attraction of the oriental fruit fly, dacus dorsalis, to methyl eugenol and related olfactory stimulants. | the attraction of male oriental fruit flies to methyl eugenol and 34 analogues was investigated quantitatively using the characteristic feeding response. methyl eugenol was the most active compound studied, with a feeding response to 0.01 mug, but saturation of the allyl side chain or replacement of allyl by allyloxy produced compounds almost as effective. replacement of the methoxy groups by methylenedioxy, methyl, or chloro groups abolished all response. the ring geometry of the methoxy groups ... | 1975 | 1058469 |
saturated and monofluoro analogs of the oriental fruit fly attractant methyl eugenol show reduced genotoxic activities in yeast. | methyl eugenol, is a commercially used fruit fly attractant and a suspected carcinogen. several phenylpropenes, including methyl eugenol and the known carcinogen safrole, score negative in the salmonella assay but score positive in the yeast del assay that selects for intrachromosomal recombination events in the yeast saccharomyces cerevisiae. in an attempt to dissociate the beneficial properties of methyl eugenol from its genotoxic properties, saturated or fluorinated analogs were evaluated for ... | 1996 | 8792836 |
the hobo transposable element excises and has related elements in tephritid species. | function of the drosophila melanogaster hobo transposon in tephritid species was tested in transient embryonic excision assays. wild-type and mutant strains of anastrepha suspensa, bactrocera dorsalis, b. cucurbitae, ceratitis capitata, and toxotrypana curvicauda all supported hobo excision or deletion both in the presence and absence of co-injected hobo transposase, indicating a permissive state for hobo mobility and the existence of endogenous systems capable of mobilizing hobo. in several str ... | 1996 | 8807305 |
field infestation of rambutan fruits by internal-feeding pests in hawaii. | more than 47,000 mature fruits of nine different varieties of rambutan (nephelium lappaceum l.) were harvested from orchards in hawaii to assess natural levels of infestation by tephritid fruit flies and other internal feeding pests. additionally, harvested, mature fruits of seven different rambutan varieties were artificially infested with eggs or first-instars of mediterranean fruit fly, ceratitis capitata (wiedemann), or oriental fruit fly, bactrocera dorsalis (hendel) (diptera: tephritidae) ... | 2000 | 10902340 |
use of the piggybac transposon for germ-line transformation of insects. | germ-line transformation of insects is now possible with four independent transposable element vector systems. among these, the ttaa-insertion site specific transposon, piggybac, discovered in trichoplusia ni, is one of the most widely used. transformations have been achieved in a wide variety of dipterans, lepidopterans, and a coleopteran, and for many species, piggybac transposition was first tested by plasmid-based mobility assays in cell lines and embryos. all plasmid and genomic insertions ... | 2002 | 12225912 |
molecular phylogeny of tephritid fruit flies in the bactrocera tau complex using the mitochondrial coi sequences. | we compared sequences of the mitochondrial cytochrome oxidase i gene of eight species of the bactrocera tau complex using bactrocera dorsalis, bactrocera pyrifoliae, ceratitis capitata, anopheles gambiae, and locusta migratoria as outgroups. a 639-bp variable region was sequenced. the sequence divergence between species in the b. tau complex ranged from 0.06 to 28%, and up to 29% between the complex and its tephritid outgroups, b. dorsalis and c. capitata. according to the phylogenetic relations ... | 2003 | 12669803 |
isolation and analysis of a new hopper hat transposon from the bactrocera dorsalis white eye strain. | a new hopper element belonging to the hat transposon family was isolated from the white eye mutant strain of the oriental fruit fly, bactrocera dorsalis. using the original hopper element sequence from the wild type kahuku strain as a template, the new hopper was isolated by inverse and direct pcr. nucleotide sequence analysis reveals a 3131 bp element with terminal and subterminal inverted repeat sequences, an 8 bp duplicated insertion site, and a conceptual translation yielding a single uninte ... | 2003 | 12733661 |
molecular evolutionary analysis of the widespread piggybac transposon family and related "domesticated" sequences. | piggybac is a short inverted-repeat-type dna transposable element originally isolated from the genome of the moth trichoplusia ni. it is currently the gene vector of choice for the transformation of various insect species. a few sequences with similarity to piggybac have previously been identified from organisms such as humans ( looper), the pufferfish takifugu rubripes ( pigibaku), xenopus ( tx), daphnia ( pokey), and the oriental fruit fly bactrocera dorsalis. we have now identified 50 piggyba ... | 2003 | 12955498 |
an active transposable element, herves, from the african malaria mosquito anopheles gambiae. | transposable elements have proven to be invaluable tools for genetically manipulating a wide variety of plants, animals, and microbes. some have suggested that they could be used to spread desirable genes, such as refractoriness to plasmodium infection, through target populations of anopheles gambiae, thereby disabling the mosquito's ability to transmit malaria. to achieve this, a transposon must remain mobile and intact after the initial introduction into the genome. endogenous, active class ii ... | 2005 | 15545643 |
[population dynamics of bactrocera dorsalis (diptera: tephritidae) in xishuangbanna of southern yunnan]. | in the study, an all year round monitoring through lure-traps in 1997, 2000 and 2003 was conducted on the population dynamics of oriental fruit fly, bactrocera dorsalis (diptera: tephritidae) in xishuangbanna of southern yunnan, and the factors in relation with its population fluctuation, including temperature, rainfall, and host species were analyzed. the fruit fly occurred year-round in xishuangbanna, with a low population level from november to february, and increased steadily from march to j ... | 2005 | 16252878 |
highly conserved piggybac elements in noctuid species of lepidoptera. | the piggybac transposable element was originally discovered in a trichoplusia ni cell line and nearly identical elements were subsequently discovered in the tephritid fly, bactrocera dorsalis. this suggested the existence of piggybac in additional insects and this study shows highly conserved, though not identical, piggybac sequences in the noctuid species heliocoverpa armigera, h. zea, and spodoptera frugiperda, as well as new piggybac sequences from the t. ni organismal genome. genomic piggyba ... | 2006 | 16651189 |
effectiveness of protein baits on melon fly and oriental fruit fly (diptera: tephritidae): attraction and feeding. | attraction and feeding responses of oriental fruit fly, bactrocera dorsalis (hendel), and melon fly, bactrocera cucurbitae (coquillett), were determined for different protein baits. in separate choice attraction assays for each species, significantly more flies arrived at stations with bait than water, but no differences existed among baits of gf-120 fruit fly bait, gf-120 nf naturalyte fruit fly bait, provesta 621 autolyzed yeast extract, and mazoferm e802. in comparison with b. dorsalis, b. cu ... | 2006 | 16937668 |
female-biased attraction of oriental fruit fly, bactrocera dorsalis (hendel), to a blend of host fruit volatiles from terminalia catappa l. | coupled gas chromatography-electroantennogram detection (gc-ead) analysis of volatiles from tropical almond fruit, terminalia catappa l., revealed 22 compounds that were detected by antennae of oriental fruit fly females, bactrocera dorsalis (hendel). both solid-phase microextraction (spme) and porapak q were used for sampling odors in fruit headspace, with spme collections producing larger ead responses from a greater number of compounds. geranyl acetate and methyl eugenol elicited the largest ... | 2006 | 17082987 |
