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[kinetics of setting up of a human fecal flora in germ free mice and trial of decontamination of antibiotherapy (author's transl)].we have tried in this study to reproduce in germfree mice a bacterial equilibrium observed previously in the gastrointestinal tract of child "l.n.", reared in a plastic isolator. eight bacterial strains were studied. seven had been fortuitously introduced: acinetobacter lwoffi ln1, staphylococcus epidermidis ln2, clostridium perfringens ln3, plectridium anatum ln4, streptococcus bovis ln5, enterobacter cloacae ln6, corynebacterium sp ln7. the eighth strain was purposely administered to the child ...1975174472
changes in the bacterial cecal flora of mice infected with trichuris muris (schrank, 1788).cecal microorganisms of mice were categorized and enumerated weekly during the developmental cycle of infection with the whipworm, trichuris muris. the cecal bacterial population consisted of escherichia coli, proteus spp, acinetobacter lwoffi (mima polymorpha), aerobic lactobacilli, staphylococci, enterococci, and anaerobes (bacteroides, streptococci, and lactobacilli) in control and t. muris-infected mice. the aerobic lactobacilli and the anaerobes constituted the greatest number of organisms ...19761019388
[occurrence of species from acinetobacter genus in clinical material and other sources].the analysis regarded 304 strains of acinetobacter genus isolated from various diagnostic materials, objects from hospital environment and from non-hospital sources (soil, water, various animals). applying api zone system, five species were isolated: acinetobacter juni, (18.42%), acinetobacter baumanii (70.39%), acinetobacter haemolyticus (5.59%), acinetobacter lwoffii (4.6%) and acinetobacter johnsonii (0.99%). most frequently isolated species were present in purulent materials and in samples f ...19921297033
cefdinir (fk482), an orally administered cephalosporin in vitro activity comparison against recent clinical isolates from five medical centers and determination of mic quality control guidelines.cefdinir, a new oral cephalosporin, was compared to cefaclor, cefadroxil, cefixime, and cefuroxime against greater than 5000 recent aerobic clinical isolates. this multicenter study revealed broad-spectrum cefdinir activity against all enterobacteriaceae (mic50s, 0.06-2 micrograms/ml) except enterobacter cloacae, morganella morganii, proteus vulgaris, and serratia marcescens (mic50s, greater than or equal to 4 micrograms/ml). oxacillin-susceptible staphylococci (mic90s, 0.5-2 micrograms/ml), bet ...19921424507
intraocular fluid cultures after primary pars plana vitrectomy.to determine what organisms enter the eye and remain in the eye after pars plana vitrectomy, vitreous cavity aspirates were cultured postoperatively. two of 33 (6%) consecutive eyes undergoing primary pars plana vitrectomy had positive cultures. one sample grew a single colony of staphylococcus epidermidis, the second grew two colonies of acinetobacter lwoffi. neither of these eyes developed endophthalmitis. this study demonstrates that bacteria enter the eye at a low rate during pars plana vitr ...19921463038
[sensitivity of acinetobacter calcoaceticus-baumanni complex and acinetobacter lwoffi to common antibiotics]. 19911799617
elimination of mercury, cadmium and antibiotic resistance from acinetobacter lwoffi and micrococcus sp. at high temperature.resistance determinants for hgcl2 and cdcl2 were eliminated along with a number of antibiotic resistance factors from acinetobacter lwoffi and micrococcus sp. at 44 degrees c. these organisms were orginally resistant to hgcl2, merbromin, cdcl2, pb(no3)2, benzylpenicillin, erythromycin, carbenicillin, tetracycline and sulfadiazine. four different types of mutants from a. lwoffi (type i to iv) and one type of mutant from micrococcus sp. (type v) were obtained, depending on the loss of particular r ...19883397010
late intraphagosomal hydrogen ion concentration favors the in vitro antimicrobial capacity of a 37-kilodalton cationic granule protein of human neutrophil granulocytes.we described previously (w.m. shafer, l.e. martin, and j.k. spitznagel, infect. immun. 45:29-35, 1984) the presence of a 37-kilodalton cationic antimicrobial protein (37k cap) in extracts of granules prepared from human polymorphonuclear granulocytes (pmn). in this investigation, we prepared 37k cap from pmn granule extracts by sequential ion-exchange and molecular-sieve chromatography and examined its antimicrobial activity against a number of gram-negative and gram-positive bacteria. at concen ...19863527987
in vitro activity of imipenem towards gram-negative bacilli.the authors studied the in vitro antimicrobial activity of imipenem towards 355 gram-negative bacterial strains, taking into particular consideration unusual or dangerous species. the study was carried out on a comparative basis with piperacillin, cefotaxime, ceftazidime and gentamicin. ninety percent of the fermentative gram-negative strains were inhibited at concentrations less than or equal to 2 mg/l. imipenem inhibited 100% of strains of alcaligenes faecalis, alcaligenes denitrificans, flavo ...19863466723
the isocitrate dehydrogenases of acinetobacter lwoffi. separation and properties of two nicotinamide-adenine dinucleotide phosphate-linked isoenzymes.two isoenzymes of nadp-linked isocitrate dehydrogenase have been identified in acinetobacter lwoffi and have been termed isoenzyme-i and isoenzyme-ii. the isoenzymes may be separated by ion-exchange chromatography on deae-cellulose, by gel filtration on sephadex g-200, or by zonal ultracentrifugation in a sucrose gradient. low concentrations of glyoxylate or pyruvate effect considerable stimulation of the activity of isoenzyme-ii. the isoenzymes also differ in ph-dependence of activity, kinetic ...19724144072
