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prebiotic effects of arabinoxylan oligosaccharides on juvenile siberian sturgeon (acipenser baerii) with emphasis on the modulation of the gut microbiota using 454 pyrosequencing.the potential of a novel class of prebiotics, arabinoxylan oligosaccharides (axos), was investigated on growth performance and gut microbiota of juvenile acipenser baerii. two independent feeding trials of 10 or 12 weeks were performed with basal diets supplemented with 2% or 4% axos-32-0.30 (trial 1) and 2% axos-32-0.30 or axos-3-0.25 (trial 2), respectively. growth performance was improved by feeding 2% axos-32-0.30 in both trials, although not significantly. microbial community profiles were ...201323786549
evolutionary aspects of intestinal bicarbonate secretion in fish.experiments compared intestinal hco3- secretion in the intestine of marine teleost gulf toadfish, opsanus beta, to representatives of early chondrostean and chondrichthyan fishes, the siberian sturgeon, acipenser baerii, and white-spotted bamboo shark, chiloscyllium plagiosum, respectively. as seen in marine teleosts, luminal hco3- concentrations were 10-fold plasma levels in all species when exposed to hyperosmotic conditions. while intestinal water absorption left mg2+ and so4(2-) concentrated ...200616503178
partial genome analysis of siberian sturgeon alloherpesvirus suggests its close relation to acihv-2 - short communication.partial genome sequence of a herpes-like virus, isolated from siberian sturgeon (acipenser baeri), was determined and subjected to phylogenetic analysis. the virus (sbshv) has been shown to be the causative agent of an acute disease with high mortality in farmed juvenile sturgeons in russia. two fragments (of 7000 and 300 base pairs in length) encompassing 3 complete and 3 partial orfs were amplified by pcr. sturgeon herpesvirus strains, classified into species acipenserid herpesvirus 2 (acihv-2 ...201020460226
phb-degrading bacteria isolated from the gastrointestinal tract of aquatic animals as protective actors against luminescent vibriosis.the use of poly-β-hydroxybutyrate (phb) was shown to be successful in increasing the resistance of brine shrimp against pathogenic infections. in this study, we isolated for the first time phb-degrading bacteria from a gastrointestinal environment. pure strains of phb-degrading bacteria were isolated from siberian sturgeon, european sea bass and giant river prawn. the capability of selected isolates to degrade phb was confirmed in at least two of three setups: (1) growth in minimal medium contai ...201020597982
glyconjugates in epidermal, branchial and digestive mucous cells and gastric glands of gilthead sea bream, sparus aurata, senegal sole, solea senegalensis and siberian sturgeon, acipenser baeri development.epidermal, branchial and digestive mucous cells, and the gastric glands of larvae/postlarvae (from hatching until 45 days posthatching) of three fish species (two teleostean and a chondrostean) were investigated using conventional histochemical methods (periodic acid schiff -pas-, diastase-pas; alcian blue ph 0.5, 1 and 2.5) in order to distinguish neutral and acidic (carboxylated and sulphated) glycoconjugates, as well as bromophenol blue reaction for identification of proteins. additionally, t ...200111759813
comparative distribution of mammalian gnrh (gonadotrophin-releasing hormone) and chicken gnrh-ii in the brain of the immature siberian sturgeon (acipenser baeri).the brain of the sturgeon has recently been shown to contain at least two forms of gnrh (gonadotropin-releasing hormone), mammalian gnrh (mgnrh) and chicken gnrh-ii (cgnrh-ii). in this study, we compared the distribution of immunoreactive (ir) mgnrh and cgnrh-ii in the brain of immature siberian sturgeons (acipenser baeri). the overall distribution of mgnrh was very similar to the distribution of sgnrh in teleosts such as salmonids or cyprinids. mgnrh-ir perikarya were observed in the olfactory ...19938288771
disposition of genistein in rainbow trout (oncorhynchus mykiss) and siberian sturgeon (acipenser baeri).genistein (g) is a xenoestrogen from soy present in fish diet. in vivo, a 50-fold difference in sensitivity to genistein on vitellogenin (vtg) synthesis was found when comparing trout and sturgeon. this difference was not linked to the estrogen receptor affinity nor to the sensitivity of induction of the vtg pathway. the study was performed to check if differences in the g disposition in the two species could explain their difference of sensitivity to g. a pharmacokinetic analysis of radiolabele ...200717097654
previtellogenic and vitellogenic oocytes in ovarian follicles of cultured siberian sturgeon acipenser baerii (chondrostei, acipenseriformes).previtellogenic and vitellogenic oocytes in ovarian follicles from cultured siberian sturgeon acipenser baerii were examined. in previtellogenic oocytes, granular and homogeneous zones in the cytoplasm (the ooplasm) are distinguished. material of nuclear origin, rough endoplasmic reticulum, golgi complexes, complexes of mitochondria with cement and round bodies are numerous in the granular ooplasm. in vitellogenic oocytes, the ooplasm comprises three zones: perinuclear area, endoplasm and peripl ...201727859485
variability of the immunoglobulin light chain in the siberian sturgeon, acipenser baeri.all sturgeon vl segments isolated in this study belong to a single family, vli, which can be divided into two subfamilies. of the 79 cdna clones isolated, 76 belong to the larger subfamily, vlia, and only 3 clones constitute the smaller subfamily, vlib. to evaluate variability, the shannon entropy was estimated for each individual amino acid position, and to facilitate comparisons of variability between species the mean entropy of the cdr regions was calculated. in such a comparison, the sturgeo ...199910579389
hemato-immunological responses and disease resistance in siberian sturgeon acipenser baerii fed on a supplemented diet of lactobacillus plantarum.a feeding trial was conducted to investigate the effects of different levels of dietary lactobacillus plantarum on hemato-immunological parameters and resistance against streptococcus iniae infection in juvenile siberian sturgeon acipenser baerii. fish (14.6 ± 2.3 g) were fed three experimental diets prepared by supplementing a basal diet with l. plantarum at different concentrations [1 × 10(7), 1 × 10(8) and 1 × 10(9) colony-forming units (cfu) g(-1)] and a control (non-supplemented basal) diet ...201727619367
characterization of proteolytic and anti-proteolytic activity involvement in sterlet spermatozoon maturation.in sturgeon, the acquisition of the potential for motility activation called spermatozoon maturation takes place outside testes. this process can be accomplished in vitro by pre-incubation of immature testicular spermatozoa in seminal fluid collected from fully mature wolffian duct sperm. addition of trypsin inhibitor to the pre-incubation medium disrupts spermatozoon maturation. there are no available data for the role of proteolysis regulators in fish spermatozoon maturation, while their role ...201627401784
expression of growth hormone gene during early development of siberian sturgeon (acipenserbaerii).the mrna expression of growth hormone (gh) gene in early development stages of siberian sturgeon was investigated using rt-pcr method. samples were collected from unfertilized eggs up to 50 days post hatched (dph) larvae in 11 different times. ribosomal protein l6 (rpl6) transcripts were used as the internal standard during quantification of gh mrna expression. the results showed that the gh mrna could be observed in the eyed eggs and even at unfertilized eggs of siberian sturgeon. the highest a ...201527844010
