| identification of colletotrichum species responsible for anthracnose of strawberry based on the internal transcribed spacers of the ribosomal region. | in recent years, different molecular techniques have led to an important progress in the characterisation of colletotrichum species, but there are no available methods which permit the easy identification of colletotrichum strains and their assignation to classical species. in the present work, the restriction patterns generated from the region spanning the internal transcribed spacers (its1 and its2) and the 5.8s rrna gene, were used to identify a total of 80 strains of colletotrichum, the majo ... | 2000 | 10913873 |
| triterpene phytoalexins from strawberry fruit. | strawberry cv. houkouwase is resistant to infection by colletotrichum fragariae. the formation of antifungal compounds was observed in unripe fruit which had been wounded and inoculated with conidia of c. musae. three antifungal compounds were isolated and identified as euscaphic acid, tormentic acid and myrianthic acid. myrianthic acid inhibited the growth of c. musae at 3 microg, and euscaphic and tormentic acids showed inhibitory effects at 100 microg. a quantitative analysis of their content ... | 2000 | 10993160 |
| phytotoxic and fungitoxic activities of the essential oil of kenaf (hibiscus cannabinus l.) leaves and its composition. | the chemical composition of the essential oil of kenaf (hibiscus cannabinus) was examined by gc-ms. fifty-eight components were characterized from h. cannabinus with (e)-phytol (28.16%), (z)-phytol (8.02%), n-nonanal (5.70%), benzene acetaldehyde (4.39%), (e)-2-hexenal (3.10%), and 5-methylfurfural (3.00%) as the major constituents. the oil was phytotoxic to lettuce and bentgrass and had antifungal activity against colletotrichum fragariae, colletotrichum gloeosporioides, and colletotrichum accu ... | 2001 | 11513663 |
| thiourea derivatives and their nickel(ii) and platinum(ii) complexes: antifungal activity. | we have synthesized two thiourea derivatives of methyl anthranilate (1, 2) and their complexes with nickel (3) and platinum(ii) (4). we have also prepared the complexes of nickel(ii) with two benzoylthiourea derivatives (5, 6). the obtained compounds were characterized by elemental analysis, spectroscopic methods (ft-ir, uv-vis, nmr), mass spectrometry and thermal analysis. compound 1, c(20)h(23)n(3)o(2)s, crystallizes in monoclinic space group p21/n, with z=4, and unit cell parameters, a=8.8042 ... | 2002 | 11931966 |
| ph modulation of zopfiellin antifungal activity to colletotrichum and botrytis. | zopfiellin, a novel cyclooctanoid natural product isolated from zopfiella curvata no. 37-3, was evaluated in a 96-well microtiter assay for fungicidal activity against botrytis cinerea, colletotrichum acutatum, colletotrichum fragariae, colletotrichum gloeosporioides, and fusarium oxysporum. zopfiellin exhibited ph-dependent activity, with the most mycelial growth inhibition demonstrated at ph 5.0. mass spectrometry and nuclear magnetic resonance spectroscopy studies indicated that zopfiellin un ... | 2002 | 12428951 |
| activity of quinones on colletotrichum species. | the antifungal activity of 1,4-naphthoquinones, 1,2-naphthoquinones, 1,4-benzoquinones, and anthraquinones from our natural products collection was tested by direct bioautography to identify natural products with potential use in agricultural fungal pathogen control. quinones demonstrated good to moderate antifungal activity against colletotrichum spp. colletotrichum fragariae was the most sensitive species to quinone-based chemistry, colletotrichum gloeosporioides had intermediate sensitivity, ... | 2003 | 12797750 |
| isolation and identification of antifungal and antialgal alkaloids from haplophyllum sieversii. | bioassay-guided fractionation of the hexane/ethyl acetate/water (h/etoac/h2o) crude extract of the aerial parts of haplophyllum sieversii was performed because of preliminary screening data that indicated the presence of growth inhibitory components against colletotrichum fragariae, colletotrichum gloeosporioides, and colletotrichum acutatum. fractionation was directed using bioautographical methods resulting in the isolation of the bioactive alkaloids flindersine, anhydroevoxine, haplamine, and ... | 2005 | 16190626 |
| phytotoxic and antifungal compounds from two apiaceae species, lomatium californicum and ligusticum hultenii, rich sources of z-ligustilide and apiol, respectively. | the seeds of two apiaceae species, ligusticum hultenii and lomatium californicum, were investigated. preliminary bioassays indicated that methylene chloride extracts of seeds of both species contained selective phytotoxic activity against monocots and antifungal activity against colletotrichum fragariae. active constituents were isolated by bioassay-guided fractionation, and the structures were elucidated by nmr and gc-ms as apiol and z-ligustilide, isolated from l. hultenii and l. californicum, ... | 2005 | 16222793 |
