| aspergillus fumigatus and aspergillus niger in two potted ornamental plants, cactus (epiphyllum truncatum) and clivia (clivia miniata). biological and epidemiological aspects. | the presence and growth of aspergillus fumigatus and aspergillus niger in the soil of ornamental plants have been demonstrated. the ecological conditions in the soil of such plants as influenced by temperature, humidity, desiccation, fertilization and ventilation obviously influence such fungal growth. the epidemiological significance of these findings is of interest with a view to the present efforts to control aspergillosis in the environment of susceptible persons. observations of a preferent ... | 1978 | 375093 |
| preliminary isolated organ studies using an aqueous extract of clivia miniata leaves. | an infusion of clivia miniata leaves is a traditional medicine taken by black south african women to induce or augment labour. a boiling water extract of c. miniata leaves was studied for its pharmacological effects on rat uterus and ileum. the extract caused concentration-dependent contractions in both the uterus and ileum, but at a fraction of the intrinsic activity of acetylcholine. when the organs were pretreated with the extract before the cumulative addition of either oxytocin or acetylcho ... | 1989 | 2615439 |
| coupling sfe to uterotonic bioassay: an on-line approach to analysing medicinal plants. | supercritical fluid extraction has been directly coupled on-line to a uterotonic bioassay, using guinea pig uterine smooth muscle in vitro. this technique was developed for the detection of uterotonic compounds present in medicinal plants used during pregnancy to induce or augment labour. the direct passage of co2 into the muscle chamber led to adiabatic cooling of the physiological fluid and inhibition of muscle contraction. this was alleviated by the construction of a co2 reduction interface t ... | 1998 | 10096825 |
| the effects of herbal oxytocics on the isolated "stripped" myometrium model. | decoctions of agapanthus africanus and clivia miniata are used as oxytocic agents in south african traditional herbal medicine. aqueous extracts of a. africanus and c. miniata leaves have been shown to possess similar uterotonic activities in the isolated whole uterus preparation. the uterus however, comprises a myometrial and an endometrial layer and the activity of both oxytocin and the prostaglandins differs in these layers. the aim of this study was to determine the uterotonic activity of th ... | 2000 | 10972206 |
| supercritical fluid extraction and analysis of compounds from clivia miniata for uterotonic activity. | in this descriptive study, the superciritical fluid extract of the roots of clivia miniata l. was tested for uterotonic activity using guinea pig uterine smooth muscle in vitro. extraction was performed with water modified supercritical carbon dioxide at 400 atm and 80 degrees c. the uterine contractions induced by this extract were compared to those induced by the aqueous extract and found to be active at lower doses. the active compounds were isolated and the structures elucidated by spectrosc ... | 2001 | 11488461 |
| discrimination between the functional and biochemical effects of two herbal oxytocics on the rat myometrium. | this study on the rat myometrium is the first report where the effects of herbal extracts used as oxytocics in traditional medicine have been systematically analysed in the same preparation at the level of functional (contractile) and biochemical (second messenger generation) responses. extracts of agapanthus africanus and clivia miniata (used in south african traditional medicine) were compared with other uterotonic agents with regard to their ability to stimulate phosphoinositide metabolism in ... | 2001 | 11518025 |
| [molecular cloning and analysis of a monocot mannose-binding agglutinin from zephyranthes grandiflora (family amaryllidaceae)]. | the monocot mannose-binding lectin can inhibit hiv from infecting the target cells. the total rna of zephyranthes grandiflora was extracted and reversely transcribed into cdna. degenerate primers were designed based on the conserved regions of other monocot mannose-binding agglutinins by homology alignment. the 694bp full-length cdna of zephyranthes grandiflora agglutinin (zga) was cloned by rt-pcr, 3' and 5' race (rapid amplification of cdna ends). the start codon and stop codon of zga were at ... | 2004 | 15553865 |
| intracuticular wax fixes and restricts strain in leaf and fruit cuticles. | this paper investigates the effects of cuticular wax on the release of strain and on the tensile properties of enzymatically isolated cuticular membranes (cms) taken from leaves of agave (agave americana), bush lily (clivia miniata), holly (ilex aquifolium), and ivy (hedera helix) and from fruit of apple (malus × domestica), pear (pyrus communis), and tomato (lycopersicon esculentum). biaxial strain release was quantified as the decrease in cm disc area following wax extraction. stiffness, maxim ... | 2013 | 23750808 |
| characterization and biosynthesis of non-degradable polymers in plant cuticles. | the structure and monomeric composition of the highly aliphatic and non-saponifiable fraction of cutans isolated from the leaf cuticles of agave americana l. and clivia miniata reg. have been elucidated. spectroscopic fourier transform infrared and 13c-nuclear magnetic resonance, calorimetric and x-ray diffraction studies, together with biopolymer analysis after exhaustive ozonolysis, showed that the cutan fraction consists of an amorphous three-dimensional network linked by ether bonds containi ... | 1999 | 10333583 |