fertilization success of sterlet acipenser ruthenus and siberian sturgeon acipenser baerii gametes under conditions of heterospecific mating.species may be prevented from interspecific hybridization by a number of different reproductive barriers that operate precopulatory and postcopulatory. in situation, when natural precopulatory reproductive barriers are affected by anthropogenic factors, postcopulatory reproductive barriers may be important for maintaining gametic isolation and hence preventing interspecific hybridization. this is highly topical in sturgeon (order acipenseriformes) which exhibits remarkable ease of interspecific ...201627692499
age and heat stress as determinants of telomere length in a long-lived fish, the siberian sturgeon.telomeres shorten at each cell division due to the end-replication problem but also in response to oxidative stress. consequently, telomeres shorten with age in many endotherms, and this shortening is accelerated under stressful environmental conditions. data in ectotherm vertebrates remain scarce so far, so our goal was to review existing data for fish and to test the influence of age and stress on telomere length in a very long-lived fish, the siberian sturgeon (acipenser baerii). our review o ...201627617363
Effects of poly-ß-hydroxybutyrate (PHB) on Siberian sturgeon (Acipenser baerii) fingerlings performance and its gastrointestinal tract microbial community.Poly-ß-hydroxybutyrate (PHB) is a natural polymer that can be depolymerized into water-soluble short-chain fatty acid monomers. These monomers can act as microbial control agents. In this study, the effects of partially replacing the diet of Siberian sturgeon fingerlings with 2% and 5% PHB were investigated. Replacing 2% of the diet with PHB improved weight gain, specific growth rate (SGR) and survival in the sturgeon fingerlings during the 10-week experimental period. Community-level physiologi ...201122066757
effect of poly-β-hydroxybutyrate on growth and disease resistance of nile tilapia oreochromis niloticus juveniles.the growth promoting effect of the bacterial storage compound poly-β-hydroxybutyrate (phb) has been studied for young fish of high trophic level (european sea bass) and intermediate trophic level (siberian sturgeon). here, the effect of phb on growth, digestive enzyme activities, body composition and diseases resistance of juvenile nile tilapia (oreochromis niloticus) of low trophic level was investigated. although dietary phb supplementation (5, 25 and 50 g phb kg(-1) formulated semi-purified d ...201626711027
cloning hsp70 and hsp90 genes of kaluga (huso dauricus) and the effects of temperature and salinity stress on their gene expression.the genes encoding hsp70 and hsp90 proteins were isolated from kaluga by homologous cloning and rapid amplification of complementary dna (cdna) ends (race). hsp70 (genbank accession no. kp050541) and hsp90 (genbank accession no. kp050542) cdnas were composed of 2275 and 2718 bp and encoded polypeptides of 650 and 725 amino acids, respectively. basic local alignment search tool (blast) analysis showed that hsp70 and hsp90 of kaluga shared high identities with those of acipenser ruthenus, acipense ...201626683614
first detection of a viral agent causing disease in farmed sturgeon in russia.a virus was isolated from siberian sturgeon acipenser baeri and bester (beluga huso huso x sterlet a. ruthenus hybrid) fingerlings in sso-2, ssf-2 and wssk-1 cell lines during an acute epizootic on a large fish farm producing fertilised sturgeon eggs and fry. transmission electron microscopic examination of samples from both inoculated cell cultures and skin of affected fish revealed viral particles with a herpesvirus-like morphology. the etiological role of the siberian sturgeon herpesvirus (sb ...200920066954
malformations of the endangered chinese sturgeon, acipenser sinensis, and its causal agent.the anadromous chinese sturgeon (acipenser sinensis) is endangered and listed among the first class of protected animals in china. the possible causes for the decline of this species are the effects of synthetic chemicals, and loss of critical habitat. chinese sturgeon in the yangtze river have accumulated triphenyltin (tpt) to 31-128 ng/g wet weigh (ww) in liver, which is greater than the concentrations of tributyltin (<1.0 ng/g ww). maternal transfer of tpt has resulted in concentrations of 25 ...200919470453
dietary administration of lactobacillus plantarum enhanced growth performance and innate immune response of siberian sturgeon, acipenser baerii.we investigated the effects of lactobacillus plantarum used as a dietary supplement on the growth performance and innate immune response in juvenile siberian sturgeon acipenser baerii. juvenile fish (14.6 ± 2.3 g) were fed three experimental diets prepared by supplementing a basal diet with l. plantarum at different concentrations [1 × 10(7), 1 × 10(8) and 1 × 10(9) colony-forming units (cfu) g(-1)] and a control (non-supplemented basal) diet for 8 weeks. growth performance indices were increase ...201626686864
effects of dietary arabinoxylan-oligosaccharides (axos) and endogenous probiotics on the growth performance, non-specific immunity and gut microbiota of juvenile siberian sturgeon (acipenser baerii).we investigated the effects of administration of putative endogenous probiotics lactococcus lactis spp. lactis or bacillus circulans, alone and in combination with arabinoxylan-oligosaccharides (axos), a new class of candidate prebiotics, in juvenile siberian sturgeon (acipenser baerii). eight experimental diets were tested: basal diet (diet 1), basal diet supplemented with 2% axos (diet 2), or l. lactis st g81 (diet 3), l. lactis st g45 (diet 4), b. circulans st m53 (diet 5), l. lactis st g81 + ...201323811408
functional c1q is present in the skin mucus of siberian sturgeon (acipenser baerii).the skin mucus of fish acts as the first line of self-protection against pathogens in the aquatic environment and comprises a number of innate immune components. however, the presence of the critical classical complement component c1q, which links the innate and adaptive immune systems of mammalians, has not been explored in a primitive actinopterygian fish. in this study, we report that c1q is present in the skin mucus of the siberian sturgeon (acipenser baerii). the skin mucus was able to inhi ...201524920077
effects of propofol and carbon dioxide on acid-base balance in siberian sturgeon.the aim of this study was to compare physiological responses in siberian sturgeon (acipenser baeri) induced by propofol and co2 anaesthesia. two procedures were applied during the experiment. in procedure i, blood samples were collected immediately after exposure (1, 2, 5, 10 min) to the anaesthetic. in procedure ii, fish were exposed to the anaesthetic for 10 minutes and then were moved to anaesthetic free water. blood was sampled after 5, 10, 20 or 30 min of recovery time. gasometrical and bio ...201526172175
intraperitoneal injection urocortin-3 reduces the food intake of siberian sturgeon (acipenser baerii).urocortin-3 (ucn3), one of the corticotropin releasing factor (crf) family peptides, which was discovered in 2001, has a variety of biological functions. however, the researches of ucn3 in fish were scarce. in order to understand whether ucn3 play a role in regulating food intake in fish, we first cloned the ucn3 cdnas sequence of siberian sturgeon (acipenser baerii brandt), and investigated the ucn3 mrna levels in 11 tissues. the siberian sturgeon ucn3 cdna sequence was 1044bp, including an ope ...201627667703
