| specific detection of campylobacter jejuni and campylobacter coli by using polymerase chain reaction. | development of a routine detection assay for campylobacter jejuni and campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction (pcr). an oligonucleotide primer pair from a conserved 5' region of the flaa gene of c. coli vc167 was used to amplify a 450-bp region by pcr. the primer pair specifically detected 4 strains of c. coli and 47 strains of c. jejuni; but it did not detect strains of campylobacter fetus, campylobacter lari, campylobacter upsaliensis, cam ... | 1992 | 1400961 |
| detection of campylobacter upsaliensis in diarrheic dogs and cats, using a selective medium with cefoperazone. | using a newly formulated selective medium containing cefoperazone, we isolated 72 campylobacter strains in fecal samples from 397 diarrheic dogs and cats. of these, 39 were thermophilic catalase-negative campylobacter species. we identified these campylobacter strains by dna:dna hybridization, using digoxigenin-labeled total genomic dna of 4 campylobacter reference strains (c jejuni, c coli, c lari, and c upsaliensis) as a probe. the labeling was done with a commercially available kit. we could ... | 1992 | 1539914 |
| campylobacter upsaliensis isolated from a breast abscess. | campylobacter upsaliensis is a rare human pathogen recovered so far only from stools or blood from patients with enterocolitis or bacteremia. we report the isolation of c. upsaliensis from a breast abscess. | 1992 | 1583149 |
| plasmid profiles of "campylobacter upsaliensis" isolated from blood cultures and stools of paediatric patients. | seventy-three clinical isolates of "campylobacter upsaliensis" were screened for the presence of plasmids. plasmid bands were found in 68 (93%) isolates, from which 14 plasmid types were identified. type 5 was found only in blood-culture isolates, whereas types 7-14 were found only in faecal isolates. plasmid-free isolates and the other plasmid profiles were present in both faecal and blood isolates. the reproducibility of these profiles was largely dependent on the method of plasmid isolation. ... | 1992 | 1625321 |
| campylobacter upsaliensis enteritis associated with canine infections. | | 1991 | 1675356 |
| revision of campylobacter, helicobacter, and wolinella taxonomy: emendation of generic descriptions and proposal of arcobacter gen. nov. | hybridization experiments were carried out between dnas from more than 70 strains of campylobacter spp. and related taxa and either 3h-labeled 23s rrnas from reference strains belonging to campylobacter fetus, campylobacter concisus, campylobacter sputorum, campylobacter coli, and campylobacter nitrofigilis, an unnamed campylobacter sp. strain, and a wolinella succinogenes strain or 3h- or 14c-labeled 23s rrnas from 13 gram-negative reference strains. an immunotyping analysis of 130 antigens ver ... | 1991 | 1704793 |
| is "campylobacter upsaliensis" an unrecognised cause of human diarrhoea? | for 3 years a filtration system for the isolation of "new" campylobacter was included in the culture protocol of 15,185 stool specimens. "c upsaliensis" was isolated in 99 patients, c jejuni subsp doylei in 4, and c hyointestinalis in 2. "c upsaliensis" was the only organism isolated in 83 patients. clinical information was available for 77 out of these 83 patients. 92% of the patients had diarrhoea; vomiting and fever were rare (14% and 7%, respectively); the onset was mostly sudden; and the sy ... | 1990 | 1968583 |
| isolation of group 2 aerotolerant campylobacter species from thai children with diarrhea. | campylobacter species were isolated from 93 (15%) of 631 thai children with diarrhea using the membrane filter technique on nonselective blood agar incubated at 37 degrees c. campylobacter jejuni was isolated from 62 (10%), campylobacter coli from 14 (2%), and atypical campylobacters from 17 (3%). the 17 atypical strains were first characterized biochemically and by dot blot dna hybridization. catalase-negative strains also were characterized by dna hybridization and ribotype pattern. one strain ... | 1991 | 2019754 |
| [evaluation of a latex agglutination method for the rapid identification of campylobacter jejuni/coli]. | a latex agglutination assay was developed to identify campylobacter jejuni and campylobacter coli. we evaluated the specificity, reproducibility and utility of the assay for clinical use and the following results were obtained. 1) to prepare standardized antigen, bacterial cells must be suspended to a density of 1 to 5 mcfarland unit, and heated at 121 degrees c for 10 to 30 min. 2) bacterial cells may be suspended either in the solution provided with the kit, or in physiological saline, without ... | 1990 | 2082036 |
| in vitro susceptibility of "campylobacter upsaliensis" to twenty-four antimicrobial agents. | the in vitro susceptibility of 41 strains of "campylobacter upsaliensis" to 24 antimicrobial agents was determined using a broth microdilution procedure. most isolates were susceptible to the fluoroquinolones and beta-lactam antibiotics tested, but all strains were resistant to trimethoprim (mbcs greater than or equal to 128 micrograms/ml) and teicoplanin (mbcs greater than or equal to 32 micrograms/ml). these agents may be useful in a selective isolation medium for "campylobacter upsaliensis". | 1990 | 2150816 |
| "campylobacter upsaliensis" sepsis in a boy with acquired hypogammaglobulinemia. | a 16-year-old boy with lupus erythematosus and hypogammaglobulinemia developed bacteremia with "campylobacter upsalinesis," a recently described campylobacter species with minimal catalase activity. because "c. upsaliensis" gram stains poorly and because it is susceptible to antibiotics in standard selective media, it may be overlooked in routine handling of blood cultures. | 1990 | 2282777 |
| characterization and description of "campylobacter upsaliensis" isolated from human feces. | during a 3-year period, "campylobacter upsaliensis" was isolated from 99 patients. phenotypic characterization and numerical analysis of protein electrophoregrams showed evidence that "c. upsaliensis" is a distinct campylobacter species with unique characteristics. the mbcs of 13 antibiotics were determined. in general, these organisms were highly susceptible to drugs that were present in the selective isolation media, making none of the available selective media suitable for the isolation of "c ... | 1990 | 2351720 |
| occurrence of plasmids in "campylobacter upsaliensis" (catalase negative or weak group) from geographically diverse patients with gastroenteritis or bacteraemia. | the natural occurrence of plasmids in "campylobacter upsaliensis" (catalase negative or weak campylobacters: cnw group) was investigated. the 62 strains isolated from patients with gastroenteritis or bacteraemia in five countries (south africa, belgium, sweden, uk and australia) were screened for plasmid dna, which was detected in 54 (87%) of them. plasmids were of various sizes between 1.4 and 90-md and up to three or possibly four plasmids were present in 26 (42%) strains. a total of 15 differ ... | 1990 | 2361534 |
