| [activities of some yeast flavogenic enzymes in situ]. | effects of digitonin, dimethylsulfoxide and protamine sulfate on yeast pichia guilliermondii were studied in order to produce cells with increased permeability and possessing the gtp-cyclohydrolase, riboflavinsynthetase and riboflavinkinase activities. the digitonin-treated cells exhibited a higher cyclohydrolase activity than the cell-free extracts; the activities of riboflavinsynthetase and riboflavinkinase in the cells and cell-free extracts were found to be similar. treatment of cells with d ... | 1977 | 199288 |
| the significance of candida pulcherrima findings in human clinical specimens. | from 1971 to 1976 the author examined by cultivation on sabouraud glucose agar, 1720 specimens of the most varied materials--skin scales, nails, hair, beard, sputum--obtained from patients suffering from skin problems. in no one single case did he find candida pulcherrima. however, since 1977 he has found c. pulcherrima to be on an increase. from 1977 to 1984 in 4644 specimens c. pulcherrima was found in 44 cases. 38 strains, i.e. 86%, were isolated from patients with clinical diagnosis of mycot ... | 1989 | 2615783 |
| [variety of effects of vitamins on yeast cells in aerobic conditions]. | the effect of thiamine and biotin on the processes of cell division, assimilation of glucose, and accumulation of the biomass and nitrogen in the cells was studied with the candida yeast. the action of the vitamins depended on the source of nitrogen. in some strains, asparagine can substitute for biotin. biotin has different effect on the production of gamma-aminobutyric acid in candida pulcherrima, c. guilliermondii. c. tropicalis k3-10. high concentrations of arginine were found in c. guillie ... | 1975 | 1214604 |
| [growth substance requirements in heterothallic yeast cultures]. | vitamin heterotrophy was studied among the following new yeast genera: leucosporidium, rhodosporidium and filobasidium. the requirement in growth factors of various crossing types was compared among these genera and ascomycetens yeast cultures saccharomycopsis lipolytica and metschnikowia pulcherrima. various crossing types of filobasidium capsuligenum were found to differ in their requirement in growth factors. various crossing types of rhodosporidium and saccharomycopsis lipolytica were stimul ... | 1976 | 1004264 |
| [yeast fungus infection caused by long-term parenteral nutrition at home. detection of candida pulcherrima (lindner) windisch as the pathogenic agent]. | | 1985 | 3935554 |
| [rate of reduction of certain pentoses by extracts from candida pulcherrima yeast]. | | 1968 | 4318330 |
| two unusual organisms, aspergillus terreus and metschnikowia pulcherrima, associated with the lung disease of ankylosing spondylitis. | | 1972 | 4628429 |
| [metabolic products of microorganisms. 124. incorporation of l-leucine in cyclo-l-leucyl-l-leucyl, the intermediate in the biosynthesis of pulcherriminic acid, in cell-free extracts from candida pulcherrima (author's transl)]. | | 1973 | 4778264 |
| the antigenic relationship between metschnikowia pulcherrima and the salmonella c1 and f group o antigens. | | 1973 | 4791974 |
| [d-ribokinase of the yeast candida pulcherrima (metschnikowia pulcherrima)]. | | 1972 | 5084507 |
| [pentoses and pentitols as sources of carbon and energy for candida pulcherrima]. | | 1970 | 5497265 |
| synonymy of metschnikowia pulcherrima and torulopsis burgeffiana. | torulopsis burgeffiana is placed in the genus metschnikowia because it has been shown to have a perfect stage corresponding with this genus. due to the characteristic shape of the ascus and ascospores, t. burgeffiana is to be considered a synonym of m. pulcherrima. the physiological differences which exist between strains are not sufficiently important to maintain a separate species. | 1971 | 5541542 |
| [the repeated isolation of candida pulcherrima (lindner) windisch from blood cultures of a patient on parenteral nutrition]. | | 1986 | 3084972 |
| [yeast flora of plant rhizosphere and phyllosphere]. | the species belonging to the genera cryptococcus and hansenula with saturnian spores predominate in the rhizosphere of agricultural plants. the sporiferous strains of debaryomyces, hanseniaspora apiculata, metschnikowia pulcherrima, and asporogenic candida krusei and trichosporon cutaneum prevail in the rhizosphere of wild plants. candida krusei and trichosporon cutaneum prevail in the rhizosphere of wild plants. the cultures of rhodotorula, candida krusei and metschnikowia pulcherrima are typic ... | 1975 | 1226148 |
| purification and properties of extracellular carboxyl proteinase secreted by candida pulcherrima. | an extracellular proteinase secreted by candida pulcherrima ksy 188-5 was purified about 60-fold to electrophoretical homogeneity from its culture supernatant, by ammonium sulfate fractionation, anion-exchange chromatography, and gel filtration. the proteinase had a molecular weight of approximately 36,500 and an isoelectric point of ph 4.7. the enzyme had an optimum ph of around 2.5-3.5 for activity and 3.0-5.0 for stability. the optimum temperature was around 45 degrees c at ph 3.0. the enzyme ... | 1995 | 7766170 |
