| purification and characterization of two lectins from caragana arborescens seeds. | a glycoprotein fraction with hemagglutinating activity was purified by affinity chromatography from seeds of the pea tree, caragana arborescens. subsequent fractionation resolved two components, which could be separated on a preparative scale using different affinity matrices. the major component binds to n-acetylgalactosamine coupled to sepharose 4b. it is a glycoprotein with high hemagglutinating activity. it is composed of two types of polypeptides, present in nonstoichiometric amounts, with ... | 1976 | 972148 |
| assignment of lectins specific for d-galactose or n-acetyl-d-galactosamine to two groups, based on their circular dichroism. | the circular dichroism (cd) spectra of thirteen lectins, most being specific for d-galactose or n-acetyl-d-galactosamine, were compared. two groupings are proposed on the basis of the cd in the near-ultraviolet region. group one comprises the lectins from arachis hypogaea, glycine max, phaseolus coccineus, p. lunatus, p. vulgaris, and vicia villosa, and group two comprises lectins from caragana arborescens, cytisus sessilifolius, dolichos biflorus, griffonia simplicifolia, and wisteria floribund ... | 1985 | 4016573 |
| induced polyploids in caragana arborescens lam. | | 1974 | 4453854 |
| lectin and immunohistochemical comparison of glycoconjugates in the conjunctiva of patients with and without exfoliation syndrome. | aims--the study was carried out to search for labelling similar to that of intraocular exfoliation material in the conjunctiva by light microscopy using lectin and immunohistochemistry. methods--ten formalin fixed and paraffin embedded conjunctival biopsy specimens both from patients with and without exfoliation syndrome were studied with a panel of 11 lectins and with three monoclonal antibodies to the hnk-1 carbohydrate epitope, all of which react with intraocular exfoliation material. results ... | 1995 | 7542024 |
| galactose-containing glycoconjugates of the ciliary body and lens in capsular glaucoma: a lectin histochemical study. | this study was carried out in order to obtain information on galactose-containing glycoconjugates in the lens and ciliary body of human eyes with capsular glaucoma and thus on the etiopathogenesis of exfoliation syndrome. | 1994 | 8001826 |
| segmental differentiation of permeability, protein glycosylation, and morphology of cultured bovine lung vascular endothelium. | the barrier function, surface biochemistry, and morphology of confluent monolayers of endothelial cells isolated from different segments of the bovine lung vasculature [microvessels (blmvec), vein (bpvec) and artery (bpaec)] were grown in culture and compared. a number of common cell surface proteins were identified along with two proteins of 46 and 48 kda found exclusively on bpvec. lectin affinity chromatography revealed multiple glycosylation differences. the lectins, arachis hypogaea (aha) a ... | 1994 | 8123001 |
| galactose-deficient iga1 in sera of iga nephropathy patients is present in complexes with igg. | iga1 proteins from sera of patients with iga nephropathy (igan) are galactosylated to a lesser degree than those from healthy controls. the increased reactivity of intact or de-sialylated serum iga1 with n-acetylgalactosamine (galnac)-specific lectins, helix aspersa (haa) and caragana arborescens (caa) and de-sialylated iga1 with helix pomatia (hpa) and bauhinia purpurea (bpa) indicated that the gal deficiency is in glycans located in the hinge region of iga1 molecules. de-sialylated iga from se ... | 1997 | 9264010 |
| [caragana arborescens lectin--a marker of myoepitheliocytes of salivary glands]. | submaxillary salivary gland preparations of a horse, a cow, a pig, a rabbit and white outbred rats were stained with peroxidase-labeled lectins of different carbohydrate specificity. it was found that n-acetyl-d-galactosamine-specific lectins (caragana arborescens lam., phaseolus unatus seeds, helix pomatia sails) bind selectively with myoepitheliocytes. best marker of the latter proved to be caragana arborescens lectin, consisting of two components which differ in their affinity to n-acetyl-d-g ... | 1999 | 10486811 |
| forssman pentasaccharide and polyvalent galbeta1-->4glcnac as major ligands with affinity for caragana arborescens agglutinin. | the binding properties of caragana arborescens agglutinin (caa, pea tree agglutinin) were studied by enzyme linked lectinosorbent assay (ellsa) and by inhibition of caa-glycan interaction. among glycoproteins (gps) tested, caa reacted strongly with asialo bird nest gp, asialo rat sublingual gp, human tamm-horsfall sd(a(+)) urinary gp (thgp) and asialo thgp that are rich in galnacalpha1-->, galnacbeta1--> and/or galbeta1-->4glcnac residues. caa also bound tightly with multi-valent galbeta1-->4glc ... | 1999 | 10606726 |
| [neoformation of cell nuclei in stems of caragana arborescens lam]. | | 1954 | 13162645 |