attraction of bactrocera dorsalis (diptera: tephritidae) and nontarget insects to methyl eugenol bucket traps with different preservative fluids on oahu island, hawaiian islands. | attraction of oriental fruit fly, bactrocera dorsalis (hendel) (diptera: tephritidae), and nontarget insects to preservative fluids ethylene glycol antifreeze, propylene glycol antifreeze, or mineral oil in bucket traps that contained captured decaying male oriental fruit flies, a male lure (methyl eugenol), and a toxicant (ddvp vapor insecticidal strip) were compared with dry control traps. significantly (p < 0.05) greater numbers of b. dorsalis were captured in propylene glycol antifreeze trap ... | 2007 | 17598531 |
diversity and phylogeny of wolbachia infecting bactrocera dorsalis (diptera: tephritidae) populations from china. | wolbachia are a common and widespread group of symbiotic bacteria found in the reproductive tissues of arthropods. bactrocera dorsalis (hendel) is an important pest causing considerable economic losses of fruits and vegetables in several southern provinces of china. in this study, polymerase chain reaction (pcr) with general wolbachia surface protein (wsp) primers was used to test the presence of wolbachia in 1,500 individuals of b. dorsalis from five geographical populations of china. we detect ... | 2007 | 18284754 |
evaluation of lure dispensers for fruit fly surveillance in new zealand. | fruit flies (diptera: tephritidae) represent a major biosecurity threat to the horticulture sector of new zealand, which is entirely free of these invasive pests. a nationwide surveillance programme is conducted to ensure any incursion is detected as early as possible. a review of the lure dispensers used is reported here. | 2008 | 18383565 |
thermal responses of oriental fruit fly (diptera: tephritidae) late third instars: mortality, puparial morphology, and adult emergence. | responses of late third instars of the oriental fruit fly, bactrocera dorsalis (hendel) (diptera: tephritidae), to high temperatures (43, 46, and 48 degrees c) were investigated. the different heat exposures not only affected the timing of death but also induced different quantities of malformed puparia and changed the average eclosion time. a majority of larvae died immediately (as larvae) after 30 min at 46 degrees c and > or =15 min at 48 degrees c, whereas most individuals died as pupae afte ... | 2008 | 18613573 |
captures in methyl eugenol and cue-lure detection traps with and without insecticides and with a farma tech solid lure and insecticide dispenser. | methyl eugenol (me) and cue-lure (c-l) traps to detect tephritid flies on the u.s. mainland were tested with and without insecticides under hawaiian weather conditions against small populations of oriental fruit fly, bactrocera dorsalis (hendel) and melon fly, bactrocera cucurbitae (coquillett), respectively. in comparative tests, standard jackson traps with naled and the hawaii fruit fly areawide pest management (awpm) trap with 2,2-dichorovinyl dimethyl phosphate (ddvp) insecticidal strips out ... | 2009 | 19449634 |
bactrocerin-1: a novel inducible antimicrobial peptide from pupae of oriental fruit fly bactrocera dorsalis hendel. | a novel antimicrobial peptide, bactrocerin-1, was purified and characterized from an immunized dipteran insect, bactrocera dorsalis. bactrocerin-1 has 20 amino acid residues with a mass of 2,325.95 da. the amino acid sequence of bactrocerin-1 showed very high similarity to the active fragment (46v-65s-nh(2)) of coleoptericin a. the composition of amino acid residues revealed that bactrocerin-1 is a hydrophobic, positively charged, and lys/ile/gly-rich peptide. minimal growth inhibition concentra ... | 2009 | 19479741 |
the beta2-tubulin gene from three tephritid fruit fly species and use of its promoter for sperm marking. | to isolate testis-specific regulatory dna that could be used in genetically transformed insect pest species to improve their biological control, beta2-tubulin genes and their proximal genomic dna were isolated from three economically important tephritid pest species, anastrepha suspensa, anastrepha ludens, and bactrocera dorsalis. gene isolation was first attempted by degenerate pcr on an a. suspensa adult male testes cdna library, which fortuitously isolated the 2.85 kb beta1-tubulin gene that ... | 2009 | 19520163 |
puncture resistance in 'sharwil' avocado to oriental fruit fly and mediterranean fruit fly (diptera: tephritidae) oviposition. | the physiological basis for host antibiosis or nonpreference to a quarantine pest is often not understood. studies are needed on the mechanisms that impart resistance to better understand how resistance might fail. experiments were conducted to examine the infestability of 'sharwil' avocados by oriental fruit fly, bactrocera dorsalis (hendel), and mediterranean fruit fly, ceratitis capitata (wiedemann) (diptera: tephritidae), after harvest and to quantify the effect of avocado skin hardness on r ... | 2009 | 19610403 |
evaluation of yeasts and yeast products in larval and adult diets for the oriental fruit fly, bactrocera dorsalis, and adult diets for the medfly, ceratitis capitata, and the melon fly, bactrocera curcurbitae. | several yeasts and yeast products were tested in adult diets for the medfly ceratitis capitata (wiedemann), oriental fruit fly bactrocera dorsalis (hendel), and melon fly, bactrocera curcurbitae (coquillett) (diptera: tephritidae) and in larval liquid diet for mass-rearing b. dorsalis. three hydrolyzed brewer's yeasts (fnils65, fni200 and fni210), one glutamine enriched yeast (gsh), one vitamin-enriched yeast (rda500), korea yeast, whole cell yeasts, and combinations of them were evaluated. adul ... | 2009 | 19613830 |
population genetic structure of the oriental fruit fly, bactrocera dorsalis (hendel) (diptera: tephritidae) from yunnan province (china) and nearby sites across the border. | bactrocera dorsalis (diptera: tephritidae) is an important pest for many tropical and subtropical fruits. the fly is probably introduced in yunnan, a southwestern province of china that shares borders with vietnam, laos and myanmar. depending on local environmental conditions, this species occurs either only in the most favorable seasons or year-round. to infer the genetic diversity and structure of the fly in the region, and to understand the relationships between the flies of year-round and se ... | 2010 | 20012674 |
comparison of the diversity of the bacterial communities in the intestinal tract of adult bactrocera dorsalis from three different populations. | to (i) identify the bacterial communities in the gut of oriental fruit fly (bactrocera dorsalis) adult and (ii) determine whether the different surroundings and diets influence the bacteria composition. | 2011 | 21395953 |
rna interference of four genes in adult bactrocera dorsalis by feeding their dsrnas. | rna interference (rnai) is a powerful method to inhibit gene expression in a sequence specific manner. recently silencing the target gene through feeding has been successfully carried out in many insect species. | 2011 | 21445257 |
a compound produced by fruigivorous tephritidae (diptera) larvae promotes oviposition behavior by the biological control agent diachasmimorpha longicaudata (hymenoptera: braconidae). | tephritid fruit fly parasitoids use fruit-derived chemical cues and the vibrations that result from larval movements to locate hosts sequestered inside fruit. however, compounds produced by the larvae themselves have not been previously described nor their significance to parasitoid foraging determined. we collected the volatiles from four species of tropical and subtropical tephritidae: anastrepha suspensa (loew), bactrocera dorsalis hendel, bactrocera cucurbitae coquillett, and ceratitis capit ... | 2011 | 22251652 |