| [the relation of candida pulcherrima and laspeyresia pomonella]. | | 1988 | 3185610 |
| aryl monooxygenase: a detoxifying enzyme from candida pulcherrima mcmy2. | characterization of partially purified aryl monooxygenase (1:14:14:1) from candida pulcherrima mcmy2 was achieved using standard purification protocol. the molecular weight of the enzyme was 110 kda and contained 3 subunits with pi 5.7, 7.4 and 7.6. the activity seemed to be related with pulcherrimin. the optimum ph and temperature for enzyme activity were 6.8 and 30 degrees c respectively. activity was not substrate specific and fe3+ fad and nadh+ enhanced the activity substantially. | 1996 | 9219440 |
| influence of temperature, ph and water activity on "in vitro" inhibition of penicillium glabrum (wehmer) westling by yeasts. | four different yeast species (metschnikowia pulcherrima, saccharomycopsis vini, kluyveromyces marxianus, cryptococcus albidus), isolated from surface of grapes, were evaluated for biocontrol potential against penicillium glabrum. in order to investigate the influence of temperature, ph, water activity and yeast cell concentration on penicillium glabrum inhibition, the individual effects and the interaction of these factors were analyzed by means of a central composite design (ccd). all yeast spe ... | 1998 | 9760746 |
| infection of an intravenous port system with metschnikowia pulcherrima pitt et miller. | a patient with short bowel syndrome as a consequence of multiple intestinal resections for crohn's disease, had a port system implanted to improve her nutritional status. one year later she presented with fever, weakness and nighttime sweating. metschnikowia pulcherrima pitt et miller was grown in blood cultures from the port system. after antifungal chemotherapy using fluconazole and removal of the implant, the patient's condition improved markedly and her fever and sweating disappeared. we con ... | 1998 | 9916468 |
| typing of non-saccharomyces yeasts with enzymatic activities of interest in wine-making. | enzymatic activity of potential interest in wine-making was studied for 182 non-saccharomyces yeasts isolated from musts before and at the onset of fermentation at wine cellars operating under the la mancha appellation of origin in spain. tests were carried out on plates containing differential substrates appropriate for each case (casein, gelatin, polygalacturonic acid, and arbutin) to determine whether each of the isolates exhibited proteolytic, polygalacturonase, and beta-glucosidase activiti ... | 2000 | 10946836 |
| killer behaviour in wild wine yeasts associated with merlot and malbec type musts spontaneously fermented from northwestern patagonia (argentina). | the occurrence of killer wine yeasts in comahue region (patagonia, argentina) was studied. wild wine yeasts were isolated from spontaneously fermenting merlot and malbec type musts. out of 135 isolates analyzed 37% were sensitive to some well characterized killer toxins as k1-k10 and did not show killer activity (sensitive phenotype, s), 21% showed neutral phenotype (n) and 42% demonstrated killer activity (killer phenotype, k). all but two killer strains, identified as candida pulcherrima and k ... | 2001 | 11441458 |
| fermentability of grape must after inhibition with dimethyl dicarbonate (dmdc). | dimethyl dicarbonate (dmdc) was added to grape must and to synthetic media and results showed that, at 20 degrees c, 150 mg.l(-)(1) dmdc completely inhibited the fermentation of a grape must that was previously inoculated with 10(6) cells.ml(-)(1) saccharomyces bayanus and saccharomyces uvarum. brettanomyces intermedius, candida guilliermondii, hansenula jadinii, hansenula petersonii, kloeckera apiculata, pichia membranaefaciens, and saccharomyces cerevisiae were inhibited by 250 mg.l(-)(1). can ... | 2002 | 12236685 |
| candida kunwiensis sp. nov., a yeast associated with flowers and bumblebees. | a novel asexual ascomycetous yeast, candida kunwiensis (sg99-26t = kctc 17041t = cbs 9067t), was isolated from sweet potato (ipomoea batatas) flowers in korea and from the body surface of pollinating bumblebees in germany. comparative analysis of the d1/d2 domain of 26s rdna of all available sequences for ascomycetous yeasts showed that the novel species was phylogenetically related to the genus metschnikowia, but the sequence similarity was low. morphologically and physiologically, c. kunwiensi ... | 2003 | 12656196 |
| the species-specific ratios of 2,3-butanediol and acetoin isomers as a tool to evaluate wine yeast performance. | the isomers of 2,3-butanediol [r,r; s,s; r,s (meso-form)] and of acetoin (r,s) were determined in laboratory wine fermentations carried out by 50 yeast strains, 10 for each of the following species, saccharomyces cerevisiae, kloeckera apiculata, candida stellata, metschnikowia pulcherrima and brettanomyces bruxellensis, in order to evaluate if such parameters might be used to differentiate wines obtained with different yeast species. according to analysis of variance (anova), the strains of the ... | 2003 | 12892931 |
