| thin layer chromatographic determination of sterigmatocystin in cereal grains and soybeans. | a method has been developed for determining sterigmatocystin in yellow and white corn, barley, oats, rye, sorghum, brown and wild rice, and soybeans. a partition column packed with activated magnesium silicate was used for cleanup. averaged recoveries are 104% in white corn, 114% in rye, 100% in oats, 134% in brown rice, 96% in barley, 105% in sorghum and wild rice, and 92% in soybeans. the limit of sensitivity is 50 mug/kg for any of these commodities. | 1976 | 987035 |
| retrotransposon families in rice. | three families of retrotransposons of rice (tos1, tos2, and tos3) were isolated by using a method based on the sequence conservation of the primer binding site for reverse transcription. this method should be generally applicable for cloning retrotransposon of other plants. one retrotransposon, tos3-1, was studied in detail. tos3-1 is 5.2 kb long, has structures common to retrotransposons, such as long terminal repeats (ltr), a primer binding site complementary to the initiator trna, a polypurin ... | 1992 | 1376404 |
| an evaluation of the mosquitofish, gambusia affinis, and the inland silverside, menidia beryllina, as mosquito control agents in california wild rice fields. | the mosquitofish, gambusia affinis, and the inland silverside, menidia beryllina, were evaluated in experimental, one-tenth hectare wild rice plots in lake county, california, for their impact on densities of culex tarsalis, anopheles freeborni and anopheles franciscanus. gambusia affinis were tested at 0.6 and 1.7 kg/ha and the silversides at ca. 0.9 kg/ha. the silversides did not survive well in the rice field system and none of the silverside guts examined contained mosquito larvae. the mosqu ... | 1987 | 2904970 |
| molecular characterization of two endogenous double-stranded rnas in rice and their inheritance by interspecific hybrids. | we completely sequenced 13,936 nucleotides (nt) of a double-stranded rna (dsrna) of wild rice (w-dsrna). a single long open reading frame (13,719 nt) containing the conserved motifs of rna-dependent rna polymerase and rna helicase was located in the coding strand. the identity between entire nucleotide sequence of w-dsrna and that of the dsrna of temperate japonica rice (j-dsrna, 13,952 nt) was 75.5%. a site-specific discontinuity (nick) was identified at nt 1,197 from the 5' end of the coding s ... | 1999 | 10066741 |
| expansion of a (ga) dinucleotide at a microsatellite locus associated with domestication in rice. | microsatellites undergo rapid changes over short evolutionary time periods which can be phylogenetically informative in related species. here we show the repeat unit expansion of a (ga)n-type microsatellite in the process of cultivation of rice from its wild ancestors. we amplified a microsatellite locus harboring (ga)n repeats from several wild and cultivated rices. sequencing revealed an increase in repeat number from 14 in distantly related wild rice species to 24 in the widely grown present- ... | 1998 | 9919358 |
| in vitro propagation of salt-tolerant wild rice relative, porteresia coarctata tateoka. | micropropagation of porteresia coarctata tateoka, a wild relative of rice with useful genetic traits of salinity and submergence tolerance, was achieved through nodal segment culture. woody plant (wp) medium supplemented with benzyladenine (5.5 µm) and kinetin (2.3 µm) gave the greatest response to initiation and multiplication. the multiplication rate of 11 shoots/explant with an average shoot length of 3.5 cm was observed after 8 weeks of culture period. the rooting response was observed simul ... | 1998 | 9892746 |
| developmental events associated with the critical stage for sex determination in wild-rice florets. | the developmental events of florets and a critical stage for sex determination in two wild-rice populations (zizania palustris cv. franklin and zizania palustris cv. k-2pi) have been identified. formation of bisexual florets precedes the development of both male and female florets. developmental indicators, established by measuring the length of florets and panicles, indicate that the critical stage for sex determination occurs when floret and panicle lengths are 1-2 mm and 3 cm, respectively. t ... | 1999 | 10568779 |
| identification of trait-improving quantitative trait loci alleles from a wild rice relative, oryza rufipogon. | wild species are valued as a unique source of genetic variation, but they have rarely been used for the genetic improvement of quantitative traits. to identify trait-improving quantitative trait loci (qtl) alleles from exotic species, an accession of oryza rufipogon, a relative of cultivated rice, was chosen on the basis of a genetic diversity study. an interspecific bc2 testcross population (v20a/o. rufipogon//v20b///v20b////ce64) consisting of 300 families was evaluated for 12 agronomically im ... | 1998 | 9755218 |
| rire1, a retrotransposon from wild rice oryza australiensis. | rire1 is a retrotransposon present in wild rice oryza australiensis in an extraordinary number of copies, and only a portion of the ltr sequence has been determined previously. here, we isolated and sequenced dna segments of various portions of rire1, revealing that the sequences of ltr and the internal region were 1523 and 5277 bp in length, respectively. the internal region shows homology with the pol region in copia, a drosophila retrotransposon, indicating that rire1 is a copia-like retrotra ... | 1997 | 9339541 |
| copia-like retrotransposons in rice: sequence heterogeneity, species distribution and chromosomal locations. | degenerated oligonucleotide primers were used to amplify, clone, and analyze sequence heterogeneity and chromosomal distribution of 23 pcr fragments corresponding to the reverse transcriptase domain of copia-like retrotransposons in rice. of the 23 fragments 22 could be aligned by their deduced amino acid sequences and were divided into 6 groups according to the phylogenetic and southern blot analyses. amino acid sequence differences among the 22 aligned fragments ranged from 1 to 64%. southern ... | 1997 | 9154986 |
| identification and characterization of two tandem repeat sequences (trsb and trsc) and a retrotransposon (rire1) as genome-general sequences in rice. | three kinds of dna sequences (here called trsb, trsc and rire1) have been previously reported to be those repeated in tandem specifically in the wild rice species with ff, cc or ee genome, respectively. to characterize these genome type-specific sequences, we carried out pcr using a pair of primers, which hybridize to a restricted region in the repeating unit sequence and prime dna synthesis in both directions. gel electrophoresis and dna sequencing revealed that pcr using primers for trsb (or t ... | 1996 | 9080684 |
| chloroplast dna variability in wild and cultivated rice (oryza spp.) revealed by polymorphic chloroplast simple sequence repeats. | short mononucleotide repeats analogous to nuclear microsatellites or simple sequence repeats (ssrs) have been identified in chloroplast genomes. primers flanking mononucleotide repeats in the fully sequenced rice chloroplast genome have been used in conjunction with pcr to amplify genomic dna from 42 wild rice accessions. the amplification products exhibited length polymorphism, which allowed the levels of chloroplast variability detected to be quantified. seven primer pairs that amplified produ ... | 1997 | 9061917 |
