| a numerical classification of the genus bacillus. | three hundred and sixty-eight strains of aerobic, endospore-forming bacteria which included type and reference cultures of bacillus and environmental isolates were studied. overall similarities of these strains for 118 unit characters were determined by the ssm, sj and dp coefficients and clustering achieved using the upgma algorithm. test error was within acceptable limits. six cluster-groups were defined at 70% ssm, which corresponded to 69% sp and 48-57% sj. groupings obtained with the three ... | 1988 | 3246588 |
| phylogenetic analysis of bacillus sphaericus and development of an oligonucleotide probe specific for mosquito-pathogenic strains. | the 16s rrna gene sequences for six strains of bacillus sphaericus representing five distinct dna homology groups and one strain of bacillus fusiformis have been determined. an-oligonucleotide probe based on the area approximately between position 186 and 198 (escherichia coli numbering) of the 16s rrna gene of the mosquito pathogen, strain 2362, hybridised to dna from strains of dna homology group iia which contains all known mosquito pathogens and not to any of ten other bacillus species. it c ... | 1993 | 7691684 |
| phylogenetic interrelationships of round-spore-forming bacilli containing cell walls based on lysine and the non-spore-forming genera caryophanon, exiguobacterium, kurthia, and planococcus. | the 16s rrna gene sequences of "bacillus aminovorans" and several species considered to be phylogenetically related to the group 2 bacilli of ash et al. (c. ash, j. a. e. farrow, s. wallbanks, and m. d. collins, lett. appl. microbiol. 13:202-206, 1991) were determined. a comparative analysis of the sequence data revealed that the round-spore-forming group 2 bacilli, together with some asporogenous taxa (the genera caryophanon, exiguobacterium, kurthia, planococcus), form a phylogenetically disti ... | 1994 | 8123563 |
| labelled trinucleotides as quantitative probes to identify bacillus spp. using fluorescent in situ hybridization. | the number of nucleotide triplet repeats in 16 s rrna sequences can be used for detection and identification of bacteria. labelled ttt, ggg and ata triplets were hybridized to the ribonucleic acid of bacillus subtilis and bacillus fusiformis whole-cells and the number of such triplets was quantified by synchronous fluorescence spectrometry. each species was distinctly identified by specific ratios of labelled ttt, ggg and ata triplets as well as characteristic fluorescence spectra. notwithstandi ... | 2000 | 10799269 |
| phylogeny of bacillus sphaericus-like organisms. | the mesophilic round-spored bacteria embrace four species, namely bacillus sphaericus, bacillus fusiformis, bacillus silvestris and bacillus pasteurii. although not displayed by all strains, mosquito pathogenicity is a noteworthy characteristic of b. sphaericus sensu lato. phylogenetic analysis based on 16s rdna sequences from 58 strains identified as b. sphaericus was used to examine the genetic heterogeneity of the taxon. results from sequence analysis were compared with whole-cell fatty acid ... | 2000 | 11034479 |
| bacillus pycnus sp. nov. and bacillus neidei sp. nov., round-spored bacteria from soil. | bacillus sphaericus sensu lato currently consists of seven or more groups of unrelated taxa, one of which is b. sphaericus sensu stricto and another of which is bacillus fusiformis. members of two groups (groups 6 and 7), in common with all other b. sphaericus-like organisms, are unable to grow anaerobically or to use common hexoses, pentoses and hexitols as sources of carbon, have g+c contents of 34-36 mol % and form round spores. groups 6 and 7 can be differentiated from other b. sphaericus-li ... | 2002 | 11931162 |
| phylogenetic relationships between bacillus species and related genera inferred from comparison of 3' end 16s rdna and 5' end 16s-23s its nucleotide sequences. | the nucleotide sequences of the 3' end of the 16s rdna and the 16s-23s internal transcribed spacer (its) of 40 bacillaceae species were determined. these included 21 bacillus, 9 paenibacillus, 6 brevibacillus, 2 geobacillus, 1 marinibacillus and 1 virgibacillus species. comparative sequence analysis of a 220 bp region covering a highly conserved 150 bp sequence located at the 3' end of the 16s rrna coding region and a conserved 70 bp sequence located at the 5' end of the 16s-23s its of the 40 sp ... | 2003 | 12807189 |
| [observation and comparison on microstructure of immobilized aerobacillus fusiformis under wastewater treatment]. | aiming at printing ink wastewater, a strain of bacterium was isolated from activated sludge, and identified as aerobacillus fusiformis. the aerobacillus fusiformis was respectively immobilized in calcium alginate gel, polyvinyl alcohol (pva) and microporous ceramics and their microstructure were observed by scanning electron microscope (sem). it was found that calcium alginate gel might dissolve in the wastewater, and the activity was restricted as cells wrapped in pva, so the cod removal ratio ... | 2003 | 12916206 |
| [two cases of inflammatory lesions of necrotic type due to spriochaeta vincenti with bacillus fusiformis]. | | 1953 | 13147737 |
| [bacillus fusiformis in intrabronchial aspirations]. | | 1963 | 14104720 |
| bacillus odysseyi sp. nov., a round-spore-forming bacillus isolated from the mars odyssey spacecraft. | a round-spore-forming bacillus species that produces an exosporium was isolated from the surface of the mars odyssey spacecraft. this novel species has been characterized on the basis of phenotypic traits, 16s rdna sequence analysis and dna-dna hybridization. according to the results of these analyses, this strain belongs to the genus bacillus and is a gram-positive, aerobic, rod-shaped, endospore-forming eubacterium. ultrathin sections of the spores showed the presence of an exosporium, spore c ... | 2004 | 14742480 |
