| intestinal colonization potential of turbot (scophthalmus maximus)- and dab (limanda limanda)-associated bacteria with inhibitory effects against vibrio anguillarum. | of more than 400 bacteria isolated from turbot (scophthalmus maximus), 89 have previously been shown to inhibit the in vitro growth of the fish pathogen vibrio anguillarum. the aim of the present study was to investigate the potential of seven of these strains, as well as of intestinal isolates (four strains) from a closely related fish, dab (limanda limanda), for colonizing farmed turbot as a means of protecting the host from infection by v. anguillarum. in addition, the inhibitory effect of th ... | 1992 | 1610180 |
| environmental contaminants and biochemical responses in flatfish from the hvaler archipelago in norway. | the levels of several environmental contaminants, including selected polyaromatic hydrocarbons (pah), organochlorines (ddt/dde, hexachlorobenzene), 15 polychlorinated biphenyl (pcb) congeners, and polychlorinated dibenzofurans and dibenzo-p-dioxins, pcdf/pcdd), and heavy metals (cd, hg, pb, and as) were analyzed in muscle and liver of three different flatfish species (dab, limanda limanda; flounder, platichthys flesus; plaice, pleuronectes platessa) caught by gill netting at different sites in t ... | 1991 | 1759844 |
| levels of 17 alpha,20 alpha-dihydroxy-4-pregnen-3-one, 3 beta,17 alpha,20 alpha-trihydroxy-5 beta-pregnane, and other sex steroids, in blood plasma of male dab, limanda limanda (marine flatfish) injected with human chorionic gonadotrophin. | in a previous study, plasma sex steroid levels were measured in female dab (limanda limanda) induced to ovulate by injections of human chorionic gonadotrophin (hcg). in the present study, a similar experiment was carried out on male dabs. in common with female dabs, 17 alpha,20 alpha-dihydroxy-4-pregnen-3-one and 3 beta,17 alpha,20 alpha-trihydroxy-5 beta-pregnane showed the greatest response. their plasma levels increased, respectively, from 6 +/- 1.6 and 13 +/- 6.2 ng/ml to ca. 62 ng/ml within ... | 1991 | 1916213 |
| effects of steroids and human chorionic gonadotrophin on in vitro oocyte final maturation in two marine flatfish: the dab, limanda limanda, and the plaice, pleuronectes platessa. | this study investigated the role of gonadotrophin and steroids in the induction of in vitro oocyte maturation in dab (limanda limanda) and plaice (pleuronectes platessa), two marine flatfish (pleuronectiformes) in which the oocytes mature and ovulate in groups (or batches). clusters of folliculated oocytes were incubated for 3 days with a range of doses of either human chorionic gonadotrophin (hcg) or synthetic steroids with a wide range of structures. they were also incubated with extracts from ... | 1990 | 2307340 |
| plasma levels of ovarian steroids, including 17 alpha-20 alpha-dihydroxy-4-pregnen-3-one and 3 beta,17 alpha,20 alpha-trihydroxy-5 beta-pregnane, in female dabs (limanda limanda)--marine flatfish--induced to mature and ovulate with human chorionic gonadotrophin. | human chorionic gonadotrophin (hcg) effectively stimulated oocyte final maturation and ovulation in female dabs (limanda limanda) within 5 days of injection, and this was accompanied by significant changes in blood plasma steroid levels. the steroids which showed the greatest responses to the hcg injections were the ones previously found to be the major products of the ovaries in vitro: 17 alpha-20 alpha-dihydroxy-4-pregnen-3-one (17,20 alpha-p) and 3 beta,17 alpha,20 alpha-trihydroxy-5 beta-pre ... | 1990 | 2307341 |
| identification of, and development of radioimmunoassays for 17 alpha, 21-dihydroxy-4-pregnene-3,20-dione and 3 alpha,17 alpha, 21-trihydroxy-5 beta-pregnan-20-one in the ovaries of mature plaice (pleuronectes platessa). | ovaries from a female plaice (pleuronectes platessa) that had been injected with human chorionic gonadotrophin were incubated in vitro with 17 alpha-hydroxy[1,2,6,7-3h]progesterone. the major steroids produced by the ovaries were tentatively identified as 17 alpha,21-dihydroxy-4-pregnene-3,20di-one (11-deoxycortisol; 17,21-p), 17 alpha,21-dihydroxy-5 beta-pregnane-3,20-dione (3 alpha, 17,21-p-5 beta). a high proportion of these steroids was found in a conjugated form (sulphates or glucuronides). ... | 1990 | 2354767 |
| the life cycle of pygidiopsis ardeae køie, 1990 (digenea, heterophyidae). | ocellate cercariae, consistent with the description of cercaria angularis honer, 1961, developing in rediae in hydrobia ulvae (pennant) and hydrobia ventrosa (montagu) (prosobranchia, hydrobiidae) from denmark, were shown to be larvae of pygidiopsis ardeae køie, 1990 (heterophyidae, pygidiopsinae). euryhaline fishes such as the sticklebacks gasterosteus aculeatus linnaeus and pungitius pungitius (linnaeus), and the common goby pomatoschistus microps (krøyer) are second intermediate hosts in dani ... | 1990 | 2380862 |
