| phase variations in bifidobacterium animalis. | strains isolated from rabbit, chicken, and rat feces and from sewage and fermented milk products, all identified as bifidobacterium animalis, were found to show phase variations in colony appearance and in cellular morphology. the rate of transition in a switching system from opaque to transparent colonies and vice versa was determined. differences in protein components and in penicillin-binding proteins (pbps) of the cells from different colony types are shown. | 1992 | 1369190 |
| identification of bifidobacteria from fermented milk products. | six samples of fermented milk preparations were examined for the presence of bifidobacteria. identification was based on fermentation tests, genetic relatedness studies and electrophoretic analysis. contrary to label information, bifidobacterium animalis was the only species present. | 1992 | 1556961 |
| different electrophoretic patterns of cellular soluble proteins in bifidobacterium animalis. | twenty-nine strains of bifidobacterium animalis, a species found only in animal habitats, were studied. strains from known origins such as rat, rabbit and chicken feces and strains isolated from extrabody environments such as sewage and fermented milk products were examined. the intestinal origins of strains isolated from sewage and fermented milk products were determined by means of the comparison of electrophoretograms of cellular soluble proteins. unknown origins of strains were recognized as ... | 1992 | 1556962 |
| gastrointestinal flora of cotton rats. | the gastrointestinal (gi) flora of cotton rats was examined. no lactobacilli were detected in any part of the gi tract. anaerobes, including peptococcaceae, bacteroidaceae, bifidobacteria and eubacteria, were the predominant bacteria in the stomach, small intestine, caecum and faeces. aerobes and facultative anaerobes, including enterobacteriaceae and streptococci, were detected at low numbers and very low frequency of occurrence in all parts of the gi tract. sixty-one isolates of bifidobacteria ... | 1989 | 2657217 |
| characterization of fructose 6 phosphate phosphoketolases purified from bifidobacterium species. | fructose 6 phosphate phosphoketolases (f6ppks) were purified from bifidobacterium longum bb536, b. dentium atcc 27534, b. globosum atcc 25864, and bifidobacterium animalis atcc 25527. concerning ions (cu++, zn++, ca++, mg++, fe++, co++, mn++) and common enzyme inhibitors (fructose, ammonium sulfate, iodoacetate, and parachloromercuribenzoic acid), no difference appeared between the enzymes. cu++, parachloromercuribenzoic acid (pcmb), and mercuric acetate induced high enzymatic inhibition. the st ... | 1995 | 7767228 |
| differentiation of bifidobacteria by use of pulsed-field gel electrophoresis and polymerase chain reaction. | several different genomic fingerprints can be obtained from various commercially-important species of bifidobacterium using pulsed-field gel electrophoresis (pfge) following digestion of dna with xbai and spei. four different genomic finger printings were discernible for reference strains of bifidobacterium animalis, five for b. bifidum, three for b. breve, five for b. infantis and three for b. longum. standard commercially-available industrial strains of b. animalis are identical to the referen ... | 1996 | 8722183 |
| characterization of the plasmid pmb1 from bifidobacterium longum and its use for shuttle vector construction. | the nucleotide sequence of the 1847-bp bifidobacterium longum b2577 cryptic plasmid pmb1 was determined. the plasmid had a g+c content of 62.0%, and contained two open reading frames, orf1 and orf2, likely arranged in an operon. the proteins encoded by orf1 and orf2 show the highest degree of similarity with similarly arranged peptide sequences translated from corynebacterium glutamicum pxz10142 and mycobacterium fortuitum pal5000 plasmids. recombinant plasmids containing the pmb1 replicon were ... | 1996 | 8761732 |
| antiproliferative effect of fermented milk on the growth of a human breast cancer cell line. | in vivo and in vitro studies have shown an antitumor activity of lactobacilli in colon cancer, and some epidemiologic studies have indicated a reduced risk of breast cancer in women who consume fermented milk products. we studied the direct effect of milk fermented by five bacteria strains (bifidobacterium infantis, bifidobacterium bifidum, bifidobacterium animalis, lactobacillus acidophilus, and lactobacillus paracasei) on the growth of the mcf7 breast cancer cell line. our results showed a gro ... | 1997 | 9200156 |
| colonization of congenitally immunodeficient mice with probiotic bacteria. | we assessed the capacity of four probiotic bacteria (lactobacillus acidophilus, lactobacillus reuteri, lactobacillus casei gg, and bifidobacterium animalis) to colonize, infect, stimulate immune responses in, and affect the growth and survival of congenitally immunodeficient gnotobiotic beige-athymic (bg/bg-nu/nu) and beige-euthymic (bg/bg-nu/+) mice. the bacteria colonized and persisted, in pure culture, in the alimentary tracts of both mouse strains for the entire study period (12 weeks). alth ... | 1997 | 9234796 |
| digoxigenin-labeled deoxyribonucleic acid probes for the enumeration of bifidobacteria in fecal samples. | the numbers of bifidobacteria in fecal samples were specifically determined by colony hybridization with the mixture of digoxigenin-labeled dna probes that were prepared from whole chromosomal dna of bifidobacterium longum 6001 and bifidobacterium adolescentis 6003. these dna probes strongly hybridized with dna of b. longum, b. adolescentis, bifidobacterium breve, bifidobacterium suis, bifidobacterium infantis, bifidobacterium bifidum, bifidobacterium angulatum, and bifidobacterium animalis. det ... | 1997 | 9241587 |
| biotherapeutic effects of probiotic bacteria on candidiasis in immunodeficient mice. | four species of probiotic bacteria were assessed for their capacities to protect athymic bg/bg-nu/nu and euthymic bg/bg-nu/+ mice from mucosal and systemic candidiasis. each bacterial species and candida albicans colonized the gastrointestinal tracts of both strains of mice. the presence of probiotic bacteria (lactobacillus acidophilus, lactobacillus reuteri, lactobacillus casei gg, or bifidobacterium animalis) in the gastrointestinal tracts prolonged the survival of adult and neonatal bg/bg-nu/ ... | 1997 | 9317023 |
| conservation of the major cold shock protein in lactic acid bacteria. | primers designed from consensus regions of the major cold shock gene of different bacterial species were used in pcr amplification of lactic acid bacteria (lab). an appropriately-sized pcr product was obtained from lactococcus lactis subsp. lactis ll43-1 and mg1363; lactococcus lactis subsp. cremoris lc10-1, lc11-1, and lc12-1; streptococcus thermophilus st1-1; enterococcus faecalis ef1-1; lactobacillus acidophilus la1-1; lactobacillus helveticus lh1-1; pediococcus pentosaceus pp1-1; and bifidob ... | 1998 | 9767713 |
| sequence analysis of plasmid pkj50 from bifidobacterium longum. | the complete nucleotide sequence of a plasmid, pkj50, isolated from an intestinal bacterium, bifidobacterium longum kj, has been determined. the plasmid was analysed and found to be 4960 bp in size with a g+c content of 61.7 mol%. computer analysis of sequence data revealed three major orfs encoding putative proteins of 31.5 (orfi), 24.5 (orfii) and 38.6 kda (orfiii). orfi encodes a protein with a pi of 10.18 and shows relatively high amino acid sequence similarity (more than 60%) with several p ... | 1999 | 10217492 |