| development of microsatellite markers and characterization of simple sequence length polymorphism (sslp) in rice (oryza sativa l.). | microsatellite markers containing simple sequence repeats (ssr) are a valuable tool for genetic analysis. our objective is to augment the existing rflp map of rice with simple sequence length polymorphisms (sslp). in this study, we describe 20 new microsatellite markers that have been assigned to positions along the rice chromosomes, characterized for their allelic diversity in cultivated and wild rice, and tested for amplification in distantly related species. our results indicate that the geno ... | 1996 | 8914521 |
| photosynthetic bradyrhizobia are natural endophytes of the african wild rice oryza breviligulata. | we investigated the presence of endophytic rhizobia within the roots of the wetland wild rice oryza breviligulata, which is the ancestor of the african cultivated rice oryza glaberrima. this primitive rice species grows in the same wetland sites as aeschynomene sensitiva, an aquatic stem-nodulated legume associated with photosynthetic strains of bradyrhizobium. twenty endophytic and aquatic isolates were obtained at three different sites in west africa (senegal and guinea) from nodal roots of o. ... | 2000 | 11097925 |
| complex arrangement of dispersed repeated dna sequences in oryza officinalis. | a 525-bp bglii fragment was isolated from oryza officinalis dna (accession w1278) and shown to correspond to a new dispersed repetitive dna sequence with specificity restricted to a subset of the wild rice with a c genome. the sequence of the fragment was determined but it does not correspond to any sequence already present in databases. it contains several imperfect palindromes. larger genomic clones (12-18 kbp) were isolated and all contain sequences homologous to the bglii element. analysis o ... | 1996 | 8851805 |
| studies of the safety of chinese wild rice. | chinese wild rice has been consumed for over 3000 years, but its safety as a food in china has never been established. the grain contains higher amounts of protein, ash and crude fibre than white rice. levels of non-nutritive mineral elements such as arsenic, cadmium and lead are very low. the eating patterns of 110 people ( > 60 yr) showed no ill-effects. the results of acute toxicity tests with mice fed diet containing 21.5 g/kg chinese wild rice [corrected] indicated no abnormal reaction and ... | 1996 | 8641660 |
| cadmium, copper and lead in wild rice from central canada. | samples of wild rice grains available for sale in manitoba, canada contained < 0.01-6.2 micrograms/g cadmium, < 0.01-6.7 micrograms/g lead, and 1.6-14.4 micrograms/g copper. rice originating from saskatchewan and northern manitoba contained significantly higher copper concentrations than samples from ontario and southeastern manitoba. larger and heavier grains contained higher lead concentrations per unit weight. mean content of all three metals per individual grain was exponentially correlated ... | 1993 | 8466300 |
| characterization of new variants of a satellite dna from oryza officinalis, specific for the cc genome of wild rice. | two groups of tandemly repeated dna fragments have been isolated and cloned from the oryza officinalis genome (w 1278). these fragments have been sequenced and are 374 and 367 bp long. they were compared with an element previously isolated from another o. officinalis accession. they are 76 and 92% homologous to the latter and show 78% homology between themselves. the two types of elements are interspersed within large blocks of tandemly organized units. their copy number is in the range of 200,0 ... | 1993 | 7916734 |
| analysis of random and specific sequences of nuclear and cytoplasmic dna in diploid and tetraploid american wild rice species (oryza spp.). | a sample of american wild rice and other accessions of the genus oryza were studied at polymorphic regions of nuclear, mitochondrial, and chloroplastic genomes. first, flow cytometry, genome-specific rapd markers, and chromosome counting were utilized to verify the original ploidy and classification of 230 accessions studied. based on these methods, 8% of the accessions were considered to be misclassified either taxonomically or as a result of contamination. second, a fine resolution analysis wa ... | 2001 | 11444708 |
| comparative rflp mapping of an allotetraploid wild rice species (oryza latifolia) and cultivated rice (o. sativa). | the purpose of this study was to construct a comparative rflp map of an allotetraploid wild rice species, oryza latifolia, and to study the relationship between the ccdd genome of o. latifolia and the aa genome of o. sativa. a set of rflp markers, which had been previously mapped to the aa genome of cultivated rice, were used to construct the comparative map. fifty-eight f2 progeny, which were derived from a single f1 plant, were used for segregation analysis. the comparative rflp map contains 1 ... | 1994 | 7914758 |
| comparative rflp mapping of a wild rice, oryza officinalis, and cultivated rice, o. sativa. | a comparative rflp map was constructed in a wild rice, oryza officinalis, by using 139 genomic and cdna probes that had been used previously to map rflps in o. sativa. nine of the 12 chromosomes of o. officinalis were highly homosequential to those of o. sativa. a major rearrangement of gene order was detected in chromosome 1 and small inversions were found in chromosomes 3 and 11. fourteen translocated rflp markers were found, and chromosome 11 contained a high frequency of such translocated se ... | 1994 | 7913453 |
| characterization of a dispersed repetitive dna sequence associated with the ccdd genome of wild rice. | a hindii repetitive fragment (pod3) was isolated and cloned from the genomic dna of an accession of oryza latifolia, a wild rice species that possesses a tetraploid ccdd genome. southern blot analysis using this clone as a probe demonstrated that this repetitive dna sequence had a dispersed organization in the ccdd genome and seemed to be highly specific for this genome type. this fragment is the first ccdd-specific repeated dna sequence to be described. the hybridization pattern is similar for ... | 1995 | 7672603 |
| wild rice: the indian's staple and the white man's delicacy. | wild rice (zizania aquatica) is an annual aquatic grass which grows in shallow lakes, marshes and in sluggish streams in various parts of the world. the grain of wild rice has been harvested by the indians of the united states and canada for many centuries. explorers entering the territories of the northern lake states of america a few centuries ago described wild rice as a spontaneous crop which does not require plowing or sowing providing an abundant harvest of palatable and nourishing grain. ... | 1981 | 7030624 |
| high levels of genetic differentiation of oryza officinalis wall. ex watt. from china. | in order to determine the population genetic structure of wild rice (oryza officinalis wall. ex watt.), an endangered tropical and subtropical species, allozyme diversity encoded by 24 loci was analyzed electrophoretically in 145 individuals of eight natural populations from hainan, guangxi, and yunnan provinces, china. a fairly high genetic differentiation (f(st) = 0.882 and mean i = 0.786) was found among the studied populations. our results suggest that restricted gene flow may play a signifi ... | 2001 | 11948221 |
| preliminary phytochemical investigation of wild rice ergot. | | 1968 | 5657560 |
| a wild rice seed under the upper eyelid: found after 14 months by eversion. | | 1973 | 4768213 |