morphological features of the ovaries during oogenesis of the oriental fruit fly, bactrocera dorsalis, in relation to the physiological state. | determination of physiological state in insects is useful in furthering the understanding of how insect behavior changes with age. central to this determination is the identification of characters that allow assessment of physiological age. while non-destructive measures produce the most desired outcomes, internal markers may be more diagnostic and reliable. in this study, key morphological characters during previtellogenesis through vitellogenesis and ovulation were assessed as markers to deter ... | 2012 | 23463982 |
salicylic acid induces changes in mango fruit that affect oviposition behavior and development of the oriental fruit fly, bactrocera dorsalis. | the oriental fruit fly, bactrocera dorsalis (hendel) is an important quarantine pest around the globe. although measures for its control are implemented worldwide through ipm and male annihilation, there is little effect on their population. hence, there is a need for new strategies to control this minacious pest. a strategy that has received negligible attention is the induction of 'natural plant defenses' by phytohormones. in this study, we investigated the effect of salicylic acid (sa) treatm ... | 2015 | 26422203 |
characterization of bactrocera dorsalis serine proteases and evidence for their indirect role in insecticide tolerance. | the oriental fruit fly bactrocera dorsalis (hendel) causes devastating losses to agricultural crops world-wide and is considered to be an economically important pest. little is known about the digestive enzymes such as serine proteases (sps) in b. dorsalis, which are important both for energy supply and mitigation of fitness cost associated with insecticide tolerance. in this study, we identified five sp genes in the midgut of b. dorsalis, and the alignments of their deduced amino acid sequences ... | 2014 | 24566149 |
identification of male- and female-specific olfaction genes in antennae of the oriental fruit fly (bactrocera dorsalis). | the oriental fruit fly (bactrocera dorsalis) is a species of tephritid fruit fly, endemic to southeast asia but also introduced to many regions of the us, and it is one of the major pest species with a broad host range of cultivated and wild fruits. although males of b. dorsalis respond strongly to methyl eugenol and this is used for monitoring and estimating populations, the molecular mechanism of the oriental fruit fly olfaction has not been elucidated yet. therefore, in this project, using ne ... | 2016 | 26845547 |
functional characterization of nadph-cytochrome p450 reductase from bactrocera dorsalis: possible involvement in susceptibility to malathion. | nadph cytochrome p450 reductase (cpr) is essential for cytochrome p450 catalysis, which is important in the detoxification and activation of xenobiotics. in this study, two transcripts of bactrocera dorsalis cpr (bdcpr) were cloned, and the deduced amino-acid sequence had an n-terminus membrane anchor for bdcpr-x1 and three conserved binding domains (fmn, fad, and nadp), as well as an fad binding motif and catalytic residues for both bdcpr-x1 and bdcpr-x2. bdcpr-x1 was detected to have the high ... | 2015 | 26681597 |
flight capacity of bactrocera dorsalis (diptera: tephritidae) adult females based on flight mill studies and flight muscle ultrastructure. | the oriental fruit fly, bactrocera dorsalis (hendel) (diptera: tephritidae), is considered a major economic threat in many regions worldwide. to better comprehend flight capacity of b. dorsalis and its physiological basis, a computer-monitored flight mill was used to study flight capacity of b. dorsalis adult females of various ages, and the changes of its flight muscle ultrastructures were studied by transmission electron microscopy. the flight capacity (both speed and distance) changed signifi ... | 2015 | 26450591 |
identification and expression profile analysis of odorant binding proteins in the oriental fruit fly bactrocera dorsalis. | olfaction is crucial in many insects for critical behaviors, including those regulating survival and reproduction. insect odorant-binding proteins (obps) function in the first step of the olfactory system and play an essential role in the perception of odorants, such as pheromones and host chemicals. the oriental fruit fly, bactrocera dorsalis, is a destructive fruit-eating pest, due to its wide host range of up to 250 different types of fruits and vegetables, and this fly causes severe economic ... | 2013 | 23867609 |
molecular characteristics, mrna expression, and alternative splicing of a ryanodine receptor gene in the oriental fruit fly, bactrocera dorsalis (hendel). | ryanodine receptors (ryrs) are a distinct class of ligand-gated channels controlling the release of calcium from intracellular stores. the emergence of diamide insecticides, which selectively target insect ryrs, has promoted the study of insect ryrs. in the present study, the full-length ryr cdna (bdryr) was cloned and characterized from the oriental fruit fly, bactrocera dorsalis (hendel), a serious pest of fruits and vegetables throughout east asia and the pacific rim. the cdna of bdryr contai ... | 2014 | 24740254 |
de novo cloning and annotation of genes associated with immunity, detoxification and energy metabolism from the fat body of the oriental fruit fly, bactrocera dorsalis. | the oriental fruit fly, bactrocera dorsalis, is a destructive pest in tropical and subtropical areas. in this study, we performed transcriptome-wide analysis of the fat body of b. dorsalis and obtained more than 59 million sequencing reads, which were assembled into 27,787 unigenes with an average length of 591 bp. among them, 17,442 (62.8%) unigenes matched known proteins in the ncbi database. the assembled sequences were further annotated with gene ontology, cluster of orthologous group terms, ... | 2014 | 24710118 |
computational reverse chemical ecology: virtual screening and predicting behaviorally active semiochemicals for bactrocera dorsalis. | semiochemical is a generic term used for a chemical substance that influences the behaviour of an organism. it is a common term used in the field of chemical ecology to encompass pheromones, allomones, kairomones, attractants and repellents. insects have mastered the art of using semiochemicals as communication signals and rely on them to find mates, host or habitat. this dependency of insects on semiochemicals has allowed chemical ecologists to develop environment friendly pest management strat ... | 2014 | 24640964 |
discovery of genes related to insecticide resistance in bactrocera dorsalis by functional genomic analysis of a de novo assembled transcriptome. | insecticide resistance has recently become a critical concern for control of many insect pest species. genome sequencing and global quantization of gene expression through analysis of the transcriptome can provide useful information relevant to this challenging problem. the oriental fruit fly, bactrocera dorsalis, is one of the world's most destructive agricultural pests, and recently it has been used as a target for studies of genetic mechanisms related to insecticide resistance. however, prior ... | 2012 | 22879883 |
rna sequencing to characterize transcriptional changes of sexual maturation and mating in the female oriental fruit fly bactrocera dorsalis. | female reproductive potential plays a significant role in the survival and stability of species, and sexual maturation and mating processes are crucial. however, our knowledge of the reproductive genes involved in sexual maturation and mating has been largely limited to model organisms. the oriental fruit fly bactrocera dorsalis is a highly invasive agricultural pest, known to cause major economic losses; thus, it is of great value to understand the transcriptional changes involved in sexual mat ... | 2016 | 26946038 |