| monitoring biological effects of contamination in marine fish along french coasts by measurement of ethoxyresorufin-o-deethylase activity. | the use of bioindicators to evaluate exposure to the biological effects of chemical pollutants in marine organisms constitutes a new tool in the monitoring field. the establishment of a north sea monitoring network in 1991, involving such international organizations as the north sea task force, the international council for the exploration of the sea, and the intergovernmental oceanography commission, led french researchers to develop an enzymatic biomarker to monitor biological effects within t ... | 1994 | 7533706 |
| isolation of a metal-binding protein from ovaries of dab (limanda limanda l.) distinct from metallothionein: effect of cadmium exposure. | metal-binding proteins were isolated from ovaries of dab (limanda limanda l.) caught in the north sea. separation of the heat-treated cytosolic fraction of dab ovaries by gel-filtration chromatography revealed the presence of a major zinc-binding protein with a low-molecular-weight of 10,000-12,000 da, similar to mammalian metallothionein (mt). the metal-binding protein was analyzed by anion-exchange chromatography and by reversedphase high-performance liquid chromatography. the metal-binding pr ... | 1996 | 8727522 |
| detection of specific and 'constitutive' antibody secreting cells in the gills, head kidney and peripheral blood leucocytes of dab (limanda limanda). | the relative immunological importance of the gills of fish was investigated in terms of antibody production by enumerating antibody secreting cells (asc) in the gills, head kidney and blood of dab (limanda limanda) using the elispot assay. the contribution of 'constitutive' asc in the gill appeared more substantial than that of elicited specific asc. the gills were found to contain a mean (+/- sd) of 4227 +/- 1029 'constitutive' asc/10(6) cells which was fewer than the head kidney which containe ... | 1997 | 9436279 |
| isolation of viral haemorrhagic septicaemia virus (vhsv) from wild marine fish species in the baltic sea, kattegat, skagerrak and the north sea. | in order to analyse the occurrence of viral haemorrhagic septicaemia virus (vhsv) in the marine environment surrounding denmark, fish tissue samples were collected on four cruises with the research vessel h/s dana in 1996 and 1997. the sampling comprised 923 samples totalling 7344 fish representing 29 different species. vhsv was isolated from 24 fish samples from the baltic sea, four samples from skagerrak and three samples from the north sea. the virus-positive host species included herring clu ... | 1999 | 10509720 |
| correlation between the level of the potential biomarker, heat-shock protein, and the occurrence of dna damage in the dab, limanda limanda: a field study in the north sea and the english channel. | in the present study, heat-shock protein of m(r) 70 kda (hsp70), a marker of cellular stress response, was validated as a potential biomarker under field conditions. the dab, limanda limanda (female, size > or = 25 cm, spawning maturity stage 2) was used as the indicator organism. the data on hsp level were correlated with the occurrence of dna damage, measured in the same specimens of l. limanda, to prove the usefulness of the method. the area under investigation was the north sea. four locatio ... | 2000 | 11285726 |
| potential value of the comet assay and dna adduct measurement in dab (limanda limanda) for assessment of in situ exposure to genotoxic compounds. | an in situ study of the relationship between marine contamination and genotoxic effects was performed on female dab (limanda limanda) collected from different sites in the eastern english channel (france) known to be contaminated by polycyclic aromatic hydrocarbons (pahs) and polychlorobiphenyls (pcbs). dna adducts in liver and dna strand breaks in blood cells were determined respectively by the nuclease p1-enhanced post-labelling technique and an alkaline version of the comet assay. the extent ... | 2003 | 12504752 |
| organohalogens and metals in marine fish and mussels and some relationships to biological variables at reference localities in norway. | the main goals of this study were to define, for the norwegian coast, "high background" levels of micropollutants routinely monitored within the the oslo and paris commission joint assessment and monitoring programme, and to reassess the present norwegian reference levels used as the basis for a classification of environmental quality. the indicator organisms analysed as bulked/individual samples were cod (gadus morhua, n approximately 1170; n approximately 240 for organochlorines), dab (limanda ... | 2003 | 12604072 |