| an evaluation of gambusia affinis and bacillus thuringiensis var. israelensis as mosquito control agents in california wild rice fields. | the mosquito control potential of the mosquitofish and bacillus thuringiensis var. israelensis (bti) were evaluated in experimental wild rice fields in lake county, california. fields were assigned one of six treatment: control, 1.1 kg/ha g. affinis, 3.4 kg/ha g. affinis, bti only (6 kg/ha vectobac granules), 1.1 kg/ha g. affinis plus bti and 3.4 kg/ha g. affinis plus bti. gambusia affinis, at both release rates, significantly reduced the mosquito population at densities exceeding 100 fish/minno ... | 1988 | 2906358 |
| variation in the nucleotide sequence of a prolamin gene family in wild rice. | variation in the dna sequence of the 10 kda prolamin gene family within the wild rice species oryza rufipogon was probed using the direct sequencing of pcr-amplified genes. a comparison of the nucleotide and deduced amino-acid sequences of eight asian strains of o. rufipogon and one strain of the related african species o. longistaminata is presented. | 1990 | 2103437 |
| laboratory evaluation of lagenidium giganteum (oomycetes: lagenidiales) in water from contra costa county, california, mosquito sources. | six bioassays were performed to evaluate the efficacy of the fungus, lagenidium giganteum, against mosquitoes in water collected from 75 sources. the fungus infected larvae of 4 genera and produced greater than 90% mortality in water from some of the creeks, artificial containers and the wild rice field tested during 4 of the assays. there was no larval mortality due to the fungus in water from irrigated pastures or marshes. water quality parameters associated with l. giganteum infection varied ... | 1990 | 1969930 |
| cloning and characterization of repetitive dna sequences from genomes of oryza minuta and oryza australiensis. | the value of genome-specific repetitive dna sequences for use as molecular markers in studying genome differentiation was investigated. five repetitive dna sequences from wild species of rice were cloned. four of the clones, pom1, pom4, poma536, and pompb10, were isolated from oryza minuta accession 101141 (bbcc genomes), and one clone, poa237, was isolated from oryza australiensis accession 100882 (ee genome). southern blot hybridization to different rice genomes showed strong hybridization of ... | 1991 | 1955157 |
| restriction fragment length polymorphism analysis of ccdd genome species of the genus oryza l. | restriction fragment length polymorphisms (rflps) were studied in fourteen accessions of ccdd genome allotetraploid wild rice species (oryza latifolia, o. alta and o. grandiglumis). fourteen nuclear rflp markers previously mapped in aa genome-cultivated rice were used as probes. a phylogenetic tree, constructed by parsimony analysis based on rflps, grouped the accessions according to their geographic origin from central or south america. oryza alta, o. grandiglumis and one accession of o. latifo ... | 1991 | 1677594 |
| a cryopreservation protocol for embryos of the endangered species zizania texana. | seeds of the endangered species zizania texana are recalcitrant, making it difficult to preserve the remaining genetic diversity of this species in genebanks. excised embryos can be cryopreserved using solution-based cryoprotection protocols. survival following cryoexposure increased from less than 5% to about 75% by preculturing embryos in high concentrations of sugars, bathing them in cryoprotectant solutions, and partially drying them to water contents of about 0.6 g h2o/g dry mass. | 2002 | 12447488 |
| wild rice as fermentation substrate for mycotoxin production. | many cereal grains have been studied for their suitability as substrates for the fermentative production of mycotoxins. however, except for aflatoxin, wild rice has not been investigated. hence, five mold cultures known to produce the mycotoxins ochratoxin-a, penicillic acid, patulin, vomitoxin, and zearalenone were grown on wild rice under varying conditions of moisture and temperature to determine whether this grain would serve as a suitable substrate for toxin production. under appropriate fe ... | 1978 | 623456 |
| unusual inheritance of evolutionarily-related double-stranded rnas in interspecific hybrid between rice plants oryza sativa and oryza rufipogon. | endogenous, 14 kb double-stranded rnas (dsrnas) have been found in two ecospecies of cultivated rice (temperate japonica rice and tropical japonica rice, oryza sativa l.) and in wild rice (o. rufipogon, an ancestor of o. sativa). a comparison of the nucleotide and deduced amino acid sequences of the core regions of the rna-dependent rna polymerase domains found in these three dsrnas suggested that these dsrnas probably evolved independently within each host plant from a common ancestor. these ds ... | 1999 | 10380800 |
| rapd variation within and between natural populations of the wild rice oryza rufipogon from china and brazil | genetic variation within and between eight natural populations of oryza rufipogon from china and brazil was investigated at the dna level by analysis of rapd fragments. out of 60 random primers, which were initially screened against dna from four individuals, 20 generated highly reproducible rapd fragments which were then used for further population analysis. with these primers, 95 discernible dna fragments were produced and 78 (82.1%) were polymorphic, which indicated that high levels of geneti ... | 1999 | 10383685 |
| phylogeny of rice genomes with emphasis on origins of allotetraploid species. | the rice genus, oryza, which comprises 23 species and 9 recognized genome types, represents an enormous gene pool for genetic improvement of rice cultivars. clarification of phylogenetic relationships of rice genomes is critical for effective utilization of the wild rice germ plasm. by generating and comparing two nuclear gene (adh1 and adh2) trees and a chloroplast gene (matk) tree of all rice species, phylogenetic relationships among the rice genomes were inferred. origins of the allotetraploi ... | 1999 | 10588717 |
| heavy metals in wild rice from northern wisconsin. | wild rice grain samples from various parts of the world have been found to have elevated concentrations of heavy metals, raising concern for potential effects on human health. it was hypothesized that wild rice from north-central wisconsin could potentially have elevated concentrations of some heavy metals because of possible exposure to these elements from the atmosphere or from water and sediments. in addition, no studies of heavy metals in wild rice from wisconsin had been performed, and a ba ... | 2000 | 10696727 |
| development of male flowers in zizania aquatica (north american wild-rice; gramineae). | we investigated the histology and developmental morphology of flowers of wild-rice (zizania aquatica), a member of grass subfamily oryzoideae, to determine whether male flowers in this species develop in a manner similar to those in the subfamily panicoideae, a group that includes many species with unisexual flowers. zizania has evolved unisexual flowers from hermaphrodite ancestors and is only distantly related to the panicoideae; the origins of unisexual flowers are independent in the two taxa ... | 2000 | 10817969 |
| [a study of the genetic diversity of common wild rice (o. rufipogon griff.) and cultivated rice (o. sativa l.) by rflp analysis]. | the genetic diversity of cultivated rice and common wild rice from 10 countries in asia were evaluated by rflp analysis using 44 probes of single copy. we used the parameters such as proportion of polymorphic loci (p), average number of alleles per locus (a), average number genotype per locus (ng), degree of heterozygate per locus (ho) and average gene diversity (hs) showing genetic diversity. it is indicated that the common wild rice from china has the largest genetic diversity in the 10 countr ... | 2000 | 10887694 |
