| corallotaenia minutia (freze 1965) (cestoda: proteocephalidea) from brown bullhead from western new york. | | 1976 | 1003274 |
| antibodies against enteric bacteria in brown bullhead catfish (ictalurus nebulosus, lesueur) inhabiting contaminated waters. | brown bullhead catfish were collected from sewage- and acid mine waste-polluted waters in an attempt to detect antibodies against human enteric bacteria in their sera and to investigate the association of antibody response with environmental conditions. agglutination antigens prepared from isolates obtained from water collected at the same locations as the fish habitats were used to demonstrate such antibodies. the results showed large percentages of reactive sera for common isolates such as esc ... | 1975 | 1099987 |
| tumors in fish caught in polluted waters: possible explanations. | the largest excess in tumor incidence due to pollution (616%) was observed in the fish species: ictalurus nebulosis (the brown bullhead). extensive analysis of waters on the fox river in illinois disclosed a heavy chemical and physical organic and inorganic materials far exceeding state and federal minimal safety standards. additional tests showed the presence of numerous human viral agents in the fox river. certain chemicals in the water appear to be potential carcinogens for fish. two types of ... | 1975 | 1174315 |
| pilum pilum gen. et sp. n. (acanthocephala: echinorhynchidae) from freshwater fishes of the southeastern united states. | pilum pilum gen. et sp. n. is described from the warmouth, lepomis gulosus (cuvier); brown bullhead, ictalurus nebulosus (lesueur); yellow bullhead, i. natalis (lesueur); redbreast sunfish, l. auritus (linnaeus); bluegill, l. macrochirus rafinesque; spotted sunfish, l. punctatus (valenciennes); and redfin pickerel, esox americanus (gmelin), from the alabama river drainage, brown bullhead from the mobile bay drainage in alabama, and pirate perch, aphredoderus sayanus gilliams, from an atlantic co ... | 1976 | 1255353 |
| adrenergic modulation of ca2+ homeostasis in isolated fish hepatocytes. | isolated hepatocytes of the american eel (anguilla rostrata lesueur) and brown bullhead (ictaluras nebulosus) have been used to characterize the effects of alpha- and beta-adrenergic agonists (epinephrine, phenylephrine, isoproterenol) and antagonists (phentolamine, propranolol) on calcium flux (influx, efflux) and cytosolic free calcium concentrations ([ca2+]i). bullhead hepatocytes have higher influx but lower efflux of ca2+ than eel hepatocytes, which may relate to the primary source for chan ... | 1992 | 1478443 |
| metabolism of benzo[a]pyrene and persistence of dna adducts in the brown bullhead (ictalurus nebulosus). | 1. the in vitro metabolism of [3h]benzo[a]pyrene (bp) and [14c]benzo[a]pyrene-7,8-dihydrodiol (bp-7,8-diol) by liver of brown bullhead (ictalurus nebulosus) was characterized, as was the formation and persistence of bp-dna adducts in vivo. 2. compared to rat liver microsomes, bullhead liver microsomes produced relatively larger amounts of bp-7,8-diol (predominantly the [-] enantiomer) and smaller amounts of of bp-7,8-diol (predominantly the [-] enantiomer) and smaller amounts of bp-4,5-diol. 3. ... | 1991 | 1677858 |
| biochemical and serological characterization of carnobacterium spp. isolated from farmed and natural populations of striped bass and catfish. | a comparative analysis of the phenotypic and serological properties of carnobacterium strains associated with mortalities of cultured striped bass and channel catfish and the properties of isolates from wild brown bullhead catfish in the chesapeake bay area in maryland was conducted. all of the strains were gram-positive, facultatively anaerobic, nonmotile, non-spore-forming rods occurring singly or in short chains. they did not produce cytochrome oxidase or catalase, did not reduce nitrate, fai ... | 1991 | 1781676 |
| metabolic response of teleost hepatocytes to glucagon-like peptide and glucagon. | salmon glucagon-like peptide (glp), bovine glucagon (b-glucagon) and anglerfish glucagon (af-glucagon), all activate glucose production in teleost hepatocytes through gluconeogenesis and glycogenolysis, but notable species differences exist in their respective effectiveness. in trout hepatocytes, gluconeogenesis appears to be the main target of hormone action. in eel cells, sampled in november, glycogenolysis was activated threefold, while gluconeogenesis was increased by 12% only. in march, gly ... | 1990 | 2166124 |
| comparative metabolism of benzo[f]quinoline by liver microsomes from brown bullheads and rats. | 1. liver microsomes from rats were considerably more active in metabolizing benzo[f]quinoline (b f q) than those from brown bullheads (ictalurus nebulosus). 2. the main b f q metabolites formed by both rat and brown bullhead liver microsomes were qualitatively similar and included b f q-7,8-dihydrodiol, b f q-9,10-dihydrodiol, b f q-n-oxide, 7-hydroxy b f q, and 9-hydroxy b f q. 3. the liver microsomes from control brown bullheads and rats metabolized b f q primarily at the 7,8-and 9,10-position ... | 1989 | 2572380 |
| in vitro effect of acyclovir and other antiherpetic compounds on the replication of channel catfish virus. | the antiviral effect of acyclovir (acv; 9-(2-hydroxyethoxymethyl)guanine) on the replication of channel catfish virus (ccv), a poikilothermic herpesvirus, in brown bullhead cells (bb) was studied in vitro. acyclovir at concentrations of 2 microm and 10 microm produced a 95 to 99% reduction in plaque numbers, respectively. at 10 microm acv did not affect the growth of uninfected bb cells which could even be subcultured for four passages in the presence of the inhibitor. to be effective acv had to ... | 1985 | 2932997 |
| metabolism and dna adduct formation of benzo[a]pyrene and 7,12-dimethylbenz[a]anthracene in fish cell lines in culture. | the metabolic activation of the carcinogens benzo[a]pyrene (bp) and 7,12-dimethylbenz[a]anthracene (dmba) was examined in cell lines derived from bluegill fry (bf-2), rainbow trout (rtg-2) and brown bullhead (bb). all three cell lines metabolized bp (0.5 microgram/ml medium) almost completely to water-soluble metabolites within 120 h, but the maximum amount of bp bound to dna ranged from only 5 pmol/mg dna in the bf-2 cells to 17 in the bb cells and 44 in the rtg-2 cells. the major bp-dna adduct ... | 1987 | 3115618 |
| analysis of the secretions of the subcommissural organs of several vertebrate species by use of fluorescent lectins. | the glycoprotein secretions of the subcommissural organ were analyzed with the use of nine fluorescent lectins, specific to different sugar moieties. after exposure to concanavalin a a bright fluorescence was observed in the ependymal cells of the subcommissural organs of all vertebrates studied (lampetra planeri, ameiurus nebulosus, bufo bufo, lacerta vivipara, gallus gallus, rattus norvegicus, ovis aries). the fluorescence is abolished by the competitive sugar, alpha-d-mannopyranosyl. the inte ... | 1985 | 3919951 |
| growth of brown bullhead (bb) and other fish cell lines on microcarriers and the production of channel catfish virus (ccv). | the growth of brown bullhead (bb) fish cells and other poikilothermic cell lines on four commercially available brands of microcarrier beads was evaluated. three of the microcarriers carry a positive surface charge, while the fourth carries a negative surface charge. the four microcarriers vary in their capacity to support the growth of the bb cells and other poikilothermic cell lines tested. the polyacrylamide beads by bio-rad and the polystyrene beads by nunc were found to best support the gro ... | 1985 | 3980665 |