[a clinical evaluation of sultamicillin fine granules in the treatment of meibomianitis].this study was performed to evaluate the clinical efficacy and the safety of sultamicillin (sbtpc) fine granules in the treatment of patients with meibomianitis. a dose of 375 mg sbtpc granules was orally given to 10 patients with meibomianitis after each meal, three times a day. the results obtained are summarized as follows. efficacies were rated as good in 7 cases, fair in 2 and poor in 1 with an efficacy ratio of 70.0%. no side effects were observed throughout the study. the organisms isolat ...19883249376
taxonomic studies of acinetobacter species based on the electrophoretic analysis of enzymes.thirty six strains of acinetobacter, 22 strains from the culture collections and 14 isolates from soil and cotton samples, were examined for the presence of twelve enzymes using polyacrylamide gel electrophoresis. a numerical analysis was carried out on the basis of similarity values obtained from the electrophoretic mobilities of the enzymes. the strains used were divided into four clusters (z-1, z-2, z-3 and z-4), and cluster z-1 was further divided into three subclusters (z-1a, z-1b and z-1c) ...19883210151
[in vitro activity of seven gyrase inhibitors of a group of heterocyclic carbonic acids against nonfermenting gram negative rods (nonfermenters)].mic (minimal inhibitory concentration) determinations for nalidixic acid, cinoxacin, pipemidic acid, norfloxacin, enoxacin, and ciprofloxacin were done by agar dilution on isosensitest agar (oxoid). bacterial strains investigated were 189 pseudomonas aeruginosa, 164 acinetobacter lwoffii, 4 ps. maltophilia, 3 ps. putrefaciens and 3 ps. odorans. the results in summary are: ciprofloxacin is the most active gyrase inhibitor against ps. aeruginosa as well as against other nonfermentative gram-negati ...19852986655
partial purification and properties of the fructose-1,6-diphosphatase from acinetobacter lwoffi. 19714322806
a unique type ii restriction endonuclease from acinetobacter lwoffi n. 19872821501
isolation of acinetobacter lwoffi from hens with septicemia.acinetobacter lwoffi was isolated from the lungs of two hens. the isolate was sensitive to chloramphenicol, ampicillin, erythromycin, oxytetracycline but resistant to penicillin and nitrofurantoin.19892750363
acinetobacter calcoaceticus genes involved in biosynthesis of the coenzyme pyrrolo-quinoline-quinone: nucleotide sequence and expression in escherichia coli k-12.synthesis of the coenzyme pyrrolo-quinoline-quinone (pqq) from acinetobacter calcoaceticus requires the products of at least four different genes. in this paper we present the nucleotide sequence of a 5,085-base-pair dna fragment containing these four genes. within the dna fragment three reading frames are present, coding for proteins of mr 10,800, 29,700, and 43,600 and corresponding to three of the pqq genes. in the dna region where the fourth pqq gene was mapped the largest possible reading f ...19892536663
effects of photo-oxidation on the catalytic and regulatory properties of citrate synthase from acinetobacter lwoffi. 19744368651
[antibiotic resistance of gram-negative bacteria isolated at a hospital intensive care unit].the antibiotic sensitivity of gram-negative facultatively pathogenic bacteria isolated between 1982-1986 in the respiratory intensive care unit of the 3rd department of pediatry of the "lászló" hospital (budapest) has been studied. pseudomonas aeruginosa, klebsiella, acinetobacter calcoaceticus and acinetobacter lwoffii were most frequently isolated both from the clinical samples (total 56.9%) and in samples obtained from hospital-hygienic check-examinations. 70-100% of the individual species wa ...19892498804
[clinical examination of ceftibuten in acute bronchitis].clinical evaluation of ceftibuten (cetb, 7432-s) was performed in 20 patients with acute bronchitis. they were consisted of 10 males and 10 females aged from 20 to 80 years old. cetb was given orally in daily dose of 300 mg (18 cases) or 600 mg (2 cases) in three divided portions. the duration of administration was 3 to 14 days. especially they were given for 7 days in 16 cases. a total of 11 strains comprising 4 strains of staphylococcus aureus, 2 strains of beta-streptococcus and 1 strain each ...19902391747
[clinical evaluation of cefpirome in children].cefpirome (cpr, hr 810) was clinically evaluated for its efficacy and safety in 11 patients with ages from 4 months to 11 years with bacterial infection. the results obtained are summarized as follows. 1. cpr was administered to 6 patients with bronchopneumonia, a patient with pneumonia, a patient with tonsillitis, 2 patients with acute pharyngitis and a patient with suppurative parotitis at daily dosage levels ranging 55.5-91.7 mg/kg, divided into 3 using intravenous bolus injection or 30 minut ...19911880923
trauma-induced acinetobacter lwoffi endophthalmitis. 19921575244
[cerebrospinal meningitis in fez in 1966-1967. ii. the fez epidemic. bacteriological considerations. c. study of 53 strains of moraxella lwoffi and moraxella glucidolytica isolated during the epidemic]. 19695403105
[antibiotic therapy of infections in patients on dialysis].the results obtained in treating infections in 53 patients involved in a chronic dialysis-transplantation program are reported. higher susceptibility to infections and reduced immune functions were observed in dialyzed patients. the problem of the occurrence rate of individual infections was analyzed and infections of the respiratory system, particularly bronchopneumonia, were found to be predominant. no patient died of infection; in two patients the prescribed antibiotic of first choice had to ...19921525680
[meningitis caused by mima polymorpha (acinetobacter lwoffii)]. 19675618957
susceptibility of isolates of acinetobacter anitratus and acinetobacter lwoffii to the bactericidal activity of normal human serum.the bactericidal activity of normal human serum against the gram-negative coccobacilli acinetobacter anitratus and acinetobacter lwoffii was studied; 12% and 84%, respectively, of the tested strains appeared to be sensitive. thus, serum resistance may be an important factor contributing to the pathogenic potential of a. anitratus strains. three types of bactericidal action were shown. in the first, the strains were killed when the alternative complement pathway was activated. in the second, some ...19921515154