| water loss and viability in zizania (poaceae) seeds during short-term desiccation. | how texas wild rice, zizania: texana, became isolated in the san marcos river of central texas, hundreds of kilometres from other wild rice populations is not known. zizania: seeds are intolerant of short-term desiccation. seeds desiccated at 14% relative humidity (rh) and 75% rh do not survive after only 5-6 d and 2-3 wk of drying. water loss is rapid and reaches a maximum at the time of seed death due to drying. and although all zizania: seeds germinate well following a long, cold dormancy per ... | 2000 | 11080122 |
| retrotransposon activation followed by rapid repression in introgressed rice plants. | plant retrotransposons are largely inactive during normal development, but may be activated by stresses. both copia-like and gypsy-like retrotransposons of rice were activated by introgression of dna from the wild species zizania latifolia griseb. the copy number increase was associated with cytosine methylation changes of the elements. activity of the elements was ephemeral, as evidenced by nearly identical genomic southern hybridization patterns among randomly chosen individuals both within an ... | 2000 | 11081978 |
| characterization of resistance to three bruchid species (callosobruchus spp., coleoptera, bruchidae) in cultivated rice bean (vigna umbellata). | resistance of wild and cultivated rice bean (vigna umbellata [thunberg] ohwi and ohashi) to three bruchid species, callosobruchus chinensis l., callosobruchus maculatus f., and callosobruchus analis f., was evaluated. all but three accessions of cultivated, and all wild rice bean accessions tested, exhibited complete resistance to all three bruchid species. rice bean seeds with seed coat removed also showed complete resistance to the three bruchid species. results indicate that physical attribut ... | 2003 | 12650364 |
| genetic erosion in northern marginal population of the common wild rice oryza rufipogon griff. and its conservation, revealed by the change of population genetic structure. | in order to monitor genetic erosion within the northern marginal population of common wild rice oryza rufipogon griff. from dongxiang, jiangxi province, china, allozyme diversity encoded by 22 loci was analyzed electrophoretically from all the existing subpopulations in 1980, 1985 and 1994. the sample collected from the nine large subpopulations in 1980 showed the highest levels of genetic diversity (a = 1.27, p = 18.20%, ho = 0.042 and he = 0.049) and a slight deviation from hardy-weinberg expe ... | 2000 | 11206853 |
| mapping qtls influencing rice floral morphology using recombinant inbred lines derived from a cross between oryza sativa l. and oryza rufipogon griff. | to understand the genetic basis of floral traits associated with the mating system in rice, we analyzed pistil, stamen and glume traits using a recombinant inbred line population, derived from a cross between an asian cultivated rice ( oryza sativa l.), pei-kuh, and a wild rice ( oryza rufipogon griff.), w1944. quantitative trait loci (qtls) affecting floral morphology were detected by composite interval mapping using a linkage map constructed using 147 markers, mostly rflps. a total of 7, 4, 14 ... | 2003 | 12845437 |
| preferential occurrence of diazotrophic endophytes, azoarcus spp., in wild rice species and land races of oryza sativa in comparison with modern races. | several diazotrophic species of azoarcus spp. occur as endophytes in the pioneer plant kallar grass. the purpose of this study was to screen asian wild rice and cultivated oryza sativa varieties for natural association with these endophytes. populations of culturable diazotrophs in surface-sterilized roots were characterized by 16s rdna sequence analysis, and azoarcus species were identified by genomic fingerprints. a. indigens and azoarcus sp. group c were detected only rarely, whereas azoarcus ... | 2000 | 11220300 |
| crop-specific endophytic colonization by a novel, salt-tolerant, n2-fixing and phosphate-solubilizing gluconacetobacter sp. from wild rice. | a novel salt-tolerant, n2-fixing and phosphate-solubilizing, gluconacetobacter sp. (pa12) tagged with gusa gene, colonized porteresia coarctata (wild rice) and pokkali (salt-tolerant variety) more intensively when compared to ponni (salt-sensitive variety). this was confirmed using a colony-counting method. | 2003 | 12882278 |
| inositol hexaphosphate, a natural substance found in whole kernel corn and brown rice, activates natural killer cell function - inhibits cancer. | dr. abulkalam shamsuddin has written a book discussing how diets high in bran or inositol hexaphosphate (ip6) inhibit cancer development in laboratory animals. studies show ip6 can help fight bacterial and fungal infections. the substance is found in the germ or bran portions of whole grains; the highest concentration is found in whole-kernel corn. ip6 is available in health food stores for $25 for a 120-capsule bottle, which would last 6 days at the recommended dosage. ip6 can be added to one?s ... | 1998 | 11366552 |
| development and mapping of oryza glumaepatula-derived microsatellite markers in the interspecific cross oryza glumaepatula x o. sativa. | wild germplasm of domesticated crops is a source of genetic variation little utilized in breeding programs. interspecific crosses can potentially uncover novel gene combinations that can be important for quantitative trait analysis. the combined use of wide crosses and genetic maps of chromosomal regions associated with quantitative traits can be used to broaden the genetic basis of rice breeding programs. oryza glumaepatula is a diploid (aa genome) wild rice species native from south and centra ... | 2001 | 11525066 |
| [qtl analysis of anther length and ratio of stigma exsertion, two key traits of classification for cultivated rice (oryza sativa l.) and common wild rice (o. rufipogon griff.)]. | a genetic map including 120 rflp markers covering 1418.2 cm has been constructed using a bc1 population derived from a cross between common wild rice (oryza rufipogon griff.) from dongxiang, jiangxi province, china, and an elite indica variety guichao 2. the linkage orders and genetic distances of markers in this map are consistent with the map constructed by rice genome program of japan except the markers of short arm of chromosome 1. the qtls analysis of anther length and ratio of stigma exser ... | 2001 | 11554349 |
| endophytic colonization and in planta nitrogen fixation by a herbaspirillum sp. isolated from wild rice species. | nitrogen-fixing bacteria were isolated from the stems of wild and cultivated rice on a modified rennie medium. based on 16s ribosomal dna (rdna) sequences, the diazotrophic isolates were phylogenetically close to four genera: herbaspirillum, ideonella, enterobacter, and azospirillum. phenotypic properties and signature sequences of 16s rdna indicated that three isolates (b65, b501, and b512) belong to the herbaspirillum genus. to examine whether herbaspirillum sp. strain b501 isolated from wild ... | 2001 | 11679357 |