| morphological survey of teleost organs important in carcinogenesis with attention to fixation. | key features of the gross and microscopic anatomy of teleost liver, digestive tract, kidney, and skin were reviewed from the world literature. illustrations of the above and new findings were obtained from light as well as scanning and transmission electron micrographs of tissues from 7 freshwater fish species: alosa sapidissima, american shad; esox lucius, northern pike; lepomis macrochirus, bluegill sunfish; pimephales promelas, fathead minnow; stizostedion vitreum, walleye; oryzias latipes, j ... | 1984 | 6087142 |
| characterization of benzo[a]pyrene metabolites formed by 3-methylcholanthrene-induced goldfish, black bullhead and brown bullhead. | metabolites of bap formed by 3-mc-induced goldfish (carassius auratus), black bullhead (ictalurus melas), brown bullhead (ictalurus nebulus) and rat have been separated by hplc. the main metabolites in both bullhead species and the rat were 3-hydroxy-bap and bap 9,10- and 7,8-dihydrodiols, whereas bap 1,6- and 3,6-diones and 9- and 3-hydroxy-bap predominated in the goldfish. induced rat formed over 10 times as many total metabolites as the fish. no induction was observed in the 3-mc-treated brow ... | 1984 | 6149861 |
| [roentgen microanalysis demonstration of zinc in the gustatory system of the bullhead, ameiurus nebulosus (teleostei)]. | the trace metal zinc (zn) seems to be essential for the normal functioning of the gustatory sense. in this study it was tried to localize zn within the peripheral and central parts of the bullhead's gustatory system by the use of scanning electron microscopy and x-ray microanalysis. in freeze dried preparations of the bullhead's barbel taste buds (and the taste buds of rabbits) zn is found in randomly distributed granules, which cannot be related to distinct taste bud regions. furthermore, zn oc ... | 1983 | 6412304 |
| characterization of immunoglobulins from the brown bullhead (ictalurus nebulosus) produced against a naturally occurring bacterial pathogen, aeromonas hydrophila. | the primary immune response in the brown bullhead (ictalurus nebulosus) to intramuscularly injected, chloroform-killed aeromonas hydrophila reached an agglutination titer of 128 in 3 wk, and the response to the bacteria plus adjuvant reached a titer of 512 in 5 wk. anti-aeromonas hydrophila antibodies from brown bullheads were excluded from sephadex g-200 and had an immunoelectrophoretic migration pattern toward the cathode. cleavage of the macromolecule by 2-me reduction and iodoacetamide alkyl ... | 1983 | 6642044 |
| catfish vitellogenin and its messenger rna are smaller than their chicken and xenopus counterparts. | injection of male bullheads (ameiurus nebulosus) with estradiol induced the production of a major serum phosphoprotein of molecular weight 145,000. this protein was immunoprecipitable by antisera raised against lipovitellin from bullhead eggs and was absent from the serum of control males. production of this serum protein coincided with changes in the liver mrna population, which suggested that estradiol had induced the synthesis of additional mrna sequences in the high-frequency class. agarose ... | 1980 | 7213633 |
| channel catfish virus: comparative replication and sensitivity of cell lines from channel catfish ovary and the brown bullhead. | a cell line derived from channel catfish ovary tissue was compared with the brown bullhead (bb) cell line for their respective abilities to replicate and detect channel catfish virus (ccv). the channel catfish ovary cell line (cco) produced cytopathic effects (cpe) more rapidly and detected ccv at higher dilutions than did the bb cell line. production of ccv was more rapid in cco cells than in bb cells, but the peak titers of the two lines were not significantly different. the cco cell line was ... | 1980 | 7411751 |
| a demonstration of human beta-2-microglobulin specific association with the surface of teleostean cells. | purified human beta-2 microglobulin (beta 2m) and a human beta 2m fluorochrome conjugate were used in exchange reactions to demonstrate that beta 2m associates with a teleostean cell surface protein. beta 2m exchange among brown bullhead, channel catfish, fathead minnow, and rainbow trout cells lines was detected by using either radioimmunoassay or flow cytometry. evidence that beta 2m binds specifically with the surface of teleostean cells and possibly associates with an expressed class i mhc h ... | 1994 | 7768315 |
| influence of dt diaphorase on quinone-mediated genotoxicity in human and fish cell lines. | the influence of the quinone-reducing enzyme, dt diaphorase [nad(p)h: (quinone acceptor) oxidoreductase], on the genotoxicity of quinones was examined in two cell lines, namely a human hepatoma cell line, hepg2 and a brown bullhead fibroblast cell line, bb. the quinone-reductive characteristics of these two cell lines were examined using an acetylated cytochrome c reduction assay for enzymatic reductase activity. subsequently, the influence of dt diaphorase on the genotoxicity of two model quino ... | 1996 | 8657209 |
| phase i and ii enzymes and antioxidant responses in different tissues of brown bullheads from relatively polluted and non-polluted systems. | brown bullheads (ameiurus nebulosus) were collected from the st. lawrence river and compared in their detoxication capacities to bullheads from a relatively non-polluted aquatic system, lac la pêche. the content of polychlorinated biphenyls (pcbs) in white muscle was significantly higher (22-fold) in bullheads from the st. lawrence river compared with those from lac la pêche. activities of liver ethoxyresorufin o-deethylase (erod), a common phase i enzyme, were 2.8-fold higher in st. lawrence ri ... | 1996 | 8688001 |
| fish biliary polycyclic aromatic hydrocarbon metabolites estimated by fixed-wavelength fluorescence: comparison with hplc-fluorescent detection. | fixed wavelength fluorescence (ff) was compared to high-performance liquid chromatography with fluorescence detection (hplc-f) as an estimation of polycyclic aromatic hydrocarbon (pah) exposure to fish. two excitation/emission wavelength pairs were used to measure naphthalene- and benzo[a]pyrene (b[a]p)-type metabolites. early nonmetabolite fluorescent peaks were negligible in hplc chromatograms of bile of brown bullhead, white sucker, and common carp. linear regression analysis of ff and hplc-f ... | 1996 | 8930501 |
| in vitro infection of a cell line from ictalurus nebulosus with piscirickettsia salmonis. | piscirickettsia salmonis, the etiologic agent of salmonid rickettsial septicemia (srs), affects several species of salmonids. previous reports using the appearance of cytopathic effect (cpe) as the criterion for susceptibility, showed that piscirickettsia salmonis (atcc strain) can be grown in vitro in some cells lines derived from salmonid fish, but not in bb cells from brown bullhead (ictalurus nebulosus) and bf-2 cells from bluegill (lepomis macrochirus). in this study we describe growth of p ... | 1997 | 9008805 |
| roles of dt diaphorase in the genotoxicity of nitroaromatic compounds in human and fish cell lines. | the genotoxicity of nitroaromatic compounds was examined in two cultured cell lines, namely, a human hepatoma cell line, hepg2, and a brown bullhead fibroblast cell line, bb. furthermore, the role of the quinone-reducing enzyme dt diaphorase [nad(p)h:(quinone acceptor) oxidoreductase] was examined with respect to its influence on the genotoxic effects of model nitroaromatic pollutants. the nitroreductive characteristics of these two cell lines were examined using an acetylated cytochrome c reduc ... | 1997 | 9310146 |
| comparison of alkaline single cell gel (comet) and peripheral blood micronucleus assays in detecting dna damage caused by direct and indirect acting mutagens. | the alkaline single cell gel (scg) or 'comet' and peripheral blood micronucleus (pbmn) assay have been used to compare the effects of the direct acting mutagens, methyl methanesulfonate (mms) and n-nitroso-n-methylurea (nmu), and the indirect acting mutagens, benzo[a]pyrene (bap), cyclophosphamide (cp) 9, 10-dimethyl-1,2-benzanthracene (dmba), and mitomycin c (mmc) in an inbred house mouse (mus domesticus) strain. the alkaline scg assay was able to detect dna damage from direct acting mutagens. ... | 1997 | 9403028 |