| insecticidal bacillus thuringiensis silences erwinia carotovora virulence by a new form of microbial antagonism, signal interference. | it is commonly known that bacteria may produce antibiotics to interfere with the normal biological functions of their competitors in order to gain competitive advantages. here we report that bacillus thuringiensis suppressed the quorum-sensing-dependent virulence of plant pathogen erwinia carotovora through a new form of microbial antagonism, signal interference. e. carotovora produces and responds to acyl-homoserine lactone (ahl) quorum-sensing signals to regulate antibiotic production and expr ... | 2004 | 14766576 |
| acute gastro-enteritis due to t. vincenti and b. fusiformis. | | 1950 | 14794202 |
| fermentative processes of the fusiform bacteria. | | 1951 | 14824086 |
| isolation and characterization of a bacterium capable of removing taste- and odor-causing 2-methylisoborneol from water. | 2-methylisoborneol (mib), a metabolite of blue-green algae, has been implicated in causing unpalatable drinking water throughout the world. current non-biological water treatment technologies are ineffective in removing mib from potable water or are cost-prohibitive, and biological applications may address these problems. we have isolated and characterized a bacterium derived from lake water and capable of aerobically degrading mib. light microscopy and transmission electron microscopy revealed ... | 2004 | 15491661 |
| study of mural painting isolates, leading to the transfer of 'bacillus maroccanus' and 'bacillus carotarum' to bacillus simplex, emended description of bacillus simplex, re-examination of the strains previously attributed to 'bacillus macroides' and description of bacillus muralis sp. nov. | a group of 24 strains was isolated from deteriorated mural paintings situated in spain (necropolis of carmona) and germany (church of greene-kreiensen). (gtg)5-pcr genomic fingerprinting was performed on these strains to assess their genomic variability and the strains were delineated into four groups. representatives were studied by 16s rrna gene sequencing and were found to be closely related to bacillus simplex and the species 'bacillus macroides' (strain ncimb 8796) and 'bacillus maroccanus' ... | 2005 | 15653864 |
| isolation and characterization of diazotrophic growth promoting bacteria from rhizosphere of agricultural crops of korea. | free-living nitrogen fixing bacteria were isolated from rhizosphere of seven different plant namely sesame, maize, wheat, soybean, lettuce, pepper and rice grown in chungbuk province, korea. five isolates with nitrogenase activity above 150nmol(-1) mg(-1) protein were identified based on, phenotypic and 16s rdna sequences analysis. the strains were identified as stenotrophomonas maltophilia (pm-1, pm-26), bacillus fusiformis (pm-5, pm-24) and pseudomonas fluorescens (pm-13), respectively. all th ... | 2005 | 15881829 |
| biotransformation of isoeugenol to vanillin by a novel strain of bacillus fusiformis. | a novel strain of bacillus fusiformis, producing high amounts of vanillin from isoeugenol, was isolated from soil. using 60% (v/v) isoeugenol as substrate and solvent and at ph 4.0, 37 degrees c and 180 rpm, vanillin was produced at 32.5 g l(-1) over 72 h. the unused isoeugenol was reusable. | 2005 | 16231224 |
| alkane utilization by rhodococcus strain ntu-1 alone and in its natural association with bacillus fusiformis l-1 and ochrobactrum sp. | linear (n-hexadecane) and branched (pristane) alkanes were degraded by a mixed culture isolated from an oil-contaminated field. the degradation was accompanied by formation of biofloccules. the culture was composed of rhodococcus strain ntu-1, bacillus fusiformis l-1, and ochrobactrum sp. rhodococcus strain ntu-1 carried out the degradation of the alkane via a hydroxylase. bacillus fusiformis l-1 and ochrobactrum sp. did not degrade the alkanes but aided the flocculation by forming more rigid ba ... | 2006 | 17022676 |
| proposal of lysinibacillus boronitolerans gen. nov. sp. nov., and transfer of bacillus fusiformis to lysinibacillus fusiformis comb. nov. and bacillus sphaericus to lysinibacillus sphaericus comb. nov. | three strains of a spore-forming, gram-positive, motile, rod-shaped and boron-tolerant bacterium were isolated from soil. the strains, designated 10a(t), 11c and 12b, can tolerate 5 % (w/v) nacl and up to 150 mm boron, but optimal growth was observed without addition of boron or nacl in luria-bertani agar medium. the optimum temperature for growth was 37 degrees c (range 16-45 degrees c) and the optimum ph was 7.0-8.0 (range ph 5.5-9.5). a comparative analysis of the 16s rrna gene sequence demon ... | 2007 | 17473269 |
| isolation and algae-lysing characteristics of the algicidal bacterium b5. | water blooms have become a worldwide environmental problem. recently, algicidal bacteria have attracted wide attention as possible agents for inhibiting algal water blooms. in this study, one strain of algicidal bacterium b5 was isolated from activated sludge. on the basis of analysis of its physiological characteristics and 16s rdna gene sequence, it was identified as bacillus fusiformis. its algaelysing characteristics on microcystis aeruginosa, chlorella and scenedesmus were tested. the resul ... | 2007 | 18232228 |
| evaluation of in vitro cr(vi) reduction potential in cytosolic extracts of three indigenous bacillus sp. isolated from cr(vi) polluted industrial landfill. | three efficient cr(vi) reducing bacterial strains were isolated from cr(vi) polluted landfill and characterized for in vitro cr(vi) reduction. phylogenetic analysis using 16s rrna gene sequencing revealed that the newly isolated strains g1dm20, g1dm22 and g1dm64 were closely related to bacillus cereus, bacillus fusiformis and bacillus sphaericus, respectively. the suspended cultures of all bacillus sp. exhibited more than 85% reduction of 1000 microm cr(vi) within 30 h. the suspended culture of ... | 2008 | 18255287 |
| decolorization and degradation of disperse blue 79 and acid orange 10, by bacillus fusiformis kmk5 isolated from the textile dye contaminated soil. | the release of azo dyes into the environment is a concern due to coloration of natural waters and due to the toxicity, mutagenicity and carcinogenicity of the dyes and their biotransformation products. the dye degrading bacterial strain kmk 5 was isolated from the textile dyes contaminated soil of ichalkaranji, maharashtra, india. it was identified as bacillus fusiformis based on the biochemical and morphological characterization as well as 16s rdna sequencing. kmk 5 could tolerate and degrade a ... | 2008 | 18562194 |
