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properties and biodegradation of a bioemulsifier from corynebacterium hydrocarboclastus.an extracellular polymer was produced by continuous fermentation of corynebacterium hydrocarboclastus on kerosene in a 24 liter reactor. this polymer was composed of protein, lipid, and carbohydrates. the polymer possessed surface active properties, and had two critical micelle concentrations. its effectiveness was quite comparable to the effectiveness of synthetic surface active agents such as tween 80 and span 20; however, its efficiency was much lower. the polymer also had emulsifying propert ...197719111
studies on the oxidation of octane by acinetobacter calcoaceticus and flavobacterium devorans strains. 197994750
[products of bacterial destruction of sodium dodecyl sulphate].the products of na dodecyl sulphate destruction by the three bacterial cultures--pseudomonas aeruginosa, flavobacterium devorans, achromobacter guttatus--were examined. the cultures were shown to decompose na dodecyl sulphate in a similar way. the primary mechanism of destruction was found to be hydrolysis of the sulpho-ester bond in the molecule, leading to the separation of sulphate-ion and formation of dodecanol. products of bacterial destruction of alkyl sulphates did not show foam forming c ...1979117448
pseudomonas paucimobilis from a leg ulcer on a japanese seaman.pseudomonas paucimobilis was isolated in pure culture from an ulcer on the leg of a japanese seaman while in an australian port. a description of the isolate is given. this may be the first report of a human infection in which this recently characterized species is implicated as a pathogen.1979479356
septicemia caused by pseudomonas paucimobilis.a case of septicemia caused by pseudomonas paucimobilis is reported. this represents the first definitive case of disease produced by this yellow-pigmented, nonfermentative bacillus.1979507000
cellular fatty acid composition of pseudomonas paucimobilis and groups iik-2, ve-1, and ve-2.the cellular fatty acid composition of pseudomonas paucimobilis (iik-1) and group iik-2, ve-1, and ve-2 was determined by gas-liquid chromatography. the unnamed groups were readily distinguished from p. paucimobilis by cellular fatty acids. the data strongly suggest that these bacteria may be additional species of pseudomonas and flavobacterium.1979511989
meningitis caused by pseudomonas paucimobilis.this appears to be the first report of meningitis due to pseudomonas paucimobilis and the first report of a clinically significant isolate of this species in the uk. characteristics by which the species may be recognised are given, and attention is drawn to the possible confusion of ps. paucimobilis with other yellow-pigmented pseudomonads and flavobacterium species.1979512057
isolation of a novel sphingoglycolipid containing glucuronic acid and 2-hydroxy fatty acid from flavobacterium devorans atcc 10829.a new acidic sphingoglycolipid has been isolated from a gram-negative, glucose-non-fermentative (obligatory aerobic) bacterium, flavobacterium devorans atcc 10829, by thin-layer chromatography on silica gel after mild alkaline hydrolysis of the cellular lipids. chemical degradation studies, thin-layer chromatographic behavior, ir and mass-spectrometric analysis of the original and reduced glycolipid with lia1h4 revealed that the lipid contained glucuronic acid, long-chain bases, and fatty acids ...1978659393
[a case of meningitis bacteremia due to flavobacterium devorans]. 1977882026
bacteria within ovules and seeds.surface-sterilized ovules and seeds of 27 species of plants were cultured in the water of syneresis of a nutrient medium low in agar content. bacteria were obtained from 30% of the ovules, 15% of the seeds of herbaceous plants, 16% of the seeds of woody plants, 5.4% of the overwintered noncereal seeds, and 13.5% of overwintered cereal seeds. in no instance did every ovule or seed of a plant species contain bacteria. no bacteria were obtained from the hard, waxy seeds of mimosa or yellowwood. the ...1976984839
degradation of phenanthrene, fluorene and fluoranthene by pure bacterial cultures.bacterial mixed cultures able to degrade the polycyclic aromatic hydrocarbons (pah) phenanthrene, fluorene and fluoranthene, were obtained from soil using conventional enrichment techniques. from these mixed cultures three pure strains were isolated: pseudomonas paucimobilis degrading phenanthrene; p. vesicularis degrading fluorene and alcaligenes denitrificans degrading fluoranthene. the maximum rates of pah degradation ranged from 1.0 mg phenanthrene/ml per day to 0.3 mg fluoranthene/ml per da ...19901366395
phosphonate utilization by bacteria in the presence of alternative phosphorus sources.batch and continuous culture experiments were carried out to investigate the effect of orthophosphate and p-nitrophenylphosphate on the utilization of various phosphonates as a p source by bacteria. detailed tests with methylphosphonate as a model phosphonate and the phosphonate-degrading pseudomonas paucimobilis strain mmm101a revealed that, in contrast with the majority of literature data, the phosphates did not suppress phosphonate utilization. under conditions of p stress, strain mmm101a sim ...19901368141
metabolism of naphthalene by the biphenyl-degrading bacterium pseudomonas paucimobilis q1.pseudomonas paucimobilis q1 originally isolated as biphenyl degrading organism (furukawa et al. 1983), was shown to grow with naphthalene. after growth with biphenyl or naphthalene the strain synthesized the same enzyme for the ring cleavage of 2,3-dihydroxybiphenyl or 1,2-dihydroxynaphthalene. the enzyme, although characterized as 2,3-dihydroxybiphenyl dioxygenase (taira et al. 1988), exhibited considerably higher relative activity with 1,2-dihydroxynaphthalene. these results demonstrate that t ...19911368152
cloning of a lignostilbene-alpha,beta-dioxygenase gene from pseudomonas paucimobilis tmy1009. 19911368688
acanthamoeba keratitis: synergy between amebic and bacterial cocontaminants in contact lens care systems as a prelude to infection.we encountered a patient with acanthamoeba keratitis whose contact lens care solution contained numerous trophozoites and cysts admixed with xanthomonas maltophilia organisms, many of which were adherent to the trophozoite surface and internalized within endocytic vacuoles. because of this finding, we investigated the role of bacterial cocontaminants in contact lens care systems as substrates for the growth of acanthamoeba spp. individual cocultivation of acanthamoeba castellanii and a. polyphag ...19921401013