immunocytochemical reactivity of rod and cone visual pigments in the sturgeon retina.microspectrophotometry and immunocytochemistry with several antivisual pigment antibodies were used to study visual cells of the siberian sturgeon, acipenser baeri brandt. the retina contained rods and three morphological types of cones: large cones with oil drops, small cones with oil drops, and cone-like cells without oil drops. rods and cone-like drop-free cells were found to possess porphyropsin-549, while the large oil drop-bearing cones contained red-sensitive (p613), green-sensitive (p542 ...19921534023
new extender for cryopreservation of siberian sturgeon (acipenser baerii) semen.the goal of this study was to develop a simple glucose-methanol extender for cryopreservation of siberian sturgeon (acipenser baerii) semen. semen quality was assessed by determining post-thaw sperm motility and fertilizing ability at hatching stage. we tested the effect of glucose concentration (0, 0.10, 0.15, 0.20 and 0.30 m) in a methanol extender on post-thaw sperm motility. sperm motility parameters and fertilizing ability of semen cryopreserved in 0.1 m glucose in 15% methanol (gm) were co ...201525725469
coamplification of eukaryotic dna with 16s rrna gene-based pcr primers: possible consequences for population fingerprinting of complex microbial communities.the main aim of this study was to evaluate the specificity of three commonly used 16s rrna gene-based polymerase chain reaction (pcr) primer sets for bacterial community analysis of samples contaminated with eukaryotic dna. the specificity of primer sets targeting the v3, v3-v5, and v6-v8 hypervariable regions of the 16s rrna gene was investigated in silico and by community fingerprinting of human and fish intestinal samples. both in silico and pcr-based analysis revealed cross-reactivity of the ...200818301945
electrical excitability of the heart in a chondrostei fish, the siberian sturgeon (acipenser baerii).sturgeon (family acipenseridae) are regarded as living fossils due to their ancient origin and exceptionally slow evolution. to extend our knowledge of fish cardiac excitability to a chondrostei fish, we examined electrophysiological phenotype of the siberian sturgeon (acipenser baerii) heart with recordings of epicardial ecg, intracellular action potentials (aps), and sarcolemmal ion currents. epicardial ecg of a. baerii had the typical waveform of the vertebrate ecg with q-t interval (average ...201425163915
effect of diet composition on growth performance, hepatic metabolism and antioxidant activities in siberian sturgeon (acipenser baerii, brandt, 1869) submitted to starvation and refeeding.many fish species undergo natural starvation periods. adaptation to starvation is possible through the activation of behavioral, biochemical and physiological mechanisms. knowledge of the effect of dietary nutrients on the intermediary metabolism during starvation and refeeding can be useful to improve fish health and optimize aquaculture production. to analyze the effect of dietary nutrients on liver metabolism of siberian sturgeon (acipenser baerii) submitted to starvation and refeeding, four ...201627216494
cryopreservation of early stage siberian sturgeon acipenser baerii germ cells, comparison of whole tissue and dissociated cells.several sturgeon species are near extinction; therefore an efficient conservation strategy is required. germ stem cells can be used for long-term storage and restoration of genetic information using surrogate reproduction. this study compared cryopreservation procedures of early stages of siberian sturgeon acipenser baerii testicular and ovarian cells. whole gonad tissue or dissociated cells were frozen at a cooling rate of 1 °c/min in phosphate buffered saline with 0.5% bovine serum albumin, 50 ...201626920821
the second highest chromosome count among vertebrates is observed in cultured sturgeon and is associated with genome plasticity.one of the five basal actinopterygian lineages, the chondrostei, including sturgeon, shovelnose, and paddlefish (order acipenseriformes) show extraordinary ploidy diversity associated with three rounds of lineage-specific whole-genome duplication, resulting in three levels of ploidy in sturgeon. recently, incidence of spontaneous polyploidization has been reported among cultured sturgeon and it could have serious negative implications for the economics of sturgeon farming. we report the occurren ...201626867760
variations of some physiological and immunological parameters in siberian sturgeon (acipenser baerii, brandt, 1869) subjected to an acute stressor.this study was carried out to investigate the effect of an acute stressor on the variation of some physiological and immunological parameters of siberian sturgeon (acipenser baerii) juveniles. fish, reared in 3 tanks for 10 weeks, were used for this study. the acute stress of fish consisted of 2 min of air exposure stress. plasma levels of cortisol, glucose, and lactate as well as lysozyme activity in plasma were measured before stress and 1 hr, 3 hr, 6 hr, 9 hr, 12 hr, and 24 hr after stress. t ...201424484309
neomycin damage and regeneration of hair cells in both mechanoreceptor and electroreceptor lateral line organs of the larval siberian sturgeon (acipenser baerii).the lateral line found in some amphibians and fishes has two distinctive classes of sensory organs: mechanoreceptors (neuromasts) and electroreceptors (ampullary organs). hair cells in neuromasts can be damaged by aminoglycoside antibiotics and they will regenerate rapidly afterward. aminoglycoside sensitivity and the capacity for regeneration have not been investigated in ampullary organs. we treated siberian sturgeon (acipenser baerii) larvae with neomycin and observed loss and regeneration of ...201626502298
dietary ascorbic acid may be necessary for enhancing the immune response in siberian sturgeon (acipenser baerii), a species capable of ascorbic acid biosynthesis.siberian sturgeon (acipenser baerii brandt) is one of few fish species capable of synthesizing ascorbic acid (aa); however, it is unknown whether dietary aa is still necessary for this species to generate an optimal non-specific immune response, as is found in most teleostean species that do not synthesize aa. sturgeon fed either 0 or 500 mg aa (aa polyphosphate) equivalent kg(-1) diets for 2 weeks were then either sham or experimentally injected with aeromonas hydrophila lipopolysaccharide (lps ...200616901741
outbreak of mortality in russian (acipenser gueldenstaedtii) and siberian (acipenser baerii) sturgeons associated with sturgeon nucleo-cytoplasmatic large dna virus.diseased outbreaks with high mortality in farmed sturgeon are a limiting factor to the success of this emerging aquaculture sector in europe. thorough investigations of outbreaks can determine the aetiological agents, identify important pathological and epidemiological pathways of infections and pave the way for effective control strategies. a thorough investigation of a mortality outbreak in russian (acipenser gueldenstaedtii) and siberian (acipenser baerii) sturgeons in italy, demonstrated the ...201627374904
availability of in vitro vitellogenin assay for screening of estrogenic and anti-estrogenic activities of environmental chemicals.vitellogenin (vtg) protein, vtg mrna, other egg yolk proteins, vitelline envelope proteins and their mrnas are produced in the liver of oviparous species by stimulation of endogenous estrogen and exogenous estrogenic chemicals. the vtg assay based on enzyme-linked immunosorbent assay (elisa) has been widely used for many fish species to screen estrogenic and anti-estrogenic activities of chemicals and sewage effluents using immature fish and/or male fish. in order to reduce the number of fish fo ...200616883298