| a novel biotransformation process of 4'-demethylepipodophyllotoxin to 4'-demethylepipodophyllic acid by bacillus fusiformis cicc 20463, part ii: process optimization. | this work optimized the novel biotransformation process of 4'-demethylepipodophyllotoxin (dmep) into 4'-demethylepipodophyllic acid (dmepa) by bacillus fusiformis cicc 20463. firstly, the biotransformation process was significantly affected by medium composition. 5 g/l of yeast extract and 10 g/l of peptone were optimal for dmepa production (i.e., 2.81 + or - 0.21 mg/l), while not beneficial for the cell growth of b. fusiformis. this indicated that the biosynthesis of dmepa was not corresponded ... | 2010 | 19396467 |
| lysinibacillus parviboronicapiens sp. nov., a low-boron-containing bacterium isolated from soil. | a spore-forming, gram-positive-staining, motile, rod-shaped and low-boron-containing bacterium was isolated from soil. the strain, designated bam-582(t), can tolerate 6 % (w/v) nacl and 50 mm boron, but optimal growth was observed without addition of boron or nacl. the optimum temperature and ph for growth were 30 degrees c (range 10-37 degrees c) and ph 7 (range ph 6-8). a comparative analysis of the 16s rrna gene sequence demonstrated that the isolated strain was closely related to lysinibacil ... | 2009 | 19502328 |
| toxinogenic and spoilage potential of aerobic spore-formers isolated from raw milk. | the harmful effects on the quality and safety of dairy products caused by aerobic spore-forming isolates obtained from raw milk were characterized. quantitative assessment showed strains of bacillus subtilis, the bacillus cereus group, paenibacillus polymyxa and bacillus amyloliquefaciens to be strongly proteolytic, along with bacillus licheniformis, bacillus pumilus and lysinibacillus fusiformis to a lesser extent. lipolytic activity could be demonstrated in strains of b. subtilis, b. pumilus a ... | 2010 | 19944473 |
| morphological changes in bacillus fusiformis. | | 1946 | 20275759 |
| characteristic morphological changes in bacillus fusiformis in the course of penicillin treatment. | | 1947 | 20294247 |
| calcite-forming bacteria for compressive strength improvement in mortar. | microbiological calcium carbonate precipitation (mcp) has been investigated for its ability to improve the compressive strength of concrete mortar. however, very few studies have been conducted on the use of calcite-forming bacteria (cfb) to improve compressive strength. in this study, we discovered new bacterial genera that are capable of improving the compressive strength of concrete mortar. we isolated 4 cfb from 7 environmental concrete structures. using sequence analysis of the 16s rrna gen ... | 2010 | 20467254 |
| inoculation experiments with bacillus fusiformis isolated from tropical ulcer with observations on the bacillus. | | 1933 | 20475172 |
| biochemical properties of pectate lyases produced by three different bacillus strains isolated from fermenting cocoa beans and characterization of their cloned genes. | pectinolytic enzymes play an important role in cocoa fermentation. in this study, we characterized three extracellular pectate lyases (pels) produced by bacilli isolated from fermenting cocoa beans. these enzymes, named pel-22, pel-66, and pel-90, were synthesized by bacillus pumilus bs22, bacillus subtilis bs66, and bacillus fusiformis bs90, respectively. the three pels were produced under their natural conditions and purified from the supernatants using a one-step chromatography method. the pu ... | 2010 | 20543060 |
| isolation of a lysinibacillus fusiformis strain with tetrodotoxin-producing ability from puffer fish fugu obscurus and the characterization of this strain. | six dominant strains were isolated from the livers of the puffer fish fugu obscurus and were screened for their ttx production-ability. electrospray ionization-mass spectrometry (esi-ms) analyses revealed that strain b-1 produced ttx and related substances; mouse bioassay revealed that 23.9 mouse units (mu) of toxins were present in 200ml of broth medium. on the basis of the physiological and biochemical characteristics of this strain and the results of 16s rrna analysis, strain b-1 was identifi ... | 2010 | 20576513 |
| biodegradation of naphthalene by strain bacillus fusiformis (bfn). | bacterial strains isolated from oil refining wastewater sludge (fuzhou, china) were used to biodegrade naphthalene in cultured medium. bacillus fusiformis (bfn) strain was identified using 16s rdna gene sequence analysis. optimal conditions for the biodegradation of naphthalene included: temperature of 30 degrees c, ph 7.0, 0.2% inoculum size and a c/n ratio of 1.0. under these conditions and initial naphthalene concentration of 50 mg/l, more than 99.1% was removed within 96 h. of those factors ... | 2010 | 20643503 |
| characterization and genomic analysis of a highly chromate resistant and reducing bacterial strain lysinibacillus fusiformis zc1. | lysinibacillus fusiformis zc1 isolated from chromium (cr) contaminated wastewater of a metal electroplating factory displayed high chromate [cr(vi)] resistance with a minimal inhibitory concentration (mic) of 60mm in r2a medium. l. fusiformis zc1 showed resistances to multiple metals (cu, ni, co, hg, cd and ag) and a metalloid (as). this bacterium exhibited an extremely rapid cr(vi) reduction capability. it almost completely reduced 1mm k(2)cro(4) in 12h. the cr(vi) reduction ability of l. fusif ... | 2010 | 20952126 |
| changes occurring in bacillus fusiformis during the application of penicillin. | | 1946 | 20991744 |
| diversity of endophytic bacteria in ginseng and their potential for plant growth promotion. | endophytic bacteria have been found in virtually every plant studied, where they colonize the internal tissues of their host plant and can form a range of different beneficial relationships. the diversity of bacterial endophytes associated with ginseng plants of varying age levels in korea was investigated. fifty-one colonies were isolated from the interior of ginseng stems. although a mixed composition of endophyte communities was recovered from ginseng based on the results of 16s rdna analysis ... | 2010 | 21046332 |