| fathead minnow (pimephales promelas) vitellogenin: purification, characterization and quantitative immunoassay for the detection of estrogenic compounds. | the egg yolk precursor protein, vitellogenin (vtg), was purified from blood plasma of 17beta-estradiol (e2)-treated male fathead minnows (pimephales promnelas) by anion-exchange chromatography on deae-agarose. a rabbit antiserum was raised against their blood plasma and then adsorbed with plasma from untreated (control) males to render the antiserum specific to vtg. the adsorbed antiserum was used to detect fathead minnow vtg (fvtg) in western and dot blotting experiments and in an enzyme-linked ... | 1999 | 10442820 |
| mitogenic activity of fetal bovine serum, fish fry extract, insulin-like growth factor-i, and fibroblast growth factor on brown bullhead catfish cells--bb line. | bioassays were performed to assess the effects of different levels of growth medium supplementation with fetal bovine serum (fbs), fish fry extract (fe), combinations of fbs and fe, and addition of insulin-like growth factor i (igf-i) and fibroblast growth factor (fgf) on the proliferation of brown bullhead catfish cells (bb line). treatments (n = 4) were: 2.5, 5, 10, and 15.0% fbs or fe and 5/2.5, 5/5, 10/2.5, and 10/5 of a fbs/fe combination as supplement to the growth medium, or the addition ... | 1999 | 10765463 |
| brown bullhead (ameiurus nebulosus) skin carcinogenesis. | alternative models using fish species have been tested in liver toxicity and carcinogenesis bioassays. similar models have not been developed for skin. the brown bullhead (ameiurus nebulosus) has shown potential as a model for skin carcinogenesis studies due to its sensitivity to environmental chemical pollutants. the present study is an initial morphologic and biochemical characterization of the normal and neoplastic brown bullhead skin to assess its suitability as a model of skin carcinogenesi ... | 2000 | 10930121 |
| characterization of three corticotropin-releasing factor receptors in catfish: a novel third receptor is predominantly expressed in pituitary and urophysis. | the present study reports the isolation of three complementary dna (cdna) clones encoding distinct subtypes of crf receptors from the diploid catfish (cf) species, ameiurus nebulosus. the first clone encodes a 446-amino acid protein (cfcrf-r1) that is highly homologous to mouse (m) crf-r1 (93% identical). the cfcrf-r1 messenger rna is highly expressed in the brain, and its distribution pattern correlates well with that of mammalian crf-r1, except for weak expression in the pituitary. when transi ... | 2001 | 11145609 |
| no detectable dna excision repair in uv-exposed hepatocytes from two catfish species. | dna repair is a critical process in protecting cellular genetic information from mutation. nucleotide excision repair (ner) is a mechanism by which cells correct dna damage caused by agents that form bulky covalent adducts and uv photoproducts such as thymine dimers and 6-4 photoproduct. ner, sometimes called dark repair, is generally accepted as being low in fish compared to mammals. this study was designed to quantitate ner in two related catfish species that have known differential sensitivit ... | 2001 | 11255108 |
| amphibian glucagon family peptides: potent metabolic regulators in fish hepatocytes. | peptides analogous to glucagon-like peptide-1 (glp-1) have been isolated from amphibian pancreas and intestine, and their amino acid sequences and cdna structures elucidated. just like their mammalian counterpart, these peptides are potent insulinotropins in mammalian pancreatic cells. we show here that these peptides also exert strong glycogenolytic actions when applied to dispersed fish hepatocytes. we compared the potencies of three synthetic glp-1s from xenopus laevis and two native glp-1s f ... | 2001 | 11384772 |
| metabolism of chrysene by brown bullhead liver microsomes. | we have investigated the regio- and stereoselective metabolism of chrysene, a four-ring symmetrical carcinogenic polycyclic aromatic hydrocarbon (pah), by the liver microsomes of brown bullhead (ameriurus nebulosus), a bottom-dwelling fish species. the liver microsomes from untreated and 3-methylcholanthrene (3-mc)-treated brown bullheads metabolized chrysene at the rate of 30.1 and 82.2 pmol/mg protein/min, respectively. benzo-ring diols (1,2-diol and 3,4-diol) were the major chrysene metabolit ... | 2003 | 12520076 |
| apparent diffusion limitations on branchial co2 transfer are revealed by severe experimental anaemia in brown bullhead (ameiurus nebulosus). | in this study, the rapid (within 2 h) effects of acute anaemia on blood gas and acid-base status, as well as cardiorespiratory variables, were examined in brown bullhead (ameiurus nebulosus). anaemia was induced by blood withdrawal coupled to volume replacement with saline. lowering haematocrit from the control value of 23.5+/-1.0% (mean+/-s.e.m.; n=37) to 5.9+/-0.3% (n=37) resulted in a significant increase (by 2.63+/-0.51 torr; n=7) in arterial co(2) tension (paco(2)) over the subsequent 2-h p ... | 2003 | 12727553 |
| evidence of aryl hydrocarbon receptor ligands in presque isle bay of lake erie. | the purpose of the present studies was to use a biomarker approach to examine xenobiotic exposure of brown bullhead in presque isle bay, lake erie (usa). in particular, the presence of compounds that act through the aryl hydrocarbon receptor (ahr) was of interest due to its central role in gene regulation and carcinogenesis of dioxins and certain polycyclic aromatic hydrocarbons (pahs). initial screening of presque isle bay sediment samples by gene expression microarray in mouse hepatocytes reve ... | 2003 | 12842597 |
| factors affecting food chain transfer of mercury in the vicinity of the nyanza site, sudbury river, massachusetts. | the influence of the nyanza chemical waste dump superfund site on the sudbury river, massachusetts, was assessed by analysis of sediment, fish prey organisms, and predator fish from four locations in the river system. whitehall reservoir is an impoundment upstream of the site, and reservoir #2 is an impoundment downstream of the site. cedar street is a flowing reach upstream of the site, and sherman bridge is a flowing reach downstream of the site. collections of material for analysis were made ... | 2003 | 12858964 |
| metabolism of phenanthrene by brown bullhead liver microsomes. | we have investigated the regio- and stereoselective metabolism of phenanthrene by the liver microsomes of brown bullhead (ameriurus nebulosus), a bottom dwelling fish species. the liver microsomes from untreated and 3-methylcholanthrene (3-mc)-treated brown bullheads metabolized phenanthrene at a rate of 14.1 and 20.7 pmol/mg protein/min, respectively, indicating that the hydrocarbon is a rather poor substrate for bullhead liver microsomes contrary to what has been reported for rat liver microso ... | 2003 | 12878411 |
| glutathione s-transferase expression in pollution-associated hepatic lesions of brown bullheads (ameiurus nebulosus) from the cuyahoga river, cleveland, ohio. | in rodents, overexpression of glutathione s-transferase pi is a characteristic feature of foci of cellular alteration (fca) and neoplastic liver lesions induced by genotoxic chemicals. alterations of glutathione s-transferase (gst) expression in hepatic lesions have also been reported in fish exposed to environmental carcinogens, and cellular gst expression may be an important determinant of growth and progression of chemical-associated liver tumors in certain fish species. in the present study, ... | 2004 | 15071171 |