biochemical similarity of acinetobacter lwoffii and acinetobacter anitratus. 19685645620
[the moraxella lwoffi group of bacteria (author's transl)]. 19761021821
structure of the peptidoglycans of moraxella glucidolytica and moraxella lwoffi grown on hydrocarbons.the peptidoglycans of moraxella glucidolytica and moraxella lwoffi grown on aliphatic hydrocarbons were isolated. they contained muramic acid, glucosamine, alanine, d-glutamic acid and mesodiaminoimelic acid in a molar ratio of about 0.5:0.5:1.6:1.0:1.0 (m. glucidolytica) and 0.8:0.7:1.3:1.0:1.0 (m. lwoffi). the peptidoglycans were lysozyme-resistant. however, when treated with formanide, they could be partially degraded by lysozyme. the fragments were purified and their structure determined. in ...1977871229
[bacterial flora of the conjunctival sac of the horse].the aa. report the results of taxonomic research conducted on the conjunctival sac of 59 horses for identification of the present bacterial flora. in the controlled animals, it was observed, at the level of the considered niche, a community constituted of normal bacterial populations, but not autochtonous in the significance they attributed from dubos et al., relative to the characterization of the indigenous microbiota of the intestine. the isolated normal bacterial flora seems to be constitute ...1976788654
metabolism of bile acids by strains of acinetobacter anitratum and acinetobacter lwoffii.the activity of two species of bacteria: acinetobacter anitratum and acinetobacter lwoffii, isolated from jejunum of patients with achlorhydria and reichel-polya resection towards bile salts was investigated. these species were not previously tested from this view point. all strains tested hydrolyzed bile salts; conjugates of deoxycholic acid were hydrolyzed more readily than those of cholic acid. this feature may be used as a bacteriological taxonomic criterion.1978685951
[bronchial brushing in severe lung diseases in children].a distal bronchial brushing was performed in 25 children having severe lung diseases. the age of the patients ranged from 7 days to 14 years. seventeen children were immuno-depressed. bacteria were isolated five times (2 staphylococcus aureus, 1 streptococcus pneumoniae, 1 serratia marcenscens, 1 acinetobacter lwoffi), viruses twice (cytomegalovirus, syncytial respiratory virus), pneumocystis carinii, four times. cytologic study revealed malignant cells, and cells with intranuclear inclusions, e ...1978637669
stimulation of lipase production during bacterial growth on alkanes.acinetobacter lwoffi strain o(16), a facultative psychrophile, can grow on crude oil, hexadecane, octadecane, and most alkanes when tested at 20 but not at 30 degrees c. growth occurred on a few alkanes at 30 degrees c but after a longer lag than at 20 degrees c. cells grown on alkanes as sole carbon sources had high levels of cell-bound lipase. in contrast, previous work has shown that those grown on complex medium produced cell-free lipase and those grown on defined medium without alkanes prod ...1978627533
semiquantitative catalase test as an aid in identification of oxidative and nonsaccharolytic gram-negative bacteria.a simple and rapid semiquantitative slide catalase test useful for the identification of oxidative and nonsaccharolytic gram-negative bacteria, i.e., "nonfermenters," is described. using the interpretative criterion of time of appearance of oxygen bubbles in 3% hydrogen peroxide, three categories of nonfermenters were established. the rapid catalase producers included achromobacter xylosoxidans and achromobacter species; acinetobacter anitratus and acinetobacter lwoffii; bordetella bronchiseptic ...1979393719
nonfermentative bacilli: evaluation of three systems for identification.three systems for the identification of nonfermentative bacilli were evaluated for their rapidity and accuracy of identification of 217 strains. two of the systems, api 20e (api) and oxi/ferm tube (oxif), are available as kits; the oxidative attack (oa) system is not commerically available. the overall accuracies of the oa, api, and oxif systems were 91, 69, and 50%, respectively. identification within 48 h was achieved for 98% of the strains by oa, for 50% by api, and for 18% by oxif. most of t ...1979389945
purification and properties of malate-nad+ dehydrogenase of moraxella lwoffi (n.c.i.b. 8250). 1976191315
the degradation of 1-phenylalkanes by an oil-degrading strain of acinetobacter lwoffi.an oil-degrading bacterium identified as acinetobacter lwoffi was isolated by elective culture on north sea forties crude oil from an activated sludge sample. it grew on a wide range of n-alkanes (c12-c28) and 1-phenylalkanes, including 1-phenyldodecane, 1-phenyltridecane and 1-phenyltetradecane. the organism degraded 1-phenyldodecane to phenylacetic acid which was further metabolized via homogentisic acid, whilst 1-phenyltridecane was transformed to trans-cinamic and 3-phenylpropionic acid whic ...19854004202
in vitro susceptibility of acinetobacter species to various antimicrobial agents.the in vitro activity of 18 antimicrobial agents against 40 clinical isolates each of acinetobacter calcoaceticus subsp. anitratum and acinetobacter lwoffi was studied. most of the newer 4-quinolone derivatives were extremely active against these organisms. newer beta-lactam agents, such as cefpirome, bmy 28142, and brl 36650, were also extremely active, inhibiting all strains at clinically achievable levels. most agents were two- to fourfold more active against a. lwoffi.19863800353