| molecular identification and pectate lyase production by bacillus strains involved in cocoa fermentation. | we have previously reported the implication of bacillus in the production of pectinolytic enzymes during cocoa fermentation. the objective of this work was to identify the bacillus strains isolated from cocoa fermentation and study their ability to produce pectate lyase (pl) in various growth conditions. ninety-eight strains were analyzed by amplified ribosomal dna restriction analysis (ardra). four different banding patterns were obtained leading to the clustering of the bacterial isolates into ... | 2010 | 21056768 |
| isolation and characterization of beneficial bacteria associated with citrus roots in florida. | cultivable diversity of bacteria associated with citrus was investigated as part of a larger study to understand the roles of beneficial bacteria and utilize them to increase the productive capacity and sustainability of agro-ecosystems. citrus roots from huanglongbing (hlb) diseased symptomatic and asymptomatic citrus were used in this study. a total of 227 and 125 morphologically distinct colonies were isolated and characterized from hlb asymptomatic and symptomatic trees, respectively. we obs ... | 2011 | 21360139 |
| isolation and characterization of lead-tolerant ochrobactrum intermedium and its role in enhancing lead accumulation by eucalyptus camaldulensis. | in this study, the potential of rhizospheric bacteria in promoting the growth and pb accumulation by the woody plant eucalyptus camaldulensis under hydroponic conditions was investigated for the first time. three pb-tolerant bacteria were isolated from the rhizosphere of e. camaldulensis grown in pb-contaminated soils in the bo ngam pb mine, thailand. based on analysis of partial 16s rrna gene sequence, the three isolates were identified as microbacterium paraoxydans bn-2, ochrobactrum intermedi ... | 2011 | 21764101 |
| lysinibacillus macroides sp. nov., nom. rev. | 'bacillus macroides' atcc 12905t (= dsm 54t = lmg 18474t), isolated in 1947 from cow dung, was not included in the approved lists of bacterial names and so it lost standing in bacteriological nomenclature. reinvestigation of the strain, including dna-dna relatedness experiments, revealed that 'bacillus macroides' is genomically distinct from its closest relatives lysinibacillus xylanilyticus, lysinibacillus boronitolerans and lysinibacillus fusiformis (as determined by 16s rrna gene sequence ana ... | 2011 | 21724959 |
| Production of 10-hydroxystearic acid from oleic acid and olive oil hydrolyzate by an oleate hydratase from Lysinibacillus fusiformis. | A recombinant enzyme from Lysinibacillus fusiformis was expressed, purified, and identified as an oleate hydratase because the hydration activity of the enzyme was the highest for oleic acid (with a k (cat) of 850 min(-1) and a K (m) of 540 µM), followed by palmitoleic acid, ?-linolenic acid, linoleic acid, myristoleic acid, and a-linolenic acid. The optimal reaction conditions for the enzymatic production of 10-hydroxystearic acid were pH 6.5, 35 °C, 4% ... | 2011 | 22189865 |
| microbial biofilms on the sandstone monuments of the angkor wat complex, cambodia. | discoloring biofilms from cambodian temples angkor wat, preah khan, and the bayon and west prasat in angkor thom contained a microbial community dominated by coccoid cyanobacteria. molecular analysis identified chroococcidiopsis as major colonizer, but low similarity values (<95%) suggested a similar genus or species not present in the databases. in only two of the six sites sampled were filamentous cyanobacteria, microcoleus, leptolyngbya, and scytonema, found; the first two detected by sequenc ... | 2011 | 22006074 |
| Two-phase olive mill waste composting: community dynamics and functional role of the resident microbiota. | In this study, physico-chemical modifications and community dynamics and functional role of the resident microbiota during composting of humid husk from a two-phase extraction system (TPOMW) were investigated. High mineralization and humification of carbon, low loss of nitrogen and complete degradation of polyphenols led to the waste biotransformation into a high-quality compost. Viable cell counts and denaturing gradient gel electrophoresis (DGGE) profiling of the 16S rRNA genes showed that the ... | 2011 | 21996482 |
| Description of Lysinibacillus sinduriensis sp. nov., and transfer of Bacillus massiliensis and Bacillus odysseyi to Lysinibacillus as Lysinibacillus massiliensis comb. nov. and Lysinibacillus odysseyi comb. nov. with emended description of the genus. | A Gram-positive, rod-shaped, endospore-forming bacterium, strain BLB-1T, was isolated from the samples of tidal flat sediment in the Yellow Sea. The 16S rRNA gene sequence analysis demonstrated that the isolate belonged to the Bacillus rRNA group 2 and it was closely related to Bacillus massiliensis CIP 108446T (97.4%), Bacillus odysseyi ATCC PTA-4993T (96.7%), Lysinibacillus fusiformis DSM 2898T (96.2%), and Lysinibacillus boronitolerans DSM 17140T (95.9%). Sequence similarities with species in ... | 2011 | 22140163 |
| draft genome sequence of lysinibacillus fusiformis strain sw-b9, a novel strain for biotransformation of isoeugenol to vanillin. | lysinibacillus fusiformis sw-b9 was the first reported strain in l. fusiformis showing effective biotransformation of isoeugenol to vanillin. here, we report the annotated genome of strain sw-b9, which has special pathways for producing vanillin. the genome will provide a genetic basis for better understanding the physiology of this species. | 2015 | 25883291 |
| isolation and molecular identification of landfill bacteria capable of growing on di-(2-ethylhexyl) phthalate and deteriorating pvc materials. | waste materials containing di-(2-ethylhexyl) phthalate (dehp), a suspected endocrine disruptor and reasonably anticipated human carcinogen, are typically disposed of in landfills. despite this, very few studies had been conducted to isolate and identify dehp-degrading bacteria in landfill leachate. therefore, this study was conducted to isolate and characterize bacteria in landfill leachate growing on dehp as the sole carbon source and deteriorating pvc materials. four strains lhm1, lhm2, lhm3 a ... | 2012 | 22934997 |
