| phenotypic differentiation of bifidobacteria of human and animal origins. | the phenotypes of 153 strains belonging or related to the genus bifidobacterium were studied. these organisms included 38 collection strains and 115 wild strains (41 strains of human origin, 56 strains of animal origin, and 18 strains obtained from rivers or sewage). our phenotypic analysis revealed seven main groups that were subdivided into 20 subgroups. seven subgroups contained no type or collection strain. among the human strains, the type strains of bifidobacterium pseudocatenulatum and b. ... | 1991 | 1742200 |
| molecular microbial analysis of bifidobacterium isolates from different environments by the species-specific amplified ribosomal dna restriction analysis (ardra). | one hundred and six isolates of the genus bifidobacterium, isolated from different environments (mainly gastrointestinal), were identified and classified taxonomically to species level by amplified ribosomal dna restriction analysis. two restriction endonucleases (sau3ai and bamhi) were chosen for aligning the 16s rrna sequences of 16 bifidobacterial species retrieved from various databases, to obtain species-specific restriction patterns. a rapid and accurate identification scheme was obtained ... | 2001 | 11451515 |
| identification, detection, and enumeration of human bifidobacterium species by pcr targeting the transaldolase gene. | methods that enabled the identification, detection, and enumeration of bifidobacterium species by pcr targeting the transaldolase gene were tested. bifidobacterial species isolated from the feces of human adults and babies were identified by pcr amplification of a 301-bp transaldolase gene sequence and comparison of the relative migrations of the dna fragments in denaturing gradient gel electrophoresis (dgge). two subtypes of bifidobacterium longum, five subtypes of bifidobacterium adolescentis, ... | 2002 | 11976117 |
| probiotic bifidobacteria protect mice from lethal infection with shiga toxin-producing escherichia coli o157:h7. | the anti-infectious activity of probiotic bifidobacteria against shiga toxin-producing escherichia coli (stec) o157:h7 was examined in a fatal mouse stec infection model. stable colonization of the murine intestines was achieved by the oral administration of bifidobacterium breve strain yakult (naturally resistant to streptomycin sulfate) as long as the mice were treated with streptomycin in their drinking water (5 mg/ml). the pathogenicity of stec infection, characterized by marked body weight ... | 2004 | 15039348 |
| bifidobacterial species differentially affect expression of cell surface markers and cytokines of dendritic cells harvested from cord blood. | the gut microbiota may be important in the postnatal development of the immune system and hence may influence the prevalence of atopic diseases. bifidobacteria are the most numerous bacteria in the guts of infants, and the presence or absence of certain species could be important in determining the geographic incidence of atopic diseases. we compared the fecal populations of bifidobacteria from children aged 25 to 35 days in ghana (which has a low prevalence of atopy), new zealand, and the unite ... | 2004 | 15242942 |
| antibiotic susceptibility of lactobacillus and bifidobacterium species from the human gastrointestinal tract. | one hundred and twenty-two strains of bifidobacterium and lactobacillus species have been tested against 12 antibiotics and two antibiotic mixtures by a commercial system (sensititre anaero3; treck diagnostic systems). the upper limits of some minimum inhibitory concentrations (mics) were completed on mrs agar plates by the nccls procedure. all strains were sensitive to chloramphenicol and imipenem and most of the strains were resistant to metronidazole. bifidobacteria isolates were susceptible ... | 2005 | 15902467 |
| genetic diversity of viable, injured, and dead fecal bacteria assessed by fluorescence-activated cell sorting and 16s rrna gene analysis. | a novel approach combining a flow cytometric in situ viability assay with 16s rrna gene analysis was used to study the relationship between diversity and activity of the fecal microbiota. simultaneous staining with propidium iodide (pi) and syto bc provided clear discrimination between intact cells (49%), injured or damaged cells (19%), and dead cells (32%). the three subpopulations were sorted and characterized by denaturing gradient gel electrophoresis (dgge) of 16s rrna gene amplicons obtaine ... | 2005 | 16085863 |
| conditions affecting cell surface properties of human intestinal bifidobacteria. | the cell surface properties of human intestinal bifidobacteria have been characterized for 30 strains isolated from a fecal sample. strain identification to the species level was obtained by restriction analysis of the amplified 16s rrna gene and confirmed by dna/dna reassociation experiments. the isolates were grouped in four genetically homogeneous clusters whose members belonged to bifidobacterium bifidum, bifidobacterium adolescentis, bifidobacterium longum and bifidobacterium pseudocatenula ... | 2005 | 16284927 |