degradation of substituted mandelic acids by meta fission reactions.a strain of acinetobacter lwoffii degraded 4-hydroxymandelic and 4-hydroxy-3-methoxymandelic acids to their corresponding benzoates, which were then hydroxylated by specific monooxygenases to yield, respectively, protocatechuic and 3-o-methylgallic acids; these were substrates for meta fission dioxygenases. the product formed from 3-o-methylgallate underwent slow spontaneous cyclization at ph 7 to release methanol.19873611031
the isocitrate dehydrogenases of acinetobacter lwoffi. studies on the regulation of a nicotinamide-adenine dinucleotide phosphate-linked isoenzyme.of the two nadp-linked isocitrate dehydrogenases in acinetobacter lwoffi the higher-molecular-weight form, isoenzyme-ii, is reversibly stimulated sixfold by low concentrations of glyoxylate or pyruvate. kinetic results indicate that this stimulation of activity involves both an increase in v(max.) and a decrease in the apparent k(m) values for substrates, most markedly that for nadp(+). other changes brought about by glyoxylate or pyruvate include a shift in the ph optimum for activity and an in ...19734146835
terminal respiration in moraxella lwoffi (ncib 8250). 19714147905
a direct pathway for the conversion of propionate into pyruvate in moraxella lwoffi.1. the identity of the organism previously known as vibrio o1 (n.c.i.b. 8250) with a species of moraxella is established. 2. the ability of cells to oxidize propionate is present only in cells with an endogenous respiration and this ability is increased 80-fold when the organism is grown with propionate. 3. isocitrate lyase activity in extracts from propionate-grown cells is the same as that in extracts from lactate-grown cells, about tenfold greater than that in extracts from succinate-grown ce ...19684296055
[fetal death in utero due to moraxella lwoffi placental infection]. 19724338409
particulate malate oxidation in strictly aerobic bacteria: the respiratory system of moraxella lwoffi. 19724342500
antibacterial activity of bms-180680, a new catechol-containing monobactam.the in vitro activities of a new catechol-containing monobactam, bms-180680 (sq 84,100), were compared to those of aztreonam, ceftazidime, imipenem, piperacillin-tazobactam, ciprofloxacin, amikacin, and trimethoprim-sulfamethoxazole. bms-180680 was often the most active compound against many species of the family enterobacteriaceae, with mics at which 90% of the isolates were inhibited (mic90s) of < or = 0.5 microg/ml for escherichia coli, klebsiella spp., citrobacter diversus, enterobacter aero ...19979145861
infectious crystalline keratopathy caused by gram-negative bacteria.to identify the characteristics and outcomes of infectious crystalline keratopathy caused by gram-negative bacteria.19979222227
the purification and regulatory properties of alpha-oxoglutarate dehydrogenase from acineobacter iwoffi.the alpha-oxoglutarate dehydrogenase multienzyme complex was purified from acinetobacter lwoffi to a high degree of homogeneity as shown by gel electrophoresis and analytical ultracentrifugation. sedimentation-velocity analyses gave s(20,w) values which increased with increasing protein concentration, suggesting dissociation of the complex in dilute solution. the maximum s(20,w) value thereby obtained and the value determined by active enzyme centrifugation were both in the range 28-29s. electro ...19734359921
utilization of acetylsalicylic acid as sole carbon source and the induction of its enzymatic hydrolysis by an isolated strain of acinetobacter lwoffii. 19704396112
distribution of acinetobacter species on human skin: comparison of phenotypic and genotypic identification methods.at least 19 genomic species are recognized as constituting the genus acinetobacter. however, little is known about the natural reservoirs of the various members of the genus. an epidemiological study was therefore performed to investigate the colonization with acinetobacter spp. of the skin and mucous membranes of 40 patients hospitalized in a cardiology ward and 40 healthy controls. single samples were obtained once from each of nine different body sites, i.e., forehead, ear, nose, throat, axil ...19979350741
the degradative versatility, arylesterase activity and hydroxylation reactions of acinetobacter lwoffi ncib 10553. 19734717956
[post-traumatic arthritis caused by pseudomonas aeruginosa and moraxella lwoffi: identification and pathogenic role of moraxella]. 19715000089
degradation of acetylsalicylic acid by a strain of acinetobacter lwoffii. 19715004245
nucleic acid homologies among oxidase-negative moraxella species.the deoxyribonucleic acid (dna) base composition and dna homologies of more than 40 strains of oxidase-negative moraxella species were determined. these bacteria have also been identified as belonging to the mima-herellea-acinetobacter group and the bacterium anitratum group, as well as to several other genera including achromobacter and alcaligenes. the dna base content of these strains ranged from 40 to 46% guanine plus cytosine. dna-dna competition experiments distinguished five groups whose ...19705413826
a direct pathway for the metabolism of propionate in cell extracts from moraxella lwoffi.1. extracts from moraxella lwoffi oxidize propionate, but at a low rate when compared with whole cells. 2. this oxidative activity requires the formation of propionyl-coa. 3. enzymes catalysing the formation of propionyl phosphate and propionyl-coa are present. the presence of a propionyl-coa hydrolase is considered to be an artifact, but partly responsible for the low rates of oxidation. 4. enzymes catalysing the reduction of nad(+) and the formation of pyruvate with propionyl-coa as substrate ...19685642619
exceptional desiccation tolerance of acinetobacter radioresistens.the taxonomy of the genus acinetobacter, which includes several important nosocomial pathogens, has been confused due to a lack of discriminatory phenotypic characteristics for identification. molecular methods such as amplified ribosomal dna restriction analysis (ardra) now enable the accurate identification of species. ten clinical isolates of acinetobacter radioresistens had genospecies confirmed by ardra but the apj 20ne system, commonly used in clinical microbiology laboratories, mis-identi ...19989699144
