia gladioli pv. agaricicola, are responsible of diseases on some species of cultivated mushrooms. the main bioactive metabolites produced by both pseudomonas strains are the lipodepsipeptides (ldps) tolaasin i and ii and the so called white line inducing principle (wlip), respectively, ldps which have been extensively studied for their role in the disease process and for their biological properties. in particular, their antimicrobial activity and t ...200819787100
microbially induced diseases of agaricus bisporus: biochemical mechanisms and impact on commercial mushroom production.the button mushroom, agaricus bisporus (lange) imbach, the most common cultivated mushroom, is susceptible to a wide range of virus, bacterial, and fungal diseases. however, only some diseases were studied for the mechanisms involved in the host-microorganism interaction. this review deals with biochemical mechanisms related to cavity disease (burkholderia gladioli) and to the interaction between a. bisporus and the causal agents responsible for the most severe diseases, namely the bacteria pseu ...201020127233
biosynthesis of the mitochondrial adenine nucleotide translocase (atpase) inhibitor bongkrekic acid in burkholderia gladioli.biosynthetic studies with (13)c-labelled acetates and methionine revealed that the infamous, food-related toxin bongkrekic acid from burkholderia gladioli is a polyketide with acetate-derived beta-branches and a carboxylate terminus derived from the methyl group of an acetate.201020237660
methods for genetic manipulation of burkholderia gladioli pathovar cocovenenans.abstract:201021080961
complete genome sequence of burkholderia gladioli bsr3.we report the complete genome sequence of burkholderia gladioli bsr3, isolated from a diseased rice sheath in south korea.201121478339
[determination of guanine plus cytosine content of pseudomonas cocovenenans subsp farinofermantans with two methods and the comparative study].the guanine plus cytosine content (g + c) mol% of pseudomonas cocovenenans subsp farinofermantans, burkholderia gladioli and burkholderia cocovenenans, which were similar in biochemical characteristics, were determined with tm values method and hplc method, and the comparative study of these two methods was discussed. a negative conclusion could not be made from the results. the three bacteria were the same species. the hplc method was more accurate than the tm values method, but the repeatabili ...200012520932
bacteria contributing to behaviour of radiocarbon in sodium acetate-utilising bacterium was isolated and identified from deionised water that was used for flooding of paddy soils in this study's batch culture experiments. bacteria in the deionised water samples formed colonies on agar plates containing [1,2-(14)c] sodium acetate, and the autoradiograms showed that all the colonies were positive for (14)c utilisation. then one of the acetate-utilising bacteria was isolated. the isolate was characterised by phylogenetic analysis, cell morphology, gram s ...201121561944
[sequencing and analysis of 16s rdna sequences for p. cocovenenans subsp farinofermentans].in order to fix the phylogenetic taxonomic position of p. cocovenenans subsp. farinofermentans and describe its relationships with other closed species, seven primers were designed to amplify and sequence the 16s rdna of 4 strains of it by using a clonal sequencing or direct sequencing method. the 16s rdna sequence of the 4 strains were aligned with the 16s rdna sequences of other species in genus burkholderia, and the polygenetic tree was produced by using a clustal v and treeview software. the ...199911938985
burkholderia gladioli - a predictor of poor outcome in cystic fibrosis patients who receive lung transplants? a case of locally invasive rhinosinusitis and persistent bacteremia in a 36-year-old lung transplant recipient with cystic fibrosis.there have been very few reports describing postlung transplant outcomes in patients' infected‚ĀĄcolonized with burkholderia gladioli pretransplant. a case involving a lung transplant recipient with cystic fibrosis who ultimately died as a result of severe rhinosinusitis due to b gladioli infection in the context of postlung transplant immunosuppression is reported.201122059186
in vitro antifungal activity of burkholderia gladioli pv. agaricicola against some phytopathogenic fungi.the trend to search novel microbial natural biocides has recently been increasing in order to avoid the environmental pollution from use of synthetic pesticides. among these novel natural biocides are the bioactive secondary metabolites of burkholderia gladioli pv. agaricicola (bga). the aim of this study is to determine antifungal activity of bga strains against some phytopathogenic fungi. the fungicidal tests were carried out using cultures and cell-free culture filtrates against botrytis cine ...201223208371
identification and enzymatic characterization of acid phosphatase from burkholderia gladioli.the genus burkholderia is widespread in diverse ecological niches, the majority of known species are soil bacteria that exhibit different types of non-pathogenic interactions with plants. burkholderia species are versatile organisms that solubilize insoluble minerals through the production of organic acids, which increase the availability of nutrients for the plant. therefore these bacteria are promising candidates for biotechnological applications.201424713147