| bifidobacterium animalis protects intestine from damage induced by zinc deficiency in rats. | we investigated the potential beneficial effects of bifidobacterium animalis on intestinal damage using zinc-deficient (zd) rats as a model for intestinal alterations. the zd rats were fed diets containing 1 mg zn/kg for 20 (zd(20)) or 40 (zd(40)) d to induce damage that differed in severity. subgroups of these rats, the zd(20) + b and zd(40) + b groups, received a suspension of b. animalis (3.5 x 10(8) colony forming units) daily for the last 10 d. another subgroup, the zd(40) + b + 7 d group, ... | 1999 | 10573559 |
| a probiotic strain of l. acidophilus reduces dmh-induced large intestinal tumors in male sprague-dawley rats. | probiotic bacteria strains were examined for their influence on 1,2-dimethylhydrazine (dmh)-induced intestinal tumors in 100 male sprague-dawley rats. lactobacillus acidophilus (delvo pro la-1), lactobacillus rhamnosus (gg), bifidobacterium animalis (cscc1941), and streptococcus thermophilus (dd145) strains were examined for their influence when added as freeze-dried bacteria to an experimental diet based on a high-fat semipurified (ain-93) rodent diet. four bacterial treatments were compared: l ... | 1999 | 10693169 |
| a colony immunoblotting method for quantitative detection of a bifidobacterium animalis probiotic strain in human faeces. | a colony immunoblotting method has been developed to allow detection of the probiotic bifidobacterium animalis strain dn-173 010 in human faecal samples. rabbits were immunized with heat-killed dn-173 010 bacteria resulting in the production of an antiserum highly specific for bacteria belonging to bif. animalis species. of the 89 strains representative of 29 different bifidobacterial species tested, only the 15 strains of the bif. animalis species could be detected with the antiserum. in wester ... | 2000 | 10849178 |
| bifidobacterium lactis meile et al. 1997 is a subjective synonym of bifidobacterium animalis (mitsuoka 1969) scardovi and trovatelli 1974. | bifidobacterium lactis jcm 10602t (t = type strain) and bifidobacterium animalis jcm 1190t were found to be phenotypically similar. these strains were subjected to investigation of their genetic relationships. the 16s rrna sequence of b. animalis jcm 1190t was aligned with that of other bifidobacterium species. b. animalis and b. lactis were the most closely related species in the phylogenetic tree and showed a high similarity in sequences (98.8%). the levels of dna-dna hybridization between the ... | 2000 | 11128064 |
| molecular microbial analysis of bifidobacterium isolates from different environments by the species-specific amplified ribosomal dna restriction analysis (ardra). | one hundred and six isolates of the genus bifidobacterium, isolated from different environments (mainly gastrointestinal), were identified and classified taxonomically to species level by amplified ribosomal dna restriction analysis. two restriction endonucleases (sau3ai and bamhi) were chosen for aligning the 16s rrna sequences of 16 bifidobacterial species retrieved from various databases, to obtain species-specific restriction patterns. a rapid and accurate identification scheme was obtained ... | 2001 | 11451515 |
| new approach to phylogenetic analysis of the genus bifidobacterium based on partial hsp60 gene sequences. | the partial 60 kda heat-shock protein (hsp60) genes of 36 bifidobacterium strains representing 30 different bifidobacterium species and subspecies and of the type strain of gardnerella vaginalis were cloned and sequenced using a pair of universal degenerate hsp60 pcr primers. the hsp60 dna sequence similarities were determined for the taxa at various ranks as follows: 99.4-100% within the same species, 96% at the subspecies level, and 73-96% (mean 85%) at the interspecies level (and 98% in the c ... | 2001 | 11594590 |
| bifidobacterium animalis strain dn-173 010 shortens the colonic transit time in healthy women: a double-blind, randomized, controlled study. | a previous study has suggested that bifidobacterium animalis dn-173 010 shortens the colonic transit time in women. | 2002 | 11876714 |
| effects of dairy products on heterocyclic aromatic amine-induced rat colon carcinogenesis. | heterocyclic aromatic amines (haa) are initiating agents of colon carcinogenesis in animals and are suspected in the aetiology of human colon cancer. in the context of prevention, it seems interesting to test possible protective compounds, such as fermented milk, against haa food carcinogens. male f344 rats were used in a model of haa-induced colon carcinogenesis. the haa, 2-amino-3-methylimidazo[4,5-f]quinoline (iq), 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (meiq) and 2-amino-1-methyl-6-phen ... | 2002 | 11895863 |
| production of oligosaccharides in yogurt containing bifidobacteria and yogurt cultures. | yogurts were prepared by using yogurt cultures combined to mixed cultures of bifidobacteria (bifidobacterium animalis, bifidobacterium bifidum, bifidobacterium breve, bifidobacterium infantis, and bifidobacterium longum) and by adding a preincubation step (1.5 h at 50 degrees c) with bifidobacteria to the conventional method of manufacture in order to produce oligosaccharides. the survival of bifidobacteria was drastically affected during storage of yogurts, except for products containing b. ani ... | 2002 | 12086039 |
| synthesis and utilisation of folate by yoghurt starter cultures and probiotic bacteria. | thirty-two bacterial isolates from species commonly used in yoghurts and fermented milks were examined for their ability to synthesise or utilise folate during fermentation of skim milk. the organisms examined included the traditional yoghurt starter cultures, lactobacillus delbrueckii subsp. bulgaricus and streptococcus thermophilus, and probiotic lactobacilli, bifidobacteria, and enterococcus faecium. folate was synthesised by s. thermophilus, bifidobacteria, and e. faecium. s. thermophilus wa ... | 2003 | 12423923 |
| rapid identification, differentiation, and proposed new taxonomic classification of bifidobacterium lactis. | identification of bifidobacterium lactis and bifidobacterium animalis is problematic because of phenotypic and genetic homogeneities and has raised the question of whether they belong to one unique taxon. analysis of the 16s-23s internally transcribed spacer region of b. lactis dsm10140(t), b. animalis atcc 25527(t), and six potential b. lactis strains suggested two distinct clusters. two specific 16s-23s spacer rrna gene-targeted primers have been developed for specific detection of b. animalis ... | 2002 | 12450873 |
| specific growth rate of bifidobacteria cultured on different sugars. | the ability of six bifidobacterial strains (3 of human origin and 3 isolates from fermented milk products) to utilize glucose, lactose, melezitose, sucrose, raffinose, and stachyose was determined. dairy-related bifidobacterial strains were identified as bifidobacterium animalis (2 strains) or as b. pseudolongum (1 strain). human strains included b. longum (2 strains) and b. breve (1 strain). all strains fermented lactose, sucrose, raffinose, and stachyose. melezitose was utilized only by b. lon ... | 2002 | 12503390 |