| structure and function of the bacterial communities during rhizoremediation of hexachlorobenzene in constructed wetlands. | vertical flow constructed wetlands (vf cws) are considered to be effective for treating organic pollutants. the rhizosphere of macrophytes such as phragmites sp., typha sp. serves as an active and dynamic zone for the microbial degradation of organic pollutants. however, it is still not clear how soil bacterial communities respond to macrophytes and pollutants during the process. for this purpose, the seedlings of phragmites australis and typha angustifolia were planted respectively in the vf cw ... | 2017 | 28316049 |
| rhizosphere of avicennia marina (forsk.) vierh. as a landmark for polythene degrading bacteria. | due to high durability, cheap cost, and ease of manufacture, 311 million tons of plastic-based products are manufactured around the globe per annum. the slow/least rate of plastic degradation leads to generation of million tons of plastic waste per annum, which is of great environmental concern. of the total plastic waste generated, polythene shared about 64 %. various methods are available in the literature to tackle with the plastic waste, and biodegradation is considered as the most accepted, ... | 2016 | 27072028 |
| bacteria-based polythene degradation products: gc-ms analysis and toxicity testing. | polythene degradation leads to the production of various by-products depending upon the type of degradation process. the polythene degradation products (pedp) in the culture supernatant of the two bacteria (lysinibacillus fusiformis strain vasb14/wl and bacillus cereus strain vasb1/ts) were analyzed with gc-ms technique. the major by-products in the pedp in the culture supernatant of l. fusiformis strain vasb14/wl (1,2,3,4 tetra methyl benzene) and b. cereus strain vasb1/ts (1,2,3 trimethyl benz ... | 2016 | 26888528 |
| characterization of microfouling and corrosive bacterial community of a firewater distribution system. | this investigation provides generic information on the culturable corrosive and the microfouling bacterial community in a firewater distribution system that uses freshwater. conventional microbiological methods were used for the selective isolation of the major microfouling bacteria. the isolates were characterized by 16s rrna gene sequencing and the biofilm as well as the corrosion characteristics of the isolates were evaluated. pseudomonas aeruginosa and bacillus cereus were predominantly obse ... | 2016 | 26467696 |
| [screening of homoacetogen mixed culture converting h2/co2 to acetate]. | homoacetogens are a group of microorganisms with application potential to produce chemicals and biofuels by the bioconversion of synthesis gas. in this study, we collected waste activated sludge samples to screen homoacetogens by hungate anaerobic technique, and studied the effect of ph on acetate and alcohol production from h2/co2 gas. the mixed culture contained clostridium ljungdahlii, lysinibacillus fusiformis and bacillus cereus. acetate concentration achieved 31.69 mmol/l when the initial ... | 2014 | 26016379 |
| antimicrobial potential of bacteriocin producing lysinibacillus jx416856 against foodborne bacterial and fungal pathogens, isolated from fruits and vegetable waste. | in this study, antimicrobial potential, some probiotics properties and bacteriocin nature of lysinibacillus, isolated from fruits and vegetable waste were evaluated. for this, 125 lactobacillus isolates were tested against foodborne bacterial and fungal pathogens. among these, an isolated bacillus spp. showed significant aggregation-co-aggregation probiotics properties and potentially inhibits the foodborne gram positive microbial pathogens such as staphylococcus aureus, (22 mm zoi), staphylococ ... | 2014 | 24735603 |
| viability of calcifying bacterial formulations in fly ash for applications in building materials. | evidence of bacterial involvement in precipitation of calcium carbonates has brought a revolution in the field of applied microbiology, geotechnical sciences, environmental and civil engineering with its marked success in restoration of various building materials. for applications of these calcite binder-producing bacterial cultures, different expensive carrier materials have been used but their high costs have come in the way of their successful commercialization. in the present study, we have ... | 2013 | 24065359 |
| characterization of boron resistant and accumulating bacteria lysinibacillus fusiformis m1, bacillus cereus m2, bacillus cereus m3, bacillus pumilus m4 isolated from former mining site, hokkaido, japan. | boron is known to be widespread environmental contaminant that is relatively mobile in soil when compared to other metal contaminants. the present study made an attempt to isolate and characterize the boron resistant and accumulating bacteria from former mining site at hokkaido, japan. four potential strains m1, m2, m3 and m4 were selected based on high degree of boron and heavy metal resistances. the morphological, biochemical and 16s rdna sequencing analysis of mining bacteria revealed that th ... | 2012 | 22571522 |
| bacillus species isolated from tungrymbai and bekang, naturally fermented soybean foods of india. | tungrymbai and bekang are naturally fermented soybean foods commonly consumed in meghalaya and mizoram states of india. a total of 39 samples of tungrymbai and 43 samples of bekang were collected from different villages and markets of meghalaya and mizoram, respectively and were analysed for microbial load. in both tungrymbai and bekang, the average population of bacillus spp. was 8.2±0.1 log cfu/g. a total of 428 isolates of bacillus were isolated from tungrymbai (211) and bekang (217) for deta ... | 2015 | 25574846 |