| "campylobacter upsaliensis" isolated from cats as identified by dna relatedness and biochemical features. | campylobacter spp. were isolated from the feces of 20 (58%) of 53 asymptomatic cats during routine physical examination while the cats were maintained in an accredited quarantine facility. fifteen of these campylobacter spp. were identified phenotypically as campylobacter jejuni, and two were identified as c. coli. dna-dna hybridization (hydroxyapatite method) was used to confirm the identification of three thermotolerant catalase-negative isolates. they were 80 to 100% related to each other and ... | 1989 | 2584385 |
| "campylobacter upsaliensis" isolated from blood cultures of pediatric patients. | seventeen campylobacters isolated from cultures of blood samples of 16 bacteremic patients were susceptible to cephalothin and were either catalase negative or had only weak catalase activity (cnw strains) and were classified as "campylobacter upsaliensis" (k. sandstedt and j. ursing, xiv int. congr. microbiol., p. b8-17, 1986). all of the patients had predisposing conditions, and 10 patients were less than or equal to 12 months old, indicating that "c. upsaliensis" is an opportunistic pathogen ... | 1989 | 2723034 |
| direct isolation of atypical thermophilic campylobacter species from human feces on selective agar medium. | campylobacter upsaliensis is the name which has been proposed for a new group of thermophilic campylobacter strains which differ from c. jejuni and c. coli in having a negative or weak catalase reaction. primary isolation of these strains from human feces has been achieved only by use of filtration techniques. we report here direct isolation of strains corresponding to c. upsaliensis from stools of six children. the strains were isolated on a newly described campylobacter-selective medium. the s ... | 1989 | 2723035 |
| isolation and characterization of catalase-negative and catalase-weak strains of campylobacter species, including "campylobacter upsaliensis," from humans with gastroenteritis. | during 1987 and 1988, nine strains of catalase-negative or -weak campylobacter species were isolated in alberta, canada. dna hybridization studies demonstrated that seven strains were "campylobacter upsaliensis," one strain was highly homologous with campylobacter jejuni dna, and one strain was a campylobacter unrelated to the other two species. all "c. upsaliensis" strains were hippurate negative, and six of seven were susceptible to cephalothin. the unusual variant of c. jejuni was hippurate p ... | 1989 | 2778067 |
| isolation of campylobacter upsaliensis from stool specimens. | | 1989 | 2778081 |
| human disease associated with "campylobacter upsaliensis" (catalase-negative or weakly positive campylobacter species) in the united states. | catalase-negative or weakly positive (cnw) thermotolerant campylobacteria, first isolated from dogs in 1983, were recently recognized as a new species, "campylobacter upsaliensis," but their association with human illness has not been established. twelve human isolates received at the centers for disease control between 1980 and 1986 were identified as cnw campylobacteria by biochemical tests, cellular fatty acid composition, and antimicrobial susceptibility patterns. eleven cnw campylobacter st ... | 1989 | 2913038 |
| discrimination among thermophilic campylobacter species by polymerase chain reaction amplification of 23s rrna gene fragments. | by comparing nucleic acid sequences determined for one of the most variable areas of 23s rrna genes of 23 campylobacter strains, we were able to identify regions specific for thermophilic campylobacter strains. oligonucleotide primers corresponding to these unique regions were synthesized and used in the polymerase chain reaction. one primer pair selectively detected all thermophilic campylobacter species, while four other primer pairs allowed discrimination among the thermophilic species campyl ... | 1993 | 7508460 |
| distinct genotypes of human and canine isolates of campylobacter upsaliensis determined by 16s rrna gene typing and plasmid profiling. | the utility of combined 16s rrna (rrs) gene restriction fragment length polymorphism and plasmid profiles to differentiate between and within campylobacter upsaliensis of human and canine origin was examined. fourteen distinct rrs gene restriction fragment length polymorphs consisting of bands sized between 1.9 and 4.8 kb were observed. the copy number of the 16s rrna gene was three in most strains of c. upsaliensis. plasmids were found in almost 60% of the strains; ranging in size from 1.5 to 1 ... | 1994 | 7523440 |
| physical and genetic map of the genome of campylobacter upsaliensis. | a physical map of campylobacter upsaliensis atcc 43954 was constructed from dna fragments generated by sali (5' g/tcgac), nari (5' gg/cgcc) and bsshii (5' g/cgcgc) restriction digests separated using pulsed-field gel electrophoresis. the size of the c. upsaliensis genome was approximately 2000 kb, providing evidence of the largest campylobacter genome sized to date. twenty-one fragments created from these restriction digests were assembled into a physical map using a combination of complementary ... | 1995 | 7582001 |
| investigation of an outbreak of campylobacter upsaliensis in day care centers in brussels: analysis of relationships among isolates by phenotypic and genotypic typing methods. | an outbreak of campylobacter upsaliensis in four brussels day care centers (a, b-1, b-2, and c) affected 44 children. diarrhea was the major symptom. from january 1991 to june 1992, the outbreak strain was isolated from 3, 1, and 21 (of 68) children in centers a, b-1, and b-2, respectively, and from 19 of 22 children in center c, igg, igm, and iga antibodies were detected by western blotting of serum specimens of 9 of 10 and 13 of 16 children in centers b-2 and c, respectively. strains were type ... | 1995 | 7594667 |
| campylobacter upsaliensis, c. sputorum sputorum and c. concisus as common causes of diarrhoea in swedish children. | | 1995 | 7660089 |
| culture media for the isolation of campylobacters. | the history of the development of selective media for isolation of campylobacters, including the rationale for choice of selective agents is described. developments have included modifications to allow incubation at 37 degrees c instead of 42 or 43 degrees c and changes in the types and concentrations of antibiotics in order not to inhibit organisms such as campylobacter upsaliensis, c. jejuni subsp. doylei and some strains of c. coli and c. lari. when examining foods, plating media originally d ... | 1995 | 7662519 |
