| plastic sectioning for light microscopy of pyrenomycetes. | in preparation for light microscopy, ascocarps of sordaria fimicola ces. & denot. were embedded in spurr's medium and sectioned at 1-1.5 mum on an ultramicrotome. sections were floated on giemsa staining solution at 60 c for 10-30 min, washed in distilled water, affixed to slides by drying, and mounted in immersion oil. best preservation of the delicate sterile tissues of the centrum was obtained by fixation in 1% kmno4 for 2.5-3 hr, followed by the giemsa stain. this method is suggested for fut ... | 1975 | 52918 |
| prevention of 5-fluorouracil-caused growth inhibition in sordaria fimicola. | growth (dry weight accumulation) of sordaria fimicola in standing liquid culture (sucrose-nitrate-salts-vitamins) is inhibited by the presence of 5 mum 5-fluorouracil in the medium. this inhibition is completely prevented by uracil, deoxyuridine, and 5-bromouracil, partly prevented (40 to 90% of growth observed without 5-fluorouracil) by uridine, thymidine, and 5-bromodeoxyuridine, and slightly prevented by trifluorothymine, cytosine, cytidine, deoxycytidine, and 5-methylcytosine (all at 0.5 to ... | 1977 | 848926 |
| control of the pattern of perithecium development in sordaria fimicola on agar medium. | in a sordaria fimicola (rob.) ces. and de not. colony grown on agar medium in a petri plate, perithecia developed in a narrow band around the plate edge after the colony margin reached the edge. physical wounding of the colony carried out shortly before or during the time perithecia were developing around the plate edge stimulated perithecium development in the wound area. diffusion barriers were created by cutting small trenches in the agar parallel to the plate edge. the trenches were made at ... | 1975 | 1158169 |
| composition of eukaryotic gene loci regarding gene conversion units and the presence or the absence of intralocus reciprocal recombination. | in sordaria fimicola, the g-locus is composed of a single convertron and does not show intralocus reciprocal recombination. on the contrary, the i-locus is composed of multiple convertrons and shows genuine intralocus reciprocal recombination. | 1989 | 2698686 |
| a general method for identifying correct solutions in the quantitative analysis of gene conversion data. | past attempts to obtain values for meiotic parameters relating to hybrid dna formation and the correction of mismatched bases in hybrid dna have not given unique solutions unless various simplifying assumptions were made. a method is given for identifying correct sets of solutions after calculating the frequency of hybrid dna formation at a heterozygous site and using the fact that closely linked sites within a locus have very similar hybrid dna formation frequencies. the method is illustrated w ... | 1986 | 3801967 |
| gene conversion at the gray locus of sordaria fimicola: fit of the experimental data to a hybrid dna model of recombination. | a hybrid dna (hdna) model of recombination has been algebraically formulated, which allows the prediction of frequencies of postmeiotic segregation and conversion of a given allele and their probability of being associated with a crossing over. the model considered is essentially the "aviemore model." in contrast to some other interpretations of recombination, it states that gene conversion can only result from the repair of heteroduplex hdna, with postmeiotic segregation resulting from unrepair ... | 1985 | 3979816 |
| characterization of rna populations in ascobolus immersus, sordaria fimicola and podospora anserina. | | 1974 | 4457972 |
| the fine structure of ascospore wall formation in sordaria fimicola. | | 1972 | 5015589 |
| alteration of gene conversion patterns in sordaria fimicola by supplementation with dna bases. | supplementation with dna bases in crosses of sordaria fimicola heterozygous for spore color markers (g(1), h(2)) within the gray-spore (g) locus has been found to cause significant alterations in patterns of gene conversion at the two mutant sites. each base had its own characteristic effect in altering the conversion pattern, and responses of the two mutant sites to the four bases were different in several ways. also, the responses of the two involved chromatids of the meiotic bivalent were dif ... | 1970 | 5273454 |
| genetics of sordaria fimicola. vii. gene conversion at the g locus in interallelic crosses. | | 1969 | 5371017 |
| related and unrelated changes in conversion and recombination frequencies with temperature in sordaria fimicola, and their relevance to hybrid-dna models of recombination. | | 1969 | 5371018 |
| effect of carbon-nitrogen ratios on production of perithecia by sordaria fimicola. | | 1971 | 5555521 |
| gene conversion: temperature data from sordaria fimicola on the correction of mis-paired bases. | | 1968 | 5639149 |
| growth and sporulation of a pyrimidine spore color mutant of sordaria fimicola. | a nonautonomous spore color mutant of sordaria fimicola is a pyrimidine auxotroph that produces hyaline nonviable ascospores. uracil, uridine, and cytidine are more effective growth factors than cytosine and thymine and, in high concentrations, render the mutant self-fertile by inducing the ascospores to resume development and maturation. crosses with the unlinked arginine non-autonomus spore color mutant st-59 yielded the double mutant st-59 pyr that requires both arginine and a pyrimidine for ... | 1967 | 6020047 |