| two families of rep-like genes that probably originated by interspecies recombination are represented in viral, plasmid, bacterial, and parasitic protozoan genomes. | two families of genes related to, and including, rolling circle replication initiator protein (rep) genes were defined by sequence similarity and by evidence of intergene family recombination. the rep genes of circoviruses were the best characterized members of the "recrep1 family." other members of the recrep1 family were rep-like genes found in the genomes of the canarypox virus, entamoeba histolytica, and giardia duodenalis and in a plasmid, p4m, from the gram-positive bacterium, bifidobacter ... | 2006 | 16531508 |
| inhibitory impact of bifidobacteria on the transfer of beta-lactam resistance among enterobacteriaceae in the gnotobiotic mouse digestive tract. | while looking for new means to limit the dissemination of antibiotic resistance, we evaluated the role of potentially probiotic bifidobacteria on the transfer of resistance genes between enterobacteria. transfers of bla genes encoding extended-spectrum beta-lactamases (shv-5 and ctx-m-15) were studied in the absence or presence of bifidobacteria. in vitro, transfer frequencies of these bla genes decreased significantly in the presence of three of five tested strains, i.e., bifidobacterium longum ... | 2007 | 17122392 |
| molecular analyses of the intestinal microbiota of chimpanzees in the wild and in captivity. | little information is available regarding the intestinal bacteria of chimpanzees in the wild, due to the technical difficulties of studying intestinal bacteria in the field. in this study, molecular-based bacterial analyses were performed to overcome this difficulty because polymerase chain reaction (pcr)-based methods, such as temperature gradient gel electrophoresis (tgge) and amplified ribosomal dna restriction analysis (ardra), of the bacterial 16s rrna gene can be applied to ethanol-fixed f ... | 2007 | 17177283 |
| bifidobacterium pseudocatenulatum is associated with atopic eczema: a nested case-control study investigating the fecal microbiota of infants. | exposure to specific bacterial bowel commensals may increase/reduce the risk of atopic diseases. | 2008 | 17900682 |
| safety evaluation of bifidobacterium pseudocatenulatum g4 as assessed in balb/c mice. | to assess the safety of bifidobacterium pseudocatenulatum g4 in balb/c mice that involves examination of bacterial translocation, changes in the internal organs and histology of the intestinal lining. | 2008 | 17944838 |
| in vitro evaluation of the probiotic properties of human intestinal bifidobacterium species and selection of new probiotic candidates. | the aim of this work was to identify and select new probiotic strains among majority intestinal bifidobacterial species from healthy spaniards. | 2008 | 18248372 |
| exopolysaccharides produced by intestinal bifidobacterium strains act as fermentable substrates for human intestinal bacteria. | eleven exopolysaccharides (eps) isolated from different human intestinal bifidobacterium strains were tested in fecal slurry batch cultures and compared with glucose and the prebiotic inulin for their abilities to act as fermentable substrates for intestinal bacteria. during incubation, the increases in levels of short-chain fatty acids (scfa) were considerably more pronounced in cultures with eps, glucose, and inulin than in controls without carbohydrates added, indicating that the substrates a ... | 2008 | 18539803 |
| improved cloning vectors for bifidobacteria, based on the bifidobacterium catenulatum pbc1 replicon. | this study reports the development of several cloning vectors for bifidobacteria based on the replicon of pbc1, a cryptic plasmid from bifidobacterium catenulatum l48 thought to replicate via the theta mode. these vectors, in which antibiotic resistance genes encoding either erythromycin or tetracycline resistance acted as selection markers, were able to replicate in a series of eight bifidobacterium species at frequencies ranging from 4.0 x 10(1) to 1.0 x 10(5) transformants microg(-1) but not ... | 2008 | 18539807 |
| effect of inulin on the human gut microbiota: stimulation of bifidobacterium adolescentis and faecalibacterium prausnitzii. | prebiotics are food ingredients that improve health by modulating the colonic microbiota. the bifidogenic effect of the prebiotic inulin is well established; however, it remains unclear which species of bifidobacterium are stimulated in vivo and whether bacterial groups other than lactic acid bacteria are affected by inulin consumption. changes in the faecal microbiota composition were examined by real-time pcr in twelve human volunteers after ingestion of inulin (10 g/d) for a 16-d period in co ... | 2009 | 18590586 |