identification, mrna expression, and functional analysis of chitin synthase 1 gene and its two alternative splicing variants in oriental fruit fly, bactrocera dorsalis. | two alternative splicing variants of chitin synthase 1 gene (bdchs1) were cloned and characterized from the oriental fruit fly, bactrocera dorsalis (hendel). the cdna of both variants (bdchs1a and bdchs1b) consisted of 5,552 nucleotides (nt), with an open reading frame (orf) of 4,776 nt, encoding a protein of 1,592 amino acid residues, plus 685- and 88-nt of 5'- and 3'-noncoding regions, respectively. the alternative splicing site was located between positions 3,784-3,960 and formed a pair of mu ... | 2013 | 23569438 |
the essential role of vitellogenin receptor in ovary development and vitellogenin uptake in bactrocera dorsalis (hendel). | the vitellogenin receptor (vgr) functions as an essential component in uptaking and transporting vitellogenin (vg) in female adults, which is involved in ovary development and oviposition. this study aimed to clarify the molecular characteristics and function of vgr in the oriental fruit fly bactrocera dorsalis (hendel). here, we identified the full-length of bdvgr (genbank accession no. jx469118), encoding a 1925 residue (aa) protein with a 214.72 kda molecular mass and several typical motifs o ... | 2015 | 26262609 |
high-throughput sequencing to reveal genes involved in reproduction and development in bactrocera dorsalis (diptera: tephritidae). | tephritid fruit flies in the genus bactrocera are of major economic significance in agriculture causing considerable loss to the fruit and vegetable industry. currently, there is no ideal control program. molecular means is an effective method for pest control at present, but genomic or transcriptomic data for members of this genus remains limited. to facilitate molecular research into reproduction and development mechanisms, and finally effective control on these pests, an extensive transcripto ... | 2012 | 22570719 |
transcriptome analysis of the oriental fruit fly (bactrocera dorsalis). | the oriental fruit fly, bactrocera dorsalis (hendel), is one of the most economically important pests in the world, causing serious damage to fruit production. however, lack of genetic information on this organism is an obstacle to understanding the mechanisms behind its development and its ability to resist insecticides. analysis of the b. dorsalis transcriptome and its expression profile data is essential to extending the genetic information resources on this species, providing a shortcut that ... | 2011 | 22195006 |
transcriptional regulation of a chitinase gene by 20-hydroxyecdysone and starvation in the oriental fruit fly, bactrocera dorsalis. | insect chitinases are hydrolytic enzymes that are required for the degradation of glycosidic bonds of chitin. in this study, we identified and characterized a full-length cdna of the chitinase gene (bdcht2) in the oriental fruit fly, bactrocera dorsalis. the cdna contains an open reading frame (orf) of 1449 bp that encodes 483 amino acid residues and 126- and 296-bp non-coding regions at the 5'- and 3'-ends, respectively. the bdcht2 genome has four exons and three introns. the predicted molecula ... | 2013 | 24113584 |
molecular cloning, characterization and mrna expression of a chitin synthase 2 gene from the oriental fruit fly, bactrocera dorsalis (diptera: tephritidae). | chitin synthase (chs), a potential target for eco-friendly insecticides, plays an essential role in chitin formation in insects. in this study, a full-length cdna encoding chitin synthase 2 (bdchs2) was cloned and characterized in the oriental fruit fly, bactrocera dorsalis. the bdchs2 cdna had 4417 nucleotides, containing an open reading frame of 4122 nucleotides, which encoded 1373 amino acid residues with a predicted molecular weight of 158.5 kda. phylogenetic analysis with other insect chss ... | 2013 | 23965972 |
proteome analysis of male accessory gland secretions in oriental fruit flies reveals juvenile hormone-binding protein, suggesting impact on female reproduction. | in insects, the accessory gland proteins (acps) secreted by male accessory glands (mags) account for the majority of seminal fluids proteins. mixed with sperm, they are transferred to the female at mating and so impact reproduction. in this project, we identified 2,927 proteins in the mag secretions of the oriental fruit fly bactrocera dorsalis, an important agricultural pest worldwide, using lc-ms analysis, and all sequences containing open reading frames were analyzed using signalp. in total, ... | 2015 | 26582577 |
bdorcsp2 is important for antifeed and oviposition-deterring activities induced by rhodojaponin-iii against bactrocera dorsalis. | rhodojaponin-iii is a nonvolatile botanical grayanoid diterpene compound, which has antifeedant and oviposition deterrence effects against many kinds of insects. however, the molecular mechanism of the chemoreception process remains unknown. in this study, the important role of bdorcsp2 in the recognition of rhodojaponin-iii was identified. the full length cdna encoding bdorcsp2 was cloned from legs of bactrocera dorsalis. the results of expression pattern revealed that bdorcsp2 was abundantly e ... | 2013 | 24155937 |
bdorobp83a-2 mediates responses of the oriental fruit fly to semiochemicals. | the oriental fruit fly, bactrocera dorsalis (diptera: tephritidae), is one of the most destructive pests throughout tropical and subtropical regions in asia. this insect displays remarkable changes during different developmental phases in olfactory behavior between sexually immature and mated adults. the olfactory behavioral changes provide clues to examine physiological and molecular bases of olfactory perception in this insect. we comparatively analyzed behavioral and neuronal responses of b. ... | 2016 | 27761116 |
characterization of a β-adrenergic-like octopamine receptor in the oriental fruit fly, bactrocera dorsalis (hendel). | the biogenic amine octopamine plays a critical role in the regulation of many physiological processes in insects. octopamine transmits its action through a set of specific g-protein coupled receptors (gpcrs), namely octopamine receptors. here, we report on a β-adrenergic-like octopamine receptor gene (bdoctβr1) from the oriental fruit fly, bactrocera dorsalis (hendel), a destructive agricultural pest that occurs in north america and the asia-pacific region. as indicated by rt-qpcr, bdoctβr1 was ... | 2016 | 27669213 |
alternative splicing contributes to the coordinated regulation of ferritin subunit levels in bactrocera dorsalis (hendel). | a constant ratio of ferritin heavy chain homolog (hch) and light chain homolog (lch) subunits seems to be required to compose the ferritin heteropolymer protein in insects. however, the mechanism by which insect lch genes regulate protein levels remains unclear. we report that alternative promoters and alternative splicing contribute to maintaining a constant ratio of the two subunits, bdfer1hch and bdfer2lch (ferritin 1 hch and ferritin 2 lch), in bactrocera dorsalis, a notorious quarantine pes ... | 2014 | 24763285 |
transcriptomics and identification of the chemoreceptor superfamily of the pupal parasitoid of the oriental fruit fly, spalangia endius walker (hymenoptera: pteromalidae). | the oriental fruit fly, bactrocera dorsalis hendel, causes serious losses to fruit production and is one of the most economically important pests in many countries, including china, spalangia endius walker is a pupal parasitoid of various dipteran hosts, and may be considered a potentially important ectoparasitic pupal parasitoid of b. dorsalis. however, lack of genetic information on this organism is an obstacle to understanding the mechanisms behind its interaction with this host. analysis of ... | 2014 | 24505315 |
low diversity bacterial community and the trapping activity of metabolites from cultivable bacteria species in the female reproductive system of the oriental fruit fly, bactrocera dorsalis hendel (diptera: tephritidae). | our goal was to identify the bacteria inhabiting the reproductive system of the female oriental fruit fly, bactrocera dorsalis (hendel), and evaluate the chemotaxis of b. dorsalis to the metabolites produced by the bacteria. based on 16s rrna-based polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge), 18 operational taxonomic units (otus) were assigned to the five bacterial classes betaproteobacteria, alphaproteobacteria, gammaproteobacteria, bacilli and actinobacteria. n ... | 2012 | 22754363 |