induction of meiotic gynogenesis in ship sturgeon acipenser nudiventris using uv-irradiated heterologous sperm.diploid gynogenesis was induced in ship sturgeon acipenser nudiventris using uv-irradiated sperm from siberian sturgeon acipenser baerii. the optimal condition for the retention of the second polar body in ship sturgeon was determined to be 10 min after activation/fertilization in experiments. the temperature of cold shock and its duration were 2.5 °c and 30 min, respectively. a total of 30 gynogens of known parentage from experimental treatments were screened using microsatellite dna analysis, ...201424338125
[intraspecific variability of life strategies in boreal fishes inhabiting wide ranges].variability of the population structure, parameters of growth, and reproduction were analyzed in two freshwater (siberian sturgeon and bream) and two marine (cod and flounder) widespread boreal fish species. these species have similar spectrums of adaptive life strategies including (a) the strategy of populations inhabiting zones of the species optimum, (b) the strategy of populations inhabiting the northern peripheral zones of low temperature and unstable food supply, (c) the strategy of popula ...201620799650
development of the skull and pectoral girdle in siberian sturgeon, acipenser baerii, and russian sturgeon, acipenser gueldenstaedtii (acipenseriformes: acipenseridae).the head is considered the major novelty of the vertebrates and directly linked to their evolutionary success. its form and development as well as its function, for example in feeding, is of major interest for evolutionary biologists. in this study, we describe the skeletal development of the cranium and pectoral girdle in siberian (acipenser baerii) and russian sturgeon (a. gueldenstaedtii), two species that are commonly farmed in aquaculture and increasingly important in developmental studies. ...201728176372
[isolation and identification of pathogenic aeromonas veronii isolated from infected siberian sturgeon (acipenser baerii)].our study aimed at searching for the pathogenic factor causing siberian sturgeon (acipenser baerii) disease.200920069873
investigation of mucus obtained from different fish species on the acute pain induced with scalpel incision in paw of rats.no comparative study could be found for the analgesic activity of mucuses from the oncorhynchus mykiss (om), salvelinus fontinalis (sf), salmo coruhensis (sc), acipenser gueldenstaedtii (ag), and acipenser baerii (ab) fish species in the literature. we aimed to investigate the effects of mucuses obtained from the abovementioned fish species on scalpel incision-induced pain in the rat paw and to examine the role of oxidant/antioxidant parameters and cox-2 gene expression in the analgesic activiti ...201626490740
identification of the molecular sex-differentiation period in the siberian sturgeon.sexual development prior to gonadal sex differentiation is regulated by various molecular mechanisms. in fish, a "molecular sex-differentiation period" has been identified in species for which sex can be ascertained prior to gonadal sex differentiation. the present study was designed to identify such a period in a species for which no genetic sex markers or monosex populations are available. siberian sturgeons undergo a slow sex-differentiation process over several months, so gonad morphology an ...201626461178
first detection of pike fry-like rhabdovirus in barbel and spring viraemia of carp virus in sturgeon and pike in aquaculture in the czech republic.rapid antigen detection enzyme-linked immunosorbent assay (elisa) testing of cell cultures with organ homogenate from fish, collected from farms with a predominance of common carp or in natural aquaculture in the czech republic between 1995 and 2008, identified piscine vesiculovirus in 27 of 178 samples. using reverse transcription semi-nested pcr, targeting a 550 nucleotide region of the glycoprotein (g) gene, piscine vesiculovirus was confirmed in 23 of the 27 organ samples diagnosed by elisa ...201121848117
technical note: production of tetraploid sturgeons.studies and practical application of androgenesis and gynogenesis in sturgeon are significantly hindered by strong influence of ploidy restoration treatment on survivability of progeny; therefore, developed method of production of tetraploid broodstock and, consequently, use of their diploid gametes might help to avoid ploidy restoration treatment. in the present study, for the first time was developed a protocol for tetraploidy induction in 2 model sturgeon species, sterlet () and siberian stur ...201526440154
rule of accumulation of enrofloxacin in acipenser baerii and drug-induced damage to the tissues.enrofloxacin (enx) has been widely used in the prevention and control of bacterial diseases in sturgeon aquaculture due to its characteristics of wide antibacterial spectrum, strong antibacterial activity, less toxicity and fewer side effects, rapid action, extensive in vivo distribution, and little cross-resistance with other antibiotics. however, the spinal abnormality was found in acipenser baerii soon after enx administration, which resulted an "s"-shaped curvature of the spine and retarded ...201627296849
histopathological effects of silver and copper nanoparticles on the epidermis, gills, and liver of siberian sturgeon.the influence of nanoparticles (nps) on aquatic environments is still poorly documented. the aim of the study was to determine the effects of silver (agnps) and copper (cunps) nanoparticles on larval siberian sturgeon (acipenser baerii) after 21 days of exposure. acute toxicity of agnps on siberian sturgeon was investigated in a 96-h static renewal study and compared with the toxicity of cunps. the agnps and cunps 96 h mean lethal concentrations (96 h lc50) were 15.03 ± 2.91 and 1.41 ± 0.24 mg l ...201626381783
molecular cloning, expression analysis, and appetite regulatory effect of peptide yy in siberian sturgeon (acipenser baerii).peptide yy (pyy) is an anorectic brain-gut peptide involved in feeding regulation and well characterized in mammals. however, the functional role of pyy in the appetite regulatory of fish is not clear. in this study, we characterized a high conservation of pyy cdna and found high expression levels of pyy mrna in the brain and digestive tract of siberian sturgeon. then, we examined preprandial (pre- and post-feeding) changes of pyy mrna expression in the brain that showed a significantly increase ...201525791494
effects of the supplementation of vitamin d3 on the growth and vitamin d metabolites in juvenile siberian sturgeon (acipenser baerii).the objective of this study was to evaluate the effects of the supplementation of vitamin d3 on the growth, vitamin d metabolites, and osteocalcin secretion in juvenile siberian sturgeon (acipenser baerii). a 90-day growth trial was conducted with juvenile siberian sturgeon (initial body weight 3.47 ± 0.14 g) fed seven isonitrogenous and isoenergetic practical diets (45% cp and 13% lipid) containing 60 (basal diet), 240, 450, 880, 1670, 3300, or 1.0 × 10(5) iu/kg feed (d60~d 1.0 × 10(5)) vitamin ...201728116596
evolutionary relations and population differentiation of acipenser gueldenstaedtii brandt, acipenser persicus borodin, and acipenser baerii brandt.russian ( acipenser gueldenstaedtii), persian ( a. persicus) and siberian ( a. baerii) sturgeons are closely related 'ponto-caspian' species. investigation of their population structure is an important problem, the solution of which determines measures for conservation of these species. according to previous studies, 'baerii-like' mitochondrial genotypes were found in the caspian sea among 35% of russian sturgeon specimens, but were not found in persian sturgeons. this confirms genetic isolation ...201628003882
[polymorphism of the myogens locus in some representatives of sturgeons (acipenseridae)].this study investigates muscle proteins in russian (acipenser gueldensiaedtii brandt), siberian (acipenser baerii brandt), and amur (acipenser shrencki brandt) sturgeons using the method of polyacrylamide gel electrophoresis. a group of fractions, which is presumably the product of a polyallelic duplicated my-1* locus, is recorded among the general pool of proteins. data from densitometric analysis makes it possible to put forward a hypothesis about an eight-gene determination of this system of ...201425735140