| quantification of folic acid in human feces after administration of bifidobacterium probiotic strains. | folic acid, or vitamin b9, is involved in appropriate regulation of dna replication, synthesis of purines and deoxythymidine (dtmp), conversion of homocysteine to methionine, histidine catabolism, and correct differentiation of the neural tube during fetal organogenesis. folic acid from food sources is almost completely absorbed in the small intestine, mostly in the jejunum, and does not reach the large intestine. the administration of probiotic strains able to synthesize folates de novo and rel ... | 2008 | 18685499 |
| synbiotics growth optimization of bifidobacterium pseudocatenulatum g4 with prebiotics using a statistical methodology. | this study demonstrated the optimum growth of bifidobacterium pseudocatenulatum g4 with prebiotics via statistical model. | 2009 | 19054238 |
| exploring the diversity of the bifidobacterial population in the human intestinal tract. | although the health-promoting roles of bifidobacteria are widely accepted, the diversity of bifidobacteria among the human intestinal microbiota is still poorly understood. we performed a census of bifidobacterial populations from human intestinal mucosal and fecal samples by plating them on selective medium, coupled with molecular analysis of selected rrna gene sequences (16s rrna gene and internally transcribed spacer [its] 16s-23s spacer sequences) of isolated colonies. a total of 900 isolate ... | 2009 | 19168652 |
| intestinal bifidobacterium association in germ-free t cell receptor transgenic mice down-regulates dietary antigen-specific immune responses of the small intestine but enhances those of the large intestine. | bifidobacterium is a dominant bacterial species among commensals in the human intestine and is thought to have probiotic immunomodulatory effects. in this study, we investigated the effect of the association with bifidobacterium pseudocatenulatum jcm 7041 (bp) on dietary ovalbumin (ova)-specific immune responses using germ-free ova-specific t cell receptor transgenic mice (ova23-3 mice). we established germ-free ova23-3 mice, and then associated with bp (bif group) or without (cont group) and ad ... | 2009 | 19327545 |
| a tlr2 ligand suppresses allergic inflammatory reactions by acting directly on mast cells. | although much attention has been focused on the anti-allergic effects of probiotics, their mode of action is not fully understood. mast cells, which play a central role in inducing allergic inflammation, are potential targets of probiotics given the recent discovery that they express toll-like receptors (tlrs), the pattern recognition receptors for microbial components. in this study, we examined whether allergic reactions of mast cells are modulated by stimulation through tlr2. | 2009 | 19571568 |
| phytate degradation by human gut isolated bifidobacterium pseudocatenulatum atcc27919 and its probiotic potential. | the growing awareness of the relationship between diet and health has led to an increasing demand for food products that support health above and beyond providing basic nutrition. probiotics are live organisms present in foods, which yield health benefits related to their interactions with the gastrointestinal tract. phytases are a subgroup of phosphatases that catalyse the desphosphorylation of phytate, which reduces its negative impact on mineral bioavailability, and generates lower inositol p ... | 2009 | 19674804 |
| production of exopolysaccharides by lactobacillus and bifidobacterium strains of human origin, and metabolic activity of the producing bacteria in milk. | this work reports on the physicochemical characterization of 21 exopolysaccharides (eps) produced by lactobacillus and bifidobacterium strains isolated from human intestinal microbiota, as well as the growth and metabolic activity of the eps-producing strains in milk. the strains belong to the species lactobacillus casei, lactobacillus rhamnosus, lactobacillus plantarum, lactobacillus vaginalis, bifidobacterium animalis, bifidobacterium longum, and bifidobacterium pseudocatenulatum. the molar ma ... | 2009 | 19700676 |
| differentiation of bifidobacterium species using partial rna polymerase {beta}-subunit (rpob) gene sequences. | partial rna polymerase β-subunit gene (rpob) sequences (315 bp) were determined and used to differentiate the type strains of 23 species of the genus bifidobacterium. the sequences were compared with those of the partial hsp60 (604 bp) and 16s rrna genes (1475 or 1495 bp). the rpob gene sequences showed nucleotide sequence similarities ranging from 84.1 % to 99.0 %, while the similarities of the hsp60 sequences ranged from 78.5 % to 99.7 % and the 16s rrna gene sequence similarities ranged from ... | 2010 | 20061504 |