identification of cultivable bacteria in the intestinal tract of bactrocera dorsalis from three different populations and determination of their attractive potential. | this study aimed to identify the cultivable bacteria inhabiting the intestinal tract of adult oriental fruit flies (bactrocera dorsalis) from laboratory-reared, laboratory sterile sugar-reared, and field-collected populations, and to evaluate the attractiveness of the metabolites produced by the above bacteria to their hosts. | 2014 | 23696217 |
in silico cloning and annotation of genes involved in the digestion, detoxification and rna interference mechanism in the midgut of bactrocera dorsalis [hendel (diptera: tephritidae)]. | as the second largest organ in insects, the insect midgut is the major tissue involved in the digestion of food and detoxification of xenobiotics, such as insecticides, and the first barrier and target for oral rna interference (rnai). in this study, we performed a midgut-specific transcriptome analysis in the oriental fruit fly, bactrocera dorsalis, an economically important worldwide pest, with many populations showing high levels of insecticide resistance. using high-throughput sequencing, 52 ... | 2013 | 23577657 |
captures of pest fruit flies (diptera: tephritidae) and nontarget insects in biolure and torula yeast traps in hawaii. | multilure traps were deployed in a hawaiian orchard to compare the attraction of economically important fruit flies and nontarget insects to the three-component biolure and torula yeast food lures. either water or a 20% propylene glycol solution was used to dissolve the torula yeast or as capture fluid in biolure traps. torula yeast in water was more attractive than biolure for male and female bactrocera cucurbitae (coquillett) and bactrocera dorsalis (hendel) and as attractive for ceratitis cap ... | 2010 | 22546461 |
antimicrobial peptide gene cecropin-2 and defensin respond to peptidoglycan infection in the female adult of oriental fruit fly, bactrocera dorsalis (hendel). | cecropins and defensins are important antimicrobial peptides in insects and are inducible after injection of immune triggers. in this study, we cloned the cdnas of two antimicrobial peptides (amps), cecropin-2 (bdcec-2) and defensin (bddef) from bactrocera dorsalis (hendel), a serious pest causing great economic losses to fruits and vegetables. the bdcec-2 sequence of 192bp encodes a protein of 63 amino acids residues with a predicted molecular weight of 6.78kd. the 282bp cdna of bddef encodes a ... | 2017 | 28089733 |
functional analysis of a nf-κb transcription factor in the immune defense of oriental fruit fly, bactrocera dorsalis hendel (diptera: tephritidae). | although some novel antimicrobial peptides (amp) have been successfully isolated from bactrocera dorsalis hendel, the mechanisms underlying the induction of these peptides are still elusive. the homolog of nf-κb transcription factor relish, designated as bdrelish, was cloned from b. dorsalis. the full length cdna of bdrelish is 3954 bp with an open reading frame that encodes 1013 amino acids. similar to drosophila relish and the mammalian p100, it is a compound protein containing a conserved rel ... | 2017 | 27871341 |
the pla2 gene mediates the humoral immune responses in bactrocera dorsalis (hendel). | the phospholipase a2 (pla2) gene encodes the enzyme that catalyzes the hydrolysis of phospholipids (pls) from the sn-2 position. however, little is known about its role in humoral immune responses. in this study, we investigated the expression profile of pla2 in different tissues and developmental stages in bactrocera dorsalis (hendel), and the results showed that the transcriptional level of pla2 was high in the egg and mature stage and in the testis tissue. bacterial infection increased the ex ... | 2017 | 27646139 |
the noa gene is functionally linked to the activation of the toll/imd signaling pathways in bactrocera dorsalis (hendel). | the noa gene is an essential gene encoding a very long chain fatty acid elongase. in this study, we cloned the noa gene of bactrocera dorsalis, which encodes a protein sharing 84.50% identity to the noa in drosophila melanogaster. the expression profiles indicated that the transcriptional level of noa was high at the egg stage and in the testis tissue. the results showed that noa expression was up-regulated after listeria monocytogenes, staphylococcus aureus and escherichia coli infection. silen ... | 2016 | 26404497 |
establishment of an rta-bddsx hybrid system for female-specific splicing that can affect the sex ratio of bactrocera dorsalis (hendel) after embryonic injection. | the oriental fruit fly, bactrocera dorsalis (hendel), a very destructive insect pest in many areas of asia, including taiwan, can cause significant damage by ovipositing in and larval feeding on many kinds of fruit. a female lethal system, combining the splicing property of doublesex (dsx) with the toxicity of ricin a chain (rta), has been developed. in this system, a modified rta is separated by bddsx intron 3; the expressed rna can only be spliced in females, with toxic effects, whereas the im ... | 2016 | 25656748 |
commensal bacteria aid mate-selection in the fruit fly, bactrocera dorsalis. | commensal bacteria influence many aspects of an organism's behaviour. however, studies on the influence of commensal bacteria in insect mate-selection are scarce. here, we present empirical evidence that commensal bacteria mediate mate-selection in the oriental fruit fly, bactrocera dorsalis. male flies were attracted to female flies, but this attraction was abolished when female flies were fed with antibiotics, suggesting the role of the fly's microbiota in mediating mate-selection. we show tha ... | 2016 | 27423980 |
bacterial communities associated with invasive populations of bactrocera dorsalis (diptera: tephritidae) in china. | the oriental fruit fly bactrocera dorsalis (hendel) is a destructive insect pest of a wide range of fruits and vegetables. this pest is an invasive species and is currently distributed in some provinces of china. to recover the symbiotic bacteria of b. dorsalis from different invasion regions in china, we researched the bacterial diversity of this fruit fly among one laboratory colony (guangdong, china) and 15 wild populations (14 sites in china and one site in thailand) using dna-based approach ... | 2016 | 27600786 |
association between changes in reproductive activity and d-glucose metabolism in the tephritid fruit fly, bactrocera dorsalis (hendel). | reproduction is an important life process in insects; however, few studies have attempted to demonstrate the association between reproductive activity and energy metabolism. to address this problem, we focused on the reproductive changes in bactrocera dorsalis males. we analyzed b. dorsalis male gene expression profiles during mating (dm), 3 h after mating (a3hm) and 12 h after mating (a12hm). gene annotation and pathway analyses of differentially expressed genes show that galactose metabolism a ... | 2014 | 25502224 |
the effect of dietary restriction on longevity, fecundity, and antioxidant responses in the oriental fruit fly, bactrocera dorsalis (hendel) (diptera: tephritidae). | recent studies in fruit flies have imposed dietary restriction (dr) by diluting yeast and have reported increased lifespan as the yeast-to-sugar ratio decreased. in this study, the effects of dr on the lifespan of bactrocera dorsalis were investigated using constant-feeding diets with different yeast:sugar ratios and an intermittent-feeding diet in which flies ate every sixth day. antioxidant enzyme activities and the malondialdehyde concentration were also measured in virgin females under const ... | 2013 | 23911350 |