| prevalence of viral haemorrhagic septicaemia virus in danish marine fishes and its occurrence in new host species. | in order to analyse the occurrence of viral haemorrhagic septicaemia virus (vhsv) in the marine waters around denmark, staff from the danish institute for food and veterinary research participated in 5 research cruises during 1998 to 2002 as a follow-up to 4 research cruises performed in 1996 to 1997. in total, 16,655 fish were examined virologically as 3569 samples. forty fish species and 3 invertebrate species were represented. vhsv was isolated from 133 samples representing 8 species: herring ... | 2005 | 16231640 |
| isolation of the retinoblastoma cdna from the marine flatfish dab (limanda limanda) and evidence of mutational alterations in liver tumors. | we have isolated a dab (limanda limanda) homologue of the human retinoblastoma (rb) tumor suppressor gene. the l. limanda partial rb cdna encodes a partial predicted protein of 753 amino acids. dna sequence analysis with other vertebrate rb sequences demonstrates that the l. limanda rb cdna is highly conserved in regions of functional importance. the sequence reported herein, combined with the high degree of conservation observed in critical domains, has also facilitated an investigation of the ... | 2005 | 16475367 |
| stable expression of bioactive recombinant pleurocidin in a fish cell line. | pleurocidin (ple), a linear cationic peptide of 25 amino acids, is a member of a larger family of antimicrobial peptides present in flatfish. previous studies have shown that ple displays a strong antimicrobial activity against a broad spectrum of bacteria and appears to play a role in innate host defence. in this work, the genomic sequence encoding the ple prepropeptide has been isolated from limanda limanda and cloned in a vector under the control of a non-viral promoter (the carp beta-actin p ... | 2006 | 16636829 |
| can the dab (limanda limanda) be a paratenic host of anguillicola crassus (nematoda; dracunculoidea)? the gulf of gdańsk and vistula lagoon (poland) example. | anguillicola crassus is an asian nematode, dwelling in eel swim bladder and accidentally introduced to europe. the eel becomes infected by consuming either crustaceans (intermediate hosts) or small fish (paratenic hosts), supporting stage 3 larvae. in 2002, 15 specimens of dab (limanda limanda) caught in the gulf of gdarisk and 8 caught in the vistula lagoon were examined for the presence ofa. crassus larvae. two gulf of gdatisk specimens and one vistula lagoon fish were found to host 4 stage 3 ... | 2004 | 16859042 |
| parvicapsula bicornis n. sp. and p. limandae n. sp. (myxozoa, parvicapsulidae) in pleuronectidae (teleostei, heterosomata) from denmark. | two species of parvicapsula were found in the kidney tubules and the urinary bladder of 2 pleuronectid fish from the northern oresund, denmark. the coelozoic, spherical, disporic trophozoites of both species are 10 to 12 pm in diameter. the myxospores of both species are elongate, asymmetrical and slightly curved, and have spherical polar capsules. parvicapsula bicornis n. sp. (6-8 x 5-6 microm, polar capsule 2.5 microm in diameter) occurs in pleuronectes platessa. the polar capsules of p. bicor ... | 2007 | 17760385 |
| distribution of infectious pancreatic necrosis virus (ipnv) in wild marine fish from scottish waters with respect to clinically infected aquaculture sites producing atlantic salmon, salmo salar l. | this study represents the first large-scale investigation of ipnv in scottish wild marine fish. kidney samples were taken from 30 627 fish comprising 37 species and 45 isolations were made from nine different species, illustrating these as reservoirs of ipnv in scottish waters. the estimated prevalence of ipnv in the scottish marine environment was low at 0.15% (90% confidence intervals, (ci) of 0.11-0.19%). this was significantly greater in fish caught less than 5.0 km from ipn-positive fish fa ... | 2008 | 18261031 |
| a novel population health approach: using fish retinoblastoma gene profiles as a surrogate for humans. | retinoblastoma, a tumor suppressor gene, is frequently mutated in diverse types of human tumors. we have previously shown that two types of fish tumor, eye and liver, also possess mutant rb genes. our aim is to determine if the rb allele status is linked to environmentally-induced cancer and whether this information in fish can be used to predict future phenotype. this is a proof-of-concept investigation to elucidate if fish may act as surrogates in assessing pollution-induced tumor incidence an ... | 2009 | 18835587 |