| endocrine disruption and altered gonadal development in white perch (morone americana) from the lower great lakes region. | high prevalences of gonadal intersex have been observed in wild fish populations in areas affected by domestic and industrial effluents. for this study, fish were collected in 1998 from the cootes paradise region of hamilton harbour in western lake ontario, canada, to determine whether gonadal abnormalities, including intersex, were present in young of the year (yoy) fish. no gonadal abnormalities were observed in goldfish (carassius auratus), common carp (cyprinus carpio), gizzard shad (dorosom ... | 2004 | 15175179 |
| procrh in the teleost ameiurus nebulosus: gene cloning and role in lps-induced stress response. | the procorticotrophin-releasing hormone (procrh) gene from the teleost ameiurus nebulosus was cloned by direct and inverse pcr-based technologies and characterized. the structure of the procrh gene shows the presence of four exons and three introns giving a total length of 1416bp. sequence similarity with the corresponding procrh coding sequences in tilapia mossambica and homo sapiens is 97.7 and 78%, respectively. western blot experiments performed with an anti-human crh (1-41) antibody reveale ... | 2004 | 15265538 |
| diagnostic criteria for proliferative hepatic lesions in brown bullhead ameiurus nebulosus. | brown bullhead ameiurus nebulosus is used as indicator species for contaminant effects at areas of concern (aoc) in the great lakes and other areas. one of the beneficial use impairments at numerous aoc is 'fish tumors and other deformities'. an impairment occurs when the prevalence of fish tumors and other deformities exceeds those at unimpacted or control sites or when survey data confirm the presence of neoplastic or preneoplastic liver lesions in bullhead or white sucker catostomus commerson ... | 2006 | 17067070 |
| establishment, characterization, and viral susceptibility of two cell lines derived from goldfish carassius auratus muscle and swim bladder. | goldfish carassius auratus are common aquarium fish and have a significant economic and research value, having considerable worth to fisheries as a baitfish and the ability to adapt to a range of habitats. two cell lines were established from goldfish muscle and swim bladder tissue, in order to create a biological monitoring tool for viral diseases. cell lines were optimally maintained at 30 degrees c in leibovitz-15 medium supplemented with 20% fetal bovine serum. propagation of goldfish cells ... | 2007 | 17972754 |
| morphology and 18s rdna of henneguya gurlei (myxosporea) from ameiurus nebulosus (siluriformes) in north carolina. | henneguya gurlei was isolated from ameiurus nebulosus captured in north carolina and redescribed using critical morphological features and 18s small-subunit ribosomal rna (ssu rdna) gene sequence. plasmodia are white, spherical, or subspherical, occur in clusters, measure up to 1.8 mm in length, and are located on the dorsal, pectoral, and anal fins. histologically, plasmodia are located in the dermis and subdermally, and the larger cysts disrupt the melanocyte pigment layer. the spore body is l ... | 2008 | 18372621 |
| aroclor 1248 exposure leads to immunomodulation, decreased disease resistance and endocrine disruption in the brown bullhead, ameiurus nebulosus. | the brown bullhead ameiurus nebulosus is a species of the family ictaluridae commonly used as a sentinel of environmental contamination. while these fish have been utilized for this purpose in areas contaminated with polychlorinated biphenyls (pcbs), few controlled, laboratory-based studies have been designed to document the effects of pcb mixtures in this species. here, brown bullhead were exposed to the pcb mixture, aroclor 1248, via intraperitoneal injection and the effects on immune function ... | 2009 | 19406486 |
| ultrastructure of the early gravid uterus of corallobothrium fimbriatum (cestoda: proteocephalidea). | the ultrastructure of the uterine epithelium and underlying extracellular matrices has been studied in several cyclophyllideans, but relatively little has been published on uterine fine structure in other cestode orders. to develop comparative data, we initiated a study focusing on a common and widespread genus of the order proteocephalidea. adult specimens of corallobothrium fimbriatum were removed from the small intestine of brown bullhead catfish, ameiurus nebulosus, from the st. lawrence riv ... | 2009 | 19468751 |
| transplantable epitheliomas of the lip and mouth of catfish : i. pathology. transplantation to anterior chamber of eye and into cornea. | the catfish (ameiurus nebulosus) taken from streams near philadelphia, is commonly afflicted with an epithelial tumor bearing some resemblance to epithelioma of the lip in man. this neoplasm usually occurs as solitary or multiple, large, red, fleshy masses upon the lips or dental plates, and by reason of its size, may prevent closure of the mouth. the tumor is comprised of epithelial cells, often in papillary arrangement, supported by a delicate vascularized connective tissue stroma. the larger ... | 1941 | 19871143 |
| ultrastructure of the early embryonic stages of corallobothrium fimbriatum (cestoda: proteocephalidea). | cellular details of early embryogenesis have been studied extensively among cyclophyllidean cestodes, but have been reported for only 2 species of the order proteocephalidea, both belonging to the genus proteocephalus. thus, we performed a detailed ultrastructural analysis of early embryos of a second species, corallobothrium fimbriatum, including early events in the formation of the embryonic envelopes. adult worms were collected from the small intestine of brown bullhead catfish, ameiurus nebu ... | 2010 | 20465318 |
| temperature acclimation in the nervous system of the brown bullhead (ictalurus nebulosus). | | 2015 | 4400040 |
| [pao2 changes during rapid pwo2 changes in 2 teleosts with different biotopes: salmo gairdnerii and ictalurus melas (author's transl)]. | pao2 and ventilation measurements in salmo gairdnerii and ictalurus melas (brown bullhead) were performed during progressive or step changes of pwo2. 10 in salmo, out of stress conditions at 15 degrees c, the pao2--pwo2 curve shows a plateau related to the pwo2 values above adaptation (155 torr). the pao2 value corresponds to the o2 maximum saturation of the arterial blood. in contrast, in salmo under stress conditions, pao2 increases linearly with pwo2. during a hypoxic step change, pao2 displa ... | 2003 | 1223262 |
| mechanisms of ion and acid-base regulation at the gills of freshwater fish. | this review examines the branchial mechanisms utilized by freshwater fish to regulate internal acid-base status and presents a model to explain the underlying basis of the compensatory processes. rainbow trout, oncorhynchus mykiss, and brown bullhead, ictalurus nebulosus, were examined under a variety of experimental treatments which induced respiratory and metabolic acid-base disturbances. acid-base regulation was achieved by appropriate adjustments of na+ and cl- net fluxes across the gills wh ... | 2006 | 1500882 |
| species-specific markers provide molecular genetic evidence for natural introgression of bullhead catfishes in hungary. | since three bullhead catfish species were introduced to europe in the late 19th century, they have spread to most european countries. in hungary, the brown bullhead (ameiurus nebulosus) was more widespread in the 1970s-1980s, but the black bullhead (ameiurus melas) has gradually supplanted since their second introduction in 1980. the introgressive hybridization of the two species has been presumed based on morphological examinations, but it has not previously been supported by genetic evidence. ... | 2017 | 28265489 |