| interaction of bifidobacteria with the gut and their influence in the immune function. | bifidobacteria are predominant in the lumen of the large intestine and confer various health benefits on the host. they are also used in the preparation of new fermented milks (bioyogurts) or added to conventional yogurt to generate probiotic effects. the colonization of the gut by bacteria tends to be host specific due partly to the way in which bacteria adhere to the intestinal wall. using a homologous strain of bifidobacterium animalis in an experimental mouse model, we analyzed by immunofluo ... | 2003 | 12847910 |
| growth and metabolism of selected strains of probiotic bacteria in milk. | growth and metabolism of five probiotic strains with well-documented health effects were studied in ultra-high temperature (uht) treated milk, supplemented with 0.5% (w/v) tryptone or 0.75% (w/v) fructose. the probiotic strains were lactobacillus acidophilus la5, lb. acidophilus 1748, lactobacillus rhamnosus gg, lactobacillus reuteri sd 2112 and bifidobacterium animalis bb12. fermentation was followed for 72 h at 37 degrees c and the samples were analysed for ph, log cfu ml(-1), volatile compoun ... | 2003 | 12927703 |
| stability of recombinant plasmids on the continuous culture of bifidobacterium animalis atcc 27536. | bifidobacterium animalis atcc 27536 represents among bifidobacteria a host-model for cloning experiments. the segregational and structural stabilities of a family of cloning vectors with different molecular weights but sharing a common core were studied in continuous fermentation of the hosting b. animalis without selective pressure. the rate of plasmid loss (r) and the specific growth rate difference (delta mu) between plasmid-free and plasmid-carrying cells were calculated for each plasmid and ... | 2003 | 12966570 |
| comparative sequence analysis of the tuf and reca genes and restriction fragment length polymorphism of the internal transcribed spacer region sequences supply additional tools for discriminating bifidobacterium lactis from bifidobacterium animalis. | the relationship between bifidobacterium lactis and bifidobacterium animalis was examined by comparative analysis of tuf and reca gene sequences and by restriction fragment length polymorphism analysis of their internal 16s-23s transcribed spacer region sequences. the bifidobacterial strains investigated could be divided into two distinct groups within a single species based on the tuf, reca, and 16s-23s spacer region sequence analysis. therefore, all strains of b. lactis and b. animalis could b ... | 2003 | 14660406 |
| short fractions of oligofructose are preferentially metabolized by bifidobacterium animalis dn-173 010. | the growth of bifidobacterium animalis dn-173 010 on different energy sources was studied through small- and large-scale fermentations. growth on both more common energy sources (glucose, fructose, galactose, lactose, and sucrose) and inulin-type fructans was examined. high-performance liquid chromatography analysis was used to investigate the kinetics. gas chromatography was used to determine the fructan degradation during the fermentation process. b. animalis dn-173 010 was unable to grow on a ... | 2004 | 15066781 |
| bifidobacterium lactis dsm 10140: identification of the atp (atpbefhagdc) operon and analysis of its genetic structure, characteristics, and phylogeny. | the atp operon is highly conserved among eubacteria, and it has been considered a molecular marker as an alternative to the 16s rrna gene. pcr primers were designed from the consensus sequences of the atpd gene to amplify partial atpd sequences from 12 bifidobacterium species and nine lactobacillus species. all pcr products were sequenced and aligned with other atpd sequences retrieved from public databases. genes encoding the subunits of the f(1)f(0)-atpase of bifidobacterium lactis dsm 10140 ( ... | 2004 | 15128574 |
| h+-atpase activity in bifidobacterium with special reference to acid tolerance. | the acid tolerance of 17 strains of nine species of bifidobacteria was compared using brief exposures to acidic conditions (ph 2-5). in addition, because it has been hypothesized that the acid tolerance of bifidobacteria depends on h+-atpase activity, the activity of this enzyme in various strains and species was compared. in general, the acid tolerance of bifidobacteria was found to be weak, with the exception of bifidobacterium lactis and bifidobacterium animalis. high numbers of all strains o ... | 2004 | 15135587 |
| effects of feeding a probiotic preparation (sim) containing inulin on the severity of colitis and on the composition of the intestinal microflora in hla-b27 transgenic rats. | an overly aggressive immune response to the intestinal microflora in a genetically susceptible host background has been implicated in the pathogenesis of inflammatory bowel diseases. we measured the impact of a probiotic preparation (sim) containing inulin on the severity of colitis and on intestinal microflora profiles of hla-b27-beta(2)-microglobulin transgenic (tg) rats. sim is a mixture of lactobacilli, bifidobacteria, and inulin. two-month-old tg rats received either sim or water. control t ... | 2004 | 15138186 |
| polyphasic taxonomic analysis of bifidobacterium animalis and bifidobacterium lactis reveals relatedness at the subspecies level: reclassification of bifidobacterium animalis as bifidobacterium animalis subsp. animalis subsp. nov. and bifidobacterium lactis as bifidobacterium animalis subsp. lactis subsp. nov. | the taxonomic standing of bifidobacterium lactis and bifidobacterium animalis was investigated using a polyphasic approach. sixteen representatives of both taxa were found to be phenotypically similar and shared more than 70% dna-dna relatedness (76-100%), which reinforces the conclusions of previous studies in which b. lactis and b. animalis were considered to be one single species. however, the results of protein profiling, box-pcr fingerprinting, fluorescent amplified fragment length polymorp ... | 2004 | 15280282 |
| bifidobacterium animalis strain dn-173 010 hydrolyses bile salts in the gastrointestinal tract of pigs. | bile salt hydrolase (bsh) activity is widespread among ingested bifidobacteria and lactobacilli. it is sometimes considered to be beneficial because of its putative lowering effect on cholesterol absorption and sometimes considered to be deleterious because it may compromise normal fat absorption and even promote the formation of secondary cytotoxic bile acids by the resident intestinal flora. however, the true hydrolysis of bile salts in vivo by ingested living bacteria remains unexplored. the ... | 2004 | 15743005 |
| a dynamic model that simulates the human upper gastrointestinal tract for the study of probiotics. | a dynamic model of the human upper gastrointestinal (gi) tract was designed to better simulate conditions of ingestion and digestion, by including a food matrix as part of the model design. the dynamic model consisted of two reactors maintained at 37 degrees c, one simulating stomach conditions and the other simulating duodenum conditions. the model was tested by comparing survival of bacteria isolated from humans (bifidobacterium infantis, lactobacillus johnsonii, lactobacillus rhamnosus, and l ... | 2005 | 15808363 |