| detection of campylobacter upsaliensis from a blood culture by using the bact/alert system. | campylobacter upsaliensis was isolated from the blood of a 60-year-old female with hairy cell leukemia. this spiral-shaped organism was detected in the aerobic bact/alert bottle (organon teknika, durham, n.c.) by acridine orange staining and was recovered only on chocolate agar in a microaerophilic atmosphere at 35 degrees c. | 1994 | 7814508 |
| abortion associated with campylobacter upsaliensis. | campylobacter upsaliensis was isolated from the blood and fetoplacental material of an 18-week-pregnant woman who had contact with a household cat. we believe this is the first report of abortion associated with c. upsaliensis infection. | 1994 | 7883913 |
| campylobacter upsaliensis bacteraemia in an aboriginal child. | | 1994 | 8177117 |
| selective medium for thermophilic campylobacters including campylobacter upsaliensis. | to develop a selective supplement for the recovery of thermophilic campylobacter spp, including campylobacter upsaliensis from faeces, using campylobacter blood free selective agar base as the growth medium. | 1993 | 8227433 |
| occurrence of campylobacters in small domestic and laboratory animals. | faeces samples from 156 healthy domestic pets and laboratory animals were examined for campylobacters with both a selective medium (campylobacter blood free medium; oxoid, cm739) and selective filtration through a 0.65 micron pore size filter. campylobacter spp. were isolated from 78 of the samples; filtration was the most effective method. isolates were characterized by biochemical tests and dna/dna hybridization with whole chromosomal dna from reference strains as probes. campylobacter upsalie ... | 1993 | 8365954 |
| a comparison of a new campylobacter selective medium (cat) with membrane filtration for the isolation of thermophilic campylobacters including campylobacter upsaliensis. | the newly developed cat campylobacter selective medium employing the blood-free charcoal-based agar containing cefoperazone (8 mg l-1), amphotericin (10 mg l-1) and teicoplanin (4 mg l-1) was compared with the membrane filtration culture technique for isolation of campylobacter spp. including camp. upsaliensis. nine hundred and fifty human, 275 dog and 65 cat faeces (in which modified ccda medium was also compared) were tested. in addition, the recovery of camp. upsaliensis from pure cultures an ... | 1996 | 8698666 |
| pulsed-field gel electrophoresis indicates genotypic heterogeneity among campylobacter upsaliensis strains. | to determine the genomic relatedness among a selection of animal and human campylobacter upsaliensis isolates, macrorestriction profiles were generated for 20 c. upsaliensis strains, among 7 serogroups, using pulsed-field gel electrophoresis (pfge). xhoi, sali and sacii restriction enzyme profiles indicated genomic heterogeneity among strains. using xhoi and sacii restriction enzyme digestion, genomic similarities between some pairs of strains were lior serogroup specific. the genomic sizes of t ... | 1996 | 8807802 |
| hemolytic-uremic syndrome associated with acute campylobacter upsaliensis gastroenteritis. | | 1996 | 8893206 |
| adherence to lipids and intestinal mucin by a recently recognized human pathogen, campylobacter upsaliensis. | campylobacter upsaliensis is a recently recognized human enteric pathogen associated with enteritis, colitis, bacteremia, and sepsis. very little is known about the mechanisms of pathogenesis of this organism. the goals of this study were to determine whether c. upsaliensis binds to epithelial cells and whether there are specific lipid molecules that might serve as cell membrane receptors. in addition, we also explored c. upsaliensis binding to purified human small-intestinal mucin, since the mu ... | 1996 | 8926069 |
| characterization of campylobacter upsaliensis fur and its localization in a highly conserved region of the campylobacter genome. | despite increasing recognition of the importance of campylobacter upsaliensis in human disease little is known about either the virulence properties or genetics of this enteric pathogen. the complete coding sequence of a c. upsaliensis gene has yet to be published. we have cloned and sequenced the complete iron-uptake regulatory (fur) gene from the type strain of this species. the c. upsaliensis fur homolog was isolated from a genomic library of c. upsaliensis atcc 43954 constructed in phage lam ... | 1996 | 8996110 |
| motor nerve terminal degeneration provides a potential mechanism for rapid recovery in acute motor axonal neuropathy after campylobacter infection. | we investigated the possible mechanisms of paralysis and recovery in a patient with the acute motor axonal neuropathy (aman) pattern of the guillain-barré syndrome. the aman pattern of gbs is characterized clinically by acute paralysis without sensory involvement and electrodiagnostically by low compound motor action potential amplitudes, suggesting axonal damage, without evidence of demyelination. many aman patients have serologic or culture evidence of recent campylobacter jejuni infection. pa ... | 1997 | 9065554 |
| molecular discrimination between campylobacter jejuni, campylobacter coli, campylobacter lari and campylobacter upsaliensis by polymerase chain reaction based on a novel putative gtpase gene. | polymerase chain reaction (pcr) mediated dna fingerprinting has resulted in the identification of a novel campylobacter jejuni gene, encoding a gtpase protein. the gene, consisting of 383 amino acids contained semi-conserved gtp-binding sites (designated g-1 to g-4), that are characteristic for members of the gtpase protein superfamily. remarkably, this gene from c. jejuni appears to encode a member of a novel family of gtp-binding proteins, containing two separate putative gtp-binding domains, ... | 1997 | 9232616 |
| polymerase chain reaction detection and speciation of campylobacter upsaliensis and c. helveticus in human faeces and comparison with culture techniques. | a polymerase chain reaction (pcr) assay based on the 16s rrna gene and an improved dna extraction procedure were developed for the direct detection and differentiation of campylobacter upsaliensis and c. helveticus in seeded human faeces. the pcr assay was compared with culture detection by a membrane filter (mf) technique and on selective agar (sa) containing 8 mg l-1 cefoperazone. both mf culture and the pcr assay detected 10(5) colony-forming units (cfu) g-1 faeces. selective agar culture of ... | 1997 | 9351218 |
| healthy puppies and kittens as carriers of campylobacter spp., with special reference to campylobacter upsaliensis. | living in a household with a dog or cat has previously been identified as a significant risk factor for acquiring campylobacteriosis, in particular, with reference to campylobacter upsaliensis infection. in a cross-sectional study carried out in denmark between august and december 1996, 72 healthy puppies and 42 healthy kittens, aged between 11 and 17 weeks, were sampled for fecal campylobacter shedding by culture of rectal swab specimens on blood-free agar base with cefoperazone at 32 mg/liter ... | 1997 | 9399557 |
