| pectin decomposition and associated nitrogen fixation by mixed cultures of azospirillum and bacillus species. | cocultures of different azospirillum species with bacillus polymyxa or bacillus subtilis allow the efficient utilization of pectin as carbon and energy sources for nitrogen fixation. the nitrogenase activity obtained with cocultures was as high as 30-80 nmol c2h4 h-1 ml-1, a much higher value than that obtained with pure cultures of either azospirillum (up to 13 nmol c2h4 h-1 ml-1) or b. polymyxa (up to 2 nmol c2h4 h-1 ml-1) alone. to establish to what extent each partner contributed to nitrogen ... | 1992 | 1458371 |
| membrane protein patterns of three azospirillum species and herbaspirillum seropedicae. | membrane protein patterns in sds-page clearly characterized azospirillum amazonense, a. brasilense, a. lipoferum, and herbaspirillum seropedicae. each species showed also a typical major band with approximate molecular weights (amws) of 44, 39.5, 35.5, and 37.5 kdaltons for the four species respectively. characteristic lighter bands with amws of 63.5 and 19 kdaltons were shown by a. amazonense. similar bands of 54, 24 and 24.5 kdaltons were typical of a. brazilense, a. lipoferum and h. seropedic ... | 1989 | 2629550 |
| regulation of nitrogenase activity by ammonium chloride in azospirillum spp. | ammonium chloride (greater than or equal to 0.05 mm) effectively and reversibly inhibited the nitrogenase activity of azospirillum brasilense, azospirillum lipoferum and azospirillum amazonense. the glutamine synthetase inhibitor l-methionine-dl- sulfoximine abolished this "switch-off" in a. lipoferum and a. brasilense, but not in a. amazonense. azaserine, an inhibitor of glutamate synthase, inhibited nitrogenase activity itself. this provides further evidence for glutamine as a metabolite of re ... | 1986 | 3081492 |
| purification and properties of the nitrogenase of azospirillum amazonense. | the nitrogenase of the free-living, microaerobic, n2-fixing bacterium azospirillum amazonense (strain y1) was purified by chromatography on deae-52 cellulose, by heat treatment, and by preparative polyacrylamide gel electrophoresis. the specific nitrogenase activities were 2,400 nmol of c2h4 formed per min per mg of protein for dinitrogenase (mofe protein) and 1,800 nmol of c2h4 formed per min per mg of protein for dinitrogenase reductase (fe protein). the mofe protein was composed of a minimum ... | 1985 | 3864779 |
| [azospirillum amazonense: its presence in the sugar cane area of the province of tucumán]. | we confirm the presence of azospirillum amazonense in the sugar cane area of the province of tucumán. several strains were isolated from sugar-cane roots in different places. phenotypic and genotypic characteristics coincided with those described for this species. this new one is being added to the other two species, a. brasilense and a. lipoferum, already reported. | 2000 | 11149152 |
| partial characterization of nif genes from the bacterium azospirillum amazonense. | azospirillum amazonense revealed genomic organization patterns of the nitrogen fixation genes similar to those of the distantly related species a. brasilense. our work suggests that a. brasilense nifhdk, nifenx, fixabc operons and nifa and glnb genes may be structurally homologous to the counterpart genes of a. amazonense. this is the first analysis revealing homology between a. brasilense nif genes and the a. amazonense genome. sequence analysis of pcr amplification products revealed similariti ... | 2001 | 11514833 |
| molecular and functional characteristics, growth promoting effect and persistence of selected parent isolates and streptomycin resistant derivatives of rice rhizobacteria. | molecular and functional characteristics of seven azospirilla and five phosphorus solubilizing bacteria (psb) isolates of rice rhizosphere, growth promotion ability of two efficient strains, azospirillum amazonense a10 (mtcc4716) and bacillus megaterium p5 (mtcc4714) and their persistence based on streptomycin resistant derivatives (srd), were determined. sds-page and isozyme banding patterns of the isolates were used to arbitrarily group the azospirilla into 4 and psb into 3 clusters and as mar ... | 2004 | 15623229 |
| carbohydrate catabolism in azospirillum amazonense. | the nitrogen fixer azospirillum amazonense grew on the various disaccharides, hexoses, and pentoses tested in this study but not on polyols and on some tricarboxylic acid cycle intermediates. an active transport system was detected for sucrose and glucose but not for mannitol and 2-ketoglutarate. six a. amazonense strains were examined for 16 carbon-metabolizing enzymes, and the results indicate that these strains employ the entner-doudoroff pathway to catabolize sucrose, fructose, and glucose. ... | 1985 | 16346836 |