vertebral development and ossification in the siberian sturgeon (acipenser baerii), with new insights on bone histology and ultrastructure of vertebral elements and scutes.in order to improve our knowledge on the vertebral development, structure and mineralization in acipenseriformes, we undertook a study in a growth series of reared siberian sturgeons (acipenser baerii) using in toto clear and stain specimens, histological and ultrastructural observations, x-ray micro-tomography, and solid state nmr analyses. scutes were also studied to compare the tissue structure and mineralization of endoskeletal and dermal skeletal elements. this study completes and clarifies ...201727860464
establishment condition and characterization of heart-derived cell culture in siberian sturgeon (acipenser baerii).this study was conducted to establish the efficient condition for stable derivation of heart-derived cell culture in siberian sturgeon (acipenser baerii). three factors including isolation methods, cell densities in initial seeding, and basal media were evaluated for the derivation of heart-derived cell culture. as the results, enzymatic isolation was more efficient than mechanical isolation in both cell retrieval and further culture. total 48 trials of culture employing low and middle cell dens ...201425052194
siberian sturgeon oocyte extract induces epigenetic modifications of porcine somatic cells and improves developmental competence of scnt embryos.somatic cell nuclear transfer (scnt) has generally demonstrated that a differentiated cell can convert into a undifferentiated or pluripotent state. in the scnt experiment, nuclear reprogramming is induced by exposure of introduced donor nuclei to the recipient cytoplasm of matured oocytes. however, because the efficiency of scnt still remains low, a combination of scnt technique with the ex-ovo method may improve the normal development of scnt embryos. here we hypothesized that treatment of som ...201425049951
fatty acid composition in tissues of the farmed siberian sturgeon (acipenser baerii).the fatty acid (fa) compositions of the diet and diverse tissues of the farmed siberian sturgeon (acipenser baerii) were analyzed in detail to assess their nutritional quality. twelve male fish were sampled for muscle, fat, liver, brain, gill, kidney and gonad and the tissue fa measured by gas-liquid chromatography. the fa profile of the diet diverged from the fa signatures of the tissues, where the sturgeons accumulated particular highly-unsaturated fa (hufa). they were probably derived from th ...201424767029
3-d structure, volume, and dna content of erythrocyte nuclei of polyploid fish.we have explored the potential relationship between ploidy level, dna content (pg dna nucleus(-1)), and dimensional characteristics, such as volume (μm(3)), surface area (μm(2)), and 3-d structure of erythrocyte nuclei in a series of fish ploidy level models using feulgen image analysis densitometry, flow cytometry, and confocal laser scanning microscopy. the species were diploid tench (tinca tinca) (2n), cuban gar (atractosteus tristoechus) (2n), triploid tench (3n), evolutionary tetraploid ste ...201424446105
inhibition of β-n-acetylglucosaminidase by acetamide affects sperm motility and fertilization success of rainbow trout (oncorhynchus mykiss) and siberian sturgeon (acipenser baerii).β-n-acetylglucosaminidase (β-nagase) is an enzyme found in the sperm acrosome of numerous animal species including fish. fish spermatozoa differ in their morphology including acrosome or acrosomeless aquasperm in chondrostean (e.g., sturgeon) and teleostean (e.g., rainbow trout). it has been shown that β-nagase exists with high activity in both eggs and sperm of these species. the present study shows the potency of β-nagase in fertilization. in rainbow trout, increase in sperm motility parameter ...201424433780
fertility of a spontaneous hexaploid male siberian sturgeon, acipenser baerii.evolution of sturgeons and paddlefishes (order acipenseriformes) is inherently connected with polyploidization events which resulted in differentiation of ploidy levels and chromosome numbers of present acipenseriform species. moreover, allopolyploidization as well as autopolyploidization seems to be an ongoing process in these fishes and individuals with abnormal ploidy levels were occasionally observed within sturgeon populations. here, we reported occurrence of siberian sturgeon (acipenser ba ...201424410899
isolation and characterization of an atypical siberian sturgeon herpesvirus strain in russia: novel north american acipenserid herpesvirus 2 strain in europe?siberian sturgeon herpesvirus (sbshv) was isolated in russia for the first time in 2006. nine sbshv isolates were recovered from different fish hatcheries producing the same cytopathic effect in cell cultures, the same clinical signs and mortality kinetics in virus-infected fish and the same virus neutralization pattern and shared identical nucleotide sequences. in 2011, a new isolate was recovered from juvenile sturgeon, which caused completely different cytopathic effect. that isolate was not ...201728239935
persistence of environmental dna in freshwater ecosystems.the precise knowledge of species distribution is a key step in conservation biology. however, species detection can be extremely difficult in many environments, specific life stages and in populations at very low density. the aim of this study was to improve the knowledge on dna persistence in water in order to confirm the presence of the focus species in freshwater ecosystems. aquatic vertebrates (fish: siberian sturgeon and amphibian: bullfrog tadpoles) were used as target species. in control ...201121858099
intersex gonad differentiation in cultured russian (acipenser gueldenstaedtii) and siberian (acipenser baerii) sturgeon.among sturgeons, the occurrence of individuals with gonads containing both testis and ovary components is considered pathological, and such fish are described as intersex individuals or intersexes. intersexes are observed in both wild and cultured populations of sturgeon, usually at low frequencies. in the present study, intersex russian (acipenser gueldenstaedtii) and siberian (acipenser baerii) sturgeons constituted 30% of the studied populations. macroscopically, intersex gonads were recogniz ...201424403549
molecular cytogenetics in artificial hybrid and highly polyploid sturgeons: an evolutionary story narrated by repetitive sequences.we applied comparative genomic hybridization (cgh) and genomic in situ hybridization (gish) to examine genomes of artificially produced sturgeon hybrids between sterlet, acipenser ruthenus female (∼120 chromosomes) or russian sturgeon, a. gueldenstaedtii female (∼240 chromosomes) and a spontaneous triploid siberian sturgeon a. baerii male (∼360 chromosomes), respectively. the ploidy levels of progenies were analyzed by karyotyping and flow cytometry. we found that the species-specific regions we ...201324051427
antioxidant response versus selenium accumulation in the liver and kidney of the siberian sturgeon (acipenser baeri).the aim of this study was to examine the effects of selenium on concentrations of metabolites and enzyme activities acting as antioxidant markers in liver and kidney of siberian sturgeon acipenser baeri. sturgeons were fed selenium cysteine for 30 and 60 d at 1.25, 5, 20mg sekg(-1). selenium level in the control feed was 0.32 mg kg(-1). se concentration was measured in liver, kidney and muscle of every specimen. sturgeon accumulated se in tissues with a clear dose-response relationship and the h ...201324034889