| accumulation and transport of (2,4-dichlorophenoxy)acetic acid in plant cuticles: i. sorption in the cuticular membrane and its components. | partition coefficients (k) of (2,4-dichlorophenoxy)acetic acid (2,4-d) have been determined for the system plant cuticle/aqueous buffer. cuticles isolated enzymatically from leaves (clivia miniata, ficus elastica, citrus aurantium, hedera helix, pyrus communis cv. conférence and cv. williams, olea europaea) and fruits (lycopersicon esculentum, capsicum annuum, solanum melongena, cucumis sativus) were utilized. only the nondissociated species of 2,4-d was sorbed by cuticles and their lipophilic c ... | 1984 | 6734501 |
| fourier transform infrared-spectroscopic characterisation of isolated endodermal cell walls from plant roots: chemical nature in relation to anatomical development | the chemical nature of enzymatically isolated endodermal cell walls from cicer arietinum l., clivia miniata reg. and iris germanica l. was studied by ftir (fourier transform infrared) spectroscopy. observed frequencies were assigned to functional groups present in the cell wall and relative amounts of the biopolymers suberin and lignin, cell wall carbohydrates and proteins were determined. infrared absorption spectra indicated structural characteristics for the three different developmental stat ... | 1999 | 10550636 |
| leaf patterning of clivia miniata var. variegata is associated with differential dna methylation. | leaf patterns (yellow, green and striped) of clivia miniata var. variegata might be caused by differential dna methylation in ccgg sites in response to heterogeneous environmental pressure. clivia miniata is an important ornamental plant.clivia miniata var. variegata (cmvv) is a variegated leaf mutant of c. miniata. typical cmvv has attractive green and yellow-stripped leaves. the study has revealed that an explant of cmvv, even a full-green explant, could regenerate plants of three different ty ... | 2016 | 26466593 |
| isolation and identification of fourteen microsatellite markers in clivia miniata and clivia nobilis (amaryllidaceae). | clivia is a genus of great horticultural importance and has been widely cultivated as ornamental plants in all over the world. in order to assess the phylogenetic relationships and genetic diversity of the wild clivia species and cultivars, we isolated ac-enriched repeats using fiasco from a single clone each of c. miniata regel. and clivia nobilis lindl. of the fourteen repeats, 10 were polymorphic and 4 were monomorphic. the polymorphic marker loci were characterized using 61 clivia accessions ... | 2012 | 22949819 |
| direct and indirect organogenesis of clivia miniata and assessment of dna methylation changes in various regenerated plantlets. | clivia miniata is an important indoor ornamental plant and has been reported to have medicinal value. we developed an efficient in vitro micropropagation protocol from young leaves (indirect organogenesis), young petals (indirect organogenesis) and shoot tips (direct organogenesis) of this plant. using young leaves and shoot tips as explants, the regeneration frequencies were much higher than those in previous investigation and the regeneration was dependent upon less nutrition. we speculated th ... | 2012 | 22532007 |
| circadian rhythms in electrical circuits of clivia miniata. | the biological clock regulates a wide range of physiological processes in plants. here we show circadian variation of the clivia miniata responses to electrical stimulation. the biologically closed electrochemical circuits in the leaves of c. miniata (kaffir lily), which regulate its physiology, were analyzed in vivo using the charge stimulation method. the electrostimulation was provided with different voltages and electrical charges. resistance between ag/agcl electrodes in the leaf of c. mini ... | 2011 | 21546115 |
| changes in negative air ions concentration under different light intensities and development of a model to relate light intensity to directional change. | the purpose of this research was to determine changes in negative air ion (nai) concentrations produced by plants grown under different light intensities. nai concentrations were observed and analyzed in an outdoor green space with five plant species (aloe arborescens, clivia miniata, chlorophytum comosum, opuntia brunnescens, crassula portulacea) and a. arborescens was then grown under controlled-light conditions in an enclosed space for the subsequent light intensity experiment. | 2009 | 19356839 |
| molecular cloning of a new lectin gene from z. grandiflora. | using rna extracted from zephyrathes grandiflora young leaves and primers designed according to the conservative regions of amaryllidaceae lectins, the full-length cdna of z. grandiflora agglutinin (zga) was cloned by rapid amplification of cdna ends (race). the full-length cdna of zga was 934 bp and contained a 576 bp open reading frame (orf) encoding a 191 amino acid protein. through comparative analysis of zga gene and its deduced amino acid sequence with those of other amaryllidaceae species ... | 2003 | 14631657 |
| senescence vs. changes in nuclear dna, protein and dry mass contents in leaf mesophyll of two species differing in occurrence of endomitotic polyploidy. | dna and naphtol yellow s-staining (f-nys) protein contents were measured cytophotometrically using the feulgen method in the nuclei of the mesophyll from the basal and apical zone of young and old leaves in two perennial monocotyledonous species: rhoeo discolor and clivia miniata, differing in presence or absence of dna endoreplication. dry mass content was determined interferometrically using an uniform field with large image shearing method. it has been shown that nuclei with 2c dna and below ... | 1999 | 10091949 |