| comparison of the productivity of cefoperazone amphotericin teicoplanin (cat) agar and modified charcoal cefoperazone deoxycholate (mccd) agar for various strains of campylobacter, arcobacter and helicobacter pullorum. | cefoperazone amphotericin teicoplanin (cat) agar was developed from cefoperazone deoxycholate (mccd) agar by modification of the selective antibiotics in order to permit growth of strains of campylobacter upsaliensis. in this study, 35 strains of campylobacter and arcobacter were tested for their ability to grow on cat and mccd media using the ecometric method. six of these strains were also tested using the modified miles-misra method. overall, nineteen strains out of the 35 tested grew better ... | 1997 | 9506285 |
| campylobacter upsaliensis: waiting in the wings. | despite strong epidemiological evidence supporting an important role for campylobacter upsaliensis as a human enteropathogen, it remains relatively unknown in the realm of clinical microbiology. clinical studies indicate that infection with this organism usually is associated with benign self-limiting diarrhea. however, more serious illnesses, including spontaneous abortion and hemolytic-uremic syndrome, recently have been associated with human infections. understanding of the virulence properti ... | 1998 | 9665977 |
| [campylobacter upsaliensis: first report of its clinical isolation and identification of a reservoir in chile]. | | 1998 | 9674307 |
| [clinical decision making in family practice. a patient with abdominal pain and chills]. | an 82-year-old man was admitted because of abdominal pain and a shaking chill. his medical history revealed ileocecal resection because of ileitis associated with a yersinia infection 3 years before admission. one year later he was readmitted because of bowel obstruction due to recurrent ileitis. he was treated with trimethoprim-sulfamethoxazole for two weeks because of positive serological tests for yersinia and made a full recovery except for chronic diarrhoea. on the current admission, stool ... | 1998 | 9752068 |
| use of a selective medium and a membrane filter method for isolation of campylobacter species from spanish paediatric patients. | a study was conducted to assess the value of a combination of two culture methods for isolation of campylobacter spp. from spanish children. seven hundred twenty-nine diarrhoeal stool specimens from 599 patients were examined for campylobacter spp. by culturing them on charcoal cefoperazone deoxycholate agar and on blood agar with a membrane filter. one hundred sixteen campylobacter strains were isolated from a total of 108 specimens; 75 (64.6%) were campylobacter jejuni, 32 (27.5%) were campylo ... | 1998 | 9764551 |
| a reverse transcriptase polymerase chain reaction assay for the detection of thermophilic campylobacter spp. | a novel method was developed for the detection of thermophilic enteropathogenic campylobacters based on the detection of mrna using the reverse transcriptase polymerase chain reaction (rt-pcr). the rna extraction method, dnase treatment and rt-pcr assay were shown to be specific for mrna. the assay is specific for the thermophilic campylobacters campylobacter jejuni, campylobacter coli and campylobacter upsaliensis and restriction fragment length polymorphism (rflp) analysis of the 256 bp amplif ... | 1998 | 9778457 |
| campylobacter upsaliensis-associated diarrhea in human immunodeficiency virus-infected patients. | campylobacter upsaliensis was isolated from the feces of 20 human immunodeficiency virus (hiv)-infected patients with diarrhea over a 67-month period, representing 18.5% of fecal campylobacter isolates from our hiv-seropositive patients. all isolates were recovered from feces by a 0.45-micron-membrane filtration method. culture on skirrow's selective medium incubated at 42 degrees c failed to support any growth. the mean cd4 cell count of the patient group was 185/mm3 (range, 0-840/mm3), and all ... | 1998 | 9798039 |
| differentiation of campylobacter coli and c. jejuni by length and dna sequence of the 16s-23s rrna internal spacer region. | the internal spacer region (isr) between the 16s and 23s rrna genes of campylobacter was investigated by pcr fragment length typing and dna sequencing of clinical and chicken wild-type isolates. pcr fragment length typing showed one fragment of 859 nt in length for the 12 strains of campylobacter coli investigated. thirty-six of the campylobacter jejuni subsp. jejuni strains possessed one fragment, which varied in size between 727 and 802 nt. three strains showed two fragments between 501 and 92 ... | 1999 | 10206715 |
| are campylobacter curvus and campylobacter upsaliensis antecedent infectious agents in guillain-barré and fisher's syndromes? | campylobacter curvus and campylobacter upsaliensis were isolated from stools of patients with guillain-barré (gbs) or fisher's (fs) syndromes. whether these microorganisms are pathogens of antecedent diarrhea in gbs and fs is not clear, therefore, we made a serological examination. there were no differences in antibody titer to these organisms among the patients with gbs, fs, and the controls. some patients had elevated antibodies to the bacteria, but most also had serological evidence of c. jej ... | 1999 | 10223411 |
| rapid identification of thermotolerant campylobacter jejuni, campylobacter coli, campylobacter lari, and campylobacter upsaliensis from various geographic locations by a gtpase-based pcr-reverse hybridization assay. | recently, a gene from campylobacter jejuni encoding a putative gtpase was identified. based on two semiconserved gtp-binding sites encoded within this gene, pcr primers were selected that allow amplification of a 153-bp fragment from c. jejuni, c. coli, c. lari, and c. upsaliensis. sequence analysis of these pcr products revealed consistent interspecies variation, which allowed the definition of species-specific probes for each of the four thermotolerant campylobacter species. multiple probes we ... | 1999 | 10325325 |
| isolation and purification of a campylobacter upsaliensis autolysin. | autolytic activity in the soluble and sediment fractions of sonicates of the spiral and the coccoid form of campylobacter upsaliensis could not be demonstrated by native (nondenaturing) polyacrylamide gel electrophoresis (page). autolysins were detected, however, by using denaturing sodium dodecyl sulfate (sds)-page gels containing either purified escherichia coli peptidoglycan or whole cells of micrococcus luteus (micrococcus lysodeikticus) as the turbid substrate, with subsequent renaturation ... | 1999 | 10349717 |