| the diversity of yeasts in the agricultural soil. | one hundred and eleven yeast strains were isolated from 60 agricultural soil samples. the samples were taken from four various fields located in the southwest of slovakia. cryptococcus laurentii, candida maltosa, metschnikowia pulcherrima, and sporobolomyces salmonicolor were the predominant species in the samples collected from all four types of fields. these species represented 78.4-86.6% of the total yeast counts.the results obtained enabled comparisons to be made between forest and agricultu ... | 2003 | 12964187 |
| [adaptation of yeasts to pentoses. on the adaptation of candida pulcherrima to d-xylose]. | | 1961 | 14453883 |
| [variations in the content of non-volatile aliphatic acids produced by yeasts (candida robusta, candida pulcherrima and candida albicans) in various forms of resting cells]. | | 1962 | 14460212 |
| [variations in the utilization of glucose by non-sporogenous yeasts (candida robusta, candida pulcherrima and candida albicans) with different morphological features of resting cells]. | | 1962 | 14460213 |
| a mixed culture of propionibacterium jensenii and lactobacillus paracasei subsp. paracasei inhibits food spoilage yeasts. | screening for antimicrobial features of 197 propionibacteria and tests with several antifungal lactobacilli led to the development of three protective cultures containing propionibacterium jensenii sm11 and lactobacillus paracasei subsp. paracasei strain sm20, sm29 or sm63. these cultures showed inhibitory activities (up to 5 orders of magnitude) against yeasts in dairy products such as yoghurt or cheese surface at refrigerator temperatures (6 degrees c) without an influence on the quality prope ... | 2004 | 15046312 |
| study of the dermatophytes in dogs and the risk of human infection. | the infiltrative growth of fungi that multiplies in the tissues (dermatophytes, candida, agents of visceral mycoses) is the generally known proof of "active" functioning of these organisms. the relative importance of fungi as inducers of human and animal diseases grows constantly. | 2003 | 15168865 |
| [arthrobacter globiformis - a new yeast-lysing bacterium]. | a bacterium capable to lyse viable yeast cells was isolated from compost enriched with baker's yeast cells and was identified as arthrobacter globiformis. the yeast lytic enzyme complex produced by shaking culture was precipitated with ammonium sulfate, dialyzed and lyophilized. the crude residue contained beta-glucanase and alpha-mannanase, yet not chitinase. optimale carbon sources in the culture medium for a high enzyme synthesis were 0.5% beta-glucan for the production of beta-glucanase, res ... | 1982 | 7157841 |
| differentiation between atypical isolates of candida lusitaniae and candida pulcherrima by determination of mating type. | we report on five clinical isolates routinely identified as candida lusitaniae that the id 32c system was unable to discriminate from the closely related species candida pulcherrima. when additional tests did not allow accurate identification, the less usual mating type test identified all of them as clavispora lusitaniae. mating type testing appears to be a valuable tool for assessing the true incidence of this emerging non-albicans candida species. | 2005 | 15750124 |
| chitosan inactivates spoilage yeasts but enhances survival of escherichia coli o157:h7 in apple juice. | to develop new measures for controlling both spoilage and pathogenic micro-organisms in unpasteurized apple juice using chitosan. | 2005 | 15752333 |
| dynamics of indigenous yeast populations during spontaneous fermentation of wines from mendoza, argentina. | fermentation of wine is a complex microbial reaction, which involves the sequential development of various species of yeasts and lactic acid bacteria. of these, yeasts are the main group responsible for alcoholic fermentation. the aim of this work was to study, under industrial conditions, the evolution of yeast populations and to describe the individual evolution of the most important yeasts during three spontaneous fermentations of malbec musts from argentina. this work shows the significant p ... | 2005 | 15808358 |
| yeasts associated to malbec grape berries from mendoza, argentina. | the aims of this work were to evaluate different pre-isolation treatments applied to complete yeast extraction from grapes and to identify the yeast microflora associated to malbec grapes from two vineyards located in mendoza, argentina. | 2005 | 15836474 |
| analysis of yeast isolates related to metschnikowia pulcherrima using the partial sequences of the large subunit rdna and the actin gene; description of metschnikowia andauensis sp. nov. | thirty-two yeast isolates were cultured from guts or excrements of three different pests of corn or from the stem of healthy corn. the strains were analyzed using msp-pcr (micro/minisatellite-primed polymerase chain reaction), sequences of the d1/d2 region of the large subunit rdna and a 979 bp long part of the actin gene (act-1). they seem to belong to three groups that are all sister groups of metschnikowia pulcherrima, m. fructicola and m. chrysoperlae. a new species, metschnikowia andauensis ... | 2005 | 16261861 |