infections with pseudomonas paucimobilis. 19921562669
septic shock caused by pseudomonas paucimobilis. 19921562670
characterization of a beta-lactamase produced by pseudomonas paucimobilis.a novel beta-lactamase enzyme produced by a strain of pseudomonas paucimobilis is described. the enzyme differs from other recorded beta-lactamases from gram-negative aerobic bacteria. it was constitutive, and had the characteristics of a penicillinase. one single band of beta-lactamase activity at pi 4.6 was seen on iso-electric focusing. the enzyme had a molecular mass of 30 kda. the beta-lactamase was strongly inhibited by tazobactam, sulbactam and clavulanic acid but not by the thiol residue ...19911655952
pseudomonas paucimobilis contamination in haemodialysis fluid. 19911672328
molecular cloning of a pseudomonas paucimobilis gene encoding a 17-kilodalton polypeptide that eliminates hcl molecules from gamma-hexachlorocyclohexane.pseudomonas paucimobilis ut26 is capable of growing on gamma-hexachlorocyclohexane (gamma-hch). a genomic library of p. paucimobilis ut26 was constructed in pseudomonas putida by using the broad-host-range cosmid vector pks13. after 2,300 clones were screened by gas chromatography, 3 clones showing gamma-hch degradation were detected. a 5-kb fragment from one of the cosmid clones was subcloned into puc118, and subsequent deletion and gas chromatography-mass spectrometry analyses revealed that a ...19911718942
cloning and sequencing of the gene for a pseudomonas paucimobilis enzyme that cleaves beta-aryl ether.we isolated pseudomonas paucimobilis syk-6, which was able to degrade various dimeric lignin compounds (y. katayama, s. nishikawa, m. nakamura, k. yano, m. yamasaki, n. morohoshi, and t. haraguchi, mokuzai gakkaishi 33:77-79, 1987). this metabolic process is a distinct characteristic of this bacterium, which is equipped with an enzymatic modification system for various dimeric lignin compounds involved in the tricarboxylic acid cycle. cleavage of the beta-aryl ether linkage is essential in this ...19911744051
infections with pseudomonas paucimobilis: report of four cases and review.pseudomonas paucimobilis (formerly cdc group iik, biotype 1) is a strictly aerobic, nonfermenting, oxidase- and catalase-positive, gram-negative bacillus that is widely distributed in water and soil. its name derives from the difficulty encountered in demonstrating its motility, even in liquid media. this microorganism is responsible for two types of infection in humans: sporadic or community-acquired infections, probably of endogenous or environmental origin (bacteremia, meningitis, urinary tra ...19911775840
multiplication of legionella spp. in tap water containing hartmannella vermiformis.a model was developed to study the multiplication of various legionella spp. in tap water containing hartmannella vermiformis. tap water cultures prepared with the following components were suitable for the multiplication studies: legionella spp., 10(3) cfu/ml; h. vermiformis, 10(4.4) cysts per ml; and killed pseudomonas paucimobilis, 10(9) cells per ml. cocultures were incubated at 37 degrees c for at least 1 week. the following legionellae multiplied in tap water cocultures in each replicate e ...19911892386
chemical structure of glycosphingolipids isolated from sphingomonas paucimobilis.two novel glycosphingolipids were isolated from sphingomonas paucimobilis and their structures were completely elucidated. the glycosyl portion of the glycosphingolipid consists of an alpha-d-manp-[1----2)-alpha-d-galp-(1----6)-alpha-d-glcpn-(1 ----4)-alpha-d- glcpa-r tetrasaccharide. the hydrophobic residue r was found to be heterogeneous with respect to the dihydrosphingosine residue. erythro-1,3-dihydroxy-2-amino-octadecane and erythro-1,3-dihydroxy-2-amino-cis-13,14-methyleneoctadecane were ...19911959589
pseudomonas paucimobilis bacteraemia associated with haemodialysis.we report a case of pseudomonas paucimobilis bacteraemia where the source was presumed to be related to haemodialysis. previous reports have identified this organism as a pathogen in a variety of disease states including bacteraemia of unknown origin and in patients undergoing peritoneal dialysis.19901972955
pseudomeningitis caused by pseudomonas paucimobilis. 19912032866
purification and characterization of a 1,2-dihydroxynaphthalene dioxygenase from a bacterium that degrades naphthalenesulfonic acids.1,2-dihydroxynaphthalene dioxygenase was purified to homogeneity from a bacterium that degrades naphthalenesulfonic acids (strain bn6). the enzyme requires fe2+ for maximal activity and consists of eight identical subunits with a molecular weight of about 33,000. analysis of the nh2-terminal amino acid sequence revealed a high degree of homology (22 of 29 amino acids) with the nh2-terminal amino acid sequence of 2,3-dihydroxybiphenyl dioxygenase from strain pseudomonas paucimobilis q1. 1,2-dihyd ...19912050635
contamination during in vitro processing of bone marrow for transplantation: clinical significance.we determined the prevalence and clinical significance of positive microbiologic cultures obtained from 194 consecutive bone marrow harvests intended for infusion in 188 consecutive adult bone marrow transplant patients. microbiologic cultures were obtained at harvest, after manipulation in vitro (for abo imcompatibility or purging procedures), and at the time of thawing and infusion (after earlier cryopreservation). only one of 194 marrow harvests was culture-positive intra-operatively (from an ...19912059759
chemical structure and biological activity of lipooligosaccharide isolated from sphingomonas paucimobilis, a gram-negative bacterium lacking usual lipopolysaccharide. 19902101144