| campylobacter upsaliensis gastroenteritis in childhood. | campylobacter upsaliensis can cause gastroenteritis and bacteremia. data on its epidemiology and role in pediatric gastroenteritis are limited. | 1999 | 10571436 |
| campylobacter species in cats and dogs in south australia. | campylobacter enteritis was the most frequently notified infectious disease in australia in 1996 and campylobacter species have been associated with extra-intestinal infections such as purulent arthritis and guillian-barré syndrome. dogs and cats are known to carry campylobacteria and contact with household pets have been implicated as possible sources of human infection. | 1999 | 10590795 |
| rapid detection of campylobacter jejuni in stool specimens by an enzyme immunoassay and surveillance for campylobacter upsaliensis in the greater salt lake city area. | the alexon-trend, inc. (ramsey, minn.), prospect campylobacter microplate assay was compared with culture on a campy-cva plate (remel, lenexa, kans.) and blood-free campylobacter agar with cefoperazone (20 microg/ml), amphotericin b (10 microg/ml), and teicoplanin (4 microg/ml) (cat medium; oxoid limited, hampshire, england) with 631 patient stool samples. the cat medium was used to isolate campylobacter upsaliensis. the enzyme immunoassay (eia) had a sensitivity and a specificity of 89 and 99%, ... | 2000 | 10921981 |
| the incidence and pcr detection of campylobacter upsaliensis in dogs and cats. | a study was made of the incidence of campylobacter upsaliensis among dogs and cats suffering from acute or chronic diarrhoea; 225 dogs and cats were examined and 51 strains were identified, 16 (7%) of which were camp. upsaliensis. when rectal swabs were taken from a control group of 126 dogs and cats without clinical symptoms, 19 campylobacter spp. and four camp. upsaliensis were identified. all the camp. upsaliensis strains were isolated in dogs. the campylobacter strains were identified on the ... | 2000 | 10972730 |
| campylobacter upsaliensis exerts a cytolethal distending toxin effect on hela cells and t lymphocytes. | campylobacter upsaliensis is an emerging human enteropathogen. however, little is known about the pathogenesis of c. upsaliensis infection. in this study the authors demonstrate that c. upsaliensis whole-cell preparations and extracts produce a cytolethal distending toxin (cdt)-like effect on hela cells characterized by progressive distension and nuclear fragmentation culminating in cell death over 5 d. to further delineate the nature of this toxic effect in relation to cdt from other pathogens, ... | 2001 | 11238981 |
| identification of thermophilic campylobacter spp. by phenotypic and molecular methods. | the differences between phenotyping and genotyping (polymerase chain reaction- restriction fragment length polymorphism) of campylobacter jejuni, campylobacter coli, campylobacter lari and campylobacter upsaliensis were assessed. | 2001 | 11298244 |
| coinfection of enteric helicobacter spp. and campylobacter spp. in cats. | during a 6-year period, 64 of 227 commercially reared cats had microaerobic bacteria isolated from their feces. all the isolates were initially identified as campylobacter-like organisms based on biochemical and phenotypic characteristics. dna extractions from 51 of these isolates were subjected to pcr using primers specific for helicobacter spp. and campylobacter spp. of the isolates, 92% (47 of 51 isolates) were positive for campylobacter spp., 41% (21 of 51 isolates) were positive for helicob ... | 2001 | 11376052 |
| genomic heterogeneity and o-antigenic diversity of campylobacter upsaliensis and campylobacter helveticus strains isolated from dogs and cats in germany. | a serotyping scheme based on heat-stable surface antigens was established for 101 campylobacter upsaliensis and 10 campylobacter helveticus strains isolated from 261 dogs and 46 cats of different ages originating from two geographically distinct regions in germany. the prevalence of c. upsaliensis varied between 27.8% in juvenile dogs (<12 months of age) and 55.4% in adult dogs (p < 0.05). of the cats, 19.6% harbored c. upsaliensis, whereas 21.7% carried c. helveticus. of the c. upsaliensis isol ... | 2001 | 11427567 |
| basis of the superiority of cefoperazone amphotericin teicoplanin for isolating campylobacter upsaliensis from stools. | the optimum method for isolating campylobacter upsaliensis from stools has not been clearly defined. in a preliminary study, cefoperazone amphotericin teicoplanin (cat) selective medium isolated six c. upsaliensis strains which were not detected using modified cefoperazone charcoal deoxycholate (mccda). in order to identify the factors that underlie the superiority of cat over mccda for isolating c. upsaliensis, we examined the effect of incubation time and antibiotic content of culture media on ... | 2001 | 11427603 |
| differentiation of campylobacter species by aflp fingerprinting. | the fluorescent amplified fragment length polymorphism (aflp) fingerprinting method was tested for its ability to identify and subtype the most important campylobacter species found in veterinary infections. sixty-nine reference strains and 19 clinical isolates of campylobacter jejuni subsp. jejuni, campylobacter jejuni subsp. doylei, campylobacter upsaliensis, campylobacter coli, campylobacter lari, campylobacter fetus subsp. fetus, c. fetus subsp. venerealis, campylobacter hyointestinalis subs ... | 2001 | 11577152 |
| efficient isolation of campylobacter upsaliensis from stools. | | 2001 | 11712518 |
| guillain-barre syndrome and campylobacter infection. | campylobacter infection of the gastrointestinal tract has been observed as an antecedent illness in some patients with guillain-barre syndrome (gbs); these patients have been reported to have poor prognosis. we investigated 29 patients with gbs, admitted to our hospital from january 1996 to december 1999 for recent campylobacter enteritis by culture of their stool specimens. campylobacter upsaliensis and c. jejuni were isolated from stools of one patient each with acute motor axonal neuropathy ( ... | 2001 | 11944711 |
| successful treatment of prosthetic knee infection due to campylobacter upsaliensis. | | 2002 | 11957030 |
| campylobacter upsaliensis: another pathogen for consideration in the united states. | while evaluating quinolone resistance in a sample of campylobacter isolates recovered from patients with campylobacteriosis in los angeles county, california, in 1998, we discovered that the second most frequently isolated species was campylobacter upsaliensis (6 [4%] of 155 isolates). the ability of laboratories to recover this species may be dependent on the culture conditions and the media used. three dogs living in the households of 2 of these 6 patients had c. upsaliensis isolated in their ... | 2002 | 12015708 |