| phylogeny of metschnikowia species estimated from partial rrna sequences. | phylogenetic relationships of species assigned to the genus metschnikowia were estimated from the extents of divergence among partial sequences of rrna. the data suggest that the aquatic species (metschnikowia australis, metschnikowia bicuspidata, metschnikowia krissii, and metschnikowia zobellii) and the terrestrial species (metschnikowia hawaiiensis, metschnikowia lunata, metschnikowia pulcherrima, and metschnikowia reukaufii) form two groups within the genus. m. lunata and m. hawaiiensis are ... | 1993 | 8494745 |
| analysis of yeast flora associated with grape sour rot and of the chemical disease markers. | the frequency and the density of the species associated with grape sour rot in different cultivars were determined. the most frequent species in the rotten grapes, candida krusei, kloeckera apiculata, and metschnikowia pulcherrima, and a less frequent species, issatchenkia occidentalis, when inoculated with saccharomycopsis crataegensis were able to induce in vitro the symptoms of the disease. the gas chromatographic determination of the volatile compounds in the headspace was used to evaluate t ... | 1987 | 16347305 |
| characterization of some yeasts isolated from foods by traditional and molecular tests. | in this study, 22 yeast strains isolated from foods were characterized by traditional and molecular techniques. with the help of traditional identification tests, yeast strains were grouped in 12 species belonging to 11 genera as follows: candida parapsilosis, rhodotorula mucilaginosa, debaryomyces hansenii, cryptococcus humicolus, cryptococcus albidus, aureobasidium spp., hanseniaspora valbyensis, metschnikowia pulcherrima, lachancea thermotolerans, pichia anomala, geotrichum candidum and yarro ... | 2006 | 16388865 |
| biosynthesis of pulcherriminic acid. | 1. candida pulcherrima was grown on a complex medium to which various compounds had been added to determine their effect on the biosynthesis of pulcherriminic acid. most of the pulcherriminic acid synthesized by c. pulcherrima prl2019 was derived from the l-[1-(14)c]leucine added to the medium. 2. the cyclic dipeptide of l-leucine (cyclo-l-leucyl-l-leucyl) was shown, by trapping experiments involving cycloleucyl-leucyl isomers, to be synthesized by strain prl2019. cyclo-l-leucyl-l-leucyl was der ... | 1965 | 5837792 |
| biocontrol of the food-borne pathogens listeria monocytogenes and salmonella enterica serovar poona on fresh-cut apples with naturally occurring bacterial and yeast antagonists. | fresh-cut apples contaminated with either listeria monocytogenes or salmonella enterica serovar poona, using strains implicated in outbreaks, were treated with one of 17 antagonists originally selected for their ability to inhibit fungal postharvest decay on fruit. while most of the antagonists increased the growth of the food-borne pathogens, four of them, including gluconobacter asaii (t1-d1), a candida sp. (t4-e4), discosphaerina fagi (st1-c9), and metschnikowia pulcherrima (t1-e2), proved ef ... | 2006 | 16461659 |
| growth of natural yeast flora during the fermentation of inoculated wines. | the growth of yeasts that occur naturally in grape juice was quantitatively examined during the fermentation of four wines that had been inoculated with saccharomyces cerevisiae. although s. cerevisiae dominated the wine fermentations, there was significant growth of the natural species kloeckera apiculata, candida stellata, candida colliculosa, candida pulcherrima, and hansenula anomala. | 1985 | 16346894 |
| effects of penconazole on two yeast strains: growth kinetics and molecular studies. | the aim of this study consisted to evaluate the impact of a pesticide (penconazole) on the growth kinetics and genotoxicity on two yeast strains (saccharomyces cerevisiae and metschnikowia pulcherrima). when the penconazole was added at different phases of the growth of m. pulcherrima, no effect was noticed on the kinetics of yeast growth but dna adducts were observed when penconazole was added in the exponential phase. increasing doses (1-15 maximum residue limit) of the pesticide added at the ... | 2006 | 16729354 |
| dynamics and characterization of yeasts during natural fermentation of italian sausages. | a multiphasic approach was used to investigate the yeast ecology in italian fermented sausages. culture-dependent and -independent methods were applied to identify the yeast species during the maturation process and to characterize the numerically dominant species. plating analysis and subsequent molecular identification of the isolates highlighted the dominance of debaryomyces hansenii, but at least other three yeast species -candida zeylanoides, pichia triangularis and metschnikowia pulcherrim ... | 2006 | 16879421 |
| moulds and yeasts in fruit salads and fruit juices. | thirty-eight fruit salad samples including cantaloupe, citrus fruits, honeydew, pineapple, cut strawberries and mixed fruit salads, and 65 pasteurized fruit juice samples (apple, carrot, grapefruit, grape and orange juices, apple cider, and soy milk) were purchased from local supermarkets in the washington, dc area and tested for fungal contamination. the majority of fruit salad samples (97%) were contaminated with yeasts at levels ranging from <2.0 to 9.72 log10 of colony forming units per gram ... | 2006 | 16943069 |