| halogenated phenolic compounds in wild fish from canadian areas of concern. | concentrations of halogenated phenolic compounds were measured in the plasma of brown bullhead (ameiurus nebulosus) from 4 canadian areas of concern (aocs), to assess exposure to suspected thyroid-disrupting chemicals. hydroxylated polychlorinated biphenyls (oh-pcbs) were detected in every sample collected in 3 of the aocs; the detection frequency was lower in samples from the detroit river aoc. the oh-pcbs most frequently detected were pentachloro, hexachloro, and heptachloro congeners, which a ... | 2017 | 28256742 |
| polybrominated diphenyl ethers (pbdes) in great lakes fish: levels, patterns, trends and implications for human exposure. | levels of polybrominated diphenyl ethers (pbdes) were measured in edible portions of great lakes fish, with the goal of examining patterns/trends and evaluating implications for human exposure. a total of 470 fillets of 18 fish species collected from various parts of the canadian waters of the great lakes between 2006 and 2013 were analyzed for 17 (expanded to 33 in 2009) pbdes. for a limited number of species, fillet to whole body and fillet to eggs pbdes were compared to examine pattern and co ... | 2017 | 27865120 |
| whole genome sequencing and phylogenetic characterization of brown bullhead (ameiurus nebulosus) origin ranavirus strains from independent disease outbreaks. | ranaviruses are emerging pathogens associated with high mortality diseases in fish, amphibians and reptiles. here we describe the whole genome sequence of two ranavirus isolates from brown bullhead (ameiurus nebulosus) specimens collected in 2012 at two different locations in hungary during independent mass mortality events. the two hungarian isolates were highly similar to each other at the genome sequence level (99.9% nucleotide identity) and to a european sheatfish (silurus glanis) origin ran ... | 2016 | 27717748 |
| histopathology of brown bullhead (ameiurus nebulosus), smallmouth bass (micropterus dolomieu), and yellow perch (perca flavescens) in relation to polychlorinated biphenyl (pcb) contamination in the hudson river. | from the 1940s through 1977, at least 590,000kg of polychlorinated biphenyls (pcbs) were released into the hudson river from general electric manufacturing plants located in hudson falls and fort edward, new york. in 1984, the u.s. environmental protection agency designated a nearly 322km reach as the hudson river pcbs superfund site. here we describe a fish health assessment study, part of a natural resource damage assessment, that evaluated the prevalence of toxicopathic lesions in adult brown ... | 2017 | 27751691 |
| general and histological indicators of health in wild fishes from a biological mercury hotspot in northeastern north america. | kejimkujik national park and national historic site, nova scotia, canada, is considered a biological mercury (hg) hotspot because the tissues of yellow perch (perca flavescens) and common loons (gavia immer) inhabiting the lakes frequently exceed so-called safe levels of hg. in the present study, the relationships between hg and overall health of males and females of 3 forage fish species (brown bullhead ameirus nebulosus, banded killifish fundulus diaphanus, and golden shiner notemigonus crysol ... | 2017 | 27595668 |
| transcriptome discovery in non-model wild fish species for the development of quantitative transcript abundance assays. | environmental studies increasingly identify the presence of both contaminants of emerging concern (cecs) and legacy contaminants in aquatic environments; however, the biological effects of these compounds on resident fishes remain largely unknown. high throughput methodologies were employed to establish partial transcriptomes for three wild-caught, non-model fish species; smallmouth bass (micropterus dolomieu), white sucker (catostomus commersonii) and brown bullhead (ameiurus nebulosus). sequen ... | 2016 | 27497300 |
| stress in the neighborhood: tissue glucocorticoids relative to stream quality for five species of fish. | anthropogenic alterations to terrestrial habitat (e.g., urbanization, deforestation, agriculture) can have a variety of negative effects on watercourses that flow through disturbed landscapes. currently, the relationship between stream habitat quality and fish condition remains poorly understood. the use of physiological metrics such as glucocorticoids (gcs) provides a useful tool for quantifying these effects by relating the health of resident fishes to stream quality. to date, however, most st ... | 2016 | 26780133 |
| monitoring exposure of brown bullheads and benthic macroinvertebrates to sediment contaminants in the ashtabula river before, during, and after remediation. | in 2007, approximately 420,500 cubic meters of contaminated sediment were removed from the ashtabula river by dredging. the primary objective of the present study was to monitor contaminant exposure in fish and macroinvertebrates before, during, and after dredging. this was done by measuring tissue concentrations of polychlorinated biphenyl (pcb) and polycyclic aromatic hydrocarbon (pah) in brown bullhead catfish (ameriurus nebulosa) and in benthic macroinvertebrates, assessing changes in dna da ... | 2015 | 25565098 |
| benzo(a)pyrene exposure causes adaptive changes in p53 and cyp1a gene expression in brown bullhead (ameiurus nebulosus). | the brown bullhead (ameiurus nebulosus) is able to survive and reproduce in high levels of environmentally contaminated areas of the great lakes. the purpose of this study was to establish whether there are adaptive genetic/molecular changes occurring in these fish that allow for their survival. expression of a cell cycle regulator, p53 and the toxin metabolizing protein, cyp1a were measured in liver tissue from bullhead caught from either clean or contaminated areas of lake erie and surrounding ... | 2014 | 25259779 |
| temporal and spatial patterns in tumour prevalence in brown bullhead ameiurus nebulosus (lesueur) in the tidal potomac river watershed (usa). | for two decades, fish tumour surveys have been used to monitor habitat quality in the chesapeake bay (usa) watershed. tributaries with sediments contaminated with polynuclear aromatic hydrocarbons (pahs), known to cause liver neoplasia, were frequently targeted. here, we compare surveys in brown bullhead ameiurus nebulosus conducted in 2009-2011 in the tidal potomac river watershed (including the anacostia river) with previous surveys. using logistic regression, we identified length and sex as c ... | 2014 | 24974857 |
| wild fish from the bay of quinte area of concern contain elevated tissue concentrations of pcbs and exhibit evidence of endocrine-related health effects. | the bay of quinte (boq) is an area of concern listed under the great lakes water quality agreement. the presence of dioxins and dioxin-like pcbs in fish in the boq aoc has led to restrictions on fish consumption by humans, which is a beneficial use impairment. adult yellow perch (perca flavescens) and brown bullhead (ameiurus nebulosus) were sampled from trenton, belleville, and deseronto (reference site) in the boq. a suite of hormone assays and various measures of exposure and/or sublethal hea ... | 2014 | 24576942 |
| associations between female reproductive traits and polychlorinated biphenyl sediment concentrations in wild populations of brown bullhead (ameiurus nebulosus). | aquatic contaminants, specifically polychlorinated biphenyls (pcbs), a class of persistent organic contaminants, have been associated with sublethal effects on reproduction in fishes. female brown bullhead (ameiurus nebulosus) were used to assess variation in reproductive traits across eight populations differing in sediment sum pcb concentrations in the lower great lakes region. differences in maternal carotenoid allocation patterns among these populations were also examined. no significant ass ... | 2013 | 23887386 |