| amoxicillin treatment modifies the composition of bifidobacterium species in infant intestinal microbiota. | amoxicillin is a beta-lactam antibiotic largely used in childhood. however only few studies described its impact on composition of children gut microbiota, in particular on bifidobacterium populations considered as beneficial microorganisms. in this study, the impact on faecal bifidobacterium species of a seven-day amoxicillin treatment was quantitatively and qualitatively assessed in infants during an episode of acute respiratory infection. | 2010 | 20601031 |
| bifidobacterium kashiwanohense sp. nov., isolated from healthy infant faeces. | hm2-1 and hm2-2t strains were isolated from the faeces of a healthy infant and were characterised by determining their phenotypic and biochemical features and phylogenetic positions based on partial 16s rrna gene sequence analysis. they were gram-positive, obligately anaerobic, non-spore-forming, non-gas-producing, and catalase-negative non-motile rods. they did not show growth at 15 and 45°c in abcm broth, and their dna g+c content is in the range of 56-59 mol%. in enzyme activity tests, hm2-1 ... | 2010 | 21148680 |
| orally administered bifidobacterium triggers immune responses following capture by cd11c(+) cells in peyer's patches and cecal patches. | we have investigated the immunomodulatory mechanisms of bifidobacterium pseudocatenulatum jcm7041 (bp) as model of probiotics following oral administration to mice. this study was conducted with the aim of clarifying the mechanism of immunomodulation induced by oral administration of probiotic bacteria through elucidation of the detailed mechanism of transfer of orally administered bacterial cells within the body and the interaction between bacterial cells and cells of the immune tissues. we obs ... | 2011 | 21461945 |
| role of bifidobacteria in the activation of the lignan secoisolariciresinol diglucoside. | lignans are ubiquitous plant polyphenols, which have relevant health properties being the major phytoestrogens occurring in western diets. secoisolariciresinol (seco) is the major dietary lignan mostly found in plants as secoisolariciresinol diglucoside (sdg). to exert biological activity, sdg requires being deglycosylated to seco and transformed to enterodiol (ed) and enterolactone (el) by the intestinal microbes. the involvement of bifidobacteria in the transformation of lignans glucosides has ... | 2011 | 21614502 |
| changes of fecal bifidobacterium species in adult patients with hepatitis b virus-induced chronic liver disease. | the beneficial effects of bifidobacteria on health have been widely accepted. patients with chronic liver disease have varying degrees of intestinal microflora imbalance with a decrease of total bifidobacterial counts. since different properties have been attributed to different bifidobacterium species and there is no information available for the detailed changes in the genus bifidobacterium in patients with chronic liver disease heretofore, it is meaningful to investigate the structure of this ... | 2011 | 21814872 |
| in vitro binding of mutagenic heterocyclic aromatic amines by bifidobacterium pseudocatenulatum g4. | consumption of probiotics has been associated with decreased risk of colon cancer and reported to have antimutagenic/ anti-carcinogenic properties. one possible mechanism for this effect involves physical binding of the mutagenic compounds, such as heterocyclic amines (hcas), to the bacteria. therefore, the objective of this study was to examine the binding capacity of bifidobacterial strains of human origin on mutagenic heterocyclic amines which are suspected to play a role in human cancers. in ... | 2010 | 21831754 |
| fecal microbiota composition differs between children with β-cell autoimmunity and those without. | the role of the intestinal microbiota as a regulator of autoimmune diabetes in animal models is well-established, but data on human type 1 diabetes are tentative and based on studies including only a few study subjects. to exclude secondary effects of diabetes and hla risk genotype on gut microbiota, we compared the intestinal microbiota composition in children with at least two diabetes-associated autoantibodies (n = 18) with autoantibody-negative children matched for age, sex, early feeding hi ... | 2012 | 23274889 |
| the genome of bifidobacterium pseudocatenulatum ipla 36007, a human intestinal strain with isoflavone-activation activity. | bifidobacterium species, including bifidobacterium pseudocatenulatum, are among the dominant microbial populations of the human gastrointestinal tract. they are also major components of many commercial probiotic products. resident and transient bifidobacteria are thought to have several beneficial health effects. however, our knowledge of how these bacteria interact and communicate with host cells remains poor. this knowledge is essential for scientific support of their purported health benefits ... | 2014 | 25097668 |