| isolation and characterization of campylobacter, helicobacter, and anaerobiospirillum strains from a puppy with bloody diarrhea. | we carried out a microscopic examination of stools from a 2-month-old female puppy with bloody diarrhea, and this revealed large numbers of different spiral-shaped bacteria. to isolate these organisms, a rectal swab specimen was inoculated onto plates of skirrow's agar and incubated at 37 degrees c for 6 days in a microaerobic atmosphere. finally, a total of six different spiral-shaped bacteria (strains g1104, 94105, fr106, b0101, 3j102, and j2103) were isolated. based on their morphology, bioch ... | 2002 | 12069772 |
| speciating campylobacter jejuni and campylobacter coli isolates from poultry and humans using six pcr-based assays. | six previously published polymerase chain reaction (pcr) assays each targeting different genes were used to speciate 116 isolates previously identified as campylobacter jejuni using routine microbiological techniques. of the 116 isolates, 84 were of poultry origin and 32 of human origin. the six pcr assays confirmed the species identities of 31 of 32 (97%) human isolates and 56 of 84 (67%) poultry isolates as c. jejuni. twenty eight of 84 (33%) poultry isolates were identified as campylobacter c ... | 2002 | 12435503 |
| invasion of human epithelial cells by campylobacter upsaliensis. | few data exist on the interaction of campylobacter upsaliensis with host cells, and the potential for this emerging enteropathogen to invade epithelial cells has not been explored. we have characterized the ability of c. upsaliensis to invade both cultured epithelial cell lines and primary human small intestinal cells. epithelial cell lines of intestinal origin appeared to be more susceptible to invasion than non-intestinal-derived cells. of three bacterial isolates studied, a human clinical iso ... | 2003 | 14531898 |
| isolation and identification of thermophilic campylobacter species in faecal samples from swedish dogs. | to investigate the role of swedish dogs as potential reservoirs of thermophilic campylobacter species, faecal samples were analysed from 91 dogs in 2001. the majority of dogs (n = 84) were healthy family dogs. campylobacter spp. were isolated from 51 of the 91 dogs (56%). a significant difference in isolation rates was observed between younger and older dogs: 76% of the younger dogs (5-12 months) were positive, compared with 39% of dogs > or = 13 months (p < 0.01). two different selective media, ... | 2003 | 14606609 |
| [campylobacteriosis in a low-income community in buenos aires, argentina]. | the authors describe the problem of campylobacteriosis in a low-income community located in southern buenos aires. homes in the area were classified according to their total number of inhabitants, the number of children and animals living in the house and the amount of poultry meat consumed. samples were taken from all the different types of homes that had been identified in order to isolate, identify and type campylobacter sp., using the modified skirrow technique, bio-chemical tests and the li ... | 2003 | 15005557 |
| campylobacter upsaliensis isolated from young dogs with and without diarrhea. | | 2004 | 15042192 |
| longitudinal study of the excretion patterns of thermophilic campylobacter spp. in young pet dogs in denmark. | the campylobacter excretion patterns of 26 domestic pet dogs were described in a longitudinal study. the dogs entered the study between 3 and 8 months of age and were monitored until 2 years of age. they were tested monthly for campylobacter carriage in stool samples that were cultured on the campylobacter-selective media cat and modified ccda agar at 37 and 42 degrees c. this study comprised 366 fecal swab samples, of which 278 (76.2%) were found to be campylobacter positive, with the following ... | 2004 | 15131162 |
| campylobacter infection in 682 bulgarian patients with acute enterocolitis, inflammatory bowel disease, and other chronic intestinal diseases. | the aim of the study was to assess campylobacter infections in 309 patients with acute enterocolitis, 272 patients with relapses of chronic enterocolitis, 70 patients with inflammatory bowel disease (involving crohn's disease and ulcerative colitis) and 31 patients with other chronic intestinal illnesses. isolation and identification were performed conventionally. limited agar dilution method was used for susceptibility testing of the strains. campylobacter species were isolated in patients with ... | 2004 | 15135505 |
| high-resolution genotyping of campylobacter upsaliensis strains originating from three continents. | ninety-six campylobacter upsaliensis strains that originated from australia, canada, and europe (germany) and that were isolated from humans, dogs, and cats were serotyped for their heat-stable surface antigens. all of them were genotyped by enterobacterial repetitive intergenic consensus sequence pcr (eric-pcr) profiling, and 83 strains were genotyped by macrorestriction analysis with the endonuclease xhoi. eighty-four percent of the strains belonged to five different serotypes (serotypes oi, o ... | 2004 | 15297481 |
| differentiation of campylobacter coli, campylobacter jejuni, campylobacter lari, and campylobacter upsaliensis by a multiplex pcr developed from the nucleotide sequence of the lipid a gene lpxa. | we describe a multiplex pcr assay to identify and discriminate between isolates of campylobacter coli, campylobacter jejuni, campylobacter lari, and campylobacter upsaliensis. the c. jejuni isolate f38011 lpxa gene, encoding a udp-n-acetylglucosamine acyltransferase, was identified by sequence analysis of an expression plasmid that restored wild-type lipopolysaccharide levels in escherichia coli strain sm105 [lpxa(ts)]. with oligonucleotide primers developed to the c. jejuni lpxa gene, nearly fu ... | 2004 | 15583280 |
| epidemiologic features of campylobacter infection among cats in the upper midwestern united states. | to describe the epidemiologic features of campylobacter infection among cats in the minneapolis-saint paul metropolitan area. | 2005 | 15742694 |
| development of real-time pcr and hybridization methods for detection and identification of thermophilic campylobacter spp. in pig faecal samples. | to develop a real-time (rt) pcr for species differentiation of thermophilic campylobacter and to develop a method for assessing co-colonization of pigs by campylobacter spp. | 2005 | 16033460 |
| sequence analysis of two cryptic plasmids from an agricultural isolate of campylobacter coli. | as part of a study identifying plasmids in campylobacter, we isolated and sequenced two novel cryptic plasmids from an agricultural isolate of campylobacter coli. the larger of the two plasmids, p3384, is 3316 bp in length and has a g+c content of 31.18%. a typical origin of replication consisting of five iterons was observed directly upstream of the first of three putative orfs. the smaller plasmid, p3386, is 2426 bp in length and has a g+c content of 26.22%. of the three putative orfs detected ... | 2006 | 16120460 |