development and evaluation of a coculture technique for identification of anaerobic organisms.we investigated a method to reduce oxygen tension and produce anaerobic conditions conveniently by coculture with a nonfermentative organism. the biochemical reactions of 94 strains representing 41 different species were determined using prereduced media and gas chromatography according to standard methods. the standard reactions were compared with those obtained by preparing similar media, modified by containing no cysteine or resazurin, under aerobic conditions. preincubation of the modified b ...19846365960
acinetobacter bacteremia in hong kong: prospective study and review.the epidemiological characteristics of 18 patients with acinetobacter bacteremia were analyzed. patients (mean age, 55.5 years) developed bacteremia after an average of 14.1 days of hospitalization. fifteen of 16 patients survived bacteremia caused by acinetobacter baumannii. cultures of blood from the remaining two patients yielded acinetobacter lwoffii. most patients (78%) resided in the general ward, while four patients (22%) were under intensive care. genotyping by arbitrarily primed polymer ...199910028065
nonfermenting gram-negative bacteria and their differentiation from pseudomonas aeruginosa.pseudomonas aeruginosa strains and other nonfermenting gram-negative bacteria isolated from surgical patients and environmental washings at a surgical in-patient department were characterized bacteriologically. the biochemical and cultivation features of 156 p. aeruginosa strains, 35 strains of oxidase-negative and 31 strains of oxidase-positive gram-negative bacteria other than p. aeruginosa were studied. nonpigmented p. aeruginosa strains and strains with delayed pigmentation accounted for, re ...19827161487
[the identification of nonfermentative gram-negative bacteria. experiences with 676 apyocyaninogenic strains (author's transl)].during a period of 16 months 1757 strains of nonfermentative gram-negative rods have been isolated from clinical material. of the, 1205 (69%) were p. aeruginosa, 124 (10%) of which failed to produce pyocyanin. the apyocyaninogenic strains as well as the remaining 552 isolates were differentiated by steps according to a diagnostic scheme developed by us. for identification of species two or three steps were needed. by this procedure, 530 of the 552 strains could be assigned to nineteen species wi ...19817223132
phylogenetic relationships between some members of the genera neisseria, acinetobacter, moraxella, and kingella based on partial 16s ribosomal dna sequence analysis.we obtained 16s ribosomal dna (rdna) sequence data for strains belonging to 11 species of proteobacteria, including the type strains of kingella kingae, neisseria lactamica, neisseria meningitidis, moraxella lacunata subsp. lacunata, [neisseria] ovis, moraxella catarrhalis, moraxella osloensis, [moraxella] phenylpyruvica, and acinetobacter lwoffii, as well as strains of neisseria subflava and acinetobacter calcoaceticus. the data in a distance matrix constructed by comparing the sequences suppor ...19947520730
detection of alpha/beta-hydrolase fold in the cell surface esterases of acinetobacter species using an analysis of 3d profiles.the primary sequence of esterases from acinetobacter lwoffii rag-1 and a. calcoaceticus bd413 were compared with linearized structural sequences of two hundred proteins selected from brookhaven protein databank using a modified version of the bowie et al. algorithm [3]. significant structural homology was found to alpha/beta proteins and specifically to those with the alpha/beta-hydrolase fold for which the crystal structure was reported. no such homology was detected using common primary sequen ...19957556598
treatment of sanitary-important bacteria by bacteriocin substance v24 in cattle dung water.quantification of sanitary-important bacteria (e.g. enterobacteriaceae), as well as indicators of environmental contamination, was assessed in samples of cattle dung from 25 cattle farms in 15 north-eastern slovakia districts. the inhibitory effect of crude bacteriocin extract cbe v24 from enterococcus faecalis v24 against listeria monocytogenes ohio and yersinia enterocolitica ye85 was examined in cattle dung water with the aim of finding a new way of eliminating the health risk of the animal s ...200010792671
bacterial pneumonia in recipients of bone marrow transplantation. a five-year prospective study.bacterial pneumonia as an important complication of bone marrow transplantation (bmt) has not been subjected to comprehensive analysis. two hundred fifty-five consecutive allogeneic and autologous bmt recipients, ranging in age from 1 month to 53 years, were prospectively followed for 3 days to 3 years (median, 108 days) for development of bacterial pneumonia. etiology, place acquired, chest radiography, and outcome were recorded and the association between bacterial pneumonia and demographic an ...19957570975
gut bacteria provide precursors of benzodiazepine receptor ligands in a rat model of hepatic encephalopathy.benzodiazepine receptor (bzr) ligands are elevated in animals and humans with fulminant hepatic failure (fhf) and contribute to the pathogenesis of the associated hepatic encephalopathy (he). as gut factors are proposed to play a role in the pathogenesis of he, we investigated gut flora as a source of bzr ligands. rats received daily oral neomycin, vancomycin and metronidazole (ab +) or saline (ab -) before and concurrent with the induction of fhf with thioacetamide. bzr ligands were extracted f ...19957648264