| chemical composition of hypodermal and endodermal cell walls and xylem vessels isolated from clivia miniata (identification of the biopolymers lignin and suberin). | the occurrence of the biopolymers lignin and suberin was investigated with hypodermal (hcw) and endodermal cell walls (ecw) and xylem vessels (xv) isolated from clivia miniata reg. roots. both biopolymers were detected in hcw and ecw, whereas in xv, typical aliphatic suberin monomers were missing and only representative lignin monomers such as guaiacyl (g) and syringyl (s) units could be detected. the absolute amounts of lignin were about one order of magnitude higher compared with suberin in bo ... | 1997 | 12223670 |
| molecular cloning of mannose-binding lectins from clivia miniata. | screening of a cdna library constructed from total rna isolated from young developing ovaries of clivia miniata regel with the amaryllis lectin cdna clone resulted in the isolation of four different isolectin clones which clearly differ from each other in their nucleotide sequences and hence also in their deduced amino acid sequences. apparently the lectin is translated from an mrna of ca. 800 nucleotides encoding a precursor polypeptide of 163 amino acids. northern blot analysis of total rna is ... | 1994 | 8193308 |
| local motion and conformational changes in the cuticle of clivia miniata regel : a microfluorescence and spin-label study. | the temperature dependence of the local diffusion of fluorescent molecular probes of various polarities (alkane, long-chain fatty acid, short-chain alcohol and fatty acid), all labelled with 7-nitrobenz-2-oxa-1,3-diazol-4-yl in the cuticle of clivia miniata regel was studied by the technique of fluorescence recovery after photobleaching. the technique yields the coefficient of diffusion, d, in the plane of the cuticle over distances of some 10 μm and the fraction, r, of mobile reporter molecules ... | 1990 | 24196927 |
| development of plant cuticles: fine structure and cutin composition of clivia miniata reg. leaves. | the fine structure and monomeric composition of the ester-cutin fraction (susceptible to bf3/ch3oh transesterification) of the adaxial leaf cuticle of clivia miniata reg. were studied in relation to leaf and cuticle development. clivia leaves grow at their base such that cuticle and tissues increase in age from the base to the tip. the zone of maximum growth (cell expansion) was located between 1 and 4 cm from the base. during cell expansion, the projected surface area of the upper epidermal cel ... | 1988 | 24221429 |
| in-vivo study of cutin synthesis in leaves of clivia miniata reg. | cutin synthesis of clivia miniata reg. was studied by using intact leaves. tritium-labelled hexadecanoic acid was used as precursor and was administered as droplets of micellar solutions to the upper surface of expanding leaves. radiolabel was incorporated rapidly. within 2 h, up to 10% of the label administered had been incorporated into cutin. rates of (3)h-cutin synthesis depended on the position of the site of precursor donation to the leaf. highest rates were observed between 3 to 4 cm from ... | 1983 | 24264450 |
| development of plant cuticles: occurrence and role of non-ester bonds in cutin of clivia miniata reg. leaves. | the effect of bf3-methanol treatment on the mass and fine structure of isolated clivia leaf cuticles at different stages of development has been investigated. bf3-methanol cleaves ester linkages in cutin; however, the cuticles are not completely depolymerized. with increasing age, the residue left after bf3-methanol treatment increases in mass. in very young cuticles, 10% of the total cutin resisted bf3-methanol and the fraction of nonester cutin increased up to 62% in mature leaves. transmissio ... | 1982 | 24272585 |
| plant antiviral agents. iii. isolation of alkaloids from clivia miniata regel (amaryllidaceae). | lycorine (1), one of the main alkaloids of the amaryllidaceae family, was found to be responsible for the pronounced antiviral activity of the crude extracts from the roots and leaves of clivia miniata regel. it was shown that the inhibitory activity of lycorine on the cytopathogenic effect caused by a dna and several rna viruses on vero cells was not virucidal. poliomyelitis virus inhibition occurred at lycorine concentrations as low as 1 microgram/ml, whereas concentrations exceeding 25 microg ... | 2006 | 7153775 |
| fine structure of plant cuticles in relation to water permeability: the fine structure of the cuticle of clivia miniata reg. leaves. | the fine structure of the upper cuticular membrane (cm) of clivia miniata leaves was investigated using electron microscopy. the cm is made up of a thin (130 nm) lamellated cuticle proper (cp) and a thick (up to 7 μm over periclinal walls) cuticular layer (cl) of marbled appearance. evidence is presented to show that the electron lucent lamellae of the cp do not simply represent layers of soluble cuticular lipids (scl). instead, the lamellation is probably due to layers of cutin differing in pol ... | 1981 | 24302425 |
| [radioautographic study of the incorporation of thymidineh3, uridineh3 and phenylalanineh3 into the chloroplasts of clivia miniata and bilbergia sp]. | | 1964 | 5856311 |
| [nuclear structures in the endopolyploid antipodes of clivia miniata]. | | 1957 | 13523743 |