comparative protein profiles: potential molecular markers from spermatozoa of acipenseriformes (chondrostei, pisces).sturgeon and paddlefish (acipenseriformes), the source of roe consumed as caviar, are a unique and commercially valuable group of ancient fishes. in this study, comparative proteomics was used to analyze protein profiles of spermatozoa from five sturgeon species and one paddlefish: siberian sturgeon (acipenser baerii), sterlet (a. ruthenus), russian sturgeon (a. gueldenstaedtii), starry sturgeon (a. stellatus), beluga (huso huso), and mississippi paddlefish (polyodon spathula). protein profiles ...201020869341
[discrete phenotypical variability of electrophoretic specters of one of myogen systems in the acipenseridae family].analysis of electrophoregrams of water-soluble proteins of white skeletal muscle of 10 fish species belonging to three genera of the acipenseridae family has allowed revealing an earlier not described system that can serve as a biochemical marker. this protein system represents a set of five fractions (a, b, c, d, e). a part of the studied species is monomorphous for one of these fractions (sterlet, starred sturgeon, great sturgeon, and big shovelnose had phenotype a, barbel sturgeon--phenotype ...201020799609
viable gynogenetic offspring of siberian sturgeon acipenser baeri brandt (pisces, acipenseridae) obtained from in vitro ovulating eggs. 201620506842
identification of sturgeon igd bridges the evolutionary gap between elasmobranchs and teleosts.igd has been found in almost all jawed vertebrates, including cartilaginous and teleost fish. however, igd is missing in acipenseriformes, a branch that is evolutionarily positioned between elasmobranchs and teleost fish. here, by analyzing transcriptome data, we identified a transcriptionally active igd-encoding gene in the siberian sturgeon (acipenser baerii). phylogenetic analysis indicated that it is orthologous to mammalian igd and closely related to the igd of other fish. the lengths of st ...201424001581
[the use of microsatellite loci for identification of sturgeon species (acipenseridae) and hybrid forms].the genetic polymorphism often sturgeon species that inhabit the territory of the russian federation (russian sturgeon, siberian sturgeon, amur sturgeon, sakhalin sturgeon, persian sturgeon, ship sturgeon, starlet, stellate sturgeon, beluga, and kaluga) was examined at five microsatellite loci (afug41, afug51, an20, aoxd161, aoxd165) (in total, 3821 individuals). the examined loci were successfully amplified with the same primer set in all species tested and demonstrated a high level of variatio ...201325508908
[the use of microsatellite loci for identification of sturgeon species (acipenseridae) and hybrid forms].the genetic polymorphism often sturgeon species that inhabit the territory of the russian federation (russian sturgeon, siberian sturgeon, amur sturgeon, sakhalin sturgeon, persian sturgeon, ship sturgeon, starlet, stellate sturgeon, beluga, and kaluga) was examined at five microsatellite loci (afug41, afug51, an20, aoxd161, aoxd165) (in total, 3821 individuals). the examined loci were successfully amplified with the same primer set in all species tested and demonstrated a high level of variatio ...201325486777
distribution of somatostatin immunoreactive neurons and fibres in the central nervous system of a chondrostean, the siberian sturgeon (acipenser baeri).somatostatin (som) is a neuropeptide that is widely distributed in the central nervous system of vertebrates. two isoforms of somatostatin (ss1 and ss2) have been characterized in sturgeon and in situ hybridisation studies in the sturgeon brain have demonstrated that mrnas of the two somatostatin precursors (pss1 and pss2) are differentially expressed in neurons [trabucchi, m., tostivint, h., lihrmann, i., sollars, c., vallarino, m., dores, r.m., vaudry, h., 2002. polygenic expression of somatos ...200818400215
ultrastructural study on the fertilisation process in sturgeon (acipenser), function of acrosome and prevention of polyspermy.sturgeon gametes differ from other fish in that their spermatozoa possess acrosome with finger-like posterolateral projections, which undergo exocytosis and filament formation, whereas eggs possess numerous micropyles. the fertilisation process in acipenser baerii was investigated by fluorescence and electron microscopy. a suitable activation solution containing 2.5 mm cacl(2), 15 mm tris, ph 10 was found for detailed description of acrosomal reaction. the acrosome reaction includes the formatio ...201019410390
isolation and transplantation of sturgeon early-stage germ cells.we report, for the first time, a series of baseline techniques comprising isolation and transplantation of female and male early-stage germ cells in sturgeon to generate a germline chimera as a potential tool for surrogate reproduction and gene banking. cells were dissociated from testis, characterized by mostly spermatogonia, and from ovary, exclusively comprising oogonia and previtellogenic oocytes, of acipenser baerii, using 0.3% trypsin (2 hours, 23 °c) dissolved in pbs, isotonic with blood ...201525559841
[molecular identification and the features of genetic diversity in interspecific hybrids of amur sturgeon (acipenser schrenckii x a. baerii, a. baerii x a. schrenckii, a. schrenckii x a. ruthenus, and a. ruthenus x a. schrenckii) based on variability of multilocus rapd markers].the method of polymerase chain reaction with random primers (rapd pcr) was used to identify the progeny of the crosses between three sturgeon species, amur sturgeon (acipenser schrenckii brandt, 1869), siberian sturgeon (a. baerii brandt, 1869), and sterlet (a. ruthenus linnaeus, 1758). using ten primers, genetic variation in 70 yearlings, produced in seven individual crosses: acipenser schrenckii x a. schrenckii, a. baerii x a. baerii, a. ruthenus x a. ruthenus, a. schrenckii x a. baerii, a. ba ...200819137727
morphological structure and peripheral innervation of the lateral line system in the siberian sturgeon (acipenser baerii).light and scanning electron microscopy (sem) were used to study the epidermal lateral line system of the siberian sturgeon (acipenser baerii brandt, 1869). this system consists of mechanoreceptive neuromasts, ampullae and the electroreceptive organ. the neuromasts are located in 5 pairs of cephalic and 1 pair of trunk canals and superficially in the middle and posterior pit lines that lie dorsomedially along the top of the skull immediately adjacent to the otic ampullae field. both canal neuroma ...201222405451
use of electrosense in the feeding behavior of sturgeons.the electro-receptive lateral line system appeared early in the evolutionary history of fish. sturgeons, members of the primitive chondrostei group, are known for their electroreceptors (ampullae of lorenzini) on the head, which are thought to be sensitive to weak electric fields in aquatic environments and involved in feeding, mating and migration. here, we report the results of a set of behavioral and electrophysiological experiments designed to determine the function and characteristics of th ...201222405450
effects of dietary bovine lactoferrin on growth, physiological performance, iron metabolism and non-specific immune responses of siberian sturgeon acipenser baeri.the present experiment was carried out to investigate the effects of different levels of dietary lactoferrin (lf) on growth performance, physiological status, iron absorption and innate immune response of juvenile siberian sturgeon acipenser baeri. fish were fed with six different rations including 0, 100, 200, 400, 800 and 1600 mg lf kg(-1) diet for 8 weeks. at the end of the experiment, samples were collected for estimating the physiological and immunological parameters. dietary lf did not cha ...201222377421