| application of a microplate scale fluorochrome staining assay for the assessment of viability of probiotic preparations. | cell viability in probiotic preparations is traditionally assessed by the plate count technique. additionally, fluorescent staining combined with epifluorescence microscopy or flow cytometry has been developed for the viability assessment, but the currently available assays are either laborious or require highly sophisticated equipment. the aim of this study was to investigate the applicability of a microplate scale fluorochrome assay for predicting the cell state of freeze-dried lactobacillus r ... | 2005 | 15823392 |
| increasing ursodeoxycholic acid in the enterohepatic circulation of pigs through the administration of living bacteria. | we investigated the feasibility of increasing ursodeoxycholic acid (udca) in the enterohepatic circulation of pigs by administering living bacteria capable of epimerising endogenous amidated chenodeoxycholic acid (cdca) to udca. we first demonstrated that combining bifidobacterium animalis dn-173 010, as a bile salt-hydrolysing bacterium, and clostridium absonum atcc 27555, as a cdca to udca epimerising bacterium, led to the efficient epimerisation of glyco- and tauro-cdca in vitro, with respect ... | 2005 | 15946407 |
| analysis of the large bowel microbiota of colitic mice using pcr/dgge. | to test combined polymerase chain reaction amplification of 16s rrna gene sequences and denaturing gradient gel electrophoresis (pcr/dgge) as an analytical method to investigate the composition of the large bowel microbiota of mice during the development of colitis. | 2005 | 15960751 |
| culture-dependent and culture-independent qualitative analysis of probiotic products claimed to contain bifidobacteria. | a total of 58 probiotic products obtained worldwide, which were claimed to contain bifidobacterium strains (including 22 yoghurts, 5 dairy fruit drinks, 28 food supplements and 3 pharmaceutical preparations) were investigated in parallel using a culture-dependent and a culture-independent approach. three isolation media previously reported as selective for bifidobacterium were evaluated for their suitability in the quality analysis of these products. subsequently, possible bifidobacterial coloni ... | 2005 | 15992621 |
| acquisition of bile salt resistance promotes antibiotic susceptibility changes in bifidobacterium. | the effect of acquired resistance to bile on the antimicrobial sensitivity of two bifidobacterium strains (bifidobacterium animalis ipla4549 and bifidobacterium longum nizo b667) was studied. the mics of 23 different antibiotics belonging to the most clinically important groups were determined by using the etest method, which comprises nonporous plastic strips calibrated with a predefined gradient of antibiotic concentrations covering 15 twofold dilutions. the strains were sensitive to most anti ... | 2005 | 16161694 |
| review article: bifidobacteria as probiotic agents -- physiological effects and clinical benefits. | bifidobacteria, naturally present in the dominant colonic microbiota, represent up to 25% of the cultivable faecal bacteria in adults and 80% in infants. as probiotic agents, bifidobacteria have been studied for their efficacy in the prevention and treatment of a broad spectrum of animal and/or human gastrointestinal disorders, such as colonic transit disorders, intestinal infections, and colonic adenomas and cancer. the aim of this review is to focus on the gastrointestinal effects of bifidobac ... | 2005 | 16167966 |
| a bile salt-resistant derivative of bifidobacterium animalis has an altered fermentation pattern when grown on glucose and maltose. | the growth of bifidobacterium animalis subsp. lactis ipla 4549 and its derivative with acquired resistance to bile, b. animalis subsp. lactis 4549dox, was evaluated in batch cultures with glucose or the glucose disaccharide maltose as the main carbon source. the acquisition of bile salt resistance caused a change in growth pattern for both sugars, which mainly resulted in a preferential use of maltose compared to glucose, whereas the mother strain used both carbohydrates in a similar way. high-p ... | 2005 | 16269682 |
| effects of infant formula containing a mixture of galacto- and fructo-oligosaccharides or viable bifidobacterium animalis on the intestinal microflora during the first 4 months of life. | adding prebiotics or probiotics to infant formula to improve the intestinal flora of formula-fed infants is considered to be a major innovation. several companies have brought relevant formulations onto the market. however, comparative data on the effects of pre- and probiotics on the intestinal microflora of infants are not available. the present study aimed to compare the effects of infant formula containing a mixture of galacto- and fructo-oligosaccharides or viable bifidobacterium animalis o ... | 2005 | 16277782 |
| influence of fermentation time, cryoprotectant and neutralization of cell concentrate on freeze-drying survival, storage stability, and acid and bile exposure of bifidobacterium animalis ssp. lactis cells produced without milk-based ingredients. | to investigate the stability of bifidobacterium animalis ssp. lactis vtt e-012010 (=bb-12) during freeze-drying, storage and acid and bile exposure. the effect of harvesting time and composition and ph of the cryoprotectant on the survival was evaluated. the procedure was performed by using a milk-free culture medium and cryoprotectants to produce cells for nonmilk-based applications. | 2005 | 16313405 |
| enzymatic ability of bifidobacterium animalis subsp. lactis to hydrolyze milk proteins: identification and characterization of endopeptidase o. | the proteolytic system of bifidobacterium animalis subsp. lactis was analyzed, and an intracellular endopeptidase (pepo) was identified and characterized. this work reports the first complete cloning, purification, and characterization of a proteolytic enzyme in bifidobacterium spp. aminopeptidase activities (general aminopeptidases, proline iminopeptidase, x-prolyl dipeptidylaminopeptidase) found in cell extracts of b. animalis subsp. lactis were higher for cells that had been grown in a milk-b ... | 2005 | 16332835 |
| modulation of rat cecal microbiota by administration of raffinose and encapsulated bifidobacterium breve. | to investigate the effects of administration of raffinose and encapsulated bifidobacterium breve jcm 1192t cells on the rat cecal microbiota, in a preclinical synbiotic study groups of male wkah/hkm slc rats were fed for 3 weeks with four different test diets: basal diet (group bd), basal diet supplemented with raffinose (group raf), basal diet supplemented with encapsulated b. breve (group cb), and basal diet supplemented with both raffinose and encapsulated b. breve (group rcb). the bacterial ... | 2006 | 16391119 |
| determination of survival, identity and stress resistance of probiotic bifidobacteria in bio-yoghurts. | to determine the level of bifidobacteria in bio-yoghurts in the uk, identify the species, and compare the resistance of common bifidobacterium spp. to acidity and oxidative stress. | 2006 | 16478503 |