| an investigation of enhanced mercury bioaccumulation in fish from offshore feeding. | we investigated the dietary pathways of mercury transfer in the food web of morency lake (canada) to determine the influence of carbon source and habitat use on mercury bioaccumulation in fish. whole-body concentrations of methylmercury (mehg) were significantly different in four fish species (white sucker, brown bullhead, pumpkinseed and smallmouth bass) and increased with both trophic position and greater feeding on offshore (versus littoral) carbon. an examination of fish gut contents and the ... | 2013 | 23748886 |
| polybrominated diphenyl ethers and their methoxylated and hydroxylated analogs in brown bullhead (ameiurus nebulosus) plasma from lake ontario. | polybrominated diphenyl ethers (pbdes), methoxylated pbdes (meo-pbdes) and hydroxylated pbdes (oh-pbdes) were detected and quantified in brown bullhead (ameiurus nebulosus) from lake ontario. samples were collected in 2006 from three different locations near the city of toronto: frenchman's bay, toronto island, and tommy thompson park. a total of 117 plasma samples were pooled into 19 samples, separating males and females by site of capture. pooled samples were analyzed for 36 pbdes, 20 meo-pbde ... | 2013 | 23121987 |
| polychlorinated biphenyls and their hydroxylated metabolites in wild fish from wheatley harbour area of concern, ontario, canada. | whole-body polychlorinated biphenyls (σpcbs) and plasma hydroxylated pcbs (oh-pcbs) concentrations were determined in brown bullhead (ameiurus nebulosus) from wheatley harbour, ontario, canada. elevated σpcbs in wheatley harbour are suspected to have originated from industrial waste disposal and/or discharges from nearby fish processing through discarding of fish remains. mean σpcb concentrations in brown bullhead from wheatley harbour were approximately 250 ng/g wet weight compared with approxi ... | 2012 | 23027467 |
| assessment of the health status of wild fish from the wheatley harbour area of concern, ontario, canada. | the overall health and endocrine function of wild brown bullhead (ameiurus nebulosus) and goldfish (carassius auratus) from the wheatley harbour area of concern (lake erie, ontario, canada) was assessed using a suite of physiological and biochemical endpoints. smaller gonads were detected in female brown bullhead and goldfish from wheatley harbour compared with hillman marsh (ontario, canada) reference fish. female brown bullhead exhibited decreased in vitro synthesis of 17β-estradiol. female go ... | 2012 | 23027428 |
| rotenone formulation fate in lake davis following the 2007 treatment. | in september 2007, lake davis (near portola, california) was treated by the california department of fish and game with cft legumine, a rotenone formulation, to eradicate the invasive northern pike (esox lucius). the objective of this report is to describe the fate of the five major formulation constituents-rotenone, rotenolone, methyl pyrrolidone (mp), diethylene glycol monethyl ether (degee), and fennedefo 99-in water, sediment, and brown bullhead catfish (ameiurus nebulosus; a rotenone-resist ... | 2012 | 22407799 |
| acclimation of life-history traits to experimental changes in environmental contaminant concentrations in brown bullhead (ameiurus nebulosus). | one adaptive mechanism aquatic populations use to facilitate tolerance to environmental contaminants is acclimation. polychlorinated biphenyls (pcbs) are a globally ubiquitous class of persistent organic contaminants that have been linked to reproductive impairments in fish. the authors used female brown bullhead (ameiurus nebulosus) to test whether acclimation of reproductive life-history traits occurs in response to changes in sum pcb exposure. they compared egg diameter, gonadosomatic index ( ... | 2012 | 22278858 |
| tumor prevalence and biomarkers of genotoxicity in brown bullhead (ameiurus nebulosus) in chesapeake bay tributaries. | we surveyed four chesapeake bay tributaries for skin and liver tumors in brown bullhead (ameiurus nebulosus). we focused on the south river, where the highest skin tumor prevalence (53%) in the bay watershed had been reported. the objectives were to 1) compare tumor prevalence with nearby rivers (severn and rhode) and a more remote river (choptank); 2) investigate associations between tumor prevalence and polynuclear aromatic hydrocarbons (pahs) and alkylating agents; and 3) statistically analyz ... | 2011 | 21995877 |
| histologic, immunologic and endocrine biomarkers indicate contaminant effects in fishes of the ashtabula river. | the use of fish as sentinels of aquatic ecosystem health is a biologically relevant approach to environmental monitoring and assessment. we examined the health of the ashtabula river using histologic, immunologic, and endocrine biomarkers in brown bullhead (bb; ameiurus nebulosus) and largemouth bass (micropterus salmoides) and compared fish collected from a reference site (conneaut creek). seasonal analysis was necessary to distinguish differences in fish between the two rivers. overall bb from ... | 2012 | 21882039 |
| long-term trends in liver neoplasms in brown bullhead in the buffalo river, new york, usa. | the buffalo river area of concern (aoc) was assigned an impaired status for the fish tumors and other deformities beneficial use impairment category by the new york state department of environmental protection in 1989. this was initially based on an inadequately documented brown bullhead (ameiurus nebulosus) feeding study using river sediment extracts. the presence of liver tumors was subsequently supported by reports of a 19 to 27% prevalence in wild brown bullhead between 1983 and 1988 and a 4 ... | 2010 | 20821628 |
| pollution-induced behavioural effects in the brown bullhead (ameiurus nebulosus). | aquatic ecosystems are major sinks for pollutants which can have adverse effects on biodiversity. thus, it is important to understand the nature of pollution-induced change in aquatic ecosystems. we show that brown bullheads (ameiurus nebulosus) may have evolved in response to chronic pollution exposure. we collected adults from the detroit river (polluted site) and belle river (control site). both adults and common-garden raised juveniles were tested for aggression, locomotion, and escape respo ... | 2010 | 20640507 |
| tumors in brown bullheads in the chesapeake bay watershed: analysis of survey data from 1992 through 2006. | liver and skin tumor prevalences in brown bullheads ameiurus nebulosus have been used in the north american great lakes to designate highly contaminated areas of concern and monitor their recovery. here we interpret the results of six surveys conducted in the chesapeake bay watershed between 1992 and 2006, with data for 647 fish. the objective has been to develop an adequate database to critically evaluate the use of tumor prevalence as a habitat quality indicator within the watershed. surveys f ... | 2009 | 19873828 |
| fish communities in coastal freshwater ecosystems: the role of the physical and chemical setting. | we explored how embayment watershed inputs, morphometry, and hydrology influence fish community structure among eight embayments located along the southeastern shoreline of lake ontario, new york, usa. embayments differed in surface area and depth, varied in their connections to lake ontario and their watersheds, and drained watersheds representing a gradient of agricultural to forested land use. | 2008 | 19114002 |