| genetics of sordaria fimicola. vi. gene conversion at the g locus in mutant x wild type crosses. | | 1967 | 6082621 |
| coprophilous fungi of the horse. | a total of 1267 microfungi, including 35 myxomycetes, were recorded from the fecal samples of the 60 horses; of these 395 were found on 20 saddle-horse feces, 363 on 20 race-horses and 509 on 20 working horses. eighty two species representing 53 genera were recorded; of these 7 were zygomycetes, 18 ascomycetes, 1 basidiomycetes and 25 fungi imperfecti: 2 myxomycetes. common coprophilous fungi are in decreasing order pilobolus kleinii, saccobolus depauperatus, mucor hiemalis, lasiobolus ciliatus, ... | 1981 | 7242651 |
| phylogenetic ordinal placement based on rdna sequences of the freshwater genera ophioceras and pseudohalonectria. | the ordinal placement of two closely related freshwater genera, ophioceras and pseudohalonectria, was assessed by using phylogenetic analysis of morphological characters, partial sequences of the large subunit ribosomal dna and restriction site variations in the internal transcribed spacer (its). the two genera have some morphological features that are used to define taxa in both the sordariales and diaporthales, and, hence, their phylogenetic relationships are unclear. equally weighted analyses ... | 1995 | 7553269 |
| intergeneric complementation of a circadian rhythmicity defect: phylogenetic conservation of structure and function of the clock gene frequency. | the neurospora crassa frequency locus encodes a 989 amino acid protein that is a central component, a state variable, of the circadian biological clock. we have determined the sequence of all or part of this protein and surrounding regulatory regions from additional fungi representing three genera and report that there is distinct, preferential conservation of the frequency open reading frame (orf) as compared with non-coding sequences. within the coding region, many of the domain hallmarks of t ... | 1994 | 8194516 |
| evolution of the frequency (frq) clock locus in ascomycete fungi. | the frequency (frq) locus of neurospora crassa plays a key role in the organization of circadian rhythms. similar timing systems have been found in nearly all eukaryotes as well as some prokaryotes; thus, frq may be an excellent gene with which to conduct evolutionary studies. to investigate, we used the cloned frq locus from ascomycete fungi representing two classical taxonomic classes and three orders to examine two open questions in ascomycete evolution. class pyrenomycetidae is represented b ... | 1996 | 8896376 |
| analysis of frequency (frq) clock gene homologs: evidence for a helix-turn-helix transcription factor. | the frq gene plays a key role in the organization of the neurospora crassa circadian clock. our previous analysis of a c-terminal fragment of the putative frq protein suggested it is a nuclear transcription factor but did not identify a known dna-binding domain. if the hypothesis is correct that frq is a transcription factor, sequences consistent with this function should be conserved in distantly related species. to investigate, we have cloned frq homologs from other filamentous fungi including ... | 1997 | 9037100 |
| inherited and environmentally induced differences in mutation frequencies between wild strains of sordaria fimicola from "evolution canyon". | we have studied whether there is natural genetic variation for mutation frequencies, and whether any such variation is environment-related. mutation frequencies differed significantly between wild strains of the fungus sordaria fimicola isolated from a harsher or a milder microscale environment in "evolution canyon," israel. strains from the harsher, drier, south-facing slope had higher frequencies of new spontaneous mutations and of accumulated mutations than strains from the milder, lusher, no ... | 1998 | 9584088 |
| first radiobiological results of ldef-1 experiment a0015 with arabidopsis seed embryos and sordaria fungus spores. | this article highlights the first results of investigations on the general vitality and damage endpoints caused by cosmic ionizing radiation in dry, dormant plant seeds of the crucifer plant arabidopsis thaliana (l.) heynh. and the ascomycete sordaria fimicola after 69 month stay in space. wild-type and mutant gene marker lines were included in free flyer biostack containers and exposed on earth and side tray of the ldef-1 satellite. the damage in biological endpoints observed in the seeds inc ... | 1994 | 11539984 |
| inherited differences in crossing over and gene conversion frequencies between wild strains of sordaria fimicola from "evolution canyon". | recombination generates new combinations of existing genetic variation and therefore may be important in adaptation and evolution. we investigated whether there was natural genetic variation for recombination frequencies and whether any such variation was environment related and possibly adaptive. crossing over and gene conversion frequencies often differed significantly in a consistent direction between wild strains of the fungus sordaria fimicola isolated from a harsher or a milder microscale ... | 2001 | 11779798 |