| a novel gene organization: intronic snorna gene clusters from oryza sativa. | based on the analysis of structural features and conserved elements, 27 novel snorna genes have been identified from rice. all of them belong to the c/d box-containing snorna family except for one that belongs to the h/aca box type. the newly found genes fall into six clusters that comprise at least three snorna genes, and in one case as many as nine genes. interestingly, four of the six clusters are located within the largest intron of a protein coding gene. the majority of intronic snorna gene ... | 2002 | 12136108 |
| recovery of embryos of zizania palustris following exposure to liquid nitrogen. | factors affecting survival of embryos of zizania palustris after exposure to liquid nitrogen were investigated. moisture content was the most critical factor. embryos desiccated to a moisture content between 0.36 and 0.56 g water per g dry weight had highest survival after liquid nitrogen exposure (56 +/- 4% and 52 +/- 9% respectively). the recovery of embryos in the light following liquid nitrogen exposure also influenced survival. survival increased from 35 +/- 4% to 56 +/- 4% when embryos con ... | 2000 | 12148031 |
| molecular mapping and genetic analysis of a rice brown planthopper (nilaparvata lugens stål) resistance gene. | the brown planthopper (bph), nilaparvata lugens stål, is a serious insect pest of rice (oryza saliva l.). we have determined the chromosomal location of a bph resistance gene in rice using ssr and rflp techniques. a rice line 'b14', derived from the wild rice oryza latifolia, showed high resistance to bph. for tagging the resistance gene in 'b14x', an f2 population and a recombinant inbred (ri) population from a cross between taichung native 1 and 'b14' were developed and evaluated for bph resis ... | 2002 | 12184487 |
| development of desiccation tolerance during embryogenesis in rice (oryza sativa) and wild rice (zizania palustris) (dehydrin expression, abscisic acid content, and sucrose accumulation). | the ability of seeds to withstand desiccation develops during embryogenesis and differs considerably among species. paddy rice (oryza sativa l.) grains readily survive dehydration to as low as 2% water content, whereas north american wild rice (zizania palustris var interior [fasset] dore) grains are not tolerant of water contents below 6% and are sensitive to drying and imbibition conditions. during embryogenesis, dehydrin proteins, abscisic acid (aba), and saccharides are synthesized, and all ... | 1994 | 12232094 |
| a transcriptionally active maize mudr-like transposable element in rice and its relatives. | two mu-like transposable elements were cloned from a rice genomic library using a partial cdna clone that exhibits high homology to the mudra gene of the maize element mudr. database searches led to the identification of six other sequences that carried highly homologous terminal inverted repeats (tirs). all the rice elements possessed approximately 200-bp tirs, and four were flanked by 9-bp target-site duplications (tsds). the longer of the two cloned elements, osmu4-2, could potentially encode ... | 2002 | 12436254 |
| evolutionary relationships among rice species with aa genome based on sine insertion analysis. | previous studies based on morphological and molecular markers indicated that there are two cultivated and five wild rice species within the oryza genus with the aa genome. in the cultivated rice species, oryza sativa, a retroposon named p-sine1 has been identified. some of the p-sine1 members characterized previously showed interspecific insertion polymorphisms in the species with the aa genome. in this study, we identified new p-sine1 members showing interspecific insertion polymorphisms from r ... | 2002 | 12441643 |
| search for and analysis of single nucleotide polymorphisms (snps) in rice (oryza sativa, oryza rufipogon) and establishment of snp markers. | we searched for snps in 417 regions distributed throughout the genome of three oryza sativa ssp. japonica cultivars, two indica cultivars, and a wild rice (o. rufipogon). we found 2800 snps in approximately 250,000 aligned bases for an average of one snp every 89 bp, or one snp every 232 bp between two randomly selected strains. graphic representation of the frequency of snps along each chromosome showed uneven distribution of polymorphism-rich and -poor regions, but little obvious association w ... | 2002 | 12465716 |
| [study on nutrition composition and protein quality of a chinese wild rice]. | in order to exploit and develop the resource of a kind of chinese wild rice[zinania lalifolia (griseb) turcz], the nutrition values of the chinese wild rice from luoma, baoying, hongze, weishan and tai lake were analyzed. the nutrients of chinese wild rice per 100 g samples were protein 13.2 g, vitamin b1 0.59 mg, vitamin b2 0.07 mg, vitamin e 0.29 mg, zinc 1.60 mg, iron 2.69 mg, and they were higher than those of white rice. the amino acid score of chinese wild rice was 84, the first limiting a ... | 2000 | 12520960 |
| genetic differentiation for nuclear, mitochondrial and chloroplast genomes in common wild rice ( oryza rufipogon griff.) and cultivated rice ( oryza sativa l.). | the genetic differentiation of nuclear, mitochondrial (mt) and chloroplast (cp) genomes was investigated by southern and pcr analysis using 75 varieties of cultivated rice ( oryza sativa l.) and 118 strains of common wild rice (cwr, oryza rufipogon griff.) from ten countries of asia. the distinguishing differences between the indica and japonica cultivars were detected both in the nuclear genome and the cytoplasmic genome, confirming that the indica-japonica differentiation is of major importanc ... | 2002 | 12582589 |
| a genome-specific repetitive dna sequence from oryza eichingeri: characterization, localization, and introgression to o. sativa. | in the course of transferring the brown planthopper resistance from a diploid, cc-genome wild rice species, oryza eichingeri (irgc acc. 105159 and 105163), to the cultivated rice variety 02428, we have isolated many alien addition and introgression lines. the o. eichingeri chromatin in some of these lines has previously been identified using genomic in situ hybridization and molecular-marker analysis. here we cloned a tandemly repetitive dna sequence from o. eichingeri irgc acc105163, and detect ... | 2002 | 12582684 |
| genetic differentiation of wild relatives of rice as assessed by rflp analysis. | to study genetic diversity and relationships of wild relatives of rice, 58 accessions of oryza rufipogon, oryza nivara, oryza sativa f. spontanea and the cultivated oryza sativa, representing a wide range of their distribution, were analyzed using the restriction fragment length polymorphism (rflp) technique. all 30-used rflp probes detected polymorphisms among the oryza accessions, with an average of 3.8 polymorphic fragments per probe. considerable genetic diversity was scored among the oryza ... | 2002 | 12582876 |
| assessment of population genetic structure in common wild rice oryza rufipogon griff. using microsatellite and allozyme markers. | the genetic structure of five natural populations of common wild rice oryza rufipogon griff. from china, was investigated with 21 microsatellite loci and compared to estimates of genetic diversity and genetic differentiation detected by 22 allozyme loci. microsatellite loci, as expected, have much higher levels of genetic diversity (mean values of a = 3.1, p = 73.3%, ho = 0.358 and he = 0.345) than allozyme loci (mean values of a = 1.2, p = 12.7%, ho = 0.020 and he = 0.030). genetic differentiat ... | 2002 | 12582886 |