the effects of rna interference targeting bactrocera dorsalis ds-bdrpl19 on the gene expression of rpl19 in non-target insects. | double-stranded rna (dsrna) designed to target pest genes emerges as a promising strategy for improving pest control. therefore, it is necessary to assess the effects of dsrna on non-target insects, such as native enemies and beneficial insects, to determine the environmental safety of such treatments. in this paper, we investigated the effects of dsrna targeting rpl19 from bactrocera dorsalis on non-target insects in citrus ecological systems by feeding the dsrna to bactrocera minax, apis melli ... | 2015 | 25567188 |
effects of neem leaf dust and a commercial formulation of a neem compound on the longevity, fecundity and ovarian development of the melon fly, bactrocera cucurbitae (coquillett) and the oriental fruit fly, bactrocera dorsalis (hendel) (diptera: tephritidae). | neem leaf dust and a commercial formulation of neem were tested on adult bactrocera cucurbitae (coquillett) and bactrocera dorsalis (hendel) to determine their effects on the longevity, fecundity and ovarian development. different combinations of neem leaf dust and a commercial formulation of a neem compound incorporated with sugar solution and adult rearing diets were tested. the laboratory tests showed that ingestion of neem can significantly reduced the longevity and fertility of both the fly ... | 2007 | 19093477 |
the complete nucleotide sequence of the mitochondrial genome of bactrocera minax (diptera: tephritidae). | the complete 16,043 bp mitochondrial genome (mitogenome) of bactrocera minax (diptera: tephritidae) has been sequenced. the genome encodes 37 genes usually found in insect mitogenomes. the mitogenome information for b. minax was compared to the homologous sequences of bactrocera oleae, bactrocera tryoni, bactrocera philippinensis, bactrocera carambolae, bactrocera papayae, bactrocera dorsalis, bactrocera correcta, bactrocera cucurbitae and ceratitis capitata. the analysis indicated the structure ... | 2014 | 24964138 |
alterations of the acetylcholinesterase enzyme in the oriental fruit fly bactrocera dorsalis are correlated with resistance to the organophosphate insecticide fenitrothion. | alterations of the structure and activity of the enzyme acetylcholinesterase (ache) leading to resistance to organophosphate insecticides have been examined in the oriental fruit fly, bactrocera dorsalis (hendel), an economic pest of great economic importance in the asia-pacific region. we used affinity chromatography to purify ache isoenzymes from heads of insects from lines showing the phenotypes of resistance and sensitivity to insecticide treatments. the ache enzyme from a strain selected fo ... | 2008 | 18207076 |
the complete nucleotide sequence of the mitochondrial genome of the oriental fruit fly, bactrocera dorsalis (diptera: tephritidae). | the complete mitochondrial genome of the oriental fruit fly bactrocera dorsalis s.s. has been sequenced, and is here described and compared with the homologous sequences of bactrocera oleae and ceratitis capitata. the genome is a circular molecule of 15,915 bp, and encodes the set of 37 genes generally found in animal mitochondrial genomes. the structure and organization of the molecule is typical and similar to the two closely related species b. oleae and c. capitata, although it presents an in ... | 2007 | 17433576 |
relative tolerance of six bactrocera (diptera: tephritidae) species to phytosanitary cold treatment. | to compare relative cold treatment tolerance across the economically important tephritid fruit flies (diptera: tephritidae), bactrocera carambolae drew & hancock, bactrocera correcta (bezzi), bactrocera cucurbitae (coquillett), four populations of bactrocera dorsalis (hendel), bactrocera zonata (saunders), and bactrocera tryoni (froggatt), eggs (in vitro), and larvae (in infested fruit or on carrot diet) were cold treated at 2.0 ± 0.2 °c for selected durations. the study was performed to assess ... | 2016 | 27660425 |
the mitochondrial genome of the peach fruit fly, bactrocera zonata (saunders) (diptera: tephritidae): complete dna sequence, genome organization, and phylogenetic analysis with other tephritids using next generation dna sequencing. | mitochondrial genome can provide information for genomic structure as well as for phylogenetic analysis and evolutionary biology. the complete 15,935 bp mitochondrial genome of bactrocera zonata (diptera: tephritidae), is assembled from illumina miseq read data. the mitogenome information for b. zonata was compared to the homologous sequences of other tephritids. annotation indicated that the structure and orientation of 13 protein coding genes (pcgs), 22 trna and 2 rrna sequences were typical o ... | 2015 | 26031235 |
teratogenic effect of lead acetate on bactrocera dorsalis and bactrocera zonata. | bactrocera dorsalis and bactrocera zonata were studied under the effect of lead acetate for 48 hours exposure at larval stages. since, lead is widely distributed industrial pollutant in the world. it is significant environmental pollutant which contaminates foods, water, soil and air and insect could get easily the influence of such pollutants. therefore, the teratogenic effects of lead (lead acetate) on external morphology on fruit flies was determined. the effects of lead acetate, in the conce ... | 2012 | 22459456 |
molecular characterizations of natalisin and its roles in modulating mating in the oriental fruit fly, bactrocera dorsalis (hendel). | initially, natalisin (ntl) was identified from three holometabolous insect species, drosophila melanogaster, tribolium castaneum and bombyx mori, and was documented to regulate reproductive behaviours in d. melanogaster and t. castaneum. in this study, we report the sequences of the ntl precursor and its receptor (ntlr) from an important agricultural pest, bactrocera dorsalis (hendel). ntlr is a typical g-protein coupled receptor and phylogenetic analysis showed that b. dorsalis ntlr was closely ... | 2017 | 27862548 |
weathering and chemical degradation of methyl eugenol and raspberry ketone solid dispensers for detection, monitoring, and male annihilation of bactrocera dorsalis and bactrocera cucurbitae (diptera: tephritidae) in hawaii. | solid male lure dispensers containing methyl eugenol (me) and raspberry ketone (rk), or mixtures of the lures (me + rk), and dimethyl dichloro-vinyl phosphate (ddvp) were evaluated in area-wide pest management bucket or jackson traps in commercial papaya (carica papaya l.) orchards where both oriental fruit fly, bactrocera dorsalis (hendel), and melon fly, bactrocera cucurbitae (coquillett), are pests. captures of b. dorsalis with fresh wafers in jackson and bucket traps were significantly highe ... | 2015 | 26470301 |
assessment of attractiveness of cassava as a roosting plant for the melon fly, bactrocera cucurbitae, and the oriental fruit fly, b. dorsalis. | application of bait spray to crop borders is a standard approach for suppression of melon fly, bactrocera cucurbitae (coquillett) (diptera: tephritidae) populations and may also be of value for suppression of oriental fruit fly, b. dorsalis (hendel) populations. establishment of preferred roosting hosts as crop borders may help to improve suppression of both fruit fly species by providing sites for bait spray applications. in an area-wide b. cucurbitae suppression trial, the question was raised ... | 2011 | 21526935 |
response of ceratitis capitata, bactrocera dorsalis, and bactrocera cucurbitae (diptera: tephritidae) to metabolic stress disinfection and disinfestation treatment. | metabolic stress disinfection and disinfestation (msdd) is a postharvest treatment designed to control pathogens and arthropod pests on commodities that combines short cycles of low pressure/vacuum and high co2 with ethanol vapor. experiments were conducted to evaluate the effect of msdd treatment on various life stages of ceratitis capitata (wiedemann), mediterranean fruit fly; bactrocera dorsalis hendel, oriental fruit fly; and bactrocera cucurbitae coquillett, melon fly, in petri dishes and i ... | 2011 | 21404842 |