| biological effect monitoring in dab (limanda limanda) using gene transcript of cyp1a1 or erod-a comparison. | gene expression analyses with real-time (rt)-polymerase chain reaction (pcr) gains importance in marine monitoring. this new technique has to be compared to the classical approaches like the well known biomarker ethoxyresorufin-o-deethylase (erod) to test their suitability for monitoring programmes. the goal of the present study is to compare erod activity and cyp1a1 mrna expression in the important monitoring fish species dab (limanda limanda) and to answer the question of whether these paramet ... | 2008 | 18853211 |
| long-term trends in the prevalence of cancer and other major diseases among flatfish in the southeastern north sea as indicators of changing ecosystem health. | this paper analyses and discusses spatial and temporal patterns in the prevalence of major skin diseases (lymphocystis, epidermal hyperplasia/papilloma, ulcers), intestinal parasite glugea sp., and liver cancer in dab (limanda limanda) and flounder (platichthys flesus) in the dutch section of the north sea since the mid-1980s. we have attempted to relate disease prevalence trends in both species to chemical contaminant exposure and other relevant environmental factors including fish condition fa ... | 2009 | 19368228 |
| detection of salmonid alphavirus rna in wild marine fish: implications for the origins of salmon pancreas disease in aquaculture. | salmonid alphaviruses (savs), which include the aetiological agents of salmon pancreas disease (spd) in farmed atlantic salmon salmo salar l. and sleeping disease (sd) in rainbow trout oncorhynchus mykiss (walbaum), are significant viral pathogens of european salmonid aquaculture. sav is horizontally transmitted and the virus can survive for extended periods in seawater. a lack of convincing evidence for vertical transmission coupled to the fact that the spd virus (spdv) occurs in historically i ... | 2010 | 21133318 |
| molecular identification and transmission studies of x-cell parasites from atlantic cod gadus morhua (gadiformes: gadidae) and the northern black flounder pseudopleuronectes obscurus (pleuronectiformes: pleuronectidae). | epidermal pseudotumours from hippoglossoides dubius and acanthogobius flavimanus in japan and gill lesions in limanda limanda from the uk have been shown to be caused by phylogenetically related protozoan parasites, known collectively as x-cells. however, the phylogenetic position of the x-cell group is not well supported within any of the existing protozoan phyla and they are currently thought to be members of the alveolata.ultrastructural features of x-cells in fish pseudotumours are somewhat ... | 2011 | 21299903 |
| field studies of hearing in two species of flatfish pleuronectes platessa (l.) and limanda limanda (l.) (family pleuronectidae). | | 2014 | 4149016 |
| diet overlap among flatfish species in the southern north sea. | the diets of two non-commercial flatfish species (solenette buglossidium luteum and scaldfish arnoglossus laterna) and two commercial flatfish species (dab limanda limanda and plaice pleuronectes platessa) were compared in a study area in the german bight (southern north sea) to investigate prey-resource partitioning between these species. the diets of a. laterna and b. luteum mainly comprised crustaceans (harpacticoids, amphipods, cumaceans and decapods), whereas the diet of l. limanda and p. p ... | 2012 | 22650434 |
| molecular biomarkers: their significance and application in marine pollution monitoring. | this paper presents an overview of the significance of the use of molecular biomarkers as diagnostic and prognostic tools for marine pollution monitoring. in order to assess the impact of highly persistent pollutants such as polychlorinated biphenyls (pcb), polychlorinated dibenzo-dioxins (pcdd), polychlorinated dibenzo-furans (pcdf), polynuclear aromatic hydrocarbons (pah), tributyltin (tbt) and other toxic metals on the marine ecosystem a suite of biomarkers are being extensively used worldwid ... | 2006 | 16676218 |
| environmental impact and recovery at two dumping sites for dredged material in the north sea. | the environmental impact and recovery associated with the long and uninterrupted disposal of large volumes of moderately contaminated dredged material from the port of rotterdam was studied at nearby dumping sites in the north sea. observations were made on sediment contamination, ecotoxicity, biomarker responses and benthic community changes shortly after dumping at the 'north' site had ceased and at the start of disposal at the new dumping site 'northwest'. during the period of dumping, very f ... | 2003 | 12683979 |