| anaerobic nitrogen-fixing consortia consisting of clostridia isolated from gramineous plants. | we report here the existence of anaerobic nitrogen-fixing consortia (anficos) consisting of n(2)-fixing clostridia and diverse nondiazotrophic bacteria in nonleguminous plants; we found these anficos while attempting to overcome a problem with culturing nitrogen-fixing microbes from various gramineous plants. a major feature of anficos is that n(2) fixation by the anaerobic clostridia is supported by the elimination of oxygen by the accompanying bacteria in the culture. in a few anficos, nondiaz ... | 2004 | 15128572 |
| genetic dissection of seed shattering, agronomic, and color traits in american wildrice ( zizania palustris var. interior l.) with a comparative map. | a comparative map of american wildrice ( zizania palustris var. interior l.) was used to identify loci controlling seed shattering, plant height, maturity, tiller number, plant habit, panicle length seed length, and color traits. two to six significant quantitative-trait-loci (qtls, p < 0.05) were detected for each trait evaluated, representing the first trait-mapping in wildrice. the chosen population was designed to emphasize the mapping of loci controlling the shattering trait, which is the m ... | 2002 | 12582936 |
| ultrastructural morphologic description of the wild rice species oryza latifolia (poaceae) in costa rica. | the wild rice species oryza latifolia is endemic to tropical america, allotetraploid and has a ccdd genome type. it belongs to the officinalis group of the genus oryza. this species is widely distributed throughout the lowlands of costa rica and it is found on different life zones, having great morphologic diversity. the purpose of this research is to perform a morphologic description of o. latifolia samples of three costa rican localities (carara, liberia and cañas) and to see if the phenotypic ... | 2003 | 15162727 |
| sinapate dehydrodimers and sinapate-ferulate heterodimers in cereal dietary fiber. | two 8-8-coupled sinapic acid dehydrodimers and at least three sinapate-ferulate heterodimers have been identified as saponification products from different insoluble and soluble cereal grain dietary fibers. the two 8-8-disinapates were authenticated by comparison of their gc retention times and mass spectra with authentic dehydrodimers synthesized from methyl or ethyl sinapate using two different single-electron metal oxidant systems. the highest amounts (481 microg/g) were found in wild rice in ... | 2003 | 12590493 |
| [study on nitrogen removal treating agriculture wastewater in subsurface constructed wetland]. | nitrogen removal in subsurface constructed wetland treating agriculture non-pointed wastewater was studied in pilot-scale. the experimental results showed that the removal rates of tn increases with hrt increasing. the removal rates of tn achieved 60% in macrophyte bed system when the nominal hydraulic retention time was 5 days. removal of tn followed first-order plug flow kinetics. rate constant of non-plant bed, phragmitas communis bed and zizania caduciflora bed was 0.14, 0.26 and 0.20 d-1 re ... | 2003 | 12708300 |
| microsatellite analysis of genetic diversity and population genetic structure of a wild rice (oryza rufipogon griff.) in china. | genetic diversity and population genetic structure of natural oryza rufipogon populations in china were studied based on ten microsatellite loci. for a total of 237 individuals of 12 populations collected from four regions, a moderate to high level of genetic diversity was observed at population levels with the number of alleles per locus ( a) ranging from 2 to 18 (average 10.6), and polymorphic loci ( p) from 40.0% to 100% (average 83.3%). the observed heterozygosity ( h(o)) varied from 0.163 t ... | 2003 | 12845446 |
| comparative genetics at the gene and chromosome levels between rice ( oryza sativa) and wildrice ( zizania palustris). | using comparative genetics, genes, repetitive dna sequences and chromosomes were studied in the oryzeae in order to more fully exploit the rice genome sequence data. of particular focus was zizania palustris l., n = 15, commonly known as american wildrice. previous work has shown that rice chromosomes 1, 4 and 9 are duplicated in wildrice. the adh1 and adh2 genes were sequenced and, based on phylogenetic analyses, found to be duplicated in wildrice. the majority of the sequence diversity in the ... | 2003 | 12904864 |
| genetic diversity in the northernmost oryza rufipogon populations estimated by ssr markers. | to estimate genetic diversity of the residual northern populations of oryza rufipogon, a total of 232 individuals from six populations were analyzed using microsatellites (ssrs). the o. rufipogon populations with different status included three from dongxiang (jiangxi province) and three from chaling (hunan province) in china. the 23 rice ssr primer pairs selected from the ricegenes database detected a total of 115 alleles, indicating that all the ssr loci were polymorphic in this study. the tot ... | 2003 | 12920513 |
| [anophelogenic significance of wild rice in water sheds]. | | 1957 | 13482792 |
| polymorphism at the esterase isozyme locus est10 associated with phylogenetic differentiation in rice. | a new esterase isozyme locus, est10, with 6 alleles including the null form, has been found in rice by using polyacrylamide gel electrophoresis. thirty f(2) populations of all possible combinations between 5 different band morphs were studied. the segregation pattern indicated that bands 1, 2, 3, 4, and the null form (0) were allelic with each other. the alleles of est10 were distributed at different frequencies among different varietal groups of rice and also between cultivated rice and its wil ... | 2003 | 14532707 |
| nucleotide polymorphism in the adh1 locus region of the wild rice oryza rufipogon. | nucleotide variation in the alcohol dehydrogenase (adh1) locus region of the wild rice oryza rufipogon and its related species was analysed to clarify the maintenance mechanism of dna variation in oryza species. the estimated nucleotide diversity in the adh1 locus region of o. rufipogon was 0.002, which was one of the lowest values detected in nuclear loci of plant species investigated so far. tests of neutrality detected significantly negative deviation from the neutral mutation model for the c ... | 2004 | 15480535 |
| [phosphorus removal of agriculture wastewater through subsurface constructed wetland]. | phosphorus removal in subsurface constructed wetland treating agriculture non-pointed wastewater was studied in pilot-scale. the experimental results showed that the removal rates of tp increased with hrt increasing when the nominal hydraulic retention time was less than 5 days. the ph decreased distinctly when the retention time was too longer in the batch and sequence style, it may bring on a lower removel rate of tp. the amount of tp that can be removed by harvesting were about 15.8% and 9.5% ... | 2003 | 14551967 |
| use of asymmetric somatic hybridization for transfer of the bacterial blight resistance trait from oryza meyeriana l. to o. sativa l. ssp. japonica. | bacterial blight is one of the major diseases affecting rice productivity. to improve the resistance of cultivated rice to bacterial blight, we introduced a bacterial blight resistance trait from oryza meyeriana, a wild rice species, into an elite japonica rice cultivar (dalixiang) using asymmetric somatic hybridization. one hundred and thirty-two independent lines were regenerated. the hybrid plants possessed several morphological features of the donor species, o. meyeriana. random amplified po ... | 2004 | 14595515 |