| comparative evaluation of the cytotoxicity sensitivity of six fish cell lines to four heavy metals in vitro. | to establish the potential use of cell cultures as a simple and sensitive biological tool to detect environmental pollutants, six cell lines established from several fish species including gcf (grass carp fins), cik (ctenopharyngodon idellus kidney), epc (epithelioma papulosum cyprini), cco (channel catfish ovary), bb (brown bullhead caudal trunk) and fhm (fathead minnow muscle) were tested and compared for their cytotoxic sensitivity to four heavy metals: cadmium (cd), chromium (cr), zinc (zn), ... | 2008 | 17931828 |
| dna damage and external lesions in brown bullheads (ameiurus nebulosus) from contaminated habitats. | the comet assay was used to compare levels of dna damage in brown bullheads (ameiurus nebulosus) collected from three known contaminated locations, the cuyahoga river (oh, u.s.a.), ashtabula river (oh, u.s.a.; both tributaries to lake erie, usa), and ashumet pond (cape cod, ma, u.s.a.), with brown bullheads collected from three paired reference sites, old woman creek (oh, u.s.a.), conneaut river (oh, u.s.a.; both tributaries to lake erie), and great herring pond (mainland ma, u.s.a.), respective ... | 2006 | 17089728 |
| cumulative impacts assessment along a large river, using brown bullhead catfish (ameiurus nebulosus) populations. | the effects of point-source and diffuse discharges on resident populations of brown bullhead catfish (ameiurus nebulosus (lesueur, 1819)) in the waikato river (new zealand) were assessed at sites both upstream and downstream of point-source discharges. at each site, the population parameters, relative abundance, age structure, and individual indices, such as condition factor, organ (gonad, liver, and spleen) to somatic weight ratios, and number and size of follicles per female, were assessed. ph ... | 2006 | 16833150 |
| tumor prevalence and biomarkers of exposure and response in brown bullhead (ameiurus nebulosus) from the anacostia river, washington, dc and tuckahoe river, maryland, usa. | we evaluated liver and skin tumor prevalence and biomarkers of exposure and response in brown bullhead (ameiurus nebulosus) from three locations in the anacostia river (washington, dc, usa), a chesapeake bay region of concern. the tuckahoe river (maryland, usa) served as a reference. each river was sampled in fall 2000 and spring 2001. in the anacostia, prevalence of liver tumors was 50 to 68%, and prevalence of skin tumors was 13 to 23% in large (> or = 260 mm, age > or = 3 years) bullheads. li ... | 2004 | 15285357 |
| zebra mussels affect benthic predator foraging success and habitat choice on soft sediments. | the introduction of zebra mussels ( dreissena spp.) to north america has resulted in dramatic changes to the complexity of benthic habitats. changes in habitat complexity may have profound effects on predator-prey interactions in aquatic communities. increased habitat complexity may affect prey and predator dynamics by reducing encounter rates and foraging success. zebra mussels form thick contiguous colonies on both hard and soft substrates. while the colonization of substrata by zebra mussels ... | 2004 | 15278426 |
| tumor prevalence and biomarkers of exposure in brown bullhead (ameiurus nebulosus) from back river, furnace creek, and tuckahoe river, maryland. | brown bullheads (ameiurus nebulosus) were collected from 2 locations near baltimore, maryland, back river and furnace creek, and 1 (reference) location, tuckahoe river, to compare the prevalence of tumors (liver and skin) and visible skin lesions (fin erosion and abnormal barbels). cytochrome p450 activity measured as ethoxyresorufin-o-deethylase, biliary pah-like fluorescent metabolites, and fillet contaminant concentrations were determined as indicators of exposure in a randomly selected subse ... | 2004 | 15253047 |
| sensitive genetic biomarkers for determining apoptosis in the brown bullhead (ameiurus nebulosus). | biomarkers are necessary for monitoring environmentally induced alterations at the molecular level in order to assess the impact of xenobiotic compounds on organism health. apoptosis is a highly regulated cellular process that controls programmed cell death and is involved in tumor formation. apoptosis thus may provide the basis for developing biomarkers for use in the field of ecotoxicology to monitor non-lethal levels of xenobiotic induced cellular stress and toxicity. this study shows that a ... | 2004 | 15033523 |
| tissue engineering of fish skin: behavior of fish cells on poly(ethylene glycol terephthalate)/poly(butylene terephthalate) copolymers in relation to the composition of the polymer substrate as an initial step in constructing a robotic/living tissue hybrid. | this study presents the development of a biosynthetic fish skin to be used on aquatic robots that can emulate fish. smoothness of the external surface is desired in improving high propulsive efficiency and maneuvering agility of autonomous underwater vehicles such as the robotuna (triantafyllou, m., and triantafyllou, g. sci. am. 272, 64, 1995). an initial step was to determine the seeding density and select a polymer for the scaffolds. the attachment and proliferation of chinook salmon embryo ( ... | 2006 | 15009926 |
| cell volume affects glycogen phosphorylase activity in fish hepatocytes. | the activity of glycogen phosphorylase (gpase) in the active a-form (gpase a) is dependent on the hydration state of hepatocytes. we establish that gpase a catalysis in catfish (ameiurus nebulosus) hepatocytes is a function of medium osmolarity and that a linear relationship exists between gpase a activity and osmolarity between 254 mosmol l(-1) and 478 mosmol l(-1). exposure of isolated hepatocytes to hyperosmotic media increases enzyme activity up to 7-fold, indicative of covalent phosphorylat ... | 2003 | 12898165 |
| high plasma buffering and the absence of a red blood cell beta-nhe response in brown bullhead (ameiurus nebulosus). | the high non-bicarbonate buffer capacity of brown bullhead (ameiurus nebulosus) plasma was postulated to function as an alternative mechanism for the protection of red blood cell (rbc) intracellular ph (phi) in the absence or attenuation of a rbc adrenergic response. the requirement for protecting rbc phi arises from the presence of a root effect haemoglobin in bullhead. in support of this hypothesis, bullhead rbcs incubated in vitro with isoproterenol (10(-8)-10(-5) mol l(-1)) or forskolin (10( ... | 2002 | 12208310 |
| novel role for prostaglandin e2 in fish hepatocytes: regulation of glucose metabolism. | prostaglandin e(2) (pge(2)) potently activated glycogenolysis and gluconeogenesis in isolated rockfish (sebastes caurinus) hepatocytes. the average degree of activation for glycogenolysis was 6.4+/-0.67-fold (mean+/-s.e.m.; n=37), and could be as much as 19-fold. analysis of dose-concentration relationships between glycogenolytic actions and pge(2) concentrations yielded an ec(50) around 120 nm in hepatocyte suspensions and 2 nm for hepatocytes immobilized on perifusion columns. for the activati ... | 2002 | 12098672 |
| dermal melanoma with schwannoma-like differentiation in a brown bullhead catfish (ictalurus nebulosus). | a dermal melanoma with histologic similarities to mammalian melanocytic schwannomas was diagnosed in a wild-caught brown bullhead catfish (ictalurus nebulosus). the diagnosis was based on cytologic, histopathologic, immunohistochemical, and ultrastructural findings. the neoplasm protruded from the cutaneous surface of the dorsal midline immediately caudal to the dorsal fin, was diffusely black and focally ulcerated, and was attached to the underlying skin by a broad-based stalk. cytologically, t ... | 2002 | 12033683 |
| an investigation of carbonic anhydrase activity in the gills and blood plasma of brown bullhead (ameiurus nebulosus), longnose skate (raja rhina), and spotted raffish (hydrolagus colliei). | separated plasma and whole blood non-bicarbonate buffering capacities, together with plasma and gill carbonic anhydrase activities and endogenous plasma carbonic anhydrase inhibitor activity were investigated in three species of fish: the brown bullhead (ameirus nebulosus), a teleost; the longnose skate (raja rhina), an elasmobranch; and the spotted ratfish (hydrolagus colliei), a chimaeran. the objective was to test the hypothesis that species possessing gill membrane-bound carbonic anhydrase a ... | 2002 | 11824406 |