| faecal siga secretion in infants fed on pre- or probiotic infant formula. | secretory immunoglobulin a (siga) plays an important role in the defence of the gastrointestinal tract. the level of faecal siga antibody is associated with increased neutralization and clearance of viruses. formula-fed infants who lack the transfer of protective maternal siga from breast milk may benefit from strategies to support maturation of humoral immunity and endogenous production of siga. we aimed at studying the effects of standard, prebiotic and probiotic infant formulas on the faecal ... | 2006 | 16618363 |
| bifidobacterium species isolated from animal feces and from beef and pork meat. | bifidobacteria were isolated from 122 of 145 samples of animal feces (from cattle, swine, sheep, goats, horses, rabbits, chickens, geese, and pigeons) from farms in france and austria and from 92 of 955 production and processing chain samples of beef and pork (obtained at slaughter, cutting, and retail). bacterial strains were identified to species by phenotypic numerical classification based on api 50ch and id 32a tests and dna-dna hybridization. bifidobacterium pseudolongum was present in 81% ... | 2006 | 16629032 |
| probiotic treatment of collagenous colitis: a randomized, double-blind, placebo-controlled trial with lactobacillus acidophilus and bifidobacterium animalis subsp. lactis. | probiotic treatment may be effective in diseases involving gut microflora and intestinal inflammation. in collagenous colitis (cc), a potential pathogenic role of the gut microflora has been proposed. the effect of probiotic treatment in cc is unknown. our aim was to investigate the clinical effect of treatment with lactobacillus acidophilus la-5 and bifidobacterium animalis subsp. lactis bb-12 (ab-cap-10) in patients with cc. | 2006 | 16670529 |
| development of a competitive exclusion product for poultry meeting the regulatory requirements for registration in the european union. | competitive exclusion treatment is able to increase the pathogen colonization resistance of day-old chicks by applying probiotic bacteria stabilizing the indigenous microflora. in order to develop a safe microbial feed additive, various bacterial strains were isolated out of the gastrointestinal tract of healthy chickens. one hundred twenty-one representatives were selected based on differences in whole-cell protein patterns and screened for antagonistic properties. five effective strains (pedio ... | 2006 | 16676374 |
| antimicrobial susceptibility of bifidobacterium strains from humans, animals and probiotic products. | the aim of this study was to assess the antimicrobial susceptibility of a taxonomically diverse set of bifidobacterium strains to different classes of antimicrobial agents using a recently described medium. | 2006 | 16698847 |
| manufacturing of fermented goat milk with a mixed starter culture of bifidobacterium animalis and lactobacillus acidophilus in a controlled bioreactor. | this work was undertaken to study the feasibility and the characteristics of a fermented product made of goat milk, using a mixed starter culture of bifidobacterium animalis and lactobacillus acidophilus under controlled conditions, and to determine their survival in the fermented milk during refrigerated storage. | 2006 | 16706898 |
| dose-response study of probiotic bacteria bifidobacterium animalis subsp lactis bb-12 and lactobacillus paracasei subsp paracasei crl-341 in healthy young adults. | this study was performed to investigate the dose-response effects of supplementation with bifidobacterium animalis subsp lactis (bb-12) and lactobacillus paracasei subsp paracasei (crl-431) on blood lipids, recovery from feces and bowel habits. changes of the fecal microflora was analyzed in the 10(10) cfu/day probiotic and placebo group. | 2006 | 16721394 |
| probiotic bacteria bifidobacterium animalis mb5 and lactobacillus rhamnosus gg protect intestinal caco-2 cells from the inflammation-associated response induced by enterotoxigenic escherichia coli k88. | probiotic bacteria may provide protection against intestinal damage induced by pathogens, but the underlying mechanisms are still largely unknown. we investigated whether bifidobacterium animalis mb5 and lactobacillus rhamnosus gg (lgg) protected intestinal caco-2 cells from the inflammation-associated response induced by enterotoxigenic escherichia coli (etec) k88, by inhibiting pathogen attachment to the cells, which is the first step of etec pathogenicity, and regulating neutrophil recruitmen ... | 2006 | 16768842 |
| anti-inflammatory metabolite production in the gut from the consumption of probiotic yogurt containing bifidobacterium animalis subsp. lactis lkm512. | there is little evidence for a relationship between probiotic metabolites and host cytokine production. we investigated in the present study the possibility that anti-inflammatory metabolites can be produced in the gut by lkm512 yogurt consumption by using murine macrophage-like j774.1 cells and extracts prepared from the feces of elderly volunteers. these volunteers' acute inflammation had been inhibited by lkm512 yogurt consumption in a previous test. the tumor necrosis factor (tnf)-alpha prod ... | 2006 | 16794305 |
| immunomodulating potential of supplementation with probiotics: a dose-response study in healthy young adults. | certain probiotic microorganisms have been found beneficial in the treatment of immune-related diseases and may also affect immune function in healthy people. intervention studies of probiotics in healthy humans are urgently required. here, the immunomodulating potential of bifidobacterium animalis ssp. lactis (bb-12) and lactobacillus paracasei ssp. paracasei (crl-431) was studied in a double-blind placebo-controlled parallel dose-response trial (n=71) based on five randomly assigned groups of ... | 2006 | 16872374 |
| kinetic analysis of bifidobacterial metabolism reveals a minor role for succinic acid in the regeneration of nad+ through its growth-associated production. | several strains belonging to the genus bifidobacterium were tested to determine their abilities to produce succinic acid. bifidobacterium longum strain bb536 and bifidobacterium animalis subsp. lactis strain bb 12 were kinetically analyzed in detail using in vitro fermentations to obtain more insight into the metabolism and production of succinic acid by bifidobacteria. changes in end product formation in strains of bifidobacterium could be related to the specific rate of sugar consumption. when ... | 2006 | 16885266 |
| exopolysaccharides produced by probiotic strains modify the adhesion of probiotics and enteropathogens to human intestinal mucus. | exopolysaccharides (epss) are exocellular polymers present in the surface of many bacteria, including lactobacillus and bifidobacterium. the genome sequence of several strains revealed the presence of eps-encoding genes. however, the physiological role that epss play in the bacterial ecology still remains uncertain. in this study, we have assessed the effect of epss produced by lactobacillus rhamnosus gg, bifidobacterium longum nb667, and bifidobacterium animalis ipla-r1 on the adhesion of probi ... | 2006 | 16924934 |