proposals of sphingomonas paucimobilis gen. nov. and comb. nov., sphingomonas parapaucimobilis sp. nov., sphingomonas yanoikuyae sp. nov., sphingomonas adhaesiva sp. nov., sphingomonas capsulata comb. nov., and two genospecies of the genus sphingomonas.based on the partial nucleotide sequence analysis of 16s ribosomal ribonucleic acid (rrna), presence of unique sphingoglycolipids in cellular lipid, and the major type of ubiquinone (q10), we propose sphingomonas gen. nov. with the type species sphingomonas paucimobilis (holmes et al, 1977) comb. nov. from the homology values of deoxyribonucleic acid-deoxyribonucleic acid hybridization and the phenotypic characteristics, three new species, sphingomonas parapaucimobilis, sphingomonas yanoikuyae, ...19902111872
molecular cloning of the protocatechuate 4,5-dioxygenase genes of pseudomonas paucimobilis.we determined the nucleotide sequence of a 1.9-kilobase fragment of pseudomonas paucimobilis syk6 chromosomal dna that included genes encoding protocatechuate 4,5-dioxygenase, the enzyme responsible for the aromatic ring fission of protocatechuate. two open reading frames of 417 and 906 base pairs were found that had no homology with previously reported sequences, including those encoding protocatechuate 3,4-dioxygenase. since both open reading frames were indispensable for the enzyme activity, ...19902185230
pseudomonas paucimobilis peritonitis in a patient on capd successfully treated with ciprofloxacin and netilmicin. 19902209631
isolation and characterization of a fluoranthene-utilizing strain of pseudomonas paucimobilis.a soil bacterium capable of utilizing fluoranthene as the sole source of carbon and energy for growth was purified from a seven-member bacterial community previously isolated from a creosote waste site for its ability to degrade polycyclic aromatic hydrocarbons. by standard bacteriological methods, this bacterium was characterized taxonomically as a strain of pseudomonas paucimobilis and was designated strain epa505. utilization of fluoranthene by strain epa 505 was demonstrated by increase in b ...19902339870
molecular relationship of chromosomal genes encoding biphenyl/polychlorinated biphenyl catabolism: some soil bacteria possess a highly conserved bph operon.all the genes we examined that encoded biphenyl/polychlorinated biphenyl (pcb) degradation were chromosomal, unlike many other degradation-encoding genes, which are plasmid borne. the molecular relationship of genes coding for biphenyl/pcb catabolism in various biphenyl/pcb-degrading pseudomonas, achromobacter, alcaligenes, moraxella, and arthrobacter strains was investigated. among 15 strains tested, 5 pseudomonas strains and one alcaligenes strain possessed the bphabc gene cluster on the xhoi ...19892507526
pseudomonas paucimobilis in purified water for hospital use. 19892575638
detection and localization of a new enzyme catalyzing the beta-aryl ether cleavage in the soil bacterium (pseudomonas paucimobilis syk-6).cleavage of the arylglycerol-beta-aryl ether linkage is the most important process in the biological degradation of lignin. we determined the activity of the enzyme cleaving the beta-aryl ether linkage in membranes of pseudomonas paucimobilis syk-6. this enzyme was tightly associated with the cellular membrane and catalyzed the unique and reductive cleavage of compound ii but not cleavage of compound i. this enzymatic activity was stimulated by addition of nadh. on the basis of this evidence, we ...19892737293
pseudomonas paucimobilis contamination of cool mist tents on a paediatric ward.investigations resulting from the isolation of pseudomonas paucimobilis from the atomizer units of paediatric cool mist tents implicated the hospital water supply as the potential source of contamination. the pathogenic potential of the organism and its role in nosocomial infection is discussed.19862871083
mixed flora brain abscess with pseudomonas paucimobilis after a penetrating lawn dart injury. 19883050862
[aerobic bacterial flora of the nasal cavity of rabbits].on the basis of bacteriological examinations carried out in april 1984 on 60 intranasal swabs, aerobic respiratory microbes were studied in rabbits. differences in flora between animals with and without respiratory diseases were studied. fourteen bacterial species were identified with no difference due to the pathological status. they were: bordetella bronchiseptica, staphylococcus epidermidis, streptococcus faecalis, pasteurella multocida, staphylococcus aureus, bacillus sp., branhamella catarr ...19863096189
cloning and nucleotide sequence of the 2,3-dihydroxybiphenyl dioxygenase gene from the pcb-degrading strain of pseudomonas paucimobilis q1.the bphc gene encoding 2,3-dihydroxybiphenyl dioxygenase was cloned from biphenyl-degrading and chlorinated biphenyl-degrading pseudomonas paucimobilis q1, and its complete nucleotide sequence was determined. the dna-derived protein sequence provides the primary structure of 298 amino acids. polyclonal antibodies raised against this protein from p. paucimobilis q1 failed to cross-react with the previously isolated 2,3-dihydroxybiphenyl dioxygenase from pseudomonas pseudoalcaligenes kf707 [furuka ...19883137968
possible mechanisms responsible for the reduced intestinal flora in hibernating leopard frogs (rana pipiens).mechanisms and factors that normally control the large intestinal flora were investigated to determine whether changes in these parameters could account for the decreased bacterial concentration and facultative nature of the flora found in hibernating frogs. it appeared that low temperatures and limited nutrients were the main factors responsible for the decrease in the bacterial concentration and may also have been responsible for the increase in the proportions of facultative organisms, since ...19883263839
pseudomonas paucimobilis empyema after cardiac transplantation.empyema caused by p paucimobilis and mouth flora occurred in a 56-year-old man two months after orthotopic cardiac transplantation. successful treatment was accomplished with chest tube drainage and four weeks of intravenous cefazolin and clindamycin.19883287645
evaluation of pasco mic-id system for identifying gram-negative bacilli.a production model of the semi-automated pasco mic-id system (pasco laboratories, wheat ridge, colo.) was evaluated with 122 groups or species of gram-negative bacilli, which included typical (499 cultures) and atypical (37 cultures) strains of fermenters and nonfermenters. the pasco system identified 90.9% of 536 cultures accurately; these included 90.8% of 152 nonfermenters, 93.8% of 308 enteric fermenters, and 78.9% of 76 oxidase-positive fermenters. these results were obtained with the aid o ...19873323230