| gene flow from cultivated rice (oryza sativa) to its weedy and wild relatives. | transgene escape through gene flow from genetically modified (gm) crops to their wild relative species may potentially cause environmental biosafety problems. the aim of this study was to assess the extent of gene flow between cultivated rice and two of its close relatives under field conditions. | 2004 | 14602665 |
| construction of a genomic library of wild rice and agrobacterium-mediated transformation of large insert dna linked to bph resistance locus. | here we report the first genomic library of wild rice constructed on a plant-transformation-competent binary vector (bibac2) and transformation of the large insert dna into rice via agrobacterium. we selected oryza officinalis for genomic library construction. the library consists of 55,296 clones and stored in one hundred forty-four 384-well plates. random sampling of 140 clones indicated an average insert size of 71 kb at a range of 15-235 kb and 4.8% empty vectors. four wheat chloroplast prob ... | 2003 | 14636998 |
| identification of fungal ( magnaporthe grisea) stress-induced genes in wild rice ( oryza minuta). | to identify fungal stress-related genes in wild rice, oryza minuta, we constructed a subtracted library using suppression subtractive hybridization in combination with mirror orientation selection. dna chips containing 960 randomly selected cdna clones were applied by reverse northern analysis to eliminate false positive clones from the library and to prescreen differentially expressed genes. in total, 377 cdna clones were selected on the basis of their signal intensities and expression ratios. ... | 2004 | 14648106 |
| two whitebacked planthopper resistance genes in rice share the same loci with those for brown planthopper resistance. | the whitebacked planthopper (wbph), sogatella furcifera, and brown planthopper (bph) nilaparvata lugens stål are important sucking insects of rice (oryza sativa l.) crops throughout the world. rice 'b5', which has derived its resistance genes from the wild rice o. officinalis wall ex watt, is a line that is highly resistant to both wbph and bph. previously, two resistance genes against bph, qbp1, and qbp2 in 'b5' had been mapped onto chromosome 3 and chromosome 4, respectively. in this study, we ... | 2004 | 14666132 |
| fitness estimation through performance comparison of f1 hybrids with their parental species oryza rufipogon and o. sativa. | introgression of crop genes into populations of wild relatives has important implications for germplasm conservation as well as for the persistence of novel transgenes in wild populations. studies of hybrid fitness can be used to evaluate the potential for introgression to occur following episodes of interspecific hybridization. | 2004 | 14724120 |
| chiral response of oryzeae and paniceae plants in alpha-methylbenzyl-3-p-tolyl urea agar medium. | the results presented here support the hypothesis that plants of the tribe oryzeae respond enantioselectively and homogeneously to optically active 1-alpha-methylbenzyl-3-p-tolylurea (mbtu) in root growth inhibition, in contrast to echinochloa species. the oryzeae plants tested in this study belong to different genera (oryza, leersia, chikusichloa and zizania), to different species (o sativa, o glaberrima, o alta, o coarctata, o latifolia, o minuta, o rufipogon), to various ecospecies of oryza ( ... | 2004 | 14727742 |
| polyphyletic origin of cultivated rice: based on the interspersion pattern of sines. | the wild rice species oryza rufipogon with wide intraspecific variation is thought to be the progenitor of the cultivated rice species oryza sativa with two ecotypes, japonica and indica. to determine the origin of cultivated rice, subfamily members of the rice retroposon p-sine1, which show insertion polymorphism in the o. sativa -o. rufipogon population, were identified and used to "bar code" each of 101 cultivated and wild rice strains based on the presence or absence of the p-sine1 members a ... | 2003 | 12519908 |
| structures and physicochemical properties of six wild rice starches. | starches from six wild rice cultivars were studied for their chemical structures and physicochemical properties and compared with a long-grain rice starch. the six wild rice starches were similar in morphological appearance, x-ray diffraction patterns, swelling power, and water solubility index but different in amylose content, beta-amylolysis limit, branch chain length distribution, thermal properties, and pasting properties. the structure of the wild rice amylopectins was close to that of waxy ... | 2002 | 11958643 |
| rapid and reliable identification of rice genomes by rflp analysis of pcr-amplified adh genes. | the rice genus (oryza l.) consists of 24 species with 10 recognized genome types. with the realization of many useful genes in species of wild rice, continuous efforts have been made to understand their genomic composition and relationships. however, the identification of rice genomes has often been difficult owing to complex morphological variation and formation of allotetraploids. here we propose a rapid and reliable method for identifying rice genomes based on the restriction sites of pcr-amp ... | 2001 | 11768218 |
| clonality in wild rice (oryza rufipogon, poaceae) and its implications for conservation management. | correlations were examined between habitat characters and clonal structures determined by the rapd (random amplified polymorphic dna) assay in five populations of oryza rufipogon in china. nine of 175 decameric primers were used in the study because they reproducibly amplified polymorphisms. the extent of clonality together with the clonal and sexual reproductive strategies varied greatly among the five populations and correlated with the habitats where they occur. the populations under serious ... | 2001 | 11410470 |
| a novel salt-tolerant l-myo-inositol-1-phosphate synthase from porteresia coarctata (roxb.) tateoka, a halophytic wild rice: molecular cloning, bacterial overexpression, characterization, and functional introgression into tobacco-conferring salt tolerance phenotype. | l-myo-inositol-1-phosphate synthase (ec 5.5.1.4, mips), an evolutionarily conserved enzyme protein, catalyzes the synthesis of inositol, which is implicated in a number of metabolic reactions in the biological kingdom. here we report on the isolation of the gene (pino1) for a novel salt-tolerant mips from the wild halophytic rice, porteresia coarctata (roxb.) tateoka. identity of the pino1 gene was confirmed by functional complementation in a yeast inositol auxotrophic strain. comparison of the ... | 2004 | 15016817 |
| population structure and conservation genetics of wild rice oryza rufipogon (poaceae): a region-wide perspective from microsatellite variation. | oryza rufipogon griff. is the most agriculturally important but seriously endangered wild rice species. to better estimate how genetic structure can be used to obtained a conservation perspective of the species, genetic variability at six polymorphic microsatellite dna loci was examined. high levels of genetic variability were detected at six loci in 1245 individuals of 47 natural populations covering most of the species' range in china (overall rs = 3.0740, ho = 0.2290, hs = 0.6700). partitioni ... | 2004 | 15078440 |
| some effects of acidic growing conditions on three emergent macrophytes: zizania aquatica, leersia oryzoides and peltandra virginica. | transplants of zizania aquatica l., leersia oryzoides (l.) shwartz, and peltandra virginica (l.) schott and endl. were grown for ten weeks in simulated marsh conditions under acidic (ph 3.0, ph 4.0) treatment regimes and compared with controls (ph 5.6). mortality was low in all treatments and nearly all plants of z. aquatica and l. oryzoides flowered. under acidic conditions, transplant height and root biomass of z. aquatica were significantly reduced, with greater culm numbers and total lengths ... | 1992 | 15091985 |