| speciation of campylobacter coli, c. jejuni, c. helveticus, c. lari, c. sputorum, and c. upsaliensis by matrix-assisted laser desorption ionization-time of flight mass spectrometry. | multiple strains of campylobacter coli, c. jejuni, c. helveticus, c. lari, c. sputorum, and c. upsaliensis isolated from animal, clinical, or food samples have been analyzed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms). whole bacterial cells were harvested from colonies or confluent growth on agar and transferred directly into solvent and then to a spot of dried 3-methoxy-4-hydroxycinnamic acid (matrix). multiple ions in the 5,000- to 15,000-da m ... | 2005 | 16204551 |
| antimicrobial susceptibility of clinical isolates of non-jejuni/coli campylobacters and arcobacters from belgium. | to determine the susceptibility of non-jejuni/coli campylobacters and arcobacters isolated from diarrhoeal stool specimens in belgium. | 2006 | 16533825 |
| acridine orange staining and viability of the coccoid form of campylobacter upsaliensis. | conversion of campylobacter upsaliensis to the coccoid form during aerobic incubation at 37 degrees c was not prevented by treatment with chloramphenicol and was accompanied by severe decreases in isocitrate dehydrogenase activity and oxygen uptake. although the coccoid forms fluoresced orange-red by acridine orange staining, agarose gel electrophoresis indicated an extensive degradation of the ribosomal rna. this suggests that acridine orange staining may not be a good indicator of viability an ... | 2005 | 16858932 |
| campylobacter insulaenigrae isolates from northern elephant seals (mirounga angustirostris) in california. | there are only two reports in the literature demonstrating the presence of campylobacter spp. in marine mammals. one report describes the isolation of a new species, campylobacter insulaenigrae sp. nov., from three harbor seals (phoca vitulina) and a harbor porpoise (phocoena phocoena) in scotland, and the other describes the isolation of campylobacter jejuni, campylobacter lari, and an unknown campylobacter species from northern elephant seals (mirounga angustirostris) in california. in this st ... | 2007 | 17259365 |
| distribution and genetic variability among campylobacter spp. isolates from different animal species and humans in switzerland. | in switzerland, a national database with 1028 campylobacter isolates from poultry, pigs, cats, dogs, cattle, humans, zoo animals and water has been created. the database contains the genetic fingerprint and background information of each campylobacter isolate. dominant species could be identified in the different sources with a majority of campylobacter jejuni in poultry (73%), humans (79%), cattle (95%), zoo animals (40%) and water (100%), of campylobacter coli in pigs (72%), and of campylobact ... | 2007 | 17359440 |
| amino acid sequence determination of protein biomarkers of campylobacter upsaliensis and c. helveticus by "composite" sequence proteomic analysis. | we have identified the protein biomarkers observed in the matrix-assisted laser desorption/ionization time-of-flight mass spectra (maldi-tof-ms) of cell lysates of five strains of campylobacter upsaliensis and one strain of c. helveticus by "bottom-up" proteomic techniques. only one c. upsaliensis strain had previously been genomically sequenced. the significant findings are as follows: (1) the protein biomarkers identified were: 10 kd chaperonin, protein of unknown function (duf465), phna prote ... | 2007 | 17508732 |
| development of a multiplex pcr assay for identification of campylobacter coli, campylobacter fetus, campylobacter hyointestinalis subsp. hyointestinalis, campylobacter jejuni, campylobacter lari and campylobacter upsaliensis. | a multiplex pcr assay has been developed for the identification of the six common campylobacter taxa associated with human gastroenteritis and/or septicaemia, namely campylobacter coli, campylobacter fetus, campylobacter hyointestinalis subsp. hyointestinalis, campylobacter jejuni, campylobacter lari and campylobacter upsaliensis. the assay was developed using a combination of newly designed and published primers. it provided a specific pcr product for each of the five campylobacter species and ... | 2007 | 17965346 |
| comparative analysis of human and canine campylobacter upsaliensis isolates by amplified fragment length polymorphism. | human (n = 33) and canine (n = 53) campylobacter upsaliensis isolates from seven countries were genotyped by a new amplified fragment length polymorphism method. we observed 100% typeability and high overall diversity. the majority of human strains (23/33) clustered separately from canine strains, indicating that dogs may not be the main source of human infection. | 2008 | 18287320 |
| prevalence and antimicrobial resistance of pathogenic bacteria in chicken and guinea fowl. | this study was conducted to compare the presence and antimicrobial susceptibility of campylobacter, salmonella spp., and other enteric bacteria between chickens and guinea fowls. birds were reared on enclosed concrete floor housing covered with pine wood shavings litter material. chicken (n = 40) and guinea fowl (n = 40) carcasses, drinking water (10 ml; n = 40), and litter (10 g; n = 40) were aseptically collected randomly from a poultry farm and analyzed within 1 h of collection. individual pe ... | 2008 | 18753453 |
| a novel method and simple apparatus for the detection of thermophilic campylobacter spp. in chicken meat products. | conventional procedures for isolation of thermophilic campylobacter spp. from chicken are complex, labor intensive, and time-consuming. the objective of this study was to create a novel campylobacter culturing apparatus. a main concept of the device was based on the ability of campylobacter to pass through a 0.45 microm pore size filter in viscous media. preliminary study demonstrated that only viable campylobacter moved through the membrane filter and could multiply in the enrichment culture. c ... | 2009 | 18992776 |
| prevalence of thermophilic campylobacter species in household cats and dogs in ireland. | rectal swabs were collected from 147 household dogs and 35 household cats, including healthy animals, animals with gastrointestinal signs and animals with a variety of medical and surgical conditions. a combination of selective culture methods was used to optimise the recovery of campylobacter species, and a pcr was used to confirm their isolation and to identify the species. the overall prevalence of campylobacter species was 42.9 per cent in the cats and 41.5 per cent in the dogs. campylobacte ... | 2009 | 19136683 |