| the relative ability of fungi from sphagnum fuscum to decompose selected carbon substrates. | nine species from a suite of 55 microfungi isolated from living and decomposing sphagnum fuscum were selected for studies of in vitro decomposition of tannic acid, cellulose, and starch. in vitro decomposition of s. fuscum plants and spruce wood chips was also examined. oidiodendron maius and oidiodendron scytaloides degraded tannic acid, giving a positive reaction for polyphenol oxidases. most taxa degraded cellulose and starch via the synthesis of cellulases and amylase, respectively. mass los ... | 2002 | 11989764 |
| transreplication and crossing over in sordaria fimicola. | a study of the segregation of markers closely linked to the gray ascospore color locus in sordaria fimicola reveals that there is a high incidence of crossing over very near the locus when it transreplicates, which is much more pronounced in 5:3 than in 6:2 asci. also, a single 7:1 and several aberrant 4:4 asci are described. at a different spore color locus, transreplication yields only 6:2 ratios, while other spore color loci fail to transreplicate altogether | 1961 | 13756458 |
| self-sterile auxotrophs and their relation to heterothallism in sordaria fimicola. | eighty morphological mutants in the homothallic fungus sordaria fimicola were tested on liquid minimal medium for nutritional requirements. five had nutritional requirements, one for adenine, three for arginine, and one for lysine. all five were from among the eighty single gene mutants that were also partially or completely self-sterile. nutritional requirements and centromere-locus intervals provide better criteria than morphological characters for selecting self-sterile mutants at complex loc ... | 1964 | 14172625 |
| radio-protective chemicals and genetic recombination of sordaria fimicola. | | 1964 | 14190058 |
| some coprophilous fungi from kenya. | the occurrence of coprophilous fungi on samples of antelope, buffalo, zebra and hippopotamus dung collected from the marula estates in kenya was recorded. a hundred and seventy three fungal isolates representative of 40 genera and 59 species were identified. among these species, 23 were ascomycetes (39%), 30 deuteromycetes (50.8%), 5 zygomycetes (8.5%) and 1 basidiomycete (1.7%). ascobolus immersus, coprotus niveus, iodophanus carneus, lasiobolus lasioboloides, podospora anserina, p. australis a ... | 1998 | 16284849 |
| interference competition among coprophilous fungi: production of (+)-isoepoxydon by poronia punctata. | (+)-isoepoxydon has been established as the major causative agent of interference competition between poronia punctata (nrrl 6457), a late fungal colonist of cattle dung, and two early-occurring dung colonists, ascobolus furfuraceus (nrrl 6460) and sordaria fimicola (nrrl 6459). this compound was isolated from ethyl acetate extracts of liquid cultures of p. punctata by silica gel chromatography and identified by mass spectrometry and proton and carbon nuclear magnetic resonance spectroscopy. the ... | 1988 | 16347607 |
| aberrant tetrads in sordaria fimicola. | | 1959 | 16590433 |
| the induction of balanced heterothallism in sordaria fimicola. | | 1962 | 16590909 |
| selective effects of purine and pyrimidine analogues and of respiratory inhibitors on perithecial development and branching in sordaria. | the initiation of perithecia in the homothallic ascomycete sordaria fimicola was completely suppressed, without seriously inhibiting vegetative growth, by growing the fungus on an agar medium containing one of the following additions: 1) 1 mum 5-fluorouracil, 2) 10 to 100 mum 6-azauracil, 8-azaguanine or 8-azaadenine, 3) 50 to 500 mum cyanide or azide, 4) 5% (w/v) casein hydrolysate. in contrast to the selective activity of the analogues of 3 rna bases, whose inhibition could be reversed by the ... | 1967 | 16656614 |
| absence of interference in association with gene conversion in sordaria fimicola, and presence of interference in association with ordinary recombination. | from the analysis of large samples of gene conversion asci in the g locus of sordaria fimicola, it was found that neither the conversion event itself nor conversion-associated recombination of flanking markers cause either chiasma or chromatid interference with crossing over in a neighboring interval. the presence of more than one kind of crossover event, one causing interference the other not, is considered. the existence of two kinds of gene loci, one of single-cistron composition and the othe ... | 1978 | 17176535 |
| headspace sorptive extraction and gc-tofms for the identification of volatile fungal metabolites. | volatiles from fungi cultivated in petri dishes were collected by a simple headspace polydimethylsiloxane (pdms) sorptive extraction technique (hsse), thermally desorbed into a gas chromatographic capillary column and detected and identified by gas chromatography-time-of-flight mass spectrometry (gc-tofms). the method was used to compare metabolite profiles of seven species of fungi grown on two types of sterile agars - potato dextrose and sabouraud dextrose. three species from the genus penicil ... | 2008 | 18625272 |
| the effects of biotin upon the formation and development of perithecia, asci and ascospores by sordaria fimicola ces. and de not. | | 1947 | 20249517 |
| the influence of ph and certain growth factors on mycelial growth and perithecial formation by sordaria fimicola. | | 1947 | 20295179 |