| yeast species associated with the spontaneous fermentation of cider. | this paper reports the influence of cider-making technology (pneumatic and traditional pressing) on the dynamics of wild yeast populations. yeast colonies isolated from apple juice before and throughout fermentation at a cider cellar of asturias (spain), during two consecutive years were studied. the yeast strains were identified by restriction fragment length polymorphism analysis of the 5.8s rrna gene and the two flanking internal transcribed sequences (its). the musts obtained by pneumatic pr ... | 2007 | 16943091 |
| metschnikowia sinensis sp. nov., metschnikowia zizyphicola sp. nov. and metschnikowia shanxiensis sp. nov., novel yeast species from jujube fruit. | eight yeast strains were isolated from jujube fruit surfaces collected in shanxi and shandong provinces, china. all eight strains produced needle-shaped ascospores under suitable conditions. three separate groups, representing three novel species in the genus metschnikowia, were recognized by sequence comparisons of the 26s rdna d1/d2 domain and internal transcribed spacer (its) region. the names metschnikowia sinensis sp. nov. (type strain xy103(t)=as 2.3110(t)=cbs 10357(t)), metschnikowia zizy ... | 2006 | 16957129 |
| [moulds and yeasts in bottled water and soft drinks]. | some damaged cartons of soft drinks and carbonated water were analyzed to detect the microorganisms that caused the damage. the contaminants of sugar used in the production of one of the drinks were also studied. the methods of déak & beuchat and pitt & hocking were used for the identification of yeasts and moulds, respectively. the agents of the spoilage of soft drinks were debaryomyces hansenii, debaryomyces polymorphus, galactomyces geotrichum, metschnikowia pulcherrima, mucor circinelloides, ... | 2006 | 17037258 |
| interaction between glomus mosseae and soil yeasts on growth and nutrition of cowpea. | a glass house experiment was conducted to study the interaction between the mycorrhizal fungus, glomus mosseae and six soil yeasts (rhodotorula mucilaginosa, metschnikowia pulcherrima, trichosporon cutaneum var. cutaneum, saccharomyces cerevisiae, cryptococcus laurentii, debaryomyces occidentalis var. occidentalis), and their effect on growth and nutrition of cowpea. all the yeasts had a synergistic interaction with the mycorrhizal fungus and dual inoculation improved plant growth compared to si ... | 2008 | 17324563 |
| isolation of epiphytic yeasts with potential for biocontrol of aspergillus carbonarius and a. niger on grape. | antagonistic yeasts were isolated from the epiphytic flora associated with grape berries cv. negroamaro and identified at species level using molecular methods. a total of 144 yeast isolates were tested in a preliminary screening on agar to select isolates showing a killer activity against aspergillus carbonarius and a. niger, the main species responsible for the accumulation of ochratoxin a in grape. twenty-eight yeast isolates were selected for their inhibitory effects on the above fungal spec ... | 2006 | 16443300 |
| metschnikowia chrysoperlae sp. nov., candida picachoensis sp. nov. and candida pimensis sp. nov., isolated from the green lacewings chrysoperla comanche and chrysoperla carnea (neuroptera: chrysopidae). | fourteen yeast isolates comprising three taxa were cultured from digestive tracts of adult chrysoperla species (neuroptera: chrysopidae) and their eggs. the yeast taxa were distinguished based on an estimated molecular phylogeny, dna sequences and traditional taxonomic criteria. the new yeasts are closely related to metschnikowia pulcherrima but are sufficiently distinguished by sequence comparison of rrna gene sequences to consider them as novel species. here, three novel species are described ... | 2004 | 15388758 |
| yeasts colonizing the leaf surfaces. | the yeasts were isolated from the leaf surfaces of ten species of trees. the study site was a forest park (zelezná studnicka) of the small carpathians mountain range. one hundred and thirty seven yeast strains belonging to 13 genera were isolated from 320 samples of leaves and needles. seventeen yeast species were isolated, but only seven occurred regularly: aureobasidium pullulans, cryptococcus laurentii, pichia anomala, metschnikowia pulcherrima, saccharomyces sp., lachancea thermotolerans, an ... | 2007 | 17645279 |
| efficacy of chromogenic candida agar for isolation and presumptive identification of pathogenic yeast species. | chromogenic candida agar is a novel differential culture medium that is claimed to facilitate isolation and identification of candida albicans, candida tropicalis and candida krusei. the performance of this medium was evaluated for presumptive identification of 521 yeast strains, representing 23 different species, for detection of specimens containing yeast mixtures, and for direct isolation of yeast from blood cultures. all yeasts grew well on the medium following a 48-h incubation period at 37 ... | 2008 | 17986267 |