| optimized production and characterization of a detergent-stable protease from lysinibacillus fusiformis c250r. | in this study, we aimed to optimize the cultural and nutritional conditions for protease production by lysinibacillus fusiformis strain c250r in submerged fermentation process using statistical methodology. the most significant factors (gruel, wheat bran, yeast extract, and feso4) were identified by plackett-burman design. response surface methodology (rsm) was used to determine the optimum levels of the screened factors and their interaction. under the optimized conditions, protease yield 3100u ... | 2017 | 28315440 |
| biomineralization of calcium carbonate polymorphs by the bacterial strains isolated from calcareous sites. | microbially induced calcium carbonate precipitation (miccp) is a naturally occurring biological process that has various applications in remediation and restoration of a range of building materials. in the present investigation, five ureolytic bacterial isolates capable of inducing calcium carbonate precipitation were isolated from calcareous soils on the basis of production of urease, carbonic anhydrase, extrapolymeric substances, and biofilm. bacterial isolates were identified as bacillus mega ... | 2013 | 23648862 |
| characterization of biosurfactants from indigenous soil bacteria recovered from oil contaminated sites. | three bacterial isolates (g1, g2 and g3) characterized as pseudomonas plecoglossicida, lysinibacillus fusiformis and bacillus safensis were recovered from contaminated soil of oil refinery. these bacterial isolates produced biosurfactants in msm medium in stationary phase. biosurfactants were characterized on the basis of their emulsifying properties with petrol, diesel, mobil oil and petrol engine oil. reduction in surface tension (below 40 mn m(-1)) and blood hemolysis were also included in bi ... | 2015 | 26521551 |
| bioaugmentation of potent acidogenic isolates: a strategy for enhancing biohydrogen production at elevated organic load. | the efficiency of bioaugmentation strategy for enhancing biohydrogenesis at elevated organic load was successfully evaluated by augmenting native acidogenic microflora with three acidogenic bacterial isolates viz., bacillus subtilis, pseudomonas stutzeri and lysinibacillus fusiformis related to phyla firmicutes and proteobacteria separately. hydrogen production ceased at 50g cod/l operation due to feed-back inhibition. b. subtilis augmented system showed higher h2 production followed by l. fusif ... | 2014 | 24751375 |
| cytotoxic effect of microbial biosurfactants against human embryonic kidney cancerous cell: hek-293 and their possible role in apoptosis. | two different microbial biosurfactants s9bs and chbs were isolated from lysinibacillus fusiformis s9 and bacillus tequilensis ch. cytotoxicity effect of these biosurfactants on human embryonic kidney cancerous cell (hek-293) were studied with the help of 3-(4,5-dimethylthiazol-2yl-)-2, 5-diphenyl tetrazolium bromide (mtt) assay and morphological changes were observed under inverted microscope. the biosurfactants exhibited positive cytotoxic effect on hek-293 cell line. it was found that lc50 of ... | 2014 | 25149458 |
| internalisation potential of escherichia coli o157:h7, listeria monocytogenes, salmonella enterica subsp. enterica serovar typhimurium and staphylococcus aureus in lettuce seedlings and mature plants. | the internalisation potential of listeria monocytogenes, staphylococcus aureus, escherichia coli o157:h7 and salmonella enterica subsp. enterica serovar typhimurium in lettuce was evaluated using seedlings grown in vermiculite in seedling trays as well as hydroponically grown lettuce. sterile distilled water was spiked with one of the four human pathogenic bacteria (10(5) cfu/ml) and used to irrigate the plants. the potential for pathogen internalisation was investigated over time using light mi ... | 2013 | 23708570 |
| decolourisation of acid orange 7 recalcitrant auto-oxidation coloured by-products using an acclimatised mixed bacterial culture. | this study focuses on the biodegradation of recalcitrant, coloured compounds resulting from auto-oxidation of acid orange 7 (ao7) in a sequential facultative anaerobic-aerobic treatment system. a novel mixed bacterial culture, bac-zs, consisting of brevibacillus panacihumi strain zb1, lysinibacillus fusiformis strain zb2, and enterococcus faecalis strain zl bacteria were isolated from environmental samples. the acclimatisation of the mixed culture was carried out in an ao7 decolourised solution. ... | 2014 | 24293297 |
| inhibition of pathogenic bacterial biofilm by biosurfactant produced by lysinibacillus fusiformis s9. | a biosurfactant producing microbe isolated from a river bank was identified as lysinibacillus fusiformis s9. it was identified with help of biochemical tests and 16s rrna gene phylogenetic analysis. the biosurfactant s9bs produced was purified and characterized as glycolipid. the biosurfactant showed remarkable inhibition of biofilm formation by pathogenic bacteria like escherichia coli and streptococcus mutans. it was interesting to note that at concentration of 40 μg ml(-1) the biosurfactant d ... | 2014 | 23719930 |
| draft genome sequence of the soil isolate lysinibacillus fusiformis m5, a potential hypoxanthine producer. | lysinibacillus fusiformis strain m5 is a potential hypoxanthine producer that was isolated from clay soil. here, we present the draft genome sequence that was annotated in order to facilitate future studies of l. fusiformis m5. | 2016 | 27834716 |
| kinetic characterization and fed-batch fermentation for maximal simultaneous production of esterase and protease from lysinibacillus fusiformis au01. | the simultaneous production of intracellular esterase and extracellular protease from the strain lysinibacillus fusiformis au01 was studied in detail. the production was performed both under batch and fed-batch modes. the maximum yield of intracellular esterase and protease was obtained under full oxygen saturation at the beginning of the fermentation. the data were fitted to the luedeking-piret model and it was shown that the enzyme (both esterase and protease) production was growth associated. ... | 2016 | 27737615 |