| occurrence and characterization of campylobacter in the brazilian production and processing of broilers. | twenty-two commercial broiler flocks and their carcasses, totaling 546 samples (450 collected from a poultry farm and 96 from a slaughterhouse), were surveyed for the presence of campylobacter. the positive results for campylobacter among the analyzed samples were homogeneous, yielding 81.8% for cecal droppings, 80.9% for feces, and 80.4% for cloacal swabs. pre-enrichment and direct plating showed that 77.85% and 81.8% of cloacal swabs, respectively, were positive for campylobacter compared to 9 ... | 2008 | 19166063 |
| prevalence of campylobacter spp. in a cross-sectional study of dogs attending veterinary practices in the uk and risk indicators associated with shedding. | campylobacteriosis is a major cause of gastroenteritis in humans and some studies have suggested that dog ownership is a risk factor for the condition. to determine the prevalence, species distribution, and risk indicators for campylobacter spp. infecting dogs attending veterinary practices in uk, faecal samples were collected in a cross-sectional study from 249 dogs with and without clinical signs. the prevalence of campylobacter spp. was 38% (95% ci 32, 44), with campylobacter upsaliensis acco ... | 2010 | 19223212 |
| risk factors for the carriage of campylobacter upsaliensis by dogs in a community in cheshire. | samples of faeces were taken from 183 healthy pet dogs in a census-based, cross-sectional study in cheshire; culture methods were used to detect any campylobacter species and a direct pcr was used to detect campylobacter upsaliensis. forty-six of the dogs were positive for c upsaliensis by either culture or direct pcr, giving a prevalence of 25.1 per cent (95 per cent confidence interval [ci] 19.0 to 32.1 per cent). one sample was positive by culture for campylobacter jejuni (95 per cent ci 0.0 ... | 2009 | 19880860 |
| survey of thermophilic campylobacter species in cats and dogs in north-western nigeria. | this study was conducted in north-western nigeria to investigate the role of cats and dogs as potential reservoirs of thermophilic campylobacter species. faecal samples were analysed from 104 cats and 141 dogs between march 2007 and march 2009. the samples were collected from animals in households, those presented to veterinary premises and feline colonies. campylobacter spp. were isolated from 39 (27.7%) and 19 (18.3%) dogs and cats, respectively. there was no significant difference in isolatio ... | 2010 | 21120798 |
| factors related to campylobacter spp. carriage in client-owned dogs visiting veterinary clinics in a region of ontario, canada. | summaryfrom july 2008 until may 2009, 240 client-owned pet dogs from seven veterinary clinics in the region of waterloo, ontario, canada participated in a study to determine pet-related management factors that may be associated with the presence of campylobacter spp. in dogs. the prevalence of campylobacter spp. carriage in our study population of pet dogs was 22%, with 19% of the dogs positive for c. upsaliensis, and 3% positive for c. jejuni. a significant risk factor from multivariable logist ... | 2011 | 21208515 |
| campylobacter troglodytis sp. nov., isolated from feces of human-habituated wild chimpanzees (pan troglodytes schweinfurthii) in tanzania. | the transmission of simian immunodeficiency and ebola viruses to humans in recent years has heightened awareness of the public health significance of zoonotic diseases of primate origin, particularly from chimpanzees. in this study, we analyzed 71 fecal samples collected from 2 different wild chimpanzee (pan troglodytes) populations with different histories in relation to their proximity to humans. campylobacter spp. were detected by culture in 19/56 (34%) group 1 (human habituated for research ... | 2011 | 21278267 |
| eleutherine americana: a candidate for the control of campylobacter species. | the antibacterial activity of ethanolic extracts of selected thai medicinal plants (rhodomyrtus tomentosa (aiton) hassk., quercus infectoria g. olivier, and eleutherine americana merr.) against campylobacter spp. was investigated. sixty-five campylobacter, including 39 isolates from humans and 26 isolates from chicken samples, were tested. reference campylobacter spp. that are commonly encountered in gastroenteritis were included. the ethanolic extract of e. americana demonstrated good antibacte ... | 2011 | 21406364 |
| differentiation of campylobacter species by surface-enhanced laser desorption/ionization-time-of-flight mass spectrometry. | abstract the genus campylobacter contains several, widespread pathogens causing food-borne diseases of zoonotic nature in humans. in case of outbreaks, the differentiation of closely related campylobacter is essential for epidemiological studies, which investigate the routes of geographical spread and ways of transmission. recent advances in mass spectrometry (ms) have shown that matrix-assisted laser desorption/ionization-time-of-flight (maldi-tof) ms is a valuable tool for speciation of bacter ... | 2011 | 21524195 |
| emerging dynamics of human campylobacteriosis in southern ireland. | infections with campylobacter spp. pose a significant health burden worldwide. the significance of campylobacter jejuni/campylobacter coli infection is well appreciated but the contribution of non-c. jejuni/c. coli spp. to human gastroenteritis is largely unknown. in this study, we employed a two-tiered molecular study on 7194 patient faecal samples received by the microbiology department in cork university hospital during 2009. the first step, using entericbio(®) (serosep), a multiplex pcr syst ... | 2011 | 22077228 |
| comparative characterization of the virulence gene clusters (lipooligosaccharide [los] and capsular polysaccharide [cps]) for campylobacter coli, campylobacter jejuni subsp. jejuni and related campylobacter species. | campylobacter jejuni subsp. jejuni and campylobacter coli are leading causes of gastroenteritis, with virulence linked to cell surface carbohydrate diversity. although the associated gene clusters are well studied for c. jejuni subsp. jejuni, c. coli has been largely neglected. here we provide comparative analysis of the lipooligosaccharide (los) and capsular polysaccharide (cps) gene clusters, using genome and cluster sequence data for 36 c. coli strains, 67 c. jejuni subsp. jejuni strains and ... | 2012 | 23279811 |
| association of campylobacter upsaliensis with persistent bloody diarrhea. | campylobacter upsaliensis is a zoonotic, emerging pathogen that is not readily recovered in traditional stool culture. this case represents the first report of persistent bloody diarrhea with c. upsaliensis that was confirmed by filtration culture, pcr, and sequencing. | 2012 | 22915607 |
| first case report of fatal sepsis due to campylobacter upsaliensis. | we encountered a rare case of severe fatal infection in a 70-year-old woman due to campylobacter upsaliensis, identified by pcr amplification and sequencing analysis of the 16s rrna gene using dna extracted from the isolates. to our knowledge, fatal sepsis due to this organism has never been described to date. | 2014 | 25411172 |