managing oriental fruit fly (diptera: tephritidae), with spinosad-based protein bait sprays and sanitation in papaya orchards in hawaii. | the efficacy of gf-120 nf naturalyte fruit fly bait in combination with field sanitation was assessed as a control for female oriental fruit fly, bactrocera dorsalis (hendel) (diptera: tephritidae), in papaya (carica papaya l.) orchards in hawaii. three different bait spray regimes were evaluated: every row (high use of the bait), every fifth row (moderate use), and every 10th row (low use). orchard plots in which no bait was applied served as controls. for five of the seven biweekly periods tha ... | 2009 | 19610428 |
evaluation of splat with spinosad and methyl eugenol or cue-lure for "attract-and-kill" of oriental and melon fruit flies (diptera: tephritidae) in hawaii. | specialized pheromone and lure application technology (splat) methyl eugenol (me) and cue-lure (c-l) "attract-and-kill" sprayable formulations containing spinosad were compared with other formulations under hawaiian weather conditions against oriental fruit fly, bactrocera dorsalis (hendel), and melon fly, bactrocera cucurbitae (coquillett) (diptera: tephritidae), respectively. field tests were conducted with three different dispensers (min-u-gel, acti-gel, and splat) and two different insectici ... | 2008 | 18613576 |
assessment of attractiveness of plants as roosting sites for the melon fly, bactrocera cucurbitae, and oriental fruit fly, bactrocera dorsalis. | the use of toxic protein bait sprays to suppress melon fly, bactrocera cucurbitae (coquillett) (diptera: tephritidae), populations typically involves application to vegetation bordering agricultural host areas where the adults seek shelter ("roost"). although bait spray applications for suppression of oriental fruit fly, bactrocera dorsalis (hendel), populations have traditionally been applied to the host crop, rather than to crop borders, roosting by oriental fruit flies in borders of some crop ... | 2007 | 20334596 |
life history data on the fly parasitoids aleochara nigra kraatz and a. asiatica kraatz (coleoptera: staphylinidae), and their potential application in forensic entomology. | knowledge of the developmental time of the immature stages of necrophagous flies has been the main tool for estimating minimum post-mortem intervals (min pmis) in forensic entomology. many parasitic insects can alter the development of immature stages of flies and thus affect min pmi estimates. the larvae of most species of aleochara rove beetles are ectoparasitoids of the pupae of cyclorrhapha flies. among them, some species that parasitise necrophagous flies may have forensic importance. two t ... | 2013 | 24053864 |
trap capture of three economically important fruit fly species (diptera: tephritidae): evaluation of a solid formulation containing multiple male lures in a hawaiian coffee field. | invasive fruit flies (diptera: tephritidae) pose a global threat to agriculture through direct damage to food crops and the accompanying trade restrictions that often result. early detection is vital to controlling fruit flies, because it increases the probability of limiting the growth and spread of the invasive population and thus may greatly reduce the monetary costs required for eradication or suppression. male-specific lures are an important component of fruit fly detection, and three such ... | 2012 | 22928297 |
attraction of ethyl ether extracts of 232 botanicals to oriental fruit flies, melon flies, and mediterranean fruit flies. | the mediterranean fruit fly, ceratitis capitata (wiedemann), the melon fly, dacus cucurbitae coquillett, and the oriental fruit fly, d. dorsalis hendel, three hawaiian tephritids of economic importance, were exposed to traps each containing one of 232 ethyl ether extracts of air-dried botanicals. the principal concern was to establish which extracts were attractive judged on the basis of an index determined by the number of times more flies were attracted to the trap baited with water and extrac ... | 2008 | 1134212 |
the short neuropeptide f modulates olfactory sensitivity of bactrocera dorsalis upon starvation. | the insect short neuropeptide f (snpf) family has been shown to modulate diverse physiological processes, such as feeding, appetitive olfactory behavior, locomotion, sleep homeostasis and hormone release. in this study, we identified the snpf (bdsnpf) and its receptor (bdsnpfr) in an important agricultural pest, the oriental fruit fly bactrocera dorsalis (hendel). afterwards, the receptor cdna was functionally expressed in chinese hamster ovary cell lines. activation of bdsnpfr by snpf peptides ... | 2017 | 28377097 |
a role of corazonin receptor in larval-pupal transition and pupariation in the oriental fruit fly bactrocera dorsalis (hendel) (diptera: tephritidae). | corazonin (crz) is a neuropeptide hormone, but also a neuropeptide modulator that is internally released within the cns, and it has a widespread distribution in insects with diverse physiological functions. here, we identified and cloned the cdnas of bactrocera dorsalis that encode crz and its receptor crzr. mature bdcrz has 11 residues with a unique ser(11) substitution (instead of the typical asn) and a his in the evolutionary variable position 7. the bdcrzr cdna encodes a putative protein of ... | 2017 | 28261106 |
role of a tachykinin-related peptide and its receptor in modulating the olfactory sensitivity in the oriental fruit fly, bactrocera dorsalis (hendel). | insect tachykinin-related peptide (trp), an ortholog of tachykinin in vertebrates, has been linked with regulation of diverse physiological processes, such as olfactory perception, locomotion, aggression, lipid metabolism and myotropic activity. in this study, we investigated the function of trp (bdtrp) and its receptor (bdtrpr) in an important agricultural pest, the oriental fruit fly bactrocera dorsalis. bdtrpr is a typical g-protein coupled-receptor (gpcr), and it could be activated by the pu ... | 2017 | 27923683 |
hot-water immersion quarantine treatment against mediterranean fruit fly and oriental fruit fly (diptera: tephritidae) eggs and larvae in litchi and longan fruit exported from hawaii. | immersion of litchi fruit in 49 degrees c water for 20 min followed by hydrocooling in ambient (24 +/- 4 degrees c) temperature water for 20 min was tested as a quarantine treatment against potential infestations of mediterranean fruit fly, ceratitis capitata (wiedemann); and oriental fruit fly, bactrocera dorsalis hendel, eggs or larvae in hawaiian litchi, litchi chinensis sonnerat. the 49 degrees c hot-water immersion of litchi provided probit 9 (99.9968% mortality with >95% confidence) quaran ... | 2007 | 17849856 |
interspecific competition between ceratitis capitata and two bactrocera spp. (diptera: tephritidae) evaluated via adult behavioral interference under laboratory conditions. | the mediterranean fruit fly, ceratitis capitata (wiedemann), is considered one of the most invasive tephritid species. it has spread and established populations successfully throughout many of the tropical temperate regions, partially owing to the increase in global trading activity that facilitates diffusion of species. however, c. capitata has never been detected in china, even though some areas of the country have favorable climate and ample food resources. historically, some researchers have ... | 2017 | 28334323 |
functional analysis of five trypsin-like protease genes in the oriental fruit fly, bactrocera dorsalis (diptera: tephritidae). | insect midgut proteases catalyze the release of free amino acids from dietary proteins and are essential for insect normal development. to date, digestive proteases as potential candidates have made great progress in pest control. to clarify the function of trypsin-like protease genes in the digestive system of bactrocera dorsalis, a serious pest of a wide range of tropical and subtropical fruit and vegetable crops, five trypsin genes (bdtry1, bdtry2, bdtry3, bdtry4 and bdtry5) were identified f ... | 2017 | 28187831 |