| genetically engineered bifidobacterium animalis expressing the salmonella flagellin gene for the mucosal immunization in a mouse model. | a critical component of the host defense against enteric infections is the immunological response of the mucosal membrane, a major starting point of infectious disease, such as typhoid fever. the mucosal immune system consists of an integrated network of lymphoid tissues, mucous membrane-associated cells, and effector molecules. in the present study, we developed a recombinant bifidobacterium animalis (b. animalis) genetically modified with the salmonella flagellin gene for mucosal immunization ... | 2006 | 16958059 |
| the f1f0-atpase of bifidobacterium animalis is involved in bile tolerance. | adaptation and tolerance to bile stress are important factors for the survival of bifidobacteria in the intestinal tract. bifidobacterium animalis is a probiotic microorganism which has been largely applied in fermented dairy foods due to its technological properties and its health-promoting effects for humans. the effect of the presence of bile on the activity and expression of f(1)f(0)-atpase, the pool of atp and the intracellular ph of b. animalis ipla 4549 and its mutant with acquired resist ... | 2006 | 16958763 |
| effect of a probiotic food as an adjuvant to triple therapy for eradication of helicobacter pylori infection in children. | current recommendations for treatment of helicobacter pylori infection include a proton pump inhibitor in combination with two antibiotics. we evaluated the potential activity of a probiotic food as an adjuvant to antibiotic triple therapy for eradication of h. pylori infection in children from buenos aires, argentina. | 2006 | 16978844 |
| different utilization of glucose and raffinose in bifidobacterium breve and bifidobacterium animalis. | growth of 2 strains of bifidobacterium breve and 4 strains of bifidobacterium animalis was evaluated with glucose, raffinose or a mixture of glucose and raffinose as substrates. all strains of b. animalis and one strain of b. breve grew more slowly on glucose than on raffinose or the mixture of glucose and raffinose; one strain of b. breve grew more rapidly on the mixture of glucose and raffinose than on raffinose while 2 strains of b. animalis grew faster on raffinose than on the mixture of raf ... | 2006 | 17007436 |
| evaluation of real-time pcr targeting the 16s rrna and reca genes for the enumeration of bifidobacteria in probiotic products. | the application of real-time pcr targeting the multicopy 16s rrna gene and the single copy reca gene was evaluated for the enumeration of bifidobacteria in 29 probiotic products claimed to contain these organisms. both assays relied on the use of genus-specific primers and the non-specific sybr green i chemistry. for both applications, the calibration curve was constructed using the type strain of bifidobacterium animalis subsp. lactis. upon correction with a factor corresponding to the 16s rrna ... | 2007 | 17088006 |
| identification and differentiation of lactobacillus, streptococcus and bifidobacterium species in fermented milk products with bifidobacteria. | the aim of this study was to identify and discriminate bacteria contained in commercial fermented milks with bifidobacteria by the use of amplified ribosomal dna restriction analysis (ardra) and randomly amplified polymorphic dna (rapd) techniques. ardra of the 16s rdna gene and rapd were performed on 13 lactobacillus strains, 13 streptococcus and 13 bifidobacterium strains isolated from commercial fermented milk. lactobacillus delbrueckii, streptococcus thermophilus and bifidobacterium animalis ... | 2007 | 17112712 |
| antibiotic resistance of lactic acid bacteria and bifidobacterium spp. isolated from dairy and pharmaceutical products. | the outlines of antibiotic resistance of some probiotic microorganisms were studied. this study was conducted with the double purpose of verifying their ability to survive if they are taken simultaneously with an antibiotic therapy and to increase the selective properties of suitable media for the isolation of samples containing mixed bacterial populations. we isolated from commercial dairy and pharmaceutical products, 34 strains declared as probiotics, belonging to the genera bifidobacterium an ... | 2007 | 17198739 |
| tetracycline susceptibility of the ingested lactobacillus acidophilus lach-5 and bifidobacterium animalis subsp. lactis bb-12 strains during antibiotic/probiotic intervention. | we investigated the effects of oral therapy with doxycycline, a tetracycline group antibiotic, on the gastrointestinal (gi) survival and tetracycline susceptibility of probiotic strains lactobacillus acidophilus lach-5 and bifidobacterium animalis subsp. lactis bb-12. in addition, the influence of doxycycline therapy on the diversity of the predominant faecal microbiota was evaluated by polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge). faecal samples from the antibiot ... | 2007 | 17207972 |
| lkm512 yogurt consumption improves the intestinal environment and induces the t-helper type 1 cytokine in adult patients with intractable atopic dermatitis. | in atopic dermatitis (ad) patients, the intestinal mucosal barrier function is weakened, permiting frequent invasion by antigens. polyamines and short-chain fatty acids (scfa) produced by intestinal bacteria are involved in the promotion of intestinal mucosal barrier functions. | 2007 | 17359386 |
| differential analysis of protein expression of bifidobacterium grown on different carbohydrates. | we observed recently that colonic fermentation of lactose might be a major factor in the pathophysiology of lactose intolerance. proteomic techniques could be helpful in interpreting the metabolic pathways of lactose fermentation. the objective of this study was to explore proteomic methodologies for studying bacterial lactose metabolism that can be used to detect and identify proteins associated with the onset of intolerance symptoms. differential expression of cytoplasmic proteins of bifidobac ... | 2007 | 17397953 |
| innovative metabolic pathway design for efficient l-glutamate production by suppressing co2 emission. | in the pathway of l-glutamic acid (l-glu) biosynthesis in corynebacterium glutamicum, 1 mol of l-glu is synthesized from 1 mol of glucose at a cost of 1 mol of carbon dioxide (co(2)), with a maximum theoretical yield of 81.7% by weight. we have designed an innovative pathway for efficient l-glu production employing phosphoketolase (pkt) to bypass the co(2)-releasing pyruvate dehydrogenase reaction, thereby increasing the maximum theoretical yield of l-glu from glucose to up to 98.0% by weight (1 ... | 2007 | 17434430 |
| lactobacillus acidophilus 74-2 and bifidobacterium animalis subsp lactis dgcc 420 modulate unspecific cellular immune response in healthy adults. | it was determined whether a combination of lactobacillus acidophilus (l. acidophilus) 74-2 and bifidobacterium animalis subsp lactis dgcc 420 (b. lactis 420) affect the faecal microbiota as well as immunological parameters and blood lipids in healthy adults. | 2008 | 17440520 |
| myo-inositol hexakisphosphate degradation by bifidobacterium infantis atcc 15697. | the ability of five strains of different bifidobacterial species (bifidobacterium animalis, b. bifidum, b. infantis, b. longum, b. pseudolongum) isolated from human and animal gut to degrade myo-inositol hexaphosphate or phytic acid (insp(6)) has been evaluated. the disappearance of phytate and the generation of lower myo-inositol phosphates were determined in a complex medium in which phytic acid was the only source of phosphorus. bifidobacterium infantis atcc 15697 showed the highest level of ... | 2007 | 17462768 |