immunohistochemical study of the distribution of calcium binding proteins in the brain of a chondrostean (acipenser baeri).chondrosteans represent an ancient lineage in ray-finned bony fishes and hence in jawed vertebrates. this immunohistochemical study in the brain of the siberian sturgeon reports the neuronal distribution of three cytosolic calcium-binding proteins: calbindin-d28k (cb), calretinin (cr), and parvalbumin (pv). cb and cr are widely expressed in different neuron subsets distributed throughout the sturgeon brain. studies using double immunofluorescence reveal a wide co-distribution of cb and cr in the ...201222173872
[impacts of ultraviolet irradiation on the sperm motility and longevity of acipenser baerii].this paper studied the impacts of different dose ultraviolet irradiation (254 nm, uvc) on the sperm motility and longevity of acipenser baerii. ultraviolet irradiation had significant impacts on the sperm motility, its fast motion time, and longevity. with the increasing dose of ultraviolet irradiation, the sperm motility decreased rapidly first, increased rapidly then, and decreased rapidly again. the sperm fast motion time had the similar variation trend as the sperm motility, but the sperm lo ...201122097385
anatomical relations between neuropeptide y, galanin, and gonadotropin-releasing hormone in the brain of chondrostean, the siberian sturgeon acipenser baeri.we investigated the immunohistochemical localization of immunoreactive (ir) cell bodies and fibers of neuropeptide y (npy) and galanin (gal), and the anatomical relations between these neurons in the brain of the siberian sturgeon acipenser baeri to clarify the interactions between these neuropeptides. furthermore, the anatomic relations between npy and gonadotropin-releasing hormone (gnrh) in the brain were also examined. npy-ir cell bodies were observed in the ventral part of the ventral telen ...201121871949
[dispermic androgenesis in sturgeons with the help of cryopreserved sperm: production of androgenetic hybrids between siberian and russian sturgeons].dispermic androgenesis was used to produce, for the first time, an androgenetic progeny of the siberian sturgeon (acipenser baerii) and the androgenetic nuclear cytoplasmic hybrids (siberian sturgeon, a. baerii x russian sturgeon, a. gueldenstaedtii) using cryopreserved sperm. microsatellite dna analysis confirmed exclusively paternal inheritance in the androgenetic progeny of siberian sturgeon. heterozygotes for certain microsatellite loci were detected among the androgenetic hybrids, thereby c ...201121542342
distribution of glycine immunoreactivity in the brain of the siberian sturgeon (acipenser baeri): comparison with γ-aminobutyric acid.glycine and γ-aminobutyric acid (gaba) are the main inhibitory neurotransmitters in the central nervous system (cns) of vertebrates. studies on the distribution of glycinergic neurons and fibers have been carried out mainly in rodents and lampreys. with the aim of discovering more about the early evolution of this system in vertebrates, we analyzed the distribution of glycine-immunoreactive (gly-ir) neurons and fibers in the cns of a basal ray-finned fish, the siberian sturgeon (chondrostei, aci ...201121344405
magnitude of the root effect in red blood cells and haemoglobin solutions of fishes: a tribute to august krogh.the ability of high carbon dioxide tensions or low ph to reduce blood oxygen binding even at high oxygen tensions, first observed by august krogh and isabella leitch in 1919 and now known as the root effect, was studied in red blood cells and haemoglobin solutions of several fish species.201121199396
[effects of storage medium and temperature on short-term storage of acipenser baerii eggs].this paper studied the effects of different storage medium (coelomic fluid, hepes solution, and modified ringer's solution), temperature (4 degrees c and 16 degrees c), and duration (4 h, 8 h, 16 h, and 24 h) on the short-term storage of siberian sturgeon (acipenser baerii) eggs. the results showed that storage medium, temperature, and duration all had significant effects on the fertilization rate, hatching success, and malformation of newly hatched larvae (p < 0.05). with the increase of storag ...201020387448
testing taxonomic boundaries and the limit of dna barcoding in the siberian sturgeon, acipenser baerii.dna barcoding efforts involving animals have focused on the mitochondrial cytochrome c oxidase subunit i (cox1) gene. some authors suggest that this marker might under-diagnose young species. herein, we examine cox1 and control region diversity in a sample of siberian sturgeon (acipenser baerii), a species with an extremely wide geographic distribution in the major rivers of siberia and in lake baikal. some authors currently recognize three subspecies within this species. these subspecies are re ...200919900060
[anomalies in the development and functioning of the reproductive system in siberian sturgeon, acipenser baerii brandt (acipenseridae), from the yenisei river].based upon long-term studies, the alterations in the development and functioning of the reproductive system in the yenisei river siberian sturgeon are analyzed. primary attention is paid to the malfunctions of gametogenesis during trophoplasmatic growth of the oocytes. some anomalies were revealed for the first time in the studied population. however, these anomalies are typical for other populations of this species. these anomalies are not species-specific and may be found in other representati ...201619894605
[effects of initial feeding on the growth, survival, and body biochemical composition of siberian sturgeon (acipenser baerii) larvae].siberian sturgeon (acipenser baerii) larvae were raised with limnodrilus sp., artemia nauplii, moina sp., and artificial commercial diet for 30 days to investigate the effects of different initial feeding diets on the growth, survival, and body biochemical composition of the larvae. the results showed that a. nauplii was the best initial feeding diet, with the survival rate of a. baerii larvae being significant higher (96.67%) than the others. feeding with limnodrilus sp. gave the highest growth ...200919459376
glycine-immunoreactive neurons in the brain of a shark (scyliorhinus canicula l.).the glycinergic cell populations in the brain of the lesser spotted dogfish were studied by a glycine immunofluorescence method. numerous glycine-immunoreactive (gly-ir) neurons were observed in different brain nuclei. in the telencephalon, gly-ir cells were observed in the olfactory bulb, telencephalic hemispheres, and preoptic region. in the hypothalamus, cerebrospinal fluid-contacting gly-ir neurons were observed in the lateral and posterior recess nuclei. coronet cells of the saccus vasculos ...201323630026
effect of short-term storage on quality parameters, dna integrity, and oxidative stress in russian (acipenser gueldenstaedtii) and siberian (acipenser baerii) sturgeon sperm.the sperm of russian sturgeon (a. gueldenstaedtii) and siberian sturgeon (a. baerii) was used to evaluate the effects of short-term (liquid) storage on functional parameters (spermatozoa motility and velocity), dna integrity and oxidative stress indices. spermatozoa showed >50% motility during 6 days of storage with an average velocity of 133.12±15.4 to 87.9±11.23μm s(-1) in both species. no motile spermatozoa were recorded after nine days of storage. analysis of russian sturgeon sperm showed no ...201323602487
non-contact doppler radar monitoring of cardiorespiratory motion for siberian sturgeon.this paper presents the first reported use of doppler radar to remotely sense heart and ventilation rates of fish. the radar reported 35 to 40 bpm heart rate and 115 to 145 bpm ventilation rates for siberian sturgeon, with agreement from a video reference. conventional fish vital signs measurements require invasive surgery and human handling--these are problematic for large scale monitoring, for measuring deep sea fish, and other situations which preclude human interaction with each individual s ...201223366402