splenic abscess due to clostridium difficile and pseudomonas paucimobilis.extraintestinal infection by clostridium difficile is a rare entity. herein we describe a 62-yr-old man with c. difficile bacteremia complicated by a splenic abscess. of particular interest was the isolation of c. difficile and pseudomonas paucimobilis from the splenic abscess. prompt antibiotic therapy and splenectomy resulted in a favorable outcome. although rare, these organisms should be considered in the differential diagnosis of a splenic abscess.19873565349
[phenotype characteristics of 63 strains of pseudomonas paucimobilis, mostly of hospital origin].sixty-three strains of pseudomonas paucimobilis, a gram-negative, lemon yellow pigmented, glucose nonfermenting bacillus are phenotypically compared to the type strain of this new species (atcc 29837). the morphological, cultural, biochemical (onpg-test, oxidase, aesculin positive) and nutritional (assimilation of many glucides) characteristics and susceptibility or resistance to antimicrobial agents (many antibiotics are active) are reported. the similarities and differences between p. paucimob ...19863706830
community-acquired bloodstream infection caused by pseudomonas paucimobilis: case report and review of the literature.various sources of pseudomonas paucimobilis bacterial infections have been documented. we report the third human case of bloodstream infection due to p. paucimobilis and review the literature in english regarding community-acquired and nosocomial infection due to this bacterium. biochemical and genetic characteristics supporting the pathogenic potential of p. paucimobilis are presented, and the antibiotic susceptibility profile of the organism is summarized.19863771771
an investigation of three commercial methods for rapid identification of non-enteric gram-negative rods. application on pseudomonas paucimobilis and some other pseudomonas species.three commercial systems for the identification of non-enteric gram-negative rods were compared with conventional bacteriological methods as reference. the three systems were the api 20 ne, bio-test id-trident, and rosco diagnostic tablets. the systems were tested on a set of 47 strains from the genus pseudomonas, with the emphasis upon the yellow-pigmented species. the overall identification accuracy was 97% with the api, 19% with the biotest and 68% with the rosco system. the api system was th ...19863788574
peritonitis due to pseudomonas paucimobilis during ambulatory peritoneal dialysis. 19853975764
a numerical taxonomic study of flavobacterium-cytophaga strains from dairy sources.phenotypic data on 203 gram-negative non-fermentative bacteria of the flavobacterium-cytophaga group isolated from milk and butter were analyzed by numerical taxonomic techniques. twenty reference strains including species of flavobacterium, cytophaga and strains of pseudomonas paucimobilis were included in the study. using the matching coefficient of sokal & michener with antibiotic susceptibility data included, 189 isolates were recovered in nine clusters. six of these clusters were linked at ...19854066550
"agrobacterium yellow group" and pseudomonas paucimobilis causing peritonitis in patients receiving continuous ambulatory peritoneal dialysis.five cases of peritonitis occurred during continuous ambulatory peritoneal dialysis in which unusual yellow pigmented gram negative bacteria were isolated in pure culture. four of these isolates were identified as the so called "agrobacterium yellow group." the remaining isolate was identified as pseudomonas paucimobilis, an organism closely related to the agrobacterium yellow group.19854066989
numerical survey of some bacterial taxa.focht, d. d. (iowa state university, ames), and w. r. lockhart. numerical survey of some bacterial taxa. j. bacteriol. 90:1314-1319. 1965.-a numerical analysis was made of 77 properties of each of 43 bacterial strains, representing 25 genera from 8 families in the orders eubacteriales and pseudomonadales. four major groups were found, related to one another at approximately the same level of similarity: (1) a large cluster containing the subgroups (1a) athiorhodaceae-spirillaceae, (1b) xanthomon ...19655848329
differentiation between xanthomonas campestris pv. oryzae, xanthomonas campestris pv. oryzicola and the bacterial 'brown blotch' pathogen on rice by numerical analysis of phenotypic features and protein gel electrophoregrams.thirty-five xanthomonas campestris pv. oryzae, fourteen x. campestris pv. oryzicola strains and six 'brown blotch' pathogens of rice, all of different geographical origin, were studied by numerical analysis of 133 phenotype features and gel electrophoregrams of soluble proteins, %g + c determinations and dna:rrna hybridizations. the following conclusions were drawn. (i) the xanthomonas campestris pathovars oryzae and oryzicola display clearly distinct protein patterns on polyacrylamide gels and ...19846084704
common induction and regulation of biphenyl, xylene/toluene, and salicylate catabolism in pseudomonas paucimobilis.a strain of pseudomonas paucimobilis (strain q1) capable of utilizing biphenyl was isolated from soil. this strain grew not only on substituted biphenyls, but also on salicylate, xylene or toluene or both (xylene/toluene), and substituted benzoates. evidence is presented that the catabolism of biphenyl, xylene/toluene, and salicylate is regulated by a common unit in this strain. the catabolism of biphenyl, xylene/toluene, and salicylate is interrelated, since benzoate and toluate are common meta ...19836343352
lectin-binding activity and antibody response with pseudomonas paucimobilis and flavobacterium multivorum.pseudomonas paucimobilis and flavobacterium multivorum (formerly group iik biotype 1 and biotype 2, respectively) showed lectin-binding activity with helix aspersa and no activity with eight other well-characterized lectins. microagglutination titrations and immunodiffusion precipitation revealed specific antibody activity to immunizing antigens. however, no major antigens were found to be common from crude antigen preparations of p. paucimobilis and f. multivorum when tested against opposing an ...19836411314