| dna microarray based on arrayed-primer extension technique for identification of pathogenic fungi responsible for invasive and superficial mycoses. | an oligonucleotide microarray based on the arrayed-primer extension (apex) technique has been developed to simultaneously identify pathogenic fungi frequently isolated from invasive and superficial infections. species-specific oligonucleotide probes complementary to the internal transcribed spacer 1 and 2 (its1 and its2) region were designed for 24 species belonging to 10 genera, including candida species (candida albicans, candida dubliniensis, candida famata, candida glabrata, candida tropical ... | 2008 | 18160452 |
| one-step purification and characterization of an intracellular beta-glucosidase from metschnikowia pulcherrima. | a collection of 60 non-saccharomyces yeasts isolated from grape musts in uruguayan vineyards was screened for beta-glucosidase activity and metschnikowia pulcherrima was the best source of this enzyme activity. its major beta-glucosidase was successfully purified to homogeneity by ion-exchange chromatography on amino-agarose gel. the enzyme exhibited an optimum catalytic activity at 50 degrees c and ph 4.5 and was active against (1 --> 4)-beta and (1 --> 2)-beta glycosidic linkages. in spite of ... | 2008 | 18414804 |
| killer activity of yeasts isolated from natural environments against some medically important candida species. | twenty-five yeast cultures, mainly of human origin, belonging to four pathogenic yeast species--candida albicans, candida krusei, candida parapsilosis, and candida tropicalis were tested for their sensitivity to ten basidiomycetous and eleven ascomycetous yeast species isolated from the water and soil environments and from tree leaves. the best killer activity among basidiomycetous species was exhibited by rhodotorula glutinis, and r. mucilaginosa. the other carotenoid producing species cystofil ... | 2007 | 17419188 |
| characterization of low-molecular-weight antiyeast metabolites produced by a food-protective lactobacillus-propionibacterium coculture. | we developed a ph-controlled batch fermentation process with separately immobilized cells of the protective coculture of lactobacillus paracasei subsp. paracasei sm20 and propionibacterium jensenii sm11 in supplemented whey permeate medium yielding cell-free supernatants with high antiyeast activity against candida pulcherrima and rhodotorula mucilaginosa. the antiyeast compounds were resistant to proteinase k and pronase e treatments and showed high heat resistance (121 degrees c for 15 min). d ... | 2008 | 19244902 |
| [metschnikowia pulcherrima, antifungal biocontrol agent taxonomic classification, morpho-physiological and genetic aspects, antifungal action]. | | 2007 | 19326726 |
| use of aflp for differentiation of metschnikowia pulcherrima strains for postharvest disease biological control. | metschnikowia pulcherrima occurs naturally on fruits, buds and floral parts of apple trees. some strains are effective as biocontrol agents against postharvest decay of apples and other fruits. the usefulness of the amplified fragment length polymorphism (aflp) technique was evaluated for the genetic analysis of 26 strains of m. pulcherrima, isolated from different sources in different geographical regions. with six aflp primer pairs, 729 polymorphic bands were scored. the technique showed a hig ... | 2008 | 17428647 |
| pulcherrimin, the pigment of candida pulcherrima. | | 1953 | 16589308 |
| differential response of pichia guilliermondii spoilage isolates to biological and physico-chemical factors prevailing in patagonian wine fermentations. | pichia guilliermondii can produce volatile phenols in the initial stages of wine fermentation; however, its response to different wine stress conditions has been poorly studied. in this work, we present an analysis of the response of 23 p. guilliermondii indigenous isolates to physical and chemical wine stress factors and to indigenous wine killer yeasts. principal coordinates analysis (pcoa), based on data obtained from response patterns, was carried out to evaluate the relationships among the ... | 2009 | 19767852 |
| the effect of debina grapevine indigenous yeast strains of metschnikowia and saccharomyces on wine flavour. | the spontaneous alcoholic fermentation of grape must is a complex microbiological process involving a large number of various yeast species, to which the flavour of every traditional wine is largely attributed. whilst saccharomyces cerevisiae is primarily responsible for the conversion of sugar to alcohol, the activities of various non-saccharomyces species enhance wine flavour. in this study, indigenous yeast strains belonging to metschnikowia pulcherrima var. zitsae as well as saccharomyces ce ... | 2010 | 19859752 |
| yeast species associated with wine grapes in china. | having more information on the yeast ecology of grapes is important for wine-makers to produce wine with high quality and typical attributes. china is a significant wine-consuming country and is becoming a serious wine-producer, but little has been reported about the yeast ecology of local ecosystems. this study provides the first step towards the exploitation of the yeast wealth in china's vine-growing regions. the aim of this study was to investigate the yeast population density and diversity ... | 2010 | 20116124 |