| production of a microcapsule agent of chromate-reducing lysinibacillus fusiformis zc1 and its application in remediation of chromate-spiked soil. | lysinibacillus fusiformis zc1 is an efficient cr(vi)-reducing bacterium that can transform the toxic and soluble chromate [cr(vi)] form to the less toxic and precipitated chromite form [cr(iii)]. as such, this strain might be applicable for bioremediation of cr(vi) in soil by reducing its bioavailability. the study objective was to prepare a microcapsule agent of strain zc1 for bioremediation of cr(vi)-contaminated soil. using a single-factor orthogonal array design, the optimal fermentation med ... | 2016 | 27218011 |
| multifunctionality and diversity of culturable bacterial communities strictly associated with spores of the plant beneficial symbiont rhizophagus intraradices. | arbuscular mycorrhizal fungi (amf) live in symbiosis with most crop plants and represent essential elements of soil fertility and plant nutrition and productivity, facilitating soil mineral nutrient uptake and protecting plants from biotic and abiotic stresses. these beneficial services may be mediated by the dense and active spore-associated bacterial communities, which sustain diverse functions, such as the promotion of mycorrhizal activity, biological control of soilborne diseases, nitrogen f ... | 2016 | 26805620 |
| an integrated biodegradation and nano-oxidation used for the remediation of naphthalene from aqueous solution. | the remediation of toxic persistent organic contaminants in the environment has raised a need for effective cleanup methods. in this study, an integrated remediation technique based on biodegradation of naphthalene using bacillus fusiformis and fenton oxidation of their degraded metabolites using nanoscale zero-valent iron (nzvi). a 99.0% naphthalene was biodegraded by b. fusiformis in 96h, while only 59.4% chemical oxygen demand (cod) was removed, indicating that the degraded metabolites existe ... | 2015 | 26225434 |
| therapeutic intervention and molecular characterizations of bacteriocin producing lysinibacillus sp., nov., isolated from food sample. | many bacteriocins from lactobacilli have been reported as immunostimulatory, preservatives, anticancerous and biocontroling. however, antimicrobial potential of lysinibacillus is not much reported. in this study, an attempt was made to isolate and anticipate therapeutic potential of lysinibacillus from spoiled food sample. we screened 125 lactobacilli for their antagonistic nature against food borne and disease causing bacterial and fungal pathogens. among them, one bacillus was phenotypically, ... | 2015 | 26142524 |
| insights on quorum-quenching properties of lysinibacillus fusiformis strain rb21, a malaysian municipal solid-waste landfill soil isolate, via complete genome sequence analysis. | lysinibacillus fusiformis strain rb21 is a quorum-quenching bacterium that is able to degrade quorum-sensing signaling molecules. here, we present the first complete genome sequence of l. fusiformis strain rb21. the finished genome is 4.8 mbp in size, and the quorum-quenching gene was identified. | 2015 | 25953192 |
| severe sepsis secondary to persistent lysinibacillus sphaericus, lysinibacillus fusiformis and paenibacillus amylolyticus bacteremia. | lysinibacillus and paenibacillus are pervasive bacteria rarely associated with human disease. less sophisticated microbiology techniques may frequently incorrectly identify these genera as bacillus spp., often regarded as environmental contamination. this report describes a case of severe sepsis due to persistent lysinibacillus and paenibacillus bacteremia, identified by matrix-assisted laser desorption and ionization time-of-flight mass spectroscopy and 16s rrna gene sequencing. | 2015 | 25931198 |
| biological and chemical phosphorus solubilization from pyrolytical biochar in aqueous solution. | biochar, a massive byproduct of biomass pyrolysis during biofuel generation, is a potential p source for the mitigation of p depletion. however, the chemical and biological effect of the release of p from biochar is still unclear. in this study, two types of lysinibacillus strains (lysinibacillussphaericus d-8 and lysinibacillus fusiformis a-5) were separated from a sediment and their p-solubilizing characteristics to biochar was first reported. compared with the bacterial mixture w-1 obtained f ... | 2014 | 25065807 |
| the operating performance of a biotrickling filter with lysinibacillus fusiformis for the removal of high-loading gaseous chlorobenzene. | removal of gaseous chlorobenzene (cb) by a biotrickling filter (btf) filled with modified ceramics and multi-surface hollow balls during gas-liquid mass transfer at the steady state was by microbial degradation rather than dissolution in the spray liquid or emission into the atmosphere. the btf was flexible and resistant to the acid environment of the spray liquid, with the caveat that the spray liquid should be replaced once every 6-7 days. the btf, loaded with lysinibacillus fusiformis, perfor ... | 2014 | 24930097 |
| efficient glycosylation of puerarin by an organic solvent-tolerant strain of lysinibacillus fusiformis. | a bacterial strain able to glycosylate the plant natural product puerarin was isolated from local soil in nanjing, china. it was identified as lysinibacillus fusiformis, and deposited in china general microbiological culture collection (cgmcc) under accession number 4913. incubation of this strain with puerarin led to efficient production (91.6% conversation rate) of puerarin-7-o-fructoside, a derivative that possesses improved water solubility and antioxidant activity. a minor product puerarin- ... | 2014 | 24629266 |
| stability of microbiota facilitated by host immune regulation: informing probiotic strategies to manage amphibian disease. | microbial communities can augment host immune responses and probiotic therapies are under development to prevent or treat diseases of humans, crops, livestock, and wildlife including an emerging fungal disease of amphibians, chytridiomycosis. however, little is known about the stability of host-associated microbiota, or how the microbiota is structured by innate immune factors including antimicrobial peptides (amps) abundant in the skin secretions of many amphibians. thus, conservation medicine ... | 2014 | 24489847 |
| [screening of chlorobenzene-degrading bacterium and purification of its degradation enzyme]. | we screened a bacterial strain capable of degrading chlorobenzene, and purified the corresponding degradation enzyme. | 2013 | 23957149 |