pseudomonas paucimobilis peritonitis in patients treated by peritoneal dialysis.pseudomonas paucimobilis has rarely been reported as an opportunistic human pathogen. we report the isolation of this organism in two patients who developed peritonitis during the course of intermittent or continuous ambulatory peritoneal dialysis. the origin of the infection was related to contamination of the dialysate in the first patient but could not be determined in the second case.19846520233
susceptibility of pseudomonas paucimobilis to 24 antimicrobial agents.pseudomonas paucimobilis (group iik, biotype 1) clinical isolates showed in vitro resistance to ampicillin, carbenicillin, cephalothin, cefoxitin, cefamandole, moxalactam, cefotaxime, cefoperazone, mezlocillin, azlocillin, piperacillin, and ticarcillin. those agents to which the microbes were shown to be susceptible were tetracycline, chloramphenicol, gentamicin, tobramycin, kanamycin, amikacin, netilmicin, sisomicin, trimethoprim-sulfamethoxazole, ceftazidime, ceftriaxone, and ceftizoxime.19836600908
immunological nonidentity of pseudomonas paucimobilis with pseudomonas aeruginosa and pseudomonas cepacia.this investigation determined the serum agglutination activity and serum bactericidal response after rabbit immunization with pseudomonas paucimobilis. agglutination activity of antisera showed a twofold increase in titer from before immunization to 4 weeks post-immunization and peaked at 8 weeks post-immunization with a titer of 1:512. 2-mercaptoethanol reduction of immunoglobulin m decreased agglutination titers. no major antigens were found to be common from crude antigen preparations of p. p ...19806783679
sinus contamination with pseudomonas paucimobilis: a pseudoepidemic due to contaminated irrigation fluid.during a five-week period, pseudomonas paucimobilis was isolated from maxillary sinus irrigation washes of four patients who attended an ear, nose and throat clinic. the saline irrigation solution was found to be contaminated with the same organism. further investigation demonstrated the organism in a majority of hospital sinks surveyed. although p. paucimobilis was isolated from clinical specimens, the presence of the organism did not appear to have clinical significance.19816912213
isolation of an unusual 'lipid a' type glycolipid from pseudomonas paucimobilis.a new glycolipid was isolated from defatted cells of pseudomonas paucimobilis iam 12576, and called 'bound lipid'. the 'bound lipid' could not be extracted by hot phenol extraction, but could be extracted with hot chloroform/methanol after hydrolysis with 5% trichloroacetic acid. the 'bound lipid' was purified by thin-layer chromatography on silica gel plates using the solvent mixture chcl3/ch3oh/ch3coooh/ h2o (25:15:4:2, v/v). it consisted of glucosamine, 2-hydroxy myristic acid, galactose, man ...19827126625
enzymatic profile of pseudomonas paucimobilis.pseudomonas paucimobilis, previously known as group iik biotype 1, was found to have a significantly different enzyme profile from that of flavobacterium multivorum, formerly group iik biotype 2. p. paucimobilis produces several esterases and phosphatases which may contribute to its virulence.19827130368
endotoxin-like activity in pseudomonas paucimobilis (group iik biotype 1) and flavobacterium multivorum (group iik biotype 2). 19827151972
[a case of bacteriemia by pseudomonas paucimobilis]. 19827161905
the similarities between pseudomonas paucimobilis and allied bacteria derived from analysis of deoxyribonucleic acids and electrophoretic protein patterns.the chromosomal dna was isolated and purified from 17 strains of pseudomonas paucimobilis, and from the type or reference strains of flavobacterium capsulatum, f. devorans, f. multivorum, 'chromobacterium lividum', xanthomonas campestris and seven species of pseudomonas. the dna base compositions (mol% g + c) of p. paucimobilis strains were between 62.2 and 68.6%, and typical strains had a mean value of 65.3 +/- 0.4 mol%, determined from thermal denaturation temperature. dna-dna molecular hybrid ...19827183747
pseudomonas paucimobilis bacteremia.pseudomonas paucimobilis was isolated from blood of a man after surgery for occlusive vascular disease of his lower extremities. circumstances suggest that the infection was hospital associated and was possibly caused by an organism present in the surroundings of this particularly susceptible host. an environmental source was found. the isolate was susceptible in vitro to carbenicillin, chloramphenicol, tetracycline, and trimethoprim-sulfamethoxazole and was moderately susceptible to amikacin an ...19817251829
outbreak of pseudomonas paucimobilis in an intensive care facility.during a two-month period, six patients in an intensive care unit (icu) were colonized or infected with pseudomonas paucimobilis, a newly described bacterial species. to our knowledge, the epidemic organism has not been found previously in these units. the bacterium was usually recovered from the sputum of persons receiving ventilatory assistance and represented transient colonization. one patient had a symptomatic urinary tract infection. the outbreak was confined to two of the icus. cultures o ...19817253184
[growth inhibition by phenylalanine and tyrosine--metabolism of phenylalanine in flavobacterium devorans (author's transl)].growth of a chloramphenicol-resistant mutant of flavobacterium devorans (f. devorans r) was inhibited by l-phenylalanine and l-tyrosine and furthermore by l-tryptophan, 4-amino-benzoate, anthranilate and homogentisate. the inhibitionwas reversed partially by simultaneous addition of aromatic amino acids and 4-amino-benzoate. after cultivating f. devorans r in a glucose-minimal medium containing l-phenylalanine, the metabolites l-tyrosine, 4-hydroxyphenylpyruvate, 4-hydroxyphenylacetate, 4-hydrox ...19807399402
[a prephenate dehydratase from flavobacterium devorans stimulated by aromatic amino acids (author's transl)].isolation and properties of prephenate dehydratase from flavobacterium devorans r are described. the enzyme was enriched 43-fold by ammonium sulfate precipitation, by gel chromatography on ultrogel aca-34 and by hydrophobic chromatography on decylagarose. the molecular weight was estimated to be 1.35 x 10(5). the enzyme was activated in 1mm solutions of l-tyrosine 4,4-fold, of l-phenylalanine 3.9fold and by l-tryptophan 1.9-fold in 0.02m tris/hcl buffer at ph 7.7. the km values were, when adding ...19807399403