| intestinal microbiota distinguish gout patients from healthy humans. | current blood-based approach for gout diagnosis can be of low sensitivity and hysteretic. here via a 68-member cohort of 33 healthy and 35 diseased individuals, we reported that the intestinal microbiota of gout patients are highly distinct from healthy individuals in both organismal and functional structures. in gout, bacteroides caccae and bacteroides xylanisolvens are enriched yet faecalibacterium prausnitzii and bifidobacterium pseudocatenulatum depleted. the established reference microbial ... | 2016 | 26852926 |
| measurements of intra- and extra-cellular 5-methyltetrahydrofolate indicate that bifidobacterium adolescentis dsm 20083(t) and bifidobacterium pseudocatenulatum dsm 20438(t) do not actively excrete 5-methyltetrahydrofolate in vitro. | certain intestinal bifidobacteria have the ability to synthesize folates. in vitro experiments revealed a high production, cellular accumulation, and release of reduced folate vitamers like 5-methyltetrahydrofolate and tetrahydrofolate in folate-free medium (ffm). however, it is still unclear to which extent synthesized folates are polyglutamylated and probably not available for transport, and if they are actively released by excretion. to address these questions, we characterized intra- and ext ... | 2017 | 28377750 |
| high purity galacto-oligosaccharides enhance specific bifidobacterium species and their metabolic activity in the mouse gut microbiome. | prebiotics are selectively fermented ingredients that result in specific changes in the composition and/or activity of the gastrointestinal microbiota, thus conferring benefit(s) upon the host health. the aim of this study was to evaluate the influence of a β(1-4)galacto-oligosaccharides (gos) formulation consisting of 90% pure gos (gos90), on the composition and activity of the mouse gut microbiota. germ-free mice were colonised with microbiota from four pathogen-free wt 129 mice donors (spf), ... | 2016 | 26839072 |
| antiviral activity of bifidobacterium adolescentis spm1605 against coxsackievirus b3. | bifidobacteria are considered one of the most beneficial probiotics and have been widely studied for their effects against specific pathogens. the present study investigated the antiviral activity of probiotics isolated from koreans against coxsackievirus b3 (cvb3). the effect of probiotic isolates against cvb3 was measured by the plaque assay and cellular toxicity of bifidobacteria in hela cells was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay. among 1 ... | 2014 | 26019554 |
| inulin-type fructans modulate intestinal bifidobacterium species populations and decrease fecal short-chain fatty acids in obese women. | inulin-type fructans (itf) prebiotics promote changes in the composition and activity of the gut microbiota. the aim of this study was to determine variations on fecal short chain fatty acids (scfa) concentration in obese women treated with itf and to explore associations between bifidobacterium species, scfa and host biological markers of metabolism. | 2015 | 24969566 |
| mother-to-infant transmission of intestinal bifidobacterial strains has an impact on the early development of vaginally delivered infant's microbiota. | bifidobacterium species are one of the major components of the infant's intestine microbiota. colonization with bifidobacteria in early infancy is suggested to be important for health in later life. however, information remains limited regarding the source of these microbes. here, we investigated whether specific strains of bifidobacteria in the maternal intestinal flora are transmitted to their infant's intestine. | 2013 | 24244304 |
| isolation of a bifidogenic peptide from the pepsin hydrolysate of bovine lactoferrin. | lactoferrin is an iron-binding glycoprotein found in the milk of most mammals for which various biological functions have been reported, such as antimicrobial activity and bifidogenic activity. in this study, we compared the bifidogenic activity of bovine lactoferrin (blf) and pepsin hydrolysate of blf (blfh), isolated bifidogenic peptide from blfh, and investigated the bifidogenic spectra of blf, blfh, and its active peptide against 42 bifidobacterial strains comprising nine species. against bi ... | 2013 | 23315725 |
| exploration of the genomic diversity and core genome of the bifidobacterium adolescentis phylogenetic group by means of a polyphasic approach. | in the current work, we describe genome diversity and core genome sequences among representatives of three bifidobacterial species, i.e., bifidobacterium adolescentis, bifidobacterium catenulatum, and bifidobacterium pseudocatenulatum, by employing a polyphasic approach involving analysis of 16s rrna gene and 16s-23s internal transcribed spacer (its) sequences, pulsed-field gel electrophoresis (pfge), and comparative genomic hybridization (cgh) assays. | 2013 | 23064340 |
| antagonistic activities of some bifidobacterium sp. strains isolated from resident infant gastrointestinal microbiota on gram-negative enteric pathogens. | the gastrointestinal microbiota contributes to the consolidation of the anti-infectious barrier against enteric pathogens. the purpose of this study was to investigate the influence of bifidobacterium sp. strains, recently isolated from infant gastrointestinal microbiota on the in vitro growth and virulence features expression of enteropathogenic bacterial strains. the antibacterial activity of twelve bifidobacterium sp. strains isolated from human feces was examined in vitro against a wide rang ... | 2016 | 26921694 |