gut symbiont enhances insecticide resistance in a significant pest, the oriental fruit fly bactrocera dorsalis (hendel). | symbiotic bacteria affect insect physiology and ecology. they may also mediate insecticide resistance within their hosts and thereby impact pest and vector control practices. here, we document a novel mechanism of insecticide resistance in which a gut symbiont of the tephritid pest fruit fly bactrocera dorsalis enhances resistance to the organophosphate insecticide trichlorphon. | 2017 | 28143582 |
phytosanitary treatments against bactrocera dorsalis (diptera: tephritidae): current situation and future prospects. | bactrocera dorsalis (hendel) (diptera: tephritidae) is arguably the most important tephritid attacking fruits after ceratitis capitata (wiedemann) (diptera: tephritidae). in 2003 it was found in africa and quickly spread to most of the sub-saharan part of the continent, destroying fruits and creating regulatory barriers to their export. the insect is causing new nutritional and economic losses across africa, as well as the losses it has caused for decades in infested areas of asia, new guinea, a ... | 2017 | 28028169 |
factors influencing aversive learning in the oriental fruit fly, bactrocera dorsalis. | parameters such as the intensity of conditioned and unconditioned stimuli, the inter-trial interval, and starvation time can influence learning. in this study, the parameters that govern aversive learning in the oriental fruit fly, bactrocera dorsalis, a serious pest of fruits and vegetables, were examined. male flies were trained to associate the attractive odorant methyl eugenol, a male lure, with a food punishment, sodium chloride solution, and the conditioned suppression of the proboscis-ext ... | 2017 | 27909789 |
tephritid integrative taxonomy: where we are now, with a focus on the resolution of three tropical fruit fly species complexes. | accurate species delimitation underpins good taxonomy. formalization of integrative taxonomy in the past decade has provided a framework for using multidisciplinary data to make species delimitation hypotheses more rigorous. we address the current state of integrative taxonomy by using as a case study an international project targeted at resolving three important tephritid species complexes: bactrocera dorsalis complex, anastrepha fraterculus complex, and ceratitis far (c. fasciventris, c. anona ... | 2017 | 27813666 |
high genetic diversity in the offshore island populations of the tephritid fruit fly bactrocera dorsalis. | geographic isolation is an important factor that limit species dispersal and thereby affects genetic diversity. because islands are often small and surrounded by a natural water barrier to dispersal, they generally form discrete isolated habitats. therefore, islands may play a key role in the distribution of the genetic diversity of insects, including flies. | 2016 | 27737670 |
genetic analysis of oriental fruit fly, bactrocera dorsalis (diptera: tephritidae) populations based on mitochondrial cox1 and nad1 gene sequences from india and other asian countries. | the study examined the genetic diversity and demographic history of bactrocera dorsalis, a destructive and polyphagous insect pest of fruit crops in diverse geographic regions of india. 19 widely dispersed populations of the fly from india and other asian countries were analysed using partial sequences of mitochondrial cytochrome oxidase i (cox1) and nadh dehydrogenase 1 (nad1) genes to investigate genetic diversity, genetic structure, and demographic history in the region. genetic diversity ind ... | 2016 | 27699519 |
demographic analysis of sex ratio on population growth of bactrocera dorsalis (diptera: tephritidae) with discussion of control efficacy using male annihilation. | the life table data for the oriental fruit fly, bactrocera dorsalis (hendel), at different adult sex ratios (1♀: 1♂, 1♀: 50♂, 50♀: 1♂ free-choice mating, and 50♀: 1♂ no-choice mating) were collected to determine the effects of sex-ratio manipulation on current pest control procedures. at 1♀: 1♂, females mated, on average, 2.3 times during their lifetime with a mean fecundity (f) of 1,122 eggs. the net reproductive rate (r0), intrinsic rate of increase (r), finite rate (λ), and mean generation ti ... | 2016 | 27694182 |
biology of fopius arisanus (hymenoptera: braconidae) in two species of fruit flies. | fopius arisanus (sonan, 1932) (hymenoptera: braconidae) is an egg-larval parasitoid used in control programs of bactrocera dorsalis (hendel) and ceratitis capitata (wiedemann). in brazil, c. capitata and anastrepha fraterculus (wiedemann) are considered the main tephritid pests of exotic and indigenous fruits. the objective of this study was to study the biology of f. arisanus in c. capitata and a. fraterculus eggs of the two fruit fly species were used to determine the parasitism rate, number o ... | 2016 | 27638954 |
identification and characterization of sex-biased micrornas in bactrocera dorsalis (hendel). | micrornas (mirnas) are a class of endogenous small non-coding rnas that regulate various biological processes including sexual dimorphism. the oriental fruit fly bactrocera dorsalis is one of the most destructive agricultural insect pests in many asian countries. however, no mirnas have been identified from the separate sex and gonads to elucidate sex gonad differentiation in b. dorsalis. in this study, we constructed four small rna libraries from whole body of females, males (except ovaries and ... | 2016 | 27441641 |
identification of a carboxylesterase associated with resistance to naled in bactrocera dorsalis (hendel). | compared to other organophosphate-resistant and -susceptible (s) lines of bactrocera dorsalis, the carboxylesterase (cbe) bde5 in the naled-resistant (nal-r) line has been found to possess remarkable quantitative elevation. our study attempts to identify the role of bde5 in naled resistance, and we discovered several points of interest. firstly, activity staining on native page revealed that the percentage of flies with intensive bde5 bands in the nal-r line was substantially higher than in the ... | 2016 | 27265823 |
functional characterization of an α-esterase gene involving malathion detoxification in bactrocera dorsalis (hendel). | extensive use of insecticides in many orchards has prompted resistance development in the oriental fruit fly, bactrocera dorsalis (hendel). in this study, a laboratory selected strain of b. dorsalis (mr) with a 21-fold higher resistance to malathion was used to examine the resistance mechanisms to this organophosphate insecticide. carboxylesterase (care) was found to be involved in malathion resistance in b. dorsalis from the synergism bioassay by care-specific inhibitor triphenylphosphate (tpp) ... | 2016 | 27155483 |
genetically engineered ricin suppresses bactrocera dorsalis (diptera: tephritidae) based on demographic analysis of group-reared life table. | the oriental fruit fly, bactrocera dorsalis (hendel), reduces the quantity and quality of many host fruits through the process of oviposition and larval feeding, and this insect has been considered a major insect pest in several asian countries for decades. using an earlier-developed, female-specific system that combines the toxicity of the ricin a chain (rta) and the alternative rna splicing property of doublesex (bddsx), we show that transgenic male flies harboring the rta-bddsx transgene unev ... | 2016 | 27122495 |
larval x-ray irradiation influences protein expression in pupae of the oriental fruit fly, bactrocera dorsalis. | the sterile insect technique (sit) was developed to eradicate the new world screwworm from the southern united states and mexico, and became a component of many area-wide integrated pest management programs, particularly useful in managing tephritid fruit flies. sit is based on the idea of rearing and sterilizing male pests, originally by ionizing radiation, and then releasing into field, where they compete for and mate with wild females. mating with sterile males leads to reduced fecundity to l ... | 2016 | 27079560 |