| algicidal and antifungal compounds from the roots of ruta graveolens and synthesis of their analogs. | bioassay-guided fractionation of the ethyl acetate extract of ruta graveolens roots yielded rutacridone epoxide with potent selective algicidal activity towards the 2-methyl-isoborneol (mib)-producing blue-green alga oscillatoria perornata, with relatively little effect on the green alga selenastrum capricornutum. the diol-analog of rutacridone epoxide, gravacridondiol, which was also present in the same extract, had significantly less activity towards o. perornata. rutacridone epoxide also show ... | 2005 | 16271733 |
| antifungal activity of thiophenes from echinops ritro. | extracts from 30 plants of the greek flora were evaluated for their antifungal activity using direct bioautography assays with three colletotrichum species. among the bioactive extracts, the dichloromethane extract of the radix of echinops ritro (asteraceae) was the most potent. bioassay-guided fractionation of this extract led to the isolation of eight thiophenes. antifungal activities of isolated compounds together with a previously isolated thiophene from echinops transiliensis were first eva ... | 2006 | 16506815 |
| characterization of volatile constituents of haplopappus greenei and studies on the antifungal activity against phytopathogens. | essential oil of haplopappus greenei a. gray was obtained by hydrodistillation of aerial parts, which were subsequently analyzed by gas chromatography and gas chromatography-mass spectrometry. major components were identified as carvacrol (8.7%), beta-pinene (7.6%), trans-pinocarveol (6.2%), and caryophyllene oxide (5.8%), respectively. in total, 104 components representing 84.9% of the investigated essential oil were characterized. furthermore, the essential oil was evaluated for antimalarial, ... | 2006 | 16608244 |
| chemical composition and antifungal activity of salvia macrochlamys and salvia recognita essential oils. | essential oils of salvia macrochlamys and salvia recognita were obtained by hydrodistillation of dried aerial parts and characterized by gas chromatography and gas chromatography-mass spectrometry. one hundred and twenty identified constituents representing 97.7% in s. macrochlamys and 96.4% in s. recognita were characterized, and 1,8-cineole, borneol, and camphor were identified as major components of the essential oils. the oils were evaluated for their antimalarial, antimicrobial, and antifun ... | 2006 | 16939314 |
| cloning and expression analysis of two beta-1,3-glucanase genes from strawberry. | we isolated from strawberry (fragariae x ananassa duch) a genomic clone of a beta-1,3-glucanase gene, designated as fabg2-2. in addition, a related cdna clone, designated as fabg2-3, was also isolated. fabg2-2 and fabg2-3 are similar in their coding regions, except that fabg2-2 does not appear to contain a signal peptide coding sequence. the 5' and 3' flanking regions of fabg2-2 and fabg2-3 are differentt. using real-time pcr, the expression patterns of fabg2-3 and a previously isolated beta-1,3 ... | 2006 | 16949959 |
| isolation and identification of antifungal fatty acids from the basidiomycete gomphus floccosus. | bioautography of extracts of the fruiting bodies of the basidiomycete gomphus floccosus (schw.) singer indicated the presence of fungitoxic compounds in the ethyl acetate fraction against the plant pathogens colletotrichum fragariae, colletotrichum gloeosporioides, and colletotrichum acutatum. bioassay-guided fractionation of this fraction resulted in the isolation of the bioactive fatty acids (9 s,10 e,12 z)-9-hydroxy-10,12-octadecadienoic acid (1), (9 e,11 z)-13-oxo-9,11-octadecadienoic acid ( ... | 2008 | 18557621 |
| antagonism of bacillus spp. isolated from marine biofilms against terrestrial phytopathogenic fungi. | we aimed at determining the antagonistic behavior of bacteria derived from marine biofilms against terrestrial phytopathogenic fungi. some bacteria closely related to bacillus mojavensis (three isolates) and bacillus firmus (one isolate) displayed antagonistic activity against colletotrichum gloeosporioides atcc 42374, selected as first screen organism. the four isolates were further quantitatively tested against c. gloeosporioides, colletotrichum fragariae, and fusarium oxysporum on two culture ... | 2009 | 18931878 |
| antifungal metabolites from the roots of diospyros virginiana by overpressure layer chromatography. | a preparative overpressure layer chromatography (oplc) method was successfully used for the separation of two new natural compounds, 4-hydroxy-5,6-dimethoxynaphthalene-2-carbaldehyde (1) and 12,13-didehydro-20,29-dihydrobetulin (2) together with nine known compounds, including 7-methyljuglone (3), diospyrin (4), isodiospyrin (5), shinanolone (6), lupeol (7), betulin (8), betulinic acid (9), betulinaldehyde (10), and ursolic acid (11) from the acetone extract of the roots of diospyros virginiana. ... | 2011 | 22162171 |