| influence of candida pulcherrima patagonian strain on alcoholic fermentation behaviour and wine aroma. | the use of selected saccharomyces and non-saccharomyces strains as mixed starters in winemaking would have advantages over the traditional spontaneous fermentation, producing wines with predictable and desirable characteristics. the aim of this study was to evaluate the impact of metabolic interactions between patagonian indigenous saccharomyces cerevisiae mmf9 and beta-glucosidase producer candida pulcherrima v(6) strains on alcoholic fermentation behaviour and wine aroma three inoculation stra ... | 2010 | 20116878 |
| effect of culture media and ph on the biomass production and biocontrol efficacy of a metschnikowia pulcherrima strain to be used as a biofungicide for postharvest disease control. | few strains of metschnikowia pulcherrima (pitt) m.w. miller are under development for control of postharvest pathogens on fruit. a substrate was developed to optimize the biomass production of m. pulcherrima strain bio126. different complex nutrient sources, with or without ph control, were tested. growth in yeast extract provided at concentrations > or =30 g*l-1 yielded the highest biomass. the addition of two carbon sources, d-mannitol and l-sorbose, at 5 g*l-1 each, significantly improved yea ... | 2010 | 20237574 |
| genetic and phenotypic diversity of autochthonous cider yeasts in a cellar from asturias. | this paper analyses yeast diversity and dynamics during the production of asturian cider. yeasts were isolated from apple juice and at different stages of fermentation in a cellar in villaviciosa during two asturian cider-apple harvests. the species identified by its-rflp corresponded to hanseniaspora valbyensis, hanseniaspora uvarum, metschnikowia pulcherrima, pichia guilliermondii, candida parapsilosis, saccharomyces cerevisiae and saccharomyces bayanus/saccharomyces pastorianus/saccharomyces ... | 2010 | 20417399 |
| yeast heterogeneity during spontaneous fermentation of black conservolea olives in different brine solutions. | to assess the yeast community structure and dynamics during greek-style processing of natural black conservolea olives in different brine solutions. | 2010 | 20438554 |
| characterization of the yeast ecosystem in grape must and wine using real-time pcr. | the complex microbial ecosystem of grape must and wine harbours a wide diversity of yeast species. specific oligonucleotide primers for real-time quantitative pcr(qpcr) were designed to analyse several important non-saccharomyces yeasts (issatchenkia orientalis, metschnikowia pulcherrima, torulaspora delbrueckii, candida zemplinina and hanseniaspora spp.) and saccharomyces spp. in fresh wine must, during fermentation and in the finished wine. the specificity of all primer couples for their targe ... | 2010 | 20510771 |
| evaluation of fungal and yeast diversity in slovakian wine-related microbial communities. | since the yeast flora of slovakian enology has not previously been investigated by culture-independent methods, this approach was applied to two most common cultivars frankovka (red wine) and veltlin (white wine), and complemented by cultivation. model samples included grapes, initial must, middle fermenting must and must in the end-fermentation phase. the cultured isolates were characterized by length polymorphism of rdna spacer two region using fluorescence pcr and capillary electrophoresis (f ... | 2010 | 20556654 |
| effect of culture age, protectants, and initial cell concentration on viability of freeze-dried cells of metschnikowia pulcherrima. | the effect of freeze-drying using different lyoprotectants at different concentrations on the viability and biocontrol efficacy of metschnikowia pulcherrima was evaluated. the effects of initial yeast cell concentration and culture age on viability were also considered. yeast cells grown for 36 h were more resistant to freeze-drying than were 48 h cells. an initial concentration of 10⁸ cells·ml⁻¹ favoured the highest survival after freeze-drying. when maltose (25%, m/v) was used as protectant, a ... | 2010 | 20962903 |
| potential of two metschnikowia pulcherrima (yeast) strains for in vitro biodegradation of patulin. | patulin contamination of apple and other fruit-based foods and beverages is an important food safety issue, as consumption of these commodities throughout the world is great. studies are therefore necessary to reduce patulin levels to acceptable limits or undetectable levels to minimize toxicity. this study was undertaken to investigate the efficacy of two metschnikowia pulcherrima strains (mach1 and gs9) on biodegradation of patulin under in vitro conditions. these yeast strains were tested for ... | 2011 | 21219780 |
| optimization of killer assays for yeast selection protocols. | a new optimized semiquantitative yeast killer assay is reported for the first time. the killer activity of 36 yeast isolates belonging to three species, namely, metschnikowia pulcherrima, wickerhamomyces anomala and torulaspora delbrueckii, was tested with a view to potentially using these yeasts as biocontrol agents against the wine spoilage species pichia guilliermondii and pichia membranifaciens. the effectiveness of the classical streak-based (qualitative method) and the new semiquantitative ... | 2010 | 21229201 |