isolation, characterization, and sequence analysis of cryptic plasmids from acinetobacter calcoaceticus and their use in the construction of escherichia coli shuttle plasmids.three cryptic plasmids have been discovered in acinetobacter calcoaceticus bd413. these three plasmids, designated pwm10 (7.4 kb), pwm11 (2.4 kb), and pwm12 (2.2 kb), exhibited extensive homology to one another, as shown by southern blot hybridization and restriction site analysis data, and also hybridized with three plasmids having slightly different sizes detected in a second strain, a. calcoaceticus bd4. plasmid pwm11 and a fragment of pwm10 were each subcloned into puc19, yielding plasmids p ...19938215356
esterase from the oil-degrading acinetobacter lwoffii rag-1: sequence analysis and over-expression in escherichia coli.the est gene encoding an esterase from acinetobacter lwoffii rag-1 was cloned into e. coli under the control of the pl promoter of the phage lambda. the n-terminal sequence of the first 20 amino acids of the heterologous expressed esterase corresponded to that obtained from the nucleotide sequence. antibodies prepared against the over-expressed recombinant esterase in e. coli were used to locate the enzyme primarily in the membrane fractions of a. lwoffii rag-1. comparison with homologous protei ...19938224790
infective endocarditis of an interventricular patch caused by acinetobacter haemolyticus.a case of infective endocarditis caused by acinetobacter haemolyticus affecting and interventricular patch is reported. the patient, a 21-year-old man with a fallot's tetralogy who had undergone cardiovascular surgery several years before, received imipenem and gentamicin for 6 and 4 weeks respectively and showed a good response without needing surgical treatment. endocarditis by acinetobacter species is very unusual and, to our knowledge, this is the first reported case of infective endocarditi ...19958522385
evaluation of the ability of a commercial system to identify acinetobacter genomic species.a collection of 130 acinetobacter strains identified by dna hybridization to 18 different genomic species was used to assess the ability of the api 20ne system (biomérieux, france) to identify acinetobacter genomic species and to determine its accuracy. fifty-eight (87%) of the 67 strains of genomic species defined in the database (version 5.1) were identified to the appropriate genomic species. the acinetobacter baumannii strains and the acinetobacter haemolyticus strains were all identified co ...19968781881
characterization of the chromosomal cephalosporinases produced by acinetobacter lwoffii and acinetobacter baumannii clinical isolates.the beta-lactamases produced by acinetobacter lwoffii ula-501, acinetobacter baumannii ula-187, and a. baumannii ac-14 strains were purified and characterized, and their kinetic interactions with several beta-lactam molecules, including substrates and inhibitors, were studied in detail. the three enzymes appeared to be cephalosporinases with different acylation efficiencies (kcat/km ratio values), and their hydrolytic activities were inhibited by benzylpenicillin, piperacillin, and cefotaxime, w ...19968851599
resistance to antibiotics and biocides among non-fermenting gram-negative bacteria.to investigate the antibiotic and biocide susceptibilities of clinical isolates of rarely encountered gram-negative, non-fermenting bacteria.200111442564
comparative in vitro activity of gemifloxacin, ciprofloxacin, levofloxacin and ofloxacin in a north american surveillance study.the in vitro activity of gemifloxacin, a new fluoroquinolone, was compared to three marketed fluoroquinolones; ciprofloxacin, levofloxacin and ofloxacin against over 4,000 recent clinical isolates covering 29 species isolated in the united states and canada between 1997-1999. based on mic(90)s, gemifloxacin was the most potent fluoroquinolone tested against a majority of gram-positive isolates: streptococcus pneumoniae, penicillin resistant s. pneumoniae, macrolide resistant s. pneumoniae, cipro ...200111448564
utilization and degradation of an ester-based synthetic lubricant by acinetobacter lwoffi.an oil-degrading bacterium, acinetobacter lwoffi, isolated by elective culture from the medway estuary, utilized an ester-based synthetic lubricating oil emkarate de 155 as sole carbon and energy source. analysis of culture supernatants by gas chromatography showed the accumulation of a nondegradable metabolite 1,1,1 tris (hydroxymethyl)propane in addition to two metabolizable fatty acids, octanoic and decanoic acids as products of the synthetic oil degradation. esterase activities were subseque ...19968882802
phosphate release and uptake by pure cultures of acinetobacter sp.: effect of the volatile fatty acids concentrationphosphorus release and uptake by pure cultures of acinetobacter strains were investigated under anaerobic and aerobic conditions respectively. tests were performed to study the relationship between phosphorus release-storage reaction and behavior of extracellular organic substrates: acetic, propionic, and butyric acids have been used at four concentrations (50, 100, 500, and 1000 mg &middot; l-1) in the anaerobic step of biological phosphorus removal. the results obtained depend on the strain an ...19978939801
cloning and characterization of two cata genes in acinetobacter lwoffii k24.two novel type i catechol 1,2-dioxygenases inducible on aniline media were isolated from acinetobacter lwoffii k24. although the two purified enzymes, cd i1 and cd i2, had similar intradiol cleavage activities, they showed different substrate specificities for catechol analogs, physicochemical properties, and amino acid sequences. two cata genes, cata1 and cata2, encoding by cd i1 and cd i2, respectively, were isolated from the a. lwoffii k24 genomic library by using colony hybridization and pcr ...19979260969
discrimination of acinetobacter genomic species by aflp fingerprinting.aflp is a novel genomic fingerprinting method based on the selective pcr amplification of restriction fragments. the usability of this method for the differentiation of genomic species in the genus acinetobacter was investigated. a total of 151 classified strains (representing 18 genomic species, including type, reference, and field strains) and 8 unclassified strains were analyzed. by using a single set of restriction enzymes (hindiii and taqi) and one particular set of selective pcr primers, a ...19979336926