| selected prebiotics support the growth of probiotic mono-cultures in vitro. | the aim of the present study was to identify suitable prebiotics for synergistic combinations with representative probiotics from the lactobacilli and bifidobacterium genera. eleven different types of commercially available carbohydrates and compounds were screened in basal media for their effects on the growth of the probiotic cultures lactobacillus acidophilus lafti l10 (l10), bifidobacterium animalis lactis lafti b94 (b94) and lactobacillus casei lafti l26 (l26). growth of these probiotics wa ... | 2007 | 17544732 |
| microscopic colitis. | as the diagnosis of microscopic colitis (mc) is made on the basis of histologic criteria, it is crucial to render an accurate microscopic interpretation. features include 20 or more lymphocytes per 100 epithelial cells, mixed lamina propria inflammatory infiltrate, and preservation of crypt architecture for both lymphocytic and collagenous colitis (cc). cc is further characterized by a collagen band at least 10 mum thick. although the pathogenesis of mc is poorly understood, medication-induced t ... | 2007 | 17547861 |
| postnatal development of bacterial population in the gastrointestinal tract of calves. | bacterial 16s rdna from fecal samples of two calves were amplified by pcr and analyzed by denaturing gradient gel electrophoresis; selected bands were sequenced. escherichia coli and bifidobacterium animalis were the initial colonizers, followed by species closely related to the genera bacteroides, clostridium and faecalibacterium. change of diet was connected with shifts of bacterial population and with the occurrence of many bacterial species that have not been cultured up to now. the diet cha ... | 2007 | 17571805 |
| breast milk: a source of bifidobacteria for infant gut development and maturation? | the establishment of gut microbiota is a stepwise process contributing to gut development and maturation of the immune system. aberrant gut microbiota at an early age may predispose to disease later in life. breast-fed infants harbor a characteristic intestinal microbiota dominated by bifidobacteria. | 2007 | 17596738 |
| effect of a fermented milk containing bifidobacterium animalis dn-173 010 on the health-related quality of life and symptoms in irritable bowel syndrome in adults in primary care: a multicentre, randomized, double-blind, controlled trial. | health-related quality of life (hrqol) has been rarely evaluated as a primary endpoint in the assessment of the effect of probiotics on the irritable bowel syndrome (ibs). | 2007 | 17635382 |
| cell envelope changes in bifidobacterium animalis ssp. lactis as a response to bile. | bifidobacterium animalis ssp. lactis is a probiotic frequently used as adjunct culture in fermented dairy products. in order to ensure its proper function at the intestinal level, this bacterium has to be tolerant to physiological concentrations of bile. this study examined the influence of bile on the fatty acid composition and the membrane characteristics of b. animalis ipla 4549 and its mutant with acquired resistance to bile, b. animalis 4549dox. bile adaptation triggers in b. animalis 4549d ... | 2007 | 17651391 |
| a comparison of changes in the transformation of isoflavones in soymilk using varying concentrations of exogenous and probiotic-derived endogenous beta-glucosidases. | to compare endogenous and exogenous beta-glucosidases for the hydrolysis of the predominant isoflavone glucosides in soymilk in order to improve the biological activity. | 2007 | 17714393 |
| adaptation and response of bifidobacterium animalis subsp. lactis to bile: a proteomic and physiological approach. | bile salts are natural detergents that facilitate the digestion and absorption of the hydrophobic components of the diet. however, their amphiphilic nature makes them very inhibitory for bacteria and strongly influences bacterial survival in the gastrointestinal tract. adaptation to and tolerance of bile stress is therefore crucial for the persistence of bacteria in the human colonic niche. bifidobacterium animalis subsp. lactis, a probiotic bacterium with documented health benefits, is applied ... | 2007 | 17827318 |
| safety assessment of dairy microorganisms: propionibacterium and bifidobacterium. | the genera propionibacterium and bifidobacterium are clustered in the class actinobacteria and form the anaerobic branch of coryneform bacteria. the dairy propionibacteria comprising four species p. freudenreichii, p. acidipropionici, p. jensenii and p. thoenii are industrially important as starter cultures in hard-cheese ripening and recently also as protective bio-preservatives and probiotics. these four species are considered as safe whereas cutaneous propionibacterium species (also named "ac ... | 2008 | 17889391 |
| endogenous beta-glucosidase and beta-galactosidase activities from selected probiotic micro-organisms and their role in isoflavone biotransformation in soymilk. | to compare endogenous beta-glucosidases and beta-galactosidases for hydrolysis of the predominant isoflavone glycosides into isoflavone aglycones in order to improve biological activity of soymilk. | 2007 | 17897193 |
| clinical trial: multispecies probiotic supplementation alleviates the symptoms of irritable bowel syndrome and stabilizes intestinal microbiota. | irritable bowel syndrome is the most common diagnosis in gastroenterology. trials suggest certain probiotics to be beneficial. | 2008 | 17919270 |
| effects of yogurt and bifidobacteria supplementation on the colonic microbiota in lactose-intolerant subjects. | colonic metabolism of lactose may play a role in lactose intolerance. we investigated whether a 2-week supplementation of bifidobacterium longum (in capsules) and a yogurt enriched with bifidobacterium animalis could modify the composition and metabolic activities of the colonic microbiota in 11 chinese lactose-intolerant subjects. | 2008 | 17927751 |
| biological and genetic classification of canine intestinal lactic acid bacteria and bifidobacteria. | to investigate the distribution of lactic acid bacteria (lab) inhabiting canine intestines, a total of 374 gram-positive lab and bifidobacteria (bf) isolated from large intestinal contents in 36 dogs were classified and identified by phenotypic and genetic analyses. based on cell morphological sizes, these isolates were divided into seven biotypes containing the genera lactobacillus, bifidobacterium, enterococcus, and streptococcus. the lab and bf isolates were classified into 38 chemotypes base ... | 2007 | 17951981 |
| survival of bifidobacterium animalis dn-173 010 in the faecal microbiota after administration in lyophilised form or in fermented product - a randomised study in healthy adults. | the survival of bifidobacterium animalis strain dn-173 010 was assessed after its ingestion in a fermented product or in a lyophilised form. twelve healthy subjects were included in a randomised, open study with 2 parallel groups. the composition and activities of the faecal microbiota were monitored before (10-day baseline step), during (1-week product administration step) and after (10-day follow-up step) the ingestion of 1 of the 2 products. a colony immunoblotting method, fluorescent in situ ... | 2008 | 17957120 |