| prebiotic-non-digestible oligosaccharides preference of probiotic bifidobacteria and antimicrobial activity against clostridium difficile. | bifidobacterium breve 46, bifidobacterium lactis 8:8 and bifidobacterium longum 6:18 and three reference strains b. breve ccug 24611, b. lactis jcm 10602, and bifidobacterium pseudocatenulatum jcm 1200 were examined for acid and bile tolerance, prebiotic utilization and antimicrobial activity against four clostridium difficile (cd) strains including the hypervirulent strain, pcr ribotype nap1/027. b. lactis 8:8 and b. lactis jcm 10602 exhibited a high tolerance in mrsc broth with ph 2.5 for 30 m ... | 2012 | 22940065 |
| bifidobacterium moukalabense sp. nov., isolated from the faeces of wild west lowland gorilla (gorilla gorilla gorilla). | gram-staining-positive anaerobic rods were isolated from the faeces of a wild lowland gorilla (gorilla gorilla gorilla) in moukalaba-doudou national park, gabon, and strain gg01(t) was taxonomically investigated. based on phylogenetic analyses and specific phenotypic characteristics, the strain belonged to the genus bifidobacterium. phylogenetic analysis of its 16s rrna gene sequence revealed that strain gg01(t) formed a single monophyletic cluster and had a distinct line of descent. based on 16 ... | 2014 | 24158945 |
| effects of feed additives on ileal mucosa-associated microbiota composition of broiler chickens. | the effects of dietary supplementation with 2 recently developed feed additives on the composition of the mucosa-associated microbiota of the ileum were studied in growing broiler chickens. a total of 48 male 1-d-old broiler chickens of the cobb 500 strain were distributed in 4 treatments with 2 replicates of 6 birds each. the 2 additives tested were a di-d-fructose dianhydride–enriched caramel (fc) and the garlic derivative propyl propane thiosulfonate (pts-o). dietary treatments were a control ... | 2015 | 26440010 |
| expression of bifidobacterial phytases in lactobacillus casei and their application in a food model of whole-grain sourdough bread. | phytases are enzymes capable of sequentially dephosphorylating phytic acid to products of lower chelating capacity and higher solubility, abolishing its inhibitory effect on intestinal mineral absorption. genetic constructions were made for expressing two phytases from bifidobacteria in lactobacillus casei under the control of a nisin-inducible promoter. l. casei was able of producing, exporting and anchoring to the cell wall the phytase of bifidobacterium pseudocatenulatum. the phytase from bif ... | 2016 | 26384212 |
| reduction of phytate in soy drink by fermentation with lactobacillus casei expressing phytases from bifidobacteria. | plant-based food products can be modified by fermentation to improve flavour and the concentration of some biologically active compounds, but also to increase the mineral availability by eliminating anti-nutrient substances such as phytates. the objective of this study was to develop a fermented soybean drink with improved nutritional quality and source of probiotic bacteria by including as starter for fermentation lactobacillus casei strains modified to produce phytase enzymes from bifidobacter ... | 2015 | 26003176 |
| preliminary selection for potential probiotic bifidobacterium isolated from subjects of different chinese ethnic groups and evaluation of their fermentation and storage characteristics in bovine milk. | a total of 29 strains of bifidobacterium were isolated from 18 samples of human feces in different ethnic minority regions of china. all isolates were identified as bifidobacterium longum (9 strains) and bifidobacterium pseudocatenulatum (20 strains) based on 16s rrna gene sequencing and phylogenetic analysis. these strains were preliminarily tested for their suitability to become probiotics by assessing their ability to survive adequately at low ph conditions and their tolerance of different co ... | 2013 | 24054292 |
| application of bifidobacterial phytases in infant cereals: effect on phytate contents and mineral dialyzability. | phytase activity was recently described in probiotic bifidobacterial strains, opening the possibilities for their use in foods, due to the generally regarded as safe/qualified presumption of safety status of these bacteria. two raw materials for infant cereals (multicereal and gluten-free) were examined by measuring the myo-inositol phosphates content and the in vitro ca, fe, and zn availability after a dephytinization process with purified phytases from bifidobacterium longum spp. infantis and ... | 2012 | 23151205 |