peptide mapping and amino acid sequencing of two catechol 1,2-dioxygenases (cd i1 and cd i2) from acinetobacter lwoffii k24.the partial amino acid sequences of two catechol 1,2-dioxygenases (cd i1 and cd i2) from acinetobacter lwoffii k24 have been determined by analysis of peptides after cleavages with endopeptidase lys-c, endopeptidase glu-c, trypsin, and chemicals (cyanogen bromide and bnps-skatole). they include 248 amino acid sequences (4 fragments) of cd i1 and 211 amino acid sequences (5 fragments) of cd i2. two enzymes have more than 50% sequence homology with type i catechol 1,2-dioxygenases and less than 30 ...19979387151
bacterial flora of hirudo medicinalis and their antibiotic sensitivities in the middle black sea region, turkey.the rate of infectious complications of leech therapy is almost 20% because hirudo medicinalis has endosymbiotic bacteria. the aim of this study was to determine the bacterial flora of h. medicinalis and their antibiotic sensitivities in a region in turkey. sixteen adult leeches were collected in middle black sea region, turkey. they were rubbed onto blood agar plates directly under ether anesthesia to obtain surface cultures. they were then killed to obtain mouth and gut cultures. culture swabs ...200111756807
evaluation of vitek gni+ and becton dickinson microbiology systems crystal e/nf identification systems for identification of members of the family enterobacteriaceae and other gram-negative, glucose-fermenting and non-glucose-fermenting bacilli.we evaluated the vitek gni+ and becton dickinson crystal e/nf identification systems for their ability to accurately identify 619 and 626 strains, respectively, of members of the family enterobacteriaceae and other glucose-fermenting and non-glucose-fermenting gram-negative rods. all strains tested were taken from a stock collection and passed three times on 5% sheep blood agar prior to testing. these strains represented a more rigorous challenge to both systems than one resulting from the testi ...19979399532
organization and transcriptional characterization of the cat1 gene cluster in acinetobacter lwoffi k24.previously, we have reported that two clustered cat genes from acenitobacter lwoffi k24 had different arrangements, catb1c1a1 and catb2a2c2 (kim, s.i., s.-h. leem, j.-s. choi, y.h. chung, s. kim, y.-m. park, y.k. park, y.n. lee, and k.-s. ha. 1997, j. bacteriol. 179, 5226-5231). by further analysis of the organization of the cat1 gene cluster, we obtained a complete sequence of the catb1 gene, which encoded 40.8-kda polypeptide containing 379 amino acids, and found a open reading frame (orf) cod ...19989473520
[post-marketing surveillance of antibacterial activities of cefozopran against various clinical isolates--ii. gram-negative bacteria].as a post-marketing surveillance, the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates were yearly evaluated and compared with those of other cephems, oxacephems, penicillins, monobactams, and carbapenems. changes in czop susceptibility for the bacteria were also evaluated with the bacterial resistance ratio calculated with the breakpoint mic. twenty-five species (3,362 strains) of gram-negative bacteria were isolated from the clinica ...200211977920
trauma-induced acinetobacter lwoffi endophthalmitis with multi-organism recurrence: strategies with intravitreal treatment.endophthalmitis and its treatment are challenging, controversial subjects. we report here a patient whose recurrent endophthalmitis required several modes of treatment.19979537147
antimicrobial multiresistance in bacteria isolated from freshwater chilean salmon farms.the intensive use of antimicrobial agents, mainly oxytetracycline, to prevent and control bacterial pathologies in chilean salmon culture is a frequent practice. a total of 103 gram-negative oxytetracycline-resistant bacteria recovered from various sources of 4 chilean freshwater salmon farms were identified and investigated for their susceptibility patterns to various antibacterial agents, by using an agar disk diffusion method. antibacterial resistance patterns of isolates were not correlated ...200212109474
identification of acinetobacters on blood agar in presence of d-glucose by unique browning effect.a positive phenotypic characteristic of glucose-oxidizing acinetobacters was demonstrated with blood agar containing d-glucose. glucose-oxidizing acinetobacter baumannii, acinetobacter genospecies 3, acinetobacter lwoffii, and acinetobacter genospecies 13 sensu tjernberg and ursing caused a unique brown discoloration of media supplemented with 5% blood (of horse, sheep, or human origin) and an aldose sugar (0.22 m d-glucose, d-galactose, d-mannose, d-xylose, or lactose). the browning effect was ...19989574714
effect of desiccation on the ultrastructural appearances of acinetobacter baumannii and acinetobacter lwoffii.an acinetobacter baumannii isolate survived desiccation beyond 30 days and an acinetobacter lwoffii isolate up to 21 days. for both species, desiccation resulted in a significant increase in the proportion of round cells (a baumannii, 40% to 80%; a lwoffii, 51% to 63%) and a significant decrease in rod shaped cells (a baumannii, 58% to 13%; a lwoffii, 46% to 34%). electronmicroscopic examination showed that there was also a corresponding significant increase in the cell wall thickness (a baumann ...199810023344
distribution of acinetobacter species on skin of healthy humans.the distribution of the 19 currently known genospecies of acinetobacter on human skin, i.e. forehead, forearm and toe webs, was determined. three selective media were compared for their specificity for all genospecies of acinetobacter. a minimal-salts agar supplemented with 1% acetate proved to be more efficient than the leeds medium for the isolation of most genospecies in mixed culture with other bacterial species. acinetobacter isolates were provisionally identified using biochemical tests an ...199910357050