cloning and sequencing of a 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase gene involved in the degradation of gamma-hexachlorocyclohexane in pseudomonas paucimobilis.in pseudomonas paucimobilis ut26, gamma-hexachlorocyclohexane (gamma-hch) is converted to 2,5-dichloro-2,5-cyclohexadiene-1,4-diol (2,5-ddol), which is then metabolized to 2,5-dichlorohydroquinone. here, we isolated from the genomic library of ut26 two genes which expressed 2,5-ddol dehydrogenase activity when they were transformed into p. putida and escherichia coli. both gene products had an apparent molecular size of 28 kda by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the fir ...19947515041
glycosphingolipids from sphingomonas paucimobilis induce monokine production in human mononuclear cells.glycosphingolipids (gsl) isolated from the gram-negative lipopolysaccharide (lps)-free bacterium sphingomonas paucimobilis have remarkable structural similarities with lps and its hydrophobic part, termed lipid a. like lps, but in contrast to the structurally related ceramides and cerebrosides, gsl contain an alpha-linked, negatively charged pyranosidic glycosyl component adjacent to the lipid portion and are capable of forming membranes. because of these similarities, it was of interest to inve ...19957542635
aromatic-degrading sphingomonas isolates from the deep subsurface.an obligately aerobic chemoheterotrophic bacterium (strain f199) previously isolated from southeast coastal plain subsurface sediments and shown to degrade toluene, naphthalene, and other aromatic compounds (j. k. fredrickson, f. j. brockman, d. j. workman, s. w. li, and t. o. stevens, appl. environ. microbiol. 57:796-803, 1991) was characterized by analysis of its 16s rrna nucleotide base sequence and cellular lipid composition. strain f199 contained 2-oh14:0 and 18:1 omega 7c as the predominan ...19957544095
self-induced bacteremia. case report.we describe a patient with perforated appendicitis who postoperatively suffered repeated episodes of shaking chills and temperature spikes. initial blood cultures yielded growth of flavobacterium meningosepticum, pseudomonas putida and pseudomonas paucimobilis, and succeeding blood cultures growth of pseudomonas acidoverans. these bacteria in combination led to a suspicion of self-inoculation of contaminated water through an intravenous catheter. antibiotic treatment had no effect on the symptom ...19957546651
catheter-related bacteremia due to pseudomonas paucimobilis in neutropenic cancer patients: report of two cases. 19957548522
pyrimidine nucleoside catabolism in sphingomonas paucimobilis: role of cytidine deaminase and uridine phosphorylase.pyrimidine nucleoside catabolism in the human pathogen sphingomonas paucimobilis was studied. it was observed that s. paucimobilis was only capable of utilizing cytidine or deoxycytidine as a sole nitrogen source when glucose served as the carbon source. thinlayer chromatographic analyses of cytidine and uridine catabolic products revealed that the enzymes cytidine deaminase and uridine phosphorylase were active in the extracts prepared from s. paucimobilis cells. the levels of cytidine deaminas ...19957600008
microbiological degradation of the herbicide dicamba.pseudomonas paucimobilis was isolated from a consortium which was capable of degrading dicamba (3,6-dichloro-2-methoxybenzoic acid) as the sole source of carbon. the degradation of dicamba by p. paucimobilis and the consortium was examined over a range of substrate concentration, temperature, and ph. in the concentration range of 100-2000 mg dicamba l-1 (0.5-9.0 mm), the degradation was accompanied by a stoichiometric release of 2 mol of cl- per mol of dicamba degraded. the cultures had an optim ...19957612214
screening and characterization of microorganisms with glutaryl-7adca acylase activity.a screening of microorganisms producing glutaryl-7adca acylase, an enzyme able to hydrolyse glutaric acid selectively from glutaryl-3-deacetoxy-7-aminocephalosporanic acid (glutaryl-7adca), has been carried out in soil samples. five microorganisms expressing acylase activity were isolated and classified as bacillus cereus, achromobacter xylosooxidans, bacillus sp., pseudomonas sp. and pseudomonas paucimobilis. the screening was carried out by preparing enrichment cultures containing glutaryl-7-a ...19957632401
bacterial contamination of cellular blood components. a retrospective review at a large cancer center.concern over increased bacterial contamination prompted us to conduct a retrospective review of all bacterial cultures performed on cellular blood components at our institution between december 1989 and june 1993. sterility checks were accomplished by using the bactec blood culture system. the breakdown of the units cultured versus units produced was as follows: packed red blood cells (prbcs): 626 (0.6 percent)/102,593; random donor platelets (rdps): 523 (0.6 percent)/95,005; and single donor pl ...19957668812
isolation and characterization of tn5-induced mutants of pseudomonas paucimobilis ut26 defective in gamma-hexachlorocyclohexane dehydrochlorinase (lina).pseudomonas paucimobilis ut26 grows on gamma-hexachlorocyclohexane (gamma-hch) as a sole source of carbon and energy. tn5 mutation was introduced into ut26, and two kinds of mutants defective in gamma-hch degradation were phenotypically isolated; one (ut64) completely lacked the activity to degrade gamma-hch, while the other (ut61) retained a very low level of activity. tagging and sequencing analysis showed that both mutants had a tn5 insertion at the same site of the lina (gamma-hch dehydrochl ...19937686793
cloning and sequencing of a dehalogenase gene encoding an enzyme with hydrolase activity involved in the degradation of gamma-hexachlorocyclohexane in pseudomonas paucimobilis.in pseudomonas paucimobilis ut26, gamma-hexachlorocyclohexane (gamma-hch) is converted by two steps of dehydrochlorination to a chemically unstable intermediate, 1,3,4,6-tetrachloro-1,4-cyclohexadiene (1,4-tcdn), which is then metabolized to 2,5-dichloro-2,5-cyclohexadiene-1,4-diol (2,5-ddol) by two steps of hydrolytic dehalogenation via the chemically unstable intermediate 2,4,5-trichloro-2,5-cyclohexadiene-1-ol (2,4,5-dnol). to clone a gene encoding the enzyme responsible for the conversion of ...19937691794