| lysinibacillus tabacifolii sp. nov., a novel endophytic bacterium isolated from nicotiana tabacum leaves. | a gram-positive, catalase- and oxidase-positive, strictly aerobic, endospore-forming rod bacterium, designated k3514(t), was isolated from the leaves of nicotiana tabacum. the strain was able to grow at temperatures of 8-40°c, ph 5.0-10.0 and nacl concentrations of 0-7%. the predominant quinones (>30%) of this strain were mk-7(h2) and mk-7. phylogenetic analysis of 16s rrna gene sequence showed that strain k3514(t) was affiliated to the genus lysinibacillus, with its closest relatives being lysi ... | 2013 | 23812807 |
| total synthesis of spirobacillene a. | the first total synthesis of spirobacillene a, an indole alkaloid isolated from lysinibacillus fusiformis, is reported. a lewis acid mediated spirocyclization of an anisole derivative onto a tethered ynone was used as a key step, drawing inspiration from a potential biosynthesis of the natural product. | 2013 | 23786419 |
| impact of fe and ni/fe nanoparticles on biodegradation of phenol by the strain bacillus fusiformis (bfn) at various ph values. | the bacillus fusiformis (bfn) strain and its biodegradation of phenol when iron-based nanoparticles such as nanoscale zero-valent iron (nzvi) and ni/fe nanoparticles (ni/fe) were present at different ph values (6.0, 8.0, and 3.0) were investigated. the growth of bfn and its biodegradation of phenol accelerated in the presence of nzvi and ni/fe both at ph 8.0 and ph 6.0. the h2 generated by the corrosion of iron can be used as an electron donor and source of energy for growing bfn. however, only ... | 2013 | 23567735 |
| highly luminescent and biocompatible, l-citrulline-capped zns:mn nanocrystals for rapid screening of metal accumulating lysinibacillus fusiformis bacteria. | biocompatible and highly luminescent manganese doped zinc sulfide (zns:mn) nanocrystals of average particle size 10 nm have been synthesized by capping with a novel amino acid ligand, l-citrulline. though there are many reports on the bioimaging applications of nanostructured semiconductors, the present study focused on the detection of a special type of metal accumulating bacteria, lysinibacillus fusiformis. this bacterium has significant applications in the disposal of metal components from in ... | 2013 | 23494908 |
| production of a novel compound, 10,12-dihydroxystearic acid from ricinoleic acid by an oleate hydratase from lysinibacillus fusiformis. | a recombinant oleate hydratase from lysinibacillus fusiformis converted ricinoleic acid to a product, whose chemical structure was identified as the novel compound 10,12-dihydroxystearic acid by gas chromatograph/mass spectrometry, fourier transform infrared, and nuclear magnetic resonance analysis. the reaction conditions for the production of 10,12-dihydroxystearic acid were optimized as follows: ph 6.5, 30 °c, 15 g l(-1) ricinoleic acid, 9 mg ml(-1) of enzyme, and 4 % (v/v) methanol. under th ... | 2013 | 23377790 |
| spirobacillenes a and b, unusual spiro-cyclopentenones from lysinibacillus fusiformis kmc003. | two previously unreported spiro-cyclopentenones, spirobacillenes a (1) and b (2), were isolated from the 24 h broth culture of lysinibacillus fusiformis kmc003 derived from acidic coal-mine drainage. the structures of 1 and 2 were elucidated by analyses of the nmr, hrfabms, single-crystal x-ray diffraction crystallography, and circular dichroism (cd) spectral data. compound 1 possessed moderate inhibitory activity against the production of nitric oxide (no) and reactive oxygen species (ros). | 2012 | 22985468 |
| optimization of media components for chitinase production by chickpea rhizosphere associated lysinibacillus fusiformis b-cm18. | chitinase producing strain b-cm18 was isolated from chickpea rhizosphere and identified as lysinibacillus fusiformis b-cm18. it showed in vitro antifungal activity against a wide range of fungal plant pathogens and was found to produce several pgpr activities. further, a multivariate response surface methodology was used to evaluate the effects of different factors on chitinolytic activity and optimizing enzyme production. a central composite design was employed to achieve the highest chitinase ... | 2013 | 22733389 |
| biosequestration, transformation, and volatilization of mercury by lysinibacillus fusiformis isolated from industrial effluent. | in the present study, an efficient mercury-tolerant bacterial strain (rs-5) was isolated from heavy-metalcontaminated industrial effluent. under shake flask conditions, 97% of the supplemented mercuric chloride was sequestered by the biomass of rs-5 grown in a tryptone soy broth. the sequestered mercuric ions were transformed inside the bacterial cells, as an xrd analysis of the biomass confirmed the formation of mercurous chloride, which is only feasible following the reaction of the elemental ... | 2012 | 22561864 |
| lysinibacillus mangiferahumi sp. nov., a new bacterium producing nematicidal volatiles. | a gram-positive bacterium, designated m-gx18(t), was isolated from the rhizosphere soil of mango (guangxi province, china). the isolate produced nematicidal volatile compounds with activities against the root-knot nematode meloidogyne incognita. the organism was motile, catalase- and oxidase-positive, spore-forming and rod shaped. the predominant menaquinone was mk-7 and the major cellular fatty acid profiles were iso-c(15:0), anteiso-c(15:0) and iso-c(16:0). the dna g+c content was 38.9%. a phy ... | 2012 | 22367102 |
| lysinibacillus fusiformis m5 induces increased complexity in bacillus subtilis 168 colony biofilms via hypoxanthine. | in recent years, biofilms have become a central subject of research in the fields of microbiology, medicine, agriculture, or systems biology amongst others. the sociomicrobiology of multispecies biofilms, however, is still poorly understood. here, we report a screening system that allowed us to identify soil bacteria, which induce architectural changes in biofilm colonies when cocultured with b. subtilis we identified the soil bacterium lysinibacillus fusiformis m5 as inducer of wrinkle-formatio ... | 2017 | 28583948 |