involvement of a protein tyrosine kinase in production of the polymeric bioemulsifier emulsan from the oil-degrading strain acinetobacter lwoffii rag-1.the genes associated with the biosynthesis of the polymeric bioemulsifier emulsan, produced by the oil-degrading acinetobacter lwoffii rag-1 are clustered within a 27-kbp region termed the wee cluster. this report demonstrates the involvement of two genes of the wee cluster of rag-1, wzb and wzc, in emulsan biosynthesis. the two gene products, wzc and wzb were overexpressed and purified. wzc exhibited atp-dependent autophosphorylating protein tyrosine kinase activity. wzb was found to be a prote ...200312533476
microbial contamination of non-disposable instruments in otolaryngology out-patients.nosocomial infections are an important cause of morbidity and contaminated equipment may contribute to this. there has been little work concerning contamination of non-disposable equipment in otolaryngology. this study investigates the efficacy of the cleaning regimen for non-disposable instruments in an otolaryngology out-patients clinic. instruments were swabbed before each clinic, when they had been autoclaved, then after use on patients, when they had been washed with detergent and chlorhexi ...200312625885
endocarditis due to acinetobacter lwoffi on native mitral valve.endocarditis due to acinetobacter is a rare pathology with high mortality, reported mainly in hospitalized patients with predisposing risk factors. this is the second case of endocarditis due to acinetobacter reported in our country in the last 10 years.199910362380
evaluation of autoscan-w/a and the vitek gni+ automicrobic system for identification of non-glucose-fermenting gram-negative bacilli.the autoscan-w/a (w/a; dade behring microscan inc., west sacramento, calif.) and vitek automicrobic system (vitek ams; biomérieux vitek systems, inc., hazelwood, mo.) are both fully automated microbiology systems. we evaluated the accuracy of these two systems in identifying nonglucose-fermenting gram-negative bacilli. we used the w/a with conventional-panel neg combo type 12 and vitek gni+ identification systems. a total of 301 isolates from 25 different species were tested. of these, 299 isola ...200010699007
psychrobacters and related bacteria in freshwater fish.three phenotypic identification systems were employed to identify 106 strains of gram-negative, nonmotile, aerobic bacteria obtained during iced storage of wild (salmo trutta and esox lucius) and farmed (oncorhynchus mykiss) freshwater fish. using diagnostic tables and computer-assisted identification, the isolates were psychrobacter (64 strains), acinetobacter (24 strains), moraxella (6 strains), chryseobacterium (5 strains), myroides odoratus (2 strains), flavobacterium (1 strain), empedobacte ...200010716558
bactericidal and bacteriostatic activity of gemifloxacin against acinetobacter spp. in vitro.this study compared the in vitro bacteriostatic activity of gemifloxacin (sb-265805) and a panel of test antimicrobial agents against 100 clinical isolates of acinetobacter spp. (47 acinetobacter baumannii, 18 acinetobacter anitratus, 18 acinetobacter lwoffii, 13 acinetobacter calcoaceticus and four other acinetobacter spp.). gemifloxacin (mic(50/90) 0.06/16 mg/l) was more than eight-fold more potent than ciprofloxacin (0.5/>128 mg/l), two- to eight-fold more potent than grepafloxacin, moxifloxa ...200010824036
community-acquired pneumonia due to acinetobacter lwoffii in a patient infected with the human immunodeficiency virus. 19957727663
endotoxin in the occupational environment of bakers: method of detection.the present study was conducted in a large mechanical city bakery. the as-50 aspirators equipped with cellulose membrane filters were used for dust sampling. airborne microbial content was assessed by means of sedimentation and aspiration using an andersen sampler on petri plates containing mcconkey's medium. the following gram-negative rods were detected in the bakery atmosphere: erwinia herbicola, acinetobacter lwoffi and klebsiella oxytoca, in concentrations ranging from 1.4 10(4) to 3.5 10(5 ...19947820386
[pathogenic or saprophytic bacteria transforming glucose to gluconic acid, bacterium anitratum, b5w, moraxella lwoffi var. glucidolytica, neisseria winogradskyi]. 195413171653
[case of cerebrospinal meningitis from moraxella lwoffi in viet nam]. 195413182498
[proteolytic types of moraxella lwoffi and moraxella glucidolytica (bacterium anitratum)]. 195613327511
[septicemic state due to moraxella lwoffi]. 195613335885
complement deficiency predisposes for meningitis due to nongroupable meningococci and neisseria-related bacteria.nongroupable meningococci or bacteria related to the genus neisseria rarely cause meningitis. complement deficiency has been identified as a major predisposing factor for meningococcal disease. to assess whether patients with meningitis due to such strains have a complement deficiency, we studied 12 persons. six patients had meningitis due to nongroupable strains of meningococci, and six patients had meningitis due to moraxella species or acinetobacter species. inherited complement component c7 ...19948075270
engineering bacterial biopolymers for the biosorption of heavy metals; new products and novel formulations.bioremediation of heavy metal pollution remains a major challenge in environmental biotechnology. one of the approaches considered for application involves biosorption either to biomass or to isolated biopolymers. many bacterial polysaccharides have been shown to bind heavy metals with varying degrees of specificity and affinity. while various approaches have been adopted to generate polysaccharide variants altered in both structure and activity, metal biosorption has not been examined. polymer ...200011092618
[pathogenic properties of moraxella lwoffi]. 195713471085
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