| the buffering power of plasma in brown bullhead (ameiurus nebulosus). | brown bullhead (ameiurus nebulosus) blood plasma was found to exhibit an unusually high non-bicarbonate buffer capacity (beta) in relation to that of other teleost fish. in brown bullhead, the non-bicarbonate buffer capacity of plasma (beta(plasma)), at -5.72 +/- 0.34 mmol l(-1) ph unit(-1) (mean +/- s.e.m., n=30), constituted 37% of whole blood beta and was 2.5 times higher than beta(plasma) in rainbow trout (-2.33 +/- 0.42 mmol l(+/-1) ph unit(-1); n=7). the strong buffering power of bullhead ... | 2002 | 11818239 |
| chemical orientation of brown bullheads, ameiurus nebulosus, under different flow conditions. | the spatiotemporal information in chemical signals provides critical information for organisms during chemical orientation. information in chemical signals is influenced by the hydrodynamic conditions of the environment. hydrodynamically distinct environments will contain different types of information, which will influence how organisms orient. this study was designed to examine how the orientation behavior of the brown bullhead (ameiurus nebulosus) is influenced by flow regime. the experiment ... | 2001 | 11817083 |
| use of molecular markers to study the effects of environmental impacts on genetic diversity in brown bullhead (ameirus nebulosus) populations. | conservation biology needs sound biological information in order to maintain biological diversity in the face of the current rate of loss. an important component of the information needed is the level of genetic diversity within and between populations, especially for those species faced with exposure to environmental stressors. we applied multilocus dna profile analysis (highly variable number tandem repeats [hvntr] and randomly amplified polymorphic dna [rapd] techniques) and allozyme analysis ... | 2001 | 11699785 |
| decreased glutathione s-transferase expression and activity and altered sex steroids in lake apopka brown bullheads (ameiurus nebulosus). | a number of freshwater lakes and reclaimed agricultural sites in central florida have been the receiving waters for agrochemical and municipal runoff. one of these sites, lake apopka, is also a eutrophic system that has been the focus of several case studies reporting altered reproductive activity linked to bioaccumulation of persistent organochlorine chemicals in aquatic species. the present study was initiated to determine if brown bullheads (ameiurus nebulosus) from the north marsh of lake ap ... | 2001 | 11595311 |
| altered glutathione s-transferase catalytic activities in female brown bullheads from a contaminated central florida lake. | brown bullheads (ameriurus nebulosus) are a demersal freshwater species that can be found in a number of polluted ecosystems. the purpose of the present study was to determine the overall capacity for in vitro glutathione s-transferase (gst) detoxification by brown bullheads, and to see if bullhead gst catalysis was altered in bullheads from a polluted site. brown bullhead liver cytosolic gsts catalyzed the conjugation of 1-chloro-2,4-dinitrobenzene (cdnb) over a large range of substrate concent ... | 2013 | 11460726 |
| conservation of a glutathione s-transferase in marine and freshwater fish. | we have previously reported the isolation and cloning of glutathione s-transferase (gst) cdnas from two marine fish, english sole (pleuronectes vetulus) and starry flounder (platichthys stellatus), that exhibited > 95% identity to plaice (pleuronectes platessa) gst-a aquatic toxicol., 44, 171-182]. in the present study, we have used reverse transcription-polymerase chain reaction (rt-pcr) analysis to isolate a 471 nucleotide gst-like cdna from largemouth bass (micropterus salmoides) liver. seque ... | 2003 | 11460686 |
| induction of glutathione s-transferase activity and protein expression in brown bullhead (ameiurus nebulosus) liver by ethoxyquin. | the inducibility of hepatic cytosolic glutathione s-transferases (gsts) was examined in brown bullheads, a freshwater fish that is highly susceptible to hepatic neoplasia following exposure to carcinogen-contaminated sediments. juvenile bullheads were fed a semi-purified antioxidant-free diet supplemented with ethoxyquin (0.5% w/w dissolved in 3% corn oil), a prototypical rodent gst-inducing agent, twice daily for 14 days. control bullheads received the antioxidant-free diet supplemented with co ... | 2001 | 11399793 |
| tumor prevalence and biomarkers of exposure in brown bullheads (ameiurus nebulosus) from the tidal potomac river, usa, watershed. | associations between contaminant exposure and liver and skin tumor prevalence were evaluated in brown bullheads (ameiurus nebulosus) from the tidal potomac river, usa, watershed. thirty bullheads (> or = age 3) were collected from quantico embayment, near a superfund site that released organochlorine contaminants; neabsco creek, a tributary with petroleum inputs from runoff and marinas; and anacostia river (spring and fall), an urban tributary designated as a chesapeake bay region of concern, th ... | 2001 | 11392129 |
| comparative metabolism and excretion of benzo(a)pyrene in 2 species of ictalurid catfish. | differential susceptibility of polycyclic aromatic hydrocarbon (pah)-mediated liver cancer exists in two related species of ictalurid catfish. two hypotheses are addressed in this study to explain this difference. specifically, the relatively insensitive channel catfish 1) do not produce mutagenic pah metabolites, and/or 2) they more quickly eliminate pahs due to greater phase ii enzyme activities than the more sensitive brown bullhead. livers and bile were collected from each species 6, 24, 72, ... | 2000 | 11053542 |
| the behavioral detection of binary mixtures of amino acids and their individual components by catfish. | the question of whether a binary mixture of amino acids is detected by fish as a unique odor or whether the qualities of the individual components are retained within the mixture was investigated in channel (ictalurus punctatus) and brown bullhead (ameiurus nebulosus) catfish, species that are highly similar in their olfactory receptor and behavioral responses to amino acid odorants. catfish respond with greater appetitive food-searching (swimming) behavior to amino-acid-conditioned olfactory st ... | 2000 | 11023851 |
| in vitro response of the brown bullhead catfish (bb) and rainbow trout (rtg-2) cell lines to benzo[a]pyrene. | established cell lines from rainbow trout (rtg-2) and brown bullhead catfish (bb) were evaluated as bioindicators of benzo[a]pyrene (b[a]p) toxicity with 3-(4,5-dimethyltiazol-2-yl)-2,5-diphenyl tetrazolium bromide (mtt) reduction and neutral red (nr) uptake assays. cytochrome p450 1a1 (cyp1a1) enzymatic activity was also evaluated, and taken as a biological indicator of the b[a]p induction power by ethoxyresorufin o-deethylase (erod) and ethoxycoumarin o-deethylase (ecod) assays. the bb and rtg ... | 2000 | 10803541 |
| olfactory discrimination of amino acids in brown bullhead catfish. | olfactory discrimination of amino acids was investigated in brown bullhead catfish (ameiurus nebulosus). based on the magnitude of the observed food search activity of catfish conditioned to single amino acids, the tested compounds were classified as being detected by the catfish as equal to, similar to, or different from the conditioned stimulus. l-proline (l-pro)-conditioned brown bullhead catfish discriminated all amino acids from l-pro, but catfish conditioned to l-valine (l-val) and l-isole ... | 2000 | 10667990 |