| effect of a probiotic mixed culture on texture profile and sensory performance of minas fresh cheese in comparison with the traditional products. | the effect of a mixed probiotic culture on instrumental texture, and on sensorial and related properties of minas fresh cheese during refrigerated storage was investigated. three cheese-making trials were prepared: t1, with the traditional type o starter culture (lactococcus lactis subsp. lactis + l. lactis subsp. cremoris), t2 with only lactic acid and t3, with lactic acid and the probiotic abt culture (lactobacillus acidophilus la-5 + bifidobacterium animalis bb-12 + streptococcus thermophilus ... | 2007 | 17992983 |
| biotransformation of isoflavone glycosides by bifidobacterium animalis in soymilk supplemented with skim milk powder. | two probiotic strains, bifidobacterium animalis a and b, were used for the biotransformation of isoflavone glycosides in soymilk prepared from soy protein isolate (spi) supplemented with skim milk powder (smp) (ssmp). unsupplemented soymilk (usm) and reconstituted skim milk powder (rsmp) were used as controls. the numbers of viable microorganisms in these products were enumerated. lactose and isoflavone contents were quantified using high-performance liquid chromatography (hplc). our results sho ... | 2007 | 17995612 |
| effects of beta-glucan addition to a probiotic containing yogurt. | this study investigated the effects of addition of beta-glucan from 2 different cereal sources (oat and barley) on growth and metabolic activity of bifidobacterium animalis ssp. lactis (bb-12) as determined by plating on a selective medium in yogurt during prolonged cold storage. these yogurt batches were compared to unsupplemented and inulin supplemented controls. all batches were also assessed for syneresis. oat beta-glucan addition resulted in improved probiotic viability and stability compar ... | 2007 | 17995639 |
| effects of fructooligosaccharide and whey protein concentrate on the viability of starter culture in reduced-fat probiotic yogurt during storage. | viability of yogurt starter cultures and bifidobacterium animalis was assessed during 28 d storage in reduced-fat yogurts containing 1.5% milk fat supplemented with 1.5% fructooligosaccharide or whey protein concentrate. these properties were examined in comparison with control yogurts containing 1.5% and 3% milk fat and no supplement. although fructooligosaccharide improved the viability of streptococcus thermophilus, lactobacillus delbrueckii subs. bulgaricus, and bifidobacterium animalis, the ... | 2007 | 17995644 |
| the immune system in healthy adults and patients with atopic dermatitis seems to be affected differently by a probiotic intervention. | probiotic bacteria are proposed to alleviate atopic dermatitis (ad) in infants. there are few indications about the effect of probiotics on ad in adults. | 2008 | 18028460 |
| detoxification of patulin and ochratoxin a, two abundant mycotoxins, by lactic acid bacteria. | aim of the present study was to investigate the detoxification of two abundant mycotoxins, namely ochratoxin a (ota) and patulin (pat) which are frequently found in human foods, by lactic acid bacteria. the removal of the two mycotoxins from liquid medium by thirty different lab strains was analyzed in a screening trial by the use of hplc coupled with uv- or fluorescence detection. two highly effective strains were identified; lactobacillus acidophilus vm 20 caused a decrease of ota by > or = 95 ... | 2008 | 18061329 |
| utilization of resistant starch of native tapioca, corn and waxy corn starches and their retrograded preparations by bifidobacterium. | anaerobic fermentation of native starches from tapioca, normal and waxy corn, and their laboratory modified preparations, by selected bifidobacterium strains (bifidobacterium pseudolongum ksi9, bifidobacterium breve kn14, and bifidobacterium animalis ks20a1) was carried out under in vitro conditions. the growth and acidifying properties of bifidobacteria and utilization of resistant starches were determined in relation to glucose in the control sample. the preparations obtained from normal and w ... | 2008 | 18097846 |
| bifidobacterium microbiota and parameters of immune function in elderly subjects. | faecal and serum samples were collected over a period of 6 months from 55 institutionalized elderly subjects, who were enrolled in a double-blind placebo-controlled study. participants were randomized in one of the three treatment groups: intervention (two probiotic bifidobacterium longum strains: 2c and 46), placebo and commercial control (bifidobacterium lactis bb-12). the faecal bifidobacterium microbiota was characterized by genus and species-specific pcr. serum levels of the cytokines il-10 ... | 2008 | 18336547 |
| resistance determinant erm(x) is borne by transposon tn5432 in bifidobacterium thermophilum and bifidobacterium animalis subsp. lactis. | the erm(x) gene from erythromycin- and clindamycin-resistant bifidobacterium strains was characterised by polymerase chain reaction and sequence analysis, including flanking regions. results suggest that the resistance determinant was part of transposon tn5432 that has been described in several opportunistic pathogens such as corynebacterium striatum and propionibacterium acnes. | 2008 | 18378122 |
| probiotic intervention has strain-specific anti-inflammatory effects in healthy adults. | to evaluate the effects of three potentially anti-inflammatory probiotic bacteria from three different genera on immune variables in healthy adults in a clinical setting based on previous in vitro characterization of cytokine responses. | 2008 | 18395902 |
| genetic analysis of a bile salt hydrolase in bifidobacterium animalis subsp. lactis kl612. | to clone, sequence and characterize a new bile salt hydrolase from a bile tolerant strain of bifidobacterium animalis ssp. lactis kl612, and further analysis of the bsh promoter and an operon-like structure containing the bsh gene in the genus bifidobacterium. | 2008 | 18444998 |
| effects of inulin and oligofructose on the rheological characteristics and probiotic culture survival in low-fat probiotic ice cream. | the effects of supplementation of oligofructose or inulin on the rheological characteristics and survival of lactobacillus acidophilus la-5 and bifidobacterium animalis bb-12 in low-fat ice cream stored at -18 degrees c for 90 d were studied. addition of oligofructose or inulin to ice cream mix significantly increased apparent viscosity and overrun and developed the melting properties in ice cream during storage (p < 0.05). however, the highest increase in firmness, the lowest change in melting ... | 2008 | 18460135 |
| [the probiotic yogurt activia shortens intestinal transit, but has not been shown to promote defecation]. | activia is a yogurt product containing the probiotic bacterium bifidobacterium animalis dn-173 010. five clinical trials have been carried out. four of these show that dairy products containing this bacterium shorten intestinal transit in volunteers. however, except in a subgroup of 19 out of 267 patients in one study, no significant effect of activia was reported on the frequency, quantity or consistency of stools. in its marketing in the netherlands, the company that produces activia, danone, ... | 2008 | 18461886 |