| molecular responses as indicators of marine pollution: dna damage and enzyme induction in limanda limanda and asterias rubens. | during a survey from 26 august through 13 september 1991, specimens of the flatfish, limanda limanda (dab), and the asteroid echinoderm asterias rubens (seastar), were collected at sampling locations along transects radiating into the north sea from the coastal zone of the netherlands. in homogenates of liver tissue from male dab and the digestive gland (pyloric caeca) of female seastar, dna damage (strand breaks) and induction of the cytochrome p450-dependent monooxygenase system (mo) were dete ... | 1994 | 7713032 |
| influences of potential predictor variables on gastric evacuation in atlantic cod gadus morhua feeding on fish prey: parameterization of a generic model. | the parameter values of a generic model of gastric evacuation were estimated from evacuation data on atlantic cod gadus morhua fed meals of four fish prey: herring clupea harengus, sprat sprattus sprattus, lesser sandeel ammodytes tobianus and dab limanda limanda. the effects on evacuation of photoperiod and pre-experimental treatment of prey were also tested. freshly killed a. tobianus were evacuated from the stomach of g. morhua at a rate similar to the value estimated from conspecifics kept d ... | 2012 | 22380555 |
| a permanent fish cell line (epc) for genotoxicity testing of marine sediments with the comet assay. | genotoxicity and cytotoxicity were evaluated in an in vitro system with a permanent cell line epithelioma papulosum cyprini (epc) derived from a skin tumour of carp (cyprinus carpio l.). epc cells were exposed to different concentrations of organic sediment extracts from the north sea for 24h. after incubation the cells were analysed for viability and dna strand breaks with the comet assay or single cell gel electrophoresis (scge). the results confirm the sensitivity of this assay. out of 10 mar ... | 2001 | 11673072 |
| does the cylinder model of gastric evacuation predict observed evacuation of mixed meals of prey of contrasting geometries in a piscivorous fish? | the simple surface abstraction of the cylinder model (each prey as well as the total stomach contents is considered a cylinder that is gradually reduced by successive peeling off its curved side) was challenged by data on evacuation of a meal composed of three sandeels ammodytes tobianus and a dab limanda limanda fed to atlantic cod gadus morhua. while the body shape of a. tobianus comes close to that of a cylinder, the flatfish l. limanda takes a discoid form. as opposed to a modified form of t ... | 2012 | 22220896 |
| salinity gradient shapes distance decay of similarity among parasite communities in three marine fishes. | published data were used to compare the distance decay of similarity in parasite communities of three marine fish hosts: atlantic cod gadus morhua, the dab limanda limanda and the flounder platichthys flesus in two adjacent areas that differ with respect to the strength of a salinity gradient. in the baltic sea, which exhibits a strong salinity gradient from its connection with the north sea in the west to its head in the north-east, parasite communities in all three fish hosts showed a signific ... | 2010 | 20557632 |
| environmental genotoxicity and cytotoxicity in the offshore zones of the baltic and the north seas. | micronuclei (mn), nuclear buds (nb) and fragmented-apoptotic cells (fa) were analyzed in mature peripheral blood and immature cephalic kidney erythrocytes of flounder (platichthys flesus), dab (limanda limanda) and cod (gadus morhua) from 12 offshore sites in the baltic sea (479 specimens) and 11 sites in the north sea (291 specimens), which were collected during three research vessel cruises in december 2002, 2003 and in september 2004. the highest levels of environmental genotoxicity (frequenc ... | 2009 | 19616842 |
| integrated monitoring of chemicals and their effects on four sentinel species, limanda limanda, platichthys flesus, nucella lapillus and mytilus sp., in seine bay: a key step towards applying biological effects to monitoring. | the international workshop on integrated assessment of contaminants impacts on the north sea (icon) provided a framework to validate the application of chemical and biological assessment thresholds (bacs and eacs) in the seine bay in france. bioassays (oyster larval anomalies, corophium arenarium toxicity assay and dr calux) for sediment and biomarkers: ethoxyresorufin-o-deethylase (erod) activity, acetylcholinesterase (ache) activity, lysosomal membrane stability (lms), dna strand breaks using ... | 2017 | 27839718 |