| hypocholesterolaemic effect of yoghurt containing bifidobacterium pseudocatenulatum g4 or bifidobacterium longum bb536. | the effect of a yoghurt supplement containing bifidobacterium pseudocatenulatum g4 or bifidobacterium longum bb536 on plasma lipids, lipid peroxidation and the faecal excretion of bile acids was examined in rats fed a cholesterol-enriched diet. after 8 weeks, the rats in the positive control (pc) group who were fed the cholesterol-enriched diet showed significant increases in plasma total cholesterol (tc), low-density lipoprotein (ldl) cholesterol, and malondialdehyde (mda). however, groups fed ... | 2012 | 22868099 |
| novel phytases from bifidobacterium pseudocatenulatum atcc 27919 and bifidobacterium longum subsp. infantis atcc 15697. | two novel phytases have been characterized from bifidobacterium pseudocatenulatum and bifidobacterium longum subsp. infantis. the enzymes belong to a new subclass within the histidine acid phytases, are highly specific for the hydrolysis of phytate, and render myo-inositol triphosphate as the final hydrolysis product. they represent the first phytases characterized from this group of probiotic microorganisms, opening the possibilities for their use in the processing of high-phytate-content foods ... | 2012 | 22582052 |
| genomic microdiversity of bifidobacterium pseudocatenulatum underlying differential strain-level responses to dietary carbohydrate intervention. | the genomic basis of the response to dietary intervention of human gut beneficial bacteria remains elusive, which hinders precise manipulation of the microbiota for human health. after receiving a dietary intervention enriched with nondigestible carbohydrates for 105 days, a genetically obese child with prader-willi syndrome lost 18.4% of his body weight and showed significant improvement in his bioclinical parameters. we obtained five isolates (c1, c15, c55, c62, and c95) of one of the most abu ... | 2017 | 28196965 |
| genome structure of the symbiont bifidobacterium pseudocatenulatum cect 7765 and gene expression profiling in response to lactulose-derived oligosaccharides. | bifidobacterium pseudocatenulatum cect 7765 was isolated from stools of a breast-fed infant. although, this strain is generally considered an adult-type bifidobacterial species, it has also been shown to have pre-clinical efficacy in obesity models. in order to understand the molecular basis of its adaptation to complex carbohydrates and improve its potential functionality, we have analyzed its genome and transcriptome, as well as its metabolic output when growing in galacto-oligosaccharides der ... | 2016 | 27199952 |
| bifidobacterium pseudocatenulatum cect7765 induces an m2 anti-inflammatory transition in macrophages from patients with cirrhosis. | patients with cirrhosis show recurrent access of bacterial products into the bloodstream inducing a multi-altered immunological status leading to relevant complications. we aimed at evaluating bifidobacterium pseudocatenulatum cect7765 effect on the host's macrophage function. | 2016 | 26334579 |
| bifidobacterium pseudocatenulatum cect 7765 reduces obesity-associated inflammation by restoring the lymphocyte-macrophage balance and gut microbiota structure in high-fat diet-fed mice. | the role of intestinal dysbiosis in obesity-associated systemic inflammation via the cross-talk with peripheral tissues is under debate. our objective was to decipher the mechanisms by which intervention in the gut ecosystem with a specific bifidobacterium strain reduces systemic inflammation and improves metabolic dysfunction in obese high-fat diet (hfd) fed mice. | 2015 | 26161548 |
| complete genome sequence of bifidobacterium pseudocatenulatum jcm 1200(t) isolated from infant feces. | bifidobacterium pseudocatenulatum jcm 1200(t) was isolated from infant feces. this paper is the first report demonstrating the fully sequenced and completely annotated genome of a b. pseudocatenulatum strain. | 2015 | 26133926 |
| myo-inositol hexakisphosphate degradation by bifidobacterium pseudocatenulatum atcc 27919 improves mineral availability of high fibre rye-wheat sour bread. | the goal of this investigation was to develop baking products using bifidobacterium pseudocatenulatum atcc27919, a phytase producer, as a starter in sourdough for the production of whole rye-wheat mixed bread. this bifidobacterium strain contributed to myo-inositol hexakisphosphate (phytate) hydrolysis, resulting in breads with higher mineral availability as was predicted by the phytate/mineral molar ratios, which remained below the inhibitory threshold values for ca and zn intestinal absorption ... | 2015 | 25704711 |
| bifidobacterium pseudocatenulatum cect7765 promotes a tlr2-dependent anti-inflammatory response in intestinal lymphocytes from mice with cirrhosis. | intestinal homeostasis plays an important role in bacteria-derived complications in cirrhosis. intestinal lymphocytes are responsible for immune effector functions and can be modulated by certain probiotics. we evaluate the interaction between bifidobacterium pseudocatenulatum cect7765 and intestinal lymphocytes in mice with cirrhosis. | 2016 | 25657013 |