cloning, expression, and sequence analysis of a lignostilbene-alpha,beta-dioxygenase gene from pseudomonas paucimobilis tmy1009.from the genomic library of pseudomonas paucimobilis tmy1009 constructed with the cosmid phc79, an 8-kb bamhi-kpni fragment encoding lignostilbene-alpha,beta-dioxygenase (lsd) was cloned into puc19 (designated pksn2510). e. coli jm109 having pksn2510 produced a small amount of lsd only when the lac promoter was induced by isopropyl-beta-d-thio-galactopyranoside (iptg). the 1.9-kb sali fragment containing the lsd gene on pksn2510 was subcloned into puc119 in opposite directions (designated pkhn25 ...19937763879
structural and enzymatical comparison of lignostilbene-alpha,beta-dioxygenase isozymes, i, ii, and iii, from pseudomonas paucimobilis tmy1009.three isozymes of lignostilbene-alpha,beta-dioxygenase (lsd) from pseudomonas paucimobilis tmy1009 were separated on qae-toyopearl chromatography. all active fractions were further chromatographed on deae-toyopearl, butyl-toyopearl, and sephacryl s-300 columns. then the isozymes i, ii, and iii were purified homogeneously. all three isozymes consisted of two subunits with the same mol. mass. according to the n-terminal amino acid sequences up to 25 residues of these three isozymes and the reverse ...19937763880
characterization of the c alpha-dehydrogenase gene involved in the cleavage of beta-aryl ether by pseudomonas paucimobilis.c alpha-dehydrogenase catalyzes the oxidation of arylglycerol-beta-aryl ether at the c alpha-position, and therefore this process produces the specific substrate for beta-etherase, which cleaves beta-ary ethers (c alpha carbonyl type). here we isolated the c alpha-dehydrogenase gene (lig d) and sequenced its nucleotides. this gene contains an open reading frame of 915 bp and the deduced amino acid sequence had a homology with the ribitol dehydrogenase family. lig d is about 1 kbp upstream of the ...19937764263
gellan lyases--novel polysaccharide lyases.a number of bacterial strains capable of degrading the bacterial exopolysaccharide gellan have been isolated by standard enrichment procedures. they include several pink-pigmented gram-negative rod-shaped bacteria. a red-pigmented gram-positive bacillus earlier found to degrade the exopolysaccharide xanthan from xanthomonas campestris also showed slight gellanase activity. all the gram-negative bacteria are non-fermentative, motile and amylase-producing. the gellan degradation in each case is du ...19947812440
bacteriophages from bombyx mori.preparations of silkworm larvae contained two large phages with contractile tails (myoviridae). one phage was active on pseudomonas paucimobilis. the other, not cultivated, was one of the largest viruses known.19947979992
genetic and phenotypic diversity of 2,4-dichlorophenoxyacetic acid (2,4-d)-degrading bacteria isolated from 2,4-d-treated field soils.forty-seven numerically dominant 2,4-dichlorophenoxyacetic acid (2,4-d)-degrading bacteria were isolated at different times from 1989 through 1992 from eight agricultural plots (3.6 by 9.1 m) which were either not treated with 2,4-d or treated with 2,4-d at three different concentrations. isolates were obtained from the most dilute positive most-probable-number tubes inoculated with soil samples from the different plots on seven sampling dates over the 3-year period. the isolates were compared b ...19948017907
use of gene probes to aid in recovery and identification of functionally dominant 2,4-dichlorophenoxyacetic acid-degrading populations in soil.the herbicide 2,4-dichlorophenoxyacetic acid (2,4-d) was applied to soils in microcosms, and degradation was monitored after each of five repeated additions. total dnas were isolated from soil bacterial communities after each 2,4-d treatment. the dna samples were analyzed on slot blots and southern blots by using a tfda gene probe subcloned from plasmid pjp4 and a spa probe derived from a different 2,4-d-degrading isolate, a sphingomonas paucimobilis strain. 2,4-d applied to soil was quickly deg ...19948017908
analysis of competition in soil among 2,4-dichlorophenoxyacetic acid-degrading bacteria.competition among indigenous and inoculated 2,4-dichlorophenoxyacetic acid (2,4-d)-degrading bacteria was studied in a native kansas prairie soil following 2,4-d additions. the soil was inoculated with four different 2,4-d-degrading strains at densities of 10(3) cells per g of soil; the organisms used were pseudomonas cepacia dbo1(pjp4) and three michigan soil isolates, strain 745, sphingomonas paucimobilis 1443, and pseudomonas pickettii 712. following 2,4-d additions, total soil dna was extrac ...19948017909
successful treatment of pseudomonas paucimobilis haemodialysis catheter-related sepsis without catheter removal.infection is the most common complication of long-term central venous access lines. we report a case of central venous haemodialysis catheter infection with pseudomonas paucimobilis, successfully treated by prolonged antibiotic administration without catheter removal. the implications are discussed.19948140021
structure and physical properties of the extracellular polysaccharide ps-p4 produced by sphingomonas paucimobilis p4 (dsm 6418).a new strain, sphingomonas paucimobilis p4 (dsm 6418), was found during a screening programme for exopolysaccharide-producing bacteria. the highly viscous fermentation broth yields a polysaccharide (up to 10 kg/m3), named ps-p4, and shows thixotropic flow behaviour. in the presence of phosphate ions, ps-p4 forms aqueous gels after heating and cooling at alkaline ph. after isolation and purification of the exopolysaccharide, structural analysis by 1d and 2d 1h nmr spectroscopy and mass spectromet ...19948149377
detection of polychlorinated biphenyl degradation genes in polluted sediments by direct dna extraction and polymerase chain reaction.it was the aim of this study to specifically detect the dna sequences for the bphc gene, the meta-cleavage enzyme of the aerobic catabolic pathway for biphenyl and polychlorinated biphenyl degradation, in aquatic sediments without prior cultivation of microorganisms by using extraction of total dna, pcr amplification of bphc sequences, and detection with specific gene probes. the direct dna extraction protocol used was modified to enhance lysis efficiency. crude extracts of dna were further puri ...19938285706