| high-resolution genetic mapping of xa27(t), a new bacterial blight resistance gene in rice, oryza sativa l. | bacterial blight of rice, caused by xanthomonas oryzae pv. oryzae ( xoo) (ishyama) dye, is one of the serious diseases prevalent throughout asia. in a previous study, a resistance ( r) locus was transferred from the tetraploid wild rice oryza minuta to the cultivated rice species, oryza sativa l. here, we report the fine genetic mapping of the r locus, tentatively designated as xa27(t). we performed disease evaluation with an xa27(t) near-isogenic line, irbb27, testing 35 xoo strains collected f ... | 2004 | 15118822 |
| is it possible to detect echinochloa spp. tolerance to accase-inhibiting herbicides using a simple quick tolerance test? | the objective of this work was to evaluate the reliability of one quick tolerance test that would enable us, in only six days, to quantify tolerance levels of one biotype to one or more herbicides. for this purpose, we evaluated tolerance levels to cyhalofop-butyl of five echinochloa spp. biotypes: muricata, crus-galli, crus-pavonis, oryzicola and utilis, with unknown records of herbicide treatment. moreover, two biotypes of echinochloa phyllopogon were tested, one of them resistant (r) to fenox ... | 2003 | 15149127 |
| identification of genes induced in wound-treated wild rice (oryza minuta). | a subtracted library was constructed from wound-treated wild rice (oryza minuta) by suppression subtractive hybridization (ssh) in combination with mirror orientation selection (mos). to distinguish between differentially expressed transcripts and false positive clones, dna chips containing 960 random clones were applied as a form of reverse northern screening. based on the signal intensities and expression ratios obtained from experiments performed in triplicate, 371 clones were selected. ests ... | 2004 | 15179035 |
| [purification capacity of ditch wetland to agricultural non-point pollutants]. | ditch wetlands have the capacity to remove and purify non-point pollutants from agricultural drainage by sediment retention, plant absorption and microorganism decomposition. phragmites communis and zizania latifolia are two main kinds of plants growing naturally in ditch wetlands in the lower reaches of the yangtze river. they can absorb n and p efficiently, which is the main mechanism of non-point source pollutants purification by wetlands. the harvest of phragmites communis and zizania latifo ... | 2004 | 15202249 |
| swaminathania salitolerans gen. nov., sp. nov., a salt-tolerant, nitrogen-fixing and phosphate-solubilizing bacterium from wild rice (porteresia coarctata tateoka). | a novel species, swaminathania salitolerans gen. nov., sp. nov., was isolated from the rhizosphere, roots and stems of salt-tolerant, mangrove-associated wild rice (porteresia coarctata tateoka) using nitrogen-free, semi-solid lgi medium at ph 5.5. strains were gram-negative, rod-shaped and motile with peritrichous flagella. the strains grew well in the presence of 0.35% acetic acid, 3% nacl and 1% kno3, and produced acid from l-arabinose, d-glucose, glycerol, ethanol, d-mannose, d-galactose and ... | 2004 | 15280289 |
| extensive alterations in dna methylation and transcription in rice caused by introgression from zizania latifolia. | it has been demonstrated that insertion of foreign dna into mammalian genome can profoundly alter the patterns of dna methylation and transcription of the host genome. introgression of alien dna into plant genomes through sexual crossing and genetic engineering are commonly used in breeding, but it is not known if plant genomes have similar responses to alien dna introgression as those of animals. two stable rice lines with introgression from wild rice, zizania latifolia, were analyzed for patte ... | 2004 | 15316290 |
| production of bacterial blight resistant lines from somatic hybridization between oryza sativa l. and oryza meyeriana l. | novel bacterial blight (bb) resistance gene(s) for rice was (were) introduced into a cultivated japonica rice variety oryza sativa (cv. 8411), via somatic hybridization using the wild rice oryza meyeriana as the donor of the resistance gene(s). twenty-nine progenies of somatically hybridized plants were obtained. seven somatically hybridized plants and their parents were used for aflp (amplified fragment length polymorphism) analysis using 8 primer pairs. results confirmed that these plants were ... | 2004 | 15362190 |
| variation in the loss of seed dormancy during after-ripening of wild and cultivated rice species. | the aim of this paper was to verify the variation in the loss of seed dormancy during after-ripening and the interspecific and interpopulation variability in the degree of dormancy of seven wild and two cultivated rice species comprising 21 populations and two cultivars. | 2004 | 15471820 |
| [mapping of a new resistance gene to bacterial blight in rice line introgressed from oryza officinalis]. | rice line 'b5', which was derived from the wild rice oryza officinalis wall ex watt through introgression, has been proved to be high resistant to brown planthopper, whitebacked planthopper and bacterial blight (xanthomonas oryzae pv. oryzae). in this study, the resistance to bacterial blight of 187 recombinant inbred lines (rils) from a cross between ' b5' and 'minghui63' were evaluated and rflp markers linked to the resistance gene were identified by bulked segregant analysis. analysis of the ... | 2004 | 15473325 |
| [genetic diversity of common wild rice (oryza rufipogon griff) by using ssr markers and phenotypic traits in guangxi province]. | in this paper, allelic variation and genetic diversity among geographies and growth habit types were studied by using 223 accessions of common wild rice primary core collection in guangxi province, with 34 ssr primers locating on 12 chromosomes of rice and 19 phenotypic traits. in the results, 24.91 alleles were detected per locus on average with a range from 7 to 48. compared to the cultivated rice, the wild rice showed more allelic variations. the ratio of heterozygote of ssr locus was 32.01% ... | 2004 | 15493143 |
| high-resolution genetic mapping at the bph15 locus for brown planthopper resistance in rice (oryza sativa l.). | resistance to the brown planthopper (bph), nilaparvata lugens stal, a devastating sucking insect pest of rice, is an important breeding objective in rice improvement programs. bph15, one of the 17 major bph resistance genes so far identified in both cultivated and wild rice, has been identified in an introgression line, b5, and mapped on chromosome 4 flanked by restriction fragment length polymorphism markers c820 and s11182. in order to pave the way for positional cloning of this gene, we have ... | 2004 | 15549231 |
| putative replication intermediates in endornavirus, a novel genus of plant dsrna viruses. | oryza sativa endornavirus (osv) belongs to a new genus (endornavirus) and family (endoviridae) with members containing large double-stranded rna (dsrna) replicons with plasmid-like properties. analysis of products obtained from in vitro reaction of the osv rna-dependent rna polymerase revealed a rapid increase of a population of the non-coding strand rna molecules with a head-to-tail composition. northern hybridization of total rna from osv-carrier cells with riboprobes specific for the coding s ... | 2004 | 15550778 |