| effect of alginate and chitosan on viability and release behavior of bifidobacterium pseudocatenulatum g4 in simulated gastrointestinal fluid. | the main aim of this study was to investigate the effect of different coating materials (i.e. na-alginate and chitosan) on the viability and release behavior of bifidobacterium pseudocatenulatum g4 in the simulated gastric fluid (sgf) and simulated intestinal fluid (sif). this study reports the viability of encapsulated b. pseudocatenulatum g4 coated using different alginate (2-4 g/100ml) and chitosan (0.2-0.8 g/100ml) concentrations. the results indicated that the highest concentration of algin ... | 2014 | 25037405 |
| optimization of milk-based medium for efficient cultivation of bifidobacterium pseudocatenulatum g4 using face-centered central composite-response surface methodology. | this study was undertaken to optimize skim milk and yeast extract concentration as a cultivation medium for optimal bifidobacteria pseudocatenulatum g4 (g4) biomass and β -galactosidase production as well as lactose and free amino nitrogen (fan) balance after cultivation period. optimization process in this study involved four steps: screening for significant factors using 2(3) full factorial design, steepest ascent, optimization using fccd-rsm, and verification. from screening steps, skim milk ... | 2014 | 24527457 |
| protective effect of bifidobacterium pseudocatenulatum cect7765 against induced bacterial antigen translocation in experimental cirrhosis. | intervention in the gut ecosystem is considered as a potential strategy to treat liver diseases and their complications. we have evaluated the effects of bifidobacterium pseudocatenulatum cect7765 on bacterial translocation and the liver status in experimental cirrhosis. | 2014 | 24267920 |
| hepatic molecular responses to bifidobacterium pseudocatenulatum cect 7765 in a mouse model of diet-induced obesity. | bifidobacterium pseudocatenulatum cect 7765 moderates body weight gain and metabolic parameters in high-fat diet-(hfd)-fed mice but, the mechanisms of action are not yet understood. to further understand the effects of this bacterial strain, we have investigated the molecular changes in the liver of mice fed a hfd and supplemented with the bacteria. | 2014 | 23831006 |
| bifidobacterium cect 7765 improves metabolic and immunological alterations associated with obesity in high-fat diet-fed mice. | to evaluate the effects of administration of bifidobacterium pseudocatenulatum cect 7765 on metabolic and immune alterations in obese mice. | 2013 | 23418126 |
| cloning and expression of synthetic genes encoding angiotensin-i converting enzyme (ace)-inhibitory bioactive peptides in bifidobacterium pseudocatenulatum. | a wide range of biopeptides potentially able to lower blood pressure through inhibition of the angiotensin-i converting enzyme (ace) is produced in fermented foods by proteolytic starter cultures. this work applies a procedure based on recombinant dna technologies for the synthesis and expression of three ace-inhibitory peptides using a probiotic cell factory. ace-inhibitory genes and their pro-active precursors were designed, synthesized by pcr, and cloned in escherichia coli; after which, they ... | 2013 | 23278337 |
| effect of feeding a selected combination of galacto-oligosaccharides and a strain of bifidobacterium pseudocatenulatum on the intestinal microbiota of cats. | to evaluate the growth kinetics of a strain of bifidobacterium pseudocatenulatum (bp) on 4 oligo- or polysaccharides and the effect of feeding a selected probiotic-prebiotic combination on intestinal microbiota in cats. | 2013 | 23270351 |
| viability and activity of bifidobacteria during refrigerated storage of yoghurt containing mangifera pajang fibrous polysaccharides. | the viability and activity of bifidobacterium pseudocatenulatum g4, b. longum bb 536 and yoghurt cultures (lactobacillus delbrueckii ssp. bulgaricus and streptococcus thermophilus) were studied in yoghurt containing 0.75% mangefira pajang fibrous polysaccharides (mpfp) and inulin. growth of probiotic organisms, their proteolytic activities, the production of short chain fatty acids (lactic, acetic and propionic) and the ph of the yoghurt samples were determined during refrigerated storage at 4 ° ... | 2012 | 23106104 |
| prebiotic effect of soluble fibres from modern and old durum-type wheat varieties on lactobacillus and bifidobacterium strains. | wheat grains are a rich source of dietary fibres, particularly in the western human diet. many of the health effects attributed to dietary fibres are believed to be related to their microbial fermentation in the gut. this study evaluated the ability of two potentially probiotic strains, lactobacillus plantarum l12 and bifidobacterium pseudocatenulatum b7003, to ferment soluble dietary fibres (sdfs) from modern and ancient durum-type wheat grains. | 2012 | 22298124 |