fins improve the swimming performance of fish sperm: a hydrodynamic analysis of the siberian sturgeon acipenser baerii.the flagella of sturgeon sperm have an ultrastructure comprising paddle-like fins extending along most of their length. these fins are seen in several other marine and freshwater fish. the sperm of these fish are fast swimmers and are relatively short lived: it is therefore tempting to think of these fins as having evolved for hydrodynamic advantage, but the actual advantage they impart, at such a small length scale and slow speed, is unclear. the phrase "the fins improve hydrodynamic efficiency ...201323233331
effects of arabinoxylan-oligosaccharides (axos) on juvenile siberian sturgeon (acipenser baerii) performance, immune responses and gastrointestinal microbial community.arabinoxylan-oligosaccharides (axos) are a newly discovered class of candidate prebiotics that exert different properties depending on their structure. in this study the effects of two different structures of axos, namely axos-32-0.30 (average degree of polymerization: 32, average degree of substitution: 0.30) and axos-3-0.25, were investigated on growth performance, immune responses, gut microbial fermentation and gut bacterial composition of juvenile siberian sturgeon (acipenser baerii). after ...201222796425
expression of dmrt1 and sox9 during gonadal development in the siberian sturgeon (acipenser baerii).the sex differentiation period of the siberian sturgeon was investigated through expression profiling of two testicular markers (dmrt1 and sox9). at the molecular level, a clear sexual dimorphism of dmrt1 and sox9 was observed in 3-year-old fish with immature gonads, in which males showed higher expression of these genes. among 16-month-old sturgeons cultured in uruguay, gonad morphology analyses showed one group of fish with undifferentiated gonads and a second group which had started their his ...201322688449
[development of the lateral line system in juvenile siberian sturgeon (acipenser baerii)].the siberian sturgeon (acipenser baerii brandt), a chondrostean, occupies an important position in the evolution of the electroreceptor. in order to more fully understanding the evolution of these receptors, we examined the development of the lateral line system during early ontogeny of the siberian sturgeon by using light and scanning electron microscopes. we detected four major events in this process: the lateral line placodal development, the sensory ridge formation, the receptor formation an ...201222653853
the bioaccumulation of fluoride ion (f(-)) in siberian sturgeon (acipenser baerii) under laboratory conditions.a 90-d growth trial was conducted to determine the accumulation of fluoride ions (f(-)) in juvenile siberian sturgeon (acipenser baerii). fish were exposed to nominal f(-) concentrations of 4, 10, 25, 62.5 mg l(-1) (added as naf), along with a control group. fish growth was determined and f(-) concentrations in bone, cartilage, skin, gill, muscle, liver, gut and pylorus were analyzed by fluoride specific electrode method. fish growth was inhibited for groups exposed to 10, 25, and 62.5 mg f(-) l ...200919150727
expression and phylogeny of candidate genes for sex differentiation in a primitive fish species, the siberian sturgeon, acipenser baerii.the molecular mechanisms underlying testis differentiation in basal actinopterygian fish remains poorly understood. the sex differentiation period was investigated in the siberian sturgeon, acipenser baerii, by expression profiling of sertoli cell transcription factors (dmrt1, sox9) that control testis differentiation in vertebrates; leydig cell factors (cyp17a1, star) affecting androgen production; the androgen receptor (ar); a growth factor controlling testis development (igf1); and a gene cod ...201222618959
[cloning and expression of tbx3 gene in siberian sturgeon, acipenser baerii].tbx3, a member of the tbx2 subfamily of t-box gene family, encodes a transcription factor with a highly conserved dna-binding domain, which called t-domain. tbx3 is involved in morphogenesis and organogenesis in vertebrates, such as limb development, heart remodeling, and neural placode differentiation. in the present study, a full-length 2 908 bp tbx3 cdna from acipenser baerii (abtbx3) was obtained using rt-pcr and race technique, which includes a 2 166 bp complete open reading frame encoding ...201222467390
mitochondrial genome of the siberian sturgeon acipenser baerii.the complete mitochondrial genome of siberian sturgeon acipenser baerii had been determined. the total length of the mitogenome was 16,763 bp and had 13 protein-coding, 22 trna, and 2 rrna genes. except for the eight trna and nd6 genes, all other mitochondrial genes are encoded on the heavy strand. the overall base composition of the heavy strand is 30.3% a, 24.3% t, 29.1% c, 16.3% g, with an at content of 54.6%. the dna sequence of a. baerii shared 97.8, 97.6, 93.5, 91.6, 93.6, and 87.1% sequen ...201222409751
long-term culture of a cell population from siberian sturgeon (acipenser baerii) head kidney.in vitro cultures of native fish cell lines are of great importance, both for basic research and applied science. in particular, there is strong demand for long-term growable cell lines from breeding fish, like sturgeon. here, we describe the culture of cells from siberian sturgeon (acipenser baerii) head kidney. the cells have so far been cultured over a period of 12 months (24 passages). cytochemical and immunocytochemical examination suggests that, in vitro, the cells exhibit markers that are ...200818958594
[polymorphism of the mitochondrial dna control region in eight sturgeon species and development of a system for dna-based species identification].intraspecific and interspecific nucleotide sequence variations of the mtdna control region (d-loop) were studied with mtdnas isolated from tissue specimens of more than 1400 sturgeons of nine species: russian sturgeon acipenser gueldenstaedtii, persian sturgeon a. persicus, siberian sturgeon a. baerii, amur sturgeon a. schrenkii, fringebarbel sturgeon a. nudiventris, sterlet a. ruthenus, stellate sturgeon a. stellatus, beluga huso huso, and kaluga h. dauricus. the results were used to analyze th ...200818767539
diverse splicing pathways of the membrane ighm pre-mrna in a chondrostean, the siberian sturgeon.teleosts and tetrapods have evolved different splice patterns to generate their membrane-bound igm. in the tetrapod lineage, the first transmembrane exon is spliced to an internal cryptic site located close to the end of the fourth constant exon. because teleosts lack this site they use the regular 3'-splice site of the ch3 exon instead. we characterized the mum splicing patterns in a chondrostean, the siberian sturgeon. we observed a surprising diversity of splice patterns, the tm1 exon being s ...200919027787
molecular genetic analysis among subspecies of two eurasian sturgeon species, acipenser baerii and a. stellatus.two species, the siberian sturgeon, acipenser baerii, and stellate sturgeon, a. stellatus, were studied using mitochondrial dna (mtdna) (d-loop, cytochrome b (cyt-b) and nd5/6 genes) sequencing to determine whether traditionally defined subspecies correspond to taxonomic entities and conservation management units. initially, several mtdna regions for each taxon (a. baerii: 737 bp d-loop, 750 bp nd5, 200 bp nd6, and 790 bp cyt-b; a. stellatus: 737 bp d-loop and 600 bp nd5) were examined. the d-lo ...199910703555
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