| rapid detection and identification of the free-living nitrogen fixing genus azospirillum by 16s rrna-gene-targeted genus-specific primers. | the modern agricultural practice utilizing plant growth promoting rhizobacteria (pgpr) has brought great benefits in the promotion of crop growth. among pgpr, azospirillum is considered as an important genus which is not only closely-associated with plants but also shows potential in the degradation of organic contaminants. however, lack of media for selective isolation or techniques for specific detection or identification limit the exploration of these rhizobacteria. this motivated us to desig ... | 2011 | 21327476 |
| tools for genetic manipulation of the plant growth-promoting bacterium azospirillum amazonense. | abstract: background: azospirillum amazonense has potential to be used as agricultural inoculant since it promotes plant growth without causing pollution, unlike industrial fertilizers. owing to these facts, the study of this species has gained interest. however, a detailed understanding of its genetics and physiology is limited by the absence of appropriate genetic tools for the study of this species. results: conjugation and electrotransformation methods were established utilizing vectors with ... | 2011 | 21575234 |
| genomic insights into the versatility of the plant growth-promoting bacterium azospirillum amazonense. | abstract: | 2011 | 21838888 |
| the plant growth-promoting bacteria azospirillum amazonense: genomic versatility and phytohormone pathway. | the rhizosphere bacterium azospirillum amazonense associates with plant roots to promote plant growth. variation in replicon numbers and rearrangements is common among azospirillum strains, and characterization of these naturally occurring differences can improve our understanding of genome evolution. we performed an in silico comparative genomic analysis to understand the genomic plasticity of a. amazonense. the number of a. amazonense-specific coding sequences was similar when compared with th ... | 2015 | 25866821 |
| the ipdc, hisc1 and hisc2 genes involved in indole-3-acetic production used as alternative phylogenetic markers in azospirillum brasilense. | plant growth-promoting bacteria of the genus azospirillum are present in the rhizosphere and as endophytes of many crops. in this research we studied 40 azospirillum strains isolated from different plants and geographic regions. they were first characterized by 16s rdna restriction analysis, and their phylogenetic position was established by sequencing the genes 16s rdna, ipdc, hisc1, and hisc2. the latter three genes are involved in the indole-3-pyruvic acid (ipya) biosynthesis pathway of indol ... | 2015 | 25842039 |
| population dynamics of free living, nitrogen fixing bacteria azospirillum in manakkudi mangrove ecosystem, india. | seasonal variations of population dynamics of free living nitrogen fixing bacteria, azospirillum in relation to chemical parameters in manakkudi mangrove ecosystem was assessed in root and rhizosphere soil samples of mangroves and mangrove associated plants. in rhizosphere soil and root samples, the counts of azospirillum were recorded maximum in acrostichum aureum as 8.63 +/- 0.92 x 10(4) and 115.48 +/- 17.36 x 10(4) cfu g(-1), respectively. the counts of azospirillum in non-rhizosphere soil va ... | 2012 | 23029909 |
| development and nitrate reductase activity of sugarcane inoculated with five diazotrophic strains. | diazotrophs are able to stimulate plant growth. this study aimed at evaluating the effect of inoculation of five diazotrophic strains on growth promotion and nitrate reductase (nr, ec 1.7.1.1) activity in sugarcane. an experiment was carried out from three stages of cultivation: sprouting, tubes, and in hydroponics. on the first two stages, seven treatments were adopted: uninoculated control; mixed inoculation with five strains; and individual inoculation with gluconacetobacter diazotrophicus (g ... | 2017 | 28321482 |
| nitrospirillum irinus sp. nov., a diazotrophic bacterium isolated from the rhizosphere soil of iris and emended description of the genus nitrospirillum. | a polyphasic approach was used to characterize a novel nitrogen-fixing bacterial strain, designated yc6995(t), isolated from the rhizosphere soil of iris ensata var. spontanea (makino) nakai inhabiting a wetland located at an altitude of 960 m on jiri mountain, korea. strain yc6995(t) cells were gram-negative, and rod-shaped, with motility provided by a single polar flagellum. optimal growth conditions were 30 °c and ph 7.0. the major fatty acids of strain yc6995(t) were c18:1 ω7c, c18:1 2-oh an ... | 2015 | 26155773 |
| description of niveispirillum fermenti gen. nov., sp. nov., isolated from a fermentor in taiwan, transfer of azospirillum irakense (1989) as niveispirillum irakense comb. nov., and reclassification of azospirillum amazonense (1983) as nitrospirillum amazonense gen. nov. | a taxonomic study was carried out on a novel aerobic bacterial strain (designated cc-ly736(t)) isolated from a fermentor in taiwan. cells of strain cc-ly736(t) were gram-stain negative, spiral-shaped and motile by means of a monopolar flagellum. strain cc-ly736(t) shared the greatest degree of 16s rrna gene sequence similarity to azospirillum irakense dsm 11586(t) (97.2 %), rhodocista centenaria jcm 21060(t) (96.3 %) and rhodocista pekingensis jcm 11669(t) (96.1 %). the major fatty acids were c1 ... | 2014 | 24771126 |