| in vitro antifungal activity of phenothiazines and their combination with amphotericin b against different candida species. | candidiosis is a mycosis that is currently increasingly affecting the population in consequence of its frequency and the severity of its complications, especially among immunocompromised hosts. in this work, the in vitro anticandidal activities of two phenothiazines (ptzs), chlorpromazine (cpz) and trifluoperazine (tfp), and their combinations with amphotericin b (amb) were tested against 12 different candida strains representing 12 species (candida albicans, candida glabrata, candida guillermon ... | 2011 | 21605196 |
| evaluation of chrom-pal medium for the isolation and direct identification of candida dubliniensis in primary cultures from the oral cavity. | candida albicans is the species most frequently isolated from oral specimens, but the recovery of other candida species such as candida dubliniensis is increasing. differentiation of c. dubliniensis from c. albicans requires special tests and both species are misidentified in some studies. chrom-pal (ch-p) is a novel chromogenic medium used in our laboratory for differentiation between c. albicans and c. dubliniensis on the basis of colony colour and morphology, and chlamydospore production. the ... | 2009 | 19608690 |
| fungaemia caused by candida pulcherrima. | although neonatal bloodstream infections may be caused by a variety of fungi, invasive fungaemia due to candida pulcherrima in a premature neonate has not been previously reported. we describe such a case in which antifungal susceptibility test data led to successful therapy. a colonized catheter used for parenteral nutrition is presumed to have been the main source of this persistent infection. | 2011 | 22149973 |
| Tandem repeat-tRNA (TRtRNA) PCR method for the molecular typing of non-Saccharomyces subspecies. | There is a worldwide trend to understand the impact of non-Saccharomyces yeast species on the process of winemaking. Although the predominant species at the end of the fermentation is Saccharomyces cerevisiae, several non-Saccharomyces species present during the first days of the process can produce and/or release aromas that improve the bouquet and complexity of the final wine. Since no genomic sequences are available for the predominant non-Saccharomyces species selected from grapes or musts ( ... | 2011 | 22113560 |
| influence of pesticides on yeasts colonizing leaves. | the effect of nine different pesticides on the growth of yeasts isolated from the leaves of fruit and forest trees was investigated. four insecticides (with the active ingredients: thiacloprid, deltamethrin, lambdacyhalothrin, and thiamethoxam) and five fungicides (with the effective substances: bitertanol, kresoxim-methyl, mancozeb, trifloxystrobin, and cupric oxychloride) were tested. the concentrations of chemicals were those recommended by the manufacturers for the spraying of trees. the yea ... | 2011 | 22351984 |
| isolation and characterization of new metschnikowia pulcherrima strains as producers of the antimicrobial pigment pulcherrimin. | metschnikowia pulcherrima is a highly effective biocontrol yeast due to its pigment pulcherrimin that accumulates in the cells and in the growth medium. three different strains of m. pulcherrima were isolated from local grapes. the yeast isolates were characterized on the basis of their biochemical, physiological and its1-5.8 s rdna-its2 region. based on the obtained results, the m. pulcherrima isolates were identified as new strains of m. pulcherrima. strong antagonistic activities of the m. pu ... | 2009 | 19678547 |
| biocontrol potential of metchnikowia pulcherrima strains against blue mold of apple. | abstract eight strains of metschnikowia pulcherrima isolated over a 4-year period from an unmanaged orchard and selected for their biocontrol activity against blue mold (caused by penicillium expansum) of apples were characterized phenotypically, genetically, and for their biocontrol potential against blue mold on apples. all strains grew well and only differed slightly in their growth in nutrient yeast dextrose broth medium at 1 degrees c after 216 h, but large differences occurred at 0 degrees ... | 2001 | 18943447 |
| influence of fenamidone, indoxacarb, pyraclostrobin, and deltamethrin on the population of natural yeast microflora during winemaking of two sardinian grape cultivars. | the influence of fenamidone ((s)-1-anilino-4-methyl-2-methylthio-4-phenylimidazolin-5-one), pyraclostrobin (methyl 2-[1-(4-chlorophenyl)pyrazol-3-yloxymethyl]-n-methoxycarbanilate), indoxacarb (methyl 7-chloro-2,5-dihydro-2-[[(methoxycarbonyl) [4- (trifluoromethoxy) phenyl] amino] carbonyl] indeno[1,2-e][1,3,4] oxadiazine-4a(3h)-carboxylate), and deltamethrin ([cyano-[3-(phenoxy)phenyl]methyl] 3-(2,2-dibromoethenyl)-2,2-dimethylcyclopropane-1-carboxylate) on spontaneous fermentation carried out ... | 2011 | 21726147 |