the cell envelope structure of the lipopolysaccharide-lacking gram-negative bacterium sphingomonas paucimobilis.from the cell envelope preparation of sphingomonas paucimobilis two membrane fractions with different densities were separated by sucrose density gradient ultracentrifugation. the high-density fraction contained several major proteins, phospholipids, and glycosphingolipids, which are the only glycolipids of this lipopolysaccharide-lacking gram-negative bacterium. the low-density fraction showed many minor bands of proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and nadh ox ...19948288520
development of field application vectors for bioremediation of soils contaminated with polychlorinated biphenyls.field application vectors (favs), which are a combination of a selective substrate, a host, and a cloning vector, have been developed for the purpose of expressing foreign genes in nonsterile, competitive environments in which the gene products provide no advantage to the host. such gene products are exemplified by the enzymes for the cometabolism of polychlorinated biphenyls (pcbs) through the biphenyl degradation pathway. attempts to use highly competent pcb-cometabolizing strains in the envir ...19938328798
cloning and sequence analysis of a hemolysin-encoding gene from pseudomonas paucimobilis.we report the cloning, expression and nucleotide (nt) sequence of a beta-hemolysin-encoding gene, termed hlya, from pseudomonas paucimobilis. a genomic dna library of the pseudomonad was constructed in escherichia coli using the plasmid vector, puc19. the hlya gene was cloned by screening for a beta-hemolytic phenotype in e. coli transformants and was mapped to a 1100-bp psti-smai fragment. the nt sequence analysis of the 1100-bp insert revealed a 789-bp open reading frame which is preceded by a ...19938344528
classification of rhizomonas suberifaciens, an unnamed rhizomonas species, and sphingomonas spp. in rrna superfamily iv.thermal melting profiles of hybrids between 3h-labeled rrna of rhizomonas suberifaciens, the causal agent of corky root of lettuce, and chromosomal dnas from 27 species of gram-negative bacteria indicated that the genus rhizomonas belongs to superfamily iv of de ley. on the basis of the melting temperatures of dna hybrids with rrnas from the type strains of r. suberifaciens, sphingomonas paucimobilis, and sphingomonas capsulata, rhizomonas strains constitute a separate branch in superfamily iv, ...19938427800
[pseudomonas paucimobilis infections. an analysis of 2 cases]. 19938497747
cloning of a lignostilbene-alpha,beta-dioxygenase isozyme gene from pseudomonas paucimobilis tmy1009.a genomic dna library of pseudomonas paucimobilis tmy1009 was constructed using a cosmid vector pwe15. screening of the library for lignostilbene-alpha,beta-dioxygenase (lsd) isozyme genes was done with a common probe for the alpha, beta, and gamma subunits, which composed lsd isozymes. the positive clones obtained by colony hybridization were further confirmed by southern hybridization. a 4.2-kb bamhi-hindiii fragment hybridized with the probe was subcloned into puc118 to yield the plasmid pkhe ...19958534977
stimulation of phagocytosis and phagosome-lysosome fusion by glycosphingolipids from sphingomonas paucimobilis.sphingomonas paucimobilis, a gram-negative opportunistic pathogen, is actively phagocytosed by human peripheral polymorphonuclear leukocytes (pmn) in vitro. however, when live or killed cells were delipidated, the phagocytic rate was clearly decreased. therefore, we have investigated the physiological role of membrane lipids in phagocytic processes. s. paucimobilis type strain 2395 produces four classes of acidic glycosphingolipids (gl-1, gl-2, gl-3, and gl-4) with the common components of glucu ...19958543558
characterization of pseudomonas paucimobilis fp2001 which forms flagella depending upon the presence of rhamnose in liquid medium.a bacterial strain fp2001 isolated from the exudate of land reclaimed for municipal waste was identified as pseudomonas paucimobilis. cells of strain fp2001 were mobile by means of polar monotrichous flagellum, only when rhamnose was added as a carbon source in the liquid medium. the replacement of rhamnose by arabinose, galactose, glucose or xylose did not lead to the formation of flagella.19958569527
halopicolinic acids, novel products arising through the degradation of chloro- and bromo-biphenyl by sphingomonas paucimobilis bpsi-3.sphingomonas paucimobilis bpsi-3 was previously isolated from a mixed microbial consortium growing on biphenyl as the sole source of carbon and energy. transformation of 4-chlorobiphenyl (4cbp) was demonstrated by this strain, although little or no growth was observed. in minimal salts medium supplemented with 4cbp or bromobiphenyl and dextrose, yellow coloured product(s) were rapidly formed. gas chromatography-mass spectrometry (gc-ms) revealed single-ring n-heterocyclic compounds that were ide ...19968595598
novel polypeptides induced by the insecticide lindane (gamma-hexachlorocyclohexane) are required for its biodegradation by a sphingomonas paucimobilis strain.when exposed to the potent insecticide gamma-hexachlorocyclohexane or lindane, a sphingomonas paucimobilis strain rapidly synthesized 7 novel polypeptides and concomitantly gained the ability to degrade lindane. synthesis of these proteins was switched-off subsequent to the disappearance of lindane from the medium. treatments which induced the synthesis of identical proteins also conferred on cells the ability to degrade lindane. in contrast, cells blocked in protein synthesis could not be induc ...19968630034
direct involvement of hydrogen peroxide in bacterial alpha-hydroxylation of fatty acid.we have reported that fatty-acid alpha-hydroxylase partially purified from sphingomonas paucimobilis required nadh and molecular oxygen. in this study, we found that the reaction was greatly inhibited by catalase. glutathione and glutathione peroxidase also inhibited alpha-hydroxylation, but superoxide dismutase and mannitol did not. replacement of nadh and molecular oxygen by hydrogen peroxide increased the alpha-hydroxylation activity. in the presence of hydrogen peroxide, molecular oxygen was ...19968647293
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