two case reports of the successful eradication of new isolates of burkholderia cepacia complex in children with cystic fibrosis.chronic infection with burkholderia cepacia complex (bcc) has a detrimental effect on morbidity and mortality for patients with cystic fibrosis (cf). it is therefore logical to attempt to eradicate new isolates however there is a paucity of information to guide treatment. we report the successful eradication of new isolates of bcc in two children with cf.201627018049
estb from burkholderia gladioli: a novel esterase with a beta-lactamase fold reveals steric factors to discriminate between esterolytic and beta-lactam cleaving activity.esterases form a diverse class of enzymes of largely unknown physiological role. because many drugs and pesticides carry ester functions, the hydrolysis of such compounds forms at least one potential biological function. carboxylesterases catalyze the hydrolysis of short chain aliphatic and aromatic carboxylic ester compounds. esterases, d-alanyl-d-alanine-peptidases (dd-peptidases) and beta-lactamases can be grouped into two distinct classes of hydrolases with different folds and topologically ...200211847270
a novel esterase from burkholderia gladioli which shows high deacetylation activity on cephalosporins is related to beta-lactamases and dd-peptidases.the gene (estb) encoding for a novel esterase (estb) from burkholderia gladioli (formerly pseudomonas marginata) ncppb 1891 was cloned in escherichia coli. sequence analysis showed an open reading frame encoding a polypeptide of 392 amino acid residues, with a molecular mass of about 42 kda. comparison of the amino acid sequence with those of other homologous enzymes indicated homologies to beta-lactamases, penicillin binding proteins and dd-peptidases. the serine residue (ser(75)) which is loca ...200111472796
phylogenetic affiliation of the pseudomonads based on 16s rrna sequence.the broad and vague phenotypic definition allowed the genus pseudomonas to become a dumping ground for incompletely characterized polarly flagellated, gram-negative, rod-shaped, aerobic bacteria, and a large number of species have been accommodated in the genus pseudomonas. the 16s rrna sequences of 128 valid and invalid pseudomonas species, which included almost valid species of the genus pseudomonas listed in the approved lists of bacterial names, were obtained: sequences of 59 species were de ...200010939664
heat stable antimicrobial activity of burkholderia gladioli or1 against clinical drug resistant isolates.drug resistant microbes are a serious challenge to human health. during the search for novel antibiotics/inhibitors from the agricultural soil, a bacterial colony was found to inhibit the growth of clinical isolates including staphylococcus (resistant to amikacin, ciprofloxacin, clindamycin, clinafloxacin, erythromycin, gentamicin and methicillin) and candida (resistant to fluconazole and itraconazole). the culture was identified as burkholderia gladioli and produced at least five different anti ...201222771597
[study on the molecular epidemiology characteristics of pseudomonas cocovenenans subsp. farinofermentans isolated from china with rdna fingerprinting].after the chromosomal dna digestion by bgl ii and ecorv, rdna fingerprinting for pseudomonas cocovenenans subsp. farinofermentans was made using biotin-labeled 16s + 23s rdna as a probe. fifty one strains of p. cocovenenans subsp. farinofermentans isolated from some areas of china were divided into 20 ribotyes, 52.9 percent of which were rt6, 9 and 2. in most cases, there were 2 or more ribotypes in each province or city. though same ribotypes were isolated from different provinces, each ribotyp ...199810682641
multiplex pcr assay for simultaneous detection of six major bacterial pathogens of rice.the aim of this study was to develop a multiplex pcr (mpcr) assay for rapid, sensitive and simultaneous detection of six important rice pathogens: xanthomonas oryzae pv. oryzae, x. oryzae pv. oryzicola, pseudomonas fuscovaginae, burkholderia glumae, burkholderia gladioli and acidovorax avenae subsp. avenae.201626864896
burkholderia cocovenenans (van damme et al. 1960) gillis et al. 1995 and burkholderia vandii urakami et al. 1994 are junior synonyms of burkholderia gladioli (severini 1913) yabuuchi et al. 1993 and burkholderia plantarii (azegami et al. 1987) urakami et al. 1994, respectively.reference strains of burkholderia cocovenenans and burkholderia vandii were compared with strains of other burkholderia species using sds-page of whole-cell proteins, dna-dna hybridization and extensive biochemical characterization. burkholderia gladioli and b. cocovenenans were indistinguishable in the chemotaxonomic and biochemical analyses. burkholderia plantarii and b. vandii had indistinguishable whole-cell protein patterns but the b. vandii type strain differed from b. plantarii strains in ...199910028245
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